Biomedical Research Education & Training
Faculty Member

Limbird, Lee E., Ph.D.
Adjunct Professor of Medical Education and Administration

Lab Url: http://medschool.mc.vanderbilt.edu/limbirdlab/

Phone Number: (615) 322-2209

Email Address: lee.limbird@vanderbilt.edu

Limbird, Lee's picture
Academic history
B.A., College of Wooster, Wooster, Ohio
Ph.D., University of North Carolina, Chapel Hill, NC

Office Address   Mailing Address

464 Robinson Research Bldg.

417B PRB 6600


Research Keywords
Alpha2-AR Trafficking: Alpha2-Adrenergic Receptors | Structure-Function | G protein-coupled Receptors | Trafficking | 14-3-3 | Spinophilin | 3i loop | V2-Vasopressin Receptors

Function of Alpha2-AR in Vivo:


Research Specialty
G protein-coupled Receptors: Trafficking, signaling and in vivo function of alpha(2)-adrenergic receptor subtypes

Research Description
Our laboratory studies the structure, cellular and in vivo function, and trafficking of G protein-coupled receptors. The principal model system that we have used for our studies are the alpha(2)-adrenergic receptors (alpha(2)-ARs), which bind epinephrine and norepinephrine and elicit their diverse physiological effects by activation of Gi/Go GTP-binding proteins that regulate various electrical and chemical messengers. Investigation of genetically-modified mice with mutated, or absent, alpha(2)-ARs has revealed the role for the alpha(2A)-AR subtype in multiple physiological functions, including lowering of the blood pressure via the central nervous system, suppression of epileptogenesis, suppression of pain perception, sedation and anesthesic sparing. Continuing studies seek to understand the role of this subtype in a variety of behavioral paradigms, particularly those which link the receptor to roles in depression, attention, and cognition. Furthermore, with the advent of gene profiling and proteomics strategies, we have begun to explore genes and proteins expressed in critical brain regions in wildtype mice versus those lacking the gene expressing the alpha(2A)-AR subtype.

In a parallel line of studies in the laboratory, we are examining the mechanisms by which G protein-coupled receptors achieve their delivery and retention in specialized domains on the surface of target cells. For the alpha(2)-AR subtypes, we have focused primarily on polarized renal epithelial cells, where all three subtypes achieve basolateral localization at steady state, albeit via different trafficking itineraries. Structure-function studies have revealed that receptor sequences embedded in or near the bilayer are involved in receptor delivery to a particular surface, whereas the third intracellular (3i) loop of these three alpha(2)-AR subtypes is involved in tethering to the lateral subdomain. Recently, we have demonstrated that this 3i loop interacts both with 14-3-3 proteins as well as with spinophilin. Current studies are exploring the surface interface between the 3i loops and these interacting proteins as well as determining the functional relevance of these interactions for scaffolding, signaling or termination of signaling. These studies are being carried out both in vitro as well as in vivo, again using genetically-modified mice under development.

To reveal the molecules and machinery involved in G protein-coupled receptor targeting to a particular surface in renal epithelial cells, we have begun to study the V(2) vasopressin receptor system as a model. A variety of mutations within these receptors occur naturally, and lead to the disease X-linked nephrogenic diabetes insipidus. Most of these mutations cause the accumulation of the V(2) receptor intracellularly. Current studies are determining at which partial reaction in receptor synthesis, maturation, and surface delivery a particular mutation leads to V(2) receptor dysfunction; furthermore, we are utilizing mutant V(2) receptors as tools, in expression cloning strategies, to identify the molecules and machinery critical for G protein-coupled receptor delivery to the appropriate surface of polarized cells.

Publications
Tan, Christopher M, Brady, Ashley E, Nickols, Hilary Highfield, Wang, Qin, Limbird, Lee E. Membrane trafficking of G protein-coupled receptors. Annu Rev Pharmacol Toxicol, 44, 559-609, 2004

Brady, Ashley E, Wang, Qin, Colbran, Roger J, Allen, Patrick B, Greengard, Paul, Limbird, Lee E. Spinophilin stabilizes cell surface expression of alpha 2B-adrenergic receptors. J Biol Chem, 278(34), 32405-12, 2003

L?-mb?-rd, Lee E. Synergies at the synapse. Br J Pharmacol, 138(8), 1387-8, 2003 PMCID:1573812

Tan, Christopher M, Nickols, Hilary Highfield, Limbird, Lee E. Appropriate polarization following pharmacological rescue of V2 vasopressin receptors encoded by X-linked nephrogenic diabetes insipidus alleles involves a conformation of the receptor that also attains mature glycosylation. J Biol Chem, 278(37), 35678-86, 2003

Brady, A.E., and Limbird, L.E. G protein-coupled receptor interacting proteins: emerging roles in localization and signal transduction. Cell. Signalling, 14(4):397-309, 2002.

Feldman, R. and Limbird, L.E. Biochemical characterization of human adrenergic receptors. In: Human Adrenergic Receptors, P. Insel, editor, in press, 1984.

Tan, C.M., Wilson, M.H., MacMillan, L.B., Kobilka, B.K., Limbird, L.E. Heterozygous Alpha2A-adrenergic Receptor Mice Unveil Unique Therapeutic Benefits of Partial Agonists. In press, Proc. Natl. Acad. Sci., 2002.

Tan, C.M., and Limbird, L.E. Differential Sensitivity of the X-Linked Nephrogenic Diabetes Insipidus Mutant Alleles L292P, DV278, and R337X V2 Vasopressin Receptors to Surface and Functional Rescue. Submitted, 2001.

Wang, Q., and Limbird, L.E. Regulated Interactions of Spinophilin, 14-3-3z and Arrestin 3 with the a2A-adrenergic receptor. Submitted, 2001.

Brady, Ashley E, Limbird, Lee E. G protein-coupled receptor interacting proteins: emerging roles in localization and signal transduction. Cell Signal, 14(4), 297-309, 2002

Tan, Christopher M, Wilson, Matthew H, MacMillan, Leigh B, Kobilka, Brian K, Limbird, Lee E. Heterozygous alpha 2A-adrenergic receptor mice unveil unique therapeutic benefits of partial agonists. Proc Natl Acad Sci U S A, 99(19), 12471-6, 2002 PMCID:129469

Wang, Qin, Limbird, Lee E. Regulated interactions of the alpha 2A adrenergic receptor with spinophilin, 14-3-3zeta, and arrestin 3. J Biol Chem, 277(52), 50589-96, 2002

Wozniak, M., Saunders, C., Schramm, N., Keefer, J.R., Limbird, L.E. Morphological and biochemical strategies for monitoring trafficking of epitope-tagged G protein-coupled receptors in agonist-na?ve and agonist-occupied states. Methods Enzymol, 343(1), 530-544, 2002

Richman JG, Brady AE, Wang Q, Hensel JL, Colbran RJ, and Limbird LE. Agonist regulation of alpha(2)-adrenergic receptor interactions with spinophilin. J Biol Chem, 276, 15003-8, 2001, electronic publication, http://www.jbc.org/cgi/content/full/276/18/15003, May, 2001.

Richman, J G, Brady, A E, Wang, Q, Hensel, J L, Colbran, R J, Limbird, L E. Agonist-regulated Interaction between alpha2-adrenergic receptors and spinophilin. J Biol Chem, 276(18), 15003-8, 2001

Richman, J.G., Brady, A.E., Wang, Q., Hensel, J.L., Colbran, R.J., Limbird, L.E. Agonist regulation of a2 adrenergic receptor interactions with spinophilin. J Biol Chem, 276(18), 15003-15008, 2001

Schramm, N L, McDonald, M P, Limbird, L E. The alpha(2a)-adrenergic receptor plays a protective role in mouse behavioral models of depression and anxiety. J Neurosci, 21(13), 4875-82, 2001

Schramm, N.L., McDonald, M.P., and Limbird, L.E. The a2A-Adrenergic Receptor plays a protective role in mouse behavioral models of depression and anxiety. J Neurosci, 21(13), 4875-4882, 2001

Wilson, M H, Highfield, H A, Limbird, L E. The role of a conserved inter-transmembrane domain interface in regulating alpha(2a)-adrenergic receptor conformational stability and cell-surface turnover. Mol Pharmacol, 59(4), 929-38, 2001

Wilson, M.H., Highfield, H.A., Limbird, L.E. The role of a conserved inter-transmembrane domain interface in regulating a2A-adrenergic receptor conformational stability and cell-surface turnover. Mol Pharmacol, 59(4), 929-938, 2001

Edwards, S.W., Tan, C.M. and Limbird, L.E. Localization of G-protein-coupled receptors in health and disease. TiPS, 21(8), 304-308, 2000

Kingery, W.S., Guo, T.Z., Davies, M.F., Limbird, L.E., Maze, M. The a2A adrenoreceptor and the sympathetic postganglionic neuron contribute to the development of neuropathic heat hyperalgesia in mice. Pain, 85(3), 345-358, 2000

Lakhlani PP, MacMillan LB, Guo TZ, McCool BA, Lovinger DM, Maze M, Limbird LE. Substitution of a mutant alpha(2A)- adrenergic receptor via "hit and run" gene targeting reveals the role of this subtype in sedative, analgesic, and anesthetic-sparing responses in vivo. Proc Natl Acad Sci , 94, 9950-9955, 2000

McDuffie, J.E., Motley, E.D., Limbird, L.E., and Maleque, M.A. 5-hydroxytryptamine stimulates phosphorylation of p44/p42 mitogen-activated protein kinase activation in bovine aortic endothelial cell cultures. Journal of Cardiovascular Pharmacology, 35, 398-402, 2000

Saunders, C, Limbird, L E. Microtubule-dependent regulation of alpha(2B) adrenergic receptors in polarized MDCKII cells requires the third intracellular loop but not G protein coupling. Mol Pharmacol, 57(1), 44-52, 2000

Saunders, C., and Limbird, L. Microtubule-dependent regulation of a2B-adrenergic receptors in polarized MDCKII cells requires the third intracellular loop but not G-protein coupling. Mol. Pharm, 57(1), 44-52, 2000

Saunders, C., and Limbird, L.E. Colchicine evokes a subtype-selective increase in a2B-adrenergic receptor density on the surface MDCKII cells. Mol Pharmacol, 57(1), 44-52, 2000

Wilson MH and Limbird LE. Mechanisms regulating the cell surface residence time of the alpha(2A)-adrenergic receptor. Biochemistry, 39(4), 693-700, 2000

Wilson, M H, Limbird, L E. Mechanisms regulating the cell surface residence time of the alpha 2A-adrenergic receptor. Biochemistry, 39(4), 693-700, 2000

Wilson, M.H. and Limbird, L.E. Mechanisms regulating the cell surface residence time of the a2A-adrenergic receptor. Biochemistry , 39(4), 693-700, 2000

Wilson, M.H., and Limbird, L.E. Mechanisms regulating the cell surface residence time of the a2A-Adrenergic Receptor. Biochemistry, 39(4), 693-700, 2000

Altman JD, Trendelenburg AU, MacMillan L, Bernstein D, Limbird L, Starke K, Kobilka BK, Hein L. Abnormal regulation of the sympathetic nervous system in a2A-adrenergic receptor knockout mice. Mol Pharmacol, 56(1), 154-161, 1999

Edwards, S W, Limbird, L E. Role for the third intracellular loop in cell surface stabilization of the alpha2A-adrenergic receptor. J Biol Chem, 274(23), 16331-6, 1999

Edwards, S.W. and Limbird, L.E. Role for the third intracellular loop in cell surface stabilization of the a2A adrenergic receptor. J Biol Chem, 274(23), 16331-16336, 1999

Edwards, S.W. and Limbird, L.E. Role for the third intracellular loop in cell surface stabilization of the a2A adrenergic receptor. J. Biol. Chem., 274(23), 16331-16336, 1999

Guo, T., Davies, F., Kinery, W., Patterson, A., Limbird, L., Maze, M. Nitrous oxide produces antinociceptive response via a2B and/or a2C adrenoceptor subtypes in mice. Anesthesiology, 90(2), 470-6, 1999

Hein, L., Limbird, L.E., Eglen, R.M., Kobilka, B.K. Gene substitution/knockout to delineate the role of a2-adrenoceptor subtypes in mediating central effects of catecholamines and imidazolines. Ann NY Acad Sci, 881(1), 265-271, 1999

Limbird, L E, Vaughan, D E. Augmenting beta receptors in the heart: short-term gains offset by long-term pains. Proc Natl Acad Sci U S A, 96(13), 7125-7, 1999 PMCID:33583

Limbird, L. E. and Vaughan D. E. Augmenting beta receptors in the heart: short-term gains offset by long-term pains?. Proc. Natl. Acad. Sci., 96(13), 7125-7, 1999

Prezeau L, Richman JG, Edwards SW, and Limbird LE. The zeta isoform of 14-3-3 proteins interacts with the third intracellular loop of different alpha(2)-adrenergic receptor subtypes. J Biol Chem , 274(19), 13462-13469, 1999

Prezeau, L, Richman, J G, Edwards, S W, Limbird, L E. The zeta isoform of 14-3-3 proteins interacts with the third intracellular loop of different alpha2-adrenergic receptor subtypes. J Biol Chem, 274(19), 13462-9, 1999

Prezeau, L., Richman, J.G., Edwards, S.W., and Limbird, L.E. The z isoform of 14-3-3 proteins interacts with the third intracellular loop of different alpha2 adrenergic receptor subtypes. J Biol Chem, 274(19), 13462-13469, 1999

Saunders, C, Limbird, L E. Localization and trafficking of alpha2-adrenergic receptor subtypes in cells and tissues. Pharmacol Ther, 84(2), 193-205, 1999

Saunders, C., and Limbird, L. Localization and trafficking of a2-adrenergic receptor subtypes in cells and tissues. Pharmacology & Therapeutics, 84(2), 193-205, 1999

Schramm NL and Limbird LE. Stimulation of mitogen-activated protein kinase by G protein-coupled alpha(2)-adrenergic receptors does not require agonist-elicited endocytosis. J Biol Chem , 274(35), 24935-24940, 1999

Schramm, N L, Limbird, L E. Stimulation of mitogen-activated protein kinase by G protein-coupled alpha(2)-adrenergic receptors does not require agonist-elicited endocytosis. J Biol Chem, 274(35), 24935-40, 1999

Schramm, N. and Limbird, L.E. Stimulation of mitogen-activated protein kinase by G-protein-coupled a2-adrenergic receptors does not require agonist-elicited endocytosis. J Biol Chem, 274(35), 24935-24940, 1999

Janumpalli, S., Butler, L., MacMillan, L., Limbird, L., McNamara, J. A point mutation (D79N) of the a2A adrenergic receptor abolishes the antiepileptogenic action of endogenous norepinephrine. J Neurosci, 18(6), 2004-2008, 1998

Limbird, L E, Taylor, P. Endocrine disruptors signal the need for receptor models and mechanisms to inform policy. Cell, 93(2), 157-63, 1998

Limbird, L.E., Motulsky, H. Receptor identification and characterization. Handbook of Physiology, Oxford University Press, New York,, Chapter 4, 1998

MacMillan, L B, Lakhlani, P P, Hein, L, Piascik, M, Guo, T Z, Lovinger, D, Maze, M, Limbird, L E. In vivo mutation of the alpha 2A-adrenergic receptor by homologous recombination reveals the role of this receptor subtype in multiple physiological processes. Adv Pharmacol, 42, 493-6, 1998

MacMillan, L B, Lakhlani, P, Lovinger, D, Limbird, L E. Alpha 2-adrenergic receptor subtypes: subtle mutation of the alpha 2A-adrenergic receptor in vivo by gene targeting strategies reveals the role of this subtype in multiple physiological settings. Recent Prog Horm Res, 53, 25-42, 1998

MacMillan, L. B., Lakhlani, P., Lovinger, D., Limbird, L.E. In Vivo Mutation of the a2A-adrenergic receptor by homologous recombination reveals the role of this receptor subtype in multiple physiological processes. Advances in Pharmacology, Vol 42, 1998

Saunders, C, Keefer, J R, Bonner, C A, Limbird, L E. Targeting of G protein-coupled receptors to the basolateral surface of polarized renal epithelial cells involves multiple, non-contiguous structural signals. J Biol Chem, 273(37), 24196-206, 1998

Saunders, C., Keefer, J.R., Bonner, C.A., Limbird, L.E. Targeting of G-protein coupled receptors to the basolateral surface of polarized epithelia involves non-contiguous structural signals. J Biol Chem, 273(37), 24196-24206, 1998

Wilson, M H, Puranam, R S, Ottman, R, Gilliam, C, Limbird, L E, George, A L, McNamara, J O. Evaluation of the alpha(2A)-adrenergic receptor gene in a heritable form of temporal lobe epilepsy. Neurology, 51(6), 1730-1, 1998

Wilson, M. H. and Limbird, L.E. Evaluation of the a2A - adrenergic receptor gene as a candidate gene for an autosomal dominant form of temporal lobe epilepsy. Neurology, 51, 1730-1731, 1998

Wilson, M.H. and Limbird, L.E. Evaluation of the a2A-adrenergic receptor gene in a heritable form of temporal lobe epilepsy. Neurology, 51(6), 1730-1731, 1998

Wozniak, M, Limbird, L E. Trafficking itineraries of G protein-coupled receptors in epithelial cells do not predict receptor localization in neurons. Brain Res, 780(2), 311-22, 1998

Wozniak, M., and Limbird, L.E. Trafficking itineraries of G-protein coupled receptors in epithelial cells do not predict receptor localization in neurons. Brain Res, 780(2), 311-322, 1998

Lakhlani, P P, MacMillan, L B, Guo, T Z, McCool, B A, Lovinger, D M, Maze, M, Limbird, L E. Substitution of a mutant alpha2a-adrenergic receptor via 'hit and run' gene targeting reveals the role of this subtype in sedative, analgesic, and anesthetic-sparing responses in vivo. Proc Natl Acad Sci U S A, 94(18), 9950-5, 1997 PMCID:23306

Lakhlani, P., Macmillan, L., Guo, T.Z., McCool, B.A., Lovinger, D.M., Maze, M., and Limbird, L.E. Substitution of a mutant a2a-adrenergic receptor via ‘hit and run’ gene targeting reveals the role of this subtype in sedative, analgesic and anesthetic-sparing responses in vivo. Proc Natl Acad Sci USA, 94(18), 9950-9955, 1997

Saunders, C, Limbird, L E. Disruption of microtubules reveals two independent apical targeting mechanisms for G-protein-coupled receptors in polarized renal epithelial cells. J Biol Chem, 272(30), 19035-45, 1997

Saunders, C., Limbird, L.E. Disruption of microtubules reveals two independent apical targeting mechanisms for G-protein coupled receptors in polarized renal epithelial cells. J Biol Chem, 272(30), 19035-19045, 1997

Stone, L.S., MacMillan, L.B. Kitto, K.F., Limbird, L.E., Wilcox, G. The a2a-AR subtype mediates spinal, analgesia evoked by alpha2 agonists and is necessary for spinal adrenergic/opiod synergy. J Neurosci, 17(18), 7157-7165, 1997

Wang, R X, Limbird, L E. Distribution of mRNA encoding three alpha 2-adrenergic receptor subtypes in the developing mouse embryo suggests a role for the alpha 2A subtype in apoptosis. Mol Pharmacol, 52(6), 1071-80, 1997

Wang, R. and Limbird, L.E. Distribution of mRNA Encoding Three a2 Adrenergic Receptor Subtypes in the Developing Mouse Embryo Suggests a Role for the a2A Subtype in Apoptosis. Mol Pharmacol, 52(6), 1071-1080, 1997

Wozniak, M, Keefer, J R, Saunders, C, Limbird, L E. Differential targeting and retention of G protein-coupled receptors in polarized epithelial cells. J Recept Signal Transduct Res, 17(1-3), 373-83, 1997

Lakhlani, P P, Lovinger, D M, Limbird, L E. Genetic evidence for involvement of multiple effector systems in alpha 2A-adrenergic receptor inhibition of stimulus-secretion coupling. Mol Pharmacol, 50(1), 96-103, 1996

Lakhlani, P.P., Lovinger, D.M. and Limbird, L.E. Genetic evidence for involvement of multiple effector systems in a2A adrenergic receptor inhibition of stimulus-secretion coupling. Mol Pharmacol, 50(1), 96-103, 1996

MacMillan LB, Hein L, Smith MS, Piascik MT and Limbird LE. The alpha(2A)-adrenergic receptor subtype mediates central antihypertensive effects of alpha(2) agonist. Science, 273, 801-803, 1996

MacMillan, L B, Hein, L, Smith, M S, Piascik, M T, Limbird, L E. Central hypotensive effects of the alpha2a-adrenergic receptor subtype. Science, 273(5276), 801-3, 1996

MacMillan, L.B., Hein, L., Smith, M.S., Piascik, M.T. and Limbird, L.E. The a2-adrenoceptor subtype mediates central antihypertensive effects of a2-agonists. Science, 273(5276), 801-803, 1996

Saunders, C, Keefer, J R, Kennedy, A P, Wells, J N, Limbird, L E. Receptors coupled to pertussis toxin-sensitive G-proteins traffic to opposite surfaces in Madin-Darby canine kidney cells. A1 adenosine receptors achieve apical and alpha 2A adrenergic receptors achieve basolateral localization. J Biol Chem, 271(2), 995-1002, 1996

Saunders, C., Keefer, J.R., Kennedy, A.P., Wells, J.N. and Limbird, L.E. Receptors coupled to pertussis toxin-sensitive G-proteins traffic to opposite surfaces in Madin-Darby canine kidney cells . J Biol Chem, 271(2), 995-1002, 1996

Wang, R, Macmillan, L B, Fremeau, R T, Magnuson, M A, Lindner, J, Limbird, L E. Expression of alpha 2-adrenergic receptor subtypes in the mouse brain: evaluation of spatial and temporal information imparted by 3 kb of 5' regulatory sequence for the alpha 2A AR-receptor gene in transgenic animals. Neuroscience, 74(1), 199-218, 1996

Wang, R., MacMillan, L.B., Fremeau, R.T., Jr., Magnuson, M.A., Lindner, J. and Limbird, L.E. Expression of a2-adrenergic receptor subtypes in the mouse brain: evaluation of spatial and temporal information imparted by 3kb of 5' regulatory sequence for the a2A-AR-receptor gene in transgenic animals. Neuroscience, 74, 199-218, 1996

Wozniak M and Limbird LE. The three alpha(2)-adrenergic receptor subtypes achieve basolateral localization in Madin-Darby canine kidney II cells via different targeting mechanisms. J Biol Chem , 271, 5017-5024, 1996

Wozniak, M, Limbird, L E. The three alpha 2-adrenergic receptor subtypes achieve basolateral localization in Madin-Darby canine kidney II cells via different targeting mechanisms. J Biol Chem, 271(9), 5017-24, 1996

Limbird, L.E., MacMillan, L.B. and Keefer, J.R. Specificity in a2-adrenoreceptor signal transduction: receptor subtypes, coupling to distinct signal transduction pathways and localization to discrete subdomains in target cells. Pharmacology Communications, Special Issue: Pharmacology of Adrenoceptors. Norman G. Bowery and Robert R. Ruffulo, Jr. (Eds.) Harwood Academics Publishers, pp. 139-145, 1995

Uhlen, S., Axelrod, D., Keefer, J.R., Limbird, L.E. and Neubig, R.R. Membrane organization and mobility of a2-adrenergic receptors in MDCK cells. Adrenoceptors: Structure, Function and Pharmacology. R. Ruffolo, ed, 1995

Ceresa, B P, Limbird, L E. Mutation of an aspartate residue highly conserved among G-protein-coupled receptors results in nonreciprocal disruption of alpha 2-adrenergic receptor-G-protein interactions. A negative charge at amino acid residue 79 forecasts alpha 2A-adrenergic receptor sensitivity to allosteric modulation by monovalent cations and fully effective receptor/G-protein coupling. J Biol Chem, 269(47), 29557-64, 1994

Ceresa, B.P. and Limbird, L.E. Mutation of an aspartate residue highly conserved among G protein-coupled receptors results in non-reciprocal disruption of a2-adrenergic receptor-G protein. interactions. J Biol Chem, 269(47), 29557-29564, 1994

Chabre, O., Conklin, B.R., Brandon, S., Bourne, H.R. and Limbird, L.E. Coupling of the a2-adrenergic receptor to multiple G-proteins in a transient expression system. A simple approach for estimating receptor-G-protein coupling efficiency. J. Biol. Chem., 269, 5730-5734, 1994

Chabre, O., Conklin, B.R., Brandon, S., Bourne, H.R. and Limbird, L.E. Coupling of the a2-adrenergic receptor to multiple G-proteins in a transient expression system. A simple approach for estimating receptor G-protein coupling efficiency. J Biol Chem, 269(8), 5730-5734, 1994

Huang, J.J., Prakash, C., Womble, S.W., Wilson, A.L., Limbird, L.E. and Blair, I.A. Mass spectrometric approaches to the characterization of binding sites on adrenergic receptor proteins. Biological Mass Spectrometry: Present and Future. Matsuo, T., Caprioli, R.M., Gross, M.L. and Seyama, Y. (Eds). John Wiley & Sons, N.Y, pp 331-344, 1994

Keefer, J R, Kennedy, M E, Limbird, L E. Unique structural features important for stabilization versus polarization of the alpha 2A-adrenergic receptor on the basolateral membrane of Madin-Darby canine kidney cells. J Biol Chem, 269(23), 16425-32, 1994

Keefer, J R, Nunnari, J, Pang, I H, Limbird, L E. Introduction of purified alpha 2A-adrenergic receptors into uniformly oriented, unilamellar, phospholipid vesicles: productive coupling to G proteins but lack of receptor-dependent ion transport. Mol Pharmacol, 45(6), 1071-81, 1994

Keefer, J.F., Nunnari, J., Pang, I.H. and Limbird, L.E. Introduction of purified a2- adrenergic receptors into uniformly oriented, unilamellar phospholipid vesicles: Productive coupling to GTP-bindng proteins but lack of receptor-dependent ion transport. Molecular Pharmacology, 45, 1071-1081, 1994

Keefer, J.R., Kennedy, M.E. and Limbird, L.E. Unique structural features important for stabilization versus polarization of a2-adrenergic receptor on the basolateral receptor of Madin-Darby canine kidney cells. J Biol Chem, 269(23), 16425-16432, 1994

Kennedy, M E, Limbird, L E. Palmitoylation of the alpha 2A-adrenergic receptor. Analysis of the sequence requirements for and the dynamic properties of alpha 2A-adrenergic receptor palmitoylation. J Biol Chem, 269(50), 31915-22, 1994

Kennedy, M.E. and Limbird, L.E. Palmitoylation of the a2-adrenergic receptor: Analysis of the sequence requirements for and dynamic properties of a2-adrenergic receptor palmitoylation . J Biol Chem, 269(50), 31915-31922, 1994

Keefer, J R, Limbird, L E. The alpha 2A-adrenergic receptor is targeted directly to the basolateral membrane domain of Madin-Darby canine kidney cells independent of coupling to pertussis toxin-sensitive GTP-binding proteins. J Biol Chem, 268(15), 11340-7, 1993

Keefer, J.F. and Limbird, L.E. The a2-adrenergic receptor is targeted directly to the basolateral membrane domain of Madin-Darby canine kidney cells independent of coupling to pertussis toxin-sensitive GTP-binding proteins. J. Biol. Chem., 268, 11340-11347, 1993

Keefer, J.R. and Limbird, L.E. The a2-Adrenergic receptor is targeted directly to the basolateral membrane domains of Madin-Darby canine kidney cells independent of coupling to pertussis toxin-sensitive GTP-binding proteins. J Biol Chem, 268(15), 11340-11347, 1993

Kennedy, M E, Limbird, L E. Mutations of the alpha 2A-adrenergic receptor that eliminate detectable palmitoylation do not perturb receptor-G-protein coupling. J Biol Chem, 268(11), 8003-11, 1993

Kennedy, M.E. and Limbird, L.E. Mutations of the a2-adrenergic receptor that eliminate detectable palmitoylation do not perturb receptor-G-protein coupling. J. Biol. Chem., 268, 8003-8011, 1993

Kennedy, M.E. and Limbird, L.E. Mutations of the a2-adrenergic receptor that eliminate detachable palmitoylation do not perturb receptor-G-protein coupling. J Biol Chem, 268(11), 8003-8011, 1993

Domino, S.L., Repaske, M.G., Bonner, C.A., Kennedy, M.E., Wilson, A.L., Brandon, S. and Limbird, L.E. Synthesis of a yohimbine-agarose matrix useful for large-scale and micropurification of multiple a2 -receptor subtypes. Methods in Enzymology, Vol. 215 Platelets: Receptors, Adhesion, Secretion, Part B. Hawiger, J.J. (Ed); Academic Press, New York, pp 181-200, 1992

Surprenant, A., Horstman, D.A., Akbarali, H. and Limbird, L.E. A point mutation of the a2-adrenoceptor that blocks coupling to potassium but not calcium currents. Science, 257, 977-980, 1992

Surprenant, A., Horstman, D.A., Akbarali, H., and Limbird, L.E. A point mutation of the a2-adrenoceptor that blocks coupling to potassium but not calcium currents. Science, 257(5072), 977-980, 1992

Wilson, A L, Womble, S W, Prakash, C, Cragoe, E J, Blair, I A, Limbird, L E. Novel amiloride analog allosterically modulates the alpha 2-adrenergic receptor but does not inhibit Na+/H+ exchange. Mol Pharmacol, 42(2), 175-9, 1992

Wilson, A.L., Womble, S.W., Prakash, C., Cragoe, E.J., Jr., Blair, I.A. and Limbird, L.E. A novel amiloride analog allosterically modulates the a2-adrenergic receptor but does not inhibit Na+/H+ exchange. Mol. Pharmacol., 42, 175-179, 1992

Wilson, A.L., Womble, S.W., Prakash, C., Cragoe, E.J., Jr., Blair, I.A., and Limbird, L.E. A novel amiloride analog allosterically modulates the a2-adrenergic receptor but does not inhibit Na+/H+ exchange. Mol Pharmacol, 42(2), 175-179, 1992

Clark, J D, Limbird, L E. Na(+)-H+ exchanger subtypes: a predictive review. Am J Physiol, 261(6 Pt 1), C945-53, 1991

Clark, J.D. and Limbird, L.E. Na+/H+ exchanger subtypes: A predictive Review. Am. J. Physiology, 261, C945-C953, 1991

Horstman, D.A., Brandon, S., Wilson, A.L., Guyer, C.A. and Limbird, L.E. Identification of a single aspartate residue in the a2-adrenoceptor that confers sodium regulation of receptor-ligand interactions. Adrenoceptors: Structure, Mechanisms, Function. Advances in Pharmacological Sciences, Birkhauser Verlag Basel, pp. 149-158, 1991

Wilson, A L, Seibert, K, Brandon, S, Cragoe, E J, Limbird, L E. Monovalent cation and amiloride analog modulation of adrenergic ligand binding to the unglycosylated alpha 2B-adrenergic receptor subtype. Mol Pharmacol, 39(4), 481-6, 1991

Wilson, A.L., Seibert, K., Brandon, S., Cragoe, E.J., Jr. and Limbird, L. Monovalent cation and amiloride analog modulation of adrenergic ligand binding to the unglycosylated a2B-adrenergic receptor subtype. Mol. Pharmacol., 39, 481-486, 1991

Clark, J.D., Cragoe, Jr., E.J. and Limbird, L.E. a2-Adrenergic receptors regulate Na+, H+ exchange via a cAMP-dependent mechanism. Am. J. Physiol., 259, F977-F985, 1990

Clarke, J D, Cragoe, E J, Limbird, L E. Alpha 2-adrenergic receptors regulate Na(+)-H+ exchange via a cAMP-dependent mechanism. Am J Physiol, 259(6 Pt 2), F977-85, 1990

Guyer, C A, Horstman, D A, Wilson, A L, Clark, J D, Cragoe, E J, Limbird, L E. Cloning, sequencing, and expression of the gene encoding the porcine alpha 2-adrenergic receptor. Allosteric modulation by Na+, H+, and amiloride analogs. J Biol Chem, 265(28), 17307-17, 1990

Guyer, C.A., Horstman, D.A., Wilson, A.L., Clark, J.D., Cragoe, Jr., E.J. and Limbird, L.E. Cloning, sequencing and expression of the gene encoding the porcine a2-adrenergic receptor. Allosteric modulation by Na+, H+ and amiloride analogs. J. Biol. Chem. , 265, 17307-17317, 1990

Guyer, C.A., Horstman, D.A., Wilson, A.L., and Limbird, L.E. Cloning sequencing and expression of the gene encoding the porcine alpha2-adrenergic receptor: Allosteric modulation by Na+, H+ and amiloride analogs. J Biol Chem, 265(28), 17307-17317, 1990

Horstman, D A, Brandon, S, Wilson, A L, Guyer, C A, Cragoe, E J, Limbird, L E. An aspartate conserved among G-protein receptors confers allosteric regulation of alpha 2-adrenergic receptors by sodium. J Biol Chem, 265(35), 21590-5, 1990

Horstman, D.A., Brandon, S., Wilson, A.L., Guyer, C.A., Cragoe, Jr., E.J. and Limbird, L.E. An aspartate conserved among G-protein-coupled receptors confers allosteric regulation of a2-adrenergic receptors by sodium. J. Biol. Chem., 265, 21590-21595, 1990

Horstman, D.A., Brandon, S., Wilson, A.L., Guyer, C.A., Cragoe, Jr., E.J., and Limbird, L.E. An aspartate conserved among G-protein coupled receptors confers allosteric regulation of a2-adrenergic receptors by sodium. J Biol Chem, 265(35), 21590-21595, 1990

Wilson, A L, Guyer, C A, Cragoe, E J, Limbird, L E. The hydrophobic tryptic core of the porcine alpha 2-adrenergic receptor retains allosteric modulation of binding by Na+, H+, and 5-amino-substituted amiloride analogs. J Biol Chem, 265(28), 17318-22, 1990

Wilson, A.L., Guyer, C.A., Cragoe, Jr., E.J. and Limbird, L.E. The hydrophobic tryptic core of the porcine a2-adrenergic receptor retains allosteric modulation of binding by Na+, H+ and 5-amino substituted analogs of amiloride. J. Biol. Chem, 265, 17318-17322, 1990

Wilson, A.L., Guyer, C.A., Cragoe, Jr., E.J., and Limbird, L.E. The hydrophobic tryptic core of the porcine a2-adrenergic receptor retains allosteric modulation of binding by Na+, H+ and 5-amino substituted amiloride analogs. J Biol Chem, 265(28), 17318-17322, 1990

Beebe, S J, Segaloff, D L, Burks, D, Beasley-Leach, A, Limbird, L E, Corbin, J D. Evidence that cyclic adenosine 3',5'-monophosphate-dependent protein kinase activation causes pig ovarian granulosa cell differentiation, including increases in two type II subclasses of this kinase. Biol Reprod, 41(2), 295-307, 1989

Limbird, L.E. Hormonal inhibition of adenylate cyclase: A possible mechanism for physiological antagonism. Antihormones, M.K. Agarwal, editor, 661-669, 1989

Segaloff, D.L., Beebe, S.J., Corbin, J.D. and Limbird, L.E. LH-receptor induction and progesterone biosynthesis in porcine granulosa cells: The use of cAMP analogs to demonstrate the role of the cAMP dependent protein kinase in hormone-mediated differentiation. Biol. of Receptors, 41, 295-307, 1989

Baron, B M, Limbird, L E. Human platelet phospholipase A2 activity is responsive in vitro to pH and Ca2+ variations which parallel those occurring after platelet activation in vivo. Biochim Biophys Acta, 971(1), 103-11, 1988

Baron, B.M. and Limbird, L.E. Human platelet phospholipase a2 activity in response in vitro to pH and Ca2+ variations which parallel those occurring after platelet activation in vivo. Biochem. Biophys. Acta, 971, 103-111, 1988

Esser V, Limbird LE, Brown MS, Goldstein JL, and Russell DW. Mutational Analysis of the Ligand Binding Domain of the Low Density Lipoprotein Receptor . J Biol Chem, 263(26), 13282-13291, 1988

Esser, V., Limbird, L.E., Brown, M.S., Goldstein, J.L. and Russell, D.W. Mutational analysis of the ligand binding domain of the low density lipoprotein receptor. J. Biol. Chem., 263, 13282-13291, 1988

Guyer, C.A. and Limbird, L. Alpha2-adrenergic receptors and Na+/H+ exchange. Platelet and Vascular Occlusion, G.A. FitzGerald, C. Patrono, editors. Raven Press Serono Symposia Publications, 54, 161-180, 1988

Isom, L.L. and Limbird, L.E. What happens next?: An hypothesis linking the biochemical and electrophysiological sequelae of a2-adrenergic receptor occupancy with their diverse physiological consequences. Alpha2-Adrenergic Receptors, L.E. Limbird, editor, D. Bylund, series editor, Humana Press, Chapter 6, pp. 323-363, 1988

Limbird, L E. Receptors linked to inhibition of adenylate cyclase: additional signaling mechanisms. FASEB J, 2(11), 2686-95, 1988

Limbird, L.E. Receptors linked to inhibition of adenylate cyclase: Alternative signaling mechanisms. FASEB J, 2(11), 2686-2695, 1988

Sweatt, J D, Connolly, T M, Baron, B M, Limbird, L E. Involvement of Na+/H+ exchange in human platelet activation. Prog Clin Biol Res, 283, 523-57, 1988

Sweatt, J.D., Connolly, T.M., Baron, B.M. and Limbird, L.E. Involvement of Na+/H+ exchange in human platelet activation. Progress in Clinical and Biological Research, 283, 523-557, 1988

Isom, L L, Cragoe, E J, Limbird, L E. Multiple receptors linked to inhibition of adenylate cyclase accelerate Na+/H+ exchange in neuroblastoma x glioma cells via a mechanism other than decreased cAMP accumulation. J Biol Chem, 262(36), 17504-9, 1987

Isom, L.L., Cragoe, E.J., Jr. and Limbird, L.E. Multiple receptors linked to inhibition of adenylate cyclase accelerate Na+/H+ exchange in neuroblastoma x glioma cells via a mechanism other than decreased cAMP accumulation. J. Biol. Chem., 262, 17504-17509, 1987

Isom, L.L., Cragoe, E.J., Jr. and Limbird, L.E. a2-adrenergic receptors accelerate Na+/H+ exchange in neuroblastoma x glioma cells. J. Biol. Chem., 262(14), 6750-6767, 1987

Nunnari, J.M., Repaske, M.G., Brandon, S. and Limbird, L.E. Modulation of a2- adrenergic receptor-ligand interactions by Na+, H+ and inhibitors of Na+/H+ exchange: Implications for interactions between the alpha2-adrenergic receptor and a Na+/H+ antiporter. J. Biol. Chem. , 262(25), 12387-12392, 1987

Repaske, M.G., Nunnari, J.M. and Limbird, L.E. Purification of the a2-adrenergic receptor from porcine brain using a yohimbine-agarose affinity matrix. J. Biol. Chem., 262(25), 12381-12386, 1987

Sweatt, J D, Schwartzberg, M S, Frazer, M, Cragoe, E J, Blair, I A, Reed, P W, Limbird, L E. Evidence for a role for Na+-H+ exchange in activation of human platelets by PAF. Circ Res, 61(5 Pt 2), II6-11, 1987

Sweatt, J.D., Schwartzberg, M.S., Frazer, M., Cragoe, E. J., Blair, I.A., Reed, P. C., and Limbird, L.E. Evidence for a role for Na+/H+ exchange in activation of human platelets by PAF. Circulation Research, 61(5), 116-1111, 1987

Sweatt, J.D., Schwartzberg, M.S., Frazer, M., Cragoe, E.J. Jr., Blair, I.A., Reed, P.W. and Limbird, L.E. Evidence of a role for Na+/H+ exchange in activation of human platelets by PA. Circ. Res. Suppl. (Part II), 61, 6-11, 1987

Beebe, S.J., Blackmore, P.F., Koch, S.R., Granner, D.K., Segaloff, D.L., Burks, D., Limbird, L.E. and Corbin, J.D. The use of cAMP analogs to study cAMP-dependent protein kinase mediated events in intact mammalian cells. Hormones and Cell Regulation, 139, 159-180, 1986

Lanier, S.M., Graham, R.M., Hess, H.J., Grodski, A., Repaske, M.G., Nunnari, J.M., Limbird, L.E. and Homcy, C.J. Photoaffinity labeling of the porcine brain a2-adrenergic receptor using a radio-iodinated arylazide derivative of rauwolscine: Identification of the hormone binding subunit. Proc. Natl. Acad. Sci. USA, 83, 1986

Sweatt, J.D., Blair, I., Cragoe, E.J., Jr. and Limbird, L.E. Inhibitors of Na+/H+ exchange block epinephrine and ADP-induced stimulation of human platelet phospholipase C by blockade of arachidonic acid release at a prior step. J. Biol. Chem, 261, 8660-8666, 1986

Sweatt, J.D., Connolly, T.M., Cragoe, E.J., Jr. and Limbird, L.E. Evidence that Na+/H+ exchange regulates receptor-mediated phospholipase a2 activation in human platelets. J. Biol. Chem. , 261, 8667-8673, 1986

Limbird, L.E. and Connolly, T.M. Studies of the molecular basis for regulation of human platelet adenylate cyclase and platelet activation by a2-adrenergic receptors. Interactions of Platelets with the Vessel-Wall, American Physiological Society, 1985

Limbird, L.E. and Sweatt, J.D. a2-adrenergic receptors: apparent interaction with multiple effector systems. The Receptors, P.M. Conn, editor, Academic Press II, 281-305, 1985

Limbird, L.E., Connolly, T.M. and Sweatt, J.D. The human platelet alpha2-adrenergic receptor system: The role of Na+ in epinephrine-receptor interactions, arachidonic acid release and Ca++ mobilization. The Pharmacology of Adrenoceptors, E. Szabadi, C.M. Bradshaw and S.R. Nahorski, editors, MacMillan Press, Ltd, pp. 49-58, 1985

Limbird, L.E., Connolly, T.M., Sweatt, J.D. and Uderman, H.D. Human platelet a2- adrenergic receptors: Effect of Na+ on interaction with the adenylate cyclase system and on epinephrine-stimulated platelet secretion. Advances in Cyclic Nucleotide Research, 19, 235-242, 1985

Limbird, L.E., MacMillan, S.T. and Kalinoski, D.L. The resolution of agonist-alpha2- adrenergic receptor complexes from unoccupied receptors or antagonist- a2- receptor complexes using DEAE chromatography. J. Cycl. Nuc. and Prot. Phos. Res, 10, 75-82, 1985

Sweatt, J.D., Johnson, S.L., Cragoe, E.J. and Limbird, L.E. Evidence that a Na+/H+ exchange mechanism controls stimulus-provoked arachidonic acid release in human platelets. J. Biol. Chem., 260, 12910-12919, 1985

. The role of sodium in epinephrine-provoked arachidonic acid release and dense granule secretion from human platelets. Symposia Medica Hoechst, 553-575, 1985

Connolly, T.M., Uderman, H.D. and Limbird, L.E. Removal of extra platelet Na+ blocks stimulus-provoked arachidonic acid release and diminishes stimulus-provoked Ca++ availability. Ion Transport Regulation Vol. 1, Prostaglandins and Membrane Ion Transport. P. Braquet, R.P. Garay, G.C. Frohlich and S. Nicosia, editors, pp. 51-56, 1984

Feldman, R., Limbird, L.E., Nadeau, J., Robertson, D. and Wood, A.J.J. Leukocyte b- receptor alterations in hypertensive subjects. Lancet, 73, 648-653, 1984

Feldman, R.D., Limbird, L.E., Nadeau, J., Robertson, D. and Wood, A.J.J. Alterations in leukocyte beta-receptor affinity with aging: A potential explanation for altered beta-adrenergic sensitivity in the elderly. New Engl. J. Med., 310, 815-819, 1984

Limbird, L.E. GTP and Na+ modulate receptor adenylate cyclase coupling and receptor- mediated function. Invited review for Amer. J. Physiol. (Endocrinology and Metabolism, 10), 247, E59-E68, 1984

Segaloff, D.L., May, J.V., Schomberg, D.W. and Limbird, L.E. LH/hCG receptor induction in primary cultures of porcine granulosa cells. Biochem. Biophys. Acta , 31-36, 1984

Beta-adrenergic activation and alpha-adrenergic inhibition of adenylate cyclase: GTP-binding proteins as macromolecular messengers. Limbird, L.E. Adv. Exp. Med., 161, 91- 111, 1983

Connolly, T.M. and Limbird, L.E. Removal of extra platelet Na+ eliminates indomethacin- sensitive secretion from human platelet stimulated by epinephrine, ADP and thrombin. Proc. Natl. Acad. Sci. USA, 80, 5320-5324, 1983

Connolly, T.M. and Limbird, L.E. The influence of Na+ on the a2-adrenergic receptor- adenylate cyclase system of human platelets. I. A method for removal of extra platelet Na+. Effect of Na+ removal on aggregation, secretion and cAMP accumulation. J. Biol. Chem., 258, 3907-3912, 1983

Feldman, R., Limbird, L.E., Nadeau, J., FitzGerald, G.A., Robertson, D. and Wood, A.J.J. Dynamic regulation of leukocyte beta-adrenergic receptor-agonist interactions by physiological changes in circulating catecholamines. J. Clin. Invest., 72, 164-170, 1983

Limbird, L.E. Adrenergic receptors and regulation of adenylate cyclase activity: Methodological approaches and interpretation of data in terms of receptor-cyclase coupling. Principles of Recepterology, M.K. Agarwal, editor. W. DeGruyter, publisher, pp. 593-628, 1983

Limbird, L.E. a2-Adrenergic systems. exploring hormonal inhibition of adenylate cyclase. Trends in Pharmacological Sciences, 4, 135-138, 1983

Limbird, L.E. and Speck, J.L. N-ethylmaleimide, temperature and digitonin solubilization eliminate guanine nucleotide but not Na+ effects on human platelet a2-adrenergic receptor- agonist interactions. J. Cyclic Nucleotide and Protein Phosphorylation Research, 9, 191- 202, 1983

Limbird, L.E., Buhrow, S.A., Speck, J.L. and Staros, J.V. 5'-p-fluoro-sulfonylbenzoyl guanine as a probe for the GTP-binding protein in a2-adrenergic receptor-adenylate cyclase systems. J. Biol. Chem., 258, 10289-10293, 1983

Segaloff, D.L. and Limbird, L.E. Luteinizing hormone receptor appearance in cultured porcine granulosa cells requires the continued presence of follicle-stimulating hormone. Proc. Natl. Acad. Sci. USA , 80, 5631-5636, 1983

Segaloff, D.L. and Limbird, L.E. The cAMP-dependent FSH induction of LH receptors in primary cultures of porcine granulosa cells is not due to the expression of an intracellular pool of LH receptors. Endocrinology, 113, 825-827, 1983

Limbird, L.E., Speck, J.L. and Smith, S.K. Sodium ion modulates agonist and antagonist interactions with the human platelet a2-adrenergic receptor in membrane and solubilized preparations. Mol. Pharmacol., 41, 607-619, 1982

Smith, S.K. and Limbird, L.E. Apparent independence of the alpha-adrenergic system of the human platelet from the cholera toxin-catalyzed ADP-ribosylated 42,000 Mr subunit of the adenylate cyclase system. J. Biol. Chem., 257, 10471-10478, 1982

Lefkowitz, R.J., DeLean, A., Hoffman, B.B., Stadel, J.M., Kent, R., Michel, T. and Limbird, L.E. Molecular pharmacology of the adenylate cyclase-coupled - a and b-adrenergic receptors. Adv. in Cyclic Nucleotide Res, 14, 145-162, 1981

Limbird, L.E. Activation and attenuation of adenylate cyclase: GTP-binding proteins as macromolecular messengers in receptor-cyclase coupling. Biochem. J, 195, 1-13, 1981

Limbird, L.E. and MacMillan, S.T. Mn++ uncoupling of the catecholamine-sensitive adenylate cyclase system of reticulocytes. Parallel effects of cholera toxin-catalyzed ADP-ribosylation on the system. Biochem. Biophys. Acta., 677, 408-416, 1981

Limbird, L.E., MacMillan, S.T. and Smith, S.K. Solubilization of human platelet a2 adrenergic receptors: Evidence for agonist-promoted receptor-effector association. Advances in Cyclic Nucleotide Res, 14, 189-198, 1981

Smith, S.K. and Limbird, L.E. Solubilization of human platelet a-adrenergic receptors: Evidence that agonist occupancy of the receptors stabilizes receptor-effector interactions. Proc. Natl. Acad. Sci., 78, 4026-4030, 1981

Stadel, J.M., Schorr, R.G.L., Limbird, L.E. and Lefkowitz, R.J. Evidence that a beta- adrenergic receptor-associated guanine nucleotide regulatory protein conveys GTP-*S Dependent adenylate cyclase activity. J. Biol. Chem, 256, 8718-8723, 1981

Limbird, L.E., Gill, D.M. and Lefkowitz, R.J. Agonist-promoted coupling of the beta- adrenergic receptor with the guanine nucleotide regulatory protein of the adenylate cyclase system. Proc. Natl. Acad. Sci., 77, 775-779,, 1980

Limbird, L.E., Gill, D.M., Stadel, J.M., Hickey, A.R. and Lefkowitz, R.J. Loss of b adrenergic receptor-guanine nucleotide regulatory protein interactions accompanies decline in catecholamine responsiveness of adenylate cyclase in maturing rat erythrocytes. J. Biol. Chem., 255, 1854-1861, 1980

Caron, M.G., Limbird, L.E. and Lefkowitz, R.J. Biochemical characterization of the beta- adrenergic receptor of the frog erythrocyte. Molecular and Cellular Biochemistry, 28, 45-66, 1979

Limbird, L.E., DeLean, A., Hickey, A.R., Pike, L.J. and Lefkowitz, R.J. Differential effects of GTP on the coupling of beta-adrenergic receptors to adenylate cyclase from frog and turkey erythrocytes: Application of new graphic methods for the analysis of receptor-effector coupling. Biochem. Biophys. Acta, 586, 298-314, 1979

Limbird, L.E., Hickey, A.R. and Lefkowitz, R.J. The molecular size of adenylate cyclase in the presence and absence of hormone and guanine nucleotide effectors. J. Cyclic Nuc. Res, 5, 251-259, 1979

Limbird, L.E., Hickey, A.R. and Lefkowitz, R.J. Unique uncoupling of the frog erythrocyte adenylate cyclase system by manganese. Lose of hormone and guanine nucleotide-sensitive enzyme activities without loss of nucleotide-sensitive, high affinity agonist binding. J. Biol. Chem., 254, 2677-2683, 1979

Pike, L.J., Limbird, L.E. and Lefkowitz, R.J. Beta-adrenergic receptors determine affinity but not intrinsic activity of drugs for stimulation of adenylate cyclase. Nature, 280, 502-504, 1979

Lefkowitz, R.J., Limbird, L.E., Williams, L.T. and Wessels, M. Beta-adrenergic receptors: Regulatory role of agonists. J. Supra-Molecular Structure, 8, 501-510, 1978

Limbird, L.E. and Lefkowitz, R.J. Beta-adrenergic receptors: Agonist induced increase in apparent molecular size. Proc. Natl. Acad. Sci. USA, 75, 228-232, 1978

Limbird, L.E. and Lefkowitz, R.J. Resolution of beta-adrenergic receptor binding and adenylate cyclase activity by gel exclusion chromatography. J. Biol. Chem. , 252, 799-802, 1977

Jarmakani, J.M., Limbird, L.E., Graham, T. and Marks, R.A. Effect of reperfusion on myocardial infarct and the accuracy of estimating infarct size from serum creatine phosphokinase in the dog. Cardiovascular Research, 10, 245-253,, 1976

Lefkowitz, R.J. and Limbird, L.E. Biochemical techniques for the study of drug action. Progress in Cardiovascular Diseases, 18, 309-321, 1976

Lefkowitz, R.J., Caron, M.G., Limbird, L.E., Mukherjee, C. and Williams, L.T. "Membrane- Bound Receptors". The Enzymes of Biological Membranes, A. Martonosi, editor, pp. 283- 310, 1976

Lefkowitz, R.J., Limbird, L.E., Mukherjee, C. and Caron, M.G. The beta-adrenergic receptor and adenylate cyclase. Biomembrane Reviews (Biochem. Biophys. Acta), 457, 1- 39, 1976

Lefkowitz, R.J., Mukherjee, C., Limbird, L.E., Caron, M.G., Williams, L.T., Mickey, J.V. and Tate, R. Regulation of adenylate cyclase-coupled beta-adrenergic receptors. Recent Progress in Hormone Research, 32, 597-632, 1976

Limbird, L.E. Receptors linked to inhibition of adenylate cyclase: Alternative signaling mechanisms. FASEB J. , 2, 2686-2695, 1976

Limbird, L.E. and Lefkowitz, R.J. Negative cooperativity among beta-adrenergic receptors. J. Biol. Chem., 251, 5007-5014, 1976

Limbird, L.E. and Lefkowitz, R.J. Biochemical and molecular characteristics of beta- adrenergic receptor binding sites. In: Proceedings of the NATO Advanced Study Institute on Surface Membrane Receptors. NATO ASI Series, 11, 387-404, 1976

Limbird, L.E. and Lefkowitz, R.J. Adenylate cyclase-coupled beta-adrenergic receptors: Effect of membrane lipid-perturbing agents on receptor binding and enzyme stimulation by catecholamines. Molecular Pharmacology, 12, 559-567, 1976

Limbird, L.E. and Lefkowitz, R.J. Myocardial guanylate cyclase: Properties of the enzyme and effects of cholinergic agonists in vitro. Biochem. Biophys. Acta. (Enzymology), 377, 185- 196, 1975

Limbird, L.E., DeMeyts, P. and Lefkowitz, R.J. Beta-adrenergic receptors: Evidence for negative cooperativity. Biochem. Biophys. Res. Commun., 64, 1160-1168,, 1975

Development of a Method for the Detection and Quantitation of the Isoenzymes of Creatine Phosphokinase and the Application of Combined Creatine Phosphokinase and Lactate Dehydrogenase Isoenzyme Analysis to the Recognition of Acute Myocardial Infarction. Ph.D. Thesis, 1973.

Dixon, S.J., Limbird, L.E., Roe, C.R., Wagner, G.S., Oldham, N.H. and Sabiston, D.C. Recognition of post-operative myocardial infarction. Circulation, 48, 137, 1973

Wagner, G.S., Roe, C.R., Limbird, L.E., Rosati, P.A. and Wallace, A.G. The importance of identification of the myocardial specific isoenzyme of creatine phosphokinase (MB form) in the diagnosis of acute myocardial infarction. Circulation, 47, 263, 1973

. Roeyme analysis in the diagnosis of myocardial injury: Application of electrophoretic methods for the detection and quantitation of the creatine phosphokinase MB isoenzyme. J. Lab. and Clin. Med., 80, 577, 1972


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