Biomedical Research Education & Training
Faculty Member

Reese, John Jeffrey, M.D.
Associate Professor of Pediatrics
Associate Professor of Cell and Developmental Biology

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Phone Number: 322-8643

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Reese, John's picture
Academic history
B.A., University of Kansas
M.D., University of Kansas
Pediatrics Residency, Vanderbilt University
Neonatal Registrar, Monash Medical Centre
Neonatology Fellowship, Yale University

Office Address   Mailing Address

1135 Light Hall/MRB IV bldg

1125 MRB IV 37232-0656

Research Description
Our lab is interested in the mechanisms of embryo-uterine interactions at the time of implantation and fetal-maternal interactions at the time of birth. During early pregnancy, a tightly regulated series of molecular signals coordinates uterine receptivity and activation of the developing embryo. In collaboration with other Vanderbilt investigators, we are examining the role of growth factors, cytokines and prostaglandins in the establishment of pregnancy. Genomic screens have also identified numerous factors that are upregulated at the time of implantation, but whose function in this process is unknown. Current research projects are aimed at resolving the contribution of these gene families to embryo implantation.

We are also examining the role of prostaglandins in term and preterm birth. Prostaglandins are products of arachidonic acid metabolism with vasoactive and mitogenic properties, and are likely downstream mediators of growth factor signaling. Cyclooxygenase (COX) is the rate-limiting enzyme in prostaglandin synthesis. COX-1 is the constitutive isoform of this enzyme whose expression is developmentally regulated, while COX-2 is expressed in response to inflammatory stimuli. We observed reduced vascular permeability and prostaglandin concentrations in the uteri of COX-1 deficient mice. However, COX-1 deficient uteri have compensatory upregulation of COX-2, suggesting a mechanism to preserve fertility. COX-1 null mice are also remarkable for their inability to complete labor or deliver healthy pups. We found that COX-1 deficient mice can deliver in a timely fashion when carrying a litter of wildtype pups (by embryo transfer). There are few mouse models that display parturition failure and offer the opportunity to examine the molecular basis of labor.

As part of these studies, COX-1/COX-2 double knockout mice have also been bred. These mice have perinatal lethality, possibly due to abnormal regulation of the ductus arteriosus, a fetal vascular shunt that is necessary for survival in-utero. Our newest area of research is to further evaluate the mechanisms of ductus arteriosus regulation using the mouse as a model. We are currently using mice with targeted deletions in both COX1 and COX2 and mice lacking a prostaglandin E receptor, which also die with a patent ductus arteriosus soon after birth. Studies in these mice may reveal novel mechanisms for the rapid sequence of vascular changes that take place during the transition from fetal to neonatal life. Together, these models are invaluable resources for the evaluation of prostaglandin actions in maternal-fetal communication during reproduction and during fetal organogenesis.


Clyman, RI, Wickremasinghe, A, Merritt, TA, Solomon, T, McNamara, P, Jain, A, Singh, J, Chu, A, Noori, S, Sekar, K, Lavoie, PM, Attridge, JT, Swanson, JR, Gillam-Krakauer, M, Reese, J, Demauro, S, Poindexter, B, Aucott, S, Satpute, M, Fernandez, E, Auchus, RJ, , . Hypotension following Patent Ductus Arteriosus Ligation: The Role of Adrenal Hormones. J Pediatr, 2014

El-Khuffash, A, Jain, A, Corcoran, D, Shah, PS, Hooper, CW, Brown, N, Poole, SD, Shelton, EL, Milne, GL, Reese, J, McNamara, PJ. Efficacy of Paracetamol on Patent Ductus Arteriosus Closure may be Dose Dependent: Evidence from human and murine studies. Pediatr Res, 2014

Huang, TK, Reese, J, Weitkamp, JH, Stancombe, BB. Congenital Radiocontrast Agent in the Neonatal Gut. J Pediatr, 2014

Lei, W, Herington, J, Galindo, CL, Ding, T, Brown, N, Reese, J, Paria, BC. Cross-species transcriptomic approach reveals genes in hamster implantation sites. Reproduction, 148(6), 607-21, 2014

McKnight, K, Hoang, HD, Prasain, JK, Brown, N, Vibbert, J, Hollister, KA, Moore, R, Ragains, JR, Reese, J, Miller, MA. Neurosensory perception of environmental cues modulates sperm motility critical for fertilization. Science, 344(6185), 754-7, 2014

Noori, S, McNamara, P, Jain, A, Lavoie, PM, Wickremasinghe, A, Merritt, TA, Solomon, T, Sekar, K, Attridge, JT, Swanson, JR, Gillam-Krakauer, M, Reese, J, Poindexter, BB, Brook, M, Auchus, RJ, Clyman, RI, , , , . Catecholamine-resistant hypotension and myocardial performance following patent ductus arteriosus ligation. J Perinatol, 2014

O''Brien, CM, Vargis, E, Paria, BC, Bennett, KA, Mahadevan-Jansen, A, Reese, J. Raman spectroscopy provides a non-invasive approach for determining biochemical composition of the pregnant cervix in vivo. Acta Paediatr, 2014

Pfaltzgraff, ER, Shelton, EL, Galindo, CL, Nelms, BL, Hooper, CW, Poole, SD, Labosky, PA, Bader, DM, Reese, J. Embryonic domains of the aorta derived from diverse origins exhibit distinct properties that converge into a common phenotype in the adult. J Mol Cell Cardiol, 2014

Shelton, EL, Ector, G, Galindo, CL, Hooper, C, Brown, N, Wilkerson, I, Pfaltzgraff, ER, Paria, BC, Cotton, RB, Stoller, JZ, Reese, J. Transcriptional profiling reveals ductus arteriosus-specific genes that regulate vascular tone. Physiol Genomics, 2014

Timmons, BC, Reese, J, Socrate, S, Ehinger, N, Paria, BC, Milne, GL, Akins, ML, Auchus, RJ, McIntire, D, House, M, Mahendroo, M. Prostaglandins are essential for cervical ripening in LPS-mediated preterm birth but not term or antiprogestin-driven preterm ripening. Endocrinology, 155(1), 287-98, 2014

Vucovich, MM, Cotton, RB, Shelton, EL, Goettel, JA, Ehinger, NJ, Poole, SD, Brown, N, Wynn, JL, Paria, BC, Slaughter, JC, Clark, RH, Rojas, MA, Reese, J. Aminoglycoside-mediated relaxation of the ductus arteriosus in sepsis-associated PDA. Am J Physiol Heart Circ Physiol, 307(5), H732-40, 2014

Clyman, R, Wickremasinghe, A, Jhaveri, N, Hassinger, DC, Attridge, JT, Sanocka, U, Polin, R, Gillam-Krakauer, M, Reese, J, Mammel, M, Couser, R, Mulrooney, N, Yanowitz, TD, Derrick, M, Jegatheesan, P, Walsh, M, Fujii, A, Porta, N, Carey, WA, Swanson, JR, , . Enteral feeding during indomethacin and ibuprofen treatment of a patent ductus arteriosus. J Pediatr, 163(2), 406-11, 2013

Cotton, RB, Shah, LP, Poole, SD, Ehinger, NJ, Brown, N, Shelton, EL, Slaughter, JC, Baldwin, HS, Paria, BC, Reese, J. Cimetidine-associated patent ductus arteriosus is mediated via a cytochrome P450 mechanism independent of H2 receptor antagonism. J Mol Cell Cardiol, 59, 86-94, 2013

Hermanson, DJ, Hartley, ND, Gamble-George, J, Brown, N, Shonesy, BC, Kingsley, PJ, Colbran, RJ, Reese, J, Marnett, LJ, Patel, S. Substrate-selective COX-2 inhibition decreases anxiety via endocannabinoid activation. Nat Neurosci, 16(9), 1291-8, 2013

Lei, W, Nguyen, H, Brown, N, Ni, H, Kiffer-Moreira, T, Reese, J, Millan, JL, Paria, B. Alkaline phosphatases contribute to uterine receptivity, implantation, decidualization and defense against bacterial endotoxin in hamsters. Reproduction, 2013

Ryckman, KK, Dagle, JM, Shchelochkov, OA, Ehinger, N, Poole, SD, Berberich, SL, Reese, J, Murray, JC. Association of amino acids with common complications of prematurity. Pediatr Res, 73(6), 700-5, 2013

Yanowitz, TD, Reese, J, Gillam-Krakauer, M, Cochran, CM, Jegatheesan, P, Lau, J, Tran, VT, Walsh, M, Carey, WA, Fujii, A, Fabio, A, Clyman, R. Superior Mesenteric Artery Blood Flow Velocities following Medical Treatment of a Patent Ductus Arteriosus. J Pediatr, 2013

Chen, JX, O''Mara, PW, Poole, SD, Brown, N, Ehinger, NJ, Slaughter, JC, Paria, BC, Aschner, JL, Reese, J. Isoprostanes as physiological mediators of transition to newborn life: novel mechanisms regulating patency of the term and preterm ductus arteriosus. Pediatr Res, 72(2), 122-8, 2012

Cotton, R, Suarez, S, Reese, J. Unexpected Extra-renal Effects of Loop Diuretics in the Preterm Neonate. Acta Paediatr, 2012

Johnston, PG, Gillam-Krakauer, M, Fuller, MP, Reese, J. Evidence-based use of indomethacin and ibuprofen in the neonatal intensive care unit. Clin Perinatol, 39(1), 111-36, 2012

Reese, J. Patent ductus arteriosus: mechanisms and management. Semin Perinatol, 36(2), 89-91, 2012

Shelton, EL, Poole, SD, Reese, J, Bader, DM. Omental grafting: a cell-based therapy for blood vessel repair. J Tissue Eng Regen Med, 2012

Stoller, JZ, Demauro, SB, Dagle, JM, Reese, J. Current Perspectives on Pathobiology of the Ductus Arteriosus. J Clin Exp Cardiolog, 8(1), 2012

Vargis, E, Brown, N, Williams, K, Al-Hendy, A, Paria, BC, Reese, J, Mahadevan-Jansen, A. Detecting Biochemical Changes in the Rodent Cervix During Pregnancy Using Raman Spectroscopy. Ann Biomed Eng, 2012

Vucovich, M, Ehinger, N, Poole, SD, Lamb, FS, Reese, J. Spontaneous Rhythmic Contractions (Vasomotion) of the Isolated, Pressurized Ductus Arteriosus of Preterm, but Not Term, Fetal Mice. EJ Neonatol Res, 2(1), 13-24, 2012

Luan, L, Ding, T, Stinnett, A, Reese, J, Paria, BC. Adherens Junction Proteins in the Hamster Uterus: Their Contributions to the Success of Implantation. Biol Reprod, 2011

McElroy, SJ, Prince, LS, Weitkamp, JH, Reese, J, Slaughter, JC, Polk, DB. Tumor necrosis factor receptor 1-dependent depletion of mucus in immature small intestine: a potential role in neonatal necrotizing enterocolitis. Am J Physiol Gastrointest Liver Physiol, 301(4), G656-66, 2011

Peterson, JA, Pechauer, SM, Gitter, ML, Kanack, A, Curtis, BR, Reese, J, Kamath, VM, McFarland, JG, Aster, RH. New platelet glycoprotein polymorphisms causing maternal immunization and neonatal alloimmune thrombocytopenia. Transfusion, 2011

Uddin, MJ, Crews, BC, Ghebreselasie, K, Huda, I, Kingsley, PJ, Ansari, MS, Tantawy, MN, Reese, J, Marnett, LJ. Fluorinated COX-2 inhibitors as agents in PET imaging of inflammation and cancer. Cancer Prev Res (Phila), 4(10), 1536-45, 2011

Wang, H, Luan, L, Ding, T, Brown, N, Reese, J, Paria, BC. Dynamics of zonula occludens-2 expression during preimplantation embryonic development in the hamster. Theriogenology, 76(4), 678-86, 2011

Reese, J, Veldman, A, Shah, L, Vucovich, M, Cotton, RB. Inadvertent relaxation of the ductus arteriosus by pharmacologic agents that are commonly used in the neonatal period. Semin Perinatol, 34(3), 222-30, 2010

Brown, N, Morrow, JD, Slaughter, JC, Paria, BC, Reese, J. Restoration of on-time embryo implantation corrects the timing of parturition in cytosolic phospholipase A2 group IVA deficient mice. Biol Reprod, 81(6), 1131-8, 2009

Reese, J, OMara, PW, Poole, SD, Brown, N, Tolentino, C, Eckman, DM, Aschner, JL. Regulation of the fetal mouse ductus arteriosus is dependent on interaction of nitric oxide and COX enzymes in the ductal wall. Prostaglandins Other Lipid Mediat, Apr;88(3-4), 89-96, 2009

Reese, J, Waleh, N, Poole, SD, Brown, N, Roman, C, Clyman, RI. Chronic in utero cyclooxygenase inhibition alters PGE2-regulated ductus arteriosus contractile pathways and prevents postnatal closure. Pediatr Res, Aug;66(2):, 155-61, 2009

Waleh, N, Reese, J, Kajino, H, Roman, C, Seidner, S, McCurnin, D, Clyman, RI. Oxygen-induced tension in the sheep ductus arteriosus: effects of gestation on potassium and calcium channel regulation. Pediatr Res, Mar;65(3):, 285-90, 2009 PMCID:2692833

Frank, DB, Lowery, J, Anderson, L, Brink, M, Reese, J, de Caestecker, M. Increased susceptibility to hypoxic pulmonary hypertension in Bmpr2 mutant mice is associated with endothelial dysfunction in the pulmonary vasculature. Am J Physiol Lung Cell Mol Physiol, 294(1), L98-109, 2008

Pyle, AL, Atkinson, JB, Pozzi, A, Reese, J, Eckes, B, Davidson, JM, Crimmins, DL, Young, PP. Regulation of the atheroma-enriched protein, SPRR3, in vascular smooth muscle cells through cyclic strain is dependent on integrin alpha1beta1/collagen interaction. Am J Pathol, 173(5), 1577-88, 2008 PMCID:2570146

Reese, J, Wang, H, Ding, T, Paria, BC. The hamster as a model for embryo implantation: insights into a multifaceted process. Semin Cell Dev Biol, 19(2), 194-203, 2008 PMCID:2288742

Wang, H, Ding, T, Brown, N, Yamamoto, Y, Prince, LS, Reese, J, Paria, BC. Zonula occludens-1 (ZO-1) is involved in morula to blastocyst transformation in the mouse. Dev Biol, 318(1), 112-25, 2008 PMCID:2442465

Tan, X, Essengue, S, Talreja, J, Reese, J, Stechschulte, DJ, Dileepan, KN. Histamine directly and synergistically with lipopolysaccharide stimulates cyclooxygenase-2 expression and prostaglandin I(2) and E(2) production in human coronary artery endothelial cells. J Immunol, 179(11), 7899-906, 2007 PMCID:2597490

Anderson, J, Brown, N, Mahendroo, MS, Reese, J. Utilization of different aquaporin water channels in the mouse cervix during pregnancy and parturition and in models of preterm and delayed cervical ripening. Endocrinology, 147(1), 130-40, 2006

Babaev, VR, Ding, L, Reese, J, Morrow, JD, Breyer, MD, Dey, SK, Fazio, S, Linton, MF. Cyclooxygenase-1 deficiency in bone marrow cells increases early atherosclerosis in apolipoprotein E- and low-density lipoprotein receptor-null mice. Circulation, 113(1), 108-17, 2006

Deb, K, Reese, J, Paria, BC. Methodologies to study implantation in mice. Methods Mol Med, 121, 9-34, 2006

Khatua, A, Wang, X, Ding, T, Zhang, Q, Reese, J, DeMayo, FJ, Paria, BC. Indian hedgehog, but not histidine decarboxylase or amphiregulin, is a progesterone-regulated uterine gene in hamsters. Endocrinology, 147(9), 4079-92, 2006

Reese, J. Death, dying, and exhaustion in the ductus arteriosus: prerequisites for permanent closure. Am J Physiol Regul Integr Comp Physiol, 290(2), R357-8, 2006

Reese, J, Anderson, JD, Brown, N, Roman, C, Clyman, RI. Inhibition of cyclooxygenase isoforms in late- but not midgestation decreases contractility of the ductus arteriosus and prevents postnatal closure in mice. Am J Physiol Regul Integr Comp Physiol, 291(6), R1717-1723, 2006

Brown, Naoko, Deb, Kaushik, Paria, Bibhash C., Das, Sanjoy K., Reese, Jeff. Embryo-Uterine Interactions via the Neuregulin Family of Growth Factors During Implantation in the Mouse. Biol Reprod, 2004

Dey, S K, Lim, H, Das, Sanjoy K, Reese, Jeff, Paria, B C, Daikoku, Takiko, Wang, Haibin. Molecular cues to implantation. Endocr Rev, 25(3), 341-73, 2004

Richard, Charissa, Gao, Ju, LaFleur, Bonnie, Christman, Brian W, Anderson, Judy, Brown, Naoko, Reese, Jeff. Patency of the preterm fetal ductus arteriosus is regulated by endothelial nitric oxide synthase and is independent of vasa vasorum in the mouse. Am J Physiol Regul Integr Comp Physiol, 287(3), R652-60, 2004

Wang, Xiaohong, Su, Yan, Deb, Kaushik, Raposo, Monika, Morrow, Jason D, Reese, Jeff, Paria, Bibhash C. Prostaglandin E2 is a product of induced prostaglandin-endoperoxide synthase 2 and microsomal-type prostaglandin E synthase at the implantation site of the hamster. J Biol Chem, 279(29), 30579-87, 2004

Gao, Ling, Zackert, William E, Hasford, Justin J, Danekis, Michael E, Milne, Ginger L, Remmert, Catha, Reese, Jeff, Yin, Huiyong, Tai, Hsin-Hsiung, Dey, Sudhansu K, Porter, Ned A, Morrow, Jason D. Formation of prostaglandins E2 and D2 via the isoprostane pathway: a mechanism for the generation of bioactive prostoglandins independent of cyclooxygenase. J Biol Chem, 278(31), 28479-89, 2003

Richard, Charissa, Gao, Ju, Brown, Naoko, Reese, Jeff. Aquaporin water channel genes are differentially expressed and regulated by ovarian steroids during the periimplantation period in the mouse. Endocrinology, 144(4), 1533-41, 2003

Lim, Hyunjung, Song, Haengseok, Paria, B C, Reese, Jeff, Das, Sanjoy K, Dey, S K. Molecules in blastocyst implantation: uterine and embryonic perspectives. Vitam Horm, 64, 43-76, 2002

Paria, B C, Reese, Jeff, Das, Sanjoy K, Dey, S K. Deciphering the cross-talk of implantation: advances and challenges. Science, 296(5576), 2185-8, 2002

Reese, J, Das, S K, Paria, B C, Lim, H, Song, H, Matsumoto, H, Knudtson, K L, DuBois, R N, Dey, S K. Global gene expression analysis to identify molecular markers of uterine receptivity and embryo implantation. J Biol Chem, 276(47), 44137-45, 2001

Reese, J, Zhao, X, Ma, W G, Brown, N, Maziasz, T J, Dey, S K. Comparative analysis of pharmacologic and/or genetic disruption of cyclooxygenase-1 and cyclooxygenase-2 function in female reproduction in mice. Endocrinology, 142(7), 3198-206, 2001

Paria, B C, Lim, H, Das, S K, Reese, J, Dey, S K. Molecular signaling in uterine receptivity for implantation. Semin Cell Dev Biol, 11(2), 67-76, 2000

Reese, J, Binart, N, Brown, N, Ma, W G, Paria, B C, Das, S K, Kelly, P A, Dey, S K. Implantation and decidualization defects in prolactin receptor (PRLR)-deficient mice are mediated by ovarian but not uterine PRLR. Endocrinology, 141(5), 1872-81, 2000

Reese, J, Paria, B C, Brown, N, Zhao, X, Morrow, J D, Dey, S K. Coordinated regulation of fetal and maternal prostaglandins directs successful birth and postnatal adaptation in the mouse. Proc Natl Acad Sci U S A, 97(17), 9759-64, 2000 PMCID:16938

Williams, C S, Tsujii, M, Reese, J, Dey, S K, DuBois, R N. Host cyclooxygenase-2 modulates carcinoma growth. J Clin Invest, 105(11), 1589-94, 2000 PMCID:300858

Reese, J, Brown, N, Paria, B C, Morrow, J, Dey, S K. COX-2 compensation in the uterus of COX-1 deficient mice during the pre-implantation period. Mol Cell Endocrinol, 150(1-2), 23-31, 1999

Reese, J, Brown, N, Das, S K, Dey, S K. Expression of neu differentiation factor during the periimplantation period in the mouse uterus. Biol Reprod, 58(3), 719-27, 1998

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