Biomedical Research Education & Training
Faculty Member

Williams, John V., M.D.
Associate Professor of Pediatrics
Associate Professor of Pathology, Microbiology and Immunology

Lab Url: http://pediatrics.mc.vanderbilt.edu/interior.php?mid=5333

Phone Number: 615-936-2186

Email Address: john.williams@vanderbilt.edu

Williams, John's picture
Academic history
B.S., University of Virginia
M.D., Medical College of Virginia
Internship and residency, Children's Hospital of Pittsburgh
Fellowship, Vanderbilt University Medical Center

Office Address   Mailing Address

D-7206 MCN; lab phone 936-1011

D-7235 MCN 37232-2581


Research Keywords
paramyxoviruses, metapneumovirus,Infectious disease,Microbiology,Virus

Research Specialty
The Williams laboratory studies the cell entry, pathogenesis and immunity of human metapneumovirus, a leading cause of respiratory disease worldwide.

Research Description
The Williams laboratory studies the immunology and pathogenesis of human metapneumovirus and other respiratory viruses. Human metapneumovirus (hMPV) is a novel paramyxovirus that was only discovered in 2001. Numerous studies by our group and others have shown that hMPV is a leading cause of acute lower respiratory tract illness in infants and children worldwide. There are no licensed antiviral or vaccines for hMPV and little is known about protective immunity and viral pathogenesis. We have developed animal models for hMPV, purified prototype viral strains, generated recombinant viral proteins and identified a cellular receptor. Our research is in three major areas: a) pathogenesis and immunity in the animal models; b) cell entry of hMPV; c) structure and function of the fusion protein. We seek both to understand the immunobiology of this important human pathogen, and to facilitate the development of preventive and therapeutic strategies.

Publications
Carroll, KN, Gebretsadik, T, Minton, P, Woodward, K, Liu, Z, Miller, EK, Williams, JV, Dupont, WD, Hartert, TV. Influence of maternal asthma on the cause and severity of infant acute respiratory tract infections. J Allergy Clin Immunol, 129(5), 1236-42, 2012 PMCID:3358080

Carroll, KN, Griffin, MR, Edwards, KM, Ali, A, Zhu, Y, Iwane, MK, Szilagyi, PG, Staat, MA, Stevens, TP, Hall, CB, Williams, JV, Hartert, TV. Adherence to Guidelines for Respiratory Syncytial Virus Immunoprophylaxis among Infants with Prematurity or Chronic Lung Disease in Three United States Counties. Pediatr Infect Dis J, 2012 PMCID:3408742

Chien, YW, Vidal, JE, Grijalva, CG, Bozio, C, Edwards, KM, Williams, JV, Griffin, MR, Verastegui, H, Hartinger, SM, Gil, AI, Lanata, CF, Klugman, KP. Density Interactions between Streptococcus pneumoniae, Haemophilus influenzae and Staphylococcus aureus in the Nasopharynx of Young Peruvian Children. Pediatr Infect Dis J, 2012

Cox, RG, Livesay, SB, Johnson, M, Ohi, MD, Williams, JV. The Human Metapneumovirus Fusion Protein Mediates Entry Via an Interaction with RGD-binding Integrins. J Virol, 2012

Erickson, JJ, Gilchuk, P, Hastings, AK, Tollefson, SJ, Johnson, M, Downing, MB, Boyd, KL, Johnson, JE, Kim, AS, Joyce, S, Williams, JV. Viral acute lower respiratory infections impair CD8+ T cells through PD-1. J Clin Invest, 122(8), 2967-82, 2012 PMCID:3408742

Jules, A, Grijalva, CG, Zhu, Y, Talbot, KH, Williams, JV, Dupont, WD, Edwards, KM, Schaffner, W, Shay, DK, Griffin, MR. Estimating age-specific influenza-related hospitalization rates during the pandemic (H1N1) 2009 in Davidson Co, TN. Influenza Other Respi Viruses, 6(3), e63-71, 2012 PMCID:3358080

Klemenc, J, Asad Ali, S, Johnson, M, Tollefson, SJ, Talbot, HK, Hartert, TV, Edwards, KM, Williams, JV. Real-time reverse transcriptase PCR assay for improved detection of human metapneumovirus. J Clin Virol, 54(4), 371-5, 2012 PMCID:3408742

Miller, EK, Hernandez, JZ, Wimmenauer, V, Shepherd, BE, Hijano, D, Libster, R, Serra, ME, Bhat, N, Batalle, JP, Mohamed, Y, Reynaldi, A, Rodriguez, A, Otello, M, Pisapia, N, Bugna, J, Bellabarba, M, Kraft, D, Coviello, S, Ferolla, FM, Chen, A, London, SJ, Siberry, GK, Williams, JV, Polack, FP. A mechanistic role for type III IFN-I>1 in asthma exacerbations mediated by human rhinoviruses. Am J Respir Crit Care Med, 185(5), 508-16, 2012 PMCID:3280462

Self, WH, Grijalva, CG, Zhu, Y, Talbot, HK, Jules, A, Widmer, KE, Edwards, KM, Williams, JV, Shay, DK, Griffin, MR. Emergency department visits for influenza A(H1N1)pdm09, Davidson County, Tennessee, USA. Emerg Infect Dis, 18(5), 863-5, 2012 PMCID:3358080

Self, WH, McNaughton, CD, Grijalva, CG, Zhu, Y, Chappell, JD, Williams, JV, Talbot, HK, Shay, DK, Griffin, MR. Diagnostic performance of the BinaxNow Influenza A&B rapid antigen test in ED patients. Am J Emerg Med, 2012 PMCID:3408742

Shaikh, FY, Cox, RG, Lifland, AW, Hotard, AL, Williams, JV, Moore, ML, Santangelo, PJ, Crowe, JE. A critical phenylalanine residue in the respiratory syncytial virus fusion protein cytoplasmic tail mediates assembly of internal viral proteins into viral filaments and particles. MBio, 3(1), 2012 PMCID:3280462

Thomsen, I, Dulek, DE, Creech, CB, Graham, TB, Williams, JV. Chronic granulomatous disease masquerading as Beh??et disease: a case report and review of the literature. Pediatr Infect Dis J, 31(5), 529-31, 2012 PMCID:3280462

Wen, X, Krause, JC, Leser, GP, Cox, RG, Lamb, RA, Williams, JV, Crowe, JE, Jardetzky, TS. Structure of the human metapneumovirus fusion protein with neutralizing antibody identifies a pneumovirus antigenic site. Nat Struct Mol Biol, 19(4), 461-3, 2012

Ali, SA, Gern, JE, Hartert, TV, Edwards, KM, Griffin, MR, Miller, EK, Gebretsadik, T, Pappas, T, Lee, WM, Williams, JV. Real-world comparison of two molecular methods for detection of respiratory viruses. Virol J, 8, 332, 2011 PMCID:3154182

Bordelon, H, Adams, NM, Klemm, AS, Russ, PK, Williams, JV, Talbot, HK, Wright, DW, Haselton, FR. Development of a low-resource RNA extraction cassette based on surface tension valves. ACS Appl Mater Interfaces, 3(6), 2161-8, 2011 PMCID:3129697

Griffin, MR, Monto, AS, Belongia, EA, Treanor, JJ, Chen, Q, Chen, J, Talbot, HK, Ohmit, SE, Coleman, LA, Lofthus, G, Petrie, JG, Meece, JK, Hall, CB, Williams, JV, Gargiullo, P, Berman, L, Shay, DK, , . Effectiveness of non-adjuvanted pandemic influenza A vaccines for preventing pandemic influenza acute respiratory illness visits in 4 U.S. communities. PLoS One, 6(8), e23085, 2011 PMCID:3155536

McElroy, JA, Monahan, SH, Williams, JV. A female neonate presenting with fever and rash. Clin Pediatr (Phila), 50(8), 779-81, 2011 PMCID:3071231

Miller, EK, Williams, JV, Gebretsadik, T, Carroll, KN, Dupont, WD, Mohamed, YA, Morin, LL, Heil, L, Minton, PA, Woodward, K, Liu, Z, Hartert, TV. Host and viral factors associated with severity of human rhinovirus-associated infant respiratory tract illness. J Allergy Clin Immunol, 127(4), 883-91, 2011

Monsalvo, AC, Batalle, JP, Lopez, MF, Krause, JC, Klemenc, J, Hernandez, JZ, Maskin, B, Bugna, J, Rubinstein, C, Aguilar, L, Dalurzo, L, Libster, R, Savy, V, Baumeister, E, Aguilar, L, Cabral, G, Font, J, Solari, L, Weller, KP, Johnson, J, Echavarria, M, Edwards, KM, Chappell, JD, Crowe, JE, Williams, JV, Melendi, GA, Polack, FP. Severe pandemic 2009 H1N1 influenza disease due to pathogenic immune complexes. Nat Med, 17(2), 195-9, 2011

Piyaratna, R, Tollefson, SJ, Williams, JV. Genomic analysis of four human metapneumovirus prototypes. Virus Res, 160(1-2), 200-5, 2011 PMCID:3347971

Stokes, KL, Chi, MH, Sakamoto, K, Newcomb, DC, Currier, MG, Huckabee, MM, Lee, S, Goleniewska, K, Pretto, C, Williams, JV, Hotard, A, Sherrill, TP, Peebles, RS, Moore, ML. Differential Pathogenesis of Respiratory Syncytial Virus (RSV) Clinical Isolates in BALB/c Mice. J Virol, 2011

Talbot, HK, Griffin, MR, Chen, Q, Zhu, Y, Williams, JV, Edwards, KM. Effectiveness of seasonal vaccine in preventing confirmed influenza-associated hospitalizations in community dwelling older adults. J Infect Dis, 203(4), 500-8, 2011

Ali, SA, Williams, JV, Chen, Q, Faori, S, Shehabi, A, Jundi, EA, Khuri-Bulos, N, Halasa, N. Human metapneumovirus in hospitalized children in Amman, Jordan. J Med Virol, 82(6), 1012-6, 2010

Dulek, DE, Williams, JV, Creech, CB, Schulert, AK, Frangoul, HA, Domm, J, Denison, MR, Chappell, JD. Use of intravenous zanamivir after development of oseltamivir resistance in a critically Ill immunosuppressed child infected with 2009 pandemic influenza A (H1N1) virus. Clin Infect Dis, 50(11), 1493-6, 2010

Hartert, TV, Carroll, K, Gebretsadik, T, Woodward, K, Minton, P, , . The Tennessee Children''s Respiratory Initiative: Objectives, design and recruitment results of a prospective cohort study investigating infant viral respiratory illness and the development of asthma and allergic diseases. Respirology, 15(4), 691-9, 2010

Ryder, AB, Tollefson, SJ, Podsiad, AB, Johnson, JE, Williams, JV. Soluble recombinant human metapneumovirus G protein is immunogenic but not protective. Vaccine, 28(25), 4145-52, 2010

Talbot, HK, Williams, JV, Zhu, Y, Poehling, KA, Griffin, MR, Edwards, KM. Failure of routine diagnostic methods to detect influenza in hospitalized older adults. Infect Control Hosp Epidemiol, 31(7), 683-8, 2010

Tollefson, SJ, Cox, RG, Williams, JV. Studies of culture conditions and environmental stability of human metapneumovirus. Virus Res, 151(1), 54-9, 2010

Williams, JV, Edwards, KM, Weinberg, GA, Griffin, MR, Hall, CB, Zhu, Y, Szilagyi, PG, Wang, CK, Yang, CF, Silva, D, Ye, D, Spaete, RR, Crowe, JE. Population-based incidence of human metapneumovirus infection among hospitalized children. J Infect Dis, 201(12), 1890-8, 2010

Cseke, G, Maginnis, MS, Cox, RG, Tollefson, SJ, Podsiad, AB, Wright, DW, Dermody, TS, Williams, JV. Integrin alphavbeta1 promotes infection by human metapneumovirus. Proc Natl Acad Sci U S A, 106(5), 1566-71, 2009 PMCID:2629439

Miller, EK, Edwards, KM, Weinberg, GA, Iwane, MK, Griffin, MR, Hall, CB, Zhu, Y, Szilagyi, PG, Morin, LL, Heil, LH, Lu, X, Williams, JV, , . A novel group of rhinoviruses is associated with asthma hospitalizations. J Allergy Clin Immunol, 123(1), 98-104.e1, 2009

Miller, EK, Khuri-Bulos, N, Williams, JV, Shehabi, AA, Faouri, S, Al Jundi, I, Chen, Q, Heil, L, Mohamed, Y, Morin, LL, Ali, A, Halasa, NB. Human rhinovirus C associated with wheezing in hospitalised children in the Middle East. J Clin Virol, 46(1), 85-9, 2009

Moore, ML, Chi, MH, Luongo, C, Lukacs, NW, Polosukhin, VV, Huckabee, MM, Newcomb, DC, Buchholz, UJ, Crowe, JE, Goleniewska, K, Williams, JV, Collins, PL, Peebles, RS. A chimeric A2 strain of respiratory syncytial virus (RSV) with the fusion protein of RSV strain line 19 exhibits enhanced viral load, mucus, and airway dysfunction. J Virol, 83(9), 4185-94, 2009 PMCID:2668460

Sato, M, Li, H, Ikizler, MR, Werkhaven, JA, Williams, JV, Chappell, JD, Tang, YW, Wright, PF. Detection of viruses in human adenoid tissues by use of multiplex PCR. J Clin Microbiol, 47(3), 771-3, 2009 PMCID:2650959

Talbot, HK, Crowe, JE, Edwards, KM, Griffin, MR, Zhu, Y, Weinberg, GA, Szilagyi, PG, Hall, CB, Podsiad, AB, Iwane, M, Williams, JV, , . Coronavirus infection and hospitalizations for acute respiratory illness in young children. J Med Virol, 81(5), 853-6, 2009

Talbot, HK, Poehling, KA, Williams, JV, Zhu, Y, Chen, Q, McNabb, P, Edwards, KM, Griffin, MR. Influenza in older adults: impact of vaccination of school children. Vaccine, 27(13), 1923-7, 2009 PMCID:2670238

Talbot, HK, Shepherd, BE, Crowe, JE, Griffin, MR, Edwards, KM, Podsiad, AB, Tollefson, SJ, Wright, PF, Williams, JV. The pediatric burden of human coronaviruses evaluated for twenty years. Pediatr Infect Dis J, 28(8), 682-7, 2009

Williams, JV, Weitkamp, JH, Blum, DL, Lafleur, BJ, Crowe, JE. The human neonatal B cell response to respiratory syncytial virus uses a biased antibody variable gene repertoire that lacks somatic mutations. Mol Immunol, 2009

Yang, CF, Wang, CK, Tollefson, SJ, Piyaratna, R, Lintao, LD, Chu, M, Liem, A, Mark, M, Spaete, RR, Crowe, JE, Williams, JV. Genetic diversity and evolution of human metapneumovirus fusion protein over twenty years. Virol J, 6, 138, 2009 PMCID:2753315

Derdowski, A, Peters, TR, Glover, N, Qian, R, Utley, TJ, Burnett, A, Williams, JV, Spearman, P, Crowe, JE. Human metapneumovirus nucleoprotein and phosphoprotein interact and provide the minimal requirements for inclusion body formation. J Gen Virol, 89(Pt 11), 2698-708, 2008

Mok, H, Tollefson, SJ, Podsiad, AB, Shepherd, BE, Polosukhin, VV, Johnston, RE, Williams, JV, Crowe, JE. An alphavirus replicon-based human metapneumovirus vaccine is immunogenic and protective in mice and cotton rats. J Virol, 82(22), 11410-8, 2008 PMCID:2573258

Mehlmann, M, Bonner, AB, Williams, JV, Dankbar, DM, Moore, CL, Kuchta, RD, Podsiad, AB, Tamerius, JD, Dawson, ED, Rowlen, KL. Comparison of the MChip to viral culture, reverse transcription-PCR, and the QuickVue influenza A+B test for rapid diagnosis of influenza. J Clin Microbiol, 45(4), 1234-7, 2007 PMCID:1865827

Williams, JV, Chen, Z, Cseke, G, Wright, DW, Keefer, CJ, Tollefson, SJ, Hessell, A, Podsiad, A, Shepherd, BE, Sanna, PP, Burton, DR, Crowe, JE, Williamson, RA. A recombinant human monoclonal antibody to human metapneumovirus fusion protein that neutralizes virus in vitro and is effective therapeutically in vivo. J Virol, 81(15), 8315-24, 2007 PMCID:1951312

Cseke, G, Wright, DW, Tollefson, SJ, Johnson, JE, Crowe, JE, Williams, JV. Human Metapneumovirus Fusion Protein Vaccines that are Immunogenic and Protective in Cotton Rats. J Virol, 2006 PMCID:1797435

Miller, SA, Tollefson, S, Crowe, JE, Williams, JV, Wright, DW. Examination of a Fusogenic Hexameric Core from Human Metapneumovirus and Identification of a Potent Synthetic Peptide Inhibitor from the Heptad Repeat 1 Region. J Virol, 2006 PMCID:1797239

Williams, JV, Heymann, PW, Crowe, JE. Reply. J Allergy Clin Immunol, 117(1), 223-4, 2006

Williams, JV, Tollefson, SJ, Nair, S, Chonmaitree, T. Association of human metapneumovirus with acute otitis media. Int J Pediatr Otorhinolaryngol, 2006 PMCID:1476701

Williams, JV, Wang, CK, Yang, CF, Tollefson, SJ, House, FS, Heck, JM, Chu, M, Brown, JB, Lintao, LD, Quinto, JD, Chu, D, Spaete, RR, Edwards, KM, Wright, PF, Crowe, JE. The role of human metapneumovirus in upper respiratory tract infections in children: a 20-year experience. J Infect Dis, 193(3), 387-95, 2006 PMCID:1586246

Halasa, NB, Williams, JV, Wilson, GJ, Walsh, WF, Schaffner, W, Wright, PF. Medical and economic impact of a respiratory syncytial virus outbreak in a neonatal intensive care unit. Pediatr Infect Dis J, 24(12), 1040-4, 2005

Martino, R, Porras, RP, Rabella, N, Williams, JV, R?!mila, E, Margall, N, Labeaga, R, Crowe, JE, Coll, P, Sierra, J. Prospective study of the incidence, clinical features, and outcome of symptomatic upper and lower respiratory tract infections by respiratory viruses in adult recipients of hematopoietic stem cell transplants for hematologic malignancies. Biol Blood Marrow Transplant, 11(10), 781-96, 2005

Shimizu, C, Shike, H, Baker, SC, Garcia, F, van der Hoek, L, Kuijpers, TW, Reed, SL, Rowley, AH, Shulman, ST, Talbot, HK, Williams, JV, Burns, JC. Human coronavirus NL63 is not detected in the respiratory tracts of children with acute Kawasaki disease. J Infect Dis, 192(10), 1767-71, 2005

Tai, JH, Williams, JV, Edwards, KM, Wright, PF, Crowe, JE, Dermody, TS. Prevalence of reovirus-specific antibodies in young children in Nashville, Tennessee. J Infect Dis, 191(8), 1221-4, 2005

Williams, JV. Human Metapneumovirus: An Important Cause of Respiratory Disease in Children and Adults. Curr Infect Dis Rep, 7(3), 204-210, 2005

Williams, JV. The clinical presentation and outcomes of children infected with newly identified respiratory tract viruses. Infect Dis Clin North Am, 19(3), 569-84, 2005

Williams, JV, Crowe, JE, Enriquez, R, Minton, P, Peebles, RS, Hamilton, RG, Higgins, S, Griffin, M, Hartert, TV. Human metapneumovirus infection plays an etiologic role in acute asthma exacerbations requiring hospitalization in adults. J Infect Dis, 192(7), 1149-53, 2005 PMCID:1476781

Williams, JV, Martino, R, Rabella, N, Otegui, M, Parody, R, Heck, JM, Crowe, JE. A prospective study comparing human metapneumovirus with other respiratory viruses in adults with hematologic malignancies and respiratory tract infections. J Infect Dis, 192(6), 1061-5, 2005 PMCID:1483060

Williams, JV, Tollefson, SJ, Heymann, PW, Carper, HT, Patrie, J, Crowe, JE. Human metapneumovirus infection in children hospitalized for wheezing. J Allergy Clin Immunol, 115(6), 1311-2, 2005 PMCID:1476700

Williams, JV, Tollefson, SJ, Johnson, JE, Crowe, JE. The cotton rat (Sigmodon hispidus) is a permissive small animal model of human metapneumovirus infection, pathogenesis, and protective immunity. J Virol, 79(17), 10944-51, 2005 PMCID:1193579

Williams, John V, Harris, Paul A, Tollefson, Sharon J, Halburnt-Rush, Lisa L, Pingsterhaus, Joyce M, Edwards, Kathryn M, Wright, Peter F, Crowe, James E. Human metapneumovirus and lower respiratory tract disease in otherwise healthy infants and children. N Engl J Med, 350(5), 443-50, 2004 PMCID:1831873

Crowe, James E, Williams, John V. Immunology of viral respiratory tract infection in infancy. Paediatr Respir Rev, 4(2), 112-9, 2003

Weitkamp, Jorn-Hendrik, Kallewaard, Nicole, Kusuhara, Koichi, Bures, Elizabeth, Williams, John V, LaFleur, Bonnie, Greenberg, Harry B, Crowe, James E. Infant and adult human B cell responses to rotavirus share common immunodominant variable gene repertoires. J Immunol, 171(9), 4680-8, 2003

Barber, S R, Werdel, J, Symbula, M, Williams, J, Burkett, B A, Taylor, P T, Roche, J K, Crum, C P. Seroreactivity to HPV-16 proteins in women with early cervical neoplasia. Cancer Immunol Immunother, 35(1), 33-8, 1992

Ward, B E, Saleh, A M, Williams, J V, Zitz, J C, Crum, C P. Papillary immature metaplasia of the cervix: a distinct subset of exophytic cervical condyloma associated with HPV-6/11 nucleic acids. Mod Pathol, 5(4), 391-5, 1992

Andersen, W A, Franquemont, D W, Williams, J, Taylor, P T, Crum, C P. Vulvar squamous cell carcinoma and papillomaviruses: two separate entities. Am J Obstet Gynecol, 165(2), 329-35; discussion 335-6, 1991


Postdoctoral Position Available
No

Postdoctoral Position Details
N/A

Updated Date
10/01/2012