Biomedical Research Education & Training
Faculty Member

Emeson, Ronald B., Ph.D.
Professor of Pharmacology
Professor of Psychiatry
Joel G. Hardman Chair in Pharmacology
Professor of Molecular Physiology and Biophysics

Lab Url: N/A

Phone Number: (615) 936-1688, (615) 936-3705

Email Address: ron.emeson@vanderbilt.edu

Emeson, Ronald's picture
Academic history
B.A., The Johns Hopkins University
Ph.D., The University of Colorado Health Sciences Center
Post-doc, University of California, San Diego

Office Address   Mailing Address

8140 Medical Research Building III

8140 MRB III 37232-8548


Research Keywords
molecular neurobiology, gene expression, receptor diversity, neurotransmitters, RNA editing, knockout

Research Specialty
Molecular neurobiology

Research Description
The research interests of our laboratory focus on the field of molecular neurobiology. By integrating the disciplines of peptide biochemistry, cell biology, animal physiology and molecular biology, we hope to gain a better understanding of the cellular and molecular processes underlying neuronal communication in normal and pathophysiological disease states.

Our laboratory is examining the molecular mechanisms involved in the editing of numerous RNA transcripts in the mammalian central nervous system. RNA editing is a post-transcriptional modification in which specific adenosine residues are converted to inosine moieties (A-to-I editing) via the actions of double-stranded RNA-specific adenosine deaminases. As a result of these deamination events, the coding potential of RNAs can be subtly altered to change as little as a single amino acid residue in resultant proteins. In the case of glutamate-gated ion channels, RNA editing can dramatically alter both the ion permeation and electrophysiological properties of these ionotropic receptors. Since glutamate-gated channels are critically involved in processes of excitatory neurotransmission, slight alterations in RNA editing patterns have profound effects upon the normal neurophysiology of the brain. RNA editing events within the 2C-subtype of serotonin receptor can modulate the efficacy by which this seven transmembrane-spanning receptor can couple to its specific intracellular signaling pathways. Since this serotonin receptor has been implicated in a number of neuropsychiatric disease states, including depression and schizophrenia, aberrant RNA editing patterns may play an important role in the etiology of these disorders. Recent studies from our laboratory have also demonstrated that overexpression of ADAR2, a double-stranded RNA-specific adenosine deaminase that catalyzes A-to-I editing results in excessive obesity in transgenic mice, suggesting that misregulation of this post-transcriptional modification can result in severe pathophysiological defects. Current research efforts in the laboratory focus upon the regulation of RNA editing as well as biochemical and bioinformatics strategies for the identification of additional RNAs that are substrates for this type of RNA processing event.

Publications
Hood, JL, Emeson, RB. Editing of Neurotransmitter Receptor and Ion Channel RNAs in the Nervous System. Curr Top Microbiol Immunol, 2011

O''Neil, RT, Emeson, RB. Quantitative analysis of 5HT(2C) receptor RNA editing patterns in psychiatric disorders. Neurobiol Dis, 2011

Morabito, MV, Abbas, AI, Hood, JL, Kesterson, RA, Jacobs, MM, Kump, DS, Hachey, DL, Roth, BL, Emeson, RB. Mice with altered serotonin 2C receptor RNA editing display characteristics of Prader-Willi syndrome. Neurobiol Dis, 39, 169-80, 2010

Morabito, MV, Ulbricht, RJ, O''Neil, RT, Airey, DC, Lu, P, Zhang, B, Wang, L, Emeson, RB. High-throughput multiplexed transcript analysis yields enhanced resolution of 5-hydroxytryptamine 2C receptor mRNA editing profiles. Mol Pharmacol, 77(6), 895-902, 2010

Olaghere da Silva, UB, Morabito, MV, Canal, CE, Airey, DC, Emeson, RB, Sanders-Bush, E. Impact of RNA Editing on Functions of the Serotonin 2C Receptor in vivo. Front Neurosci, 4, 26, 2010 PMCID:2858556

Stefl, R, Oberstrass, FC, Hood, JL, Jourdan, M, Zimmermann, M, Skrisovska, L, Maris, C, Peng, L, Hofr, C, Emeson, RB, Allain, FH. The solution structure of the ADAR2 dsRBM-RNA complex reveals a sequence-specific readout of the minor groove. Cell, 143(2), 225-37, 2010

Jacobs, MM, Fogg, RL, Emeson, RB, Stanwood, GD. ADAR1 and ADAR2 expression and editing activity during forebrain development. Dev Neurosci, 31(3), 223-37, 2009 PMCID:2692045

Morabito, MV, Emeson, RB. RNA editing as a therapeutic target for CNS disorders. Neuropsychopharmacology, 34(1), 246, 2009

Rula, EY, Lagrange, AH, Jacobs, MM, Hu, N, Macdonald, RL, Emeson, RB. Developmental modulation of GABA(A) receptor function by RNA editing. J Neurosci, 28(24), 6196-201, 2008 PMCID:2746000

Rula, EY, Emeson, RB. Mouse Models to Elucidate the Functional Roles of Adenosine-to-Inosine Editing. Methods Enzymol, 424, 333-67, 2007

Singh, M, Kesterson, RA, Jacobs, MM, Joers, JM, Gore, JC, Emeson, RB. Hyperphagia-mediated Obesity in Transgenic Mice Misexpressing the RNA-editing Enzyme ADAR2. J Biol Chem, 282(31), 22448-59, 2007

Thompson, BJ, Washington, MK, Kurre, U, Singh, M, Rula, EY, Emeson, RB. Protective Roles of alpha-Calcitonin and beta-Calcitonin Gene-Related Peptide in Spontaneous and Experimentally Induced Colitis. Dig Dis Sci, 2007

Feng, Y, Sansam, CL, Singh, M, Emeson, RB. Altered RNA editing in mice lacking ADAR2 autoregulation. Mol Cell Biol, 26(2), 480-8, 2006 PMCID:1346902

Huebner, AK, Schinke, T, Priemel, M, Schilling, S, Schilling, AF, Emeson, RB, Rueger, JM, Amling, M. Calcitonin deficiency in mice progressively results in high bone turnover. J Bone Miner Res, 21(12), 1924-34, 2006

Stefl, R, Xu, M, Skrisovska, L, Emeson, RB, Allain, FH. Structure and Specific RNA Binding of ADAR2 Double-Stranded RNA Binding Motifs. Structure, 14(2), 345-55, 2006

Xu, M, Wells, KS, Emeson, RB. Substrate-dependent Contribution of Double-stranded RNA-binding Motifs to ADAR2 Function. Mol Biol Cell, 17(7), 3211-20, 2006 PMCID:1552048

Emeson, RB, Morabito, MV. Food fight: the NPY-serotonin link between aggression and feeding behavior. Sci STKE, 2005(277), pe12, 2005

Sansam, CL and RB Emeson. mRNA Editing. In: Encyclopedia of Life Sciences, John Wiley & Sons, Ltd: Chichester , [doi:10.1038/npg.els.0005041] , 2005

Stefl, R, Skrisovska, L, Xu, M, Emeson, RB, Allain, FH. Resonance assignments of the double-stranded RNA-binding domains of adenosine deaminase acting on RNA 2 (ADAR2). J Biomol NMR, 31(1), 71-2, 2005

Dawson, T. Renee, Sansam, Christopher L., Emeson, Ronald B.. Structure and sequence determinants required for the editing of ADAR2 substrates. J Biol Chem, 279, 4941-4951, 2004

Schinke, T, Liese, S, Priemel, M, Haberland, M, Schilling, AF, Catala-Lehnen, P, Blicharski, D, Rueger, JM, Gagel, RF, Emeson, RB, Amling, M. Decreased bone formation and osteopenia in mice lacking alpha-calcitonin gene-related peptide. J Bone Miner Res, 19(12), 2049-56, 2004

Maison, SF, Emeson, RB, Adams, JC, Luebke, AE, Liberman, MC. Loss of alpha CGRP reduces sound-evoked activity in the cochlear nerve. J Neurophysiol, 90(5), 2941-9, 2003

Sansam, Christopher L, Wells, K Sam, Emeson, Ronald B. Modulation of RNA editing by functional nucleolar sequestration of ADAR2. Proc Natl Acad Sci U S A, 100(24), 14018-23, 2003 PMCID:283538

Berg, KA, Cropper, JD, Niswender, CM, Sanders-Bush, E, Emeson, RB, Clarke, WP. RNA-editing of the 5-HT(2C) receptor alters agonist-receptor-effector coupling specificity. Br J Pharmacol, 134(2), 386-92, 2001 PMCID:1572953

El Meskini, R, Jin, L, Marx, R, Bruzzaniti, A, Lee, J, Emeson, R, Mains, R. A signal sequence is sufficient for green fluorescent protein to be routed to regulated secretory granules. Endocrinology, 142(2), 864-73, 2001

Niswender, C M, Herrick-Davis, K, Dilley, G E, Meltzer, H Y, Overholser, J C, Stockmeier, C A, Emeson, R B, Sanders-Bush, E. RNA editing of the human serotonin 5-HT2C receptor. alterations in suicide and implications for serotonergic pharmacotherapy. Neuropsychopharmacology, 24(5), 478-91, 2001

Gott, JM, Emeson, RB. Functions and mechanisms of RNA editing. Annu Rev Genet, 34, 499-531, 2000

Singh M and Emeson RB. Adenosine to Inosine RNA Editing: Substrates and Consequences. RNA Editing: Frontiers in Molecular Biology. (BL Bass, ed.) Oxford University Press:London, 109-138, 2000

Liu, Y, Emeson, RB, Samuel, CE. Serotonin-2C receptor pre-mRNA editing in rat brain and in vitro by splice site variants of the interferon-inducible double-stranded RNA-specific adenosine deaminase ADAR1. J Biol Chem, 274(26), 18351-8, 1999

Lu, J T, Son, Y J, Lee, J, Jetton, T L, Shiota, M, Moscoso, L, Niswender, K D, Loewy, A D, Magnuson, M A, Sanes, J R, Emeson, R B. Mice lacking alpha-calcitonin gene-related peptide exhibit normal cardiovascular regulation and neuromuscular development. Mol Cell Neurosci, 14(2), 99-120, 1999

Niswender, CM, Copeland, SC, Herrick-Davis, K, Emeson, RB, Sanders-Bush, E. RNA editing of the human serotonin 5-hydroxytryptamine 2C receptor silences constitutive activity. J Biol Chem, 274(14), 9472-8, 1999

Rueter, S M, Dawson, T R, Emeson, R B. Regulation of alternative splicing by RNA editing. Nature, 399(6731), 75-80, 1999

Niswender, C M, Sanders-Bush, E, Emeson, R B. Identification and characterization of RNA editing events within the 5-HT2C receptor. Ann N Y Acad Sci, 861, 38-48, 1998

Rueter S and RB Emeson. Adenosine to Inosine Conversion in mRNA. Modification and editing of RNA: the alteration of RNA structure and function. (H. Grosjean and R. Benne, eds.) ASM Press:East Norwalk, CT, 343-361, 1998

Sanes, J.R., E.D. Apel, R.W. Burgess, R.B. Emeson, G. Feng, M. Gautam, D. Glass, R.M. Grady, E. Krejci, J.W. Lichtman, J.T. Lu, J. Massoulie, J.H. Miner, L.M. Moscoso, Q. Nguyen, M. Nichol, P.G. Noakes, B.L. Patton, Y.J. Son, G.D. Yancopoulos and H. Zhou. Development of the neuromuscular junction: genetic analysis in mice. J Physiol Paris , 92, 167 172, 1998

Bass, BL, Nishikura, K, Keller, W, Seeburg, PH, Emeson, RB, O''Connell, MA, Samuel, CE, Herbert, A. A standardized nomenclature for adenosine deaminases that act on RNA. RNA, 3(9), 947-9, 1997 PMCID:1369539

Breyer, R M, Emeson, R B, Davis, L S, Breyer, M D. Structure and localization of the rabbit prostaglandin EP3 receptor. Adv Exp Med Biol, 400A, 261-8, 1997

Burns, C M, Chu, H, Rueter, S M, Hutchinson, L K, Canton, H, Sanders-Bush, E, Emeson, R B. Regulation of serotonin-2C receptor G-protein coupling by RNA editing. Nature, 387(6630), 303-8, 1997

Canton, H, Emeson, R B, Barker, E L, Backstrom, J R, Lu, J T, Chang, M S, Sanders-Bush, E. Identification, molecular cloning, and distribution of a short variant of the 5-hydroxytryptamine2C receptor produced by alternative splicing. Mol Pharmacol, 50(4), 799-807, 1996

Emeson, R.B. Post-transcriptional regulation of calcitonin gene-related peptide (CGRP) mRNA production. Neurogenic Inflammation. (P. Geppetti and P. Holzer, eds.), CRC Press, Boca Raton, FL, 15-30, 1996

Simpson, L, Emeson, RB. RNA editing. Annu Rev Neurosci, 19, 27-52, 1996

Rueter, S M, Burns, C M, Coode, S A, Mookherjee, P, Emeson, R B. Glutamate receptor RNA editing in vitro by enzymatic conversion of adenosine to inosine. Science, 267(5203), 1491-4, 1995

Breyer, RM, Emeson, RB, Tarng, JL, Breyer, MD, Davis, LS, Abromson, RM, Ferrenbach, SM. Alternative splicing generates multiple isoforms of a rabbit prostaglandin E2 receptor. J Biol Chem, 269(8), 6163-9, 1994

Yeakley, JM, Hedjran, F, Morfin, JP, Merillat, N, Rosenfeld, MG, Emeson, RB. Control of calcitonin/calcitonin gene-related peptide pre-mRNA processing by constitutive intron and exon elements. Mol Cell Biol, 13(10), 5999-6011, 1993 PMCID:364659

Emeson, RB, Yeakley, JM, Hedjran, F, Merillat, N, Lenz, HJ, Rosenfeld, MG. Posttranscriptional regulation of calcitonin/CGRP gene expression. Ann N Y Acad Sci, 657, 18-35, 1992

Rosenfeld, MG, Emeson, RB, Yeakley, JM, Merillat, N, Hedjran, F, Lenz, J, Delsert, C. Calcitonin gene-related peptide: a neuropeptide generated as a consequence of tissue-specific, developmentally regulated alternative RNA processing events. Ann N Y Acad Sci, 657, 1-17, 1992

Emeson, RB, Hedjran, F, Yeakley, JM, Guise, JW, Rosenfeld, MG. Alternative production of calcitonin and CGRP mRNA is regulated at the calcitonin-specific splice acceptor. Nature, 341(6237), 76-80, 1989

Rosenfeld, M, Crenshaw, E B, Emeson, R, Leff, S, Guise, J, Lira, S, Nelson, C, Nelson, C, Russo, A. Transcriptional and post-transcriptional strategies in neuroendocrine gene expression. Dev Biol (N Y 1985), 5, 317-34, 1988

Emeson, RB, Eipper, BA. Characterization of pro-ACTH/endorphin-derived peptides in rat hypothalamus. J Neurosci, 6(3), 837-49, 1986

Emeson, RB. Hypothalamic peptidyl-glycine alpha-amidating monooxygenase: preliminary characterization. J Neurosci, 4(10), 2604-13, 1984


Postdoctoral Position Available
No

Postdoctoral Position Details
N/A

Updated Date
10/21/2010