Biomedical Research Education & Training
Faculty Member

Chiang, Chin, Ph.D.
Professor of Cell and Developmental Biology

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Phone Number: (615) 343-4922

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Chiang, Chin's picture
Academic history
B.S., State Univ of New York, Buffalo
M.S., Washington State Univ.
Ph.D., Washington State Univ.
Postdoctoral Fellow, The Johns Hopkins University School of Medicine
Research Associate, National Institutes of Health

Office Address   Mailing Address

4110 Medical Research Building 3

4114, MRB3 37232-8240

Research Specialty
Hedgehog signaling in development and cancer

Research Description
Our research focuses on the role Sonic hedgehog signaling plays in the pathogenesis of the two most common types of pediatric cancers, soft tissue sarcomas and brain tumors. We study the tumor cells-of-origin, initiation and propagation. In addition, we are also interested in identifying tumor biomarkers and compounds that inhibit their growth. We use state of the art approaches that include stem cell manipulation, molecular genetics, orthotopic grafting, proteomics, genome-wide RNA sequencing and CHIP.

Another area of interest is to understand how the complex brain circuitry is established from a rather small number of neural stem cells. We study signaling pathways that regulate brain stem cell proliferation and differentiation.

Bebesh, A.E., Fleming, J.T., Chiang, C., Carter, B.D. and Tyska, M.J.. Expression and localization of myosin-1d in the developing nervous system. Brain Res., 1440, 9, 2012

Cheng, F.Y., Huang, X., Sarangi, A., Ketova, T., Cooper, M.K., Litingtung, Y. and Chiang, C.. Widespread contribution of Gdf7 lineage to cerebellar cell types and implications for Hedgehog-driven medulloblastoma formation.. Plos One, 7, 35541, 2012

Huang, X., Dubuc, A.M., Hashizume, R., Berg, J., He, Y., Chiang, C. Cooper, M.K., Northcott, P.A., Yatlor, M.D., Barnes, M.J., Tijan, T., Chen, J., Hackett, C.S., Weiss, W.A., David James, C., Rowitch, D.H., Shuman, M.A., Jan, Y.H. and Jan L.Y. . Voltage-gated potassium channel EAG2 controls mitotic entry and tumor growth in medulloblastoma via regulating cell volume dynamics.. Genes Dev., 26, 1780, 2012

Ryan K.E. and Chiang, C.. Hedgehog Secretion and Signal Transduction in Vertebrates. J. Biol. Chem., 287, 179015, 2012

Mason, T.A., Kolobova, E., Liu, J., Roland, J.T., Chiang, C.. Darinaparsin is a multivalent chemotherapeutic which induces incomplete stress response with disruption of microtubules and Shh signaling. Plos One, 6(11), 27699, 2011

Huang, X, Liu, J, Ketova, T, Fleming, JT, Grover, VK, Cooper, MK, Litingtung, Y, Chiang, C. Transventricular delivery of Sonic hedgehog is essential to cerebellar ventricular zone development. Proc Natl Acad Sci U S A, 107(18), 8422-7, 2010

Huang, X., Ketova, T., Litingtung, Y. and Chiang, C. Isolation, Enrichment and Maintenance of Medulloblastoma Stem Cells. . J. Vis. Exp., 43, 2086, 2010

Huang, X., Sarangi, A., Ketova, T., Cooper, M. K., Litingtung, Y. and Chiang, C. . Intracranial Orthotopic Allografting of Medulloblastoma. . J. Vis. Exp., 44, 2153, 2010

Huang, X, Ketova, T, Fleming, JT, Wang, H, Dey, SK, Litingtung, Y, Chiang, C. Sonic hedgehog signaling regulates a novel epithelial progenitor domain of the hindbrain choroid plexus. Development, 2009

Oh, S, Huang, X, Liu, J, Litingtung, Y, Chiang, C. Shh and Gli3 activities are required for timely generation of motor neuron progenitors. Dev Biol, 331(2), 261-9, 2009 PMCID:2703695

Fogel, JL, Chiang, C, Huang, X, Agarwala, S. Ventral specification and perturbed boundary formation in the mouse midbrain in the absence of Hedgehog signaling. Dev Dyn, 237(5), 1359-72, 2008 PMCID:2586936

Huang, X, Goudy, SL, Ketova, T, Litingtung, Y, Chiang, C. Gli3-deficient mice exhibit cleft palate associated with abnormal tongue development. Dev Dyn, 237(10), 3079-87, 2008

Li, Y, Gordon, J, Manley, NR, Litingtung, Y, Chiang, C. Bmp4 is required for tracheal formation: A novel mouse model for tracheal agenesis. Dev Biol, 2008

Guerrero, I and Chiang, C.. A conserved mechanism of Hedgehog gradient formation by lipid modifications. Trends in Cell Biology, 17, 1-5, 2007

Huang, X, Litingtung, Y, Chiang, C. Region-specific requirement for cholesterol modification of sonic hedgehog in patterning the telencephalon and spinal cord. Development, 134(11), 2095-105, 2007

Huang, X, Litingtung, Y, Chiang, C. Ectopic sonic hedgehog signaling impairs telencephalic dorsal midline development: implication for human holoprosencephaly. Hum Mol Genet, 16(12), 1454-68, 2007 PMCID:1988863

Li, Y, Litingtung, Y, Ten Dijke, P, Chiang, C. Aberrant Bmp signaling and notochord delamination in the pathogenesis of esophageal atresia. Dev Dyn, 236(3), 746-54, 2007

Zhang, H, Lawson, WE, Polosukhin, VV, Pozzi, A, Blackwell, TS, Litingtung, Y, Chiang, C. Inhibitor of differentiation 1 promotes endothelial survival in a bleomycin model of lung injury in mice. Am J Pathol, 171(4), 1113-26, 2007 PMCID:1988863

Chiang, C. From Oligodactyly to Polydactyly:role of Shh and Gli3 in limb morphogenesis. In Hedgehog-Gli Signaling and Human Disease, Edited by A. Ruiz I Altaba . , 137-145, 2006

Li, Y, Zhang, H, Litingtung, Y, Chiang, C. Cholesterol modification restricts the spread of Shh gradient in the limb bud. Proc Natl Acad Sci U S A, 103(17), 6548-53, 2006 PMCID:1458921

Zhang, H., Litingtung, Y. and Chiang, C.. Sonic Hedgehog signaling in viceral organ development. In Shh and Gli signaling in Development. Edited by S. Howie and C. Fisher. , 2006

Oh, Saeock, Huang, Xi, Chiang, Chin. Specific requirements of sonic hedgehog signaling during oligodendrocyte development. Dev Dyn, 234(3), 489-96, 2005

van Bokhoven, H, Celli, J, van Reeuwijk, J, Rinne, T, Glaudemans, B, van Beusekom, E, Rieu, P, Newbury-Ecob, RA, Chiang, C, Brunner, HG. MYCN haploinsufficiency is associated with reduced brain size and intestinal atresias in Feingold syndrome. Nat Genet, 37(5), 465-7, 2005

Li, Yina, Zhang, Huimin, Choi, Seung Cheol, Litingtung, Ying, Chiang, Chin. Sonic hedgehog signaling regulates Gli3 processing, mesenchymal proliferation, and differentiation during mouse lung organogenesis. Dev Biol, 270(1), 214-31, 2004

Powles, N, Marshall, H, Economou, A, Chiang, C, Murakami, A, Dickson, C, Krumlauf, R, Maconochie, M. Regulatory analysis of the mouse Fgf3 gene: control of embryonic expression patterns and dependence upon sonic hedgehog (Shh) signalling. Dev Dyn, 230(1), 44-56, 2004

Chiang, CW, Kanies, C, Kim, KW, Fang, WB, Parkhurst, C, Xie, M, Henry, T, Yang, E. Protein phosphatase 2A dephosphorylation of phosphoserine 112 plays the gatekeeper role for BAD-mediated apoptosis. Mol Cell Biol, 23(18), 6350-62, 2003 PMCID:193703

Litingtung, Ying, Dahn, Randall D, Li, Yina, Fallon, John F, Chiang, Chin. Shh and Gli3 are dispensable for limb skeleton formation but regulate digit number and identity. Nature, 418(6901), 979-83, 2002

Liu, W, Li, G, Chien, JS, Raft, S, Zhang, H, Chiang, C, Frenz, DA. Sonic hedgehog regulates otic capsule chondrogenesis and inner ear development in the mouse embryo. Dev Biol, 248(2), 240-50, 2002

Ohkubo, Y, Chiang, C, Rubenstein, JL. Coordinate regulation and synergistic actions of BMP4, SHH and FGF8 in the rostral prosencephalon regulate morphogenesis of the telencephalic and optic vesicles. Neuroscience, 111(1), 1-17, 2002

Perriton, CL, Powles, N, Chiang, C, Maconochie, MK, Cohn, MJ. Sonic hedgehog signaling from the urethral epithelium controls external genital development. Dev Biol, 247(1), 26-46, 2002

Chattopadhyay, A, Chiang, CW, Yang, E. BAD/BCL-[X(L)] heterodimerization leads to bypass of G0/G1 arrest. Oncogene, 20(33), 4507-18, 2001

Chiang, C, Litingtung, Y, Harris, M P, Simandl, B K, Li, Y, Beachy, P A, Fallon, J F. Manifestation of the limb prepattern: limb development in the absence of sonic hedgehog function. Dev Biol, 236(2), 421-35, 2001

Chiang, CW, Harris, G, Ellig, C, Masters, SC, Subramanian, R, Shenolikar, S, Wadzinski, BE, Yang, E. Protein phosphatase 2A activates the proapoptotic function of BAD in interleukin- 3-dependent lymphoid cells by a mechanism requiring 14-3-3 dissociation. Blood, 97(5), 1289-97, 2001

Mo, R, Kim, JH, Zhang, J, Chiang, C, Hui, CC, Kim, PC. Anorectal malformations caused by defects in sonic hedgehog signaling. Am J Pathol, 159(2), 765-74, 2001 PMCID:1850556

Litingtung, Y, Chiang, C. Control of Shh activity and signaling in the neural tube. Dev Dyn, 219(2), 143-54, 2000

Litingtung, Y, Chiang, C. Specification of ventral neuron types is mediated by an antagonistic interaction between Shh and Gli3. Nat Neurosci, 3(10), 979-85, 2000

Pak, JH, Huang, FL, Li, J, Balschun, D, Reymann, KG, Chiang, C, Westphal, H, Huang, KP. Involvement of neurogranin in the modulation of calcium/calmodulin-dependent protein kinase II, synaptic plasticity, and spatial learning: a study with knockout mice. Proc Natl Acad Sci U S A, 97(21), 11232-7, 2000 PMCID:17183

Borycki, AG, Brunk, B, Tajbakhsh, S, Buckingham, M, Chiang, C, Emerson, CP. Sonic hedgehog controls epaxial muscle determination through Myf5 activation. Development, 126(18), 4053-63, 1999

Chiang, C, Swan, RZ, Grachtchouk, M, Bolinger, M, Litingtung, Y, Robertson, EK, Cooper, MK, Gaffield, W, Westphal, H, Beachy, PA, Dlugosz, AA. Essential role for Sonic hedgehog during hair follicle morphogenesis. Dev Biol, 205(1), 1-9, 1999

Pierani, A, Brenner-Morton, S, Chiang, C, Jessell, TM. A sonic hedgehog-independent, retinoid-activated pathway of neurogenesis in the ventral spinal cord. Cell, 97(7), 903-15, 1999

Kos, L, Chiang, C, Mahon, KA. Mediolateral patterning of somites: multiple axial signals, including Sonic hedgehog, regulate Nkx-3.1 expression. Mech Dev, 70(1-2), 25-34, 1998

Litingtung, Y, Lei, L, Westphal, H, Chiang, C. Sonic hedgehog is essential to foregut development. Nat Genet, 20(1), 58-61, 1998

Knezevic, V., De Santo, R., Schughart, K., Huffstadt, U., Chiang, C., Mahon, K.A. and Mackem, S. Hoxd-12 differentially affects preaxial and postaxial chondrogenic branches in the limb and regulates sonic hedgehog in a positive feedback loop. Development, 124, 4523-4536, 1997

Chiang C, Litingtung Y, Lee E, Young K, Corden JL, Westphal H and Beachy PA. Cyclopia and defective axial patterning in mice lacking Sonic Hedgehog gene function. Nature, 383, 407-413, 1996

Yuryev, A, Patturajan, M, Litingtung, Y, Joshi, RV, Gentile, C, Gebara, M, Corden, JL. The C-terminal domain of the largest subunit of RNA polymerase II interacts with a novel set of serine/arginine-rich proteins. Proc Natl Acad Sci U S A, 93(14), 6975-80, 1996 PMCID:38919

Chiang, C., Yong, K.E., and Beachy, P.A.. Controlof drosphila trachael branching by the novel homeodomain gene unplugges, a regulatory target for genes of the bithorax complex.. Development, 121, 3901-3912, 1995

Fan, C.-M., Porter, J.A., Chiang, C., Chang, D.T., Beachy, P.A., Tessier,-Lavigne, M.. Long range sclerotome induction by Sonic Hedgehog: direct role of the amino-terminal clleavage product and modulation by the cyclic AMP signalling pathway. Cell, 81, 457-465, 1995

Hynes, M., Porter, J.A., Chiang, C, Chang, D., Tessier-Lavigne, M., Beachy P.A., and Rosenthal, A.. Induction of midbrain dopaminergic neurons product of hedgehog autoproteolysis. Neuron, 15, 35-44, 1995

Lopez, A., Chang, D.T., Chiang, C., Porter, J., maria, A.R., Simandl, K., Beachy, P.A., and Fallon, J.F.. Limb patterning activity and restricted posterior localization of the amino terminal product of hedgehog autoproteolysis. Current Biology, 5, 791-796, 1995

Roelink, H., Porter, J., Chiang, C., Tanabe, Y., Chang, D.T., Beachy, P.A., and Jessell, T.M.. Concentration-dependent induction of floor plate and motor neuron differentiation by the amino terminal autoproteolytic fragment of Sonic Hedgehog.. Cell, 81, 445-455, 1995

Chang, D.T., Lopez, A., von Kessler, D.P., Chiang, C., Simandl, K., Renbin, Z., Seldin, M.F., Fallon, J.F., and Beachy, P.A. Products, genetic linkage, and limb patterning activity of a murine hedgehog gene.. Development, 120, 3339-3353, 1994

Chiang, C., andBeachy, P.A. Expression of a novel Toll-like gene spans the parasegment boundary and contributes to hedgehog function in the adult eye of Drosophila. Mech. Dev., 47, 225-239, 1994

Chiang, C.,, Patel, N., Young, K., and Beachy, P.A.. The novel homeodomain gene buttonless specifies differentiation and axonal guidance functions of Drosophila dorsal median cells. Development, 120, 3581-3593, 1994

Postdoctoral Position Available

Postdoctoral Position Details
Contact Chin Chiang at for more details.

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