Biomedical Research Education & Training
Faculty Member

Hanks, Steven K., Ph.D.
Professor of Cell and Developmental Biology, Emeritus

Lab Url: N/A

Phone Number: 615-343-8502

Email Address:

Hanks, Steven's picture
Academic history
B.S., University of Utah, Salt Lake City, UT
Ph.D., U. of Texas, Health Science Ctr., Houston
postdoctoral, Scripps Clinic, La Jolla, CA
postdoctoral, Salk Institute, La Jolla, CA

Office Address   Mailing Address

U-4206 Learned Labs, MRB-III

U-4200 MCN 8240

Research Keywords
cell adhesion and motility, focal adhesion kinase (FAK), integrins, live cell imaging, protein phosphorylation, protein-tyrosine kinases, proteomics, signal transduction, Src-family kinases, tumor invasion and metastasis

Research Description
Our broad research goals are to understand the molecular events that regulate mammalian cell growth, differentiation, and motility, and to determine how these events go awry in cancer and other human diseases. Our primary focus is on tyrosine phosphorylation signaling events regulated by cell-ECM interactions and their roles in cell motility and invasion.

Constancio-Lund, SS, Brabek, J, Hanks, SK. Src transformation of colonic epithelial cells: Enhanced anchorage-independent growth in an Apc(+/min) background. Mol Carcinog, 2008 PMCID:2644448

Kim, W, Seok Kang, Y, Soo Kim, J, Shin, NY, Hanks, SK, Song, WK. The Integrin-coupled Signaling Adaptor p130Cas Suppresses Smad3 Function in Transforming Growth Factor-{beta} Signaling. Mol Biol Cell, 19(5), 2135-46, 2008 PMCID:2366878

Luo, W, Slebos, RJ, Hill, S, Li, M, BraI?bek, J, Amanchy, R, Chaerkady, R, Pandey, A, Ham, AJ, Hanks, SK. Global Impact of Oncogenic Src on a Phosphotyrosine Proteome. J Proteome Res, 2008 PMCID:2579752

Siesser, PM, Meenderink, LM, Ryzhova, L, Michael, KE, Dumbauld, DW, Garc?-a, AJ, Kaverina, I, Hanks, SK. A FAK/Src chimera with gain-of-function properties promotes formation of large peripheral adhesions associated with dynamic actin assembly. Cell Motil Cytoskeleton, 65(1), 25-39, 2008 PMCID:2387247

Liu, G, Li, W, Gao, X, Li, X, J??rgensen, C, Park, HT, Shin, NY, Yu, J, He, ML, Hanks, SK, Wu, JY, Guan, KL, Rao, Y. p130CAS is required for netrin signaling and commissural axon guidance. J Neurosci, 27(4), 957-68, 2007 PMCID:2014516

Frey, MT, Tsai, IY, Russell, TP, Hanks, SK, Wang, YL. Cellular responses to substrate topography: role of myosin II and focal adhesion kinase. Biophys J, 90(10), 3774-82, 2006 PMCID:1440758

Rivera, GM, Antoku, S, Gelkop, S, Shin, NY, Hanks, SK, Pawson, T, Mayer, BJ. Requirement of Nck adaptors for actin dynamics and cell migration stimulated by platelet-derived growth factor B. Proc Natl Acad Sci U S A, 103(25), 9536-41, 2006 PMCID:1476694

Siesser, PM, Hanks, SK. The signaling and biological implications of FAK overexpression in cancer. Clin Cancer Res, 12(11 Pt 1), 3233-7, 2006

Bianchi, M, De Lucchini, S, Marin, O, Turner, DL, Hanks, SK, Villa-Moruzzi, E. Regulation of FAK Ser-722 phosphorylation and kinase activity by GSK3 and PP1 during cell spreading and migration. Biochem J, 391(Pt 2), 359-70, 2005 PMCID:1276935

Br?!bek, J, Constancio, SS, Siesser, PF, Shin, NY, Pozzi, A, Hanks, SK. Crk-associated substrate tyrosine phosphorylation sites are critical for invasion and metastasis of SRC-transformed cells. Mol Cancer Res, 3(6), 307-15, 2005

Cai, S, Bulus, N, Fonseca-Siesser, PM, Chen, D, Hanks, SK, Pozzi, A, Zent, R. CD98 modulates integrin beta1 function in polarized epithelial cells. J Cell Sci, 118(Pt 5), 889-99, 2005

Br?!bek, Jan, Constancio, S?!bata S, Shin, Nah-Young, Pozzi, Ambra, Weaver, Alissa M, Hanks, Steven K. CAS promotes invasiveness of Src-transformed cells. Oncogene, 23(44), 7406-15, 2004

Br?!bek, Jan, Hanks, Steven K. Assaying protein kinase activity. Methods Mol Biol, 284, 79-90, 2004

Fonseca, Priscila M, Shin, Nah-Young, Br?!bek, Jan, Ryzhova, Larisa, Wu, Jiong, Hanks, Steven K. Regulation and localization of CAS substrate domain tyrosine phosphorylation. Cell Signal, 16(5), 621-9, 2004

Hecker, Timothy P, Ding, Qiang, Rege, Tanya A, Hanks, Steven K, Gladson, Candece L. Overexpression of FAK promotes Ras activity through the formation of a FAK/p120RasGAP complex in malignant astrocytoma cells. Oncogene, 23(22), 3962-71, 2004

Kurenova, Elena, Xu, Li-Hui, Yang, Xihui, Baldwin, Albert S, Craven, Rolf J, Hanks, Steven K, Liu, Zheng-Gang, Cance, William G. Focal adhesion kinase suppresses apoptosis by binding to the death domain of receptor-interacting protein. Mol Cell Biol, 24(10), 4361-71, 2004 PMCID:400455

Shin, Nah-Young, Dise, Rebecca S, Schneider-Mergener, Jens, Ritchie, Marylyn D, Kilkenny, Dawn M, Hanks, Steven K. Subsets of the major tyrosine phosphorylation sites in Crk-associated substrate (CAS) are sufficient to promote cell migration. J Biol Chem, 279(37), 38331-7, 2004

Wang, YL, Burridge, K, Dembo, M, Gabbiani, G, Hanks, SK, Hosoya, H, Janmey, P, Karlsson, R, Lindberg, U, Mabuchi, I, Otey, C, Rottner, K, Small, JV, Wang, CL, Zigmond, S. Biomedical research publication system. Science, 303(5666), 1974-6, 2004

Yano, H, Mazaki, Y, Kurokawa, K, Hanks, SK, Matsuda, M, Sabe, H. Roles played by a subset of integrin signaling molecules in cadherin-based cell-cell adhesion. J Cell Biol, 166(2), 283-95, 2004 PMCID:2172299

Hanks, Steven K. Genomic analysis of the eukaryotic protein kinase superfamily: a perspective. Genome Biol, 4(5), 111, 2003 PMCID:156577

Hanks, Steven K, Ryzhova, Larisa, Shin, Nah-Young, Br?!bek, Jan. Focal adhesion kinase signaling activities and their implications in the control of cell survival and motility. Front Biosci, 8, d982-96, 2003

Li, Guochun, Gustafson-Brown, Cindy, Hanks, Steven K, Nason, Katie, Arbeit, Jeffrey M, Pogliano, Kit, Wisdom, Ronald M, Johnson, Randall S. c-Jun is essential for organization of the epidermal leading edge. Dev Cell, 4(6), 865-77, 2003

Miller, Michael A, Ruest, Paul J, Kosinski, Mary, Hanks, Steven K, Greenstein, David. An Eph receptor sperm-sensing control mechanism for oocyte meiotic maturation in Caenorhabditis elegans. Genes Dev, 17(2), 187-200, 2003 PMCID:195972

Torsoni, Adriana S, Constancio, Sabata S, Nadruz, Wilson, Hanks, Steven K, Franchini, Kleber G. Focal adhesion kinase is activated and mediates the early hypertrophic response to stretch in cardiac myocytes. Circ Res, 93(2), 140-7, 2003

Donaldson, John C, Dise, Rebecca S, Ritchie, Marylyn D, Hanks, Steven K. Nephrocystin-conserved domains involved in targeting to epithelial cell-cell junctions, interaction with filamins, and establishing cell polarity. J Biol Chem, 277(32), 29028-35, 2002

Roy, Shyamali, Ruest, Paul J, Hanks, Steven K. FAK regulates tyrosine phosphorylation of CAS, paxillin, and PYK2 in cells expressing v-Src, but is not a critical determinant of v-Src transformation. J Cell Biochem, 84(2), 377-88, 2002

Maru, Y, Hanks, S K, Shibuya, M. The tubulogenic activity associated with an activated form of Flt-1 kinase is dependent on focal adhesion kinase. Biochim Biophys Acta, 1540(2), 147-53, 2001

Ruest, P J, Shin, N Y, Polte, T R, Zhang, X, Hanks, S K. Mechanisms of CAS substrate domain tyrosine phosphorylation by FAK and Src. Mol Cell Biol, 21(22), 7641-52, 2001 PMCID:99935

Wang, H B, Dembo, M, Hanks, S K, Wang, Y. Focal adhesion kinase is involved in mechanosensing during fibroblast migration. Proc Natl Acad Sci U S A, 98(20), 11295-300, 2001 PMCID:58723

Donaldson, J C, Dempsey, P J, Reddy, S, Bouton, A H, Coffey, R J, Hanks, S K. Crk-associated substrate p130(Cas) interacts with nephrocystin and both proteins localize to cell-cell contacts of polarized epithelial cells. Exp Cell Res, 256(1), 168-78, 2000

Ruest, P J, Roy, S, Shi, E, Mernaugh, R L, Hanks, S K. Phosphospecific antibodies reveal focal adhesion kinase activation loop phosphorylation in nascent and mature focal adhesions and requirement for the autophosphorylation site. Cell Growth Differ, 11(1), 41-8, 2000

Sonoda, Y, Matsumoto, Y, Funakoshi, M, Yamamoto, D, Hanks, S K, Kasahara, T. Anti-apoptotic role of focal adhesion kinase (FAK). Induction of inhibitor-of-apoptosis proteins and apoptosis suppression by the overexpression of FAK in a human leukemic cell line, HL-60. J Biol Chem, 275(21), 16309-15, 2000

Owen, J D, Ruest, P J, Fry, D W, Hanks, S K. Induced focal adhesion kinase (FAK) expression in FAK-null cells enhances cell spreading and migration requiring both auto- and activation loop phosphorylation sites and inhibits adhesion-dependent tyrosine phosphorylation of Pyk2. Mol Cell Biol, 19(7), 4806-18, 1999 PMCID:84279

Yamakita, Y, Totsukawa, G, Yamashiro, S, Fry, D, Zhang, X, Hanks, S K, Matsumura, F. Dissociation of FAK/p130(CAS)/c-Src complex during mitosis: role of mitosis-specific serine phosphorylation of FAK. J Cell Biol, 144(2), 315-24, 1999 PMCID:2132894

Zhang, X, Chattopadhyay, A, Ji, Q S, Owen, J D, Ruest, P J, Carpenter, G, Hanks, S K. Focal adhesion kinase promotes phospholipase C-gamma1 activity. Proc Natl Acad Sci U S A, 96(16), 9021-6, 1999 PMCID:17725

Cary, L A, Han, D C, Polte, T R, Hanks, S K, Guan, J L. Identification of p130Cas as a mediator of focal adhesion kinase-promoted cell migration. J Cell Biol, 140(1), 211-21, 1998 PMCID:2132604

Hanks, S K, Polte, T R. Signaling through focal adhesion kinase. Bioessays, 19(2), 137-45, 1997

Polte, T R, Hanks, S K. Complexes of focal adhesion kinase (FAK) and Crk-associated substrate (p130(Cas)) are elevated in cytoskeleton-associated fractions following adhesion and Src transformation. Requirements for Src kinase activity and FAK proline-rich motifs. J Biol Chem, 272(9), 5501-9, 1997

Calalb, M B, Zhang, X, Polte, T R, Hanks, S K. Focal adhesion kinase tyrosine-861 is a major site of phosphorylation by Src. Biochem Biophys Res Commun, 228(3), 662-8, 1996

Withers, B E, Hanks, S K, Fry, D W. Correlations between the expression, phosphotyrosine content and enzymatic activity of focal adhesion kinase, pp125FAK, in tumor and nontransformed cells. Cancer Biochem Biophys, 15(3), 127-39, 1996

Calalb, M B, Polte, T R, Hanks, S K. Tyrosine phosphorylation of focal adhesion kinase at sites in the catalytic domain regulates kinase activity: a role for Src family kinases. Mol Cell Biol, 15(2), 954-63, 1995 PMCID:231984

Hanks, S K, Hunter, T. Protein kinases 6. The eukaryotic protein kinase superfamily: kinase (catalytic) domain structure and classification. FASEB J, 9(8), 576-96, 1995

Kitagawa, M, Higashi, H, Suzuki-Takahashi, I, Segawa, K, Hanks, S K, Taya, Y, Nishimura, S, Okuyama, A. Phosphorylation of E2F-1 by cyclin A-cdk2. Oncogene, 10(2), 229-36, 1995

Polte, T R, Hanks, S K. Interaction between focal adhesion kinase and Crk-associated tyrosine kinase substrate p130Cas. Proc Natl Acad Sci U S A, 92(23), 10678-82, 1995 PMCID:40675

Zhang, X, Wright, C V, Hanks, S K. Cloning of a Xenopus laevis cDNA encoding focal adhesion kinase (FAK) and expression during early development. Gene, 160(2), 219-22, 1995

Gates, R E, King, L E, Hanks, S K, Nanney, L B. Potential role for focal adhesion kinase in migrating and proliferating keratinocytes near epidermal wounds and in culture. Cell Growth Differ, 5(8), 891-9, 1994

Polte, T R, Naftilan, A J, Hanks, S K. Focal adhesion kinase is abundant in developing blood vessels and elevation of its phosphotyrosine content in vascular smooth muscle cells is a rapid response to angiotensin II. J Cell Biochem, 55(1), 106-19, 1994

Schlaepfer, D D, Hanks, S K, Hunter, T, van der Geer, P. Integrin-mediated signal transduction linked to Ras pathway by GRB2 binding to focal adhesion kinase. Nature, 372(6508), 786-91, 1994

Devor, B B, Zhang, X, Patel, S K, Polte, T R, Hanks, S K. Chicken and mouse focal adhesion kinases are similar in structure at their amino termini. Biochem Biophys Res Commun, 190(3), 1084-9, 1993

Gates, R E, Hanks, S K, King, L E. Focal-adhesion components are enriched in ventral membranes isolated from transformed keratinocytes in culture. Biochem J, 289 ( Pt 1), 221-6, 1993 PMCID:1132153

Calalb, M B, Fox, D T, Hanks, S K. Molecular cloning and enzymatic analysis of the rat homolog of 'PhK-gamma T,' an isoform of phosphorylase kinase catalytic subunit. J Biol Chem, 267(3), 1455-63, 1992

Hanks, S K, Calalb, M B, Harper, M C, Patel, S K. Focal adhesion protein-tyrosine kinase phosphorylated in response to cell attachment to fibronectin. Proc Natl Acad Sci U S A, 89(18), 8487-91, 1992 PMCID:49945

Matsushime, H, Ewen, M E, Strom, D K, Kato, J Y, Hanks, S K, Roussel, M F, Sherr, C J. Identification and properties of an atypical catalytic subunit (p34PSK-J3/cdk4) for mammalian D type G1 cyclins. Cell, 71(2), 323-34, 1992

Hanks, S K, Lindberg, R A. Use of degenerate oligonucleotide probes to identify clones that encode protein kinases. Methods Enzymol, 200, 525-32, 1991

Hanks, S K, Quinn, A M. Protein kinase catalytic domain sequence database: identification of conserved features of primary structure and classification of family members. Methods Enzymol, 200, 38-62, 1991

Hanks, S.K. Eukaryotic protein kinases. Curr. Op. Struct. Biol., 1, 369-383, 1991

Hanks, S K. Messenger ribonucleic acid encoding an apparent isoform of phosphorylase kinase catalytic subunit is abundant in the adult testis. Mol Endocrinol, 3(1), 110-6, 1989

Lawton, M A, Yamamoto, R T, Hanks, S K, Lamb, C J. Molecular cloning of plant transcripts encoding protein kinase homologs. Proc Natl Acad Sci U S A, 86(9), 3140-4, 1989 PMCID:287081

Hanks, S K, Armour, R, Baldwin, J H, Maldonado, F, Spiess, J, Holley, R W. Amino acid sequence of the BSC-1 cell growth inhibitor (polyergin) deduced from the nucleotide sequence of the cDNA. Proc Natl Acad Sci U S A, 85(1), 79-82, 1988 PMCID:279485

Hanks, S K, Quinn, A M, Hunter, T. The protein kinase family: conserved features and deduced phylogeny of the catalytic domains. Science, 241(4861), 42-52, 1988

Maldonado, F, Hanks, S K. A cDNA clone encoding human cAMP-dependent protein kinase catalytic subunit C alpha. Nucleic Acids Res, 16(16), 8189-90, 1988 PMCID:338534

Hanks, S K. Homology probing: identification of cDNA clones encoding members of the protein-serine kinase family. Proc Natl Acad Sci U S A, 84(2), 388-92, 1987 PMCID:304212

Hanks, S K, Riggs, M G. Selective insolubility of active hsp70 gene chromatin. Biochim Biophys Acta, 867(3), 124-34, 1986

Mitchell, R L, Hanks, S K, Verma, I M. Proto-oncogene fos: an inducible multifaceted gene. Symp Fundam Cancer Res, 39, 99-113, 1986

Brown, D B, Hanks, S K, Murphy, E C, Rao, P N. Early initiation of DNA synthesis in G1 phase HeLa cells following fusion with red cell ghosts loaded with S-phase cell extracts. Exp Cell Res, 156(1), 251-9, 1985

Gollin, S M, Wray, W, Hanks, S K, Hittelman, W N, Rao, P N. The ultrastructural organization of prematurely condensed chromosomes. J Cell Sci Suppl, 1, 203-21, 1984

Hanks, S K, Gollin, S M, Rao, P N, Wray, W, Hittelman, W N. Cell cycle-specific changes in the ultrastructural organization of prematurely condensed chromosomes. Chromosoma, 88(5), 333-42, 1983

Hanks, S K, Rodriguez, L V, Rao, P N. Relationship between histone phosphorylation and premature chromosome condensation. Exp Cell Res, 148(2), 293-302, 1983

Hanks, S K, Brown, D B, Rao, P N. Induction of premature chromosome condensation at high frequency following polyethylene glycol-mediated fusion of lectin-bound cells. Exp Cell Res, 138(1), 215-9, 1982

Hanks, S K, Rao, P N. Initiation of DNA synthesis in the prematurely condensed chromosomes of G1 cells. J Cell Biol, 87(1), 285-91, 1980 PMCID:2110714

Rao, P N, Hanks, S K. Chromatin structure during the prereplicative phases in the life cycle of mammalian cells. Cell Biophys, 2(4), 327-37, 1980

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