Vanderbilt University School of Medicine

Zaika, Alexander , Ph.D.
Associate Professor of Surgery
Assistant Professor of Cancer Biology

Lab Url: http://Department of Surgery

Phone Number: (615)322-7209

Email Address:alex.zaika@vanderbilt.edu

Zaika, Alexander's picture

Office Address   Mailing Address

1255 Light Hall

Vanderbilt University Medical Center, 1255 Light Hall 37232


Research Keywords
Cancer Research,Tumor Suppressors,Oncogenes,p53 protein family,Apoptosis,Cancer,Cell cycle,Gene regulation,Malignancy,Signal transduction,Transcription,Transcription factor

Research Specialty
Molecular Mechanisms of Tumorigenesis, p53 protein family

Research Description
p53 protein family, which consists of p53, p63 and p73, plays a pivotal role in regulation of multiple cellular mechanisms including apoptosis, cell cycle control and DNA damage repair. We are interested in understanding the molecular and cellular machinery that underlie the functions of these proteins. Although the role of p53 in human studies is relatively well studied, less is known about p73 and p63. Our analyses demonstrated that p73 and p63 family interact with each-other and these interactions play a critical role in human tumorigenesis. Our data also suggest that these proteins are strongly involved in a response to anticancer curative treatment. We are using a number of different in vitro and in vivo techniques to examine the role of the p53 protein family in cancer development and progression.



Publications
Vilgelm, A, Wei, JX, Piazuelo, MB, Washington, MK, Prassolov, V, El-Rifai, W, Zaika, A. DeltaNp73alpha regulates MDR1 expression by inhibiting p53 function. Oncogene, 27(15), 2170-6, 2008.

Wei, J, O''Brien, D, Vilgelm, A, Piazuelo, MB, Correa, P, Washington, MK, El-Rifai, W, Peek, RM, Zaika, A. Interaction of Helicobacter pylori with gastric epithelial cells is mediated by the p53 protein family. Gastroenterology, 134(5), 1412-23, 2008. PMCID:2430883

Tomkova, K, El-Rifai, W, Vilgelm, A, Kelly, MC, Wang, TC, Zaika, AI. The gastrin gene promoter is regulated by p73 isoforms in tumor cells. Oncogene, , , 2006.

Zaika, AI, El-Rifai, W. The role of p53 protein family in gastrointestinal malignancies. Cell Death Differ, 13(6), 935-40, 2006.

Belkhiri, A, Zaika, A, Pidkovka, N, Knuutila, S, Moskaluk, C, El-Rifai, W. Darpp-32: a novel antiapoptotic gene in upper gastrointestinal carcinomas. Cancer Res, 65(15), 6583-92, 2005.

Koon, N, Zaika, A, Moskaluk, CA, Frierson, HF, Knuutila, S, Powell, SM, El-Rifai, W. Clustering of molecular alterations in gastroesophageal carcinomas. Neoplasia, 6(2), 143-9, 2004. PMCID:1502088

Slade, N, Zaika, AI, Erster, S, Moll, UM. DeltaNp73 stabilises TAp73 proteins but compromises their function due to inhibitory hetero-oligomer formation. Cell Death Differ, 11(3), 357-60, 2004.

Tomkova, K, Belkhiri, A, El-Rifai, W, Zaika, AI. p73 isoforms can induce T-cell factor-dependent transcription in gastrointestinal cells. Cancer Res, 64(18), 6390-3, 2004.

Varis, A, Zaika, A, Puolakkainen, P, Nagy, B, Madrigal, I, Kokkola, A, V??yrynen, A, K??rkk??inen, P, Moskaluk, C, El-Rifai, W, Knuutila, S. Coamplified and overexpressed genes at ERBB2 locus in gastric cancer. Int J Cancer, 109(4), 548-53, 2004.

Joseph, TW, Zaika, A, Moll, UM. Nuclear and cytoplasmic degradation of endogenous p53 and HDM2 occurs during down-regulation of the p53 response after multiple types of DNA damage. FASEB J, 17(12), 1622-30, 2003.

Mihara, M, Erster, S, Zaika, A, Petrenko, O, Chittenden, T, Pancoska, P, Moll, UM. p53 has a direct apoptogenic role at the mitochondria. Mol Cell, 11(3), 577-90, 2003.

Petrenko, O, Zaika, A, Moll, UM. deltaNp73 facilitates cell immortalization and cooperates with oncogenic Ras in cellular transformation in vivo. Mol Cell Biol, 23(16), 5540-55, 2003. PMCID:166317

Shirangi, TR, Zaika, A, Moll, UM. Nuclear degradation of p53 occurs during down-regulation of the p53 response after DNA damage. FASEB J, 16(3), 420-2, 2002.

Zaika, AI, Slade, N, Erster, SH, Sansome, C, Joseph, TW, Pearl, M, Chalas, E, Moll, UM. DeltaNp73, a dominant-negative inhibitor of wild-type p53 and TAp73, is up-regulated in human tumors. J Exp Med, 196(6), 765-80, 2002. PMCID:2194062

Moll, UM, Erster, S, Zaika, A. p53, p63 and p73--solos, alliances and feuds among family members. Biochim Biophys Acta, 1552(2), 47-59, 2001.

Moll, UM, Zaika, A. Nuclear and mitochondrial apoptotic pathways of p53. FEBS Lett, 493(2-3), 65-9, 2001.

Sansome, C, Zaika, A, Marchenko, ND, Moll, UM. Hypoxia death stimulus induces translocation of p53 protein to mitochondria. Detection by immunofluorescence on whole cells. FEBS Lett, 488(3), 110-5, 2001.

Zaika, A, Irwin, M, Sansome, C, Moll, UM. Oncogenes induce and activate endogenous p73 protein. J Biol Chem, 276(14), 11310-6, 2001.

Kovalev, S, Mateen, A, Zaika, AI, O''Hea, BJ, Moll, UM. Lack of defective expression of the ATM gene in sporadic breast cancer tissues and cell lines. Int J Oncol, 16(4), 825-31, 2000.

Marchenko, ND, Zaika, A, Moll, UM. Death signal-induced localization of p53 protein to mitochondria. A potential role in apoptotic signaling. J Biol Chem, 275(21), 16202-12, 2000.

Moll, UM, Zaika, A. Disrupting the p53-mdm2 interaction as a potential therapeutic modality. Drug Resist Updat, 3(4), 217-221, 2000.

Zaika, A, Marchenko, N, Moll, UM. Cytoplasmically "sequestered" wild type p53 protein is resistant to Mdm2-mediated degradation. J Biol Chem, 274(39), 27474-80, 1999.

Zaika, A, Mozzherin, DJ, Tan, CK, Downey, KM, Fisher, PA. A two-dimensional support for selective binding of polyhistidine-tagged proteins: identification of a proliferating cell nuclear antigen point mutant with altered function in vitro. Anal Biochem, 268(2), 193-200, 1999.

Zaika, AI, Kovalev, S, Marchenko, ND, Moll, UM. Overexpression of the wild type p73 gene in breast cancer tissues and cell lines. Cancer Res, 59(13), 3257-63, 1999.


Postdoctoral Position Available
Yes

Postdoctoral Position Details
Postdoctoral and Research Assistant positions are available. Individuals who have strong experience in cellular and molecular biology techniques and interest in cancer science are encouraged to apply.

Updated Date
12/11/2012