Biomedical Research Education & Training
Faculty Member

Marnett, Lawrence J., Ph.D.
University Professor of Biochemistry and Chemistry
Mary Geddes Stahlman Chair in Cancer Research
Professor of Pharmacology
Professor of Chemistry

Lab Url: http://bret.mc.vanderbilt.edu/marnett

Phone Number: 615-343-7329

Email Address: larry.marnett@vanderbilt.edu

Marnett, Lawrence's picture
Academic history
B.S., Rockhurst College, Kansas City KS
Ph.D., Duke University, Durham NC
Postdoc, Karolinska Institute, Sweden
Postdoc, Wayne State University, Detroit MI

Office Address   Mailing Address

850 Robinson Research Building

850 RRB 0146


Research Keywords
cyclooxygenase, prostaglandins, antiinflammatory agent, endocannabinoids, DNA damage, mutagenesis, oxidative stress and cell signaling, apoptosis

Research Specialty
Cyclooxygenase-2 structure and function; chemistry and biology of DNA damage-dependent mutagenesis and signaling

Research Description
A major interest in the Marnett laboratory is the structure and function of cyclooxygenase-2 (COX-2). This enzyme catalyzes the committed step in the production of prostaglandins and is the molecular target for non-steroidal antiinflammatory drugs. Functional analysis of COX-2 by structure-guided mutagenesis has uncovered new strategies for synthesizing COX-2 inhibitors and has suggested new biological roles for the enzyme. Current work focuses on the generation of novel antiinflammatory drugs with additional pharmacological functions and on investigations of the chemistry and biology of COX-2-dependent oxygenation of the endocannabinoid, 2-arachidonylglycerol, to glyceryl prostaglandins.

Another major interest in the Marnett laboratory is the chemistry and biology of DNA damage by endogenously generated products of oxidative stress. Individual products of DNA damage are incorporated into viral genomes for in vivo mutagenesis experiments. Similar experiments are used to identify human DNA repair enzymes that remove specific forms of DNA damage.

A new area of interest in the laboratory is the investigation of protein targets of drugs and toxic compounds and the consequences of protein modification in modulating biological function.

A broad range of techniques are used by the Marnett group including chemical synthesis, enzymology, protein expression, site-directed mutagenesis, bioanalytical chemistry, mass spectrometry, in vivo mutagenesis, tissue culture, proteomics, and techniques of signal transduction.


Publications
Bala, M, Chin, CN, Logan, AT, Amin, T, Marnett, LJ, Boutaud, O, Oates, JA. Acetylation of prostaglandin H2 synthases by aspirin is inhibited by redox cycling of the peroxidase. Biochem Pharmacol, 75(7), 1472-81, 2008 PMCID:2693035

Felts, AS, Siegel, BS, Young, SM, Moth, CW, Lybrand, TP, Dannenberg, AJ, Marnett, LJ, Subbaramaiah, K. Sulindac Derivatives That Activate the Peroxisome Proliferator-activated Receptor gamma but Lack Cyclooxygenase Inhibition. J Med Chem, 2008 PMCID:2651753

Ghidu, VP, Wang, J, Wu, B, Liu, Q, Jacobs, A, Marnett, LJ, Sulikowski, GA. Synthesis and evaluation of the cytotoxicity of apoptolidinones A and D. J Org Chem, 73(13), 4949-55, 2008 PMCID:2572754

Knutson, CG, Skipper, PL, Liberman, RG, Tannenbaum, SR, Marnett, LJ. Monitoring in Vivo Metabolism and Elimination of the Endogenous DNA Adduct, M1dG {3-(2-Deoxy-beta-d-erythro-pentofuranosyl)pyrimido[1,2-alpha]purin-10(3H)-one}, by Accelerator Mass Spectrometry. Chem Res Toxicol, 2008

Rouzer, CA, Marnett, LJ. Non-redundant functions of cyclooxygenases: oxygenation of endocannabinoids. J Biol Chem, 283(13), 8065-9, 2008 PMCID:2417164

Stafford, JB, Marnett, LJ. Prostaglandin E2 inhibits tumor necrosis factor-alpha RNA through PKA type I. Biochem Biophys Res Commun, 366(1), 104-9, 2008

Szekely, J, Rizzo, CJ, Marnett, LJ. Chemical properties of oxopropenyl adducts of purine and pyrimidine nucleosides and their reactivity toward amino acid cross-link formation. J Am Chem Soc, 130(7), 2195-201, 2008 PMCID:2708936

Szekely, J, Wang, H, Peplowski, KM, Knutson, CG, Marnett, LJ, Rizzo, CJ. "One-pot" syntheses of malondialdehyde adducts of nucleosides. Nucleosides Nucleotides Nucleic Acids, 27(2), 103-9, 2008

Turman, MV, Kingsley, PJ, Rouzer, CA, Cravatt, BF, Marnett, LJ. Oxidative metabolism of a fatty acid amide hydrolase-regulated lipid, arachidonoyltaurine. Biochemistry, 47(12), 3917-25, 2008

Vanderveen, LA, Harris, TM, Jen-Jacobson, L, Marnett, LJ. Formation of DNA-Protein Cross-Links Between gamma-Hydroxypropanodeoxyguanosine and EcoRI. Chem Res Toxicol, 2008 PMCID:2651693

Vila, A, Tallman, KA, Jacobs, AT, Liebler, DC, Porter, NA, Marnett, LJ. Identification of protein targets of 4-hydroxynonenal using click chemistry for ex vivo biotinylation of azido and alkynyl derivatives. Chem Res Toxicol, 21(2), 432-44, 2008

Wang, Y, Musser, SK, Saleh, S, Marnett, LJ, Egli, M, Stone, MP. Insertion of dNTPs opposite the 1,N2-propanodeoxyguanosine adduct by Sulfolobus solfataricus P2 DNA polymerase IV. Biochemistry, 47(28), 7322-34, 2008 PMCID:2753402

Blobaum, AL, Marnett, LJ. Molecular determinants for the selective inhibition of Cyclooxygenase-2 by Lumiracoxib. J Biol Chem, 2007

Blobaum, AL, Marnett, LJ. Structural and functional basis of cyclooxygenase inhibition. J Med Chem, 50(7), 1425-41, 2007

Druckova, A, Mernaugh, RL, Ham, AJ, Marnett, LJ. Identification of the protein targets of the reactive metabolite of teucrin A in vivo in the rat. Chem Res Toxicol, 20(10), 1393-408, 2007

Jacobs, AT, Marnett, LJ. Heat shock factor 1 attenuates 4-Hydroxynonenal-mediated apoptosis: critical role for heat shock protein 70 induction and stabilization of Bcl-XL. J Biol Chem, 282(46), 33412-20, 2007

Joo, M, Kwon, M, Sadikot, RT, Kingsley, PJ, Marnett, LJ, Blackwell, TS, Peebles, RS, Urade, Y, Christman, JW. Induction and function of lipocalin prostaglandin D synthase in host immunity. J Immunol, 179(4), 2565-75, 2007

Kingsley, PJ, Marnett, LJ. LC-MS-MS analysis of neutral eicosanoids. Methods Enzymol, 433, 91-112, 2007

Knutson, CG, Akingbade, D, Crews, BC, Voehler, M, Stec, DF, Marnett, LJ. Metabolism in vitro and in vivo of the DNA base adduct, M1G. Chem Res Toxicol, 20(3), 550-7, 2007

Knutson, CG, Wang, H, Rizzo, CJ, Marnett, LJ. Metabolism and elimination of the endogenous DNA adduct, 3-(2-deoxy-beta-D-erythropentofuranosyl)-pyrimido[1,2-alpha]purine-10(3H)-one, in the rat. J Biol Chem, 282(50), 36257-64, 2007

Prusakiewicz, JJ, Turman, MV, Vila, A, Ball, HL, Al-Mestarihi, AH, Di Marzo, V, Marnett, LJ. Oxidative metabolism of lipoamino acids and vanilloids by lipoxygenases and cyclooxygenases. Arch Biochem Biophys, 464(2), 260-8, 2007

Rouzer, CA, Ivanova, PT, Byrne, MO, Brown, HA, Marnett, LJ. Lipid Profiling Reveals Glycerophospholipid Remodeling in Zymosan-Stimulated Macrophages. Biochemistry, 2007

Vila, A, Rosengarth, A, Piomelli, D, Cravatt, B, Marnett, LJ. Hydrolysis of prostaglandin glycerol esters by the endocannabinoid-hydrolyzing enzymes, monoacylglycerol lipase and fatty acid amide hydrolase. Biochemistry, 46(33), 9578-85, 2007

Wang, H, Xie, H, Sun, X, Kingsley, PJ, Marnett, LJ, Cravatt, BF, Dey, SK. Differential regulation of endocannabinoid synthesis and degradation in the uterus during embryo implantation. Prostaglandins Other Lipid Mediat, 83(1-2), 62-74, 2007 PMCID:1805469

Wang, Y, Schnetz-Boutaud, NC, Saleh, S, Marnett, LJ, Stone, MP. Bulge migration of the malondialdehyde OPdG DNA adduct when placed opposite a two-base deletion in the (CpG)3 frameshift hotspot of the Salmonella typhimurium hisD3052 gene. Chem Res Toxicol, 20(8), 1200-10, 2007 PMCID:2728581

Druckova, A, Marnett, LJ. Characterization of the amino acid adducts of the enedial derivative of teucrin A. Chem Res Toxicol, 19(10), 1330-40, 2006

Muthian, G, Raikwar, HP, Johnson, C, Rajasingh, J, Kalgutkar, A, Marnett, LJ, Bright, JJ. COX-2 Inhibitors Modulate IL-12 Signaling Through JAK-STAT Pathway Leading to Th1 Response in Experimental Allergic Encephalomyelitis. J Clin Immunol, 26(1), 73-85, 2006

Otteneder, MB, Knutson, CG, Daniels, JS, Hashim, M, Crews, BC, Remmel, RP, Wang, H, Rizzo, C, Marnett, LJ. In vivo oxidative metabolism of a major peroxidation-derived DNA adduct, M1dG. Proc Natl Acad Sci U S A, 103(17), 6665-9, 2006 PMCID:1458938

Rouzer, CA, Ivanova, PT, Byrne, MO, Milne, SB, Marnett, LJ, Brown, HA. Lipid profiling reveals arachidonate deficiency in RAW264.7 cells: Structural and functional implications. Biochemistry, 45(49), 14795-808, 2006 PMCID:2443946

Rouzer, CA, Tranguch, S, Wang, H, Zhang, H, Dey, SK, Marnett, LJ. Zymosan-induced glycerylprostaglandin and prostaglandin synthesis in resident peritoneal macrophages: roles of cyclo-oxygenase-1 and -2. Biochem J, 399(1), 91-9, 2006 PMCID:1570173

Wang, H, Kozekov, ID, Kozekova, A, Tamura, PJ, Marnett, LJ, Harris, TM, Rizzo, CJ. Site-specific synthesis of oligonucleotides containing malondialdehyde adducts of deoxyguanosine and deoxyadenosine via a postsynthetic modification strategy. Chem Res Toxicol, 19(11), 1467-74, 2006 PMCID:2441645

Wang, H, Xie, H, Guo, Y, Zhang, H, Takahashi, T, Kingsley, PJ, Marnett, LJ, Das, SK, Cravatt, BF, Dey, SK. Fatty acid amide hydrolase deficiency limits early pregnancy events. J Clin Invest, 116(8), 2122-31, 2006 PMCID:1523389

West, JD, Marnett, LJ. Endogenous reactive intermediates as modulators of cell signaling and cell death. Chem Res Toxicol, 19(2), 173-94, 2006

Burleigh, ME, Babaev, VR, Patel, MB, Crews, BC, Remmel, RP, Morrow, JD, Oates, JA, Marnett, LJ, Fazio, S, Linton, MF. Inhibition of cyclooxygenase with indomethacin phenethylamide reduces atherosclerosis in apoE-null mice. Biochem Pharmacol, 70(3), 334-42, 2005

Guo, Y, Wang, H, Okamoto, Y, Ueda, N, Kingsley, PJ, Marnett, LJ, Schmid, HH, Das, SK, Dey, SK. N-acylphosphatidylethanolamine-hydrolyzing phospholipase D is an important determinant of uterine anandamide levels during implantation. J Biol Chem, 280(25), 23429-32, 2005

Hata, AN, Lybrand, TP, Marnett, LJ, Breyer, RM. Structural determinants of arylacetic acid nonsteroidal anti-inflammatory drugs necessary for binding and activation of the prostaglandin D2 receptor CRTH2. Mol Pharmacol, 67(3), 640-7, 2005

Kalgutkar, AS, Crews, BC, Saleh, S, Prudhomme, D, Marnett, LJ. Indolyl esters and amides related to indomethacin are selective COX-2 inhibitors. Bioorg Med Chem, 13(24), 6810-22, 2005

Kingsley, PJ, Rouzer, CA, Saleh, S, Marnett, LJ. Simultaneous analysis of prostaglandin glyceryl esters and prostaglandins by electrospray tandem mass spectrometry. Anal Biochem, 343(2), 203-11, 2005

Moth, CW, Prusakiewicz, JJ, Marnett, LJ, Lybrand, TP. Stereoselective binding of indomethacin ethanolamide derivatives to cyclooxygenase-1. J Med Chem, 48(10), 3613-20, 2005

Rouzer, CA, Jacobs, AT, Nirodi, CS, Kingsley, PJ, Morrow, JD, Marnett, LJ. RAW264.7 cells lack prostaglandin-dependent autoregulation of tumor necrosis factor-alpha secretion. J Lipid Res, 46(5), 1027-37, 2005

Rouzer, CA, Marnett, LJ. Structural and functional differences between cyclooxygenases: fatty acid oxygenases with a critical role in cell signaling. Biochem Biophys Res Commun, 338(1), 34-44, 2005

Rouzer, CA, Marnett, LJ. Glycerylprostaglandin synthesis by resident peritoneal macrophages in response to a zymosan stimulus. J Biol Chem, 280(29), 26690-700, 2005

VanderVeen, LA, Druckova, A, Riggins, JN, Sorrells, JL, Guengerich, FP, Marnett, LJ. Differential DNA recognition and cleavage by EcoRI dependent on the dynamic equilibrium between the two forms of the malondialdehyde-deoxyguanosine adduct. Biochemistry, 44(13), 5024-33, 2005

V??lez-Cruz, R, Riggins, JN, Daniels, JS, Cai, H, Guengerich, FP, Marnett, LJ, Osheroff, N. Exocyclic DNA lesions stimulate DNA cleavage mediated by human topoisomerase II alpha in vitro and in cultured cells. Biochemistry, 44(10), 3972-81, 2005

West, JD, Ji, C, Marnett, LJ. Modulation of DNA fragmentation factor 40 nuclease activity by poly(ADP-ribose) polymerase-1. J Biol Chem, 280(15), 15141-7, 2005

West, JD, Marnett, LJ. Alterations in gene expression induced by the lipid peroxidation product, 4-hydroxy-2-nonenal. Chem Res Toxicol, 18(11), 1642-53, 2005

Cline, Susan D, Riggins, James N, Tornaletti, Silvia, Marnett, Lawrence J, Hanawalt, Philip C. Malondialdehyde adducts in DNA arrest transcription by T7 RNA polymerase and mammalian RNA polymerase II. Proc Natl Acad Sci U S A, 101(19), 7275-80, 2004 PMCID:409909

Hashim, MF, Riggins, JN, Schnetz-Boutaud, N, Voehler, M, Stone, MP, Marnett, LJ. In vitro bypass of malondialdehyde-deoxyguanosine adducts: differential base selection during extension by the Klenow fragment of DNA polymerase I is the critical determinant of replication outcome. Biochemistry, 43(37), 11828-35, 2004

J.J. Prusakiewicz, A.S. Felts, B.S. Mackenzie, and L.J. Marnett. . Molecular Basis of the Time-Dependent Inhibition of Cyclooxygenases by Indomethacin. Biochemistry, 43, 15439-15445, 2004

K.R. Kozak, J.J. Prusakiewicz, S.W. Rowlinson, D.R. Prudhomme, and L.J. Marnett.. Amino Acid Determinants in Cyclooxygenase-2 Oxygenation of the Endocannabinoid Anandamide. Biochemistry , 42, 9041-9049, 2004

Kozak, Kevin R, Prusakiewicz, Jeffery J, Marnett, Lawrence J. Oxidative metabolism of endocannabinoids by COX-2. Curr Pharm Des, 10(6), 659-67, 2004

Nirodi, Chaitanya S, Crews, Brenda C, Kozak, Kevin R, Morrow, Jason D, Marnett, Lawrence J. The glyceryl ester of prostaglandin E2 mobilizes calcium and activates signal transduction in RAW264.7 cells. Proc Natl Acad Sci U S A, 101(7), 1840-5, 2004 PMCID:357014

Prusakiewicz, JJ, Felts, AS, Mackenzie, BS, Marnett, LJ. Molecular basis of the time-dependent inhibition of cyclooxygenases by indomethacin. Biochemistry, 43(49), 15439-45, 2004

R.P. Remmel, B.C. Crews, K.R. Kozak, A.S. Kalgutkar, and L.J. Marnett. . Studies on the Metabolism of the Novel, Selective Cyclooxygenase-2 Inhibitor, Indomethacin Phenethylamide in Rat, Mouse, and Human Liver Microsomes: Identification of Active Metabolites. Drug Metab. Dispos., 32, 113-122, 2004

Remmel, RP, Crews, BC, Kozak, KR, Kalgutkar, AS, Marnett, LJ, Algutkar, AS. Studies on the metabolism of the novel, selective cyclooxygenase-2 inhibitor indomethacin phenethylamide in rat, mouse, and human liver microsomes: identification of active metabolites. Drug Metab Dispos, 32(1), 113-22, 2004

Riggins, JN, Pratt, DA, Voehler, M, Daniels, JS, Marnett, LJ. Kinetics and mechanism of the general-acid-catalyzed ring-closure of the malondialdehyde-DNA adduct, N2-(3-oxo-1-propenyl)deoxyguanosine (N2OPdG-), to 3-(2''-Deoxy-beta-D-erythro-pentofuranosyl)pyrimido[1,2-alpha]purin- 10(3H)-one (M1dG). J Am Chem Soc, 126(34), 10571-81, 2004

Riggins, James, Daniels, J., Rouzer, Carol, Marnett, Lawrence. Kinetic and Thermodynamic Analysis of the Hydrolytic Ring-Opening of the Malondialdehyde-Deoxyguanosine Adduct, 3-(2''-Deoxy-beta-d-erythro-pentofuranosyl)- pyrimido[1,2-alpha]purin-10(3H)-one. J Am Chem Soc, 126(26), 8237-8243, 2004

Rouzer, Carol A., Kingsley, Phillip J., Wang, Haibin, Zhang, Hao, Morrow, Jason D., Dey, Sudhansu K., Marnett, Lawrence J.. Cyclooxygenase-1-dependent prostaglandin synthesis modulates tumor necrosis factor alpha secretion in lipopolysachharide-challenged murine resident peritoneal macrophages. J Biol Chem, 2004

Wang, H, Guo, Y, Wang, D, Kingsley, PJ, Marnett, LJ, Das, SK, DuBois, RN, Dey, SK. Aberrant cannabinoid signaling impairs oviductal transport of embryos. Nat Med, 10(10), 1074-80, 2004

Wang, Hao, Marnett, Lawrence J, Harris, Thomas M, Rizzo, Carmelo J. A novel synthesis of malondialdehyde adducts of deoxyguanosine, deoxyadenosine, and deoxycytidine. Chem Res Toxicol, 17(2), 144-9, 2004

West, James D, Ji, Chuan, Duncan, Stephen T, Amarnath, Venkataraman, Schneider, Claus, Rizzo, Carmelo J, Brash, Alan R, Marnett, Lawrence J. Induction of apoptosis in colorectal carcinoma cells treated with 4-hydroxy-2-nonenal and structurally related aldehydic products of lipid peroxidation. Chem Res Toxicol, 17(4), 453-62, 2004

Aronoff, David M, Boutaud, Olivier, Marnett, Lawrence J, Oates, John A. Inhibition of prostaglandin H2 synthases by salicylate is dependent on the oxidative state of the enzymes. J Pharmacol Exp Ther, 304(2), 589-95, 2003

Kingsley, Philip J, Marnett, Lawrence J. Analysis of endocannabinoids by Ag(+) coordination tandem mass spectrometry. Anal Biochem, 314(1), 8-15, 2003

Kozak, Kevin R, Prusakiewicz, Jeffery J, Rowlinson, Scott W, Prudhomme, Daniel R, Marnett, Lawrence J. Amino Acid determinants in cyclooxygenase-2 oxygenation of the endocannabinoid anandamide. Biochemistry, 42(30), 9041-9, 2003

Marnett, Lawrence J, Riggins, James N, West, James D. Endogenous generation of reactive oxidants and electrophiles and their reactions with DNA and protein. J Clin Invest, 111(5), 583-93, 2003 PMCID:151910

Niedernhofer, Laura J., Daniels, John S., Rouzer, Carol A., Greene, Rachel E., Marnett, Lawrence J. Malondialdehyde, a product of lipid peroxidation, is mutagenic in human cellsi. J Biol Chem, 278, 31426-31433, 2003

Otteneder, M, Scott Daniels, J, Voehler, M, Marnett, LJ. Development of a method for determination of the malondialdehyde-deoxyguanosine adduct in urine using liquid chromatography-tandem mass spectrometry. Anal Biochem, 315(2), 147-51, 2003

Phillip Hochgesang, G, Nemeth-Cawley, Jennifer F, Rowlinson, Scott W, Caprioli, Richard M, Marnett, Lawrence J. Functional analysis of the molecular determinants of cyclooxygenase-2 acetylation by 2-acetoxyphenylhept-2-ynyl sulfide. Arch Biochem Biophys, 409(1), 127-33, 2003

Rouzer, Carol A, Marnett, Lawrence J. Mechanism of free radical oxygenation of polyunsaturated fatty acids by cyclooxygenases. Chem Rev, 103(6), 2239-304, 2003

Rowlinson, Scott W, Kiefer, James R, Prusakiewicz, Jeffery J, Pawlitz, Jennifer L, Kozak, Kevin R, Kalgutkar, Amit S, Stallings, William C, Kurumbail, Ravi G, Marnett, Lawrence J. A novel mechanism of cyclooxygenase-2 inhibition involving interactions with Ser-530 and Tyr-385. J Biol Chem, 278(46), 45763-9, 2003

VanderVeen, Laurie A, Hashim, Muhammed F, Shyr, Yu, Marnett, Lawrence J. Induction of frameshift and base pair substitution mutations by the major DNA adduct of the endogenous carcinogen malondialdehyde. Proc Natl Acad Sci U S A, 100(24), 14247-52, 2003 PMCID:283577

Yu, Z, Schneider, C, Boeglin, WE, Marnett, LJ, Brash, AR. The lipoxygenase gene ALOXE3 implicated in skin differentiation encodes a hydroperoxide isomerase. Proc Natl Acad Sci U S A, 100(16), 9162-7, 2003 PMCID:170889

A.S. Kalgutkar, S.W. Rowlinson, B.C. Crews, and L.J. Marnett. . Amide Derivatives of Meclofenamic Acid as Selective Cyclooxygenase-2 Inhibitors. Bioorg. Med. Chem. Lett, 12, 521-524, 2002

Boutaud, Olivier, Aronoff, David M, Richardson, Jacob H, Marnett, Lawrence J, Oates, John A. Determinants of the cellular specificity of acetaminophen as an inhibitor of prostaglandin H(2) synthases. Proc Natl Acad Sci U S A, 99(10), 7130-5, 2002 PMCID:124540

Burleigh, ME, Babaev, VR, Oates, JA, Harris, RC, Gautam, S, Riendeau, D, Marnett, LJ, Morrow, JD, Fazio, S, Linton, MF. Cyclooxygenase-2 promotes early atherosclerotic lesion formation in LDL receptor-deficient mice. Circulation, 105(15), 1816-23, 2002

C.A. Rouzer, K. Ghebreselasi, and L.J. Marnett. Chemical Stability of 2-Arachidonylglycerol under Biological Conditions. Chem. Phys. Lipids , 119, 69-82, 2002

J.S. Moody and L.J. Marnett. Kinetics of Inhibition of Leukocyte 12-Lipoxygenase by the Isoform-Specific Inhibitor 4-(2-Oxapentadeca-4-yne)-phenylpropanoic Acid. Biochemistry, 41, 10297-10303, 2002

Kalgutkar, AS, Rowlinson, SW, Crews, BC, Marnett, LJ. Amide derivatives of meclofenamic acid as selective cyclooxygenase-2 inhibitors. Bioorg Med Chem Lett, 12(4), 521-4, 2002

Kozak, KR, Marnett, LJ. Oxidative metabolism of endocannabinoids. Prostaglandins Leukot Essent Fatty Acids, 66(2-3), 211-20, 2002

Kozak, Kevin R, Crews, Brenda C, Morrow, Jason D, Wang, Lee-Ho, Ma, Y Henry, Weinander, Rolf, Jakobsson, Per-Johan, Marnett, Lawrence J. Metabolism of the endocannabinoids, 2-arachidonylglycerol and anandamide, into prostaglandin, thromboxane, and prostacyclin glycerol esters and ethanolamides. J Biol Chem, 277(47), 44877-85, 2002

Kozak, Kevin R, Gupta, Rajnish A, Moody, John S, Ji, Chuan, Boeglin, William E, DuBois, Raymond N, Brash, Alan R, Marnett, Lawrence J. 15-Lipoxygenase metabolism of 2-arachidonylglycerol. Generation of a peroxisome proliferator-activated receptor alpha agonist. J Biol Chem, 277(26), 23278-86, 2002

Kozak, Kevin R, Prusakiewicz, Jeffery J, Rowlinson, Scott W, Marnett, Lawrence J. Enantiospecific, selective cyclooxygenase-2 inhibitors. Bioorg Med Chem Lett, 12(9), 1315-8, 2002

L.J. Marnett. Oxy Radicals, Lipid Peroxidation and DNA Damage. Toxicology, 181-182, 219-222, 2002

Marnett, LJ. Recent developments in cyclooxygenase inhibition. Prostaglandins Other Lipid Mediat, 68-69, 153-64, 2002

Marnett, LJ. Oxy radicals, lipid peroxidation and DNA damage. Toxicology, 181-182, 219-22, 2002

Marnett, Lawrence J, DuBois, Raymond N. COX-2: a target for colon cancer prevention. Annu Rev Pharmacol Toxicol, 42, 55-80, 2002

Moody, JS, Marnett, LJ. Kinetics of inhibition of leukocyte 12-lipoxygenase by the isoform-specific inhibitor 4-(2-oxapentadeca-4-yne)phenylpropanoic acid. Biochemistry, 41(32), 10297-303, 2002

Otteneder, M, Plastaras, JP, Marnett, LJ. Reaction of malondialdehyde-DNA adducts with hydrazines-development of a facile assay for quantification of malondialdehyde equivalents in DNA. Chem Res Toxicol, 15(3), 312-8, 2002

Plastaras, JP, Dedon, PC, Marnett, LJ. Effects of DNA structure on oxopropenylation by the endogenous mutagens malondialdehyde and base propenal. Biochemistry, 41(15), 5033-42, 2002

Prusakiewicz, Jeffery J, Kingsley, Philip J, Kozak, Kevin R, Marnett, Lawrence J. Selective oxygenation of N-arachidonylglycine by cyclooxygenase-2. Biochem Biophys Res Commun, 296(3), 612-7, 2002

Rouzer, CA, Ghebreselasie, K, Marnett, LJ. Chemical stability of 2-arachidonylglycerol under biological conditions. Chem Phys Lipids, 119(1-2), 69-82, 2002

S.L. Timofeevski, J.J. Prusakiewicz, C.A. Rouzer, and L.J. Marnett.. Isoform-Selective Interaction of Cyclooxygenase-2 with Indomethacin Amides Studied by Real-Time Fluorescence, Inhibition Kinetics, and Site-Directed Mutagenesis. Biochemistry, 41, 9654-9662, 2002

Schneider, C, Boeglin, WE, Prusakiewicz, JJ, Rowlinson, SW, Marnett, LJ, Samel, N, Brash, AR. Control of prostaglandin stereochemistry at the 15-carbon by cyclooxygenases-1 and -2. A critical role for serine 530 and valine 349. J Biol Chem, 277(1), 478-85, 2002

Sekhar, KR, Spitz, DR, Harris, S, Nguyen, TT, Meredith, MJ, Holt, JT, Gius, D, Marnett, LJ, Summar, ML, Freeman, ML, Guis, D. Redox-sensitive interaction between KIAA0132 and Nrf2 mediates indomethacin-induced expression of gamma-glutamylcysteine synthetase. Free Radic Biol Med, 32(7), 650-62, 2002

Timofeevski, SL, Prusakiewicz, JJ, Rouzer, CA, Marnett, LJ. Isoform-selective interaction of cyclooxygenase-2 with indomethacin amides studied by real-time fluorescence, inhibition kinetics, and site-directed mutagenesis. Biochemistry, 41(30), 9654-62, 2002

Weisenseel, JP, Reddy, GR, Marnett, LJ, Stone, MP. Structure of the 1,N(2)-propanodeoxyguanosine adduct in a three-base DNA hairpin loop derived from a palindrome in the Salmonella typhimurium hisD3052 gene. Chem Res Toxicol, 15(2), 140-52, 2002

Weisenseel, JP, Reddy, GR, Marnett, LJ, Stone, MP. Structure of an oligodeoxynucleotide containing a 1,N(2)-propanodeoxyguanosine adduct positioned in a palindrome derived from the Salmonella typhimurium hisD3052 gene: Hoogsteen pairing at pH 5.2. Chem Res Toxicol, 15(2), 127-39, 2002

Boutaud, O, Brame, CJ, Chaurand, P, Li, J, Rowlinson, SW, Crews, BC, Ji, C, Marnett, LJ, Caprioli, RM, Roberts, LJ, Oates, JA. Characterization of the lysyl adducts of prostaglandin H-synthases that are derived from oxygenation of arachidonic acid. Biochemistry, 40(23), 6948-55, 2001

Boutaud, O, Li, J, Chaurand, P, Brame, CJ, Marnett, LJ, Roberts, LJ, Oates, JA. Oxygenation of arachidonic acid by cyclooxygenases generates reactive intermediates that form adducts with proteins. Adv Exp Med Biol, 500, 133-7, 2001

Fink, SP, Marnett, LJ. The relative contribution of adduct blockage and DNA repair on template utilization during replication of 1,N2-propanodeoxyguanosine and pyrimido. Mutat Res, 485(3), 209-18, 2001

Ji, C, Amarnath, V, Pietenpol, JA, Marnett, LJ. 4-hydroxynonenal induces apoptosis via caspase-3 activation and cytochrome c release. Chem Res Toxicol, 14(8), 1090-6, 2001

Ji, C, Kozak, KR, Marnett, LJ. IkappaB kinase, a molecular target for inhibition by 4-hydroxy-2-nonenal. J Biol Chem, 276(21), 18223-8, 2001

K.R. Kozak, J.J. Prusakiewicz, S.W. Rowlinson, C. Schneider, and L.J. Marnett.. Amino Acid Determinants in Cyclooxygenase-2 Oxygenation of the Endocannabinoid 2-Arachidonyl-glycerol. J. Biol. Chem, 276, 30072-30077, 2001

Kozak, KR, Crews, BC, Ray, JL, Tai, HH, Morrow, JD, Marnett, LJ. Metabolism of prostaglandin glycerol esters and prostaglandin ethanolamides in vitro and in vivo. J Biol Chem, 276(40), 36993-8, 2001

Kozak, KR, Prusakiewicz, JJ, Rowlinson, SW, Schneider, C, Marnett, LJ. Amino acid determinants in cyclooxygenase-2 oxygenation of the endocannabinoid 2-arachidonylglycerol. J Biol Chem, 276(32), 30072-7, 2001

Kurumbail, R G, Kiefer, J R, Marnett, L J. Cyclooxygenase enzymes: catalysis and inhibition. Curr Opin Struct Biol, 11(6), 752-60, 2001

Marnett, LJ, Plastaras, JP. Endogenous DNA damage and mutation. Trends Genet, 17(4), 214-21, 2001

Moody, JS, Kozak, KR, Ji, C, Marnett, LJ. Selective oxygenation of the endocannabinoid 2-arachidonylglycerol by leukocyte-type 12-lipoxygenase. Biochemistry, 40(4), 861-6, 2001

Nemeth, JF, Hochgesang, GP, Marnett, LJ, Caprioli, RM, Hochensang, GP. Characterization of the glycosylation sites in cyclooxygenase-2 using mass spectrometry. Biochemistry, 40(10), 3109-16, 2001

Riggins, JN, Marnett, LJ. Mutagenicity of the malondialdehyde oligomerization products 2-(3''-oxo-1''-propenyl)-malondialdehyde and 2,4-dihydroxymethylene-3-(2,2-dimethoxyethyl)glutaraldehyde in Salmonella. Mutat Res, 497(1-2), 153-7, 2001

Schnetz-Boutaud, NC, Chapeau, MC, Marnett, LJ. Enzymatic synthesis of M(1)G-deoxyribose. Curr Protoc Nucleic Acid Chem, Chapter 1, Unit1.2, 2001

Schnetz-Boutaud, NC, Saleh, S, Marnett, LJ, Stone, MP. The exocyclic 1,N2-deoxyguanosine pyrimidopurinone M1G is a chemically stable DNA adduct when placed opposite a two-base deletion in the (CpG)3 frameshift hotspot of the Salmonella typhimurium hisD3052 gene. Biochemistry, 40(51), 15638-49, 2001

Schnetz-Boutaud, NC, Saleh, S, Marnett, LJ, Stone, MP. Structure of the malondialdehyde deoxyguanosine adduct M1G when placed opposite a two-base deletion in the (CpG)3 frameshift hotspot of the Salmonella typhimurium hisD3052 gene. Adv Exp Med Biol, 500, 513-6, 2001

VanderVeen, LA, Hashim, MF, Nechev, LV, Harris, TM, Harris, CM, Marnett, LJ. Evaluation of the mutagenic potential of the principal DNA adduct of acrolein. J Biol Chem, 276(12), 9066-70, 2001

Goodwin, DC, Rowlinson, SW, Marnett, LJ. Substitution of tyrosine for the proximal histidine ligand to the heme of prostaglandin endoperoxide synthase 2: implications for the mechanism of cyclooxygenase activation and catalysis. Biochemistry, 39(18), 5422-32, 2000

Kalgutkar, AS, Crews, BC, Rowlinson, SW, Marnett, AB, Kozak, KR, Remmel, RP, Marnett, LJ. Biochemically based design of cyclooxygenase-2 (COX-2) inhibitors: facile conversion of nonsteroidal antiinflammatory drugs to potent and highly selective COX-2 inhibitors. Proc Natl Acad Sci U S A, 97(2), 925-30, 2000 PMCID:15432

Kalgutkar, AS, Marnett, AB, Crews, BC, Remmel, RP, Marnett, LJ. Ester and amide derivatives of the nonsteroidal antiinflammatory drug, indomethacin, as selective cyclooxygenase-2 inhibitors. J Med Chem, 43(15), 2860-70, 2000

Kozak, KR, Rowlinson, SW, Marnett, LJ. Oxygenation of the endocannabinoid, 2-arachidonylglycerol, to glyceryl prostaglandins by cyclooxygenase-2. J Biol Chem, 275(43), 33744-9, 2000

Lanzo, CA, Sutin, J, Rowlinson, S, Talley, J, Marnett, LJ. Fluorescence quenching analysis of the association and dissociation of a diarylheterocycle to cyclooxygenase-1 and cyclooxygenase-2: dynamic basis of cyclooxygenase-2 selectivity. Biochemistry, 39(20), 6228-34, 2000

Marnett, LJ. Structure, function and inhibition of cyclo-oxygenases. Ernst Schering Res Found Workshop(31), 65-83, 2000

Marnett, LJ. Cyclooxygenase mechanisms. Curr Opin Chem Biol, 4(5), 545-52, 2000

Marnett, LJ. Oxyradicals and DNA damage. Carcinogenesis, 21(3), 361-70, 2000

Marnett, LJ, Wright, TL, Crews, BC, Tannenbaum, SR, Morrow, JD. Regulation of prostaglandin biosynthesis by nitric oxide is revealed by targeted deletion of inducible nitric-oxide synthase. J Biol Chem, 275(18), 13427-30, 2000

Plastaras, JP, Guengerich, FP, Nebert, DW, Marnett, LJ. Xenobiotic-metabolizing cytochromes P450 convert prostaglandin endoperoxide to hydroxyheptadecatrienoic acid and the mutagen, malondialdehyde. J Biol Chem, 275(16), 11784-90, 2000

Plastaras, JP, Riggins, JN, Otteneder, M, Marnett, LJ. Reactivity and mutagenicity of endogenous DNA oxopropenylating agents: base propenals, malondialdehyde, and N(epsilon)-oxopropenyllysine. Chem Res Toxicol, 13(12), 1235-42, 2000

Rowlinson, SW, Crews, BC, Goodwin, DC, Schneider, C, Gierse, JK, Marnett, LJ. Spatial requirements for 15-(R)-hydroxy-5Z,8Z,11Z, 13E-eicosatetraenoic acid synthesis within the cyclooxygenase active site of murine COX-2. Why acetylated COX-1 does not synthesize 15-(R)-hete. J Biol Chem, 275(9), 6586-91, 2000

Schnetz-Boutaud, N, Daniels, JS, Hashim, MF, Scholl, P, Burrus, T, Marnett, LJ. Pyrimido[1,2-alpha]purin-10(3H)-one: a reactive electrophile in the genome. Chem Res Toxicol, 13(10), 967-70, 2000

Schnetz-Boutaud, NC, Mao, H, Stone, MP, Marnett, LJ. Synthesis of oligonucleotides containing the alkali-labile pyrimidopurinone adduct, M(1)G. Chem Res Toxicol, 13(2), 90-5, 2000

Daniel, TO, Liu, H, Morrow, JD, Crews, BC, Marnett, LJ. Thromboxane A2 is a mediator of cyclooxygenase-2-dependent endothelial migration and angiogenesis. Cancer Res, 59(18), 4574-7, 1999

Goodwin, DC, Landino, LM, Marnett, LJ. Effects of nitric oxide and nitric oxide-derived species on prostaglandin endoperoxide synthase and prostaglandin biosynthesis. FASEB J, 13(10), 1121-36, 1999

Goodwin, DC, Landino, LM, Marnett, LJ. Reactions of prostaglandin endoperoxide synthase with nitric oxide and peroxynitrite. Drug Metab Rev, 31(1), 273-94, 1999

Johnson, KA, Mierzwa, ML, Fink, SP, Marnett, LJ. MutS recognition of exocyclic DNA adducts that are endogenous products of lipid oxidation. J Biol Chem, 274(38), 27112-8, 1999

Kalgutkar, AS, Crews, BC, Rowlinson, SW, Garner, C, Marnett, LJ. Discovery of a new class of selective cyclooxygenase-2 (COX-2) inhibitor that covalently modifies the isozyme. Adv Exp Med Biol, 469, 139-43, 1999

Mao, H, Reddy, GR, Marnett, LJ, Stone, MP. Solution structure of an oligodeoxynucleotide containing the malondialdehyde deoxyguanosine adduct N2-(3-oxo-1-propenyl)-dG (ring-opened M1G) positioned in a (CpG)3 frameshift hotspot of the Salmonella typhimurium hisD3052 gene. Biochemistry, 38(41), 13491-501, 1999

Mao, H, Schnetz-Boutaud, NC, Weisenseel, JP, Marnett, LJ, Stone, MP. Duplex DNA catalyzes the chemical rearrangement of a malondialdehyde deoxyguanosine adduct. Proc Natl Acad Sci U S A, 96(12), 6615-20, 1999 PMCID:21963

Marnett, LJ. Chemistry and biology of DNA damage by malondialdehyde. IARC Sci Publ(150), 17-27, 1999

Marnett, LJ. Lipid peroxidation-DNA damage by malondialdehyde. Mutat Res, 424(1-2), 83-95, 1999

Marnett, LJ, Kalgutkar, AS. Cyclooxygenase 2 inhibitors: discovery, selectivity and the future. Trends Pharmacol Sci, 20(11), 465-9, 1999

Marnett, LJ, Rowlinson, SW, Goodwin, DC, Kalgutkar, AS, Lanzo, CA. Arachidonic acid oxygenation by COX-1 and COX-2. Mechanisms of catalysis and inhibition. J Biol Chem, 274(33), 22903-6, 1999

Montine, TJ, Sidell, KR, Crews, BC, Markesbery, WR, Marnett, LJ, Roberts, LJ, Morrow, JD. Elevated CSF prostaglandin E2 levels in patients with probable AD. Neurology, 53(7), 1495-8, 1999

Richards, KM, Moody, JS, Marnett, LJ. Mechanism of inhibition of porcine leukocyte 12-lipoxygenase by the isoform-specific inhibitor 4-(2-oxapentadeca-4-yne)phenylpropanoic acid. Biochemistry, 38(50), 16529-38, 1999

Rowlinson, SW, Crews, BC, Lanzo, CA, Marnett, LJ. The binding of arachidonic acid in the cyclooxygenase active site of mouse prostaglandin endoperoxide synthase-2 (COX-2). A putative L-shaped binding conformation utilizing the top channel region. J Biol Chem, 274(33), 23305-10, 1999

Goodwin, DC, Gunther, MR, Hsi, LC, Crews, BC, Eling, TE, Mason, RP, Marnett, LJ. Nitric oxide trapping of tyrosyl radicals generated during prostaglandin endoperoxide synthase turnover. Detection of the radical derivative of tyrosine 385. J Biol Chem, 273(15), 8903-9, 1998

Ji, C, Rouzer, CA, Marnett, LJ, Pietenpol, JA. Induction of cell cycle arrest by the endogenous product of lipid peroxidation, malondialdehyde. Carcinogenesis, 19(7), 1275-83, 1998

Kalgutkar, AS, Crews, BC, Rowlinson, SW, Garner, C, Seibert, K, Marnett, LJ. Aspirin-like molecules that covalently inactivate cyclooxygenase-2. Science, 280(5367), 1268-70, 1998

Kalgutkar, AS, Kozak, KR, Crews, BC, Hochgesang, GP, Marnett, LJ. Covalent modification of cyclooxygenase-2 (COX-2) by 2-acetoxyphenyl alkyl sulfides, a new class of selective COX-2 inactivators. J Med Chem, 41(24), 4800-18, 1998

Langou?

Lanzo, CA, Beechem, JM, Talley, J, Marnett, LJ. Investigation of the binding of isoform-selective inhibitors to prostaglandin endoperoxide synthases using fluorescence spectroscopy. Biochemistry, 37(1), 217-26, 1998

Marnett, LJ, Kalgutkar, AS. Design of selective inhibitors of cyclooxygenase-2 as nonulcerogenic anti-inflammatory agents. Curr Opin Chem Biol, 2(4), 482-90, 1998

Fink, SP, Reddy, GR, Marnett, LJ. Mutagenicity in Escherichia coli of the major DNA adduct derived from the endogenous mutagen malondialdehyde. Proc Natl Acad Sci U S A, 94(16), 8652-7, 1997 PMCID:23062

Hashim, MF, Schnetz-Boutaud, N, Marnett, LJ. Replication of template-primers containing propanodeoxyguanosine by DNA polymerase beta. Induction of base pair substitution and frameshift mutations by template slippage and deoxynucleoside triphosphate stabilization. J Biol Chem, 272(32), 20205-12, 1997

Ji, C, Marnett, LJ, Pietenpol, JA. Cell cycle re-entry following chemically-induced cell cycle synchronization leads to elevated p53 and p21 protein levels. Oncogene, 15(22), 2749-53, 1997

Johnson, KA, Fink, SP, Marnett, LJ. Repair of propanodeoxyguanosine by nucleotide excision repair in vivo and in vitro. J Biol Chem, 272(17), 11434-8, 1997

Kalgutkar, AS, Crews, BC, Marnett, LJ. Inactivation of prostaglandin endoperoxide synthase (PGHS) by N-(substituted)maleimides. Adv Exp Med Biol, 407, 79-85, 1997

Landino, LM, Crews, BC, Gierse, JK, Hauser, SD, Marnett, LJ. Mutational analysis of the role of the distal histidine and glutamine residues of prostaglandin-endoperoxide synthase-2 in peroxidase catalysis, hydroperoxide reduction, and cyclooxygenase activation. J Biol Chem, 272(34), 21565-74, 1997

Niedernhofer, LJ, Riley, M, Schnetz-Boutaud, N, Sanduwaran, G, Chaudhary, AK, Reddy, GR, Marnett, LJ. Temperature-dependent formation of a conjugate between tris(hydroxymethyl)aminomethane buffer and the malondialdehyde-DNA adduct pyrimidopurinone. Chem Res Toxicol, 10(5), 556-61, 1997

Richards, KM, Marnett, LJ. Leukocyte 12-lipoxygenase: expression, purification, and investigation of the role of methionine residues in turnover-dependent inactivation and 5,8,11,14-eicosatetraynoic acid inhibition. Biochemistry, 36(22), 6692-9, 1997

Rouzer, CA, Chaudhary, AK, Nokubo, M, Ferguson, DM, Reddy, GR, Blair, IA, Marnett, LJ. Analysis of the malondialdehyde-2''-deoxyguanosine adduct pyrimidopurinone in human leukocyte DNA by gas chromatography/electron capture/negative chemical ionization/mass spectrometry. Chem Res Toxicol, 10(2), 181-8, 1997

Smith, CJ, Morrow, JD, Roberts, LJ, Marnett, LJ. Induction of prostaglandin endoperoxide synthase-1 (COX-1) in a human promonocytic cell line by treatment with the differentiating agent TPA. Adv Exp Med Biol, 400A, 99-106, 1997

Chaudhary, AK, Reddy, GR, Blair, IA, Marnett, LJ. Characterization of an N6-oxopropenyl-2''-deoxyadenosine adduct in malondialdehyde-modified DNA using liquid chromatography/electrospray ionization tandem mass spectrometry. Carcinogenesis, 17(5), 1167-70, 1996

Fink, SP, Reddy, GR, Marnett, LJ. Relative contribution of cytosine deamination and error-prone replication to the induction of propanodeoxyguanosine-->deoxyadenosine mutations in Escherichia coli. Chem Res Toxicol, 9(1), 277-83, 1996

Hashim, MF, Marnett, LJ. Sequence-dependent induction of base pair substitutions and frameshifts by propanodeoxyguanosine during in vitro DNA replication. J Biol Chem, 271(15), 9160-5, 1996

Kalgutkar, AS, Crews, BC, Marnett, LJ. Design, synthesis, and biochemical evaluation of N-substituted maleimides as inhibitors of prostaglandin endoperoxide synthases. J Med Chem, 39(8), 1692-703, 1996

Kalgutkar, AS, Crews, BC, Marnett, LJ. Kinetics of the interaction of nonsteroidal antiinflammatory drugs with prostaglandin endoperoxide synthase-1 studied by limited proteolysis. Biochemistry, 35(28), 9076-82, 1996

Landino, LM, Crews, BC, Timmons, MD, Morrow, JD, Marnett, LJ. Peroxynitrite, the coupling product of nitric oxide and superoxide, activates prostaglandin biosynthesis. Proc Natl Acad Sci U S A, 93(26), 15069-74, 1996 PMCID:26357

Landino, LM, Marnett, LJ. Mechanism of hydroperoxide reduction by mangano-prostaglandin endoperoxide synthase. Biochemistry, 35(8), 2637-43, 1996

Reddy, GR, Marnett, LJ. Mechanism of reaction of beta-(aryloxy)acroleins with nucleosides. Chem Res Toxicol, 9(1), 12-5, 1996

Smith, CJ, Marnett, LJ. Effects of cysteine-to-serine mutations on structural and functional properties of prostaglandin endoperoxide synthase. Arch Biochem Biophys, 335(2), 342-50, 1996

Benamira, M, Johnson, K, Chaudhary, A, Bruner, K, Tibbetts, C, Marnett, LJ. Induction of mutations by replication of malondialdehyde-modified M13 DNA in Escherichia coli: determination of the extent of DNA modification, genetic requirements for mutagenesis, and types of mutations induced. Carcinogenesis, 16(1), 93-9, 1995

Kingma, PS, Corbett, AH, Burcham, PC, Marnett, LJ, Osheroff, N. Abasic sites stimulate double-stranded DNA cleavage mediated by topoisomerase II. DNA lesions as endogenous topoisomerase II poisons. J Biol Chem, 270(37), 21441-4, 1995

Marnett, LJ. Aspirin and related nonsteroidal anti-inflammatory drugs as chemopreventive agents against colon cancer. Prev Med, 24(2), 103-6, 1995

Marnett, LJ, Wilcox, AL. The chemistry of lipid alkoxyl radicals and their role in metal-amplified lipid peroxidation. Biochem Soc Symp, 61, 65-72, 1995

Weisenseel, JP, Moe, JG, Reddy, GR, Marnett, LJ, Stone, MP. Structure of a duplex oligodeoxynucleotide containing propanodeoxyguanosine opposite a two-base deletion in the (CpG)3 frame shift hotspot of Salmonella typhimurium hisD3052 determined by 1H NMR and restrained molecular dynamics. Biochemistry, 34(1), 50-64, 1995

Beckman, JK, Bagheri, F, Ji, C, Blair, IA, Marnett, LJ. Phospholipid peroxidation in tumor promoter-exposed mouse skin. Carcinogenesis, 15(12), 2937-44, 1994

Burcham, PC, Marnett, LJ. Site-specific mutagenesis by a propanodeoxyguanosine adduct carried on an M13 genome. J Biol Chem, 269(46), 28844-50, 1994

Chaudhary, AK, Nokubo, M, Marnett, LJ, Blair, IA. Analysis of the malondialdehyde-2''-deoxyguanosine adduct in rat liver DNA by gas chromatography/electron capture negative chemical ionization mass spectrometry. Biol Mass Spectrom, 23(8), 457-64, 1994

Chaudhary, AK, Nokubo, M, Reddy, GR, Yeola, SN, Morrow, JD, Blair, IA, Marnett, LJ. Detection of endogenous malondialdehyde-deoxyguanosine adducts in human liver. Science, 265(5178), 1580-2, 1994

Kalgutkar, AS, Marnett, LJ. Rapid inactivation of prostaglandin endoperoxide synthases by N-(carboxyalkyl)maleimides. Biochemistry, 33(29), 8625-8, 1994

Kennedy, TA, Smith, CJ, Marnett, LJ. Investigation of the role of cysteines in catalysis by prostaglandin endoperoxide synthase. J Biol Chem, 269(44), 27357-64, 1994

Marnett, LJ. DNA adducts of alpha,beta-unsaturated aldehydes and dicarbonyl compounds. IARC Sci Publ(125), 151-63, 1994

Marnett, LJ. Generation of mutagens during arachidonic acid metabolism. Cancer Metastasis Rev, 13(3-4), 303-8, 1994

Marnett, LJ, Honn, KV. Overview of articles on eicosanoids and cancer. Cancer Metastasis Rev, 13(3-4), 237-9, 1994

Marnett, LJ, Ji, C. Modulation of oxidant formation in mouse skin in vivo by tumor-promoting phorbol esters. Cancer Res, 54(7 Suppl), 1886s-1889s, 1994

Moe, JG, Reddy, GR, Marnett, LJ, Stone, MP. 1H NMR characterization of a duplex oligodeoxynucleotide containing propanodeoxyguanosine opposite a two-base deletion in the (CpG)3 frameshift hotspot of Salmonella typhimurium hisD3052. Chem Res Toxicol, 7(3), 319-28, 1994

Reddy, RG, Yoshimoto, T, Yamamoto, S, Funk, CD, Marnett, LJ. Expression of porcine leukocyte 12-lipoxygenase in a baculovirus/insect cell system and its characterization. Arch Biochem Biophys, 312(1), 219-26, 1994

Reddy, RG, Yoshimoto, T, Yamamoto, S, Marnett, LJ. Expression, purification, and characterization of porcine leukocyte 12-lipoxygenase produced in the methylotrophic yeast, Pichia pastoris. Biochem Biophys Res Commun, 205(1), 381-8, 1994

Benamira, M, Marnett, LJ. Construction of a vector for site-specific frameshift mutagenesis containing the mutable hotspot of Salmonella typhimurium TA98 on an M13 bacteriophage. Chem Res Toxicol, 6(3), 317-27, 1993

Marnett, LJ, Burcham, PC. Endogenous DNA adducts: potential and paradox. Chem Res Toxicol, 6(6), 771-85, 1993

Singh, US, Moe, JG, Reddy, GR, Weisenseel, JP, Marnett, LJ, Stone, MP. 1H NMR of an oligodeoxynucleotide containing a propanodeoxyguanosine adduct positioned in a (CG)3 frameshift hotspot of Salmonella typhimurium hisD3052: Hoogsteen base-pairing at pH 5.8. Chem Res Toxicol, 6(6), 825-36, 1993

Smith, CJ, Morrow, JD, Roberts, LJ, Marnett, LJ. Differentiation of monocytoid THP-1 cells with phorbol ester induces expression of prostaglandin endoperoxide synthase-1 (COX-1). Biochem Biophys Res Commun, 192(2), 787-93, 1993

Wells, I, Marnett, LJ. Inactivation of prostaglandin endoperoxide synthase by acylating derivatives of indomethacin. Biochemistry, 32(10), 2710-6, 1993

Wilcox, AL, Marnett, LJ. Polyunsaturated fatty acid alkoxyl radicals exist as carbon-centered epoxyallylic radicals: a key step in hydroperoxide-amplified lipid peroxidation. Chem Res Toxicol, 6(4), 413-6, 1993

Benamira, M, Marnett, LJ. The lipid peroxidation product 4-hydroxynonenal is a potent inducer of the SOS response. Mutat Res, 293(1), 1-10, 1992

Benamira, M, Singh, U, Marnett, LJ. Site-specific frameshift mutagenesis by a propanodeoxyguanosine adduct positioned in the (CpG)4 hot-spot of Salmonella typhimurium hisD3052 carried on an M13 vector. J Biol Chem, 267(31), 22392-400, 1992

Jajoo, HK, Burcham, PC, Goda, Y, Blair, IA, Marnett, LJ. A thermospray liquid chromatography/mass spectrometry method for analysis of human urine for the major malondialdehyde-guanine adduct. Chem Res Toxicol, 5(6), 870-5, 1992

Ji, C, Marnett, LJ. Oxygen radical-dependent epoxidation of (7S,8S)-dihydroxy-7,8-dihydrobenzo[a]pyrene in mouse skin in vivo. Stimulation by phorbol esters and inhibition by antiinflammatory steroids. J Biol Chem, 267(25), 17842-8, 1992

Marnett, LJ. Aspirin and the potential role of prostaglandins in colon cancer. Cancer Res, 52(20), 5575-89, 1992

Odenwaller, R, Maddipati, KR, Marnett, LJ. Detection of a higher oxidation state of manganese-prostaglandin endoperoxide synthase. J Biol Chem, 267(20), 13863-9, 1992

Pal, I, Odenwaller, R, Marnett, LJ. 7-Oxabicycloheptylprostanoic acids: potent, time-dependent cyclooxygenase inhibitors that induce a conformational change in the prostaglandin endoperoxide synthase protein. J Med Chem, 35(12), 2340-2, 1992

Wells, I, Marnett, LJ. Acetylation of prostaglandin endoperoxide synthase by N-acetylimidazole: comparison to acetylation by aspirin. Biochemistry, 31(40), 9520-5, 1992

Chapeau, MC, Marnett, LJ. Enzymatic synthesis of purine deoxynucleoside adducts. Chem Res Toxicol, 4(6), 636-8, 1991

Goda, Y, Marnett, LJ. High-performance liquid chromatography with electrochemical detection for determination of the major malondialdehyde-guanine adduct. Chem Res Toxicol, 4(5), 520-4, 1991

Marnett, LJ. Peroxyl free radicals: biological reactive intermediates produced during lipid oxidation. Adv Exp Med Biol, 283, 65-70, 1991

Marnett, LJ, Leithauser, MT, Richards, KM, Blair, I, Honn, KV, Yamamoto, S, Yoshimoto, T. Arachidonic acid metabolism of cytosolic fractions of Lewis lung carcinoma cells. Adv Prostaglandin Thromboxane Leukot Res, 21B, 895-900, 1991

Odenwaller, R, O''Gara, CY, Marnett, LJ. Properties of trypsin-cleaved PGH synthase. Adv Prostaglandin Thromboxane Leukot Res, 21A, 81-4, 1991

Marnett, LJ. Prostaglandin synthase-mediated metabolism of carcinogens and a potential role for peroxyl radicals as reactive intermediates. Environ Health Perspect, 88, 5-12, 1990 PMCID:1568014

Marnett, LJ, Reddy, A, Pruess-Schwartz, D, Ji, C. Oxidation of polycyclic hydrocarbons by oxygen radicals. Princess Takamatsu Symp, 21, 63-73, 1990

Stone, K, Uzieblo, A, Marnett, LJ. Studies of the reaction of malondialdehyde with cytosine nucleosides. Chem Res Toxicol, 3(5), 467-72, 1990

Shimada, T, Martin, MV, Pruess-Schwartz, D, Marnett, LJ, Guengerich, FP. Roles of individual human cytochrome P-450 enzymes in the bioactivation of benzo(a)pyrene, 7,8-dihydroxy-7,8-dihydrobenzo(a)pyrene, and other dihydrodiol derivatives of polycyclic aromatic hydrocarbons. Cancer Res, 49(22), 6304-12, 1989

Capdevila, J, Gil, L, Orellana, M, Marnett, LJ, Mason, JI, Yadagiri, P, Falck, JR. Inhibitors of cytochrome P-450-dependent arachidonic acid metabolism. Arch Biochem Biophys, 261(2), 257-63, 1988


Postdoctoral Position Available
No

Postdoctoral Position Details
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Updated Date
08/20/2008