Biomedical Research Education & Training
Faculty Member

Ong, David E., Ph.D.
Professor of Biochemistry, Emeritus

Lab Url: http://

Phone Number: 615-322-4343

Email Address: david.e.ong@vanderbilt.edu

Ong, David's picture
Academic history
B.A., Wabash College, Crawfordsville, IN
Ph.D., Yale U., New Haven, CT
Postdoc, Vanderbilt U., Nashville, TN

Office Address   Mailing Address

610 Robinson Research Building Vanderbilt University
Nashville, TN 37232-0146

610 RRB 0146


Research Keywords
Retinoids,Cancer,Reproduction,Biochemistry,Endocrinology

Research Specialty
Vitamin A (Retinoid) binding proteins; Retinoid metabolism and function; Retinoid regulated gene expression.

Research Description
Vitamin A is an essential micronutrient that provides not only the signaling molecule essential for vision (11-cis-retinal) but also other retinoids. For example, retinoic acid activates a family of nuclear proteins (the Retinoic Acid Receptors a, b, and g) that direct transcription of genes important for morphological development, proper cellular differentiation and growth control. One member of the RAR group is found in virtually every cell type examined, indicating the essential role that retinoids play in proper bodily function. Remarkably, we still know little about how, where, and when the retinoic acid that activates these receptors is produced. This is currently the major interest of this laboratory. Central to retinoic acid production are a family of carrier proteins, found within certain cells (the cellular retinoid binding proteins), and the plasma carrier protein for the parent retinoid, retinol (retinol binding protein). We have demonstrated that these carrier proteins are directly recognized by other cellular components (enzymes and membrane transporters) to allow the proper delivery and metabolism of retinoids. Our work focuses on defining these interactions at the molecular level as well as using such interactions to find previously unknown enzymes essential for the functions of vitamin A. Techniques used range from site-directed mutagenesis of these proteins to study their interaction with both ligand and enzymes, classical work in enzymology, molecular biological approaches for the study of regulation, as well as immunolocalization at both the light and electron microscopic level of these players to define sites of retinoid action.
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Publications
Everts, HB, Sundberg, JP, Ong, DE. Immunolocalization of retinoic acid biosynthesis systems in selected sites in rat. Exp Cell Res, 308(2), 309-19, 2005

Swift, LL, Jovanovska, A, Kakkad, B, Ong, DE. Microsomal triglyceride transfer protein expression in mouse intestine. Histochem Cell Biol, 123(4-5), 475-82, 2005

Swift, LL, Kakkad, B, Boone, C, Jovanovska, A, Jerome, WG, Mohler, PJ, Ong, DE. Microsomal triglyceride transfer protein expression in adipocytes: a new component in fat metabolism. FEBS Lett, 579(14), 3183-9, 2005

Everts, H.B., King Jr., L.E., Sundberg, J.P. and Ong, D.E. . Hair Cycle specific Immunolocalization of Retinoic Acid-synthesizing Enzymes Aldh1a2 and Aldh1a3 Indicate Complex Regulation.. J. Invest. Derm., 123, 258-263, 2004

Everts, HB, King, LE, Sundberg, JP, Ong, DE. Hair cycle-specific immunolocalization of retinoic acid synthesizing enzymes Aldh1a2 and Aldh1a3 indicate complex regulation. J Invest Dermatol, 123(2), 258-63, 2004

Li, X-H, Kakkad, B. and Ong, DE . Estrogen Directly Induces Expression of Retinoic Acid Biosynthetic Enzymes, Compartmentalized Between the Epithelium and Underlying Stromal Cells in Rat Uterus.. Endocrinology, 145, 4756-4762, 2004

Li, XH, Kakkad, B, Ong, DE. Estrogen directly induces expression of retinoic acid biosynthetic enzymes, compartmentalized between the epithelium and underlying stromal cells in rat uterus. Endocrinology, 145(10), 4756-62, 2004

Fouch??court, Sophie, Lareyre, Jean-Jacques, Chaurand, Pierre, DaGue, Beverly B, Suzuki, Kichiya, Ong, David E, Olson, Gary E, Matusik, Robert J, Caprioli, Richard M, Orgebin-Crist, Marie-Claire. Identification, immunolocalization, regulation, and postnatal development of the lipocalin EP17 (epididymal protein of 17 kilodaltons) in the mouse and rat epididymis. Endocrinology, 144, 887-900, 2003

Li, Xiao-Hong, Ong, David E. Cellular retinoic acid-binding protein II gene expression is directly induced by estrogen, but not retinoic acid, in rat uterus. J Biol Chem, 378, 35819-35825, 2003

Lucey, Edgar C, Goldstein, Ronald H, Breuer, Raphael, Rexer, Brent N, Ong, David E, Snider, Gordon L. Retinoic acid does not affect alveolar septation in adult FVB mice with elastase-induced emphysema. Respiration, 70, 200-5, 2003

Swift, Larry L., Zhu, Mei-Ying, Kakkad, Bharati, Jovanovska, Aneta, Neely, M. Diana, Valyi-Nagy, Klara, Roberts, Richard L., Ong, David E., Jerome, W. Gray. Subcellular localization of microsomal triglyceride transfer protein (MTP). J Lipid Res, 44, 1841-1849, 2003

Rexer, Brent N, Ong, David E. A novel short-chain alcohol dehydrogenase from rats with retinol dehydrogenase activity, cyclically expressed in uterine epithelium. Biol Reprod, 67, 1555-64, 2002

Rexer, B., Zheng, W.L. and Ong, D.E. (2001) Retinoic Acid synthesis by normal human breast epithelium is via Aldehyde Dehydrogenase 6, absent in MCF-7 cells. Cancer Research 61, 7065-7070.

Lareyre, J J, Winfrey, V P, Kasper, S, Ong, D E, Matusik, R J, Olson, G E, Orgebin-Crist, M C. Gene duplication gives rise to a new 17-kilodalton lipocalin that shows epididymal region-specific expression and testicular factor(s) regulation. Endocrinology, 142(3), 1296-308, 2001

Rexer, B N, Zheng, W L, Ong, D E. Retinoic acid biosynthesis by normal human breast epithelium is via aldehyde dehydrogenase 6, absent in MCF-7 cells. Cancer Res, 61(19), 7065-70, 2001

Mira-Y-Lopez, R, Zheng, W L, Kuppumbatti, Y S, Rexer, B, Jing, Y, Ong, D E. Retinol conversion to retinoic acid is impaired in breast cancer cell lines relative to normal cells. J Cell Physiol, 185(2), 302-9, 2000

Mira-y-Lopez, R., Zheng, W.L., Kuppumbatti, Y.S., Rexer, B., Jing, Y. and Ong, D.E. Retinol Conversion To Retinoic Acid Is Impaired In Breast Cancer Cell Lines. J. Cell Physiol., 185, 302-309 , 2000

Newcomer, M E, Ong, D E. Plasma retinol binding protein: structure and function of the prototypic lipocalin. Biochim Biophys Acta, 1482(1-2), 57-64, 2000

Ong, D E, Newcomer, M E, Lareyre, J J, Orgebin-Crist, M C. Epididymal retinoic acid-binding protein. Biochim Biophys Acta, 1482(1-2), 209-17, 2000

Ruff, S J, Ong, D E. Cellular retinoic acid binding protein is associated with mitochondria. FEBS Lett, 487(2), 282-6, 2000

Ruff, S.J. and Ong, D.E. Cellular Retinoic Acid Binding Protein is Associated with Mitochondria. FEBS Lett, 487, 282-286, 2000

Zheng, W L, Sierra-Rivera, E, Luan, J, Osteen, K G, Ong, D E. Retinoic acid synthesis and expression of cellular retinol-binding protein and cellular retinoic acid-binding protein type II are concurrent with decidualization of rat uterine stromal cells. Endocrinology, 141(2), 802-8, 2000

Lareyre, J J, Thomas, T Z, Zheng, W L, Kasper, S, Ong, D E, Orgebin-Crist, M C, Matusik, R J. A 5-kilobase pair promoter fragment of the murine epididymal retinoic acid-binding protein gene drives the tissue-specific, cell-specific, and androgen-regulated expression of a foreign gene in the epididymis of transgenic mice. J Biol Chem, 274(12), 8282-90, 1999

Layre, J-J., Thomas, T.Z., Zheng, W.L., Kasper, S., Ong, D.E., Matusik, R.J., and Orgebin-Crist, M-C. A 5-kb promoter fragment of the murine epididymal retinoic acid-binding protein (mE-RABP) drives the tissue-specific, cell-specific, and androgen-regulated expression of a foreign gene in the epididymis of transgenic mice. J. Biol. Chem., 274, 8282-8290, 1999

Tr??en, G, Eskild, W, Fromm, S H, De Luca, L M, Ong, D E, Wardlaw, S A, Reppe, S, Blomhoff, R. Vitamin A-sensitive tissues in transgenic mice expressing high levels of human cellular retinol-binding protein type I are not altered phenotypically. J Nutr, 129(9), 1621-7, 1999

Zheng, W L, Bucco, R A, Sierra-Rievera, E, Osteen, K G, Melner, M H, Ong, D E. Synthesis of retinoic acid by rat ovarian cells that express cellular retinoic acid-binding protein-II. Biol Reprod, 60(1), 110-4, 1999

Zheng, W.L., Bucco, R.A., Sierra-Rivera, E., Osteen, K.G., Melner, M.H., and Ong, D.E. Synthesis of retinoic acid by CRABP(II) expressing cells of rat ovary. Biol. Reprod., 60, 110-114, 1999

Jamison, R S, Newcomer, M E, Ong, D E. Detection of conformational changes in cellular retinoid-binding proteins by limited proteolysis. Methods Mol Biol, 89, 165-76, 1998

Kingma, P B, Bok, D, Ong, D E. Bovine epidermal fatty acid-binding protein: determination of ligand specificity and cellular localization in retina and testis. Biochemistry, 37(10), 3250-7, 1998

Kingma, P.B., Bok, D. and Ong, D.E. Bovine epidermal fatty acid-binding protein: determination of ligand specificity and cellular localization in retina and testis. Biochemistry, 32, 3250-3257, 1998

Lareyre, J J, Matt??i, M G, Kasper, S, Newcomer, M E, Ong, D E, Matusik, R J, Orgebin-Crist, M C. Structure and putative function of a murine epididymal retinoic acid-binding protein (mE-RABP). J Reprod Fertil Suppl, 53, 59-65, 1998

Lareyre, J J, Matt??i, M G, Kasper, S, Ong, D E, Matusik, R J, Orgebin-Crist, M C. Genomic organization and chromosomal localization of the murine epididymal retinoic acid-binding protein (mE-RABP) gene. Mol Reprod Dev, 50(4), 387-95, 1998

Lareyre, J J, Zheng, W L, Zhao, G Q, Kasper, S, Newcomer, M E, Matusik, R J, Ong, D E, Orgebin-Crist, M C. Molecular cloning and hormonal regulation of a murine epididymal retinoic acid-binding protein messenger ribonucleic acid. Endocrinology, 139(6), 2971-81, 1998

Layre, J-J., Mattei, M.-G., Kasper, S., Ong, D.E., Matusik, R.J., and Orgebin-Crist, M-C. Genomic organization and chromosomal localization of the murine epididymal retinoic acid-binding protein (mE-RABP) Gene. Molec. Reprod. Develop, 50, 387-395, 1998

Layre, J-J., Zheng, W.L., Zhao, G-Q, Kasper, S., Newcomer, M.E., Matusik, R.J., Ong, D.E., and Orgebin-Crist, M-C. Molecular cloning and hormonal regulation of a murine epididymal retinoic acid-binding protein mRNA. Endocrinology, 139, 2971-2981, 1998

Newcomer, M E, Jamison, R S, Ong, D E. Structure and function of retinoid-binding proteins. Subcell Biochem, 30, 53-80, 1998

Yamamoto, M, Dr??ger, U C, Ong, D E, McCaffery, P. Retinoid-binding proteins in the cerebellum and choroid plexus and their relationship to regionalized retinoic acid synthesis and degradation. Eur J Biochem, 257(2), 344-50, 1998

Yamamoto, M., Dr?ger, U.C., Ong, D.E., and McCaffery, P. Retinoid binding proteins in the cerebellum and choroid plexus and their relationship to regionalized retinoic acid synthesis and degradation. Euro. J. Biochem, 257, 344-350, 1998

Zheng, W L, Ong, D E. Spatial and temporal patterns of expression of cellular retinol-binding protein and cellular retinoic acid-binding proteins in rat uterus during early pregnancy. Biol Reprod, 58(4), 963-70, 1998

Zheng, W. L. and Ong, D. E. Spatial and temporal pattern of expression of CRBP and CRABPs in rat uterus during early pregnancy. Biol. Reprod, 58, 963-970, 1998

B?vik, C., Ward, S. J. and Ong, D. E. Identification of a mechanism to localize generation of retinoic acid in rat embryos. Mechanisms Of Development, 69, 155-167, 1997

Bucco, R A, Zheng, W L, Davis, J T, Sierra-Rivera, E, Osteen, K G, Chaudhary, A K, Ong, D E. Cellular retinoic acid-binding protein(II) presence in rat uterine epithelial cells correlates with their synthesis of retinoic acid. Biochemistry, 36(13), 4009-14, 1997

Bucco, R.A., Zheng, W.L., Davis, J.T., Sierra-Rivera, E., Osteen, K.G., Chaudhary, A.K., and Ong, D.E. Cellular retinoic acid-binding protein(II) presence in rat uterine epithelial cells correlates with their synthesis of retinoic acid. Biochemistry, 36, 4009-4014, 1997

B?Yvik, C, Ward, S J, Ong, D E. Identification of a mechanism to localize generation of retinoic acid in rat embryos. Mech Dev, 69(1-2), 155-67, 1997

Dew, S E, Ong, D E. Absorption of retinol from the retinol:retinol-binding protein complex by small intestinal gut sheets from the rat. Arch Biochem Biophys, 338(2), 233-6, 1997

Dew, S. E. and Ong, D. E. Absorption of retinol from the retinol:retinol binding protein complex by small intestinal gut sheets from the rat. Arch. Biochem. Biophys, 338, 233-236, 1997

Ward, S J, Chambon, P, Ong, D E, B?Yvik, C. A retinol-binding protein receptor-mediated mechanism for uptake of vitamin A to postimplantation rat embryos. Biol Reprod, 57(4), 751-5, 1997

Ward, S. J., Chambon, P., Ong, D. E., and B'vik, C. A retinol-binding protein receptor mediated mechanism for embryonic uptake of Vitamin A. Biol. Reprod, 57, 751-755, 1997

Wardlaw, S A, Bucco, R A, Zheng, W L, Ong, D E. Variable expression of cellular retinol- and cellular retinoic acid-binding proteins in the rat uterus and ovary during the estrous cycle. Biol Reprod, 56(1), 125-32, 1997

Wardlaw, S.A., Bucco, R.A., Zheng, W.L., and Ong, D.E. Variable expression of cellular retinol- and cellular retinoic acid-binding proteins in the rat uterus and ovary during the estrous cycle. Biol. Reprod, 56, 125-132, 1997

Bucco, R A, Zheng, W L, Wardlaw, S A, Davis, J T, Sierra-Rivera, E, Osteen, K G, Melner, M H, Kakkad, B P, Ong, D E. Regulation and localization of cellular retinol-binding protein, retinol-binding protein, cellular retinoic acid-binding protein (CRABP), and CRABP II in the uterus of the pseudopregnant rat. Endocrinology, 137(7), 3111-22, 1996

Bucco, R.A., Zheng, W.L., Wardlaw, S.A., Davis, J.T., Sierra-Rivera, E., Osteen, K., Melner, M.H., Kakkad, B. and Ong, D.E. Regulation and localization of cellular retinol-binding protein, retinol-binding protein, cellular retinoic acid-binding protein (CRABP), and CRABP(II) in the uterus of the pseudopregnant rat. Endocrinology, 137, 3111-3122, 1996

Zheng, W L, Bucco, R A, Schmitt, M C, Wardlaw, S A, Ong, D E. Localization of cellular retinoic acid-binding protein (CRABP) II and CRABP in developing rat testis. Endocrinology, 137(11), 5028-35, 1996

Zheng, W.L., Bucco, R.A., Schmitt, M. C., Wardlaw, S.A., and Ong, D.E. Localization of CRABP(II) and CRABP in developing rat testis. Endocrinology, 137, 5028-5035, 1996

Bucco, R A, Melner, M H, Gordon, D S, Leers-Sucheta, S, Ong, D E. Inducible expression of cellular retinoic acid-binding protein II in rat ovary: gonadotropin regulation during luteal development. Endocrinology, 136(6), 2730-40, 1995

Bucco, R.A., Melner, M.H., Gordon, D.S., Leers-Sucheta, S. and Ong, D.E. Inducible expression of cellular retinoic acid-binding protein II in rat ovary: gonadotropin regulation during luteal development. Endocrinology, 136, 2730-2740, 1995

Davis, J T, Ong, D E. Retinol processing by the peritubular cell from rat testis. Biol Reprod, 52(2), 356-64, 1995

Davis, J.T. and Ong, D.E. Retinol processing by the peritubular cell from rat testis. Biol. Reprod., 52, 356-364, 1995

Jamison, R S, Kakkad, B, Ebert, D H, Newcomer, M E, Ong, D E. Test of the contribution of an amino-aromatic hydrogen bond to protein function. Biochemistry, 34(35), 11128-32, 1995

Jamison, R.S., Kakkad, B., Ebert, D.H., Newcomer, M.E., and Ong, D.E. Test of the contribution of an amino-aromatic hydrogen bond to protein function. Biochemistry, 34, 11128-11132, 1995

Dew, S E, Ong, D E. Specificity of the retinol transporter of the rat small intestine brush border. Biochemistry, 33(40), 12340-5, 1994

Jamison, R S, Newcomer, M E, Ong, D E. Cellular retinoid-binding proteins: limited proteolysis reveals a conformational change upon ligand binding. Biochemistry, 33(10), 2873-9, 1994

Ong, D E. Cellular transport and metabolism of vitamin A: roles of the cellular retinoid-binding proteins. Nutr Rev, 52(2 Pt 2), S24-31, 1994

Ong, D E, Davis, J T, O'Day, W T, Bok, D. Synthesis and secretion of retinol-binding protein and transthyretin by cultured retinal pigment epithelium. Biochemistry, 33(7), 1835-42, 1994

Rigtrup, K M, Kakkad, B, Ong, D E. Purification and partial characterization of a retinyl ester hydrolase from the brush border of rat small intestine mucosa: probable identity with brush border phospholipase B. Biochemistry, 33(9), 2661-6, 1994

Rigtrup, K M, McEwen, L R, Said, H M, Ong, D E. Retinyl ester hydrolytic activity associated with human intestinal brush border membranes. Am J Clin Nutr, 60(1), 111-6, 1994

Schmitt, M C, Jamison, R S, Orgebin-Crist, M C, Ong, D E. A novel, testis-specific member of the cellular lipophilic transport protein superfamily, deduced from a complimentary deoxyribonucleic acid clone. Biol Reprod, 51(2), 239-45, 1994

Dew, S E, Wardlaw, S A, Ong, D E. Effects of pharmacological retinoids on several vitamin A-metabolizing enzymes. Cancer Res, 53(13), 2965-9, 1993

Herr, F M, Wardlaw, S A, Kakkad, B, Albrecht, A, Quick, T C, Ong, D E. Intestinal vitamin A metabolism: coordinate distribution of enzymes and CRBP(II). J Lipid Res, 34(9), 1545-54, 1993

Newcomer, M E, Pappas, R S, Ong, D E. X-ray crystallographic identification of a protein-binding site for both all-trans- and 9-cis-retinoic acid. Proc Natl Acad Sci U S A, 90(19), 9223-7, 1993 PMCID:47535

Ong, D E. Retinoid metabolism during intestinal absorption. J Nutr, 123(2 Suppl), 351-5, 1993

Pappas, R S, Newcomer, M E, Ong, D E. Endogenous retinoids in rat epididymal tissue and rat and human spermatozoa. Biol Reprod, 48(2), 235-47, 1993

Schmitt, M C, Ong, D E. Expression of cellular retinol-binding protein and lecithin-retinol acyltransferase in developing rat testis. Biol Reprod, 49(5), 972-9, 1993

Davis, J T, Ong, D E. Synthesis and secretion of retinol-binding protein by cultured rat Sertoli cells. Biol Reprod, 47(4), 528-33, 1992

Herr, F M, Ong, D E. Differential interaction of lecithin-retinol acyltransferase with cellular retinol binding proteins. Biochemistry, 31(29), 6748-55, 1992

Inagami, S, Ong, D E. Purification and partial characterization of cellular retinol-binding protein, type two, from human small intestine. J Nutr, 122(3), 450-6, 1992

Rankin, T L, Ong, D E, Orgebin-Crist, M C. The 18-kDa mouse epididymal protein (MEP 10) binds retinoic acid. Biol Reprod, 46(5), 767-71, 1992

Rigtrup, K M, Ong, D E. A retinyl ester hydrolase activity intrinsic to the brush border membrane of rat small intestine. Biochemistry, 31(11), 2920-6, 1992

Ong, D E, Lucas, P C, Kakkad, B, Quick, T C. Ontogeny of two vitamin A-metabolizing enzymes and two retinol-binding proteins present in the small intestine of the rat. J Lipid Res, 32(9), 1521-7, 1991

Finlay, J A, Strom, M, Ong, D E, DeLuca, H F. Regulation of cellular retinol binding protein type II by 1,25-dihydroxyvitamin D3. Biochemistry, 29(20), 4914-21, 1990

MacDonald, P N, Bok, D, Ong, D E. Localization of cellular retinol-binding protein and retinol-binding protein in cells comprising the blood-brain barrier of rat and human. Proc Natl Acad Sci U S A, 87(11), 4265-9, 1990 PMCID:54089

MacDonald, P N, Ong, D E. Assay of lecithin-retinol acyltransferase. Methods Enzymol, 189, 450-9, 1990

Maden, M, Ong, D E, Chytil, F. Retinoid-binding protein distribution in the developing mammalian nervous system. Development, 109(1), 75-80, 1990

Newcomer, M E, Ong, D E. Purification and crystallization of a retinoic acid-binding protein from rat epididymis. Identity with the major androgen-dependent epididymal proteins. J Biol Chem, 265(22), 12876-9, 1990

Quick, T C, Ong, D E. Vitamin A metabolism in the human intestinal Caco-2 cell line. Biochemistry, 29(50), 11116-23, 1990

Maden, M, Ong, D E, Summerbell, D, Chytil, F. The role of retinoid-binding proteins in the generation of pattern in the developing limb, the regenerating limb and the nervous system. Development, 107 Suppl, 109-19, 1989

Maden, M, Ong, D E, Summerbell, D, Chytil, F, Hirst, E A. Cellular retinoic acid-binding protein and the role of retinoic acid in the development of the chick embryo. Dev Biol, 135(1), 124-32, 1989

Quick, T C, Ong, D E. Levels of cellular retinol-binding proteins in the small intestine of rats during pregnancy and lactation. J Lipid Res, 30(7), 1049-54, 1989

Rubin, D C, Ong, D E, Gordon, J I. Cellular differentiation in the emerging fetal rat small intestinal epithelium: mosaic patterns of gene expression. Proc Natl Acad Sci U S A, 86(4), 1278-82, 1989 PMCID:286671

Said, H M, Ong, D E, Shingleton, J L. Intestinal uptake of retinol: enhancement by bovine milk beta-lactoglobulin. Am J Clin Nutr, 49(4), 690-4, 1989

Schaefer, W H, Kakkad, B, Crow, J A, Blair, I A, Ong, D E. Purification, primary structure characterization, and cellular distribution of two forms of cellular retinol-binding protein, type II from adult rat small intestine. J Biol Chem, 264(7), 4212-21, 1989

Shingleton, J L, Skinner, M K, Ong, D E. Retinol esterification in Sertoli cells by lecithin-retinol acyltransferase. Biochemistry, 28(25), 9647-53, 1989

Shingleton, J L, Skinner, M K, Ong, D E. Characteristics of retinol accumulation from serum retinol-binding protein by cultured Sertoli cells. Biochemistry, 28(25), 9641-7, 1989

Kakkad, B P, Ong, D E. Reduction of retinaldehyde bound to cellular retinol-binding protein (type II) by microsomes from rat small intestine. J Biol Chem, 263(26), 12916-9, 1988

MacDonald, P N, Ong, D E. A lecithin:retinol acyltransferase activity in human and rat liver. Biochem Biophys Res Commun, 156(1), 157-63, 1988

MacDonald, P N, Ong, D E. Evidence for a lecithin-retinol acyltransferase activity in the rat small intestine. J Biol Chem, 263(25), 12478-82, 1988

Maden, M, Ong, D E, Summerbell, D, Chytil, F. Spatial distribution of cellular protein binding to retinoic acid in the chick limb bud. Nature, 335(6192), 733-5, 1988

Ong, D E, Chytil, F. Presence of novel retinoic acid-binding proteins in the lumen of rat epididymis. Arch Biochem Biophys, 267(2), 474-8, 1988

Ong, D E, MacDonald, P N, Gubitosi, A M. Esterification of retinol in rat liver. Possible participation by cellular retinol-binding protein and cellular retinol-binding protein II. J Biol Chem, 263(12), 5789-96, 1988

Chytil, F, Ong, D E. Intracellular vitamin A--binding proteins. Annu Rev Nutr, 7, 321-35, 1987

Crow, J A, Ong, D E, Chytil, F. Specificity of cellular retinol-binding protein in the transfer of retinol to nuclei and chromatin. Arch Biochem Biophys, 254(1), 372-5, 1987

Levin, M S, Li, E, Ong, D E, Gordon, J I. Comparison of the tissue-specific expression and developmental regulation of two closely linked rodent genes encoding cytosolic retinol-binding proteins. J Biol Chem, 262(15), 7118-24, 1987

Li, E, Locke, B, Yang, N C, Ong, D E, Gordon, J I. Characterization of rat cellular retinol-binding protein II expressed in Escherichia coli. J Biol Chem, 262(28), 13773-9, 1987

MacDonald, P N, Ong, D E. Binding specificities of cellular retinol-binding protein and cellular retinol-binding protein, type II. J Biol Chem, 262(22), 10550-6, 1987

Ong, D E. Cellular retinoid-binding proteins. Arch Dermatol, 123(12), 1693-1695a, 1987

Ong, D E, Am??d??e-Manesme, O. Liver levels of vitamin A and cellular retinol-binding protein for patients with biliary atresia. Hepatology, 7(2), 253-6, 1987

Ong, D E, Kakkad, B, MacDonald, P N. Acyl-CoA-independent esterification of retinol bound to cellular retinol-binding protein (type II) by microsomes from rat small intestine. J Biol Chem, 262(6), 2729-36, 1987

Ong, D E, Page, D L. Cellular retinol-binding protein (type two) is abundant in human small intestine. J Lipid Res, 28(6), 739-45, 1987

Ong, D E, Takase, S, Chytil, F. Cellular vitamin A-binding proteins in the testis. Ann N Y Acad Sci, 513, 172-8, 1987

Li, E, Demmer, L A, Sweetser, D A, Ong, D E, Gordon, J I. Rat cellular retinol-binding protein II: use of a cloned cDNA to define its primary structure, tissue-specific expression, and developmental regulation. Proc Natl Acad Sci U S A, 83(16), 5779-83, 1986 PMCID:386378

Ong, D E, Page, D L. Quantitation of cellular retinol-binding protein in human organs. Am J Clin Nutr, 44(3), 425-30, 1986

Porter, S B, Ong, D E, Chytil, F. Vitamin A status affects chromatin structure. Int J Vitam Nutr Res, 56(1), 11-20, 1986

Takase, S, Ong, D E, Chytil, F. Transfer of retinoic acid from its complex with cellular retinoic acid-binding protein to the nucleus. Arch Biochem Biophys, 247(2), 328-34, 1986

Crow, J A, Ong, D E. Cell-specific immunohistochemical localization of a cellular retinol-binding protein (type two) in the small intestine of rat. Proc Natl Acad Sci U S A, 82(14), 4707-11, 1985 PMCID:390973

Liau, G, Ong, D E, Chytil, F. Partial characterization of nuclear binding sites for retinol delivered by cellular retinol binding protein. Arch Biochem Biophys, 237(2), 354-60, 1985

Ong, D E. Vitamin A-binding proteins. Nutr Rev, 43(8), 225-32, 1985

Porter, S B, Ong, D E, Chytil, F, Orgebin-Crist, M C. Localization of cellular retinol-binding protein and cellular retinoic acid-binding protein in the rat testis and epididymis. J Androl, 6(3), 197-212, 1985

Bok, D, Ong, D E, Chytil, F. Immunocytochemical localization of cellular retinol binding protein in the rat retina. Invest Ophthalmol Vis Sci, 25(8), 877-83, 1984

Miller, J H, Gates, R E, Ong, D E, King, L E. A miniature molecular-sieving column assay for cytoplasmic vitamin A-binding proteins. Anal Biochem, 139(1), 104-14, 1984

Ong, D E. A novel retinol-binding protein from rat. Purification and partial characterization. J Biol Chem, 259(3), 1476-82, 1984

Chytil, F, Ong, D E. Cellular retinol- and retinoic acid-binding proteins. Adv Nutr Res, 5, 13-29, 1983

Ong, D E, Chytil, F. Vitamin A and cancer. Vitam Horm, 40, 105-44, 1983

Porter, S B, Fraker, L D, Chytil, F, Ong, D E. Localization of cellular retinol-binding protein in several rat tissues. Proc Natl Acad Sci U S A, 80(21), 6586-90, 1983 PMCID:391214

Ong, D E. Purification and partial characterization of cellular retinol-binding protein from human liver. Cancer Res, 42(3), 1033-7, 1982

Ong, D E, Crow, J A, Chytil, F. Radioimmunochemical determination of cellular retinol- and cellular retinoic acid-binding proteins in cytosols of rat tissues. J Biol Chem, 257(22), 13385-9, 1982

Ong, D E, Goodwin, W J, Jesse, R H, Griffin, A C. Presence of cellular retinol and retinoic acid-binding proteins in epidermoid carcinoma of the oral cavity and oropharynx. Cancer, 49(7), 1409-12, 1982

Kylberg, H K, Ong, D E, Chytil, F. Cellular retinol binding protein during postanal development of the rat small intestine. Biol Neonate, 39(1-2), 100-4, 1981

Liau, G, Ong, D E, Chytil, F. Interaction of the retinol/cellular retinol-binding protein complex with isolated nuclei and nuclear components. J Cell Biol, 91(1), 63-8, 1981 PMCID:2111952

Ong, D E, Chytil, F. Immunochemical studies on cellular vitamin A-binding proteins. Ann N Y Acad Sci, 359, 415-7, 1981

Lotan, R, Ong, D E, Chytil, F. Comparison of the level of cellular retinoid-binding proteins and susceptibility to retinoid-induced growth inhibition of various neoplastic cell lines. J Natl Cancer Inst, 64(5), 1259-62, 1980

Chytil, F, Ong, D E. Cellular retinol- and retinoic acid-binding proteins in vitamin A action. Fed Proc, 38(11), 2510-4, 1979

Ong, D E, Chytil, F. Immunochemical comparison of vitamin A binding proteins of rat. J Biol Chem, 254(18), 8733-5, 1979

Takase, S, Ong, D E, Chytil, F. Cellular retinol-binding protein allows specific interaction of retinol with the nucleus in vitro. Proc Natl Acad Sci U S A, 76(5), 2204-8, 1979 PMCID:383566

Chytil, F, Ong, D E. Cellular vitamin A binding proteins. Vitam Horm, 36, 1-32, 1978

Ong, D E, Chytil, F. Cellular retinol-binding protein from rat liver. Purification and characterization. J Biol Chem, 253(3), 828-32, 1978

Ong, D E, Chytil, F. Cellular retinoic acid-binding protein from rat testis. Purification and characterization. J Biol Chem, 253(13), 4551-4, 1978

Ong, D E, Markert, C, Chiu, J F. Cellular binding proteins for vitamin A in colorectal adenocarcinoma of rat. Cancer Res, 38(12), 4422-6, 1978

Chytil, F, Ong, D E. Mediation of retinoic acid-induced growth and anti-tumour activity. Nature, 260(5546), 49-51, 1976

Ong, D E, Chytil, F. Presence of cellular rentinol and retinoic acid binding proteins in experimental rumors. Cancer Lett, 2(1), 25-30, 1976

Ong, D E, Chytil, F. Changes in levels of cellular retinol- and retinoic-acid-binding proteins of liver and lung during perinatal development of rat. Proc Natl Acad Sci U S A, 73(11), 3976-8, 1976 PMCID:431289

Ong, D E, Tsai, C H, Chytil, F. Cellular retinol-binding protein and retinoic acid-binding protein in rat testes: effect of retinol depletion. J Nutr, 106(2), 204-11, 1976

Chytil, F, Page, D L, Ong, D E. Presence of cellular retinol and retinoic acid binding proteins in human uterus. Int J Vitam Nutr Res, 45(3), 293-8, 1975

Ong, D E, Chytil, F. Specificity of cellular retinol-binding protein for compounds with vitamin A activity. Nature, 255(5503), 74-5, 1975

Ong, D E, Chytil, F. Retinoic acid-binding protein in rat tissue. Partial purification and comparison to rat tissue retinol-binding protein. J Biol Chem, 250(15), 6113-7, 1975

Ong, D E, Page, D L, Chytil, F. Retinoic acid binding protein: occurrence in human tumors. Science, 190(4209), 60-1, 1975

Ong, D E, Brady, R N. Isolation of cholinergic receptor protein(s) from Torpedo nobiliana by affinity chromatography. Biochemistry, 13(14), 2822-7, 1974

Ong, D E, Chytil, F. Multiple retinol binding proteins in rabbit lung. Biochem Biophys Res Commun, 59(1), 221-9, 1974

Ong, D E, Brady, R N. In vivo studies on the introduction of the 4-t-double bond of the sphingenine moiety of rat brain ceramides. J Biol Chem, 248(11), 3884-8, 1973

Ong, D E, Brady, R N. Synthesis of ceramides using N-hydroxysuccinimide esters. J Lipid Res, 13(6), 819-22, 1972

Ong, D E, Emery, T F. Ferrichrome biosynthesis: enzyme catalyzed formation of the hydroxamic acid group. Arch Biochem Biophys, 148(1), 77-83, 1972


Postdoctoral Position Available
No

Postdoctoral Position Details
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Updated Date
10/21/2008