Biomedical Research Education & Training
Faculty Member

Chekmenev, Eduard Y, PhD
Assistant Professor of Radiology and Radiological Sciences
Assistant Professor of Biomedical Engineering

Lab Url: http://www.chekmenev.com

Phone Number: (615) 322-1329

Email Address: eduard.chekmenev@vanderbilt.edu

Chekmenev, Eduard's picture
Academic history
B.S., Perm State University, Perm, Russia
M.S., University of Louisville, Louisville, KY
Ph.D., University of Louisville, Louisville, KY

Office Address   Mailing Address

AA-3117 VUIIS

1161 21st Avenue South Medical Center North, AA-1105 37232-2310


Research Keywords
Instrumentation, chemistry and spin physics of hyperpolarized metabolic MRI tracers in biomedical research. Real time metabolic imaging of breast cancer using hyperpolarized MRI tracers. ,Cancer,Malignancy,Mouse,NMR,Receptor

Research Description
The long term goal of our research is to answer the questions about cancer metabolic disorders at molecular, cellular and tissue level and its correlation with genetic disorders and pathology. We focus on development of the predictors (often termed biomarkers) about the patient outcome and response to therapy as early as several minutes after drug administration using real time metabolic imaging. Funded projects (by NIH/NCI and Prevent Cancer Foundation) in our laboratory are conducted in cellular and rodent models of human cancer utilizing Magnetic Resonance Imaging (MRI) and its variant Magnetic Resonance Spectroscopic Imaing (MRSI) modalities. We use MR hyperpolared 13C and 15N labeled metabolic contrast agents to achieve unprecedented spatial resolution and high contrast. The advantage of the hyperpolarization techniques is the increase in MR sensitivity by 10,000-1,000,000 fold, which overcomes previous sensitivity limitations of MRI. Our laboratory at VUIIS currently utilizes parahydrogen gas and commercially availabe hyperpolarized 129Xe gas to hyperpolarize 13C and 15N contrast agents. These contrast agents are non-radioactive and use no ionizing radiation during imaging and enable a new generation of ultrasensitive, ultrafast MR imaging techniques that will be optimized for use in oncology. The persistence of polarization through chemical reactions of biochemical pathways allows sub-second MRI and MRSI examinations in real time whereas current standard of care in oncology PET-CT exam requires long examiation time and expose patients to ionizing radiation. We also hope to address the central issues, necessary for successful introduction of Clinical Trials of non-invasive and non-radioactive hyperpolarized MRSI using injectacble hyperpolarized choline, glutamate, glutamine and succinate and others as in vivo contrast imaging reagents. These biomarkers potentially allow direct imaging of real time metabolic activity of choline kinase (ChoK), succinate dehydrogenase (SDH), etc. as well as indirect imaging of hypoxia inducing factor HIF-1I? and other oncogenes. We also work on receptor imaging using hyperpolarized MR, which can be useful for in vivo cancer research as well as for in vitro structural and functional studies of proteins and especially membrane associated proteins. We would like to demonstrate efficacy of hyperpolarized biomarkers for early detection of cancer and response to treatment using sub-second MRI and ultrafast MRSI and demonstrate the advantages of hyperpolarized metabolic tracers in defining tumor growth, heterogeneity and prediction of a positive response to therapy, when compared to conventional MRI and PET-CT.

Ultrafast hyperpolarized MRSI will have far-reaching impact on all areas of oncology in which current imaging technologies are insufficiently precise or insensitive to early diagnosis. We hope not only investigate underlying metabolic events of cancer with real-time metabolic imaging in laboratory setting, but also provide US population with fast, safe low-cost metabolic MR exams in the future that will be useful for population screening and treatment follow-up and would replace or augment ionizing mammography screening and expensive radioactive PET-CT.

Publications
Nikolaou, P, Coffey, AM, Walkup, LL, Gust, BM, Lapierre, CD, Koehnemann, E, Barlow, MJ, Rosen, MS, Goodson, BM, Chekmenev, EY. A 3D-Printed High Power Nuclear Spin Polarizer. J Am Chem Soc, 2014

Perrin, BS, Tian, Y, Fu, R, Grant, CV, Chekmenev, EY, Wieczorek, WE, Dao, AE, Hayden, RM, Burzynski, CM, Venable, RM, Sharma, M, Opella, SJ, Pastor, RW, Cotten, ML. High-Resolution Structures and Orientations of Antimicrobial Peptides Piscidin 1 and Piscidin 3 in Fluid Bilayers Reveal Tilting, Kinking, and Bilayer Immersion. J Am Chem Soc, 2014

Cai, C, Coffey, AM, Shchepin, RV, Chekmenev, EY, Waddell, KW. Efficient transformation of parahydrogen spin order into heteronuclear magnetization. J Phys Chem B, 117(5), 1219-24, 2013

Chekmenev, EY. MRI Hyperpolarization and Molecular Imaging. The Newsletter of the SNMMI, 7(3), 1-3, 2013

Coffey, AM, Truong, ML, Chekmenev, EY. Low-field MRI can be more sensitive than high-field MRI. J Magn Reson, 237, 169-74, 2013

Nikolaou, P, Coffey, AM, Walkup, LL, Gust, BM, Whiting, N, Newton, H, Barcus, S, Muradyan, I, Dabaghyan, M, Moroz, GD, Rosen, MS, Patz, S, Barlow, MJ, Chekmenev, EY, Goodson, BM. Near-unity nuclear polarization with an open-source 129Xe hyperpolarizer for NMR and MRI. Proc Natl Acad Sci U S A, 110(35), 14150-5, 2013

Zu, Z, Xu, J, Li, H, Chekmenev, EY, Quarles, CC, Does, MD, Gore, JC, Gochberg, DF. Imaging amide proton transfer and nuclear overhauser enhancement using chemical exchange rotation transfer (CERT). Magn Reson Med, 2013

Abramson, RG, Arlinghaus, LR, Weis, JA, Li, X, Dula, AN, Chekmenev, EY, Smith, SA, Miga, MI, Abramson, VG, Yankeelov, TE. Current and emerging quantitative magnetic resonance imaging methods for assessing and predicting the response of breast cancer to neoadjuvant therapy. Breast Cancer (London), 2012(4), 139-154, 2012

Coffey, AM, Shchepin, RV, Wilkens, K, Waddell, KW, Chekmenev, EY. A large volume double channel 1H-X RF probe for hyperpolarized magnetic resonance at 0.0475 T. J Magn Reson, 220, 94-101, 2012

Feng, B, Coffey, AM, Colon, RD, Chekmenev, EY, Waddell, KW. A pulsed injection parahydrogen generator and techniques for quantifying enrichment. J Magn Reson, 214(1), 258-62, 2012

Shchepin, RV, Coffey, AM, Waddell, KW, Chekmenev, EY. PASADENA hyperpolarized 13C phospholactate. J Am Chem Soc, 134(9), 3957-60, 2012

Shchepin, RV, Coffey, AM, Waddell, KW, Chekmenev, EY. Parahydrogen Induced Polarization with Rh-based Monodentate Ligand in Water. J Phys Chem Lett, 3(22), 3281-3285, 2012

Bhattacharya, P, Chekmenev, EY, Reynolds, WF, Wagner, S, Zacharias, N, Chan, HR, B??nger, R, Ross, BD. Parahydrogen-induced polarization (PHIP) hyperpolarized MR receptor imaging in vivo: a pilot study of 13C imaging of atheroma in mice. NMR Biomed, 24(8), 1023-8, 2011

Harrington, MG, Chekmenev, EY, Schepkin, V, Fonteh, AN, Arakaki, X. Sodium MRI in a rat migraine model and a NEURON simulation study support a role for sodium in migraine. Cephalalgia, 31(12), 1254-65, 2011

Kurhanewicz, J, Vigneron, DB, Brindle, K, Chekmenev, EY, Comment, A, Cunningham, CH, Deberardinis, RJ, Green, GG, Leach, MO, Rajan, SS, Rizi, RR, Ross, BD, Warren, WS, Malloy, CR. Analysis of cancer metabolism by imaging hyperpolarized nuclei: prospects for translation to clinical research. Neoplasia, 13(2), 81-97, 2011 PMCID:3033588

Waddell, KW, Coffey, AM, Chekmenev, EY. In situ detection of PHIP at 48 mT: demonstration using a centrally controlled polarizer. J Am Chem Soc, 133(1), 97-101, 2011

Chekmenev, EY, Vollmar, BS, Cotten, M. Can antimicrobial peptides scavenge around a cell in less than a second. Biochim Biophys Acta, 1798(2), 228-34, 2010

Chekmenev, EY, Norton, VA, Weitekamp, DP, Bhattacharya, P. Hyperpolarized (1)H NMR Employing Low gamma Nucleus for Spin Polarization Storage. J Am Chem Soc, 131(9), 3164-5, 2009 PMCID:2662390

Hoevener, JB, Chekmenev, EY, Harris, KC, Perman, WH, Robertson, LW, Ross, BD, Bhattacharya, P. PASADENA hyperpolarization of 13C biomolecules: equipment design and installation. MAGMA, 22(2), 111-21, 2009 PMCID:2664858

Hoevener, JB, Chekmenev, EY, Harris, KC, Perman, WH, Tran, TT, Ross, BD, Bhattacharya, P. Quality assurance of PASADENA hyperpolarization for 13C biomolecules. MAGMA, 22(2), 123-34, 2009 PMCID:2664864

Lisitza, N, Muradian, I, Frederick, E, Patz, S, Hatabu, H, Chekmenev, EY. Toward 13C hyperpolarized biomarkers produced by thermal mixing with hyperpolarized 129Xe. J Chem Phys, 131(4), 044508, 2009 PMCID:2730707

Chekmenev, EY, Bhattacharya, P, Ross, BD. Development of Hyperpolarized Metabolic Contrast Agents using PASADENA. Cambridge Isotope Laboratories, Application Note, #21, 2008

Chekmenev, EY, Chow, SK, Tofan, D, Weitekamp, DP, Ross, BD, Bhattacharya, P. Fluorine-19 NMR chemical shift probes molecular binding to lipid membranes. J Phys Chem B, 112(20), 6285-7, 2008 PMCID:2663341

Chekmenev, EY, Hoevener, J, Norton, VA, Harris, K, Batchelder, LS, Bhattacharya, P, Ross, BD, Weitekamp, DP. PASADENA hyperpolarization of succinic acid for MRI and NMR spectroscopy. J Am Chem Soc, 130(13), 4212-3, 2008 PMCID:2662769

Bhattacharya, P, Chekmenev, EY, Perman, WH, Harris, KC, Lin, AP, Norton, VA, Tan, CT, Ross, BD, Weitekamp, DP. Towards hyperpolarized (13)C-succinate imaging of brain cancer. J Magn Reson, 186(1), 150-5, 2007 PMCID:2657725

Gor''kov, PL, Chekmenev, EY, Li, C, Cotten, M, Buffy, JJ, Traaseth, NJ, Veglia, G, Brey, WW. Using low-E resonators to reduce RF heating in biological samples for static solid-state NMR up to 900 MHz. J Magn Reson, 185(1), 77-93, 2007

Gor''kov, PL, Witter, R, Chekmenev, EY, Nozirov, F, Fu, R, Brey, WW. Low-E probe for (19)F-(1)H NMR of dilute biological solids. J Magn Reson, 189(2), 182-9, 2007

Buffy, JJ, Traaseth, NJ, Mascioni, A, Gor''kov, PL, Chekmenev, EY, Brey, WW, Veglia, G. Two-dimensional solid-state NMR reveals two topologies of sarcolipin in oriented lipid bilayers. Biochemistry, 45(36), 10939-46, 2006

Chekmenev, EY, Gor''kov, PL, Cross, TA, Alaouie, AM, Smirnov, AI. Flow-through lipid nanotube arrays for structure-function studies of membrane proteins by solid-state NMR spectroscopy. Biophys J, 91(8), 3076-84, 2006 PMCID:1578476

Chekmenev, EY, Jones, SM, Nikolayeva, YN, Vollmar, BS, Wagner, TJ, Gor''kov, PL, Brey, WW, Manion, MN, Daugherty, KC, Cotten, M. High-field NMR studies of molecular recognition and structure-function relationships in antimicrobial piscidins at the water-lipid bilayer interface. J Am Chem Soc, 128(16), 5308-9, 2006

Chekmenev, EY, Vollmar, BS, Forseth, KT, Manion, MN, Jones, SM, Wagner, TJ, Endicott, RM, Kyriss, BP, Homem, LM, Pate, M, He, J, Raines, J, Gor''kov, PL, Brey, WW, Mitchell, DJ, Auman, AJ, Ellard-Ivey, MJ, Blazyk, J, Cotten, M. Investigating molecular recognition and biological function at interfaces using piscidins, antimicrobial peptides from fish. Biochim Biophys Acta, 1758(9), 1359-72, 2006

Chekmenev, EY, Waddell, KW, Hu, J, Gan, Z, Wittebort, RJ, Cross, TA. Ion-binding study by 17O solid-state NMR spectroscopy in the model peptide Gly-Gly-Gly at 19.6 T. J Am Chem Soc, 128(30), 9849-55, 2006

Gor''kov, PL, Chekmenev, EY, Fu, R, Hu, J, Cross, TA, Cotten, M, Brey, WW. A large volume flat coil probe for oriented membrane proteins. J Magn Reson, 181(1), 9-20, 2006

Hu, J, Chekmenev, EY, Cross, TA. Anisotropic Chemical Shift Perturbation Induced by Ions in Conducting Channels. Modern Magnetic Resonance, 3, 279-283, 2006

Li, C, Mo, Y, Hu, J, Chekmenev, E, Tian, C, Gao, FP, Fu, R, Gor''kov, P, Brey, W, Cross, TA. Analysis of RF heating and sample stability in aligned static solid-state NMR spectroscopy. J Magn Reson, 180(1), 51-7, 2006

Waddell, KW, Chekmenev, EY, Wittebort, RJ. Peptide 17O chemical shielding and electric field gradient tensors. J Phys Chem B, 110(45), 22935-41, 2006

Chekmenev, EY, Hu, J, Gor''kov, PL, Brey, WW, Cross, TA, Ruuge, A, Smirnov, AI. 15N and 31P solid-state NMR study of transmembrane domain alignment of M2 protein of influenza A virus in hydrated cylindrical lipid bilayers confined to anodic aluminum oxide nanopores. J Magn Reson, 173(2), 322-7, 2005

Fu, R, Brey, WW, Shetty, K, Gor''kov, P, Saha, S, Long, JR, Grant, SC, Chekmenev, EY, Hu, J, Gan, Z, Sharma, M, Zhang, F, Logan, TM, Br??schweller, R, Edison, A, Blue, A, Dixon, IR, Markiewicz, WD, Cross, TA. Ultra-wide bore 900 MHz high-resolution NMR at the National High Magnetic Field Laboratory. J Magn Reson, 177(1), 1-8, 2005

Hu, J, Chekmenev, EY, Gan, Z, Gor''kov, PL, Saha, S, Brey, WW, Cross, TA. Ion solvation by channel carbonyls characterized by 17O solid-state NMR at 21 T. J Am Chem Soc, 127(34), 11922-3, 2005

Waddell, KW, Chekmenev, EY, Wittebort, RJ. Single-crystal studies of peptide prolyl and glycyl 15N shielding tensors. J Am Chem Soc, 127(25), 9030-5, 2005

Chekmenev, EY, Zhang, Q, Waddell, KW, Mashuta, MS, Wittebort, RJ. 15N Chemical shielding in glycyl tripeptides: measurement by solid-state NMR and correlation with X-ray structure. J Am Chem Soc, 126(1), 379-84, 2004

Fan, T. W. M.; Lane, A. N.; Chekmenev, E.; Wittebort, R. J.; Higashi, R. M.. Synthesis and physico-chemical properties of peptides in soil humic substances. J. Peptide Res., 63(3), 253-264, 2004

Pometun, MS, Chekmenev, EY, Wittebort, RJ. Quantitative observation of backbone disorder in native elastin. J Biol Chem, 279(9), 7982-7, 2004

Zhang, Q, Chekmenev, EY, Wittebort, RJ. 17O quadrupole coupling and chemical shielding tensors in an H-bonded carboxyl group: alpha-oxalic acid. J Am Chem Soc, 125(30), 9140-6, 2003

Chekmenev, EY, Xu, RZ, Mashuta, MS, Wittebort, RJ. Glycyl C(alpha) chemical shielding in tripeptides: measurement by solid-state NMR and correlation with X-ray structure and theory. J Am Chem Soc, 124(40), 11894-9, 2002


Postdoctoral Position Available
No

Postdoctoral Position Details
N/A

Updated Date
02/02/2014