Biomedical Research Education & Training
Faculty Member

M'Koma, Amosy E, M.D.,MS.,Ph.D
Assistant Professor of Biochemistry and Cancer Biology at Meharry Medical College
Adjunct Associate Professor of Surgery at Vanderbilt University School of Medicine

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Phone Number: 615-327-6796

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M'Koma, Amosy's picture
Academic history
M.D., Kharkov Medical Institute, Ukraine
Surgical Residency, Karolinska University Hospital, Huddinge, Sweden
Licentiate of Medical Science, Karolinska Nobel Institute, Sweden
Ph.D., Karolinska Nobel Institute, Sweden
Postdoctoral Fellow, Vanderbilt University School of Medicine, U.S.A

Office Address   Mailing Address

1005 Dr. D.B. Todd Jr. Boulevard, Nasville, TN 37208-3599

Vanderbilt University Department of Surgery, Colon & Rectal Surgery 615-343-4612

Research Keywords
Vanderbilt University, Vanderbilt-Ingram Cancer Center, Meharry Medical College and Tennessee State University (TSU) Cancer Partnership. Biopathophysiology of Inflammatory Bowel Disease (IBD). IBD associated Malignancy. Proteomics to improve Diagnostic Accuracy in IBD. Develop a Molecular Biometric Tool for Early Colon Cancer Detection for Accurate, Easy, Affordable and Noninvasive and Fast Screening. ,Biochemistry,Cancer,Endocrinology,Genome,Genomics,Immunology,Malignancy,Mass spectroscopy,Molecular medicine,Pathology,Physiology,Protein Structure,Proteomics,Recombination,Translation

Clinical Research Keywords
Our work focuses on the improvement of diagnostic accuracy using molecular biomarker profiling to discriminate inflammatory bowel diseases (IBD (UC, CC and IC)). The goal is to study differentially expressed proteomic patterns that occur as a result of the inflammatory colitides. We believe that the proteomic signatures between the colitides differs and will eventually enable more informed pharmacologic and surgical management by more definitively identifying their underlying diagnosis. We analyze tissue proteomes using a strategy based on matrix-assisted laser desorption-ionization mass spectroscopy (MALDI MS) and recombinant single-chain antibody to identify peptides found in tissue and later in serum to provide diagnostic and discriminatory markers for IBD. These researches are important for diagnostic accuracy as relates specifically to the understanding of the physiology of the restoration of intestinal continuity after operation for IBD. The research is a long-term and continued effort related to the physiology of restorative ileal pouches after proctocolectomy for IBD.

Research Specialty
One of the greatest advances in colorectal surgery over the past three decades has been the development of restorative proctocolectomy (RPC) with ileal pouch-anal anastomosis (IPAA) for patients suffering from ulcerative colitis (UC) and familial adenomatous polyposis (FAP). The development and refinement of pelvic pouch surgery now allows the entire excision of a diseased colon while maintaining transanal fecal continence. The success of RPC is largely dependent on careful patient selection combined with meticulous surgical technique. RPC is now the criterion surgical procedure, for patients with IBD. When this procedure first became the standard of care little was known about the metabolic consequences of the operation. We began several prospective studies investigating surgical complications, function of the pouches, consequences of pouch inflammation (pouchitis), new trends for ileal reservoir construction and health related quality of life (QoL) issues. We also developed several laboratory tests to detect possible metabolic effects of RPC (see publications). The patient's QoL and satisfaction are high and functional outcome is excellent, even after 20 years of functional pouches. The critical challenge we still face is the means to establish a correct diagnosis in IBD. An accurate diagnosis prior to initiating medical therapy or performing colectomy is of paramount importance in terms of evidence-based personalized medical therapy, surgical intervention and prognosis, as well as delineation of IBD by non-invasive, easier, accurate and faster screening.

Research Description
The research is focused on understanding the pathophysiology of IBD, particularly on diagnostic methodologies and surgical management of UC, specifically relating to the physiology of RPC or IPAA. The current research efforts are directed towards developing strategic methodologies based on matrix-assisted laser desorption-ionization-mass spectrometry (MALDI MS), proteomics and recombinant single-chain antibodies to identify peptides that can provide discriminatory diagnostic tools for inflammatory colitis.

Patients with IBD are susceptible to long-term complications from the disease and medications prescribed. The available cure may require surgical removal of the entire colon; however there are complications such as pouchitis/ cufittis, dysplasia, stricture and/ or fistulae. Why these complications affect patients with IBD after RPC differently has not been studied. There are no known preoperative predictor(s) of these complications. The ability to predict these outcomes can allow for prophylactic measures and prevention of pathologic outcomes. Thus our future studies would need to include further elucidation/ verification of the recombinant antibodies in larger patient study cohorts in order to understand the associated biomarker(s) that will be used in future endeavors to help:
-Clarify any proteomic differences of dysplasia within colitides.
-Predict future direction of indeterminate colitis (IC) into UC or CC.
-Predict CC/UC pts that are likely to develop colitis-associated colon or/and rectal cancer.
-Predict which patients with CC are likely to develop stricture or fistulae.
-Predict which patients with UC are likely to develop pouchitis or/ and cufittis following RPC.

Clinical Research Description
The treatment options for UC and CC differ significantly. Therefore the accurate diagnosis of IBD is of paramount importance in terms of personalized medical treatment, surgical intervention and prognosis. The distinction between UC and CC cannot be differentiated in 15% of IBD patients due to the fact that the pathological features of UC and CC often overlap. As a result, the CC patients undergo RPC surgery for definitive UC and subsequently are found to develop Crohn's diseases in the pouch. This is critical because the higher complication and functional failure rates (up to 60%) often necessitates excision of the pouch resulting in a permanent end ileostomy, a complication of which both patients and doctors would like to avoid. Additionally, failure to recognize characteristic signs of CC such as granulomas and transmural inflammation often leads to errors in pathological interpretation of IBD.
Another 15% cannot definitively be categorized as UC or CC. This condition is labeled as "indeterminate colitis" (IC). About 90% of patients diagnosed with IC undergo emergency surgery for fulminate colitis, contrasting with only 30% of patients in whom UC or CC are more confidently diagnose. We aim to try to identify molecular features that distinguish UC from CC. We hypothesize that, the proteomic pattern candidates that permit distinguishing UC and CC may allow predicting IC patients into UC or CC. The early accurate distinction of the pathology of IC could alleviate the discomfort in the significantly growing pediatric population.
Our goal is to initiating a successful research program in the area of colorectal surgery and establishes a feasibility of identifying a panel of novel molecular signatures in IBD. The research is also focused to further pursue the understanding of the biopathophysiologic mechanisms of the identified discriminatory molecules by examining the mucosal immune response in IBD.

Ballard BR, M'Koma AE. Gastrointestinal endoscopy biopsy derived proteomic patterns predict indeterminate colitis into ulcerative colitis and Crohn's colitis. World J Gastrointest Endos, 7(7), 670-674, 2015

Korolkova OY, Myers JN, Pellom ST, Wang L, M'Koma AE. Characterization of Serum Cytokine Profile in Predominantly Colonic Inflammatory Bowel Disease to Delineate Ulcerative and Crohn's Colitides.. Clin Med Insights Gastroenterol, 8(5), 29-44, 2015

Vaiopoulou A, Gazouli M, Papadopoulou A, Anagnostopoulos AK, Karamanolis G, Theodoropoulos GE, M'Koma A, Tsangaris GT.. Serum Protein Profiling of Adults And Children With Crohn's Disease. J Pediatr Gastroenterol Nutr, 60(1), 42-47, 2015

Williams AD, M'Koma AE. Molecular differentiation of ulcerative colitis and Crohn's colitis: is it achievable?. Clinical Laboratory International(November), 2015

M'Koma AE. Diagnosis of inflammatory bowel disease: Potential role of molecular biometrics. World J Gastrointest Surg, 6(11), 208-219, 2014

Myers JN, Schaffer MW, Korolkova OY, Williams AD, Gangula PR, M'Koma AE. Implications of the Colonic Deposition of Free Hemoglobin-a Chain: A Previously Unknown Tissue By-product in Inflammatory Bowel Disease. Inflamm Bowel Dis, 20(9), 1530-47, 2014

M'Koma AE. Letter to the Editor, Advances in Enzyme Research. Advances in Enzyme Research (AER) , 1(4), 77-78, 2013

M'Koma AE. Inflammatory Bowel Disease: An Expanding Global Health Problem. Clinical Medicine Insight: Gastroenterology(6), 33-47, 2013

M'Koma AE, Herline AJ, Adunyah SE. Subsequent Adenomas of Ileal Pouch and Anorectal Segment After Prophylactic Surgery for Familial Adenomatous Polyposis. World Journal of Colorectal Surgery, 3(2), Article 1, 2013

Seeley, EH, Washington, MK, Caprioli, RM, M''Koma, AE. Proteomic patterns of colonic mucosal tissues delineate Crohn''s colitis and ulcerative colitis. Proteomics Clin Appl, 7(7-8), 541-9, 2013

Smith JC, Schaeffer MW, Ballard BR, Smoot DT, Herline AJ, Adunyah SE, M'Koma AE . Adenocarcinomas after Prophylactic Surgery for Familial Adenomatous Polyposis. Journal of Cancer Ther, 4(1), 260-270, 2013

M'Koma AE, Moses HL, Adunyah SE. Inflammatory bowel disease-associated colorectal cancer: proctocolectomy and mucosectomy do not necessarily eliminate pouch-related cancer incidences. Int J Colorectal Dis, 26(5), 533-552, 2011

M'Koma AE, Seeley EH, Washington MK, Schwartz DA, Muldoon RL, Herline AJ, Wise PE, Caplioli RM.. Proteomic Profiling of Mucosal and Submucosal Colonic Tissues Yields Protein Signatures that Differentiate the Inflammatory Colitides. Inflamm Bowel Dis, 17(4), 875-883, 2011

Um JW, M'Koma AE. Dysplasie und Adenokarzinome im Ileum-Pouch nach restaurativer Proktokolektomie wegen Colitis ulcerosa. Coloproctology, 33(5), 273-282, 2011

Um JW, M'Koma AE. Pouch-related dysplasia and adenocarcinoma following restorative proctocolectomy for ulcerative colitis.. Tech Coloproctol, 15(1), 7-16, 2011

Amosy M'Koma. Biochemical Laboratory Data Following Restorative Proctocolectomy: Observations on Essential Biochemical Data Profile in Connection with Restorative Proctocolectomy in Humans . Book, ISBN 978-3-8383-2057-1(P Lambert Academic Publishing), 1-140, 2010

M''Koma, AE, Wise, PE, Schwartz, DA, Muldoon, RL, Herline, AJ. Prevalence and outcome of anemia after restorative proctocolectomy: a clinical literature review. Dis Colon Rectum, 52(4), 726-39, 2009

M'Koma AE, Wise PE, Schwartz DA, Muldoon RL, Herline AJ.. Prevalence and Outcome of Anemia after Restorative Proctocolectomy. Clinical Literature Review.. Dis Colon Rectum, 52(4), 726-736, 2009

Blum DL, Koyama T, M'Koma AE, Iturregui JM, Martinez-Ferrer M, Uwamariya C, Smith Jr JA, Clark PE, Bhowmick NE. Chemokine markers predict biochemical recurrence of prostate cancer following prostatectomy. Clin Cancer Res, 14(23), 7790-7, 2008

Blum, DL, Koyama, T, M''Koma, AE, Iturregui, JM, Martinez-Ferrer, M, Uwamariya, C, Smith, JA, Clark, PE, Bhowmick, NA. Chemokine markers predict biochemical recurrence of prostate cancer following prostatectomy. Clin Cancer Res, 14(23), 7790-7, 2008

M'Koma, AE, Blum, DL, Norris, JL, Koyama, T, Billheimer, D, Motley, S, Ghiassi, M, Ferdowsi, N, Bhowmick, I, Chang, SS, Fowke, JH, Caprioli, RM, Bhowmick, NA. Detection of pre-neoplastic and neoplastic prostate disease by MALDI profiling of urine. Biochem Biophys Res Commun, 353(3), 829-34, 2007 PMCID:2562600

M'Koma, AE, Longo, WE. Postoperative liver enzyme abnormalities are related to staged restorative proctocolectomy. Int J Colorectal Dis, 22(3), 283-8, 2007

M'Koma, AE, Wise, PE, Muldoon, RL, Schwartz, DA, Washington, MK, Herline, AJ. Evolution of the restorative proctocolectomy and its effects on gastrointestinal hormones. Int J Colorectal Dis, 22(10), 1143-63, 2007

M'Koma, AE. Serum biochemical evaluation of patients with functional pouches ten to 20 years after restorative proctocolectomy. Int J Colorectal Dis, 21(7), 711-20, 2006

Swedish Government. States Committee of inquiry into HIV/ AIDS. Swedish Ministry of Heath, SOU 2004:13(ISBN 91-38-220806), 2004

M'Koma AE. Sex and HIV in the city: economy of desire in urban Tanzania. HIV Perspektiv, 310(14), 846-849, 2003

MKoma AE. Access to Care: Privilage or Right? Migration and HIV Vulnerability in Europe. AIDS mobility Europe(October), 127-136, 2003

M'Koma AE. Myths, beliefs, taboos and sexual behaviour. The Second National conference on HIV/STD-prevention. Swedish Institute of Public Health/ Kunskap Utveckling, 1:25(October 18-19), 36, 2001

M'Koma AE. Observation on Biochemical Data Profile in Connection with Restorative Proctocolectomy in Humans. Vitamin B12 and Fat Absorption Cited. Karolinska University Press, 1-130, 2001

M'Koma AE. European Partenariat between Migrant communities and Health Partners for HIV/AIDS Prevention in Europe. Swedish Institute of Public Health/ Kunskap Utveckling, 1:26(October 18-19), 37, 2001

M'Koma AE, (Sweden); Revault P, Fanget D (France); Partidario A (Portugal); Boahene K (The Netherlands); Castillo S (Spain); Murray A (UK); Louhenapessy M (Belgium). European partenariat with African communities. XIII International AIDS2000 Congress, MoPeD2842, 2000

M'Koma AE, Samaletdin D, Hedman H. AIDS is future threat to African future . HIV perspective , 10(10), 567-574, 2000

M'Koma, AE, Lindquist, K, Liljeqvist, L. A study of plasma immunoglobulins profile in connection with restorative proctocolectomy. Int J Surg Investig, 2(2), 125-31, 2000

M'Koma, AE, Lindquist, K, Liljeqvist, L. Observations in the blood lipid profile in patients undergoing restorative proctocolectomy. Int J Surg Investig, 2(3), 227-35, 2000

MKoma AE, Revault P, Fanget D; Partidario A, Boahene K, Castillo S, Murray A, Louhenapessy M . Partenariat Europaen entre communautes africaines et acteurs de sante pour la prention du VIH/sida. Rapport deactivite intermediaire - S12.83594 (99CFV4-006)(Juin GRDR/Secretariat), 2000

M'Koma, AE, Lindquist, K, Liljeqvist, L. Effect of restorative proctocolectomy on gastric acid secretion and serum gastrin levels: a prospective study. Dis Colon Rectum, 42(3), 398-402, 1999

MKoma AE. Observation on Biochemical Data in Connection with Restorative Proctocolectomy (an analysis with specific reference to lipoproteins and gastric acids. Karolinska University Press, 1999

M'Koma AE. Demographic Data and HIV Risk in Tanzania. HIV perspective, 7(14), 391-392, 1998

M'Koma AE. Editor: Jeanette de Putter. AIDS & STDs and migrants, ethnic minorities and other mobile groups. The State of Affairs in Europe (AIDS Mobility), 2007-2008, 1998

MKoma AE, Lindquist K, Liljeqvist L. Effect of restorative proctocolectomy on gastric acid secretion and serum gastrin levels. A controlled prospective study. XVII Biennial Congress of the International Society of University Colon and Rectum Surgeons (ISUCRS), Malmo, Sweden(June 7-11), PC173, 1998

M'Koma AE, Lindquist K, Liljeqvist L. Effect of restorative proctocolectomy on gastric acid secretion and serum gastrin levels. A controlled prospective study. Eur Surg Res, XXXII Congress of the European Surgical Research (ESSR), Corfu, Greece, 29 suppl 1(May 18-21), 4, 1997

M'Koma AE, Lindquist K, Liljeqvist L. Effect of restorative proctocolectomy on lipid metabolism . Eur Surg Res, XXXII Congress of the European Surgical Research (ESSR), Corfu, Greece, 29 suppl 1(May 18-21), 3, 1997

MKoma AE, Makonnen G. Evaluation of home based cultural and professionally care and counselling to PLWHIV/AIDS. X International Conference on STD/AIDS in Africa, Abidjan Cote dIvoire, 10 ICASA(December 7-11), C-008, 1997

MKoma AE, Makonnen G. Nzamba J. AIDS Outreach program targeting Academics from Africa to promote behaviour change . X International Conference on STD/AIDS in Africa, Abidjan Cote dIvoire, 10 ICASA(December 7-11), E-579, 1997

MKoma AE, Lindgren S.. Migration and Health. Gender and HIV/AIDS awareness in the African population of Sweden. Umea University Hospital, Department of family medicine and Karolinska Institute, Huddinge University Hospital, Department of Clinical Science, Division of Obstetrics and Gynaecology, 1996

Eriksson L, Kosmowski AM, MKoma AE. Long jail sentence to drug trafficker women from Africa and the HIV/ AIDS. International Conference on AIDS and STD in Africa (ICASA), Kampala, Uganda, TuD603, 1995

M'Koma AE. Report from AIDS IMPACT Conference. Stockholm County Council AIDS Prevention Program Journal (LAFA) (1), 24, 1995

M'Koma AE. The HIV Psychosocial impact on the African community in Sweden. International Conference on AIDS and STD (ICASA), Kampala, Uganda, TuD672, 1995

M'Koma AE. Institutional unawareness of immigrants competence. Swedish Daily News(January 21), B16, 1995

M'Koma AE, Lindquist K, Liljeqvist L.. Adaptive returns to normal serum electrolytes after depletion following restorative proctocolectomy and ileal-pouch anal anstomosis. A controlled, prospective study. Int J Surg Sci(2), 330-334, 1995

M'Koma AE. Report from the VII International Conference on HIV/ STDs In Africa. Immigrants in Sweden against Narcotics Journal, S I M O N(1), 22-27, 1994

M'Koma AE. Migrants, Ethnic Minority and Substance Abuse: Experiences from Sweden. AIDS Mobility Europe(Leeuwenhortst, The Netherlands), Appex 4, 1994

M'Koma, AE. Follow-up results of hematology data before and after restorative proctocolectomy. Clinical outcome. Dis Colon Rectum, 37(9), 932-7, 1994

M'Koma, AE, Lindquist, K, Liljeqvist, L. Biochemical laboratory data in patients before and after restorative proctocolectomy. A study on 83 patients with a follow-up of 36 months. Ann Chir, 48(6), 525-34, 1994

M'Koma AE. Migration and Health a Global Problem, Etiology and possible solutions. Immigrants in Sweden against Narcotics Journal, S I M O N(1), 5-6, 1993

M'Koma AE. Screening Africans in the EU borders does not prevent HIV-spread. Solution is to increase information. Swedish Daily News(June, 6), A07, 1993

M'Koma AE, Makonnen G. Nzanba J . HIV-spread among Drug user immigrants in Stockholm - Sweden. 8th International Congress on AIDS and STD in Africa (ICASA) Marrakech, Morocco, 8th ICASA(December 12-16 ), 126, 1993

M'Koma AE, Lindquist K, Liljeqvist L . Chemical Laboratory Data in patients Undergoing Ileoanal Pouch Surgery. Annales de Chirurgie, Ileal pouch-anal anastomosis, International Symposium. Versailles, France (September 18-19), 15:26, 1992

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