Biomedical Research Education & Training
Faculty Member

Caprioli, Richard M., Ph.D.
Director Mass Spectrometry Center
Professor of Biochemistry
Stanford Moore Chair in Biochemistry
Professor of Pharmacology
Professor of Medicine

Lab Url: http://www.mc.vanderbilt.edu/msrc/

Phone Number: (615) 322-4336

Email Address: richard.m.caprioli@vanderbilt.edu

Caprioli, Richard's picture
Academic history
B.Sc, Columbia University, NY,NY
Ph.D., Columbia University, NY,NY
Postdoc, Purdue University, W. Lafayette, IN

Office Address   Mailing Address

9160 MRB III

9160 MRB III 465 21st Avenue S. 37232-8575


Research Keywords
Peptides | Proteins | Mass Spectrometry,Mass spectroscopy,Proteomics

Research Specialty
Metabolism of cellular peptides

Research Description
The research interests of this laboratory are aimed at the investigation of biological processes involving the synthesis, modification, storage and degradation of certain peptides and proteins using modern mass spectrometric methods of analysis to follow molecular events. In recent years there has been a great amount of interest in investigating the biochemical events involved in the metabolism of peptides, primarily in the brain and gut of mammals, encompassing the enzymatic breakdown of these peptides, their production from peptide and protein precursors, and the disruption of these processes by certain xenobiotics. Modern mass spectrometric techniques are used in these studies, including electrospray and matrix-assisted laser desorption ionization mass spectrometry. This research is generally carried out with two experimental approaches.

The first involves in vivo metabolic studies in live animals (rats) using microdialysis sampling procedures together with molecular identification and quantitation by electrospray tandem mass spectrometry. This experimental approach allows us to measure metabolic events that occur in freely moving animals at a level of 50-100 attomoles in the tissue dialysate. Integrated technology for microdialysis/mass spectrometry utilizes quadrupole mass spectrometry (MS/MS) and micro-electrospray ionization (micro-ESI) for very high sensitivity measurements. The micro-ESI source has been optimized to produce ions from the electrospray process at sub-microliter per min flow rates. The application of combined microdialysis/ESI mass spectrometry is ongoing in four areas, i) Investigation of the kinetics and quantitative aspects of the in vivo metabolism of the neuropeptides and enkephalin neuropeptides, ii) Studies of the kinetics and differential processing of opioid peptide fragment precursors in vivo, iii) Investigation of the in vivo quantitative production and enzymatic degradation of N-acetylaspartyl-glutamate in seizure disorders using a rat model, and iv) Development and measurements of in vivo drug pharmacokinetics, using the drugs haloperidol, valproic acid, cocaine, amphetamine, and the new GABA antagonist n-butyl-3 aminopropylphosphinic acid.

The second experimental approach used in the study of cellular peptide metabolism utilizes new molecular imaging technology that is being developed in our lab. This method involves molecular mapping of animal tissue through the production of ion images obtained from the analysis of mammalian tissue slices by matrix-assisted laser desorption mass spectrometry (MALDI MS)12. This technique permits a tissue section to be mapped in multiple molecular weight values, localizing the molecules in an X, Y coordinate representation of the sample. Ion images are produced by repetitive exposure of the sample to the laser beam, where adjacent spots are irradiated, resulting in an ordered array of mass spectra that are keyed to specific locations in the sample. From one raster of the sample, a specific ion image, at any chosen m/z value, could be produced to give the spatial arrangement of molecules of interest. Current work involves; i) Instrument development involving modifications to an existing commercial MALDI (TOF) mass spectrometer, including changes to the laser optics, target movement system, and instrument control hardware and software, ii) Development of methods for sample preparation and target surface modifications to achieve high sensitivity and high image resolution, with molecular specificity, and iii) Applications to specific research areas involving questions about certain spatial distributions of molecules within specific tissues.

Publications
Aerni, HR, Cornett, DS, Caprioli, RM. High-throughput profiling of formalin-fixed paraffin-embedded tissue using parallel electrophoresis and matrix-assisted laser desorption ionization mass spectrometry. Anal Chem, 81(17), 7490-5, 2009 PMCID:2747050

Grey, AC, Chaurand, P, Caprioli, RM, Schey, KL. MALDI imaging mass spectrometry of integral membrane proteins from ocular lens and retinal tissue. J Proteome Res, 8(7), 3278-83, 2009 PMCID:2715141

Konkle, ME, Hargrove, TY, Kleshchenko, YY, von Kries, JP, Ridenour, W, Uddin, MJ, Caprioli, RM, Marnett, LJ, Nes, WD, Villalta, F, Waterman, MR, Lepesheva, GI. Indomethacin amides as a novel molecular scaffold for targeting Trypanosoma cruzi sterol 14alpha-demethylase. J Med Chem, 52(9), 2846-53, 2009 PMCID:2744100

Mathur, BN, Caprioli, RM, Deutch, AY. Proteomic analysis illuminates a novel structural definition of the claustrum and insula. Cereb Cortex, 19(10), 2372-9, 2009 PMCID:2742595

Xu, BJ, Li, J, Beauchamp, RD, Shyr, Y, Li, M, Washington, MK, Yeatman, TJ, Whitehead, RH, Coffey, RJ, Caprioli, RM. Identification of early intestinal neoplasia protein biomarkers using laser capture microdissection and MALDI MS. Mol Cell Proteomics, 8(5), 936-45, 2009 PMCID:2689774

Andersson, M, Groseclose, MR, Deutch, AY, Caprioli, RM. Imaging mass spectrometry of proteins and peptides: 3D volume reconstruction. Nat Methods, 5(1), 101-8, 2008

Burnum, KE, Tranguch, S, Mi, D, Daikoku, T, Dey, SK, Caprioli, RM. Imaging mass spectrometry reveals unique protein profiles during embryo implantation. Endocrinology, 149(7), 3274-8, 2008 PMCID:2453082

Caldwell, RL, Opalenik, SR, Davidson, JM, Caprioli, RM, Nanney, LB. Tissue profiling MALDI mass spectrometry reveals prominent calcium-binding proteins in the proteome of regenerative MRL mouse wounds. Wound Repair Regen, 16(3), 442-9, 2008

Chaurand, P, Latham, JC, Lane, KB, Mobley, JA, Polosukhin, VV, Wirth, PS, Nanney, LB, Caprioli, RM. Imaging Mass Spectrometry of Intact Proteins from Alcohol-Preserved Tissue Specimens: Bypassing Formalin Fixation. J Proteome Res, 7(8), 3543-3555, 2008

Chaurand, P, Rahman, MA, Hunt, T, Mobley, JA, Gu, G, Latham, JC, Caprioli, RM, Kasper, S. Monitoring mouse prostate development by profiling and imaging mass spectrometry. Mol Cell Proteomics, 7(2), 411-23, 2008

Corbin, BD, Seeley, EH, Raab, A, Feldmann, J, Miller, MR, Torres, VJ, Anderson, KL, Dattilo, BM, Dunman, PM, Gerads, R, Caprioli, RM, Nacken, W, Chazin, WJ, Skaar, EP. Metal chelation and inhibition of bacterial growth in tissue abscesses. Science, 319(5865), 962-5, 2008

Cornett, DS, Frappier, SL, Caprioli, RM. MALDI-FTICR imaging mass spectrometry of drugs and metabolites in tissue. Anal Chem, 80(14), 5648-53, 2008

Groseclose, MR, Massion, PP, Chaurand, P, Caprioli, RM. High-throughput proteomic analysis of formalin-fixed paraffin-embedded tissue microarrays using MALDI imaging mass spectrometry. Proteomics, 8(18), 3715-24, 2008

Hardesty, WM, Caprioli, RM. In situ molecular imaging of proteins in tissues using mass spectrometry. Anal Bioanal Chem, 391(3), 899-903, 2008

Lee, YT, Dimitrova, YN, Schneider, G, Ridenour, WB, Bhattacharya, S, Soss, SE, Caprioli, RM, Filipek, A, Chazin, WJ. Structure of the S100A6 complex with a fragment from the C-terminal domain of Siah-1 interacting protein: a novel mode for S100 protein target recognition. Biochemistry, 47(41), 10921-32, 2008 PMCID:2666074

Manier, ML, Cornett, DS, Hachey, DL, Caprioli, RM. Identification of dimethyldioctadecylammonium ion (m/z 550.6) and related species (m/z 522.6, 494.6) as a source of contamination in mass spectrometry. J Am Soc Mass Spectrom, 19(5), 666-70, 2008 PMCID:2516304

Puolitaival, SM, Burnum, KE, Cornett, DS, Caprioli, RM. Solvent-free matrix dry-coating for MALDI imaging of phospholipids. J Am Soc Mass Spectrom, 19(6), 882-6, 2008 PMCID:2696184

Sanders, ME, Dias, EC, Xu, BJ, Mobley, JA, Billheimer, D, Roder, H, Grigorieva, J, Dowsett, M, Arteaga, CL, Caprioli, RM. Differentiating proteomic biomarkers in breast cancer by laser capture microdissection and MALDI MS. J Proteome Res, 7(4), 1500-7, 2008 PMCID:2738605

Seeley, EH, Caprioli, RM. Molecular imaging of proteins in tissues by mass spectrometry. Proc Natl Acad Sci U S A, 105(47), 18126-31, 2008 PMCID:2587620

Seeley, EH, Oppenheimer, SR, Mi, D, Chaurand, P, Caprioli, RM. Enhancement of Protein Sensitivity for MALDI Imaging Mass Spectrometry After Chemical Treatment of Tissue Sections. J Am Soc Mass Spectrom, 2008 PMCID:2582528

Sinha, TK, Khatib-Shahidi, S, Yankeelov, TE, Mapara, K, Ehtesham, M, Cornett, DS, Dawant, BM, Caprioli, RM, Gore, JC. Integrating spatially resolved three-dimensional MALDI IMS with in vivo magnetic resonance imaging. Nat Methods, 5(1), 57-9, 2008 PMCID:2649801

Caldwell, RL, Opalenik, SR, Davidson, JM, Caprioli, RM, Nanney, LB. Tissue profiling MALDI mass spectrometry reveals prominent calcium-binding proteins in the proteome of regenerative MRL mouse wounds. Wound Repair Regen, 2007

Chaurand, P, Schriver, KE, Caprioli, RM. Instrument design and characterization for high resolution MALDI-MS imaging of tissue sections. J Mass Spectrom, 42(4), 476-89, 2007

Cornett, DS, Reyzer, ML, Chaurand, P, Caprioli, RM. MALDI imaging mass spectrometry: molecular snapshots of biochemical systems. Nat Methods, 4(10), 828-33, 2007

Friedman, DB, Wang, SE, Whitwell, CW, Caprioli, RM, Arteaga, CL. Multivariable difference gel electrophoresis and mass spectrometry: a case study on transforming growth factor-beta and ERBB2 signaling. Mol Cell Proteomics, 6(1), 150-69, 2007

Groseclose, MR, Andersson, M, Hardesty, WM, Caprioli, RM. Identification of proteins directly from tissue: in situ tryptic digestions coupled with imaging mass spectrometry. J Mass Spectrom, 42(2), 254-62, 2007

Herring, KD, Oppenheimer, SR, Caprioli, RM. Direct tissue analysis by matrix-assisted laser desorption ionization mass spectrometry: application to kidney biology. Semin Nephrol, 27(6), 597-608, 2007 PMCID:2180835

M''Koma, AE, Blum, DL, Norris, JL, Koyama, T, Billheimer, D, Motley, S, Ghiassi, M, Ferdowsi, N, Bhowmick, I, Chang, SS, Fowke, JH, Caprioli, RM, Bhowmick, NA. Detection of pre-neoplastic and neoplastic prostate disease by MALDI profiling of urine. Biochem Biophys Res Commun, 353(3), 829-34, 2007 PMCID:2562600

McLean, JA, Ridenour, WB, Caprioli, RM. Profiling and imaging of tissues by imaging ion mobility-mass spectrometry. J Mass Spectrom, 42(8), 1099-105, 2007

Reyzer, ML, Caprioli, RM. MALDI-MS-based imaging of small molecules and proteins in tissues. Curr Opin Chem Biol, 11(1), 29-35, 2007

Yildiz, PB, Shyr, Y, Rahman, JS, Wardwell, NR, Zimmerman, LJ, Shakhtour, B, Gray, WH, Chen, S, Li, M, Roder, H, Liebler, DC, Bigbee, WL, Siegfried, JM, Weissfeld, JL, Gonzalez, AL, Ninan, M, Johnson, DH, Carbone, DP, Caprioli, RM, Massion, PP. Diagnostic accuracy of MALDI mass spectrometric analysis of unfractionated serum in lung cancer. J Thorac Oncol, 2(10), 893-901, 2007

Aerni, HR, Cornett, DS, Caprioli, RM. Automated acoustic matrix deposition for MALDI sample preparation. Anal Chem, 78(3), 827-34, 2006

Amann, JM, Chaurand, P, Gonzalez, A, Mobley, JA, Massion, PP, Carbone, DP, Caprioli, RM. Selective profiling of proteins in lung cancer cells from fine-needle aspirates by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Clin Cancer Res, 12(17), 5142-50, 2006

Chaurand, P, Cornett, DS, Caprioli, RM. Molecular imaging of thin mammalian tissue sections by mass spectrometry. Curr Opin Biotechnol, 17(4), 431-6, 2006

Chaurand, P, Norris, JL, Cornett, DS, Mobley, JA, Caprioli, RM. New developments in profiling and imaging of proteins from tissue sections by MALDI mass spectrometry. J Proteome Res, 5(11), 2889-900, 2006

Cornett, DS, Mobley, JA, Dias, EC, Andersson, M, Arteaga, CL, Sanders, ME, Caprioli, RM. A novel histology-directed strategy for MALDI-MS tissue profiling that improves throughput and cellular specificity in human breast cancer. Mol Cell Proteomics, 5(10), 1975-83, 2006

Khatib-Shahidi, S, Andersson, M, Herman, JL, Gillespie, TA, Caprioli, RM. Direct molecular analysis of whole-body animal tissue sections by imaging MALDI mass spectrometry. Anal Chem, 78(18), 6448-56, 2006

Luka, Z, Ham, AJ, Norris, JL, Yeo, EJ, Yermalitsky, V, Glenn, B, Caprioli, RM, Liebler, DC, Wagner, C. Identification of phosphorylation sites in glycine N-methyltransferase from rat liver. Protein Sci, 15(4), 785-94, 2006 PMCID:2242492

Massion, PP, Caprioli, RM. Proteomic strategies for the characterization and the early detection of lung cancer. J Thorac Oncol, 1(9), 1027-39, 2006

Sk??ld, K, Svensson, M, Nilsson, A, Zhang, X, Nydahl, K, Caprioli, RM, Svenningsson, P, Andr??n, PE. Decreased Striatal Levels of PEP-19 Following MPTP Lesion in the Mouse. J Proteome Res, 5(2), 262-269, 2006

Suzuki, K, Yu, X, Chaurand, P, Araki, Y, Lareyre, JJ, Caprioli, RM, Matusik, RJ, Orgebin-Crist, MC. Epididymis-specific promoter-driven gene targeting: A transcription factor which regulates epididymis-specific gene expression. Mol Cell Endocrinol, 2006

Caldwell, RL, Caprioli, RM. Tissue profiling by mass spectrometry: a review of methodology and applications. Mol Cell Proteomics, 4(4), 394-401, 2005

Caprioli, R. Editorial. J Mass Spectrom, 40(4), 429, 2005

Caprioli, RM. Deciphering protein molecular signatures in cancer tissues to aid in diagnosis, prognosis, and therapy. Cancer Res, 65(23), 10642-5, 2005

Chaurand, P, Schwartz, SA, Reyzer, ML, Caprioli, RM. Imaging mass spectrometry: principles and potentials. Toxicol Pathol, 33(1), 92-101, 2005

Crecelius, AC, Cornett, DS, Caprioli, RM, Williams, B, Dawant, BM, Bodenheimer, B. Three-dimensional visualization of protein expression in mouse brain structures using imaging mass spectrometry. J Am Soc Mass Spectrom, 16(7), 1093-9, 2005

Gao, N, Ishii, K, Mirosevich, J, Kuwajima, S, Oppenheimer, SR, Roberts, RL, Jiang, M, Yu, X, Shappell, SB, Caprioli, RM, Stoffel, M, Hayward, SW, Matusik, RJ. Forkhead box A1 regulates prostate ductal morphogenesis and promotes epithelial cell maturation. Development, 132(15), 3431-43, 2005

Hu, X, Friedman, D, Hill, S, Caprioli, R, Nicholson, W, Powers, AC, Hunter, L, Limbird, LE. Proteomic exploration of pancreatic islets in mice null for the alpha2A adrenergic receptor. J Mol Endocrinol, 35(1), 73-88, 2005

Laurent, C, Levinson, DF, Schwartz, SA, Harrington, PB, Markey, SP, Caprioli, RM, Levitt, P. Direct profiling of the cerebellum by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry: A methodological study in postnatal and adult mouse. J Neurosci Res, 81(5), 613-21, 2005

Ma, S, Hill, KE, Burk, RF, Caprioli, RM. Mass spectrometric determination of selenenylsulfide linkages in rat selenoprotein P. J Mass Spectrom, 40(3), 400-4, 2005

Norris, JL, Hangauer, MJ, Porter, NA, Caprioli, RM. Nonacid cleavable detergents applied to MALDI mass spectrometry profiling of whole cells. J Mass Spectrom, 40(10), 1319-26, 2005

Norris, JL, Porter, NA, Caprioli, RM. Combination detergent/MALDI matrix: functional cleavable detergents for mass spectrometry. Anal Chem, 77(15), 5036-40, 2005

Rahman, SM, Shyr, Y, Yildiz, PB, Gonzalez, AL, Li, H, Zhang, X, Chaurand, P, Yanagisawa, K, Slovis, BS, Miller, RF, Ninan, M, Miller, YE, Franklin, WA, Caprioli, RM, Carbone, DP, Massion, PP. Proteomic patterns of preinvasive bronchial lesions. Am J Respir Crit Care Med, 172(12), 1556-62, 2005 PMCID:2718455

Reyzer, ML, Caprioli, RM. MALDI mass spectrometry for direct tissue analysis: a new tool for biomarker discovery. J Proteome Res, 4(4), 1138-42, 2005

Schwartz, SA, Weil, RJ, Thompson, RC, Shyr, Y, Moore, JH, Toms, SA, Johnson, MD, Caprioli, RM. Proteomic-based prognosis of brain tumor patients using direct-tissue matrix-assisted laser desorption ionization mass spectrometry. Cancer Res, 65(17), 7674-81, 2005

Xie, L, Xu, BJ, Gorska, AE, Shyr, Y, Schwartz, SA, Cheng, N, Levy, S, Bierie, B, Caprioli, RM, Moses, HL. Genomic and proteomic analysis of mammary tumors arising in transgenic mice. J Proteome Res, 4(6), 2088-98, 2005

Xu, BJ, Shyr, Y, Liang, X, Ma, LJ, Donnert, EM, Roberts, JD, Zhang, X, Kon, V, Brown, NJ, Caprioli, RM, Fogo, AB. Proteomic patterns and prediction of glomerulosclerosis and its mechanisms. J Am Soc Nephrol, 16(10), 2967-75, 2005

Zimmerman, LJ, Wernke, GR, Caprioli, RM, Liebler, DC. Identification of protein fragments as pattern features in MALDI-MS analyses of serum. J Proteome Res, 4(5), 1672-80, 2005

Chaurand, Pierre, Sanders, Melinda E, Jensen, Roy A, Caprioli, Richard M. Proteomics in diagnostic pathology: profiling and imaging proteins directly in tissue sections. Am J Pathol, 165(4), 1057-68, 2004

Chaurand, Pierre, Schwartz, Sarah A, Billheimer, Dean, Xu, Baogang J, Crecelius, Anna, Caprioli, Richard M. Integrating histology and imaging mass spectrometry. Anal Chem, 76(4), 1145-55, 2004

Chaurand, Pierre, Schwartz, Sarah A, Caprioli, Richard M. Assessing protein patterns in disease using imaging mass spectrometry. J Proteome Res, 3(2), 245-52, 2004

Friedman, David B, Hill, Salisha, Keller, Jeffrey W, Merchant, Nipun B, Levy, Shawn E, Coffey, Robert J, Caprioli, Richard M. Proteome analysis of human colon cancer by two-dimensional difference gel electrophoresis and mass spectrometry. Proteomics, 4(3), 793-811, 2004

Reyzer, Michelle L, Caldwell, Robert L, Dugger, Teresa C, Forbes, James T, Ritter, Christoph A, Guix, Marta, Arteaga, Carlos L, Caprioli, Richard M. Early changes in protein expression detected by mass spectrometry predict tumor response to molecular therapeutics. Cancer Res, 64(24), 9093-100, 2004

Schwartz, Sarah A, Weil, Robert J, Johnson, Mahlon D, Toms, Steven A, Caprioli, Richard M. Protein profiling in brain tumors using mass spectrometry: feasibility of a new technique for the analysis of protein expression. Clin Cancer Res, 10(3), 981-7, 2004

Chaurand, Pierre, Fouch??court, Sophie, DaGue, Beverly B, Xu, Baogang J, Reyzer, Michelle L, Orgebin-Crist, Marie-Claire, Caprioli, Richard M. Profiling and imaging proteins in the mouse epididymis by imaging mass spectrometry. Proteomics, 3(11), 2221-39, 2003

Fouch??court, S, Lareyre, JJ, Chaurand, P, DaGue, BB, Suzuki, K, Ong, DE, Olson, GE, Matusik, RJ, Caprioli, RM, Orgebin-Crist, MC. Identification, immunolocalization, regulation, and postnatal development of the lipocalin EP17 (epididymal protein of 17 kilodaltons) in the mouse and rat epididymis. Endocrinology, 144(3), 887-900, 2003

Ma, S, Caprioli, RM, Hill, KE, Burk, RF. Loss of selenium from selenoproteins: conversion of selenocysteine to dehydroalanine in vitro. J Am Soc Mass Spectrom, 14(6), 593-600, 2003

Ma, S, Hill, KE, Burk, RF, Caprioli, RM. Mass spectrometric identification of N- and O-glycosylation sites of full-length rat selenoprotein P and determination of selenide-sulfide and disulfide linkages in the shortest isoform. Biochemistry, 42(32), 9703-11, 2003

Norris, Jeremy L, Porter, Ned A, Caprioli, Richard M. Mass spectrometry of intracellular and membrane proteins using cleavable detergents. Anal Chem, 75(23), 6642-7, 2003

Phillip Hochgesang, G, Nemeth-Cawley, JF, Rowlinson, SW, Caprioli, RM, Marnett, LJ. Functional analysis of the molecular determinants of cyclooxygenase-2 acetylation by 2-acetoxyphenylhept-2-ynyl sulfide. Arch Biochem Biophys, 409(1), 127-33, 2003

Reyzer, Michelle L, Hsieh, Yunsheng, Ng, Kwokei, Korfmacher, Walter A, Caprioli, Richard M. Direct analysis of drug candidates in tissue by matrix-assisted laser desorption/ionization mass spectrometry. J Mass Spectrom, 38(10), 1081-92, 2003

Schwartz, SA, Reyzer, ML, Caprioli, RM. Direct tissue analysis using matrix-assisted laser desorption/ionization mass spectrometry: practical aspects of sample preparation. J Mass Spectrom, 38(7), 699-708, 2003

Yanagisawa, Kiyoshi, Shyr, Yu, Xu, Baogang J, Massion, Pierre P, Larsen, Paul H, White, Bill C, Roberts, John R, Edgerton, Mary, Gonzalez, Adriana, Nadaf, Sorena, Moore, Jason H, Caprioli, Richard M, Carbone, David P. Proteomic patterns of tumour subsets in non-small-cell lung cancer. Lancet, 362(9382), 433-9, 2003

Yanagisawa, Kiyoshi, Xu, Baogang J, Carbone, David P, Caprioli, Richard M. Molecular fingerprinting in human lung cancer. Clin Lung Cancer, 5(2), 113-8, 2003

Zhen, Y, Caprioli, RM, Staros, JV. Characterization of glycosylation sites of the epidermal growth factor receptor. Biochemistry, 42(18), 5478-92, 2003

Boutaud, O, Ou, JJ, Chaurand, P, Caprioli, RM, Montine, TJ, Oates, JA. Prostaglandin H2 (PGH2) accelerates formation of amyloid beta1-42 oligomers. J Neurochem, 82(4), 1003-6, 2002

Chaurand, P, Caprioli, RM. Direct profiling and imaging of peptides and proteins from mammalian cells and tissue sections by mass spectrometry. Electrophoresis, 23(18), 3125-35, 2002

Chaurand, P, Schwartz, SA, Caprioli, RM. Imaging mass spectrometry: a new tool to investigate the spatial organization of peptides and proteins in mammalian tissue sections. Curr Opin Chem Biol, 6(5), 676-81, 2002

Fouch??court, S, Chaurand, P, DaGue, BB, Lareyre, JJ, Matusik, RJ, Caprioli, RM, Orgebin-Crist, MC. Epididymal lipocalin-type prostaglandin D2 synthase: identification using mass spectrometry, messenger RNA localization, and immunodetection in mouse, rat, hamster, and monkey. Biol Reprod, 66(2), 524-33, 2002

Ma, S, Hill, KE, Caprioli, RM, Burk, RF. Mass spectrometric characterization of full-length rat selenoprotein P and three isoforms shortened at the C terminus. Evidence that three UGA codons in the mRNA open reading frame have alternative functions of specifying selenocysteine insertion or translation termination. J Biol Chem, 277(15), 12749-54, 2002

Xu, BJ, Caprioli, RM, Sanders, ME, Jensen, RA. Direct analysis of laser capture microdissected cells by MALDI mass spectrometry. J Am Soc Mass Spectrom, 13(11), 1292-7, 2002

Boutaud, O, Brame, CJ, Chaurand, P, Li, J, Rowlinson, SW, Crews, BC, Ji, C, Marnett, LJ, Caprioli, RM, Roberts, LJ, Oates, JA. Characterization of the lysyl adducts of prostaglandin H-synthases that are derived from oxygenation of arachidonic acid. Biochemistry, 40(23), 6948-55, 2001

Boutaud, O., Brame, C.J., Chaurand, P., Li, J., Rowlinson, S.W., Crews, B.C., Ji, C., Marnett, L.J., Caprioli, R.M., Roberts, LJ II, Oates, J.A. Characterization of the Lysyl-Adducts of Prostaglandin H-Synthases that are Derived from Oxygenation of Arachidonic Acid. Biochemistry, 40(23), 6948-6955, 2001

Chaurand, P, DaGue, BB, Ma, S, Kasper, S, Caprioli, RM. Strain-based sequence variations and structure analysis of murine prostate specific spermine binding protein using mass spectrometry. Biochemistry, 40(32), 9725-33, 2001

Chaurand, P, DaGue, BB, Pearsall, RS, Threadgill, DW, Caprioli, RM. Profiling proteins from azoxymethane-induced colon tumors at the molecular level by matrix-assisted laser desorption/ionization mass spectrometry. Proteomics, 1(10), 1320-6, 2001

Chaurand, P., DaGue, B.B., Pearsall, R. S., Threadgill, D.W., Caprioli, R.M. Profiling Proteins from Azoxymethane-induced Colon Tumors at the Molecular Level by MALDI Mass Spectrometry. Proteomics, 1, 1320-1326, 2001

Hondal, RJ, Ma, S, Caprioli, RM, Hill, KE, Burk, RF. Heparin-binding histidine and lysine residues of rat selenoprotein P. J Biol Chem, 276(19), 15823-31, 2001

Masumori, N, Thomas, TZ, Chaurand, P, Case, T, Paul, M, Kasper, S, Caprioli, RM, Tsukamoto, T, Shappell, SB, Matusik, RJ. A probasin-large T antigen transgenic mouse line develops prostate adenocarcinoma and neuroendocrine carcinoma with metastatic potential. Cancer Res, 61(5), 2239-49, 2001

Miller, M.A., Nguyen, V.Q., Lee, M-H, Kosinski, M., Schedl, T., Caprioli, R.M., Greenstein, D. A Sperm Cytoskeletal Protein that Signals Oocyte Meiotic Maturation and Ovulation. Science, 291, 2144-2147, 2001

Miller, MA, Nguyen, VQ, Lee, MH, Kosinski, M, Schedl, T, Caprioli, RM, Greenstein, D. A sperm cytoskeletal protein that signals oocyte meiotic maturation and ovulation. Science, 291(5511), 2144-7, 2001

Nemeth, JF, Hochgesang, GP, Marnett, LJ, Caprioli, RM, Hochensang, GP. Characterization of the glycosylation sites in cyclooxygenase-2 using mass spectrometry. Biochemistry, 40(10), 3109-16, 2001

Nemeth, JF, Hochgesang, Jr., GP, Marnett, LJ, Caprioli, RM. Characterization of the Glycosylation Sites in Cyclooxygenase-2 Using Mass Spectrometry. Biochemistry, 10, 3109, 2001

Nguyen, VQ, Caprioli, RM, Cover, TL. Carboxy-terminal proteolytic processing of Helicobacter pylori vacuolating toxin. Infect Immun, 69(1), 543-6, 2001 PMCID:97915

Stoeckli, M, Chaurand, P, Hallahan, DE, Caprioli, RM. Imaging Mass Spectrometry: A New Technique Applied to Mammalian Brain Tumor Protein Expression. Nature Medicine, 7, 493-496, 2001

Stoeckli, M, Chaurand, P, Hallahan, DE, Caprioli, RM. Imaging mass spectrometry: a new technology for the analysis of protein expression in mammalian tissues. Nat Med, 7(4), 493-6, 2001

Todd, P.J., Schaaff, T.G., Chaurand, P., and Caprioli, R.M. Organic Ion Imaging of Biological Tissue with MALDI and SIMS. J. Mass Spectrom., 36(4), 355-369, 2001

Koomen, J.M., Stoeckli, M., and Caprioli, R.M. Mapping of surrogate markers of cellular components and structures using laser desorption mass spectrometry. J. Mass Spectrom, 35, 258-564, 2000

Andren, P.E., and Caprioli, R.M. Determination of extracellular release of neurotensin rat brain regions utilizing in vivo microdialysis/electrospray mass spectrometry. Brian Research , 845, 123-129, 1999

Chaurand, P, Stoeckli, M, Caprioli, RM. Direct profiling of proteins in biological tissue sections by MALDI mass spectrometry. Anal Chem, 71(23), 5263-70, 1999

Chaurand, P., Stoeckli, M. and Caprioli, R.M. Direct Profiling of Proteins in Biological Tissue Sections by MALDI Mass Spectrometry. Anal Chem, 71, 5263-5270, 1999

Schlunegger, UP, Stoeckli, M, Caprioli, RM. Frequency scan for the analysis of high mass ions generated by matrix-assisted laser desorption/ionization in a paul tra. Rapid Commun Mass Spectrom, 13(18), 1792-6, 1999

Stoeckli, M, Farmer, TB, Caprioli, RM. Automated mass spectrometry imaging with a matrix-assisted laser desorption ionization time-of-flight instrument. J Am Soc Mass Spectrom, 10(1), 67-71, 1999

Stoeckli, M., Farmer, T.B., and Caprioli, R.M. Automated MS imaging with a MALDI TOF instrument. J. Am. Soc. Mass Spectrom, 10(1), 67-71, 1999

Zhang, H, Stoeckli, M, Andren, PE, Caprioli, RM. Combining solid-phase preconcentration, capillary electrophoresis and off-line matrix-assisted laser desorption/ionization mass spectrometry: intracerebral metabolic processing of peptide E in vivo. J Mass Spectrom, 34(4), 377-83, 1999

Zhang, H., Stoeckli, M., Andren, P.E., and Caprioli, R.M. Combining solid phase preconcentration, capillary electrophoresis, and imaging matrix-assisted laser desorption ionization mass spectrometry: Intracerebral metabolic processing of peptide E in vivo. J. Mass Spectrom, 342, 377-383, 1999

Andren, P.E., Emmett, M.R., DaGue, B.B., Steulet, A.-F., Waldmeier, P., and Caprioli, R.M. Blood-brain barrier penetration of 3-aminopropyl-n-butylphosphinic acid (CGP 36742) in rat brain by microdialysis/mass spectrometry. J. Mass Spectrom, 33, 281-287, 1998

Farmer, T.B., and Caprioli, R.M. Determination of protein-protein interactions by matrix-assisted laser desorption/ionization mass spectrometry. J. Mass Spectrom, 33(8), 697-704, 1998

Farmer, TB, Caprioli, RM. Determination of protein-protein interactions by matrix-assisted laser desorption/ionization mass spectrometry. J Mass Spectrom, 33(8), 697-704, 1998

Caprioli, R. M., Farmer, T. B., and Gile, J. Molecular imaging of biological samples: localization of peptides and proteins using MALDI-TOF MS. Analytical Chemistry, 69, 4751-4760, 1997

Eriksson, U., Andren, P.E., Caprioli, R.M., and Nyberg, F. Reversed-phase high-performance liquid chromatography combined with tandem mass spectrometry in studies of a substance P-converting enzyme from human cerebrospinal fluid. J. Chromatogr., 743, 213-220, 1996

Sanderson, K., Andren, P.E., Caprioli, R.M., and Nyberg, F. In vitro metabolism of LVV-hemorphin-7 in human plasma studied by reversed-phase high-performance liquid chromatography and micro-electrospray mass spectrometry. J. Chromatogr., 743, 207-212, 1996

Zhang, H., and Caprioli, R.M. Capillary electrophoresis combined with matrix-assisted laser desorption ionization mass spectrometry: Continuous sample deposition on a matrix-precoated membrane target. J. Mass Spectrom. , 31, 1039-1046, 1996

Andren, P.E., and Caprioli, R.M. In vivo metabolism of substance P in rat striatum utilizing microdialysis/liquid chromatography/micro-electrospray mass spectrometry. J. Mass Spectrom., 30, 817-824, 1995

Caprioli, R.M. and Suter, M. J.-F. Mass Spectrometry. Introduction to Biophysical Methods, (Eds., J.A. Glasel and M.P. Deutscher), Academic Press Inc., Orlando, FL, 147-204, 1995

Emmett, M.R., Andren, P.E., and Caprioli, R.M. Specific molecular mass detection of endogenously released neuropeptides using in vivo microdialysis/mass spectrometry. J. Neuroscience , 62, 141-147, 1995

Farmer, T.B., and Caprioli, R.M. Mass discrimination in MALDI TOF MS: A study using crosslinked oligomeric complexes. J. Mass Spectrom., 30, 1245-1254, 1995

Lecchi, P., and Caprioli, R.M. Matrix-assisted laser desorption mass spectrometry for peptide mapping. New Methods in Peptide Mapping for the Characterization of Proteins, (Ed. W.S. Hancock), CRC Press, Inc., Boca Raton, FL, 219-240, 1995

Lin, S.-N., Slopis, J.M., Butler, I.J., and Caprioli, R.M. In vivo microdialysis and gas chromatography/mass spectrometry for studies on release of N-acetylaspartylglutamate and N-acetylaspartate in rat brain hypothalamus. J. Neurosci. Methods, 62, 199-205, 1995

Zhang, H., Andren, P.E., and Caprioli, R.M. Micro-preparation procedure for high sensitivity matrix-assisted laser desorption ionization mass spectrometry. J. Mass Spectrom., 30, 1768-1771, 1995

Andren, P.E., Emmett, M.R., and Caprioli, R.M. Micro-electrospray: Zeptomole/attomole per microliter sensitivity for peptides. J. Am. Soc. Mass Spectrom,, 5, 867-869, 1994

Andren, P.E., Lin, S.-N., and Caprioli, R.M. Microdialysis/mass spectrometry. Mass Spectrometry: Clinical and Biomedical Applications, Vol. 2 (D.M. Desiderio, ed), Plenum Publish. Corp., New York, 237-254, 1994

Andren, P.E., and Caprioli, R.M. Microdialysis/mass spectrometry of neuropeptides. Biological Mass Spectrometry: Present and Future, (Eds. T. Matsuo, R.M. Caprioli, M. Gross, and Y. Seyama), John Wiley & Sons Ltd., Sussex, UK, 355-367, 1994

Caprioli, R.M. On-line LC/MS and CE/MS. Biological Mass Spectrometry: Present and Future, (Eds. T. Matsuo, R.M. Caprioli, M.L. Gross and Y. Seyama), John Wiley & Sons Ltd., Sussex, UK, 75-100, 1994

Emmett, M.R., and Caprioli, R.M. Micro-electrospray MS: Ultra-high-sensitivity analysis of peptides and proteins. J. Am. Soc. Mass Spectrom, 54, 605-613, 1994

Caprioli, R.M., and Wu, A.H.B. Mass Spectrom. Anal. Chem. Review, 65(12), 470R-474R, 1993

Farmer, T.B., and Caprioli, R.M. Assessing the multimeric states of proteins by matrix-assisted laser desorption mass spectrometry: comparison with other biochemical methods. Techniques in Protein Chemistry IV, (Ed. R.H. Angeletti), Academic Press, Inc. San Diego, CA, 73-81, 1993

Caprioli, R.M., and Suter, M.J.-F. Continuous-flow fast atom bombardment: Recent advances and applications. Int. J. Mass Spectrom. and Ion Processes, 118/119, 449-476, 1992

Suter, M.J.-F, Moore, W.T., Farmer, T.B., Cottrell, J.S., and Caprioli, R.M. Analysis of protein-protein binding with laser desorption mass spectrometry: Can multimeric forms be studied?. Techniques in Protein Chemistry III, (Ed. R.H. Angeletti), Academic Press, Inc., Orlando, F, 447-445, 1992

Suter, M.J.-F., and Caprioli, R.M. An integral probe for capillary zone electrophoresis/continuous-flow fast atom bombardment mass spectrometry. JASMS, 30, 198-206, 1992

Farmer, T.B., and Caprioli, R.M. Assessing the multimeric states of proteins: Studies using laser desorption mass spectrometry. BMS, 20, 796-800, 1991

Suter, M.J.-F., DaGue, B.B., Lin, S-N, and Caprioli, R.M. Recent advances in LC/MS and CZE/MS for protein analysis. J. Chromatogr., 553, 101-116, 1991

Caprioli, R.M. Continuous-flow fast atom bombardment mass spectrometry. Biochem. CLin., 14, 1302-1310, 1990

Caprioli, R.M. Continuous-flow fast atom bombardment mass spectrometry: Direct analysis of polar and ionic compounds in aqueous solutions. Anal. Chem., 62, 477-485, 1990


Postdoctoral Position Available
No

Postdoctoral Position Details
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Updated Date
09/18/2009