Biomedical Research Education & Training
Faculty Member

Singleton, Charles K., Ph.D.
Chair of the Department of Biological Sciences
Professor of Biological Sciences

Lab Url: http://www.cas.vanderbilt.edu/singleton/singletonlab/

Phone Number: 615-322-2008

Email Address: Charles.K.Singleton@vanderbilt.edu

Singleton, Charles's picture
Academic history
B.S., University of Georgia, Athens, GA
Ph.D., Purdue University, West Lafayette, IN
Postdoc, University of Wisconsin, Madison, WI

Office Address   Mailing Address

1210C Biological Sciences Building

1210C Biological Sciences/MRB III VU Station B 351634 37235-1634


Research Keywords
developmental biology, signal transduction, histidine kinases, genetics, ammonia transporters, translational regulation, initiation factor kinases

Research Specialty
Cell-cell and cell-environment signaling during development.

Research Description
We investigate a variety of regulatory phenomena related to various aspects of development in Dictyostelium. This includes studies on sensory histidine kinases, trans-sensor ammonia transporters, and eIF2-alpha kinases in relation to their role in regulating aspects of multicellular development. The common theme is how communication amoung cells and between cells and their environment is accomplished and implemented to allow coordinated morphological development and cell differentiation. Our studies include the mechamisms underlying the regulation of the timing of development, the choice between alternative developmental pathways, the coordination of differentiation and morphogenesis, and the transition from growth to multicellular development. Our main approaches are genetic, molecular genetic, and biochemical.

Publications
Singleton, CK, Xiong, Y, Kirsten, JH, Pendleton, KP. eIF2?? kinases regulate development through the BzpR transcription factor in Dictyostelium discoideum. PLoS One, 7(3), e32500, 2012

Bowman, RL, Xiong, Y, Kirsten, JH, Singleton, CK. eIF2?? kinases control chalone production in Dictyostelium discoideum. Eukaryot Cell, 10(4), 494-501, 2011

Kirsten, JH, Xiong, Y, Davis, CT, Singleton, CK. Subcellular localization of ammonium transporters in Dictyostelium discoideum. BMC Cell Biol, 9, 71, 2008 PMCID:2653498

Rai, M, Xiong, Y, Singleton, CK. Disruption of the ifkA and ifkB genes results in altered cell adhesion, morphological defects and a propensity to form pre-stalk O cells during development of Dictyostelium. Differentiation, 74(9-10), 583-95, 2006

Singleton, CK, Kirsten, JH, Dinsmore, CJ. Function of ammonium transporter A in the initiation of culmination of development in Dictyostelium discoideum. Eukaryot Cell, 5(7), 991-6, 2006 PMCID:1489295

Kirsten, JH, Xiong, Y, Dunbar, AJ, Rai, M, Singleton, CK. Ammonium transporter C of Dictyostelium discoideum is required for correct prestalk gene expression and for regulating the choice between slug migration and culmination. Dev Biol, 287(1), 146-56, 2005

Peter R. Martin, Charles K. Singleton, and Susanne Hiller-Sturmh?fel. The Role of Thiamine Deficiency in Alcoholic Brain Disease. Alcohol Research & Health.. Alcohol Research & Health, 27, 134-142, 2004

Fang, R, Xiong, Y, Singleton, CK. IfkA, a presumptive eIF2 alpha kinase of Dictyostelium, is required for proper timing of aggregation and regulation of mound size. BMC Dev Biol, 3, 3, 2003 PMCID:154100

Follstaedt, SC, Kirsten, JH, Singleton, CK. Temporal and spatial expression of ammonium transporter genes during growth and development of Dictyostelium discoideum. Differentiation, 71(9-10), 557-66, 2003

Martin, PR, Singleton, CK, Hiller-Sturmh??fel, S. The role of thiamine deficiency in alcoholic brain disease. Alcohol Res Health, 27(2), 134-42, 2003

Song, Q, Singleton, CK. Mitochondria from cultured cells derived from normal and thiamine-responsive megaloblastic anemia individuals efficiently import thiamine diphosphate. BMC Biochem, 3, 8, 2002 PMCID:111190

Singleton, C. K. Transketolase. Encyclopedia of Molecular Medicine, 3224-322, 2001

Singleton, CK, Martin, PR. Molecular mechanisms of thiamine utilization. Curr Mol Med, 1(2), 197-207, 2001

Wang, J. J-L., Hua, Z., Fentress, H. M., and Singleton, C. K. JNK1 is inactivated during thiamine deficiency-induced apoptosis in human neruoblastoma cells. J. Nutri. Biochem., 11, 208-215, 2000

Ragan, P.W., Singleton, C.K., and Martin, P.R. Brain injury associated with chronic alcoholism. CNS Spectrums, 4, 66-87, 1999

Pekovich, S. R., Martin, P. R., Poggi, V. and Singleton, C. K. Sensitivity to thiamine deficiency in cultered human cells is dependent on cell type and is enhanced in cells from thiamine- responsive megaloblastic anemia patients. J. Nutri. Biochem, 9, 215-222, 1998

Pekovich, SR, Martin, PR, Singleton, CK. Thiamine deficiency decreases steady-state transketolase and pyruvate dehydrogenase but not alpha-ketoglutarate dehydrogenase mRNA levels in three human cell types. J Nutr, 128(4), 683-7, 1998

Singleton, CK, Zinda, MJ, Mykytka, B, Yang, P. The histidine kinase dhkC regulates the choice between migrating slugs and terminal differentiation in Dictyostelium discoideum. Dev Biol, 203(2), 345-57, 1998

Zinda, MJ, Singleton, CK. The hybrid histidine kinase dhkB regulates spore germination in Dictyostelium discoideum. Dev Biol, 196(2), 171-83, 1998

Singleton, CK. Identification and characterization of the thiamine transporter gene of Saccharomyces cerevisiae. Gene, 199(1-2), 111-21, 1997

Wang, JJ, Martin, PR, Singleton, CK. Aspartate 155 of human transketolase is essential for thiamine diphosphate-magnesium binding, and cofactor binding is required for dimer formation. Biochim Biophys Acta, 1341(2), 165-72, 1997

Wang, JJ, Martin, PR, Singleton, CK. A transketolase assembly defect in a Wernicke-Korsakoff syndrome patient. Alcohol Clin Exp Res, 21(4), 576-80, 1997

Pekovich, SR, Martin, PR, Singleton, CK. Thiamine pyrophosphate-requiring enzymes are altered during pyrithiamine-induced thiamine deficiency in cultured human lymphoblasts. J Nutr, 126(7), 1791-8, 1996

Singleton, CK, Wang, JJ, Shan, L, Martin, PR. Conserved residues are functionally distinct within transketolases of different species. Biochemistry, 35(49), 15865-9, 1996

Wang, J.-L., Martin, P. R., and Singleton, C. K. Aspartate 155 of human transketolase is essential for thiamine pyrophosphate-magnesium binding, and cofactor binding is required for dimer formation. Biochem. et. Biophys. Acta, 1341, 165-172, 1996

Martin, PR, McCool, BA, Singleton, CK. Molecular genetics of transketolase in the pathogenesis of the Wernicke-Korsakoff syndrome. Metab Brain Dis, 10(1), 45-55, 1995

Singleton, CK, Pekovich, SR, McCool, BA, Martin, PR. The thiamine-dependent hysteretic behavior of human transketolase: implications for thiamine deficiency. J Nutr, 125(2), 189-94, 1995

Ken, R, Singleton, CK. Redundant regulatory elements account for the developmental control of a ribosomal protein gene of Dictyostelium discoideum. Differentiation, 55(2), 97-103, 1994

Martin, PR, Pekovich, SR, McCool, BA, Whetsell, WO, Singleton, CK. Thiamine utilization in the pathogenesis of alcohol-induced brain damage. Alcohol Alcohol Suppl, 2, 273-9, 1994

Martin, P.R., McCool, B.A., and Singleton, C.K. Genetic sensitivity to thiamine deficiency and development of alcoholic organic brain disease. Alcoholism: Clin. & Exp. Res., 17, 31-37, 1993

Martin, PR, McCool, BA, Singleton, CK. Genetic sensitivity to thiamine deficiency and development of alcoholic organic brain disease. Alcohol Clin Exp Res, 17(1), 31-7, 1993

McCool, B. A., Plonk, S. G., Martin, P. R., and Singleton, C. K. Cloning of human transketolase and comparison of the nucleotide sequence of the coding region in Wernicke-Korsakoff and non-Wernicke-Korsakoff individuals. J. Biol. Chem., 268, 1397-1404, 1993

McCool, BA, Plonk, SG, Martin, PR, Singleton, CK. Cloning of human transketolase cDNAs and comparison of the nucleotide sequence of the coding region in Wernicke-Korsakoff and non-Wernicke-Korsakoff individuals. J Biol Chem, 268(2), 1397-404, 1993

McPherson, CE, Singleton, CK. Nutrient-responsive promoter elements of the V4 gene of Dictyostelium discoideum. J Mol Biol, 232(2), 386-96, 1993

McPherson, CE, Singleton, CK. V4, a gene required for the transition from growth to development in Dictyostelium discoideum. Dev Biol, 150(2), 231-42, 1992

Singleton, CK, Delude, RL, Ken, R, Manning, SS, McPherson, CE. Structure, expression, and regulation of members of the developmentally controlled V and H gene classes from Dictyostelium. Dev Genet, 12(1-2), 88-97, 1991

Singleton, CK. Nucleotide sequence of V1, a ribosomal protein gene from Dictyostelium discoideum. Nucleic Acids Res, 17(19), 7989, 1989 PMCID:334908

Singleton, CK, Manning, SS, Ken, R. Primary structure and regulation of vegetative specific genes of Dictyostelium discoideum. Nucleic Acids Res, 17(23), 9679-92, 1989 PMCID:335206

Singleton, CK, Gregoli, PA, Manning, SS, Northington, SJ. Characterization of genes which are transiently expressed during the preaggregative phase of development of Dictyostelium discoideum. Dev Biol, 129(1), 140-6, 1988

Singleton, CK, Manning, SS, Feng, Y. Effect of protein synthesis inhibition on gene expression during early development of Dictyostelium discoideum. Mol Cell Biol, 8(1), 10-6, 1988 PMCID:363071

Singleton, CK, McPherson, CE, Manning, SS. Deactivation of gene expression upon the onset of development in dictyostelium discoideum. Dev Genet, 9(4-5), 327-35, 1988

Singleton, C.K. Left-handed helical DNA in solution. Topics in Nucleic Acids Structure 3, (S. Neidle, ed.).Macmillan Publishers LTD, Houndsmill, UK, 163-192, 1987

Singleton, C.K., Delude, R.L., and McPherson, C.E. Characterization of genes which are deactivated upon the onset of development of Dictyostelium discoideum. Dev. Biol., 119, 433-441, 1987

Singleton, CK. pPAV, a promoter analysis vector for Dictyostelium discoideum. Nucleic Acids Res, 15(22), 9610, 1987 PMCID:306502

Singleton, C.K. Structural heterogeneity of duplex DNA. Cell Proliferation: Recent Advances, vol. I (A.L. Boynton and H.L. Leffert, eds.). Academic Press, Inc., New York, NY, 417-447, 1985

Singleton, C.K., Kilpatrick, M.W., and Wells, R.D. S1 nuclease recognizes DNA conformational junctions between left-handed helical (dT-dG)n .(dC-dA)n and contiguous right-handed sequences . J.Biol. Chem., 259, 1963-1967, 1984

Singleton, C.K. Effects of salts, temperature, and stem length on supercoil induced formation of cruciforms. J. Biol. Chem., 258, 7661-7668, 1983

Singleton, C.K., Klysik, J., and Wells, R.D. Conformational flexibility of junctions between contiguous B and Z DNA in supercoiled plasmids. Proc. Natl. Acad. Sci. USA, 80, 2447-2451, 1983

Singleton, C.K. and Wells, R.D. The facile generation of covalently closed, circular DNAs of defined negative superhelical densities. Anal. Biochem., 122, 253-257, 1982

Singleton, C.K., Klysik, J., Stirdivant, S.M., and Wells, R.D. Left-handed Z-DNA is induced by supercoiling under physiological ionic conditions . Nature, 299, 312 316, 1982


Postdoctoral Position Available
No

Postdoctoral Position Details
N/A

Updated Date
05/06/2009