Biomedical Research Education & Training
Faculty Member

Liebler, Daniel C., Ph.D.
Professor of Biochemistry
Professor of Biomedical Informatics
Professor of Pharmacology

Lab Url: https://my.vanderbilt.edu/liebler/

Phone Number: (615) 322-3063

Email Address: daniel.liebler@vanderbilt.edu

Liebler, Daniel's picture
Academic history
B.S., Villanova University, Villanova, PA
Ph.D., Vanderbilt University, Nashville, TN
Post-doc, Oregon State University, Corvallis, OR

Office Address   Mailing Address

U1213 Medical Research Building III

U1213 MRBIII 37232-6350


Research Keywords
protein modifications, biomarkers, mass spectrometry,Biochemistry,Cancer,Mass spectroscopy,Proteomics,Toxicology

Research Specialty
Proteomic biomarkers for cancer detection and diagnosis; analysis and mechanisms of cell injury by reactive electrophiles

Research Description
Dr. Liebler?s research program addresses two broad problems that are linked by the application of analytical proteomics technologies, particularly mass spectrometry. The first problem is the development of protein biomarkers as diagnostics for early cancer detection and for guiding therapy of established disease. Application of standardized mass spectrometry-based proteomics technology platforms enables discovery and analytical validation of candidate biomarkers in human clinical specimens. Current work is directed at development of plasma-based diagnostics for early detection of colon and lung cancer and tumor tissue-based diagnostics for identifying high-risk cancers and predicting response to therapy.

The second problem is the role protein damage by reactive electrophiles in chemical toxicity and disease processes. This work involves development of methods to analyze modified and damaged proteins and to assess the impact of protein damage by reactive chemicals. Major areas of current focus are the characterization of global protein modification by endogenous lipid electrophiles in oxidative stress and the identification of signaling sensors targeted by electrophiles and the mechanisms by which chemical modification of these sensor proteins initiate stress responses.


Publications
Codreanu, SG, Kim, HY, Porter, NA, Liebler, DC. Biotinylated probes for the analysis of protein modification by electrophiles. Methods Mol Biol, 803, 77-95, 2012 PMCID:3271737

Halvey, PJ, Zhang, B, Coffey, RJ, Liebler, DC, Slebos, RJ. Proteomic consequences of a single gene mutation in a colorectal cancer model. J Proteome Res, 11(2), 1184-95, 2012 PMCID:3271737

Le, A, Lane, AN, Hamaker, M, Bose, S, Gouw, A, Barbi, J, Tsukamoto, T, Rojas, CJ, Slusher, BS, Zhang, H, Zimmerman, LJ, Liebler, DC, Slebos, RJ, Lorkiewicz, PK, Higashi, RM, Fan, TW, Dang, CV. Glucose-independent glutamine metabolism via TCA cycling for proliferation and survival in B cells. Cell Metab, 15(1), 110-21, 2012

Myers, MV, Manning, HC, Coffey, RJ, Liebler, DC. Protein Expression Signatures for Inhibition of Epidermal Growth Factor Receptor-mediated Signaling. Mol Cell Proteomics, 11(2), M111.015222, 2012 PMCID:3277773

Wang, X, Slebos, RJ, Wang, D, Halvey, PJ, Tabb, DL, Liebler, DC, Zhang, B. Protein Identification Using Customized Protein Sequence Databases Derived from RNA-Seq Data. J Proteome Res, 11(2), 1009-17, 2012 PMCID:3277773

Zimmerman, LJ, Li, M, Yarbrough, WG, Slebos, R, Liebler, DC. Global stability of plasma proteomes for mass spectrometry-based analyses. Mol Cell Proteomics, 2012

Becker, LC, Bergfeld, WF, Belsito, DV, Hill, RA, Klaassen, CD, Liebler, DC, Marks, JG, Shank, RC, Slaga, TJ, Snyder, PW, Andersen, FA. Final report of the Cosmetic Ingredient Review Expert Panel safety assessment of polymethyl methacrylate (PMMA), methyl methacrylate crosspolymer, and methyl methacrylate/glycol dimethacrylate crosspolymer. Int J Toxicol, 30(3 Suppl), 54S-65S, 2011 PMCID:3208394

Bose, D, Zimmerman, LJ, Pierobon, M, Petricoin, E, Tozzi, F, Parikh, A, Fan, F, Dallas, N, Xia, L, Gaur, P, Samuel, S, Liebler, DC, Ellis, LM. Chemoresistant colorectal cancer cells and cancer stem cells mediate growth and survival of bystander cells. Br J Cancer, 105(11), 1759-67, 2011 PMCID:3242606

Burnett, CL, Bergfeld, WF, Belsito, DV, Hill, RA, Klaassen, CD, Liebler, DC, Marks, JG, Shank, RC, Slaga, TJ, Snyder, PW, Andersen, FA. Final report of the Amended Safety Assessment of PVM/MA copolymer and its related salts and esters as used in cosmetics. Int J Toxicol, 30(5 Suppl), 128S-44S, 2011 PMCID:3277773

Connor, RE, Marnett, LJ, Liebler, DC. Protein-selective capture to analyze electrophile adduction of hsp90 by 4-hydroxynonenal. Chem Res Toxicol, 24(8), 1275-82, 2011 PMCID:3155980

Hoofnagle, AN, Aebersold, R, Anderson, NL, Felsenfeld, A, Liebler, DC. Painting a moving picture: large-scale proteomics efforts and their potential for changing patient care. Clin Chem, 57(10), 1357-60, 2011 PMCID:3155980

Johnson, W, Bergfeld, WF, Belsito, DV, Hill, RA, Klaassen, CD, Liebler, DC, Marks, JG, Shank, RC, Slaga, TJ, Snyder, PW, Andersen, FA. Amended safety assessment of Sesamum indicum (sesame) seed oil, hydrogenated sesame seed oil, Sesamum indicum (sesame) oil unsaponifiables, and sodium sesameseedate. Int J Toxicol, 30(3 Suppl), 40S-53S, 2011 PMCID:3208394

Johnson, W, Bergfeld, WF, Belsito, DV, Hill, RA, Klaassen, CD, Liebler, DC, Marks, JG, Shank, RC, Slaga, TJ, Snyder, PW, Andersen, FA. Safety assessment of cyclomethicone, cyclotetrasiloxane, cyclopentasiloxane, cyclohexasiloxane, and cycloheptasiloxane. Int J Toxicol, 30(6 Suppl), 149S-227S, 2011

Li, J, Su, Z, Ma, ZQ, Slebos, RJ, Halvey, P, Tabb, DL, Liebler, DC, Pao, W, Zhang, B. A bioinformatics workflow for variant peptide detection in shotgun proteomics. Mol Cell Proteomics, 10(5), M110.006536, 2011 PMCID:3098595

Ma, ZQ, Tabb, DL, Burden, J, Chambers, MC, Cox, MB, Cantrell, MJ, Ham, AJ, Litton, MD, Oreto, MR, Schultz, WC, Sobecki, SM, Tsui, TY, Wernke, GR, Liebler, DC. Supporting tool suite for production proteomics. Bioinformatics, 27(22), 3214-5, 2011 PMCID:3208394

Michaelson-Richie, ED, Ming, X, Codreanu, SG, Loeber, RL, Liebler, DC, Campbell, C, Tretyakova, NY. Mechlorethamine-induced DNA-protein cross-linking in human fibrosarcoma (HT1080) cells. J Proteome Res, 10(6), 2785-96, 2011 PMCID:3208907

Rexer, BN, Ham, AJ, Rinehart, C, Hill, S, Granja-Ingram, Nde M, Gonz?!lez-Angulo, AM, Mills, GB, Dave, B, Chang, JC, Liebler, DC, Arteaga, CL. Phosphoproteomic mass spectrometry profiling links Src family kinases to escape from HER2 tyrosine kinase inhibition. Oncogene, 30(40), 4163-74, 2011 PMCID:3204390

Schepeler, T, Holm, A, Halvey, P, Nordentoft, I, Lamy, P, Riising, EM, Christensen, LL, Thorsen, K, Liebler, DC, Helin, K, Orntoft, TF, Andersen, CL. Attenuation of the beta-catenin/TCF4 complex in colorectal cancer cells induces several growth-suppressive microRNAs that target cancer promoting genes. Oncogene, 2011 PMCID:3208394

Zhang, B, Shi, Z, Duncan, DT, Prodduturi, N, Marnett, LJ, Liebler, DC. Relating protein adduction to gene expression changes: a systems approach. Mol Biosyst, 7(7), 2118-27, 2011 PMCID:3155980

Zhang, H, Liu, Q, Zimmerman, LJ, Ham, AJ, Slebos, RJ, Rahman, J, Kikuchi, T, Massion, PP, Carbone, DP, Billheimer, D, Liebler, DC. Methods for peptide and protein quantitation by liquid chromatography-multiple reaction monitoring mass spectrometry. Mol Cell Proteomics, 10(6), M110.006593, 2011 PMCID:3108838


Postdoctoral Position Available
Yes

Postdoctoral Position Details
Please send letter of application, CV and names and contact information for three references to daniel.liebler@vanderbilt.edu.

Updated Date
02/24/2012