Biomedical Research Education & Training
Faculty Member

Means, Anna L., Ph.D.
Research Assistant Professor of Surgery
Assistant Professor of Cell and Developmental Biology

Lab Url: http://www.vanderbilthealth.com/diabetes/25923

Phone Number: 343-0922

Email Address: anna.means@vanderbilt.edu

Means, Anna's picture
Academic history
B.S., Ohio Univ
Ph.D., Univ of Wisconsin

Office Address   Mailing Address

D2300 Medical Center North

D2300 Medical Center North Dept. of Surgery 37232-2733


Research Keywords
Pancreas, development, cancer, epithelial-mesenchymal interaction, cell signaling, epidermal growth factor receptor, transdifferentiation, islet neogenesis, tumor progression, tumor microenvironment,Cancer,Developmental biology,Diabetes,Gene regulation,Kinase,Knockout,Malignancy,Mouse,Mutation,Receptor,Signal transduction

Research Specialty
Pancreatic cancer; tumor microenvironment; growth factor signaling; pancreas development; oncogenes

Research Description
Work in my lab investigates signaling pathways that regulate cell-cell interactions during development of pancreatic cancer. In particular, we are interested in how the epidermal growth factor receptor (EGFR) and one of its ligands, heparin-binding epidermal growth factor-like growth factor (HB-EGF), coordinately regulate both epithelial tumorigenesis and the fibrosis that forms the microenvironment surrounding the developing tumor. We have found that the initiation of pancreatic cancer does not follow the classic paradigm seen in colon cancer where loss of a tumor suppressor gene initiates tumorigenesis and then acquisition of an oncogene promotes tumor formation. The pancreas seems to require two oncogenic events to initiate and promote tumorigenesis with tumor suppressors acting later to increase progression to malignant disease. In the mouse models we have designed, the two oncogenes that are sufficient to initiate and promote pancreatic cancer are KrasG12D and elevated expression of HB-EGF. We are currently understanding how these two oncogenes synergize at the molecular level. We are also investigating targeting the normal source of HB-EGF, inflammatory cells, to determine whether HB-EGF alone or in concert with other cytokines produced by inflammatory cells is necessary for tumor formation.

In a related line of investigation, we have found that overexpression of HB-EGF also increases fibrosis in the pancreas. Fibrosis is a consistent character in pancreatic cancer and likely provides the signals necessary for survival and growth of the tumor and well as providing a barrier to chemotherapeutic treatment. We are using mouse models in conjunction with human tissue analysis to understand how this fibrosis arises and how it can be altered to allow access of chemotherapeutic agents to the tumor.

We have found that epithelium and mesenchyme/stroma are also coordinately regulated during embryonic development. Understanding how interactions between these tissues are regulated will indicate pathways that may also function in those interactions that occur during tumorigenesis in the adult. During embryonic development, mesenchyme (embryonic stroma) is critical for pancreatic development even though only the epithelium gives rise to the pancreas. Removal of surrounding mesenchyme prevents growth and alters the differentiation profile of the pancreatic epithelium. We have found that blocking EGFR signaling has a similar effect to removing the surrounding mesenchyme -- growth is severely compromised and differentiation is altered. We have localized EGFR protein at the epithelial-mesenchymal interface, supporting our hypothesis that EGFR signaling mediates the crosstalk between these two tissues.

All of this work uses a variety of experimental approaches. We use transgenes and gene knockouts to study regulatory processes in vivo. Whenever possible, we correlate our findings back to human pancreatic cancer tissues. We also do culture of cells and tissues isolated from mice to manipulate signaling events in controlled, measurable ways. We use physiology, histology, and molecular biology to understand development and disease on a holistic basis, all the way from the individual to the organ to the tissue and finally to the molecules within cells.


Publications
Ray, KC, Bell, KM, Yan, J, Gu, G, Chung, CH, Washington, MK, Means, AL. Epithelial tissues have varying degrees of susceptibility to Kras(G12D)-initiated tumorigenesis in a mouse model. PLoS One, 6(2), e16786, 2011 PMCID:3032792

Blaine, SA, Ray, KC, Anunobi, R, Gannon, MA, Washington, MK, Means, AL. Adult pancreatic acinar cells give rise to ducts but not endocrine cells in response to growth factor signaling. Development, 137(14), 2289-96, 2010

Blaine, SA, Ray, KC, Branch, KM, Robinson, PS, Whitehead, RH, Means, AL. Epidermal growth factor receptor regulates pancreatic fibrosis. Am J Physiol Gastrointest Liver Physiol, 297(3), G434-41, 2009 PMCID:2739824

Ray, KC, Blaine, SA, Washington, MK, Braun, AH, Singh, AB, Harris, RC, Harding, PA, Coffey, RJ, Means, AL. Transmembrane and soluble isoforms of heparin-binding epidermal growth factor-like growth factor regulate distinct processes in the pancreas. Gastroenterology, 137(5), 1785-94, 2009 PMCID:2767440

Zhang, H, Ables, ET, Pope, CF, Washington, MK, Hipkens, S, Means, AL, Path, G, Seufert, J, Costa, RH, Leiter, AB, Magnuson, MA, Gannon, M. Multiple, temporal-specific roles for HNF6 in pancreatic endocrine and ductal differentiation. Mech Dev, 126(11-12), 958-73, 2009

Means, AL, Xu, Y, Zhao, A, Ray, KC, Gu, G. A CK19(CreERT) knockin mouse line allows for conditional DNA recombination in epithelial cells in multiple endodermal organs. Genesis, 46(6), 318-23, 2008

Means, AL, Chytil, A, Moses, HL, Coffey, RJ, Wright, CV, Taketo, MM, Grady, WM. Keratin 19 gene drives Cre recombinase expression throughout the early postimplantation mouse embryo. Genesis, 42(1), 23-7, 2005

Means, AL, Meszoely, IM, Suzuki, K, Miyamoto, Y, Rustgi, AK, Coffey, RJ, Wright, CV, Stoffers, DA, Leach, SD. Pancreatic epithelial plasticity mediated by acinar cell transdifferentiation and generation of nestin-positive intermediates. Development, 132(16), 3767-76, 2005

Nomura, S, Settle, SH, Leys, CM, Means, AL, Peek, RM, Leach, SD, Wright, CV, Coffey, RJ, Goldenring, JR. Evidence for repatterning of the gastric fundic epithelium associated with M??n??trier's disease and TGFalpha overexpression. Gastroenterology, 128(5), 1292-305, 2005

Matsuoka, Taka-aki, Artner, Isabella, Henderson, Eva, Means, Anna, Sander, Maike, Stein, Roland. The MafA transcription factor appears to be responsible for tissue-specific expression of insulin. Proc Natl Acad Sci U S A, 101(9), 2930-3, 2004 PMCID:365722

Matsuoka, Taka-aki, Zhao, Li, Artner, Isabella, Jarrett, Harry W, Friedman, David, Means, Anna, Stein, Roland. Members of the large Maf transcription family regulate insulin gene transcription in islet beta cells. Mol Cell Biol, 23(17), 6049-62, 2003 PMCID:180917

Means, Anna L, Ray, Kevin C, Singh, Amar B, Washington, M Kay, Whitehead, Robert H, Harris, Raymond C, Wright, Christopher V E, Coffey, Robert J, Leach, Steven D. Overexpression of heparin-binding EGF-like growth factor in mouse pancreas results in fibrosis and epithelial metaplasia. Gastroenterology, 124(4), 1020-36, 2003

Samaras, Susan E, Zhao, Li, Means, Anna, Henderson, Eva, Matsuoka, Taka-Aki, Stein, Roland. The islet beta cell-enriched RIPE3b1/Maf transcription factor regulates pdx-1 expression. J Biol Chem, 278(14), 12263-70, 2003

Means, A L, Leach, S D. Lineage commitment and cellular differentiation in exocrine pancreas. Pancreatology, 1(6), 587-96, 2001

Meszoely, I M, Means, A L, Scoggins, C R, Leach, S D. Developmental aspects of early pancreatic cancer. Cancer J, 7(4), 242-50, 2001

Means, A L, Thompson, J R, Gudas, L J. Transcriptional regulation of the cellular retinoic acid binding protein I gene in F9 teratocarcinoma cells. Cell Growth Differ, 11(2), 71-82, 2000

Scoggins, C R, Meszoely, I M, Wada, M, Means, A L, Yang, L, Leach, S D. p53-dependent acinar cell apoptosis triggers epithelial proliferation in duct-ligated murine pancreas. Am J Physiol Gastrointest Liver Physiol, 279(4), G827-36, 2000

Means, A L, Gudas, L J. The CRABP I gene contains two separable, redundant regulatory regions active in neural tissues in transgenic mouse embryos. Dev Dyn, 209(1), 59-69, 1997

Means, A L, Gudas, L J. FGF-2, BMP-2, and BMP-4 regulate retinoid binding proteins and receptors in 3T3 cells. Cell Growth Differ, 7(8), 989-96, 1996

Means, A L, Gudas, L J. The roles of retinoids in vertebrate development. Annu Rev Biochem, 64, 201-33, 1995

Means, A L, Slansky, J E, McMahon, S L, Knuth, M W, Farnham, P J. The HIP1 binding site is required for growth regulation of the dihydrofolate reductase gene promoter. Mol Cell Biol, 12(3), 1054-63, 1992 PMCID:369537

Farnham, P J, Means, A L. Sequences downstream of the transcription initiation site modulate the activity of the murine dihydrofolate reductase promoter. Mol Cell Biol, 10(4), 1390-8, 1990 PMCID:362241

Means, A L, Farnham, P J. Transcription initiation from the dihydrofolate reductase promoter is positioned by HIP1 binding at the initiation site. Mol Cell Biol, 10(2), 653-61, 1990 PMCID:360863


Postdoctoral Position Available
No

Postdoctoral Position Details
N/A

Updated Date
06/04/2012