Vanderbilt University School of Medicine

Zaika, Alexander , Ph.D.
Associate Professor of Surgery

Lab Url: http://Department of Surgery

Phone Number: (615)322-7209

Email Address:alex.zaika@vanderbilt.edu

Zaika, Alexander's picture

Office Address   Mailing Address

1255 Light Hall

Vanderbilt University Medical Center, 1255 Light Hall 37232


Research Keywords
Cancer Research,Tumor Suppressors,Oncogenes,p53 protein family,Apoptosis,Cancer,Cell cycle,Gene regulation,Malignancy,Signal transduction,Transcription,Transcription factor

Research Specialty
Molecular Mechanisms of Tumorigenesis, p53 protein family

Research Description
p53 protein family, which consists of p53, p63 and p73, plays a pivotal role in regulation of multiple cellular mechanisms including apoptosis, cell cycle control and DNA damage repair. We are interested in understanding the molecular and cellular machinery that underlie the functions of these proteins. Although the role of p53 in human studies is relatively well studied, less is known about p73 and p63. Our analyses demonstrated that p73 and p63 family interact with each-other and these interactions play a critical role in human tumorigenesis. Our data also suggest that these proteins are strongly involved in a response to anticancer curative treatment. We are using a number of different in vitro and in vivo techniques to examine the role of the p53 protein family in cancer development and progression.



Publications
Bhardwaj, V, Noto, JM, Wei, J, Andl, C, El-Rifai, W, Peek, RM, Zaika, AI. Helicobacter pylori bacteria alter the p53 stress response via ERK-HDM2 pathway. Oncotarget, 6(3), 1531-43, 2015.

Wei, J, Noto, JM, Zaika, E, Romero-Gallo, J, Piazuelo, MB, Schneider, B, El-Rifai, W, Correa, P, Peek, RM, Zaika, AI. Bacterial CagA protein induces degradation of p53 protein in a p14ARF-dependent manner. Gut, 64(7), 1040-8, 2015.

Zaika, AI. Bacterial Pathogen Helicobacter pylori: A Bad AKTor Inhibits p53 Protein Activity [corrected]. Dig Dis Sci, 60(4), 822-3, 2015.

Zaika, E, Bhardwaj, V, Wei, J, Washington, MK, Souza, R, El-Rifai, W, Zaika, A. Proinflammatory cytokines and bile acids upregulate DeltaNp73 protein, an inhibitor of p53 and p73 tumor suppressors. PLoS One, 8(5), e64306, 2013.

Wei, J, Noto, J, Zaika, E, Romero-Gallo, J, Correa, P, El-Rifai, W, Peek, RM, Zaika, A. Pathogenic bacterium Helicobacter pylori alters the expression profile of p53 protein isoforms and p53 response to cellular stresses. Proc Natl Acad Sci U S A, 109(38), E2543-50, 2012.

Wei, J, Zaika, E, Zaika, A. p53 Family: Role of Protein Isoforms in Human Cancer. J Nucleic Acids, 2012, 687359, 2012.

Zaika, A, Wei, J, Noto, J, Peek, R. Regulation of the p53 tumor suppressor by gastric pathogen Helicobacter pylori pathogen. Oncotarget, , , 2012.

Zaika, E, Wei, J, Yin, D, Andl, C, Moll, U, El-Rifai, W, Zaika, AI. p73 protein regulates DNA damage repair. FASEB J, 25(12), 4406-14, 2011.

Vilgelm, AE, Hong, SM, Washington, MK, Wei, J, Chen, H, El-Rifai, W, Zaika, A. Characterization of DeltaNp73 expression and regulation in gastric and esophageal tumors. Oncogene, 29(43), 5861-8, 2010.

Vilgelm, AE, Washington, MK, Wei, J, Chen, H, Prassolov, VS, Zaika, AI. Interactions of the p53 protein family in cellular stress response in gastrointestinal tumors. Mol Cancer Ther, 9(3), 693-705, 2010.

Wei, J, Nagy, TA, Vilgelm, A, Zaika, E, Ogden, SR, Romero-Gallo, J, Piazuelo, MB, Correa, P, Washington, MK, El-Rifai, W, Peek, RM, Zaika, A. Regulation of p53 tumor suppressor by Helicobacter pylori in gastric epithelial cells. Gastroenterology, 139(4), 1333-43, 2010.

Vilgelm, A, El-Rifai, W, Zaika, A. Therapeutic prospects for p73 and p63: rising from the shadow of p53. Drug Resist Updat, 11(4-5), 152-63, 2008.

Vilgelm, A, Wei, JX, Piazuelo, MB, Washington, MK, Prassolov, V, El-Rifai, W, Zaika, A. DeltaNp73alpha regulates MDR1 expression by inhibiting p53 function. Oncogene, 27(15), 2170-6, 2008.

Wei, J, O''Brien, D, Vilgelm, A, Piazuelo, MB, Correa, P, Washington, MK, El-Rifai, W, Peek, RM, Zaika, A. Interaction of Helicobacter pylori with gastric epithelial cells is mediated by the p53 protein family. Gastroenterology, 134(5), 1412-23, 2008. PMCID:2430883

Zaika, AI, El-Rifai, W. The role of p53 protein family in gastrointestinal malignancies. Cell Death Differ, 13(6), 935-40, 2006.

Tomkova, K, Belkhiri, A, El-Rifai, W, Zaika, AI. p73 isoforms can induce T-cell factor-dependent transcription in gastrointestinal cells. Cancer Res, 64(18), 6390-3, 2004.

Joseph, TW, Zaika, A, Moll, UM. Nuclear and cytoplasmic degradation of endogenous p53 and HDM2 occurs during down-regulation of the p53 response after multiple types of DNA damage. FASEB J, 17(12), 1622-30, 2003.

Mihara, M, Erster, S, Zaika, A, Petrenko, O, Chittenden, T, Pancoska, P, Moll, UM. p53 has a direct apoptogenic role at the mitochondria. Mol Cell, 11(3), 577-90, 2003.

Petrenko, O, Zaika, A, Moll, UM. deltaNp73 facilitates cell immortalization and cooperates with oncogenic Ras in cellular transformation in vivo. Mol Cell Biol, 23(16), 5540-55, 2003. PMCID:166317

Shirangi, TR, Zaika, A, Moll, UM. Nuclear degradation of p53 occurs during down-regulation of the p53 response after DNA damage. FASEB J, 16(3), 420-2, 2002.

Zaika, AI, Slade, N, Erster, SH, Sansome, C, Joseph, TW, Pearl, M, Chalas, E, Moll, UM. DeltaNp73, a dominant-negative inhibitor of wild-type p53 and TAp73, is up-regulated in human tumors. J Exp Med, 196(6), 765-80, 2002. PMCID:2194062

Moll, UM, Erster, S, Zaika, A. p53, p63 and p73--solos, alliances and feuds among family members. Biochim Biophys Acta, 1552(2), 47-59, 2001.

Moll, UM, Zaika, A. Nuclear and mitochondrial apoptotic pathways of p53. FEBS Lett, 493(2-3), 65-9, 2001.

Sansome, C, Zaika, A, Marchenko, ND, Moll, UM. Hypoxia death stimulus induces translocation of p53 protein to mitochondria. Detection by immunofluorescence on whole cells. FEBS Lett, 488(3), 110-5, 2001.

Zaika, A, Irwin, M, Sansome, C, Moll, UM. Oncogenes induce and activate endogenous p73 protein. J Biol Chem, 276(14), 11310-6, 2001.

Kovalev, S, Mateen, A, Zaika, AI, O''Hea, BJ, Moll, UM. Lack of defective expression of the ATM gene in sporadic breast cancer tissues and cell lines. Int J Oncol, 16(4), 825-31, 2000.

Marchenko, ND, Zaika, A, Moll, UM. Death signal-induced localization of p53 protein to mitochondria. A potential role in apoptotic signaling. J Biol Chem, 275(21), 16202-12, 2000.

Moll, UM, Zaika, A. Disrupting the p53-mdm2 interaction as a potential therapeutic modality. Drug Resist Updat, 3(4), 217-221, 2000.

Zaika, A, Mozzherin, DJ, Tan, CK, Downey, KM, Fisher, PA. A two-dimensional support for selective binding of polyhistidine-tagged proteins: identification of a proliferating cell nuclear antigen point mutant with altered function in vitro. Anal Biochem, 268(2), 193-200, 1999.

Zaika, AI, Kovalev, S, Marchenko, ND, Moll, UM. Overexpression of the wild type p73 gene in breast cancer tissues and cell lines. Cancer Res, 59(13), 3257-63, 1999.


Postdoctoral Position Available
Yes

Postdoctoral Position Details
Postdoctoral and Research Assistant positions are available.

Individuals who have strong experience in cellular and molecular biology techniques and interest in cancer science are encouraged to apply.

Updated Date
07/24/2015