Biomedical Research Education & Training
Faculty Member

Chung, Chang Y., Ph.D.
Associate Professor of Medicine

Lab Url: N/A

Phone Number: 615-322-4956

Email Address: chang.chung@vanderbilt.edu

Chung, Chang's picture
Academic history
B.S., Seoul National Univ
M.S., Seoul National Univ
Ph.D., Duke Univ
Postdoc, Univ. of California, San Diego

Office Address   Mailing Address

468 RRB (MRB I)
Vanderbilt Univeristy Medical Center

468 RRB 6600


Research Keywords
Molecular Genetics |Signal Transduction| Signaling Pathways | Cytoskeleton | Cell Motility |Chemotaxis |GPCR | Gene Knockout | Cell Polarity | Cell Migration | Fluorescence Imaging | Cell Biology | Development Dictyostelium ,Biochemistry,Developmental biology,Genetics,Genome,Genomics,Kinase,Knockout,Mutation,Pharmacology,Phosphorylation,Proteomics,Receptor,Signal transduction,Spectroscopy,Tyrosine phosphorylation

Research Specialty
Regulation of cellular polarity and motility during chemotaxis

Research Description

The goal of our laboratory is to understand signaling pathways regulating cell polarity and motility during chemotaxis. Chemotaxis, directed movement towards a chemoattractant agent, is essential for many biological processes such as immune responses, wound healing, axon guidance, and the development of Dictyostelium. Inflammation is a key component of pathophysiology of both acute injuries and chronic neurological diseases including Parkinson???s and Alzheimer???s. Inflammation in neuronal injuries and neurodegeneration involves important roles for resident inflammatory cells known as microglia. Microglia chemotaxis is prerequisite for microglia function whether it is neuroprotective or inflammatory. Therefore, understanding microglial chemotaxis is essential for designing rational approaches for therapeutic modulation of this response, and better understanding of the regulation of microglia proliferation and chemotaxis has important therapeutic implications. However, it is not fully understood how the activation and chemotaxis of resident microglia in the brain is controlled at early stage of neuroinflammation. Chemoattractant (extracelluar ATP or ADP) released from damaged neurons and astrocytes induces microglia activation and chemotaxis through multiple receptors including G??i/o-coupled P2Y12 receptor (P2Y12R). The intracellular signaling pathway downstream of P2Y12R during microglia chemotaxis is poorly understood. Recently, we have undertaken the responsibility of unraveling complex networks of signaling pathways downstream of P2Y12R during microglia chemotaxis.


Publications
Chung, CY, Feoktistov, A, Hollingsworth, RJ, Rivero, F, Mandel, NS. An attenuating role of a WASP-related protein, WASP-B, in the regulation of F-actin polymerization and pseudopod formation via the regulation of RacC during Dictyostelium chemotaxis. Biochem Biophys Res Commun, 436(4), 719-24, 2013

Wang, D, Dasari, S, Chambers, MC, Holman, JD, Chen, K, Liebler, DC, Orton, DJ, Purvine, SO, Monroe, ME, Chung, CY, Rose, KL, Tabb, DL. Basophile: Accurate Fragment Charge State Prediction Improves Peptide Identification Rates. Genomics Proteomics Bioinformatics, 2013

Lee, SH, Hollingsworth, R, Kwon, HY, Lee, N, Chung, CY. I?-arrestin 2-dependent activation of ERK1/2 is required for ADP-induced paxillin phosphorylation at Ser(83) and microglia chemotaxis. Glia, 60(9), 1366-77, 2012

Lee, SH, Schneider, C, Higdon, AN, Darley-Usmar, VM, Chung, CY. Role of iPLA(2) in the Regulation of Src Trafficking and Microglia Chemotaxis. Traffic, 12(7), 878-89, 2011

Gruver, JS, Potdar, AA, Jeon, J, Sai, J, Anderson, B, Webb, D, Richmond, A, Quaranta, V, Cummings, PT, Chung, CY. Bimodal analysis reveals a general scaling law governing nondirected and chemotactic cell motility. Biophys J, 99(2), 367-76, 2010

Lin, WH, Nelson, SE, Hollingsworth, RJ, Chung, CY. Functional roles of VASP phosphorylation in the regulation of chemotaxis and osmotic stress response. Cytoskeleton (Hoboken), 67(4), 259-71, 2010

Lee, S, Chung, CY. Role of VASP phosphorylation for the regulation of microglia chemotaxis via the regulation of focal adhesion formation/maturation. Mol Cell Neurosci, 42(4), 382-390, 2009

Lee, S, Han, JW, Leeper, L, Gruver, JS, Chung, CY. Regulation of the formation and trafficking of vesicles from Golgi by PCH family proteins during chemotaxis. Biochim Biophys Acta, 1793, 1199-1209, 2009 PMCID:2703453

Gruver, JS, Wikswo, JP, Chung, CY. 3''-phosphoinositides regulate the coordination of speed and accuracy during chemotaxis. Biophys J, 95(8), 4057-67, 2008 PMCID:2553113

Han, JW, Leeper, L, Rivero, F, Chung, CY. Role of RacC for the regulation of WASP and phosphatidylinositol 3-kinase during chemotaxis of Dictyostelium. J Biol Chem, 281(46), 35224-34, 2006

Myers, SA, Leeper, LR, Chung, CY. WASP-interacting Protein (WIPa) Is Important for Actin Filament Elongation and Prompt Pseudopod Formation in Response to a Dynamic Chemoattractant Gradient. Mol Biol Cell, 17, 4564-4575, 2006 PMCID:1635341

Myers, SA, Han, JW, Lee, Y, Firtel, RA, Chung, CY. A Dictyostelium homologue of WASP is required for polarized F-actin assembly during chemotaxis. Mol Biol Cell, 16(5), 2191-206, 2005 PMCID:1087228

Walker, GM, Sai, J, Richmond, A, Stremler, M, Chung, CY, Wikswo, JP. Effects of flow and diffusion on chemotaxis studies in a microfabricated gradient generator. Lab Chip, 5(6), 611-8, 2005 PMCID:2665276

Chung, CY, Firtel, RA. Signaling pathways at the leading edge of chemotaxing cells. J Muscle Res Cell Motil, 23(7-8), 773-9, 2002

Han, YH, Chung, CY, Wessels, D, Stephens, S, Titus, MA, Soll, DR, Firtel, RA. Requirement of a vasodilator-stimulated phosphoprotein family member for cell adhesion, the formation of filopodia, and chemotaxis in dictyostelium. J Biol Chem, 277(51), 49877-87, 2002

Chung, CY, Funamoto, S, Firtel, RA. Signaling pathways controlling cell polarity and chemotaxis. Trends Biochem Sci, 26(9), 557-66, 2001

Chung, CY, Potikyan, G, Firtel, RA. Control of cell polarity and chemotaxis by Akt/PKB and PI3 kinase through the regulation of PAKa. Mol Cell, 7(5), 937-47, 2001

Chien, S, Chung, CY, Sukumaran, S, Osborne, N, Lee, S, Ellsworth, C, McNally, JG, Firtel, RA. The Dictyostelium LIM domain-containing protein LIM2 is essential for proper chemotaxis and morphogenesis. Mol Biol Cell, 11(4), 1275-91, 2000 PMCID:14846

Chung, C.Y. Firtel, R.A.. Dictyostelium: A model experimental system for elucidating the pathways and mechanisms controlling chemotaxis.. Molecular Regulation, The Humana Press, 99-114, 2000

Chung, CY, Lee, S, Briscoe, C, Ellsworth, C, Firtel, RA. Role of Rac in controlling the actin cytoskeleton and chemotaxis in motile cells. Proc Natl Acad Sci U S A, 97(10), 5225-30, 2000 PMCID:25810

Firtel, RA, Chung, CY. The molecular genetics of chemotaxis: sensing and responding to chemoattractant gradients. Bioessays, 22(7), 603-15, 2000

Chung, CY, Firtel, RA. PAKa, a putative PAK family member, is required for cytokinesis and the regulation of the cytoskeleton in Dictyostelium discoideum cells during chemotaxis. J Cell Biol, 147(3), 559-76, 1999 PMCID:2151188

Chung, CY, Reddy, TB, Zhou, K, Firtel, RA. A novel, putative MEK kinase controls developmental timing and spatial patterning in Dictyostelium and is regulated by ubiquitin-mediated protein degradation. Genes Dev, 12(22), 3564-78, 1998 PMCID:317245


Postdoctoral Position Available
No

Postdoctoral Position Details
N/A

Updated Date
01/06/2014