Biomedical Research Education & Training
Faculty Member

Bader, David M., Ph.D.
Professor of Medicine
Gladys Parkinson Stahlman Chair in Cardiovascular Research
Professor of Cell and Developmental Biology

Lab Url: N/A

Phone Number: 615-936-1976

Email Address:

Bader, David's picture
Academic history
B.A., Augustana College, Sioux Falls, S.D.
Ph.D., University of North Dakota, Grand Forks, N.D.

Office Address   Mailing Address

346 Preston Research Building

346 PRB 2220 Pierce AVenue 37232-6300

Research Keywords
Bves, Lek1, development, heart, cell adhesion, cell migration, cytoskeleton, organogensis

Research Description
The work in our laboratory has focused on the commitment, differentiation and function of cardiac and vascular cells in the embryo. The early heart development and vasculogenesis provide excellent systems to examine fundamental issues in developmental biology. Heart development involves the commitment of embryogenic cells to the cardiogenic mesodermal lineage, coordinated activation of cell-specific genes in committed cells, and the diversification of progenitors in a highly patterned manner leading to the generation of distinct myogenic phenotypes.

Our current work is focused on the function of two genes that were discovered in our laboratory and analysis of the conserved system whereby coelomates generate vessels to organs during development.

LEK1 is a large and complex protein that regulates proliferation and differentiation of cardiac myocytes.
Through a series of biochemical, morphological, genetic and cell biological experiments, we have determined that LEK1 proteins function in cell movement, trafficking and division through its interaction with Rb proteins, the cytoskeleton and the SNARE complex. Our ongoing work is focused on conditionally inhibiting the function of this gene in developing mice and continuing our studies of LEK1 interaction with subcellular domains.

Bves is an integral membrane protein discovered by former members of the lab. This protein is essential for proper cell/cell interaction during coronary vessel development. Our work has shown that Bves is one of the first proteins trafficked to points of cell/cell contact and that it associates with cytoplasmic proteins that regulate cell/cell adhesion, process formation and movement. Our goal is to determine how disruption of Bves function impacts embryonic development in vertebrate and invertebrate development.

Lastly, we have determined that blood vessel formation to internal organs has a conserved program that is been overlooked heretofore. We have discovered that vasculogenesis to the heart and gut is linked to the formation of the embryonic coelom and that progenitors of these blood vessels arise via a mechanism unlike that of the body wall and limbs. We are now disrupting this developmental system in an effort to understand the cellular and molecular mechanisms driving this process.

Pfaltzgraff, ER, Shelton, EL, Galindo, CL, Nelms, BL, Hooper, CW, Poole, SD, Labosky, PA, Bader, DM, Reese, J. Embryonic domains of the aorta derived from diverse origins exhibit distinct properties that converge into a common phenotype in the adult. J Mol Cell Cardiol, 2014

Benesh, EC, Miller, PM, Pfaltzgraff, ER, Grega-Larson, NE, Hager, HA, Sung, BH, Qu, X, Baldwin, HS, Weaver, AM, Bader, DM. Bves and NDRG4 regulate directional epicardial cell migration through autocrine extracellular matrix deposition. Mol Biol Cell, 24(22), 3496-510, 2013

Shelton, EL, Galindo, CL, Williams, CH, Pfaltzgraff, E, Hong, CC, Bader, DM. Autotaxin signaling governs phenotypic heterogeneity in visceral and parietal mesothelia. PLoS One, 8(7), e69712, 2013

Dees, E, Miller, PM, Moynihan, KL, Pooley, RD, Hunt, RP, Galindo, CL, Rottman, JN, Bader, DM. Cardiac-specific deletion of the microtubule-binding protein CENP-F causes dilated cardiomyopathy. Dis Model Mech, 5(4), 468-80, 2012

Shelton, EL, Bader, DM. Thymosin ??4 mobilizes mesothelial cells for blood vessel repair. Ann N Y Acad Sci, 1269, 125-30, 2012

Shelton, EL, Poole, SD, Reese, J, Bader, DM. Omental grafting: a cell-based therapy for blood vessel repair. J Tissue Eng Regen Med, 2012

Thomason, RT, Bader, DM, Winters, NI. Comprehensive timeline of mesodermal development in the quail small intestine. Dev Dyn, 241(11), 1678-94, 2012

Winters, NI, Thomason, RT, Bader, DM. Identification of a novel developmental mechanism in the generation of mesothelia. Development, 139(16), 2926-34, 2012

Cross, EE, Thomason, RT, Martinez, M, Hopkins, CR, Hong, CC, Bader, DM. Application of Small Organic Molecules Reveals Cooperative TGFI? and BMP Regulation of Mesothelial Cell Behaviors. ACS Chem Biol, 2011

Russ, PK, Pino, CJ, Williams, CS, Bader, DM, Haselton, FR, Chang, MS. Bves modulates tight junction associated signaling. PLoS One, 6(1), e14563, 2011 PMCID:3024319

Hager, HA, Roberts, RJ, Cross, EE, Proux-Gillardeaux, V, Bader, DM. Identification of a novel Bves function: regulation of vesicular transport. EMBO J, 29(3), 532-45, 2010

Karunamuni, G, Yang, K, Doughman, YQ, Wikenheiser, J, Bader, D, Barnett, J, Austin, A, Parsons-Wingerter, P, Watanabe, M. Expression of lymphatic markers during avian and mouse cardiogenesis. Anat Rec (Hoboken), 293(2), 259-70, 2010

Hager, HA, Bader, DM. Bves: ten years after. Histol Histopathol, 24(6), 777-87, 2009

Moynihan, KL, Pooley, R, Miller, PM, Kaverina, I, Bader, DM. Murine CENP-F regulates centrosomal microtubule nucleation and interacts with Hook2 at the centrosome. Mol Biol Cell, 20(22), 4790-803, 2009 PMCID:2777108

Yang, K, Doughman, YQ, Karunamuni, G, Gu, S, Yang, YC, Bader, DM, Watanabe, M. Expression of active Notch1 in avian coronary development. Dev Dyn, 238(1), 162-70, 2009 PMCID:2777108

Kawaguchi, M, Hager, HA, Wada, A, Koyama, T, Chang, MS, Bader, DM. Identification of a novel intracellular interaction domain essential for Bves function. PLoS ONE, 3(5), e2261, 2008 PMCID:2373926

Pooley, RD, Moynihan, KL, Soukoulis, V, Reddy, S, Francis, R, Lo, C, Ma, LJ, Bader, DM. Murine CENPF interacts with syntaxin 4 in the regulation of vesicular transport. J Cell Sci, 121(Pt 20), 3413-21, 2008 PMCID:2777108

Robertson, J.B., Zhu, T., Nasreen, S., Kilenny, D., Bader, D., and Dees, E.. CMF1-Rb interaction promotes myogenesis in avain skeletal myoblasts. Dev Dyn, 237, 1424-1433, 2008

Robertson, JB, Zhu, T, Nasreen, S, Kilkenny, D, Bader, D, Dees, E. CMF1-Rb interaction promotes myogenesis in avian skeletal myoblasts. Dev Dyn, 237(5), 1424-33, 2008 PMCID:2373926

Smith, TK, Hager, HA (co-first author), Francis, R, Kilkenny, DM, Lo, CW, Bader, DM. Bves directly interacts with GEFT, and controls cell shape and movement through regulation of Rac1/Cdc42 activity. Proc Natl Acad Sci U S A, 105(24), 8298-303, 2008 PMCID:2423412

Kawaguchi, M, Bader, DM, Wilm, B. Serosal mesothelium retains vasculogenic potential. Dev Dyn, 236(11), 2973-9, 2007

Smith, TK, Bader, DM. Signals from both sides: Control of cardiac development by the endocardium and epicardium. Semin Cell Dev Biol, 18(1), 84-9, 2007

Brissova, M, Shostak, A, Shiota, M, Wiebe, PO, Poffenberger, G, Kantz, J, Chen, Z, Carr, C, Jerome, WG, Chen, J, Baldwin, HS, Nicholson, W, Bader, DM, Jetton, T, Gannon, M, Powers, AC. Pancreatic islet production of vascular endothelial growth factor--a is essential for islet vascularization, revascularization, and function. Diabetes, 55(11), 2974-85, 2006 PMCID:2373926

Dees, E, Robertson, JB, Zhu, T, Bader, D. Specific deletion of CMF1 nuclear localization domain causes incomplete cell cycle withdrawal and impaired differentiation in avian skeletal myoblasts. Exp Cell Res, 312(16), 3000-14, 2006 PMCID:2373926

Dees, E., Robertson, J.B., Zhu, T., and Bader, D.. Specific deletion of CMF1 nuclear localization domain causes incomplete cell cycle withdrawal and impaired differentiation in avian skeletal myoblasts.. Exp Cell Res, 312, 3000-3014, 2006

Osler, ME, Smith, TK, Bader, DM. Bves, a member of the Popeye domain-containing gene family. Dev Dyn, 235(3), 586-93, 2006

Pooley, RD, Reddy, S, Soukoulis, V, Roland, JT, Goldenring, JR, Bader, DM. CytLEK1 is a regulator of plasma membrane recycling through its interaction with SNAP-25. Mol Biol Cell, 17(7), 3176-86, 2006 PMCID:1483049

Ripley, AN, Osler, ME, Wright, CV, Bader, D. Xbves is a regulator of epithelial movement during early Xenopus laevis development. Proc Natl Acad Sci U S A, 103(3), 614-9, 2006 PMCID:1334639

Smith, TK, Bader, DM. Characterization of Bves expression during mouse development using newly generated immunoreagents. Dev Dyn, 235(6), 1701-8, 2006

Dees, E, Robertson, JB, Ashe, M, Pab??n-Pe??a, LM, Bader, D, Goodwin, RL. LEK1 protein expression in normal and dysregulated cardiomyocyte mitosis. Anat Rec A Discov Mol Cell Evol Biol, 286(1), 823-32, 2005

Osler, ME, Chang, MS, Bader, DM. Bves modulates epithelial integrity through an interaction at the tight junction. J Cell Sci, 118(Pt 20), 4667-78, 2005 PMCID:1483049

Soukoulis, V, Reddy, S, Pooley, RD, Feng, Y, Walsh, CA, Bader, DM. Cytoplasmic LEK1 is a regulator of microtubule function through its interaction with the LIS1 pathway. Proc Natl Acad Sci U S A, 102(24), 8549-54, 2005 PMCID:1150833

Wilm, B, Ipenberg, A, Hastie, ND, Burch, JB, Bader, DM. The serosal mesothelium is a major source of smooth muscle cells of the gut vasculature. Development, 132(23), 5317-28, 2005 PMCID:1483049

Osler, Megan E, Bader, David M. Bves expression during avian embryogenesis. Dev Dyn, 229(3), 658-67, 2004

Ripley, Anna N, Chang, Min S, Bader, David M. Bves is expressed in the epithelial components of the retina, lens, and cornea. Invest Ophthalmol Vis Sci, 45(8), 2475-83, 2004

Knight, RF, Bader, DM, Backstrom, JR. Membrane topology of Bves/Pop1A, a cell adhesion molecule that displays dynamic changes in cellular distribution during development. J Biol Chem, 278(35), 32872-9, 2003 PMCID:1150833

Wada, AM, Willet, SG, Bader, D. Coronary vessel development: a unique form of vasculogenesis. Arterioscler Thromb Vasc Biol, 23(12), 2138-45, 2003

Wada, Aya M, Smith, Travis K, Osler, Megan E, Reese, David E, Bader, David M. Epicardial/Mesothelial cell line retains vasculogenic potential of embryonic epicardium. Circ Res, 92(5), 525-31, 2003

Nesset, Anna L, Bader, David M. Hole is a novel gene product expressed in the developing heart and brain. Mech Dev, 117(1-2), 347-50, 2002

Wada, AM, Reese, DE, Bader, DM. Bves: prototype of a new class of cell adhesion molecules expressed during coronary artery development. Development, 128(11), 2085-93, 2001 PMCID:2373926

Croissant, JD, Carpenter, S, Bader, D. Identification and genomic cloning of CMHC1. A unique myosin heavy chain expressed exclusively in the developing chicken heart. J Biol Chem, 275(3), 1944-51, 2000

Dees, E, Pab??n-Pe??a, LM, Goodwin, RL, Bader, D. Characterization of CMF1 in avian skeletal muscle. Dev Dyn, 219(2), 169-81, 2000

Pab??n-Pe??a, LM, Goodwin, RL, Cise, LJ, Bader, D. Analysis of CMF1 reveals a bone morphogenetic protein-independent component of the cardiomyogenic pathway. J Biol Chem, 275(28), 21453-9, 2000

Goodwin, RL, Pab??n-Pe??a, LM, Foster, GC, Bader, D. The cloning and analysis of LEK1 identifies variations in the LEK/centromere protein F/mitosin gene family. J Biol Chem, 274(26), 18597-604, 1999

Reese, D E, Bader, D M. Cloning and expression of hbves, a novel and highly conserved mRNA expressed in the developing and adult heart and skeletal muscle in the human. Mamm Genome, 10(9), 913-5, 1999

Reese, DE, Zavaljevski, M, Streiff, NL, Bader, D. bves: A novel gene expressed during coronary blood vessel development. Dev Biol, 209(1), 159-71, 1999

Gannon, M, Bader, D. Avian cardiac progenitors: methods for isolation, culture, and analysis of differentiation. Methods Cell Biol, 52, 117-32, 1997

Eisenberg, C. and Bader, D. Establishment of a mesodermal cell line QCE-6. Circulation Research, 78, 205-216, 1996

Wei, Y., Bader, D., and Litvin, J. Identification of a cardiac-specific transcript essential for myocyte differentiation. Development, 122, 2779-2789, 1996

Eisenberg, C. and Bader, D. QCE-6: A clonal cell line with cardiac and endothelial cell potentials. Dev. Biol., 167, 469-481, 1995

Gannon, M. and Bader, D. Initiation of cardiac differentiation occurs in the absence of anterior endoderm. Development , 121, 2439-2450, 1995

Melnik, N. And Bader, D. Commitment, Differentiation, & Diversification of Avian Cardiac progenitor Cells. Annuals New York Academy of Sciences, 27(752), 1-8, 1995

Yutzey, K. and Bader, D. Diversification of cardiomyogenic cell lineages during early heart development . Circ. Res., 77, 216-219, 1995

Yutzey, K.E., Gannon. M., and Bader, D. Diversification of cardiomyogenic cell lineages in vitro . Developmental Biology, 170, 531-541, 1995

Montgomery, M.O., Litvin, J., Gonzalez-Sanchez, A., and Bader, D. In vitro analysis of the cardiomyogenic cell lineage. Dev. Biol., 164, 63-70, 1994

Yutzey, K.E., Rhee, J.T,, and Bader, D. Expression of the atrial-specific myosin heavy chain AMHC1 and the establishment of anteroposterior polarity in the developing chicken heart. Development , 120, 871-883, 1994

Litvin, J., Montgomery, M.O., Goldhamer, D., Emerson, C.P., and Bader, D. Identification of DNA-binding protein(s) in the developing heart. Devel. Biol., 156, 409-417, 1993

Han, Y.S., Dennis, J.E., Cohen-Gould, L., Bader, D., and Fischman, D.A. The expression and distribution of sarcomeric myosin in the presumptive myocardium of early chicken embryos. Dev. Dyn., 193, 257-265, 1992

Bisaha, J.G. and Bader, D. Isolation and characterization of a ventricular-specific avian myosin heavy chain, VMHC1: Expression in differentiating cardiac and skeletal muscle. Devel. Biol., 148, 355-364, 1991

Gonzalez-Sanchez, A. and D. Bader. In vitro analysis of cardiac progenitor cell differentiation. Devel. Biol., 139, 197-209, 1990

Zadeh, B.J., A. Gonzalez-Sanchez, D.A. Fischman, and D. Bader. Myosin heavy chain expression in embryonic cardiac cell cultures. Dev. Biol., 115, 204-214, 1986

Zhang, Y., S.A.Shafiq, and D. Bader. Detection of ventricular myosin heavy chain throughout cardiac myogenesis in the chicken heart. J. Cell Biol., 102, 1480-1484, 1986

Gonzalez-Sanchez, A. and D. Bader. Characterization of myosin heavy chain in myocardial cells of the conductive system of the chicken heart. J. Cell Biol., 100, 270-275, 1985

Gonzalez-Sanchez, A. and D. Bader. Immunochemical analysis of myosin heavy chains in the developing chicken heart. Develop. Biol., 103, 151-158, 1984

Bader, D.M., T. Masaki and D.A. Fischman. Immunochemical analysis of myosin heavy chains during avian myogenesis in vivo and in vitro. J. Cell Biol., 95(3), 763-770, 1982

Bader, D. and J.O. Oberpriller. Autoradiographic and electron microscopic studies of minced cardiac muscle regeneration in the adult newt (Notophthalmus viridescens). J. Exp. Zool., 208, 177-193, 1979

Bader, D. and J.O. Oberpriller. Repair and reorganization of cardiac muscle grafts in the adult newt (Notophthalmus viridescens). J. Morph., 155, 349-358, 1978

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