Biomedical Research Education & Training
Faculty Member

Waterman, Michael R., Ph.D.
Professor of Biochemistry, Emeritus

Lab Url: http://medschool.mc.vanderbilt.edu/watermanlab/

Phone Number: 615-322-3318

Email Address: michael.waterman@vanderbilt.edu

Waterman, Michael's picture
Academic history
B.A., Willamette University, Salem, OR
Ph.D., Univ. Orgon Med School, Portland, OR
Post-doc, Univ Pennsylvania School of Medicine

Office Address   Mailing Address

607 Light Hall Vanderbilt University
Nashville, TN 37232-0146

607 Light Hall 0146


Research Keywords
Biochemistry

Research Specialty
Structure, function and regulation of cytochrome P450 enzymes required for lipid biosynthesis and metabolism.

Research Description
Our research focus is on cytochrome P450 (CYP) structure/function. Of particular interest is the P450 required for sterol biosynthesis (CYP51) and the 18 P450 enzymes expressed in the soil bacterium Streptomyces coelicolor.
CYP51
CYP51 (sterol 14a-demethylase) is the most widely expressed member of the CYP (P450) superfamily of genes. It is found in all kingdoms of life, animals, plants, yeast and fungi, lower eukaryotes and a few bacteria including Mycobacterium tuberculosis (MTCYP51). Unlike eukaryotic forms of CYP51, which are membrane bound, the bacterial forms are soluble. Taking advantage of this feature of MTCYP51 we have recently determined the high-resolution crystal structure of MTCYP51 at 2.1 ?.
Sequence alignment of the more than 40 known CYP51 sequences including forms from animals, plants, fungi and bacteria identifies about 40 amino acids that are conserved across more than 400 million years of evolution. We propose that these conserved residues represent the minimal requirement for a sterol 14a-demethylase and have begun a detailed study by mutagenesis in both MTCYP51 and human CYP51 to determine the precise role of each conserved residue in CYP51 structure/function. Combining the biochemical and biophysical properties of these mutants with the known structure of MT CYP51 will permit detailed understanding of why these residues have been conserved over such a long period of evolution
Plant forms of CYP51 have greater substrate specificity than those from other phyla. In fact, certain plant forms (i.e. sorghum) will only use obtusifoliol as substrate. We are using mutagenesis of MTCYP51 and sorghum CYP51 to identify specific residues which influence substrate specificity. Also we will determine the structural basis of azole binding to CYP51. Azole compounds bind directly to the heme iron, inhibiting activity of CYP51 and therefore blocking sterol biosynthesis. Infection by the human pathogen Candida albicans is treated with azole compounds and azole-resistant forms of C. albicans appear which have mutations in the CYP51 gene. We are trying to establish the structural basis for reduced azole binding relative to substrate binding in these mutants
Streptomyces coelicolor P450s
Many bacteria, such as E. coli contain no CYP genes. Others contain one or a very few such genes. However, the actinomycetes Mycobacterium tuberculosis and Streptomyces coelicolor contain 20 and 18 CYP genes, respectively. Being a soil bacterium, Streptomyces has developed two general systems for defense in such a potentially hazardous environment. First, it produces antibiotics which protect it from other organisms which would use Streptomyces as a food source. Strains of Streptomyces produce about 70% of the commercially used antimicrobial compounds for both human and veterinary medicine as well as other important medical compounds such as the antitumor agent, adriamycin. Second, Streptomyces has the capacity to detoxify xenobiotic compounds from its soil environment, thereby protecting itself from mutagens and other poisons. We have begun a long-term project to characterize in detail the structure/function of all 18 CYPs in Streptomyces coelicolor in collaboration with Steve Kelly and David Lamb, University of Wales, Aberystwyth, and Fred Guengerich, Director of the VU Center for Molecular Toxicology. The goals of this project are to develop a rational protocol for design and synthesis of new and potent antibiotics, and to generate modified strains of Streptomyces which can be used for bioremediation of toxic compounds in the environment. The specific aims of this project are: (i) to determine the function of each of the individual S. coelicolor CYPs by disruption of each of the 18 CYP genes and evaluation of resulting phenotypes including ability to kill invading bacteria and identification of changes in profiles of secondary metabolites, (ii) to determine the basis of regulation of CYP expression and activity in S. coelicolor, (iii) to determine the high resolution x-ray structure of substrate-free and substrate-bound forms of the S. coelicolor CYPs, (iv) to establish mutagenesis strategies which will change the activities of the individual CYPs in specific ways, leading to mutant forms of S. coelicolor which will produce novel antibiotics and other medically relevant compounds or will efficiently detoxify mutagens in the environment.

Publications
Gainer, JV, Lipkowitz, MS, Yu, C, Waterman, MR, Dawson, EP, Capdevila, JH, Brown, NJ, , . Association of a CYP4A11 variant and blood pressure in black men. J Am Soc Nephrol, 19(8), 1606-12, 2008 PMCID:2488260

Zhao, B, Lin, X, Lei, L, Lamb, DC, Kelly, SL, Waterman, MR, Cane, DE. Biosynthesis of the sesquiterpene antibiotic albaflavenone in Streptomyces coelicolor A3(2). J Biol Chem, 283(13), 8183-9, 2008 PMCID:2276382

Chun, YJ, Shimada, T, Sanchez-Ponce, R, Martin, MV, Lei, L, Zhao, B, Kelly, SL, Waterman, MR, Lamb, DC, Guengerich, FP. Electron transport pathway for a streptomyces cytochrome P450. Cytochrome P450 105D5-catalyzed fatty acid hydroxylation in streptomyces coelicolor A3(2). J Biol Chem, 2007

Lepesheva, GI, Ott, RD, Hargrove, TY, Kleshchenko, YY, Schuster, I, Nes, WD, Hill, GC, Villalta, F, Waterman, MR. Sterol 14alpha-demethylase as a potential target for antitrypanosomal therapy: enzyme inhibition and parasite cell growth. Chem Biol, 14(11), 1283-93, 2007 PMCID:2324070

Lepesheva, GI, Seliskar, M, Knutson, CG, Stourman, NV, Rozman, D, Waterman, MR. Conformational dynamics in the F/G segment of CYP51 from Mycobacterium tuberculosis monitored by FRET. Arch Biochem Biophys, 464(2), 221-7, 2007

Lepesheva, GI, Waterman, MR. Sterol 14alpha-demethylase cytochrome P450 (CYP51), a P450 in all biological kingdoms. Biochim Biophys Acta, 1770(3), 467-77, 2007 PMCID:2324071

Zhao, B, Lamb, DC, Lei, L, Kelly, SL, Yuan, H, Hachey, DL, Waterman, MR. Different binding modes of two flaviolin substrate molecules in cytochrome P450 158A1 (CYP158A1) compared to CYP158A2. Biochemistry, 46(30), 8725-33, 2007

Zhao, B, Waterman, MR. Novel properties of P450s in Streptomyces coelicolor. Drug Metab Rev, 39(2-3), 343-52, 2007

Arase, M, Waterman, MR, Kagawa, N. Purification and characterization of bovine steroid 21-hydroxylase (P450c21) efficiently expressed in Escherichia coli. Biochem Biophys Res Commun, 344(1), 400-5, 2006

Chun, YJ, Shimada, T, Waterman, MR, Guengerich, FP. Understanding electron transport systems of Streptomyces cytochrome P450. Biochem Soc Trans, 34(Pt 6), 1183-5, 2006

Lamb, DC, Guengerich, FP, Kelly, SL, Waterman, MR. Exploiting Streptomyces coelicolor A3(2) P450s as a model for application in drug discovery. Expert Opin Drug Metab Toxicol, 2(1), 27-40, 2006

Lepesheva, GI, Hargrove, TY, Ott, RD, Nes, WD, Waterman, MR. Biodiversity of CYP51 in trypanosomes. Biochem Soc Trans, 34(Pt 6), 1161-4, 2006

Lepesheva, GI, Zaitseva, NG, Nes, WD, Zhou, W, Arase, M, Liu, J, Hill, GC, Waterman, MR. CYP51 from Trypanosoma cruzi: a phyla-specific residue in the B'' helix defines substrate preferences of sterol 14alpha-demethylase. J Biol Chem, 281(6), 3577-85, 2006

Nakagawa, K, Holla, VR, Wei, Y, Wang, WH, Gatica, A, Wei, S, Mei, S, Miller, CM, Cha, DR, Price, E, Zent, R, Pozzi, A, Breyer, MD, Guan, Y, Falck, JR, Waterman, MR, Capdevila, JH. Salt-sensitive hypertension is associated with dysfunctional Cyp4a10 gene and kidney epithelial sodium channel. J Clin Invest, 116(6), 1696-702, 2006 PMCID:1459070

Zhao, B, Waterman, MR, Isin, EM, Sundaramoorthy, M, Podust, LM. Ligand-assisted inhibition in cytochrome P450 158A2 from Streptomyces coelicolor A3(2). Biochemistry, 45(24), 7493-500, 2006

Zhou, W, Lepesheva, GI, Waterman, MR, Nes, WD. Mechanistic Analysis of a Multiple Product Sterol Methyltransferase Implicated in Ergosterol Biosynthesis in Trypanosoma brucei. J Biol Chem, 281(10), 6290-6, 2006

Gainer, JV, Bellamine, A, Dawson, EP, Womble, KE, Grant, SW, Wang, Y, Cupples, LA, Guo, CY, Demissie, S, O'Donnell, CJ, Brown, NJ, Waterman, MR, Capdevila, JH. Functional variant of CYP4A11 20-hydroxyeicosatetraenoic acid synthase is associated with essential hypertension. Circulation, 111(1), 63-9, 2005

Kagawa, N, Senbonmatsu, TA, Satoh, K, Ichihara, K, Yamagata, N, Hatano, O, Saito, T, Nguyen, VQ, Waterman, MR, Price, E, Atkinson, JB, Inagami, T. Tetracycline protects myocardium against ischemic injury. Front Biosci, 10, 608-19, 2005

Waterman, MR. Professor Howard Mason and oxygen activation. Biochem Biophys Res Commun, 338(1), 7-11, 2005

Waterman, MR, Lepesheva, GI. Sterol 14 alpha-demethylase, an abundant and essential mixed-function oxidase. Biochem Biophys Res Commun, 338(1), 418-22, 2005

Zhao, B, Guengerich, FP, Voehler, M, Waterman, MR. Role of active site water molecules and substrate hydroxyl groups in oxygen activation by cytochrome P450 158A2: a new mechanism of proton transfer. J Biol Chem, 280(51), 42188-97, 2005

Zhao, B, Peter Guengerich, F, Bellamine, A, Lamb, DC, Izumikawa, M, Lei, L, Podust, LM, Sundaramoorthy, M, Kalaitzis, JA, Manmohan Reddy, L, Kelly, SL, Moore, BS, Stec, D, Voehler, M, Falck, JR, Shimada, T, Waterman, MR. Binding of two flaviolin substrate molecules, oxidative coupling, and crystal structure of streptomyces coelicolor A3(2) cytochrome P450 158A2. J Biol Chem, 2005

Bellamine, A, Lepesheva, GI, Waterman, MR. Fluconazole binding and sterol demethylation in three CYP51 isoforms indicate differences in active site topology. J Lipid Res, 45(11), 2000-7, 2004

Kagawa, N, Hori, H, Waterman, MR, Yoshioka, S. Characterization of stable human aromatase expressed in E. coli. Steroids, 69(4), 235-43, 2004

Lepesheva, GI, Nes, WD, Zhou, W, Hill, GC, Waterman, MR. CYP51 from Trypanosoma brucei is obtusifoliol-specific. Biochemistry, 43(33), 10789-99, 2004

Lepesheva, Galina I, Waterman, Michael R. CYP51-the omnipotent P450. Mol Cell Endocrinol, 215(1-2), 165-70, 2004

Podust, LM, Yermalitskaya, LV, Lepesheva, GI, Podust, VN, Dalmasso, EA, Waterman, MR. Estriol bound and ligand-free structures of sterol 14alpha-demethylase. Structure (Camb), 12(11), 1937-45, 2004

Podust, Larissa M, Bach, Horacio, Kim, Youngchang, Lamb, David C, Arase, Miharu, Sherman, David H, Kelly, Steven L, Waterman, Michael R. Comparison of the 1.85 A structure of CYP154A1 from Streptomyces coelicolor A3(2) with the closely related CYP154C1 and CYPs from antibiotic biosynthetic pathways. Protein Sci, 13(1), 255-68, 2004 PMCID:2286509

Bellamine, Aouatef, Wang, Yarong, Waterman, Michael R, Gainer, James V, Dawson, Elliot P, Brown, Nancy J, Capdevila, Jorge H. Characterization of the CYP4A11 gene, a second CYP4A gene in humans. Arch Biochem Biophys, 409(1), 221-7, 2003

Fon Tacer, Klementina, Kalanj-Bognar, Svjetlana, Waterman, Michael R, Rozman, Damjana, . Lanosterol metabolism and sterol regulatory element binding protein (SREBP) expression in male germ cell maturation.. J Steroid Biochem Mol Biol, 85, 429-38, 2003

Lamb, David C, Ikeda, Haruo, Nelson, David R, Ishikawa, Jun, Skaug, Tove, Jackson, Colin, Omura, Satoshi, Waterman, Michael R, Kelly, Steven L. Cytochrome p450 complement (CYPome) of the avermectin-producer Streptomyces avermitilis and comparison to that of Streptomyces coelicolor A3(2). Biochem Biophys Res Commun, 307(3), 610-9, 2003

Lepesheva, Galina I, Virus, Cornelia, Waterman, Michael R. Conservation in the CYP51 Family. Role of the B' Helix/BC Loop and Helices F and G in Enzymatic Function. Biochemistry, 42(30), 9091-101, 2003

Nakagawa, Kiyoshi, Marji, Jackleen S, Schwartzman, Michal L, Waterman, Michael R, Capdevila, Jorge H. Androgen-mediated induction of the kidney arachidonate hydroxylases is associated with the development of hypertension. Am J Physiol Regul Integr Comp Physiol, 284(4), R1055-62, 2003

Podust, Larissa M, Kim, Youngchang, Arase, Miharu, Neely, Benjamin A, Beck, Brian J, Bach, Horacio, Sherman, David H, Lamb, David C, Kelly, Steven L, Waterman, Michael R. The 1.92-A structure of Streptomyces coelicolor A3(2) CYP154C1. A new monooxygenase that functionalizes macrolide ring systems. J Biol Chem, 278(14), 12214-21, 2003

Sewer, Marion B, Waterman, Michael R. CAMP-dependent protein kinase enhances CYP17 transcription via MKP-1 activation in H295R human adrenocortical cells. J Biol Chem, 278(10), 8106-11, 2003

Sewer, Marion B, Waterman, Michael R. ACTH modulation of transcription factors responsible for steroid hydroxylase gene expression in the adrenal cortex. Microsc Res Tech, 61(3), 300-7, 2003

Halder, Sunil K, Fink, Martina, Waterman, Michael R, Rozman, Damjana. A cAMP-responsive element binding site is essential for sterol regulation of the human lanosterol 14alpha-demethylase gene (CYP51). Mol Endocrinol, 16, 1853-63, 2002

Lamb, David C, Fowler, Kay, Kieser, Tobias, Manning, Nigel, Podust, Larissa M, Waterman, Michael R, Kelly, Diane E, Kelly, Steven L. Sterol 14alpha-demethylase activity in Streptomyces coelicolor A3(2) is associated with an unusual member of the CYP51 gene family. Biochem J, 364, 555-62, 2002 PMCID:1222601

Lamb, David C, Skaug, Tove, Song, Hong-Lin, Jackson, Colin J, Podust, Larissa M, Waterman, Michael R, Kell, Douglas B, Kelly, Diane E, Kelly, Steven L. The cytochrome P450 complement (CYPome) of Streptomyces coelicolor A3(2). J Biol Chem, 277, 24000-5, 2002

Sewer, M B, Waterman, M R. Transcriptional complexes at the CYP17 CRS. Endocr Res, 28, 551-8, 2002

Sewer, M B, Waterman, M R. cAMP-dependent transcription of steroidogenic genes in the human adrenal cortex requires a dual-specificity phosphatase in addition to protein kinase A. J Mol Endocrinol, 29, 163-74, 2002

Sewer, Marion B, Nguyen, Viet Q, Huang, Ching-Jung, Tucker, Philip W, Kagawa, Norio, Waterman, Michael R. Transcriptional activation of human CYP17 in H295R adrenocortical cells depends on complex formation among p54(nrb)/NonO, protein-associated splicing factor, and SF-1, a complex that also participates in repression of transcription. Endocrinology, 143, 1280-90, 2002

Sewer, Marion B, Waterman, Michael R. Adrenocorticotropin/cyclic adenosine 3',5'-monophosphate-mediated transcription of the human CYP17 gene in the adrenal cortex is dependent on phosphatase activity. Endocrinology, 143, 1769-77, 2002

Tom, Moshe, Myers, C R, Waterman, Michael R. Evaluating molar CYP1A level in fish hepatic microsomes by competitive ELISA using recombinant membrane-free CYP1A standard protein. Aquat Toxicol, 59, 101-14, 2002

Anandatheerthavarada, H K, Amuthan, G, Biswas, G, Robin, M A, Murali, R, Waterman, M R, Avadhani, N G. Evolutionarily divergent electron donor proteins interact with P450MT2 through the same helical domain but different contact points. EMBO J, 20, 2394-403, 2001 PMCID:125462

Bellamine, A, Mangla, A T, Dennis, A L, Nes, W D, Waterman, M R. Structural requirements for substrate recognition of Mycobacterium tuberculosis 14 alpha-demethylase: implications for sterol biosynthesis. J Lipid Res, 42, 128-36, 2001

Holla, V R, Adas, F, Imig, J D, Zhao, X, Price, E, Olsen, N, Kovacs, W J, Magnuson, M A, Keeney, D S, Breyer, M D, Falck, J R, Waterman, M R, Capdevila, J H. Alterations in the regulation of androgen-sensitive Cyp 4a monooxygenases cause hypertension. Proc Natl Acad Sci U S A, 98, 5211-6, 2001 PMCID:33189

Kusano, K, Kagawa, N, Sakaguchi, M, Omura, T, Waterman, M R. Importance of a proline-rich sequence in the amino-terminal region for correct folding of mitochondrial and soluble microbial p450s. J Biochem (Tokyo), 129, 271-7, 2001

Kusano, K, Sakaguchi, M, Kagawa, N, Waterman, M R, Omura, T. Microsomal p450s use specific proline-rich sequences for efficient folding, but not for maintenance of the folded structure. J Biochem (Tokyo), 129, 259-69, 2001

Lepesheva, G I, Podust, L M, Bellamine, A, Waterman, M R. Folding requirements are different between sterol 14alpha-demethylase (CYP51) from Mycobacterium tuberculosis and human or fungal orthologs. J Biol Chem, 276, 28413-20, 2001

Podust, L M, Poulos, T L, Waterman, M R. Crystal structure of cytochrome P450 14alpha -sterol demethylase (CYP51) from Mycobacterium tuberculosis in complex with azole inhibitors. Proc Natl Acad Sci U S A, 98, 3068-73, 2001 PMCID:30608

Podust, L M, Stojan, J, Poulos, T L, Waterman, M R. Substrate recognition sites in 14alpha-sterol demethylase from comparative analysis of amino acid sequences and X-ray structure of Mycobacterium tuberculosis CYP51. J Inorg Biochem, 87, 227-35, 2001

Sewer, M B, Waterman, M R. Insights into the transcriptional regulation of steroidogenic enzymes and StAR. Rev Endocr Metab Disord, 2, 269-74, 2001

Brock, B J, Waterman, M R. The use of random chimeragenesis to study structure/function properties of rat and human P450c17. Arch Biochem Biophys, 373, 401-8, 2000

Cheng, P Y, Kagawa, N, Takahashi, Y, Waterman, M R. Three zinc finger nuclear proteins, Sp1, Sp3, and a ZBP-89 homologue, bind to the cyclic adenosine monophosphate-responsive sequence of the bovine adrenodoxin gene and regulate transcription. Biochemistry, 39, 4347-57, 2000

Majdic, G, Parvinen, M, Bellamine, A, Harwood , H J, Ku, W W, Waterman, M R, Rozman, D. Lanosterol 14alpha-demethylase (CYP51), NADPH-cytochrome P450 reductase and squalene synthase in spermatogenesis: late spermatids of the rat express proteins needed to synthesize follicular fluid meiosis activating sterol. J Endocrinol, 166, 463-74, 2000

Pikuleva, I A, Tesh, K, Waterman, M R, Kim, Y. The tertiary structure of full-length bovine adrenodoxin suggests functional dimers. Arch Biochem Biophys, 373, 44-55, 2000

Ahlgren, R, Suske, G, Waterman, M R, Lund, J. Role of Sp1 in cAMP-dependent transcriptional regulation of the bovine CYP11A gene. J Biol Chem, 274, 19422-8, 1999

Bellamine, A, Mangla, A T, Nes, W D, Waterman, M R. Characterization and catalytic properties of the sterol 14alpha-demethylase from Mycobacterium tuberculosis. Proc Natl Acad Sci U S A, 96, 8937-42, 1999 PMCID:17711

Brock, B J, Waterman, M R. Biochemical differences between rat and human cytochrome P450c17 support the different steroidogenic needs of these two species. Biochemistry, 38, 1598-606, 1999

Jenkins, C M, Waterman, M R. Flavodoxin as a model for the P450-interacting domain of NADPH cytochrome P450 reductase. Drug Metab Rev, 31, 195-203, 1999

Kagawa, N, Bischof, L J, Cheng, P Y, Anwar, A, Waterman, M R. Biochemical diversity of peptide-hormone-dependent regulation of steroidogenic P450s. Drug Metab Rev, 31, 333-42, 1999

Keeney, D S, Waterman, M R. Two novel sites of expression of NADPH cytochrome P450 reductase during murine embryogenesis: limb mesenchyme and developing olfactory neuroepithelia. Dev Dyn, 216, 511-7, 1999

Kusano, K, Waterman, M R, Sakaguchi, M, Omura, T, Kagawa, N. Protein synthesis inhibitors and ethanol selectively enhance heterologous expression of P450s and related proteins in Escherichia coli. Arch Biochem Biophys, 367, 129-36, 1999

Lamb, D C, Kelly, D E, Waterman, M R, Stromstedt, M, Rozman, D, Kelly, S L. Characteristics of the heterologously expressed human lanosterol 14alpha-demethylase (other names: P45014DM, CYP51, P45051) and inhibition of the purified human and Candida albicans CYP51 with azole antifungal agents. Yeast, 15, 755-63, 1999

Pikuleva, I A, Cao, C, Waterman, M R. An additional electrostatic interaction between adrenodoxin and P450c27 (CYP27A1) results in tighter binding than between adrenodoxin and p450scc (CYP11A1). J Biol Chem, 274, 2045-52, 1999

Rozman, D, Fink, M, Fimia, G M, Sassone-Corsi, P, Waterman, M R. Cyclic adenosine 3',5'-monophosphate(cAMP)/cAMP-responsive element modulator (CREM)-dependent regulation of cholesterogenic lanosterol 14alpha-demethylase (CYP51) in spermatids. Mol Endocrinol, 13, 1951-62, 1999

Tom, M, Waterman, M R, Bocking, D, Dauphin-Villemant, C. Cloning and characterization of the L15 ribosomal protein gene homologue from the crayfish Orconectes limosus. Biochem Biophys Res Commun, 256, 313-6, 1999

Bischof, L J, Kagawa, N, Moskow, J J, Takahashi, Y, Iwamatsu, A, Buchberg, A M, Waterman, M R. Members of the meis1 and pbx homeodomain protein families cooperatively bind a cAMP-responsive sequence (CRS1) from bovine CYP17. J Biol Chem, 273, 7941-8, 1998

Bischof, L J, Kagawa, N, Waterman, M R. The bovine CYP17 promoter contains a transcriptional regulatory element cooperatively bound by tale homeodomain proteins. Endocr Res, 24, 489-95, 1998

Jenkins, C M, Pikuleva, I, Waterman, M R. Expression of eukaryotic cytochromes P450 in E. coli. Methods Mol Biol, 107, 181-93, 1998

Jenkins, C M, Waterman, M R. NADPH-flavodoxin reductase and flavodoxin from Escherichia coli: characteristics as a soluble microsomal P450 reductase. Biochemistry, 37, 6106-13, 1998

Keeney, D S, Skinner, C, Travers, J B, Capdevila, J H, Nanney, L B, King, L E, Waterman, M R. Differentiating keratinocytes express a novel cytochrome P450 enzyme, CYP2B19, having arachidonate monooxygenase activity. J Biol Chem, 273, 32071-9, 1998

Keeney, D S, Skinner, C, Wei, S, Friedberg, T, Waterman, M R. A keratinocyte-specific epoxygenase, CYP2B12, metabolizes arachidonic acid with unusual selectivity, producing a single major epoxyeicosatrienoic acid. J Biol Chem, 273, 9279-84, 1998

Nonaka, Y, Fujii, T, Kagawa, N, Waterman, M R, Takemori, H, Okamoto, M. Structure/function relationship of CYP11B1 associated with Dahl's salt-resistant rats--expression of rat CYP11B1 and CYP11B2 in Escherichia coli. Eur J Biochem, 258, 869-78, 1998

Pikuleva, I A, Babiker, A, Waterman, M R, Bjorkhem, I. Activities of recombinant human cytochrome P450c27 (CYP27) which produce intermediates of alternative bile acid biosynthetic pathways. J Biol Chem, 273, 18153-60, 1998

Rozman, D, Waterman, M R. Lanosterol 14alpha-demethylase (CYP51) and spermatogenesis. Drug Metab Dispos, 26, 1199-201, 1998

Stromstedt, M, Waterman, M R, Haugen, T B, Tasken K, Parvinen, M, Rozman, D. Elevated expression of lanosterol 14alpha-demethylase (CYP51) and the synthesis of oocyte meiosis-activating sterols in postmeiotic germ cells of male rats. Endocrinology, 139, 2314-21, 1998

Waterman, M R. Steroidogenesis, StAR and PBR: is there light at the end of the tunnel?. Proc Soc Exp Biol Med, 217, 121-2, 1998

Bassett, M H, McCarthy, J L, Waterman, M R, Sliter, T J. Sequence and developmental expression of Cyp18, a member of a new cytochrome P450 family from Drosophila. Mol Cell Endocrinol, 131, 39-49, 1997

Jenkins, C M, Genzor, C G, Fillat, M F, Waterman, M R, Gomez-Moreno, C. Negatively charged anabaena flavodoxin residues (Asp144 and Glu145) are important for reconstitution of cytochrome P450 17alpha-hydroxylase activity. J Biol Chem, 272, 22509-13, 1997

Jenkins, C M, Pikuleva, I, Kagawa, N, Waterman, M R. Escherichia coli flavodoxin sepharose as an affinity resin for cytochromes P450 and use to identify a putative cytochrome P450c17/3beta-hydroxysteroid dehydrogenase interaction. Arch Biochem Biophys, 347, 93-102, 1997

Ogo, A, Waterman, M R, Kagawa, N. cAMP-dependent transactivation involving the homeodomain protein Pbx1. Arch Biochem Biophys, 338, 193-200, 1997

Ogo, A, Waterman, M R, McAllister, J M, Kagawa, N. The homeodomain protein Pbx1 is involved in cAMP-dependent transcription of human CYP17. Arch Biochem Biophys, 348, 226-31, 1997

Pikuleva, I A, Bjorkhem, I, Waterman, M R. Expression, purification, and enzymatic properties of recombinant human cytochrome P450c27 (CYP27). Arch Biochem Biophys, 343, 123-30, 1997

Vianello, S, Waterman, M R, Dalla Valle, L, Colombo, L. Developmentally regulated expression and activity of 17alpha-hydroxylase/C-17,20-lyase cytochrome P450 in rat liver. Endocrinology, 138, 3166-74, 1997

Waterman, M R, Bischof, L J. Cytochromes P450 12: diversity of ACTH (cAMP)-dependent transcription of bovine steroid hydroxylase genes. FASEB J, 11, 419-27, 1997

Estabrook, R W, Shet, M S, Fisher, C W, Jenkins, C M, Waterman, M R. The interaction of NADPH-P450 reductase with P450: an electrochemical study of the role of the flavin mononucleotide-binding domain. Arch Biochem Biophys, 333, 308-15, 1996

Nelson, D R, Koymans, L, Kamataki, T, Stegeman, J J, Feyereisen, R, Waxman, D J, Waterman, M R, Gotoh, O, Coon, M J, Estabrook, R W, Gunsalus, I C, Nebert, D W. P450 superfamily: update on new sequences, gene mapping, accession numbers and nomenclature. Pharmacogenetics, 6, 1-42, 1996

Pikuleva, I A, Bjorkhem, I, Waterman, M R. Studies of distant members of the P450 superfamily (P450scc and P450c27) by random chimeragenesis. Arch Biochem Biophys, 334, 183-92, 1996

Rozman, D, Stromstedt, M, Tsui, L C, Scherer, S W, Waterman, M R. Structure and mapping of the human lanosterol 14alpha-demethylase gene (CYP51) encoding the cytochrome P450 involved in cholesterol biosynthesis; comparison of exon/intron organization with other mammalian and fungal CYP genes. Genomics, 38, 371-81, 1996

Rozman, D, Stromstedt, M, Waterman, M R. The three human cytochrome P450 lanosterol 14 alpha-demethylase (CYP51) genes reside on chromosomes 3, 7, and 13: structure of the two retrotransposed pseudogenes, association with a line-1 element, and evolution of the human CYP51 family. Arch Biochem Biophys, 333, 466-74, 1996

Stromstedt, M, Keeney, D S, Waterman, M R, Paria, B C, Conley, A J, Dey, S K. Preimplantation mouse blastocysts fail to express CYP genes required for estrogen biosynthesis. Mol Reprod Dev, 43, 428-36, 1996

Stromstedt, M, Rozman, D, Waterman, M R. The ubiquitously expressed human CYP51 encodes lanosterol 14 alpha-demethylase, a cytochrome P450 whose expression is regulated by oxysterols. Arch Biochem Biophys, 329, 73-81, 1996

Waterman, M R. Introduction: transcription and regulation of activities of cytochromes P450 metabolizing endogenous substrates. FASEB J, 10, 1455, 1996

Waterman, M R, Bischof, L J. Mechanisms of ACTH(cAMP)-dependent transcription of adrenal steroid hydroxylases. Endocr Res, 22, 615-20, 1996

Waterman, M R, Keeney, D S. Signal transduction pathways combining peptide hormones and steroidogenesis. Vitam Horm, 52, 129-48, 1996

Ehrhart-Bornstein, M, Bornstein, S R, Gonzalez-Hernandez, J, Holst, J J, Waterman, M R, Scherbaum, W A. Sympathoadrenal regulation of adrenocortical steroidogenesis. Endocr Res, 21, 13-24, 1995

Katagiri, M, Kagawa, N, Waterman, M R. The role of cytochrome b5 in the biosynthesis of androgens by human P450c17. Arch Biochem Biophys, 317, 343-7, 1995

Keeney, D S, Ikeda, Y, Waterman, M R, Parker, K L. Cholesterol side-chain cleavage cytochrome P450 gene expression in the primitive gut of the mouse embryo does not require steroidogenic factor 1. Mol Endocrinol, 9, 1091-8, 1995

Keeney, D S, Jenkins, C M, Waterman, M R. Developmentally regulated expression of adrenal 17 alpha-hydroxylase cytochrome P450 in the mouse embryo. Endocrinology, 136, 4872-9, 1995

Ogo, A, Waterman, M R, Kamps, M P, Kagawa, N. Protein kinase A-dependent transactivation by the E2A-Pbx1 fusion protein. J Biol Chem, 270, 25340-3, 1995

Pikuleva, I A, Mackman, R L, Kagawa, N, Waterman, M R, Ortiz de Montellano, P R. Active-site topology of bovine cholesterol side-chain cleavage cytochrome P450 (P450scc) and evidence for interaction of tyrosine 94 with the side chain of cholesterol. Arch Biochem Biophys, 322, 189-97, 1995

Stromstedt, M, Waterman, M R. A full-length cDNA encoding mouse adrenodoxin. Biochim Biophys Acta, 1261, 126-8, 1995

Stromstedt, M, Waterman, M R. Messenger RNAs encoding steroidogenic enzymes are expressed in rodent brain. Brain Res Mol Brain Res, 34, 75-88, 1995

Venepally, P, Waterman, M R. Two Sp1-binding sites mediate cAMP-induced transcription of the bovine CYP11A gene through the protein kinase A signaling pathway. J Biol Chem, 270, 25402-10, 1995

Waterman, M R. A rising StAR: an essential role in cholesterol transport. Science, 267, 1780-1, 1995

Waterman, M R, Jenkins, C M, Pikuleva, I. Genetically engineered bacterial cells and applications. Toxicol Lett, 82-83, 807-13, 1995

Barnes, H J, Jenkins, C M, Waterman, M R. Baculovirus expression of bovine cytochrome P450c17 in Sf9 cells and comparison with expression in yeast, mammalian cells, and E. coli. Arch Biochem Biophys, 315(2), 489-94, 1994

Hatano, O, Takayama, K, Imai, T, Waterman, M R, Takakusu, A, Omura, T, Morohashi, K. Sex-dependent expression of a transcription factor, Ad4BP, regulating steroidogenic P-450 genes in the gonads during prenatal and postnatal rat development. Development, 120(10), 2787-97, 1994

Jenkins, C M, Waterman, M R. Flavodoxin and NADPH-flavodoxin reductase from Escherichia coli support bovine cytochrome P450c17 hydroxylase activities. J Biol Chem, 269(44), 27401-8, 1994

Kagawa, N, Ogo, A, Takahashi, Y, Iwamatsu, A, Waterman, M R. A cAMP-regulatory sequence (CRS1) of CYP17 is a cellular target for the homeodomain protein Pbx1. J Biol Chem, 269(29), 18716-9, 1994

Sakai, Y, Yanase, T, Okabe, Y, Hara, T, Waterman, M R, Takayanagi, R, Haji, M, Nawata, H. No mutation in cytochrome P450 side chain cleavage in a patient with congenital lipoid adrenal hyperplasia. J Clin Endocrinol Metab, 79(4), 1198-201, 1994

Waterman, M R. Biochemical diversity of cAMP-dependent transcription of steroid hydroxylase genes in the adrenal cortex. J Biol Chem, 269(45), 27783-6, 1994

Waterman, M R. Heterologous expression of mammalian P450 enzymes. Adv Enzymol Relat Areas Mol Biol, 68, 37-66, 1994

Fevold, H R, Ivanovitch, J D, Zanger, U M, Waterman, M R. The sequence of the 5'-end of the rat CYP17 gene, the transcription initiation site and a comparison with the homologous genes of other species. Mol Cell Endocrinol, 95(1-2), 95-100, 1993

Imai, T, Globerman, H, Gertner, J M, Kagawa, N, Waterman, M R. Expression and purification of functional human 17 alpha-hydroxylase/17,20-lyase (P450c17) in Escherichia coli. Use of this system for study of a novel form of combined 17 alpha-hydroxylase/17,20-lyase deficiency. J Biol Chem, 268(26), 19681-9, 1993

Keeney, D S, Waterman, M R. Regulation of steroid hydroxylase gene expression: importance to physiology and disease. Pharmacol Ther, 58(3), 301-17, 1993

Lauber, M E, Kagawa, N, Waterman, M R, Simpson, E R. cAMP-dependent and tissue-specific expression of genes encoding steroidogenic enzymes in bovine luteal and granulosa cells in primary culture. Mol Cell Endocrinol, 93(2), 227-33, 1993

Lauber, M E, Picton, H M, Begeot, M, Momoi, K, Waterman, M R, Simpson, E R. Regulation of CYP11A gene expression in bovine ovarian granulosa cells in primary culture by cAMP and phorbol esters is conferred by a common cis-acting element. Mol Cell Endocrinol, 94(2), 235-42, 1993

Morohashi, K, Zanger, U M, Honda, S, Hara, M, Waterman, M R, Omura, T. Activation of CYP11A and CYP11B gene promoters by the steroidogenic cell-specific transcription factor, Ad4BP. Mol Endocrinol, 7(9), 1196-204, 1993

Richardson, T H, Hsu, M H, Kronbach, T, Barnes, H J, Chan, G, Waterman, M R, Kemper, B, Johnson, E F. Purification and characterization of recombinant-expressed cytochrome P450 2C3 from Escherichia coli: 2C3 encodes the 6 beta-hydroxylase deficient form of P450 3b. Arch Biochem Biophys, 300(1), 510-6, 1993

Sagara, Y, Barnes, H J, Waterman, M R. Expression in Escherichia coli of functional cytochrome P450c17 lacking its hydrophobic amino-terminal signal anchor. Arch Biochem Biophys, 304(1), 272-8, 1993

Sagara, Y, Wada, A, Takata, Y, Waterman, M R, Sekimizu, K, Horiuchi, T. Direct expression of adrenodoxin reductase in Escherichia coli and the functional characterization. Biol Pharm Bull, 16(7), 627-30, 1993

Swart, P, Swart, A C, Waterman, M R, Estabrook, R W, Mason, J I. Progesterone 16 alpha-hydroxylase activity is catalyzed by human cytochrome P450 17 alpha-hydroxylase. J Clin Endocrinol Metab, 77(1), 98-102, 1993

Trzeciak, W H, LeHoux, J G, Waterman, M R, Simpson, E R. Dexamethasone inhibits corticotropin-induced accumulation of CYP11A and CYP17 messenger RNAs in bovine adrenocortical cells. Mol Endocrinol, 7(2), 206-13, 1993

Waterman, M R. Heterologous expression of cytochrome P-450 in Escherichia coli. Biochem Soc Trans, 21(4), 1081-5, 1993

Ahlgren, R, Yanase, T, Simpson, E R, Winter, J S, Waterman, M R. Compound heterozygous mutations (Arg 239----stop, Pro 342----Thr) in the CYP17 (P45017 alpha) gene lead to ambiguous external genitalia in a male patient with partial combined 17 alpha-hydroxylase/17,20-lyase deficiency. J Clin Endocrinol Metab, 74(3), 667-72, 1992

Chen, J Y, Waterman, M R. Two promoters in the bovine adrenodoxin gene and the role of associated, unique cAMP-responsive sequences. Biochemistry, 31(8), 2400-7, 1992

Clark, B J, Waterman, M R. Functional expression of bovine 17 alpha-hydroxylase in COS 1 cells is dependent upon the presence of an amino-terminal signal anchor sequence. J Biol Chem, 267(34), 24568-74, 1992

Durkee, T J, McLean, M P, Hales, D B, Payne, A H, Waterman, M R, Khan, I, Gibori, G. P450(17 alpha) and P450SCC gene expression and regulation in the rat placenta. Endocrinology, 130(3), 1309-17, 1992

Fan, Y S, Sasi, R, Lee, C, Winter, J S, Waterman, M R, Lin, C C. Localization of the human CYP17 gene (cytochrome P450(17 alpha)) to 10q24.3 by fluorescence in situ hybridization and simultaneous chromosome banding. Genomics, 14(4), 1110-1, 1992

Fisher, C W, Caudle, D L, Martin-Wixtrom, C, Quattrochi, L C, Tukey, R H, Waterman, M R, Estabrook, R W. High-level expression of functional human cytochrome P450 1A2 in Escherichia coli. FASEB J, 6(2), 759-64, 1992

G??se-Behling, H, Ehrhart-Bornstein, M, Bornstein, S R, Waterman, M R, Scherbaum, W A, Adler, G. Regulation of adrenal steroidogenesis by adrenaline: expression of cytochrome P450 genes. J Endocrinol, 135(2), 229-37, 1992

Imai, T, Yanase, T, Waterman, M R, Simpson, E R, Pratt, J J. Canadian Mennonites and individuals residing in the Friesland region of The Netherlands share the same molecular basis of 17 alpha-hydroxylase deficiency. Hum Genet, 89(1), 95-6, 1992

Kagawa, N, Waterman, M R. Purification and characterization of a transcription factor which appears to regulate cAMP responsiveness of the human CYP21B gene. J Biol Chem, 267(35), 25213-9, 1992

Momoi, K, Waterman, M R, Simpson, E R, Zanger, U M. 3',5'-cyclic adenosine monophosphate-dependent transcription of the CYP11A (cholesterol side chain cleavage cytochrome P450) gene involves a DNA response element containing a putative binding site for transcription factor Sp1. Mol Endocrinol, 6(10), 1682-90, 1992

Palin, M F, Berthiaume, L, Lehoux, J G, Waterman, M R, Sygusch, J. Direct expression of mature bovine adrenodoxin in Escherichia coli. Arch Biochem Biophys, 295(1), 126-31, 1992

Wada, A, Waterman, M R. Identification by site-directed mutagenesis of two lysine residues in cholesterol side chain cleavage cytochrome P450 that are essential for adrenodoxin binding. J Biol Chem, 267(32), 22877-82, 1992

Waterman, M R, Keeney, D S. Genes involved in androgen biosynthesis and the male phenotype. Horm Res, 38(5-6), 217-21, 1992

Yanase, T, Waterman, M R, Zachmann, M, Winter, J S, Simpson, E R, Kagimoto, M. Molecular basis of apparent isolated 17,20-lyase deficiency: compound heterozygous mutations in the C-terminal region (Arg(496)----Cys, Gln(461)----Stop) actually cause combined 17 alpha-hydroxylase/17,20-lyase deficiency. Biochim Biophys Acta, 1139(4), 275-9, 1992

Zanger, U M, Kagawa, N, Lund, J, Waterman, M R. Distinct biochemical mechanisms for cAMP-dependent transcription of CYP17 and CYP21. FASEB J, 6(2), 719-23, 1992

Barnes, H J, Arlotto, M P, Waterman, M R. Expression and enzymatic activity of recombinant cytochrome P450 17 alpha-hydroxylase in Escherichia coli. Proc Natl Acad Sci U S A, 88(13), 5597-601, 1991 PMCID:51924

Biason, A, Mantero, F, Scaroni, C, Simpson, E R, Waterman, M R. Deletion within the CYP17 gene together with insertion of foreign DNA is the cause of combined complete 17 alpha-hydroxylase/17,20-lyase deficiency in an Italian patient. Mol Endocrinol, 5(12), 2037-45, 1991

Clark, B J, Waterman, M R. The hydrophobic amino-terminal sequence of bovine 17 alpha-hydroxylase is required for the expression of a functional hemoprotein in COS 1 cells. J Biol Chem, 266(9), 5898-904, 1991

Clark, B J, Waterman, M R. Heterologous expression of mammalian P450 in COS cells. Methods Enzymol, 206, 100-8, 1991

Ehrhart-Bornstein, M, Bornstein, S R, Trzeclak, W H, Usadel, H, G??se-Behling, H, Waterman, M R, Scherbaum, W A. Adrenaline stimulates cholesterol side-chain cleavage cytochrome P450 mRNA accumulation in bovine adrenocortical cells. J Endocrinol, 131(2), R5-8, 1991

Estabrook, R W, Mason, J I, Simpson, E R, Peterson, J A, Waterman, M R. The heterologous expression of the cytochromes P450: a new approach for the study of enzyme activities and regulation. Adv Enzyme Regul, 31, 365-83, 1991

Kagawa, N, Waterman, M R. Evidence that an adrenal-specific nuclear protein regulates the cAMP responsiveness of the human CYP21B (P450C21) gene. J Biol Chem, 266(17), 11199-204, 1991

Lauber, M E, Bengtson, T, Waterman, M R, Simpson, E R. Regulation of CYP11A (P450SCC) and CYP17 (P450(17) alpha) gene expression in bovine luteal cells in primary culture. J Biol Chem, 266(17), 11170-5, 1991

Lauber, M E, Waterman, M R, Simpson, E R. Expression of genes encoding steroidogenic enzymes in the bovine corpus luteum. J Reprod Fertil Suppl, 43, 57-64, 1991

Stirling, D, Waterman, M R, Simpson, E R. Expression of mRNA encoding basic fibroblast growth factor (bFGF) in bovine corpora lutea and cultured luteal cells. J Reprod Fertil, 91(1), 1-8, 1991

Tremblay, A, Waterman, M R, Parker, K L, Lehoux, J G. Regulation of rat adrenal messenger RNA and protein levels for cytochrome P-450s and adrenodoxin by dietary sodium depletion or potassium intake. J Biol Chem, 266(4), 2245-51, 1991

Wada, A, Mathew, P A, Barnes, H J, Sanders, D, Estabrook, R W, Waterman, M R. Expression of functional bovine cholesterol side chain cleavage cytochrome P450 (P450scc) in Escherichia coli. Arch Biochem Biophys, 290(2), 376-80, 1991

Yanase, T, Simpson, E R, Waterman, M R. 17 alpha-hydroxylase/17,20-lyase deficiency: from clinical investigation to molecular definition. Endocr Rev, 12(1), 91-108, 1991

Zanger, U M, Lund, J, Simpson, E R, Waterman, M R. Activation of transcription in cell-free extracts by a novel cAMP-responsive sequence from the bovine CYP17 gene. J Biol Chem, 266(18), 11417-20, 1991

Ahlgren, R, Simpson, E R, Waterman, M R, Lund, J. Characterization of the promoter/regulatory region of the bovine CYP11A (P-450scc) gene. Basal and cAMP-dependent expression. J Biol Chem, 265(6), 3313-9, 1990

Kagawa, N, Waterman, M R. cAMP-dependent transcription of the human CYP21B (P-450C21) gene requires a cis-regulatory element distinct from the consensus cAMP-regulatory element. J Biol Chem, 265(19), 11299-305, 1990

Lorence, M C, Trant, J M, Clark, B J, Khyatt, B, Mason, J I, Estabrook, R W, Waterman, M R. Construction and expression of human/bovine P45017 alpha chimeric proteins: evidence for distinct tertiary structures in the same P450 from two different species. Biochemistry, 29(42), 9819-24, 1990

Lund, J, Ahlgren, R, Wu, D H, Kagimoto, M, Simpson, E R, Waterman, M R. Transcriptional regulation of the bovine CYP17 (P-450(17)alpha) gene. Identification of two cAMP regulatory regions lacking the consensus cAMP-responsive element (CRE). J Biol Chem, 265(6), 3304-12, 1990

Mathew, P A, Kagawa, N, Bhasker, C R, Waterman, M R. Deduced amino acid sequence of heme binding region of chicken cholesterol side chain cleavage cytochrome P450. Protein Seq Data Anal, 3(4), 323-5, 1990

Mathew, P A, Mason, J I, Trant, J M, Sanders, D, Waterman, M R. Amino acid substitutions Phe66----Leu and Ser126----Pro abolish cortisol and aldosterone synthesis by bovine cytochrome P450(11)beta. J Biol Chem, 265(33), 20228-33, 1990

Mathew, P A, Mason, J I, Trant, J M, Waterman, M R. Incorporation of steroidogenic pathways which produce cortisol and aldosterone from cholesterol into nonsteroidogenic cells. Mol Cell Endocrinol, 73(1), 73-80, 1990

McAllister, J M, Mason, J I, Byrd, W, Trant, J M, Waterman, M R, Simpson, E R. Proliferating human granulosa-lutein cells in long term monolayer culture: expression of aromatase, cholesterol side-chain cleavage, and 3 beta-hydroxysteroid dehydrogenase. J Clin Endocrinol Metab, 71(1), 26-33, 1990

Simpson, E R, Lund, J, Ahlgren, R, Waterman, M R. Regulation by cyclic AMP of the genes encoding steroidogenic enzymes: when the light finally shines. Mol Cell Endocrinol, 70(2), C25-8, 1990

Stirling, D, Magness, R R, Stone, R, Waterman, M R, Simpson, E R. Angiotensin II inhibits luteinizing hormone-stimulated cholesterol side chain cleavage expression and stimulates basic fibroblast growth factor expression in bovine luteal cells in primary culture. J Biol Chem, 265(1), 5-8, 1990

Waterman, M R, Lund, J, Ahlgren, R, Wu, D, Simpson, E R. Regulation of steroid hydroxylase gene expression and steroid hormone biosynthesis. Biochem Soc Trans, 18(1), 26-8, 1990

Waterman, M R, Simpson, E R. Steroidogenic capacity in the adrenal cortex and its regulation. Prog Drug Res, 34, 359-81, 1990

Yanase, T, Sanders, D, Shibata, A, Matsui, N, Simpson, E R, Waterman, M R. Combined 17 alpha-hydroxylase/17,20-lyase deficiency due to a 7-basepair duplication in the N-terminal region of the cytochrome P45017 alpha (CYP17) gene. J Clin Endocrinol Metab, 70(5), 1325-9, 1990

Bhasker, C R, Adler, B S, Dee, A, John, M E, Kagimoto, M, Zuber, M X, Ahlgren, R, Wang, X D, Simpson, E R, Waterman, M R. Structural characterization of the bovine CYP17 (17 alpha-hydroxylase) gene. Arch Biochem Biophys, 271(2), 479-87, 1989

Boggaram, V, John, M E, Simpson, E R, Waterman, M R. Effect of ACTH on the stability of mRNAs encoding bovine adrenocortical P-450scc, P-45011 beta, P-45017 alpha, P-450C21 and adrenodoxin. Biochem Biophys Res Commun, 160(3), 1227-32, 1989

Fevold, H R, Lorence, M C, McCarthy, J L, Trant, J M, Kagimoto, M, Waterman, M R, Mason, J I. Rat P450(17 alpha) from testis: characterization of a full-length cDNA encoding a unique steroid hydroxylase capable of catalyzing both delta 4- and delta 5-steroid-17,20-lyase reactions. Mol Endocrinol, 3(6), 968-75, 1989

Kagimoto, K, Waterman, M R, Kagimoto, M, Ferreira, P, Simpson, E R, Winter, J S. Identification of a common molecular basis for combined 17 alpha-hydroxylase/17,20-lyase deficiency in two Mennonite families. Hum Genet, 82(3), 285-6, 1989

Lorence, M C, Trant, J M, Mason, J I, Bhasker, C R, Fujii-Kuriyama, Y, Estabrook, R W, Waterman, M R. Expression of a full-length cDNA encoding bovine adrenal cytochrome P450C21. Arch Biochem Biophys, 273(1), 79-88, 1989

Lund, J, Mason, J I, Simpson, E R, Waterman, M R. Developmental regulation of P-45017 alpha gene expression in fetal bovine adrenal. Endocr Res, 15(1-2), 17-29, 1989

McAllister, J M, Kerin, J F, Trant, J M, Estabrook, R W, Mason, J I, Waterman, M R, Simpson, E R. Regulation of cholesterol side-chain cleavage and 17 alpha-hydroxylase/lyase activities in proliferating human theca interna cells in long term monolayer culture. Endocrinology, 125(4), 1959-66, 1989

Swart, P, Estabrook, R W, Mason, J I, Waterman, M R. Catalytic activity of human and bovine adrenal cytochromes P-450 17 alpha, lyase expressed in Cos 1 cells. Biochem Soc Trans, 17(6), 1025-6, 1989

Waterman, M R, Mason, J I, Zuber, M X, Lorence, M C, Clark, B J, Trant, J M, Barnes, H J, Simpson, E R, Estabrook, R W. Expression of P-450 enzyme activities in heterologous cells by transfection. Arch Toxicol Suppl, 13, 155-63, 1989

Waterman, M R, Simpson, E R. Regulation of steroid hydroxylase gene expression is multifactorial in nature. Recent Prog Horm Res, 45, 533-63; discussion 563-6, 1989

Yanase, T, Kagimoto, M, Suzuki, S, Hashiba, K, Simpson, E R, Waterman, M R. Deletion of a phenylalanine in the N-terminal region of human cytochrome P-450(17 alpha) results in partial combined 17 alpha-hydroxylase/17,20-lyase deficiency. J Biol Chem, 264(30), 18076-82, 1989

Brownie, A C, Bhasker, C R, Waterman, M R. Levels of adrenodoxin, NADPH-cytochrome P-450 reductase and cytochromes P-45011 beta, P-45021, P-450scc, in adrenal zona fasciculata-reticularis tissue from androgen-treated rats. Mol Cell Endocrinol, 55(1), 15-20, 1988

Estabrook, R W, Mason, J I, Martin-Wixtrom, C, Zuber, M, Waterman, M R. Some enzymatic vagaries of a bovine adrenal microsomal cytochrome P-450 introduced and expressed in transformed monkey kidney cells. Prog Clin Biol Res, 274, 525-40, 1988

Kagimoto, K, McCarthy, J L, Waterman, M R, Kagimoto, M. Deduced amino acid sequence of mature chicken testis ferredoxin. Biochem Biophys Res Commun, 155(1), 379-83, 1988

Kagimoto, M, Kagimoto, K, Simpson, E R, Waterman, M R. Transcription of the bovine adrenodoxin gene produces two species of mRNA of which only one is translated into adrenodoxin. J Biol Chem, 263(18), 8925-8, 1988

Kagimoto, M, Winter, J S, Kagimoto, K, Simpson, E R, Waterman, M R. Structural characterization of normal and mutant human steroid 17 alpha-hydroxylase genes: molecular basis of one example of combined 17 alpha-hydroxylase/17,20 lyase deficiency. Mol Endocrinol, 2(6), 564-70, 1988

Lund, J, Faucher, D J, Ford, S P, Porter, J C, Waterman, M R, Mason, J I. Developmental expression of bovine adrenocortical steroid hydroxylases. Regulation of P-450(17 alpha) expression leads to episodic fetal cortisol production. J Biol Chem, 263(31), 16195-201, 1988

McCarthy, J L, Waterman, M R. Co-induction of 17 alpha-hydroxylase and C-17,20-lyase activities in primary cultures of bovine adrenocortical cells in response to ACTH treatment. J Steroid Biochem, 29(3), 307-12, 1988

Nishihara, M, Winters, C A, Buzko, E, Waterman, M R, Dufau, M L. Hormonal regulation of rat Leydig cell cytochrome P-45017 alpha mRNA levels and characterization of a partial length rat P-45017 alpha cDNA. Biochem Biophys Res Commun, 154(1), 151-8, 1988

Rodgers, R J, Waterman, M R, Simpson, E R, Magness, R R. Immunoblot analysis of cholesterol side-chain cleavage cytochrome P-450 and adrenodoxin in corpora lutea of cyclic and late-pregnant sheep. J Reprod Fertil, 83(2), 843-50, 1988

Simpson, E R, Waterman, M R. Regulation of the synthesis of steroidogenic enzymes in adrenal cortical cells by ACTH. Annu Rev Physiol, 50, 427-40, 1988

Waterman, M R, Mason, J I, Simpson, E R. Regulation of steroid hydroxylase gene expression. Prog Clin Biol Res, 274, 543-55, 1988

Yanase, T, Kagimoto, M, Matsui, N, Simpson, E R, Waterman, M R. Combined 17 alpha-hydroxylase/17,20-lyase deficiency due to a stop codon in the N-terminal region of 17 alpha-hydroxylase cytochrome P-450. Mol Cell Endocrinol, 59(3), 249-53, 1988

Zuber, M X, Mason, J I, Simpson, E R, Waterman, M R. Simultaneous transfection of COS-1 cells with mitochondrial and microsomal steroid hydroxylases: incorporation of a steroidogenic pathway into nonsteroidogenic cells. Proc Natl Acad Sci U S A, 85(3), 699-703, 1988 PMCID:279622

Bhasker, C R, Okamura, T, Simpson, E R, Waterman, M R. Mature bovine adrenodoxin contains a 14-amino-acid COOH-terminal extension originally detected by cDNA sequencing. Eur J Biochem, 164(1), 21-5, 1987

Bradshaw, K D, Waterman, M R, Couch, R T, Simpson, E R, Zuber, M X. Characterization of complementary deoxyribonucleic acid for human adrenocortical 17 alpha-hydroxylase: a probe for analysis of 17 alpha-hydroxylase deficiency. Mol Endocrinol, 1(5), 348-54, 1987

John, M E, Simpson, E R, Carr, B R, Magness, R R, Rosenfeld, C R, Waterman, M R, Mason, J I. Ontogeny of adrenal steroid hydroxylases: evidence for cAMP-independent gene expression. Mol Cell Endocrinol, 50(3), 263-8, 1987

Muerhoff, A S, Williams, D E, Leithauser, M T, Jackson, V E, Waterman, M R, Masters, B S. Regulation of the induction of a cytochrome P-450 prostaglandin omega-hydroxylase by pregnancy in rabbit lung. Proc Natl Acad Sci U S A, 84(22), 7911-4, 1987 PMCID:299445

Okamura, T, Kagimoto, M, Simpson, E R, Waterman, M R. Multiple species of bovine adrenodoxin mRNA. Occurrence of two different mitochondrial precursor sequences associated with the same mature sequence. J Biol Chem, 262(21), 10335-8, 1987

Rodgers, R J, Mason, J I, Waterman, M R, Simpson, E R. Regulation of the synthesis of 3-hydroxy-3-methylglutaryl coenzyme A reductase in the bovine ovary in vivo and in vitro. Mol Endocrinol, 1(2), 172-80, 1987

Rodgers, R J, Waterman, M R, Simpson, E R. Levels of messenger ribonucleic acid encoding cholesterol side-chain cleavage cytochrome P-450, 17 alpha-hydroxylase cytochrome P-450, adrenodoxin, and low density lipoprotein receptor in bovine follicles and corpora lutea throughout the ovarian cycle. Mol Endocrinol, 1(3), 274-9, 1987

Simpson, E R, Mason, J I, John, M E, Zuber, M X, Rodgers, R J, Waterman, M R. Regulation of the biosynthesis of steroidogenic enzymes. J Steroid Biochem, 27(4-6), 801-5, 1987

Trzeciak, W H, Duda, T, Waterman, M R, Simpson, E R. Effects of epidermal growth factor on the synthesis of the cholesterol side-chain cleavage enzyme complex in rat ovarian granulosa cells in primary culture. Mol Cell Endocrinol, 52(1-2), 43-50, 1987

Trzeciak, W H, Duda, T, Waterman, M R, Simpson, E R. Tetradecanoyl phorbol acetate suppresses follicle-stimulating hormone-induced synthesis of the cholesterol side-chain cleavage enzyme complex in rat ovarian granulosa cells. J Biol Chem, 262(31), 15246-50, 1987

Trzeciak, W H, Simpson, E R, Scallen, T J, Vahouny, G V, Waterman, M R. Studies on the synthesis of sterol carrier protein-2 in rat adrenocortical cells in monolayer culture. Regulation by ACTH and dibutyryl cyclic 3',5'-AMP. J Biol Chem, 262(8), 3713-7, 1987

Trzeciak, W H, Waterman, M R, Simpson, E R, Ojeda, S R. Vasoactive intestinal peptide regulates cholesterol side-chain cleavage cytochrome P-450 (P-450scc) gene expression in granulosa cells from immature rat ovaries. Mol Endocrinol, 1(7), 500-4, 1987

John, M E, John, M C, Boggaram, V, Simpson, E R, Waterman, M R. Transcriptional regulation of steroid hydroxylase genes by corticotropin. Proc Natl Acad Sci U S A, 83(13), 4715-9, 1986 PMCID:323812

John, M E, Okamura, T, Dee, A, Adler, B, John, M C, White, P C, Simpson, E R, Waterman, M R. Bovine steroid 21-hydroxylase: regulation of biosynthesis. Biochemistry, 25(10), 2846-53, 1986

John, M E, Simpson, E R, Waterman, M R, Mason, J I. Regulation of cholesterol side-chain cleavage cytochrome P-450 gene expression in adrenal cells in monolayer culture. Mol Cell Endocrinol, 45(2-3), 197-204, 1986

Matocha, M F, Waterman, M R. Import and processing of P-450SCC and P-450(11) beta precursors by corpus luteal mitochondria: a processing pathway recognizing homologous and heterologous precursors. Arch Biochem Biophys, 250(2), 456-60, 1986

Rodgers, R J, Rodgers, H F, Hall, P F, Waterman, M R, Simpson, E R. Immunolocalization of cholesterol side-chain-cleavage cytochrome P-450 and 17 alpha-hydroxylase cytochrome P-450 in bovine ovarian follicles. J Reprod Fertil, 78(2), 627-38, 1986

Rodgers, R J, Rodgers, H F, Waterman, M R, Simpson, E R. Immunolocalization of cholesterol side-chain-cleavage cytochrome P-450 and ultrastructural studies of bovine corpora lutea. J Reprod Fertil, 78(2), 639-52, 1986

Rodgers, R J, Waterman, M R, Simpson, E R. Cytochromes P-450scc, P-450(17)alpha, adrenodoxin, and reduced nicotinamide adenine dinucleotide phosphate-cytochrome P-450 reductase in bovine follicles and corpora lutea. Changes in specific contents during the ovarian cycle. Endocrinology, 118(4), 1366-74, 1986

Sasaki, J, Waterman, M R, Cottam, G L. Decreased apolipoprotein A-I and B content in plasma of individuals with sickle cell anemia. Clin Chem, 32(1 Pt 1), 226-7, 1986

Trzeciak, W H, Waterman, M R, Simpson, E R. Synthesis of the cholesterol side-chain cleavage enzymes in cultured rat ovarian granulosa cells: induction by follicle-stimulating hormone and dibutyryl adenosine 3',5'-monophosphate. Endocrinology, 119(1), 323-30, 1986

Waterman, M R, Mason, J I, Zuber, M X, John, M E, Rodgers, R J, Simpson, E R. Control of gene expression of adrenal steroid hydroxylases and related enzymes. Endocr Res, 12(4), 393-408, 1986

Zuber, M X, John, M E, Okamura, T, Simpson, E R, Waterman, M R. Bovine adrenocortical cytochrome P-450(17 alpha). Regulation of gene expression by ACTH and elucidation of primary sequence. J Biol Chem, 261(5), 2475-82, 1986

Zuber, M X, Simpson, E R, Waterman, M R. Expression of bovine 17 alpha-hydroxylase cytochrome P-450 cDNA in nonsteroidogenic (COS 1) cells. Science, 234(4781), 1258-61, 1986

Boggaram, V, Horowitz, P, Waterman, M R. Studies on rhodanese synthesis in bovine adrenocortical cells. Biochem Biophys Res Commun, 130(1), 407-11, 1985

Dee, A, Carlson, G, Smith, C, Masters, B S, Waterman, M R. Regulation of synthesis and activity of bovine adrenocortical NADPH-cytochrome P-450 reductase by ACTH. Biochem Biophys Res Commun, 128(2), 650-6, 1985

John, M E, John, M C, Simpson, E R, Waterman, M R. Regulation of cytochrome P-45011 beta gene expression by adrenocorticotropin. J Biol Chem, 260(9), 5760-7, 1985

Marston, S A, Waterman, M R, McCarthy, J L. 3 Beta-hydroxysteroid dehydrogenase-isomerase activity in bovine adrenocortical cells in culture: lack of response to ACTH treatment. J Steroid Biochem, 22(6), 789-94, 1985

Matocha, M F, Waterman, M R. Synthesis and processing of mitochondrial steroid hydroxylases. In vivo maturation of the precursor forms of cytochrome P-450scc, cytochrome P-450(11)beta, and adrenodoxin. J Biol Chem, 260(22), 12259-65, 1985

Okamura, T, John, M E, Zuber, M X, Simpson, E R, Waterman, M R. Molecular cloning and amino acid sequence of the precursor form of bovine adrenodoxin: evidence for a previously unidentified COOH-terminal peptide. Proc Natl Acad Sci U S A, 82(17), 5705-9, 1985 PMCID:390620

Sheets, J J, Zuber, M X, McCarthy, J L, Vickery, L E, Waterman, M R. Discriminatory inhibition of adrenocortical 17 alpha-hydroxylase activity by inhibitors of cholesterol side chain cleavage cytochrome P-450. Arch Biochem Biophys, 242(1), 297-305, 1985

Simpson, E R, Boggaram, V, Funkenstein, B, Waterman, M R. Lipoproteins and the regulation of steroidogenesis. Biochem Soc Trans, 13(1), 55-8, 1985

Waterman, M R, Simpson, E R. Regulation of the biosynthesis of cytochromes P-450 involved in steroid hormone synthesis. Mol Cell Endocrinol, 39(2), 81-9, 1985

Zuber, M X, Simpson, E R, Hall, P F, Waterman, M R. Effects of adrenocorticotropin on 17 alpha-hydroxylase activity and cytochrome P-450(17 alpha) synthesis in bovine adrenocortical cells. J Biol Chem, 260(3), 1842-8, 1985

Zuber, M X, Simpson, E R, Waterman, M R. Regulation of cytochrome P-450(17)alpha activity and synthesis in bovine adrenocortical cells. Ann N Y Acad Sci, 458, 252-61, 1985

Boggaram, V, Funkenstein, B, Waterman, M R, Simpson, E R. Lipoproteins and the regulation of adrenal steroidogenesis. Endocr Res, 10(3-4), 387-409, 1984

Boggaram, V, Simpson, E R, Waterman, M R. Induction of synthesis of bovine adrenocortical cytochromes P-450scc, P-45011 beta, P-450C21, and adrenodoxin by prostaglandins E2 and F2 alpha and cholera toxin. Arch Biochem Biophys, 231(2), 271-9, 1984

Boggaram, V, Zuber, M X, Waterman, M R. Turnover of newly synthesized cytochromes P-450scc and P-45011 beta and adrenodoxin in bovine adrenocortical cells in monolayer culture: effect of adrenocorticotropin. Arch Biochem Biophys, 231(2), 518-23, 1984

Funkenstein, B, Waterman, M R, Simpson, E R. Induction of synthesis of cholesterol side chain cleavage cytochrome P-450 and adrenodoxin by follicle-stimulating hormone, 8-bromo-cyclic AMP, and low density lipoprotein in cultured bovine granulosa cells. J Biol Chem, 259(13), 8572-7, 1984

John, M E, John, M C, Ashley, P, MacDonald, R J, Simpson, E R, Waterman, M R. Identification and characterization of cDNA clones specific for cholesterol side-chain cleavage cytochrome P-450. Proc Natl Acad Sci U S A, 81(18), 5628-32, 1984 PMCID:391763

John, M E, Simpson, E R, Boggaram, V, Waterman, M R. Molecular cloning of steroid hydroxylases. Endocr Res, 10(3-4), 319-33, 1984

Kramer, R E, Rainey, W E, Funkenstein, B, Dee, A, Simpson, E R, Waterman, M R. Induction of synthesis of mitochondrial steroidogenic enzymes of bovine adrenocortical cells by analogs of cyclic AMP. J Biol Chem, 259(2), 707-13, 1984

Matocha, M F, Waterman, M R. Discriminatory processing of the precursor forms of cytochrome P-450scc and adrenodoxin by adrenocortical and heart mitochondria. J Biol Chem, 259(13), 8672-8, 1984

Bethlenfalvay, N C, Waterman, M R, Lima, J E, Waldrup, T. Comparative aspects of methemoglobin formation and reduction in opossum (Didelphis virginiana) and human erythrocytes. Comp Biochem Physiol A, 75(4), 635-9, 1983

Funkenstein, B, McCarthy, J L, Dus, K M, Simpson, E R, Waterman, M R. Effect of adrenocorticotropin on steroid 21-hydroxylase synthesis and activity in cultured bovine adrenocortical cells. Increased synthesis in the absence of increased activity. J Biol Chem, 258(15), 9398-405, 1983

Funkenstein, B, Waterman, M R, Masters, B S, Simpson, E R. Evidence for the presence of cholesterol side chain cleavage cytochrome P-450 and adrenodoxin in fresh granulosa cells. Effects of follicle-stimulating hormone and cyclic AMP on cholesterol side chain cleavage cytochrome P-450 synthesis and activity. J Biol Chem, 258(16), 10187-91, 1983

Kramer, R E, McCarthy, J L, Simpson, E R, Waterman, M R. Effects of ACTH on steroidogenesis in bovine adrenocortical cells in primary culture--increased secretion of 17 alpha-hydroxylated steroids associated with a refractoriness in total steroid output. J Steroid Biochem, 18(6), 715-23, 1983

Kramer, R E, Simpson, E R, Waterman, M R. Induction of 11 beta-hydroxylase by corticotropin in primary cultures of bovine adrenocortical cells. J Biol Chem, 258(5), 3000-5, 1983

Kramer, R E, Simpson, E R, Waterman, M R. Steroidogenic refractoriness of bovine adrenocortical cells to dibutyryl cyclic AMP. Steroids, 41(2), 207-23, 1983

McCarthy, J L, Kramer, R E, Funkenstein, B, Simpson, E R, Waterman, M R. Induction of 17 alpha-hydroxylase (cytochrome P-450(17)alpha) activity by adrenocorticotropin in bovine adrenocortical cells maintained in monolayer culture. Arch Biochem Biophys, 222(2), 590-8, 1983

Ohashi, M, Simpson, E R, Mason, J I, Waterman, M R. Biosynthesis of cholesterol side-chain cleavage cytochrome P-450 in human fetal adrenal cells in culture. Endocrinology, 112(6), 2039-45, 1983

Sasaki, J, Waterman, M R, Buchanan, G R, Cottam, G L. Plasma and erythrocyte lipids in sickle cell anaemia. Clin Lab Haematol, 5(1), 35-44, 1983

Sharma, V S, Isaacson, R A, John, M E, Waterman, M R, Chevion, M. Reaction of nitric oxide with heme proteins: studies on metmyoglobin, opossum methemoglobin, and microperoxidase. Biochemistry, 22(16), 3897-902, 1983

Simpson, E R, Waterman, M R. Regulation by ACTH of steroid hormone biosynthesis in the adrenal cortex. Can J Biochem Cell Biol, 61(7), 692-707, 1983

Simpson, E R, Waterman, M R. Induction of synthesis of steroidogenic enzymes by corticotropin in bovine adrenocortical cells in culture. Biochem Soc Trans, 11(6), 653-5, 1983

Waterman, M R, Estabrook, R W. The induction of microsomal electron transport enzymes. Mol Cell Biochem, 53-54(1-2), 267-78, 1983

Bethlenfalvay, N C, Waterman, M R, Lima, J E, Waldrup, T. Cytosolic and membrane-bound methemoglobin reductases in erythrocytes of the opossum, Didelphis virginiana. Comp Biochem Physiol B, 73(3), 591-4, 1982

John, M E, Bethlenfalvay, N C, Waterman, M R. Oxidation-reduction properties of the hemoglobin of the opossum, Didelphis virginiana. Comp Biochem Physiol B, 73(3), 585-90, 1982

Kramer, R E, Anderson, C M, Peterson, J A, Simpson, E R, Waterman, M R. Adrenodoxin biosynthesis by bovine adrenal cells in monolayer culture. Induction by adrenocorticotropin. J Biol Chem, 257(24), 14921-5, 1982

Kramer, R E, Du Bois, R N, Simpson, E R, Anderson, C M, Kashiwagi, K, Lambeth, J D, Jefcoate, C R, Waterman, M R. Cell-free synthesis of precursor forms of mitochondrial steroid hydroxylase enzymes of the bovine adrenal cortex. Arch Biochem Biophys, 215(2), 478-85, 1982

Sharma, V S, John, M E, Waterman, M R. Functional studies on hemoglobin opossum. Conclusions drawn regarding the role of the distal histidine. J Biol Chem, 257(20), 11887-92, 1982

Stone, M J, Willerson, J T, Waterman, M R. Radioimmunoassay of myoglobin. Methods Enzymol, 84, 172-7, 1982

Waterman, M R. ACTH-mediated induction of synthesis and activity of cytochrome P-450s and related enzymes in cultured bovine adrenocortical cells. Xenobiotica, 12(11), 773-86, 1982

DuBois, R N, Simpson, E R, Kramer, R E, Waterman, M R. Induction of synthesis of cholesterol side chain cleavage cytochrome P-450 by adrenocorticotropin in cultured bovine adrenocortical cells. J Biol Chem, 256(13), 7000-5, 1981

DuBois, R N, Simpson, E R, Tuckey, J, Lambeth, J D, Waterman, M R. Evidence for a higher molecular weight precursor of cholesterol side-chain-cleavage cytochrome P-450 and induction of mitochondrial and cytosolic proteins by corticotropin in adult bovine adrenal cells. Proc Natl Acad Sci U S A, 78(2), 1028-32, 1981 PMCID:319939

John, M E, DuBois, R N, Waterman, M R. Altered heme environments in opossum and rabbit methemoglobins. Z Naturforsch [C], 36(11-12), 964-7, 1981

John, M E, Waterman, M R. Spectral, conformational and chemical properties of opossum methemoglobin. Eur J Biochem, 115(1), 1-6, 1981

John, M E, Waterman, M R. Structural basis for the conformational states of nitrosyl hemoglobins M Saskatoon and M Milwaukee. Influence of distal histidine residues on proximal histidine-iron bonds. J Biol Chem, 255(10), 4501-6, 1980

Shibata, K, Cottam, G L, Waterman, M R. Acceleration of the rate of deoxyhemoglobin S polymerization by the erythrocyte membrane. FEBS Lett, 110(1), 107-10, 1980

Dubois, R N, Waterman, M R. Effect of phenobarbital administration to rats on the level of the in vitro synthesis of cytochrome P-450 directed by total rat liver RNA. Biochem Biophys Res Commun, 90(1), 150-7, 1979

Horne, M K, Waterman, M R, Simon, L M, Garriott, J C, Foerster, E H. Methemoglobinemia from sniffing butyl nitrite. Ann Intern Med, 91(3), 417-8, 1979

John, M E, Waterman, M R. Differential effects of pH and inositol hexaphosphate on the spectroscopic properties of the alpha and beta subunits in methemoglobins M Milwaukee and A. Biochim Biophys Acta, 578(2), 269-80, 1979

John, M E, Waterman, M R. Structural characteristics of nitrosyl hemoglobins and their relation to ESR spectra. FEBS Lett, 106(1), 219-22, 1979

John, M E, Waterman, M R. Nitric oxide induced conformational changes in opossum hemoglobin. J Biol Chem, 254(23), 11953-7, 1979

Russo, F M, John, M E, Waterman, M R, Simon, L M, Sheehan, R G. Hemoglobin Hofu (beta 126 Val is replaced by Glu) in a black American. Hemoglobin, 3(1), 83-6, 1979

Waterman, M R, Cottam, G L, Shibata, K. Inhibitory effect of deoxyhemoglobin A2 on the rate of deoxyhemoglobin S polymerization. J Mol Biol, 129(2), 337-41, 1979

Stone, M J, Waterman, M R, Poliner, L R, Templeton, G H, Buja, L M, Willerson, J T. Myoglobinemia is an early and quantitative index of acute myocardial infarction. Angiology, 29(5), 386-92, 1978

Waterman, M R. Spectral characterization of human hemoglobin and its derivatives. Methods Enzymol, 52, 456-63, 1978

Cottam, G L, Waterman, M R, Thompson, B C. Kinetics of polymerization of deoxyhemoglobin S and mixtures of hemoglobin A and hemoglobin S at high hemoglobin concentrations. Arch Biochem Biophys, 181(1), 61-5, 1977

Shibata, K, Waterman, M R, Cottam, G L. Alteration of the trate of deoxyhemoglobin S polymerization. Effect of pH and percentage of oxygenation. J Biol Chem, 252(21), 7468-74, 1977

Stone, M J, Waterman, M R, Harimoto, D, Murray, G, Willson, N, Platt, M R, Blomqvist, G, Willerson, J T. Serum myoglobin level as diagnostic test in patients with acute myocardial infarction. Br Heart J, 39(4), 375-80, 1977 PMCID:483248

Bethlenfalvay, N C, Brown, G L, Waterman, M R. Hemoglobins of the opossum (Didelphis marsupialis). II. Polymorphism; electrophoretic and chromatographic observations. Lab Anim Sci, 26(6 Pt 1), 908-12, 1976

Cottam, G L, Waterman, M R. Effect of oxygen concentration on trasverse water proton relaxation times in erythrocytes homozygous and heterozygous for hemoglonin S. Arch Biochem Biophys, 177(1), 293-8, 1976

Waterman, M R. Effect of carbamidomethylation of cysteine residues G11(104)alpha on the properties of hemoglobin A. Biochim Biophys Acta, 439(1), 167-74, 1976

Waterman, M R, Cottam, G L. Kinetics of the polymerization of hemoglobin S: studies below normal erythrocyte hemoglobin concentration. Biochem Biophys Res Commun, 73(3), 639-45, 1976

Waterman, M R, Cottam, G L. Molecular aspects of sickle cell disease. Angew Chem Int Ed Engl, 15(12), 749-57, 1976

Chuang, A H, Waterman, M R, Yamaoka, K, Cottam, L. Effect of pH, carbamylation and other hemoglobins on deoxyhemoglobin S aggregation inside intact erythrocytes as detected by proton relaxation rate measurements. Arch Biochem Biophys, 167(1), 145-50, 1975

Stone, M J, Willerson, J T, Gomez-Sanchez, C E, Waterman, M R. Radioimmunoassay of myoglobin in human serum. Results in patients with acute myocardial infarction. J Clin Invest, 56(5), 1334-9, 1975 PMCID:301998

Thompson, B C, Waterman, M R, Cottam, G L. Evaluation of the water environments in deoxygenated sickle cells by longitudinal and transverse water proton relaxation rates. Arch Biochem Biophys, 166(1), 193-200, 1975

Waterman, M R, Yanaoka, K, Chuang, A H, Cottam, G L. Anti-sickling nature of dimethyl adipimidate. Biochem Biophys Res Commun, 63(3), 580-7, 1975

Cottam, G L, Valentine, K M, Yamaoka, K, Waterman, M R. The gelation of deoxyhemoglobin S in erythrocytes as detected by transverse water proton relazation measurements. Arch Biochem Biophys, 162(2), 487-92, 1974

Gondko, R, Obrebska, M J, Waterman, M R. Preparation and properties of a hemoglobin containing heme only in gamma subunits. Biochem Biophys Res Commun, 56(2), 444-50, 1974

Schacter, B A, Waterman, M R. Activity of various metalloporphyrin protein complexes with microsomal heme oxygenase. Life Sci, 14(1), 47-53, 1974

Waterman, M R. Role of cysteine residues in hemoglobin structure and function: transfer of p-mercuribenzoate from alpha subunits to beta subunits during tetramer formation. Biochim Biophys Acta, 371(1), 159-67, 1974

Waterman, M R, Stenzel, P. Physical and chemical properties of opossum hemoglobin. A hemoglobin containing glutamine at position 58 (E7) in the alpha subunit. Biochim Biophys Acta, 359(2), 401-10, 1974

Waterman, M R, Yamaoka, K, Dahm, L, Taylor, J, Cottam, G L. Noncovalent modification of deoxyhemoglobin S solubility and erythrocyte sickling. Proc Natl Acad Sci U S A, 71(6), 2222-5, 1974 PMCID:388423

Yamaoka, K, Cottam, G L, Waterman, M R. Spin-label studies at F9(93)beta of deoxyhemoglobin S aggregation. Biochem Biophys Res Commun, 58(4), 1058-65, 1974

Yamaoka, K, Waterman, M R. [Letter: Are HbM Sakai and HbM Hyde Park an identical mutation form?]. Rinsho Ketsueki, 15(7), 819, 1974

Cottam, G L, Waterman, M R. Reversible solubility of deoxyhemoglobin S. Biochem Biophys Res Commun, 54(3), 1157-63, 1973

Painter, T D, Waterman, M R. Effect of ligands on alkaline denaturation of fetal hemoglobin and isolated gamma subunits. Biochem Biophys Res Commun, 55(3), 812-7, 1973

Waterman, M R, Ullrich, V, Estabrook, R W. Effect of substrate on the spin state of cytochrome P-450 in hepatic microsomes. Arch Biochem Biophys, 155(2), 355-60, 1973

Waterman, M R, Mason, H S. Redox properties of liver cytochrome P-450. Arch Biochem Biophys, 150(1), 57-63, 1972

Waterman, M R, Gondko, R, Yonetani, T. Studies on modified hemoglobins. 1. Preparation and properties of a hemoglobin containing heme only in -chains. Arch Biochem Biophys, 145(2), 448-55, 1971

Yonetani, T, Iizuka, T, Waterman, M R. Studies on modified hemoglobins. 3. Spin states of ferric hemoglobin, semi-hemoglobin, and isolated subunit chains. J Biol Chem, 246(24), 7683-9, 1971

Waterman, M R, Mason, H S. The redox potential of liver cytochrome P-450. Biochem Biophys Res Commun, 39(3), 450-4, 1970

Waterman, M R, Yonetani, T. Studies on modified hemoglobins. I. Properties of hybrid hemoglobins containing manganese protoporphyrin IX. J Biol Chem, 245(21), 5847-52, 1970

Yonetani, T, Drott, H R, Leigh, J S, Reed, G H, Waterman, M R, Asakura, T. Electromagnetic properties of hemoproteins. 3. Electron paramagnetic resonance characteristics of iron (III) and manganese (II) protoporphyrins IX and their apohemoprotein complexes in high spin states. J Biol Chem, 245(11), 2998-3003, 1970


Postdoctoral Position Available
No

Postdoctoral Position Details
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Updated Date
10/21/2008