Biomedical Research Education & Training
Faculty Member

May, James M., M.D.
Professor of Medicine
Professor of Molecular Physiology and Biophysics

Lab Url: http://www.mc.vanderbilt.edu/root/vumc.php?site=jamesmaylab&doc=30842

Phone Number: 615-936-1665

Email Address: james.may@vanderbilt.edu

May, James's picture
Academic history
B.S., Yale College, New Haven, CT
M.D., VanderbiltVanderbilt Univ. Med. School, Nashville, TN
Internship, Vanderbilt University, Nashville, TN
Residency, Johns Hopkins Hospital, Baltimore, MD
Fellowship, University of Washington, Seattle, WA

Office Address   Mailing Address

7435F MRB IV

7465 MRB IV 2213 Garland Ave 37232-0475


Research Keywords
Antioxidants, Vitamin C, Vitamin E, Selecum, Oxidant Stress,Glucose Trasport, Insulin Action, Hexokinase

Research Description
Dr. May's laboratory is involved in three major areas involving antioxidant vitamins and micronutrients: the function of vitamin C in atherosclerosis; study of the role of vitamin C in protection against the oxidant stress of Alzheimer's disease, and study of the antioxidant interactions of selenium and vitamins C and E.

Dr. May's research interests invove the antioxidant effects of vitamins C and E in a variety of cell and animal models. His laboratory has long been active in the study of how vitamin C is taken up, maintained in a reduced form, and how it protects cells against oxidant stress. In the last several years, the effort has targeted the early stages of atherosclerosis, especially related to endothelial dysfunction and the ability of the vitamin to enhance release and function of nitric oxide. More recently, efforts have turned to testing whether the vitamin also plays a role in inhibiting the atherosclerotic process in other cells, including macrophages and vascular smooth muscle cells. The central hypothesis in this work is that the vitamin C transporter is crucial for maintaining high intracellular concentrations of this vitamin. Other functions of vitamin C besides those related to antioxidant mechanisms are also explored, including its role in cell proliferation, differentiation, and collagen formation.

Alzheimer's disease is associated with oxidant stress, and vitamin C has been implicated in clinical studies in protecting against this stress and in slowing progression of the disease. Studies are underway to assess the role of the vitamin in beta-amyloid secretion and action by cultured neuronal cells, and to asses whether dietary manipulation with vitamin C in normal mice and mice unable to synthesize the vitamin can slow the progression of the behavioral changes seen in animals transgenic for amyloid precursor protein and presenilin 1. Both of these proteins are implicated in many patients with Alzheimer's disease.

Selenium is a co-factor for several enzymes with antioxidant function, including glutathione peroxidase and thioredoxin reductase. Dr. May's laboratory showed that the latter enzyme can reduce the oxidized forms of vitamin C back to the reduced form. The ability of this selenoenzyme to recycle vitamin C may provide a missing link between the known ability of vitamin E or selenium to mitigate a deficiency in the other. In cultured cells and in guinea pigs (which can be made deficient in all three factors), studies are underway of the interactions between these factors with regard to cellular metabolism and lipid peroxidation. The approaches used in these studies include HPLC assays of antioxidants, enzyme assays, and assays of lipid peroxidation.

Publications
Qiu, S, Li, L, Weeber, EJ, May, JM. Ascorbate transport by primary cultured neurons and its role in neuronal function and protection against excitotoxicity. J Neurosci Res, 85(5), 1046-56, 2007

Burk, RF, Christensen, JM, Maguire, MJ, Austin, LM, Whetsell, WO, May, JM, Hill, KE, Ebner, FF. A combined deficiency of vitamins E and C causes severe central nervous system damage in guinea pigs. J Nutr, 136(6), 1576-81, 2006

Huang, J, May, JM. Ascorbic acid protects SH-SY5Y neuroblastoma cells from apoptosis and death induced by beta-amyloid. Brain Res, 1097(1), 52-8, 2006

May, JM, Li, L, Hayslett, K, Qu, ZC. Ascorbate transport and recycling by SH-SY5Y neuroblastoma cells: response to glutamate toxicity. Neurochem Res, 31(6), 785-94, 2006

May, JM, Qu, ZC, Nelson, DJ. Cellular disulfide-reducing capacity: an integrated measure of cell redox capacity. Biochem Biophys Res Commun, 344(4), 1352-9, 2006

May, JM, Huang, J, Qu, ZC. Macrophage uptake and recycling of ascorbic acid: response to activation by lipopolysaccharide. Free Radic Biol Med, 39(11), 1449-59, 2005

May, JM, Li, L, Qu, ZC, Huang, J. Ascorbate uptake and antioxidant function in peritoneal macrophages. Arch Biochem Biophys, 440(2), 165-72, 2005

May, JM, Qu, ZC. Transport and intracellular accumulation of vitamin C in endothelial cells: relevance to collagen synthesis. Arch Biochem Biophys, 434(1), 178-86, 2005

May, JM, Qu, ZC, Juliao, S, Cobb, CE. Ascorbic acid decreases oxidant stress in endothelial cells caused by the nitroxide tempol. Free Radic Res, 39(2), 195-202, 2005

Huang, J, de Paulis, T, May, JM. Antioxidant effects of dihydrocaffeic acid in human EA.hy926 endothelial cells. J Nutr Biochem, 15(12), 722-9, 2004

May, JM, Qu, ZC. Nitric oxide-induced oxidant stress in endothelial cells: amelioration by ascorbic acid. Arch Biochem Biophys, 429(1), 106-13, 2004

May, JM, Qu, ZC, Cobb, CE. Human erythrocyte recycling of ascorbic acid: relative contributions from the ascorbate free radical and dehydroascorbic acid. J Biol Chem, 279(15), 14975-82, 2004

May, JM, Qu, ZC, Cobb, CE. Reduction and uptake of methylene blue by human erythrocytes. Am J Physiol Cell Physiol, 286(6), C1390-8, 2004

May, JM, Qu, ZC, Li, X. Nitrite generates an oxidant stress and increases nitric oxide in EA.hy926 endothelial cells. Free Radic Res, 38(6), 581-9, 2004

Steffner, RJ, Wu, L, Powers, AC, May, JM. Ascorbic acid recycling by cultured beta cells: effects of increased glucose metabolism. Free Radic Biol Med, 37(10), 1612-21, 2004

Wu, L, Nicholson, W, Knobel, SM, Steffner, RJ, May, JM, Piston, DW, Powers, AC. Oxidative stress is a mediator of glucose toxicity in insulin-secreting pancreatic islet cell lines. J Biol Chem, 279(13), 12126-34, 2004

Hardy, T.A. and May, J.M. Coordinate regulation of L-arginine uptake and nitric oxide synthase activity in cultured endothelial cells. Free Radic. Biol. Med., 2002, in press.

Hill, KE, Montine, TJ, Motley, AK, Li, X, May, JM, Burk, RF. Combined deficiency of vitamins E and C causes paralysis and death in guinea pigs. Am J Clin Nutr, 77(6), 1484-8, 2003

Huang, J, May, JM. Ascorbic acid spares alpha-tocopherol and prevents lipid peroxidation in cultured H4IIE liver cells. Mol Cell Biochem, 247(1-2), 171-6, 2003

Li, X, Huang, J, May, JM. Ascorbic acid spares alpha-tocopherol and decreases lipid peroxidation in neuronal cells. Biochem Biophys Res Commun, 305(3), 656-61, 2003

Li, X, May, JM. Location and recycling of mitochondrial alpha-tocopherol. Mitochondrion, 3(1), 29-38, 2003

May, JM, Qu, ZC, Li, X. Ascorbic acid blunts oxidant stress due to menadione in endothelial cells. Arch Biochem Biophys, 411(1), 136-44, 2003

May, JM, Qu, ZC, Neel, DR, Li, X. Recycling of vitamin C from its oxidized forms by human endothelial cells. Biochim Biophys Acta, 1640(2-3), 153-61, 2003

May, JM, Qu, ZC, Whitesell, RR. Generation of oxidant stress in cultured endothelial cells by methylene blue: protective effects of glucose and ascorbic acid. Biochem Pharmacol, 66(5), 777-84, 2003

Whitesell, RR, Ardehali, H, Printz, RL, Beechem, JM, Knobel, SM, Piston, DW, Granner, DK, Van Der Meer, W, Perriott, LM, May, JM. Control of glucose phosphorylation in L6 myotubes by compartmentalization, hexokinase, and glucose transport. Biochem J, 370(Pt 1), 47-56, 2003 PMCID:1223141

Li, X., Hill, K.E., Burk, R.F., and May, J.M. Selenium spares ascorbate and -tocopherol in cultured liver cell lines under oxidant stress. FEBS Letts., 2001, in press.

Li, X., Qu, Z.-C., and May, J.M. GSH is required to recycle ascorbic acid in cultured liver cell lines, Antiox. Red. Signal., 2001, in press.

May, J.M. (1999) Trans-plasma membrane antioxidant activity of ascorbic acid. FASEB J., in press.

Hardy, TA, May, JM. Coordinate regulation of L-arginine uptake and nitric oxide synthase activity in cultured endothelial cells. Free Radic Biol Med, 32(2), 122-31, 2002

Jones, W, Li, X, Qu, ZC, Perriott, L, Whitesell, RR, May, JM. Uptake, recycling, and antioxidant actions of alpha-lipoic acid in endothelial cells. Free Radic Biol Med, 33(1), 83-93, 2002

Li, X, Cobb, CE, May, JM. Mitochondrial recycling of ascorbic acid from dehydroascorbic acid: dependence on the electron transport chain. Arch Biochem Biophys, 403(1), 103-10, 2002

Li, X, May, JM. Catalase-dependent measurement of H2O2 in intact mitochondria. Mitochondrion, 1(5), 447-53, 2002

May, J.M. Recycling of vitamin C by mammalian thioredoxin reductase. Methods Enzymol., 347, 327-332, 2002

May, JM. Recycling of vitamin C by mammalian thioredoxin reductase. Methods Enzymol, 347, 327-32, 2002

May, JM, Morrow, JD, Burk, RF. Thioredoxin reductase reduces lipid hydroperoxides and spares alpha-tocopherol. Biochem Biophys Res Commun, 292(1), 45-9, 2002

Hill, K.E., Motley, A.K., Li, X., May, J.M., and Burk, R.F. Combined selenium and vitamin E deficiency causes fatal myopathy in guinea pigs. J. Nutr., 131, 1798-1802, 2001

Hill, KE, Motley, AK, Li, X, May, JM, Burk, RF. Combined selenium and vitamin E deficiency causes fatal myopathy in guinea pigs. J Nutr, 131(6), 1798-802, 2001

Lekse, JM, Xia, L, Stark, J, Morrow, JD, May, JM. Plant catechols prevent lipid peroxidation in human plasma and erythrocytes. Mol Cell Biochem, 226(1-2), 89-95, 2001

Li, X, Cobb, CE, Hill, KE, Burk, RF, May, JM. Mitochondrial uptake and recycling of ascorbic acid. Arch Biochem Biophys, 387(1), 143-53, 2001

Li, X, Hill, KE, Burk, RF, May, JM. Selenium spares ascorbate and alpha-tocopherol in cultured liver cell lines under oxidant stress. FEBS Lett, 508(3), 489-92, 2001

Li, X, Qu, ZC, May, JM. GSH is required to recycle ascorbic acid in cultured liver cell lines. Antioxid Redox Signal, 3(6), 1089-97, 2001

Li, X., Cobb, C.E, Hill, K.E., Burk, R.F., and May, J.M. Mitochondrial uptake and recycling of ascorbic acid. Arch. Biochem. Biophys, 387, 143-153, 2001

May, J.M., Qu, Z.-C., and Li, X. Requirement for GSH in recycling of ascorbic acid in endothelial cells. Biochem. Pharmacol, 63, 873-881, 2001

May, J.M., Qu., Z.-C., and Cobb, C.E. Recycling of the ascorbate free radical by human erythrocyte membranes. Free Radic. Biol. Med, 31, 117-124, 2001

May, J.M., Qu., Z.-C., and Morrow, J. Mechanisms of ascorbic acid recycling in human erythrocytes. Biochim.Biophys. Acta, 1528, 159-166, 2001

May, JM, Qu, Z, Cobb, CE. Recycling of the ascorbate free radical by human erythrocyte membranes. Free Radic Biol Med, 31(1), 117-24, 2001

May, JM, Qu, Z, Li, X. Requirement for GSH in recycling of ascorbic acid in endothelial cells. Biochem Pharmacol, 62(7), 873-81, 2001

May, JM, Qu, Z, Morrow, JD. Mechanisms of ascorbic acid recycling in human erythrocytes. Biochim Biophys Acta, 1528(2-3), 159-66, 2001

Perriott, L., Kono, T., Whitesell, R.R., Knobel, S., Piston, D.W., Granner, D.K., Powers, A.C., and May, J.M. Glucose uptake and metabolism by cultured human skeletal muscle cells: rate-limiting steps. Am. J. Physiol, 281, E72-E80, 2001

Perriott, LM, Kono, T, Whitesell, RR, Knobel, SM, Piston, DW, Granner, DK, Powers, AC, May, JM. Glucose uptake and metabolism by cultured human skeletal muscle cells: rate-limiting steps. Am J Physiol Endocrinol Metab, 281(1), E72-80, 2001

May, J.M. How does ascorbic acid prevent endothelial dysfunction?. Free Radic. Biol. Med, 28, 1421-1429, 2000

May, J.M., Qu, Z.-C., Li, X., and Cobb, C.E. Nitrite uptake and metabolism and oxidant stress in human erythrocytes. Amer. J. Physiol., 279, C1946-C1954, 2000

May, J.M., Qu, Z.-C., Morrow, J.D., and Cobb, C.E. Ascorbate-dependent protection of human erythrocytes against oxidant stress generated by diazobenzene sulfonate. Biochem. Pharmacol., 60, 47-53, 2000

May, J.M., Qu, Z.-C., and Cobb, C. Extracellular reduction of the ascorbate free radical by human erythrocytes. Biochem. Biophys. Res. Commun, 267, 118-123, 2000

May, JM. How does ascorbic acid prevent endothelial dysfunction. Free Radic Biol Med, 28(9), 1421-9, 2000

May, JM, Qu, Z, Cobb, CE. Extracellular reduction of the ascorbate free radical by human erythrocytes. Biochem Biophys Res Commun, 267(1), 118-23, 2000

May, JM, Qu, Z, Morrow, JD, Cobb, CE. Ascorbate-dependent protection of human erythrocytes against oxidant stress generated by extracellular diazobenzene sulfonate. Biochem Pharmacol, 60(1), 47-53, 2000

May, JM, Qu, ZC. Troglitazone protects human erythrocytes from oxidant damage. Antioxid Redox Signal, 2(2), 243-50, 2000

May, JM, Qu, ZC, Xia, L, Cobb, CE. Nitrite uptake and metabolism and oxidant stress in human erythrocytes. Am J Physiol Cell Physiol, 279(6), C1946-54, 2000

May, J.M.. Ascorbate-dependent electron transfer across the human erythrocyte membrane. Biochim. Biophys. Acta, 1421, 19-31, 1999

Ardehali, H, Printz, RL, Whitesell, RR, May, JM, Granner, DK. Functional interaction between the N- and C-terminal halves of human hexokinase II. J Biol Chem, 274(23), 15986-9, 1999

May, J.M., Mendiratta, S., Qu, Z.-C. Role of ascorbic acid in transferrin-independent reduction and uptake of iron by U-937 cells. Biochem.Pharmacol., 57, 1275-1282, 1999

May, J.M., Mendiratta, S., Qu, Z.-C., and Loggins, E. Vitamin C recycling and function in human monocytic U-937 cells. Free Radic. Biol. Med, 26, 1513-1523, 1999

May, JM. Is ascorbic acid an antioxidant for the plasma membrane. FASEB J, 13(9), 995-1006, 1999

May, JM, Mendiratta, S, Qu, ZC, Loggins, E. Vitamin C recycling and function in human monocytic U-937 cells. Free Radic Biol Med, 26(11-12), 1513-23, 1999

May, JM, Qu, ZC. Ascorbate-dependent electron transfer across the human erythrocyte membrane. Biochim Biophys Acta, 1421(1), 19-31, 1999

May, JM, Qu, ZC, Mendiratta, S. Role of ascorbic acid in transferrin-independent reduction and uptake of iron by U-937 cells. Biochem Pharmacol, 57(11), 1275-82, 1999

Mitsuyama, H, May, JM. Uptake and antioxidant effects of ergothioneine in human erythrocytes. Clin Sci (Lond), 97(4), 407-11, 1999

Mitsuyama, H., and May, J.M. Uptake and antioxidant effects of ergothioneine in human erythrocytes. Clin. Sci., 97, 407-411, 1999

Ross, D, Mendiratta, S, Qu, ZC, Cobb, CE, May, JM. Ascorbate 6-palmitate protects human erythrocytes from oxidative damage. Free Radic Biol Med, 26(1-2), 81-9, 1999

Ross, D., Qu, Z.-C., Cobb, C. E., and May, J.M. Protection of human erythrocytes from oxidation by ascorbate 6-palmitate, a membrane-bound ascorbic acid derivative. Free Radic. Biol. Med., 26, 81-89, 1999

Vincent, TE, Mendiratta, S, May, JM. Inhibition of aldose reductase in human erythrocytes by vitamin C. Diabetes Res Clin Pract, 43(1), 1-8, 1999

Davis, J. L., Jr., Mendiratta, S., and May, J.M.. Alloxan metabolism mimics that of dehydroascorbate in human erythrocytes. Biochem. Pharmacol., 55, 1301-1307, 1998

Davis, JL, Mendiratta, S, May, JM. Similarities in the metabolism of alloxan and dehydroascorbate in human erythrocytes. Biochem Pharmacol, 55(8), 1301-7, 1998

May, J. M. Ascorbate function and metabolism in the human erythrocyte. Front. Biosci., 2, d1-d10, 1998

May, J.M., Cobb, C.E., Mendiratta, S., Hill, K.E., and R.F. Burk. Reduction of the ascorbyl free radical to ascorbate by thioredoxin reductase. J. Biol. Chem, 273, 23039- 23045, 1998

May, J.M., Qu, Z.-C.., and A. Mendiratta. Protection and recycling of a-tocopherol in human erythrocytes by intracellular ascorbic acid. Arch. Biochem. Biophys, 349, 281-289, 1998

May, JM. Ascorbate function and metabolism in the human erythrocyte. Front Biosci, 3, d1-10, 1998

May, JM, Cobb, CE, Mendiratta, S, Hill, KE, Burk, RF. Reduction of the ascorbyl free radical to ascorbate by thioredoxin reductase. J Biol Chem, 273(36), 23039-45, 1998

May, JM, Qu, ZC, Mendiratta, S. Protection and recycling of alpha-tocopherol in human erythrocytes by intracellular ascorbic acid. Arch Biochem Biophys, 349(2), 281-9, 1998

Mendiratta, S, Qu, Z, May, JM. Erythrocyte defenses against hydrogen peroxide: the role of ascorbic acid. Biochim Biophys Acta, 1380(3), 389-95, 1998

Mendiratta, S, Qu, ZC, May, JM. Enzyme-dependent ascorbate recycling in human erythrocytes: role of thioredoxin reductase. Free Radic Biol Med, 25(2), 221-8, 1998

Mendiratta, S, Qu, ZC, May, JM. Erythrocyte ascorbate recycling: antioxidant effects in blood. Free Radic Biol Med, 24(5), 789-97, 1998

Mendiratta, S., Qu, Z.-C, and May, J.M. Erythrocyte defenses against hydrogen peroxide: The role of ascorbic acid. Biochim. Biophys. Acta, 1380, 389-395, 1998

Mendiratta, S., Qu, Z.-C., and May, J.M. Erythrocyte ascorbate recycling: Antioxidant effects in blood. Free Radic. Bio. Med., 24, 789-797, 1998

Mendiratta, S., Qu, Z.C., and May, J.M. Enzyme-dependent ascorbate recycling in human erythrocytes: role of thioredoxin reductase. Free Radic. Biol. Med., 25, 221-228, 1998

May, J.M., Mendiratta, S., Hill, K.E., and R.F. Burk. Reduction of dehydroascorbate to ascorbate by the selenoenzyme thioredoxin reductase. J. Biol. Chem, 272, 22607-22610, 1997

May, JM, Mendiratta, S, Hill, KE, Burk, RF. Reduction of dehydroascorbate to ascorbate by the selenoenzyme thioredoxin reductase. J Biol Chem, 272(36), 22607-10, 1997

Waters, R.E. II, White, L.L., and May, J.M. Liposomes containing alpha-tocopherol and ascorbate are protected from an external oxidant stress. Free Radical Res, 26, 373-379, 1997

Waters, RE, White, LL, May, JM. Liposomes containing alpha-tocopherol and ascorbate are protected from an external oxidant stress. Free Radic Res, 26(4), 373-9, 1997

Ardehali, H, Yano, Y, Printz, RL, Koch, S, Whitesell, RR, May, JM, Granner, DK. Functional organization of mammalian hexokinase II. Retention of catalytic and regulatory functions in both the NH2- and COOH-terminal halves. J Biol Chem, 271(4), 1849-52, 1996

Ardehali, H., Yano, Y., Printz, R.L., Koch, S., Whitesell, R.R., May, J.M., and D.K. Granner. Functional organization of mammalian hexokinase II. Retention of catalytic and regulatory functions in both the NH2- and COOH-terminal halves. J. Biol.Chem., 271, 1849-1852, 1996

May, J.M., Qu, Z.-C., Whitesell, R.R., and Cobb, C.E. Accessibility and reactivity of ascorbate 6_palmitate bound to human erythrocytes. Free Rad. Biol. Med, 21, 471-480, 1996

May, J.M., Qu, Z.C., and Morrow, J.D. . Interaction of ascorbate and alpha-tocopherol in resealed erythrocyte ghosts - Transmembrane electron transfer and protection from lipid peroxidation. J.Biol.Chem, 271, 10577-10582, 1996

May, J.M., Zhi-chao, Q., Whitesell, R.R., and Cobb, C.E. Ascorbate recycling in human erythrocytes: role of GSH in reducing dehydroascorbate. Free Rad. Biol. Med., 20, 543-551, 1996

May, JM, Qu, ZC, Cobb, CE. Accessibility and reactivity of ascorbate 6-palmitate bound to erythrocyte membranes. Free Radic Biol Med, 21(4), 471-80, 1996

May, JM, Qu, ZC, Morrow, JD. Interaction of ascorbate and alpha-tocopherol in resealed human erythrocyte ghosts. Transmembrane electron transfer and protection from lipid peroxidation. J Biol Chem, 271(18), 10577-82, 1996

May, JM, Qu, ZC, Whitesell, RR, Cobb, CE. Ascorbate recycling in human erythrocytes: role of GSH in reducing dehydroascorbate. Free Radic Biol Med, 20(4), 543-51, 1996

Due, A.D., Zhi-chao, Q., Powers, A.C. and May, J.M. The role of the C-terminal tail of the GLUT1 glucose transporter in its expression and function in Xenopus laevis oocytes. Biochemistry, 34, 5462-5471, 1995

Due, AD, Cook, JA, Fletcher, SJ, Qu, ZC, Powers, AC, May, JM. A "cysteineless" GLUT1 glucose transporter has normal function when expressed in Xenopus oocytes. Biochem Biophys Res Commun, 208(2), 590-6, 1995

Due, AD, Qu, ZC, Thomas, JM, Buchs, A, Powers, AC, May, JM. Role of the C-terminal tail of the GLUT1 glucose transporter in its expression and function in Xenopus laevis oocytes. Biochemistry, 34(16), 5462-71, 1995

May, J.M., Zhi-chao, Q. and Whitesell, R.R. Ascorbate is the major electron donor for a transmembrane oxidoreductase of human erythrocytes. Biochim. Biophys. Acta, 1238, 127-136, 1995

May, J.M., Zhi-chao, Q. and Whitesell, R.R. Ascorbic acid recycling enhances the antioxidant reserve of human erythrocytes. Biochemistry, 34, 12721-12728, 1995

May, JM, Qu, ZC, Whitesell, RR. Ascorbate is the major electron donor for a transmembrane oxidoreductase of human erythrocytes. Biochim Biophys Acta, 1238(2), 127-36, 1995

May, JM, Qu, ZC, Whitesell, RR. Ascorbic acid recycling enhances the antioxidant reserve of human erythrocytes. Biochemistry, 34(39), 12721-8, 1995

Whitesell, RR, Ward, M, McCall, AL, Granner, DK, May, JM. Coupled glucose transport and metabolism in cultured neuronal cells: determination of the rate-limiting step. J Cereb Blood Flow Metab, 15(5), 814-26, 1995

Morita, H, Yano, Y, Niswender, KD, May, JM, Whitesell, RR, Wu, L, Printz, RL, Granner, DK, Magnuson, MA, Powers, AC. Coexpression of glucose transporters and glucokinase in Xenopus oocytes indicates that both glucose transport and phosphorylation determine glucose utilization. J Clin Invest, 94(4), 1373-82, 1994 PMCID:295259

May, J.M. and Beechem, J.M.. Monitoring conformational change in the human erythrocyte glucose carrier: use of a fluorescent probe attached to an exofacial carrier sulfhydryl. Biochemistry, 32, 2907-2915, 1993

May, J.M., Zhi-Chao, Q., and Beechem, J.M.. Tryptic digestion of the human erythrocyte glucose transporter: effects on ligand binding and tryptophan fluorescence. Biochemistry, 32, 9524-9531, 1993

Feman, SS, Mericle, RA, Reed, GW, May, JM, Workman, RJ. Serum angiotensin converting enzyme in diabetic patients. Am J Med Sci, 305(5), 280-4, 1993

May, JM, Beechem, JM. Monitoring conformational change in the human erythrocyte glucose carrier: use of a fluorescent probe attached to an exofacial carrier sulfhydryl. Biochemistry, 32(11), 2907-15, 1993

May, JM, Qu, ZC, Beechem, JM. Tryptic digestion of the human erythrocyte glucose transporter: effects on ligand binding and tryptophan fluorescence. Biochemistry, 32(37), 9524-31, 1993

Whitesell, RR, Aboumrad, MK, Powers, AC, Regen, DM, Le, C, Beechem, JM, May, JM, Abumrad, NA. Coupling of glucose transport and phosphorylation in Xenopus oocytes and cultured cells: determination of the rate-limiting step. J Cell Physiol, 157(3), 509-18, 1993

Yano, Y, May, JM. Ligand-induced conformational changes modify proteolytic cleavage of the adipocyte insulin-sensitive glucose transporter. Biochem J, 295 ( Pt 1), 183-8, 1993 PMCID:1134836

May, JM. The one-site model of human erythrocyte glucose transport: testing its predictions using network thermodynamic computer simulations. Biochim Biophys Acta, 1064(1), 1-6, 1991

May, JM. Thiol-fatty acylation of the glucose transport protein of human erythrocytes. FEBS Lett, 274(1-2), 119-21, 1990

May, JM, Buchs, A, Carter-Su, C. Localization of a reactive exofacial sulfhydryl on the glucose carrier of human erythrocytes. Biochemistry, 29(45), 10393-8, 1990

May, JM. Differential labeling of the erythrocyte hexose carrier by N-ethylmaleimide: correlation of transport inhibition with reactive carrier sulfhydryl groups. Biochim Biophys Acta, 986(2), 207-16, 1989

May, JM. Interaction of a permeant maleimide derivative of cysteine with the erythrocyte glucose carrier. Differential labelling of an exofacial carrier thiol group and its role in the transport mechanism. Biochem J, 263(3), 875-81, 1989 PMCID:1133512

May, JM. Inhibition of hexose transport in the human erythrocyte by 5, 5''-dithiobis(2-nitrobenzoic acid): role of an exofacial carrier sulfhydryl group. J Membr Biol, 108(3), 227-33, 1989

May, JM. Selective labeling of the erythrocyte hexose carrier with a maleimide derivative of glucosamine: relationship of an exofacial sulfhydryl to carrier conformation and structure. Biochemistry, 28(4), 1718-25, 1989

May, JM. Inhibition of hexose transport and labelling of the hexose carrier in human erythrocytes by an impermeant maleimide derivative of maltose. Biochem J, 254(2), 329-36, 1988 PMCID:1135081

May, JM. Inhibition of hexose transport by adenosine derivatives in human erythrocytes. J Cell Physiol, 135(2), 332-8, 1988

May, JM. Effects of ATP depletion on the mechanism of hexose transport in intact human erythrocytes. FEBS Lett, 241(1-2), 188-90, 1988

May, JM. Reaction of an exofacial sulfhydryl group on the erythrocyte hexose carrier with an impermeant maleimide. Relevance to the mechanism of hexose transport. J Biol Chem, 263(27), 13635-40, 1988

May, JM, Danzo, BJ. Photolabeling of the human erythrocyte glucose carrier with androgenic steroids. Biochim Biophys Acta, 943(2), 199-210, 1988


Postdoctoral Position Available
no

Postdoctoral Position Details
N/A

Updated Date
11/20/2013