Biomedical Research Education & Training
Faculty Member

Slebos, Robbert J. C., PhD, MHS
Research Associate Professor of Biochemistry
Research Associate Professor of Cancer Biology

Lab Url: N/A

Phone Number: 615-936-2806

Email Address:

Slebos, Robbert's picture
Academic history
Ph.D., Univ of Amsterdam
B.Sc., Stichting Opleiding Leraren, Utrecht
M.Sc., Univ of Utrecht
M.H.S., Duke University

Office Address   Mailing Address

U1213A, Medical Research Building III

Ayers Institute U1213A, Medical Research Building III 37232

Research Keywords
Translational Research, Proteomics, Colorectal Cancer, Head and Neck Cancer

Research Specialty
Translational research using shotgun proteomics for the detection of biomarkers of early detection, response prediction and prognosis of colorectal cancer and head and neck cancer.

Chung, CH, Aulino, J, Muldowney, NJ, Hatakeyama, H, Baumann, J, Burkey, B, Netterville, J, Sinard, R, Yarbrough, WG, Cmelak, AJ, Slebos, RJ, Shyr, Y, Parker, J, Gilbert, J, Murphy, BA. Nuclear factor-kappa B pathway and response in a phase II trial of bortezomib and docetaxel in patients with recurrent and/or metastatic head and neck squamous cell carcinoma. Ann Oncol, 21(4), 864-70, 2010 PMCID:2920032

Dasari, S, Chambers, MC, Slebos, RJ, Zimmerman, LJ, Ham, AJ, Tabb, DL. TagRecon: high-throughput mutation identification through sequence tagging. J Proteome Res, 9(4), 1716-26, 2010 PMCID:2920032

Hatakeyama, H, Cheng, H, Wirth, P, Counsell, A, Marcrom, SR, Wood, CB, Pohlmann, PR, Gilbert, J, Murphy, B, Yarbrough, WG, Wheeler, DL, Harari, PM, Guo, Y, Shyr, Y, Slebos, RJ, Chung, CH. Regulation of heparin-binding EGF-like growth factor by miR-212 and acquired cetuximab-resistance in head and neck squamous cell carcinoma. PLoS One, 5(9), e12702, 2010 PMCID:2938338

Li, M, Gray, W, Zhang, H, Chung, CH, Billheimer, D, Yarbrough, WG, Liebler, DC, Shyr, Y, Slebos, RJ. Comparative shotgun proteomics using spectral count data and quasi-likelihood modeling. J Proteome Res, 9(8), 4295-305, 2010 PMCID:2920032

Lin, D, Li, J, Slebos, RJ, Liebler, DC. Cysteinyl peptide capture for shotgun proteomics: global assessment of chemoselective fractionation. J Proteome Res, 9(10), 5461-72, 2010 PMCID:2948434

Tu, C, Rudnick, PA, Martinez, MY, Cheek, KL, Stein, SE, Slebos, RJ, Liebler, DC. Depletion of abundant plasma proteins and limitations of plasma proteomics. J Proteome Res, 9(10), 4982-91, 2010 PMCID:2948434

Baumann, JL, Cohen, S, Evjen, AN, Law, JH, Vadivelu, S, Attia, A, Schindler, JS, Chung, CH, Wirth, PS, Meijer, CJ, Snijders, PJ, Yarbrough, WG, Slebos, RJ. Human papillomavirus in early laryngeal carcinoma. Laryngoscope, 119(8), 1531-7, 2009 PMCID:2920032

Fiske, WH, Tanksley, J, Nam, KT, Goldenring, JR, Slebos, RJ, Liebler, DC, Abtahi, AM, La Fleur, B, Ayers, GD, Lind, CD, Washington, MK, Coffey, RJ. Efficacy of cetuximab in the treatment of Menetrier''s disease. Sci Transl Med, 1(8), 8ra18, 2009 PMCID:2920032

Sprung, RW, Brock, JW, Tanksley, JP, Li, M, Washington, MK, Slebos, RJ, Liebler, DC. Equivalence of protein inventories obtained from formalin-fixed paraffin-embedded and frozen tissue in multidimensional liquid chromatography-tandem mass spectrometry shotgun proteomic analysis. Mol Cell Proteomics, 8(8), 1988-98, 2009 PMCID:2722776

Chung, CH, Mirakhur, B, Chan, E, Le, QT, Berlin, J, Morse, M, Murphy, BA, Satinover, SM, Hosen, J, Mauro, D, Slebos, RJ, Zhou, Q, Gold, D, Hatley, T, Hicklin, DJ, Platts-Mills, TA. Cetuximab-induced anaphylaxis and IgE specific for galactose-alpha-1,3-galactose. N Engl J Med, 358(11), 1109-17, 2008 PMCID:2361129

Luo, W, Slebos, RJ, Hill, S, Li, M, Br?!bek, J, Amanchy, R, Chaerkady, R, Pandey, A, Ham, AJ, Hanks, SK. Global impact of oncogenic Src on a phosphotyrosine proteome. J Proteome Res, 7(8), 3447-60, 2008 PMCID:2579752

Palka, KT, Slebos, RJ, Chung, CH. Update on molecular diagnostic tests in head and neck cancer. Semin Oncol, 35(3), 198-210, 2008 PMCID:2490629

Slebos, RJ, Brock, JW, Winters, NF, Stuart, SR, Martinez, MA, Li, M, Chambers, MC, Zimmerman, LJ, Ham, AJ, Tabb, DL, Liebler, DC. Evaluation of strong cation exchange versus isoelectric focusing of peptides for multidimensional liquid chromatography-tandem mass spectrometry. J Proteome Res, 7(12), 5286-94, 2008 PMCID:2669493

Slebos, RJ, Li, M, Vadivelu, S, Burkey, BB, Netterville, JL, Sinard, R, Gilbert, J, Murphy, B, Chung, CH, Shyr, Y, Yarbrough, WG. Microsatellite mutations in buccal cells are associated with aging and head and neck carcinoma. Br J Cancer, 98(3), 619-26, 2008 PMCID:2243146

Flake, GP, Rivera, MP, Funkhouser, WK, Maygarden, SJ, Meadows, KL, Long, EH, Stockton, PS, Jones, TC, Yim, HW, Slebos, RJ, Taylor, JA. Detection of Pre-Invasive Lung Cancer: Technical Aspects of the LIFE Project. Toxicol Pathol, 35(1), 65-74, 2007

Yarbrough, WG, Whigham, A, Brown, B, Roach, M, Slebos, R. Phosphoinositide kinase-3 status associated with presence or absence of human papillomavirus in head and neck squamous cell carcinomas. Int J Radiat Oncol Biol Phys, 69(2 Suppl), S98-101, 2007

Yim, HW, Slebos, RJ, Randell, SH, Umbach, DM, Parsons, AM, Rivera, MP, Detterbeck, FC, Taylor, JA. Smoking is associated with increased telomerase activity in short-term cultures of human bronchial epithelial cells. Cancer Lett, 246(1-2), 24-33, 2007

Chung, CH, Ely, K, McGavran, L, Varella-Garcia, M, Parker, J, Parker, N, Jarrett, C, Carter, J, Murphy, BA, Netterville, J, Burkey, BB, Sinard, R, Cmelak, A, Levy, S, Yarbrough, WG, Slebos, RJ, Hirsch, FR. Increased epidermal growth factor receptor gene copy number is associated with poor prognosis in head and neck squamous cell carcinomas. J Clin Oncol, 24(25), 4170-6, 2006

Chung, CH, Parker, JS, Ely, K, Carter, J, Yi, Y, Murphy, BA, Ang, KK, El-Naggar, AK, Zanation, AM, Cmelak, AJ, Levy, S, Slebos, RJ, Yarbrough, WG. Gene Expression Profiles Identify Epithelial-to-Mesenchymal Transition and Activation of Nuclear Factor-{kappa}B Signaling as Characteristics of a High-risk Head and Neck Squamous Cell Carcinoma. Cancer Res, 66(16), 8210-8218, 2006

Slebos, RJ, Li, M, Evjen, AN, Coffa, J, Shyr, Y, Yarbrough, WG. Mutagenic effect of cadmium on tetranucleotide repeats in human cells. Mutat Res, 602(1-2), 92-9, 2006

Slebos, RJ, Yi, Y, Ely, K, Carter, J, Evjen, A, Zhang, X, Shyr, Y, Murphy, BM, Cmelak, AJ, Burkey, BB, Netterville, JL, Levy, S, Yarbrough, WG, Chung, CH. Gene expression differences associated with human papillomavirus status in head and neck squamous cell carcinoma. Clin Cancer Res, 12(3 Pt 1), 701-9, 2006

Yarbrough, WG, Slebos, RJ, Liebler, D. Proteomics: clinical applications for head and neck squamous cell carcinoma. Head Neck, 28(6), 549-58, 2006

Slebos, RJ, Livanos, E, Yim, HW, Randell, SH, Parsons, AM, Detterbeck, FC, Rivera, MP, Taylor, JA. Chromosomal abnormalities in bronchial epithelium from smokers, nonsmokers, and lung cancer patients. Cancer Genet Cytogenet, 159(2), 137-42, 2005

Slebos, RJ, Little, RE, Umbach, DM, Antipkin, Y, Zadaorozhnaja, TD, Mendel, NA, Sommer, CA, Conway, K, Parrish, E, Gulino, S, Taylor, JA. Mini-and microsatellite mutations in children from Chernobyl accident cleanup workers. Mutat Res, 559(1-2), 143-51, 2004

Slebos, Robbert J C, Umbach, David M, Sommer, Courtney A, Horner, Geoffrey A, Choi, Jane Y, Taylor, Jack A. Analytical and statistical methods to evaluate microsatellite allelic imbalance in small amounts of DNA. Lab Invest, 2004

Jin, Yong Hwan, Clark, Alan B, Slebos, Robbert J C, Al-Refai, Hanan, Taylor, Jack A, Kunkel, Thomas A, Resnick, Michael A, Gordenin, Dmitry A, . Cadmium is a mutagen that acts by inhibiting mismatch repair. Nat Genet, 34, 326-9, 2003 PMCID:2662193

Slebos, Robbert J C, Oh, Daniel S, Umbach, David M, Taylor, Jack A, . Mutations in tetranucleotide repeats following DNA damage depend on repeat sequence and carcinogenic agent. Cancer Res, 62, 6052-60, 2002

Slebos, R J, Taylor, J A, . A novel host cell reactivation assay to assess homologous recombination capacity in human cancer cell lines. Biochem Biophys Res Commun, 281, 212-9, 2001

Lax, S F, Kendall, B, Tashiro, H, Slebos, R J, Hedrick, L. The frequency of p53, K-ras mutations, and microsatellite instability differs in uterine endometrioid and serous carcinoma: evidence of distinct molecular genetic pathways.. Cancer, 88(4), 814-24, 2000

Slebos, R J, Hoppin, J A, Tolbert, P E, Holly, E A, Brock, J W, Zhang, R H, Bracci, P M, Foley, J, Stockton, P, McGregor, L M, Flake, G P, Taylor, J A, . K-ras and p53 in pancreatic cancer: association with medical history, histopathology, and environmental exposures in a population-based study. Cancer Epidemiol Biomarkers Prev, 9, 1223-32, 2000

Ceha, H M, Clement, M J, Polak, M M, Offerhaus, G J, Slebos, R J. Mutational analysis of the P16-binding domain of cyclin-dependent kinase 4 in tumors in the head region of the pancreas.. Pancreas, 17(1), 85-8, 1998

Ceha, H M, Nasser, I, Medema, R H, Slebos, R J. Several noncontiguous domains of CDK4 are involved in binding to the P16 tumor suppressor protein.. Biochem Biophys Res Commun, 249(2), 550-5, 1998

Slebos, R J, Baas, I O, Clement, M J, Offerhaus, G J, Askin, F B, Hruban, R H, Westra, W H. p53 alterations in atypical alveolar hyperplasia of the human lung.. Hum Pathol, 29(8), 801-8, 1998

Slebos, R J, Resnick, M A, Taylor, J A. Inactivation of the p53 tumor suppressor gene via a novel Alu rearrangement.. Cancer Res, 58(23), 5333-6, 1998

Wilentz, R E, Chung, C H, Sturm, P D, Musler, A, Sohn, T A, Offerhaus, G J, Yeo, C J, Hruban, R H, Slebos, R J. K-ras mutations in the duodenal fluid of patients with pancreatic carcinoma.. Cancer, 82(1), 96-103, 1998

Dijkhuizen, S M, Entius, M M, Clement, M J, Polak, M M, Van den Berg, F M, Craanen, M E, Slebos, R J, Offerhaus, G J. Multiple hyperplastic polyps in the stomach: evidence for clonality and neoplastic potential.. Gastroenterology, 112(2), 561-6, 1997

Entius, M M, Westerman, A M, Giardiello, F M, van Velthuysen, M L, Polak, M M, Slebos, R J, Wilson, J H, Hamilton, S R, Offerhaus, G J. Peutz-Jeghers polyps, dysplasia, and K-ras codon 12 mutations.. Gut, 41(3), 320-2, 1997 PMCID:1891488

Hruban, R H, Sturm, P D, Slebos, R J, Wilentz, R E, Musler, A R, Yeo, C J, Sohn, T A, van Velthuysen, M L, Offerhaus, G J. Can K-ras codon 12 mutations be used to distinguish benign bile duct proliferations from metastases in the liver? A molecular analysis of 101 liver lesions from 93 patients.. Am J Pathol, 151(4), 943-9, 1997 PMCID:1858056

Rodenhuis, S, Boerrigter, L, Top, B, Slebos, R J, Mooi, W J, van't Veer, L, van Zandwijk, N. Mutational activation of the K-ras oncogene and the effect of chemotherapy in advanced adenocarcinoma of the lung: a prospective study.. J Clin Oncol, 15(1), 285-91, 1997

Baas, I O, van den Berg, F M, Mulder, J W, Clement, M J, Slebos, R J, Hamilton, S R, Offerhaus, G J. Potential false-positive results with antigen enhancement for immunohistochemistry of the p53 gene product in colorectal neoplasms.. J Pathol, 178(3), 264-7, 1996

Chung, C H, Wilentz, R E, Polak, M M, Ramsoekh, T B, Noorduyn, L A, Gouma, D J, Huibregtse, K, Offerhaus, G J, Slebos, R J. Clinical significance of K-ras oncogene activation in ampullary neoplasms.. J Clin Pathol, 49(6), 460-4, 1996 PMCID:500534

Slebos, R J, Baas, I O, Clement, M, Polak, M, Mulder, J W, van den Berg, F M, Hamilton, S R, Offerhaus, G J. Clinical and pathological associations with p53 tumour-suppressor gene mutations and expression of p21WAF1/Cip1 in colorectal carcinoma.. Br J Cancer, 74(2), 165-71, 1996 PMCID:2074586

Westra, W H, Baas, I O, Hruban, R H, Askin, F B, Wilson, K, Offerhaus, G J, Slebos, R J. K-ras oncogene activation in atypical alveolar hyperplasias of the human lung.. Cancer Res, 56(9), 2224-8, 1996

Offerhaus, G J, Slebos, R J. [Molecular-genetic detection of colorectal carcinoma in the feces]. Ned Tijdschr Geneeskd, 139(52), 2714-5, 1995

Slebos, R J, Kessis, T D, Chen, A W, Han, S M, Hedrick, L, Cho, K R, . Functional consequences of directed mutations in human papillomavirus E6 proteins: abrogation of p53-mediated cell cycle arrest correlates with p53 binding and degradation in vitro. Virology, 208, 111-20, 1995

Kern, J A, Slebos, R J, Top, B, Rodenhuis, S, Lager, D, Robinson, R A, Weiner, D, Schwartz, D A. C-erbB-2 expression and codon 12 K-ras mutations both predict shortened survival for patients with pulmonary adenocarcinomas.. J Clin Invest, 93(2), 516-20, 1994 PMCID:293872

Slebos, R J, Lee, M H, Plunkett, B S, Kessis, T D, Williams, B O, Jacks, T, Hedrick, L, Kastan, M B, Cho, K R. p53-dependent G1 arrest involves pRB-related proteins and is disrupted by the human papillomavirus 16 E7 oncoprotein.. Proc Natl Acad Sci U S A, 91(12), 5320-4, 1994 PMCID:43986

Kessis, T D, Slebos, R J, Han, S M, Shah, K, Bosch, X F, Mu??oz, N, Hedrick, L, Cho, K R. p53 gene mutations and MDM2 amplification are uncommon in primary carcinomas of the uterine cervix.. Am J Pathol, 143(5), 1398-405, 1993 PMCID:1887168

Kessis, T D, Slebos, R J, Nelson, W G, Kastan, M B, Plunkett, B S, Han, S M, Lorincz, A T, Hedrick, L, Cho, K R. Human papillomavirus 16 E6 expression disrupts the p53-mediated cellular response to DNA damage.. Proc Natl Acad Sci U S A, 90(9), 3988-92, 1993 PMCID:46431

Slichenmyer, W J, Nelson, W G, Slebos, R J, Kastan, M B. Loss of a p53-associated G1 checkpoint does not decrease cell survival following DNA damage.. Cancer Res, 53(18), 4164-8, 1993

Westra, W H, Offerhaus, G J, Goodman, S N, Slebos, R J, Polak, M, Baas, I O, Rodenhuis, S, Hruban, R H. Overexpression of the p53 tumor suppressor gene product in primary lung adenocarcinomas is associated with cigarette smoking.. Am J Surg Pathol, 17(3), 213-20, 1993

Westra, W H, Slebos, R J, Offerhaus, G J, Goodman, S N, Evers, S G, Kensler, T W, Askin, F B, Rodenhuis, S, Hruban, R H. K-ras oncogene activation in lung adenocarcinomas from former smokers. Evidence that K-ras mutations are an early and irreversible event in the development of adenocarcinoma of the lung.. Cancer, 72(2), 432-8, 1993

Rodenhuis, S, Slebos, R J. Clinical significance of ras oncogene activation in human lung cancer.. Cancer Res, 52(9 Suppl), 2665s-2669s, 1992

Slebos, R J, Boerrigter, L, Evers, S G, Wisman, P, Mooi, W J, Rodenhuis, S. A rapid and simple procedure for the routine detection of ras point mutations in formalin-fixed, paraffin-embedded tissues.. Diagn Mol Pathol, 1(2), 136-41, 1992

Slebos, R J, Rodenhuis, S. The ras gene family in human non-small-cell lung cancer.. J Natl Cancer Inst Monogr(13), 23-9, 1992

Breuer, M, Wientjens, E, Verbeek, S, Slebos, R, Berns, A. Carcinogen-induced lymphomagenesis in pim-1 transgenic mice: dose dependence and involvement of myc and ras. Cancer Res, 51(3), 958-63, 1991

Oudejans, J J, Slebos, R J, Zoetmulder, F A, Mooi, W J, Rodenhuis, S. Differential activation of ras genes by point mutation in human colon cancer with metastases to either lung or liver.. Int J Cancer, 49(6), 875-9, 1991

Rygaard, K, Slebos, R J, Spang-Thomsen, M. Radiosensitivity of small-cell lung cancer xenografts compared with activity of c-myc, N-myc, L-myc, c-raf-1 and K-ras proto-oncogenes.. Int J Cancer, 49(2), 279-84, 1991

Slebos, R J, Habets, G G, Evers, S G, Mooi, W J, Rodenhuis, S. Allele-specific detection of K-ras oncogene expression in human non-small-cell lung carcinomas.. Int J Cancer, 48(1), 51-6, 1991

Slebos, R J, Hruban, R H, Dalesio, O, Mooi, W J, Offerhaus, G J, Rodenhuis, S. Relationship between K-ras oncogene activation and smoking in adenocarcinoma of the human lung.. J Natl Cancer Inst, 83(14), 1024-7, 1991

Rodenhuis, S, Slebos, R J. The ras oncogenes in human lung cancer.. Am Rev Respir Dis, 142(6 Pt 2), S27-30, 1990

Slebos, R J, Kibbelaar, R E, Dalesio, O, Kooistra, A, Stam, J, Meijer, C J, Wagenaar, S S, Vanderschueren, R G, van Zandwijk, N, Mooi, W J. K-ras oncogene activation as a prognostic marker in adenocarcinoma of the lung.. N Engl J Med, 323(9), 561-5, 1990

Breuer, M, Slebos, R, Verbeek, S, van Lohuizen, M, Wientjens, E, Berns, A. Very high frequency of lymphoma induction by a chemical carcinogen in pim-1 transgenic mice. Nature, 340(6228), 61-3, 1989

Slebos, R J, Evers, S G, Wagenaar, S S, Rodenhuis, S. Cellular protoonocogenes are infrequently amplified in untreated non-small cell lung cancer.. Br J Cancer, 59(1), 76-80, 1989 PMCID:2246978

Slebos, R J, Rodenhuis, S. The molecular genetics of human lung cancer.. Eur Respir J, 2(5), 461-9, 1989

H??ppener, J W, Mosselman, S, Roholl, P J, Lambrechts, C, Slebos, R J, de Pagter-Holthuizen, P, Lips, C J, Jansz, H S, Sussenbach, J S. Expression of insulin-like growth factor-I and -II genes in human smooth muscle tumours.. EMBO J, 7(5), 1379-85, 1988 PMCID:458387

Rodenhuis, S, Slebos, R J, Boot, A J, Evers, S G, Mooi, W J, Wagenaar, S S, van Bodegom, P C, Bos, J L. Incidence and possible clinical significance of K-ras oncogene activation in adenocarcinoma of the human lung.. Cancer Res, 48(20), 5738-41, 1988

Slebos, R J, de Graeff, A, Van Zandwijk, N, Mooi, W J, Bos, J L, Rodenhuis, S. Recurrent breast cancer and an adenocarcinoma of the lung occurring in one patient: c-myc oncogene amplification and K-ras codon 12 point mutation as tumour markers.. Eur J Cancer Clin Oncol, 24(9), 1529-30, 1988

de Graeff, A, Slebos, R J, Rodenhuis, S. Resistance to cisplatin and analogues: mechanisms and potential clinical implications.. Cancer Chemother Pharmacol, 22(4), 325-32, 1988

H??ppener, J W, Steenbergh, P H, Slebos, R J, Visser, A, Lips, C J, Jansz, H S, Bechet, J M, Lenoir, G M, Born, W, Haller-Brem, S. Expression of the second calcitonin/calcitonin gene-related peptide gene in Ewing sarcoma cell lines.. J Clin Endocrinol Metab, 64(4), 809-17, 1987

H??ppener, J W, Steenbergh, P H, Slebos, R J, de Pagter-Holthuizen, P, Roos, B A, Jansen, M, Van den Brande, J L, Sussenbach, J S, Jansz, H S, Lips, C J. Expression of insulin-like growth factor-I and -II genes in rat medullary thyroid carcinoma.. FEBS Lett, 215(1), 122-6, 1987

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