Biomedical Research Education & Training
Faculty Member

De Jonge, Niels, PhD
Adjoint Assistant Professor of Molecular Physiology and Biophysics

Lab Url: http://www.mc.vanderbilt.edu/labs/dejongelab/

Phone Number: +49-681-9300-313

Email Address: niels.de.jonge (at) vanderbilt.edu

De Jonge, Niels's picture
Academic history
BS, University of Amsterdam, Netherland
MS, University of Amsterdam, Netherland
PhD, University of Freiburg, Germany
Post-doc, University of Freiburg, Germany

Office Address   Mailing Address

INM-Leibniz Institute for new Materials, 66386


Research Keywords
Molecular-level imaging, electron microscopy, biophysics, cell biology, protein function, nanobiotechnology, nanotechnology

Research Specialty
Electron microscopy of cells

Research Description
Molecular-level imaging with new electron microscopy techniques for biomedical research. This research program aims at developing new scanning transmission electron microscopy (STEM) techniques to investigate the molecular interaction underlying cellular function. Electron microscopy presently has its strength in imaging with high spatial resolution. New phenomena may be discovered by improving the capabilities of time-resolved and three-dimensional (3D) imaging under native (liquid) conditions. The program includes three research projects:

Liquid STEM. Research on scanning transmission electron microscopy (STEM) of whole eukaryotic cells in liquid using a flow cell with electron transparent windows. Nanometer resolution was achieved on specific labels. Liquid STEM is also used in correlative light- and electron microscopy, and can be used for in situ experiments in materials science.
See also: http://www.vanderbilt.edu/exploration/stories/liquidstem.html

3D STEM. Research on 3-dimensional aberration corrected STEM of biological samples based on the principles of confocal microscopy. 3D STEM is an alternative for electron tomography with potentially improved capabilities.

Electron microscopy is conducted at the INM-Leibniz Institute in Saarbruecken, Germany, using the JEOL ARM 200 F aberration corrected STEM/TEM.

Publications
Dahmen, T, Baudoin, JP, Lupini, AR, K??bel, C, Slusallek, P, de Jonge, N. Combined Scanning Transmission Electron Microscopy Tilt- and Focal Series. Microsc Microanal, 1-13, 2014

Peckys, DB, Dukes, MJ, de Jonge, N. Correlative fluorescence and electron microscopy of quantum dot labeled proteins on whole cells in liquid. Methods Mol Biol, 1117, 527-40, 2014

Peckys, DB, de Jonge, N. Liquid Scanning Transmission Electron Microscopy: Imaging Protein Complexes in their Native Environment in Whole Eukaryotic Cells. Microsc Microanal, 1-20, 2014

Peckys, DB, de Jonge, N. Gold nanoparticle uptake in whole cells in liquid examined by environmental scanning electron microscopy. Microsc Microanal, 20(1), 189-97, 2014

de Jonge, N. Introduction to Special Issue on Electron Microscopy of Specimens in Liquid. Microsc Microanal, 1-2, 2014

Baudoin, JP, Jerome, WG, K??bel, C, de Jonge, N. Whole-Cell Analysis of Low-Density Lipoprotein Uptake by Macrophages Using STEM Tomography. PLoS One, 8(1), e55022, 2013

Baudoin, JP, Jinschek, JR, Boothroyd, CB, Dunin-Borkowski, RE, de Jonge, N. Chromatic aberration-corrected tilt series transmission electron microscopy of nanoparticles in a whole mount macrophage cell. Microsc Microanal, 19(4), 814-20, 2013

Kraus, T, de Jonge, N. Dendritic gold nanowire growth observed in liquid with transmission electron microscopy. Langmuir, 29(26), 8427-32, 2013

Peckys, DB, Baudoin, JP, Eder, M, Werner, U, de Jonge, N. Epidermal growth factor receptor subunit locations determined in hydrated cells with environmental scanning electron microscopy. Sci Rep, 3, 2626, 2013

Ramachandra, R, Demers, H, de Jonge, N. The influence of the sample thickness on the lateral and axial resolution of aberration-corrected scanning transmission electron microscopy. Microsc Microanal, 19(1), 93-101, 2013

de Jonge, N. Proceedings of the Conference on In-Situ and Correlative Electron Microscopy (CISCEM), November 6-7, 2012, Saarbrucken, Germany. Advances in Imaging & Electron Physics, 179, 137-202, 2013

Demers, H, Ramachandra, R, Drouin, D, de Jonge, N. The probe profile and lateral resolution of scanning transmission electron microscopy of thick specimens. Microsc Microanal, 18(3), 582-90, 2012

Heeres, EC, Oosterkamp, TH, de Jonge, N. Size of the localized electron emission sites on a closed multiwalled carbon nanotube. Phys Rev Lett, 108(3), 036804, 2012

Ramachandra, R, de Jonge, N. Optimized deconvolution for maximum axial resolution in three-dimensional aberration-corrected scanning transmission electron microscopy. Microsc Microanal, 18(1), 218-28, 2012

Ring, EA, de Jonge, N. Video-frequency scanning transmission electron microscopy of moving gold nanoparticles in liquid. Micron, 43(11), 1078-84, 2012

Demers, H, Poirier-Demers, N, Couture, AR, Joly, D, Guilmain, M, de Jonge, N, Drouin, D. Three-dimensional electron microscopy simulation with the CASINO Monte Carlo software. Scanning, 33(3), 135-46, 2011

Demers, H, Poirier-Demers, N, Couture, ER, Joly, D, Guilman, M, de Jonge, N, Drouin, D. Three-dimensional electron microscopy simulation with the CASINO Monte Carlo software. Scanning, 33, 135-146, 2011

Dukes, MJ, Ramachandra, R, Baudoin, JP, Gray Jerome, W, de Jonge, N. Three-dimensional locations of gold-labeled proteins in a whole mount eukaryotic cell obtained with 3nm precision using aberration-corrected scanning transmission electron microscopy. J Struct Biol, 174(3), 552-62, 2011

Heeres, EC, Oosterkamp, TH, de Jonge, N. Making carbon nanotube electron sources of defined lengths and with closed caps. Nanotechnology, 22, 235308-1-8, 2011

Klein, KL, Anderson, IM, DE Jonge, N. Transmission electron microscopy with a liquid flow cell. J Microsc, 242, 117-123, 2011

Lupini, AR, de Jonge, N. The three-dimensional point spread function of aberration-corrected scanning transmission electron microscopy. Microsc Microanal, 17(5), 817-26, 2011

Peckys, DB, Mazur, P, Gould, KL, de Jonge, N. Fully hydrated yeast cells imaged with electron microscopy. Biophys J, 100(10), 2522-9, 2011

Peckys, DB, de Jonge, N. Visualizing gold nanoparticle uptake in live cells with liquid scanning transmission electron microscopy. Nano Lett, 11(4), 1733-8, 2011

Ramachandra, R, Demers, H, de Jonge, N. Atomic-resolution scanning transmission electron microscopy through 50-nm-thick silicon nitride membranes. Appl Phys Lett, 98(9), 93109, 2011

Ramachandra, R, Demers, H, de Jonge, N. Atomic resolution through silicon nitride membranes. Appl. Phys. Lett., 98, 93109-1-3, 2011

Ring, EA, Peckys, DB, Dukes, MJ, Baudoin, JP, de Jonge, N. Silicon nitride windows for electron microscopy of whole cells. J Microsc, 243(3), 273-83, 2011

de Jonge, N, Ross, FM. Electron microscopy of specimens in liquid. Nat Nanotechnol, 6(11), 695-704, 2011

Demers, H, Poirier-Demers, N, Drouin, D, de Jonge, N. Simulating STEM imaging of nanoparticles in micrometers-thick substrates. Microsc Microanal, 16(6), 795-804, 2010

Dukes, MJ, Peckys, DB, de Jonge, N. Correlative Fluorescence Microscopy and Scanning Transmission Electron Microscopy of Quantum-Dot-Labeled Proteins in Whole Cells in Liquid. ACS Nano, 4, 4110-4116, 2010

Jarvis, JD, Andrews, HL, Ivanov, B, Stewart, CL, de Jonge, N, Heeres, EC, Kang, WP, Wong, YM, Davidson, JL, Brau, CA. Resonant tunneling and extreme brightness from diamond field emitters and carbon nanotubes. J. Appl. Phys., 108, 94322-1-6, 2010

Ring, EA, de Jonge, N. Microfluidic System for Transmission Electron Microscopy. Microsc Microanal, 16, 622-629, 2010

de Jonge, N. Fundamental properties of CNT emitters as an electron source. in: Carbon nanotube and related field emitters: fundamentals and applications, ed. Saito, Y., 2010

de Jonge, N, Bigelow, WC, Veith, GM. Atmospheric pressure scanning transmission electron microscopy. Nano Lett, 10(3), 1028-31, 2010

de Jonge, N, Poirier-Demers, N, Demers, H, Peckys, DB, Drouin, D. Nanometer-resolution electron microscopy through micrometers-thick water layers. Ultramicroscopy, 110, 1114-1119, 2010

de Jonge, N, Sougrat, R, Northan, BM, Pennycook, SJ. Three-dimensional scanning transmission electron microscopy of biological specimens. Microsc Microanal, 16(1), 54-63, 2010

Peckys, DB, Veith, GM, Joy, DC, de Jonge, N. Nanoscale imaging of whole cells using a liquid enclosure and a scanning transmission electron microscope. PLoS One, 4(12), e8214, 2009

de Jonge, N. Carbon nanotube electron sources for electron microscopes. Advances in Imaging & Electron Physics, 156, 2159-2164, 2009

de Jonge, N, Peckys, DB, Kremers, GJ, Piston, DW. Electron microscopy of whole cells in liquid with nanometer resolution. Proc Natl Acad Sci U S A, 106, 2159-2164, 2009 PMCID:2650183

Peckys, DB, de Jonge, N, Simpson, ML, McKnight, TE. End-specific strategies of attachment of long double stranded DNA onto gold-coated nanofiber arrays. Nanotechnology, 19, 435301-10, 2008

Edgcombe, CJ, de Jonge, N. Deduction of work function of carbon nanotube field emitter by use of curved-surface theory. J. Phys. D: Appl. Phys., 40, 4123-4128, 2007

Heeres, EC, Bakkers, EP, Roest, AL, Kaiser, M, Oosterkamp, TH, de Jonge, N. Electron emission from individual indium arsenide semiconductor nanowires. Nano Lett, 7(2), 536-40, 2007

Lupini, AR, Rashkeev, SN, Varela, M, Borisevich, AY, Oxley, MP, van Benthem, K, Peng, Y, de Jonge, N, Veith, GM, Chisholm, MF, Pennycook, SJ. Scanning transmission electron microscopy. in: Nanocharacterization, ed. Kirkland, E.J. & Hutchison, 28-65, 2007

de Jonge, N, Sougrat, R, Peckys, DB, Lupini, AR, Pennycook, SJ. 3-dimensional aberration corrected scanning transmission electron microscopy for biology. in: Nanotechnology in Biology and Medicine, ed. Vo-Dinh, T, 13.1-27, 2007

Doytcheva, M, Kaiser, M, de Jonge, N. In-situ transmission electron microscopy investigation of the structural changes in carbon nanotubes during electron emission at high currents. Nanotechnology, 17, 3226-3233, 2006

Edgcombe, CJ, de Jonge, N. Preparation of a carbon nanotube field emitter and deduction of its properties from curvature the Fowler-Nordheim plot. J. Phys. Conf. S., 26, 315-318, 2006

Edgcombe, CJ, de Jonge, N. Properties of a field emitter deduced from curvature of its Fowler-Nordheim plot. J. Vac. Sci. Technol B, 24, 869-873, 2006

Kaiser, M, Doytcheva, M, Verheijen, M, de Jonge, N. In situ transmission electron microscopy observations of individually selected freestanding carbon nanotubes during field emission. Ultramicroscopy, 106(10), 902-8, 2006

Milne, WI, Teo, KBK, Mann, M, Bu, IYY, Amaratunga, GAJ, de Jonge, N, Allioux, M, Oostveen, JT, Legagneux, P, Minoux, E, Gangloff, L, Hudanski, L, Schnell, JP, Dieumegard, LD, Peauger, F, Wells, T, El-Gomati, M. Carbon nanotubes as electron sources. Phys. Stat. Sol., 203, 1058-1063, 2006

de Jonge, N, Allioux, M, Oostveen, JT, Teo, KB, Milne, WI. Optical performance of carbon-nanotube electron sources. Phys Rev Lett, 94(18), 186807, 2005

de Jonge, N, Allioux, M, Oostveen, JT, Teo, KBK, Milne, WI. Low noise and stable emission from carbon nanotube electron sources. Appl. Phys. Lett., 87, 133118-1-3, 2005

de Jonge, N, Doytcheva, M, Allioux, M, Kaiser, M, Mentink, SAM, Teo, KBK, Lacerda, RG, Milne, WI. Cap closing of thin carbon nanotubes. Adv. Mater., 17, 451-455, 2005

De Jonge, N Allioux, M, Doytcheva, M, Kaiser, M, Teo, KBK, Lacerda, RG, Milne, WI. Characterization of the field emission properties of individual thin carbon nanotubes. Appl. Phys. Lett., 85, 1607-1609, 2004

Doytcheva, M, Kaiser, M, Verheijen, MA, Reyes-Reyes, M, Terrones, M, de Jonge, N. Electron emission from individual nitrogen-doped multi-walled carbon nanotubes. Chem. Phys. Lett., 396, 126-130, 2004

de Jonge, N. The brightness of carbon nanotube electron emitters. J. Appl. Phys., 95, 673-681, 2004

de Jonge, N, Allioux, M, Doytcheva, M, Kaiser, M, Teo, KBK, Lacerda, RG, Milne, WI. Field emission from individual thin carbon nanotubes. AIP Conference Proceedings, N723, 485-489, 2004

de Jonge, N, Bonard, JM. Carbon nanotube electron sources and applications. Philos Transact A Math Phys Eng Sci, 362(1823), 2239-66, 2004

de Jonge, N, Lamy, Y, Kaiser, M. Controlled mounting of individual multi-walled carbon nanotubes on support tips. Nano Lett., 3, 1621-1624, 2003

de Jonge, N, van Druten, NJ. Field emission from individual multiwalled carbon nanotubes prepared in an electron microscope. Ultramicroscopy, 95(1-4), 85-91, 2003

de Jonge, N, Lamy, Y, Schoots, K, Oosterkamp, TH. High brightness electron beam from a multi-walled carbon nanotube. Nature, 420(6914), 393-5, 2002

Rau, HK, DeJonge, N, Haehnel, W. Combinatorial Synthesis of Four-Helix Bundle Hemoproteins for Tuning of Cofactor Properties. Angew Chem Int Ed Engl, 39(1), 250-253, 2000

de Jonge, N, Rau, HK, Haehnel, W. Light induced electron transfer in synthetic metalloproteins. Z. Phys. Chem., 213, 175-180, 1999

Rau, HK, DeJonge, N, Haehnel, W. Modular synthesis of de novo-designed metalloproteins for light-induced electron transfer. Proc Natl Acad Sci U S A, 95(20), 11526-31, 1998 PMCID:21674

van den Brink, HB, et al.. Neutral-pion electroproduction on the proton near threshold. Phys. Rev. Lett., 74, 3561-3564, 1995

Bobeldijk, I, et al.. High-momentum protons in /sup 208/Pb. Phys. Rev. Lett., 73, 2684-2687, 1994


Postdoctoral Position Available
No

Postdoctoral Position Details
N/A

Updated Date
09/13/2013