Biomedical Research Education & Training
Faculty Member

Zaika, Alexander, Ph.D.
Associate Professor of Surgery
Assistant Professor of Cancer Biology

Lab Url: http://Department of Surgery

Phone Number: (615)322-7209

Email Address: alex.zaika@vanderbilt.edu

Zaika, Alexander's picture
Academic history
Ph.D., University of Chemical Technology, Moscow, Russia
M.S., Petersburg State University, Saint Petersburg, Russia
B.S., Petersburg State University, Saint Petersburg, Russia

Office Address   Mailing Address

1255 Light Hall

Vanderbilt University Medical Center, 1255 Light Hall 37232


Research Keywords
Cancer Research,Tumor Suppressors,Oncogenes,p53 protein family,Apoptosis,Cancer,Cell cycle,Gene regulation,Malignancy,Signal transduction,Transcription,Transcription factor

Research Specialty
Molecular Mechanisms of Tumorigenesis, p53 protein family

Research Description
p53 protein family, which consists of p53, p63 and p73, plays a pivotal role in regulation of multiple cellular mechanisms including apoptosis, cell cycle control and DNA damage repair. We are interested in understanding the molecular and cellular machinery that underlie the functions of these proteins. Although the role of p53 in human studies is relatively well studied, less is known about p73 and p63. Our analyses demonstrated that p73 and p63 family interact with each-other and these interactions play a critical role in human tumorigenesis. Our data also suggest that these proteins are strongly involved in a response to anticancer curative treatment. We are using a number of different in vitro and in vivo techniques to examine the role of the p53 protein family in cancer development and progression.



Publications
Vilgelm, A, Wei, JX, Piazuelo, MB, Washington, MK, Prassolov, V, El-Rifai, W, Zaika, A. DeltaNp73alpha regulates MDR1 expression by inhibiting p53 function. Oncogene, 27(15), 2170-6, 2008

Wei, J, O''Brien, D, Vilgelm, A, Piazuelo, MB, Correa, P, Washington, MK, El-Rifai, W, Peek, RM, Zaika, A. Interaction of Helicobacter pylori with gastric epithelial cells is mediated by the p53 protein family. Gastroenterology, 134(5), 1412-23, 2008 PMCID:2430883

Tomkova, K, El-Rifai, W, Vilgelm, A, Kelly, MC, Wang, TC, Zaika, AI. The gastrin gene promoter is regulated by p73 isoforms in tumor cells. Oncogene, 2006

Zaika, AI, El-Rifai, W. The role of p53 protein family in gastrointestinal malignancies. Cell Death Differ, 13(6), 935-40, 2006

Belkhiri, A, Zaika, A, Pidkovka, N, Knuutila, S, Moskaluk, C, El-Rifai, W. Darpp-32: a novel antiapoptotic gene in upper gastrointestinal carcinomas. Cancer Res, 65(15), 6583-92, 2005

Koon, N, Zaika, A, Moskaluk, CA, Frierson, HF, Knuutila, S, Powell, SM, El-Rifai, W. Clustering of molecular alterations in gastroesophageal carcinomas. Neoplasia, 6(2), 143-9, 2004 PMCID:1502088

Slade, N, Zaika, AI, Erster, S, Moll, UM. DeltaNp73 stabilises TAp73 proteins but compromises their function due to inhibitory hetero-oligomer formation. Cell Death Differ, 11(3), 357-60, 2004

Tomkova, K, Belkhiri, A, El-Rifai, W, Zaika, AI. p73 isoforms can induce T-cell factor-dependent transcription in gastrointestinal cells. Cancer Res, 64(18), 6390-3, 2004

Varis, A, Zaika, A, Puolakkainen, P, Nagy, B, Madrigal, I, Kokkola, A, V??yrynen, A, K??rkk??inen, P, Moskaluk, C, El-Rifai, W, Knuutila, S. Coamplified and overexpressed genes at ERBB2 locus in gastric cancer. Int J Cancer, 109(4), 548-53, 2004

Joseph, TW, Zaika, A, Moll, UM. Nuclear and cytoplasmic degradation of endogenous p53 and HDM2 occurs during down-regulation of the p53 response after multiple types of DNA damage. FASEB J, 17(12), 1622-30, 2003

Mihara, M, Erster, S, Zaika, A, Petrenko, O, Chittenden, T, Pancoska, P, Moll, UM. p53 has a direct apoptogenic role at the mitochondria. Mol Cell, 11(3), 577-90, 2003

Petrenko, O, Zaika, A, Moll, UM. deltaNp73 facilitates cell immortalization and cooperates with oncogenic Ras in cellular transformation in vivo. Mol Cell Biol, 23(16), 5540-55, 2003 PMCID:166317

Shirangi, TR, Zaika, A, Moll, UM. Nuclear degradation of p53 occurs during down-regulation of the p53 response after DNA damage. FASEB J, 16(3), 420-2, 2002

Zaika, AI, Slade, N, Erster, SH, Sansome, C, Joseph, TW, Pearl, M, Chalas, E, Moll, UM. DeltaNp73, a dominant-negative inhibitor of wild-type p53 and TAp73, is up-regulated in human tumors. J Exp Med, 196(6), 765-80, 2002 PMCID:2194062

Moll, UM, Erster, S, Zaika, A. p53, p63 and p73--solos, alliances and feuds among family members. Biochim Biophys Acta, 1552(2), 47-59, 2001

Moll, UM, Zaika, A. Nuclear and mitochondrial apoptotic pathways of p53. FEBS Lett, 493(2-3), 65-9, 2001

Sansome, C, Zaika, A, Marchenko, ND, Moll, UM. Hypoxia death stimulus induces translocation of p53 protein to mitochondria. Detection by immunofluorescence on whole cells. FEBS Lett, 488(3), 110-5, 2001

Zaika, A, Irwin, M, Sansome, C, Moll, UM. Oncogenes induce and activate endogenous p73 protein. J Biol Chem, 276(14), 11310-6, 2001

Kovalev, S, Mateen, A, Zaika, AI, O''Hea, BJ, Moll, UM. Lack of defective expression of the ATM gene in sporadic breast cancer tissues and cell lines. Int J Oncol, 16(4), 825-31, 2000

Marchenko, ND, Zaika, A, Moll, UM. Death signal-induced localization of p53 protein to mitochondria. A potential role in apoptotic signaling. J Biol Chem, 275(21), 16202-12, 2000

Moll, UM, Zaika, A. Disrupting the p53-mdm2 interaction as a potential therapeutic modality. Drug Resist Updat, 3(4), 217-221, 2000

Zaika, A, Marchenko, N, Moll, UM. Cytoplasmically "sequestered" wild type p53 protein is resistant to Mdm2-mediated degradation. J Biol Chem, 274(39), 27474-80, 1999

Zaika, A, Mozzherin, DJ, Tan, CK, Downey, KM, Fisher, PA. A two-dimensional support for selective binding of polyhistidine-tagged proteins: identification of a proliferating cell nuclear antigen point mutant with altered function in vitro. Anal Biochem, 268(2), 193-200, 1999

Zaika, AI, Kovalev, S, Marchenko, ND, Moll, UM. Overexpression of the wild type p73 gene in breast cancer tissues and cell lines. Cancer Res, 59(13), 3257-63, 1999


Postdoctoral Position Available
Yes

Postdoctoral Position Details
Postdoctoral and Research Assistant positions are available. Individuals who have strong experience in cellular and molecular biology techniques and interest in cancer science are encouraged to apply.

Updated Date
12/11/2012