Biomedical Research Education & Training
Faculty Member

Eichman, Brandt F., Ph.D.
Associate Professor of Biological Sciences
Associate Professor of Biochemistry

Lab Url: http://structbio.vanderbilt.edu/eichman

Phone Number: (615) 936-5233

Email Address: brandt.eichman@vanderbilt.edu

Eichman, Brandt's picture
Academic history
B.S., University of Mississippi
Ph.D., Oregon State University
Postdoc, Harvard Medical School

Office Address   Mailing Address

MRBIII 5270A / 5274

Department of Biological Sciences, Vanderbilt University Box 351634, Station B 37235-1634


Research Keywords
DNA replication, DNA repair, protein-DNA interactions, structural biology, X-ray crystallography, enzymology, biochemistry

Research Specialty
Structural biology of DNA replication and DNA repair; X-ray crystallography

Research Description
DNA damage arising from exposure to environmental toxins and cellular metabolites thwarts DNA replication and leads to genome instability, cell death, and diseases including cancer. Our laboratory uses the tools of structural biology and biochemistry to investigate molecular mechanisms of proteins involved in repairing DNA damage and maintaining replication fork progression. We primarily use X-ray crystallography as a starting point to understand how these proteins recognize and manipulate DNA structure to carry out their particular functions. Current work focuses on base excision repair of DNA alkylation damage by DNA glycosylases, repair of stalled replication forks by structure-specific DNA translocases, and the coordination of DNA unwinding and synthesis during eukaryotic replication. The long-term goals are to understand the fundamental processes underlying genome maintenance and to develop new therapeutic strategies that target genetic diseases.

Publications
Brooks, SC, Fischer, RL, Huh, JH, Eichman, BF. 5-Methylcytosine Recognition by Arabidopsis thaliana DNA Glycosylases DEMETER and DML3. Biochemistry, 2014

Jang, H, Shin, H, Eichman, BF, Huh, JH. Excision of 5-hydroxymethylcytosine by DEMETER family DNA glycosylases. Biochem Biophys Res Commun, 2014

Mullins, EA, Rubinson, EH, Eichman, BF. The substrate binding interface of alkylpurine DNA glycosylase AlkD. DNA Repair (Amst), 13, 50-54, 2014

Troll, CJ, Adhikary, S, Cueff, M, Mitra, I, Eichman, BF, Camps, M. Interplay between base excision repair activity and toxicity of 3-methyladenine DNA glycosylases in an?? E. coli complementation system. Mutat Res, 2014

Adhikary, S, Cato, MC, McGary, KL, Rokas, A, Eichman, BF. Non-productive DNA damage binding by DNA glycosylase-like protein Mag2 from Schizosaccharomyces pombe. DNA Repair (Amst), 12, 196-204, 2013

Betous, R, Couch, FB, Mason, AC, Eichman, BF, Manosas, M, Cortez, D. Substrate-Selective Repair and Restart of Replication Forks by DNA Translocases. Cell Rep, 3, 1-12, 2013

Brooks, SC, Adhikary, S, Rubinson, EH, Eichman, BF. Recent advances in the structural mechanisms of DNA glycosylases. Biochim Biophys Acta, 1834(1), 247-71, 2013

Du, W, Josephrajan, A, Adhikary, S, Bowles, T, Bielinsky, AK, Eichman, BF. Mcm10 self-association is mediated by an N-terminal coiled-coil domain. PLoS One, 8(7), e70518, 2013

Mullins, EA, Rubinson, EH, Pereira, KN, Wade Calcutt, M, Christov, PP, Eichman, BF. An HPLC-tandem mass spectrometry method for simultaneous detection of alkylated base excision repair products. Methods, 64(1), 59-66, 2013

Rubinson, EH, Christov, PP, Eichman, BF. Depurination of N7-Methylguanine by DNA Glycosylase AlkD Is Dependent on the DNA Backbone. Biochemistry, 52(42), 7363-5, 2013

Vaithiyalingam, S, Arnett, DR, Aggarwal, A, Eichman, BF, Fanning, E, Chazin, WJ. Insights into Eukaryotic Primer Synthesis from Structures of the p48 Subunit of Human DNA Primase. J Mol Biol, 2013

Betous, R, Mason, AC, Rambo, RP, Bansbach, CE, Badu-Nkansah, A, Sirbu, BM, Eichman, BF, Cortez, D. SMARCAL1 catalyzes fork regression and Holliday junction migration to maintain genome stability during DNA replication. Genes Dev, 26(2), 151-62, 2012 PMCID:3273839

Du, W, Stauffer, ME, Eichman, BF. Structural biology of replication initiation factor Mcm10. Subcell Biochem, 62, 197-216, 2012

Rubinson, EH, Eichman, BF. Nucleic acid recognition by tandem helical repeats. Curr Opin Struct Biol, 22(1), 101-9, 2012

Shi, M, Pedchenko, V, Greer, BH, Van Horn, WD, Santoro, SA, Sanders, CR, Hudson, BG, Eichman, BF, Zent, R, Pozzi, A. Enhancing integrin alpha1 I-domain affinity to ligand potentiates integrin alpha1beta1-mediated downregulation of collagen synthesis. J Biol Chem, 287, 35139-35152, 2012

Adhikary, S, Eichman, BF. Analysis of substrate specificity of Schizosaccharomyces pombe Mag1 alkylpurine DNA glycosylase. EMBO Rep, 12(12), 1286-92, 2011

Camps, M, Eichman, BF. Unraveling a connection between DNA demethylation repair and cancer. Mol Cell, 44(3), 343-4, 2011

Mok, YG, Uzawa, R, Lee, J, Weiner, GM, Eichman, BF, Fischer, RL, Huh, JH. Domain structure of the DEMETER 5-methylcytosine DNA glycosylase. Proc Natl Acad Sci U S A, 107(45), 19225-30, 2010 PMCID:2984145

Robertson, PD, Chagot, B, Chazin, WJ, Eichman, BF. Solution NMR structure of the C-terminal DNA binding domain of Mcm10 reveals a conserved MCM motif. J Biol Chem, 285(30), 22942-9, 2010

Rubinson, EH, Adhikary, S, and Eichman, BF. Structural Studies of Alkylpurine DNA Glycosylases. ACS Symposium Series : Structural Biology of DNA Damage and Repair., 1041, 29-45, 2010

Rubinson, EH, Gowda, AS, Spratt, TE, Gold, B, Eichman, BF. An unprecedented nucleic acid capture mechanism for excision of DNA damage. Nature, 468(7322), 406-11, 2010

Vaithiyalingam, S, Warren, EM, Eichman, BF, Chazin, WJ. Insights into eukaryotic DNA priming from the structure and functional interactions of the 4Fe-4S cluster domain of human DNA primase. Proc Natl Acad Sci U S A, 107(31), 13684-9, 2010

Warren, EM, Huang, H, Fanning, E, Chazin, WJ, Eichman, BF. Physical interactions between MCM10, DNA, AND DNA polymerase alpha. J Biol Chem, 284(36), 24662-24672, 2009

Bowles, T*, Metz, AH*, O'Quin, J, Wawrzak, Z, Eichman, BF. Structure and DNA binding of alkylation response protein AidB. Proc Natl Acad Sci U S A, 105(40), 15299-15304, 2008 PMCID:2563087

Robertson, PD*, Warren, EM*, Zhang, H*, Friedman, DB, Lary, JW, Cole, JL, Tutter, AV, Walter, JC, Fanning, E, Eichman, BF. Domain architecture and biochemical characterization of vertebrate Mcm10. J Biol Chem, 283(6), 3338-48, 2008 PMCID:2753450

Rubinson, EH, Metz, AH, O'Quin, J, Eichman, BF. A new protein architecture for processing alkylation damaged DNA: the crystal structure of DNA glycosylase AlkD. J Mol Biol, 381(1), 13-23, 2008

Warren, EM, Vaithiyalingam, S, Haworth, J, Greer, B, Bielinsky, AK, Chazin, WJ, Eichman, BF. Structural basis for DNA binding by replication initiator mcm10. Structure, 16(12), 1892-901, 2008 PMCID:2636851

Metz, AH, Hollis, T, Eichman, BF. DNA damage recognition and repair by 3-methyladenine DNA glycosylase I (TAG). EMBO J, 26(9), 2411-20, 2007 PMCID:1864966

Brieba, Luis G, Eichman, Brandt F, Kokoska, Robert J, Doublie, Sylvie, Kunkel, Tom A, Ellenberger, Tom. Structural basis for the dual coding potential of 8-oxoguanosine by a high-fidelity DNA polymerase. EMBO J, 23(17), 3452-3461, 2004 PMCID:516626

Eichman BF, Fanning E. The power of pumping together; deconstructing the engine of a DNA replication machine. Cell, 119(1), 3-4, 2004

Eichman, BF, Fanning, E. The power of pumping together; deconstructing the engine of a DNA replication machine. Cell, 119(1), 3-4, 2004 PMCID:1864966

Eichman, Brandt F, O''Rourke, Eyleen J, Radicella, J Pablo, Ellenberger, Tom. Crystal structures of 3-methyladenine DNA glycosylase MagIII and the recognition of alkylated bases. EMBO J, 22(19), 4898-909, 2003 PMCID:204488

Eichman, Brandt F, Ortiz-Lombardia, Miguel, Aymami, Joan, Coll, Miquel, Ho, Pui Shing. The inherent properties of DNA four-way junctions: comparing the crystal structures of holliday junctions. J Mol Biol, 320(5), 1037-51, 2002

Eichman, B F, Mooers, B H, Alberti, M, Hearst, J E, Ho, P S. The crystal structures of psoralen cross-linked DNAs: drug-dependent formation of Holliday junctions. J Mol Biol, 308(1), 15-26, 2001

Ho, P S, Eichman, B F. The crystal structures of DNA Holliday junctions. Curr Opin Struct Biol, 11(3), 302-8, 2001

Eichman, B F, Vargason, J M, Mooers, B H, Ho, P S. The Holliday junction in an inverted repeat DNA sequence: sequence effects on the structure of four-way junctions. Proc Natl Acad Sci U S A, 97(8), 3971-6, 2000 PMCID:18126

Vargason, J M, Eichman, B F, Ho, P S. The extended and eccentric E-DNA structure induced by cytosine methylation or bromination. Nat Struct Biol, 7(9), 758-61, 2000

Basham, B, Eichman, BF, and Ho, PS. The single crystal structures of Z-DNA. In Neidle, S (ed.), The Oxford Handbook of Nucleic Acid Structure. Oxford University Press, Oxford, UK, 1, 199-252, 1999

Eichman, B F, Schroth, G P, Basham, B E, Ho, P S. The intrinsic structure and stability of out-of-alternation base pairs in Z-DNA. Nucleic Acids Res, 27(2), 543-50, 1999 PMCID:148213

Mooers, B H, Eichman, B F, Ho, P S. The structures and relative stabilities of d(G x G) reverse Hoogsteen, d(G x T) reverse wobble, and d(G x C) reverse Watson-Crick base-pairs in DNA crystals. J Mol Biol, 269(5), 796-810, 1997


Postdoctoral Position Available
Yes

Postdoctoral Position Details
A postdoctoral position is available to study the structures and functions of proteins involved in i) initiation of eukaryotic DNA replication and ii) recognition and repair of damaged DNA. The PI?s laboratory uses X-ray crystallography and other structural biology, biochemical, and biophysical methods to investigate the structure-function relationship of DNA processing enzymes and their macromolecular complexes. Experience in macromolecular X-ray crystallography is preferred, but not required. Applicants should send a cover letter, CV, and names of 3 references to brandt.eichman@vanderbilt.edu.

Updated Date
01/10/2014