Vanderbilt University School of
514 RRB 6602
lipid signaling, lipid hormones, cyclooxygenase, lipoxygenase, mechanism, chemoprevention, structure-function, prostaglandin, leukotriene, curcumin, stereochemistry, lipid peroxidation, subcellular localization, structural biology, biochemistry, enzyme action, mass spectrometry, mutation, NMR, protein structure, spectroscopy, structural biology
Discovery and characterization of novel lipid mediators. Mechanisms of oxygenation of fatty acids. Chemopreventive activity of curcumin.
We are studying the biochemistry of lipid signaling. We are interested in how lipid signaling hormones are formed and what their roles are in human health and disease.
1. Discovery and characterization of novel lipid mediators
The cyclooxygenase and lipoxygenase enzymes catalyze the first step in the formation of a diverse array of lipid mediators (?eicosanoids?) from arachidonic acid. We are interested in exploring novel pathways of how these mediators are formed, and to establish their role in the progression of inflammatory diseases like atherosclerosis or cancer.
2. Mechanisms of oxygenation of fatty acids
Lipid peroxidation and oxidative stress are elementary pathophysiological processes involved in the progression of many diseases, e.g., atherosclerosis, Alzheimer's, and cardiovascular diseases. The lipid aldehyde, 4-hydroxynonenal (HNE), has been recognized as one of the most cytotoxic products formed during lipid peroxidation almost three decades ago. Yet despite the wealth of information on its pathological properties, only little is known about its mechanism(s) of formation. We are using model autoxidation reactions with common and unusual fatty acid hydroperoxide precursors to identify mechanistically significant intermediates that shed a light on the pathways of formation of HNE. The products are analyzed using LC-MS, NMR, GC-MS, UV, and CD spectroscopy. Part of this research is carried out in collaboration with Ned Porters group in the Department of Chemistry.
3. Curcumin as a chemopreventive agent
Curcumin is the spice that gives curry its yellow color. It has also been used in traditional Asian medicine as an anti-inflammatory and wound-healing agent. In the past few years, curcumin has received increasing attention for use as a dietary cancer chemopreventive agent. We are studying the oxidative metabolism of curcumin, and we are testing the hypothesis that oxidative metabolites of curcumin are contributing to its chemopreventive activity.
Orlando, BJ, McDougle, DR, Lucido, MJ, Eng, ET, Graham, LA, Schneider, C, Stokes, DL, Das, A, Malkowski, MG. Cyclooxygenase-2 catalysis and inhibition in lipid bilayer nanodiscs. Arch Biochem Biophys, , , 2014.
Brash, AR, Boeglin, WE, Stec, DF, Voehler, M, Schneider, C, Cha, JK. Isolation and characterization of two geometric allene oxide isomers synthesized from 9S-hydroperoxylinoleic acid by cytochrome P450 CYP74C3: stereochemical assignment of natural fatty acid allene oxides. J Biol Chem, 288(29), 20797-806, 2013.
Gordon, ON, Graham, LA, Schneider, C. Facile synthesis of deuterated and [(14) C]labeled analogs of vanillin and curcumin for use as mechanistic and analytical tools. J Labelled Comp Radiopharm, 56(14), 696-9, 2013.
Ketron, AC, Gordon, ON, Schneider, C, Osheroff, N. Oxidative Metabolites of Curcumin Poison Human Type II Topoisomerases. Biochemistry, 52(1), 221-7, 2013.
Brash, AR, Schneider, C, Hamberg, M. Applications of Stereospecifically-Labeled Fatty Acids in Oxygenase and Desaturase Biochemistry. Lipids, 47, 101-116, 2012. PMCID:3243484
Gordon, ON, Schneider, C. Vanillin and ferulic acid: not the major degradation products of curcumin. Trends Molec. Med., 18, 361-363, 2012.
McElroy, SJ, Hobbs, S, Kallen, M, Tejera, N, Rosen, MJ, Grishin, A, Matta, P, Schneider, C, Upperman, J, Ford, H, Polk, DB, Weitkamp, JH. Transactivation of EGFR by LPS induces COX-2 expression in enterocytes. PLoS One, 7(5), e38373, 2012.
Tejera, N, Boeglin, WE, Suzuki, T, Schneider, C. COX-2-dependent and -independent biosynthesis of dihydroxy-arachidonic acids in activated human leukocytes. J Lipid Res, 53(1), 87-94, 2012. PMCID:3243484
Griesser, M, Pistis, V, Suzuki, T, Tejera, N, Pratt, DA, Schneider, C. Autoxidative and cyclooxygenase-2 catalyzed transformation of the dietary chemopreventive agent curcumin. J Biol Chem, 286(2), 1114-24, 2011.
Griesser, M, Suzuki, T, Tejera, N, Mont, S, Boeglin, WE, Pozzi, A, Schneider, C. Biosynthesis of hemiketal eicosanoids by cross-over of the 5-lipoxygenase and cyclooxygenase-2 pathways. Proc Natl Acad Sci U S A, 108(17), 6945-50, 2011.
Lee, SH, Schneider, C, Higdon, AN, Darley-Usmar, VM, Chung, CY. Role of iPLA(2) in the Regulation of Src Trafficking and Microglia Chemotaxis. Traffic, , , 2011.
Liu, W, Porter, NA, Schneider, C, Brash, AR, Yin, H. Formation of 4-hydroxynonenal from cardiolipin oxidation: Intramolecular peroxyl radical addition and decomposition. Free Radic Biol Med, 50(1), 166-78, 2011.
Schneider, C, Pozzi, A. Cyclooxygenases and lipoxygenases in cancer. Cancer Metastasis Rev, 30(3-4), 277-94, 2011. PMCID:3243484
Mulugeta, S, Suzuki, T, Tejera Hernandez, N, Griesser, M, Boeglin, WE, Schneider, C. Identification and absolute configuration of dihydroxy-arachidonic acids formed by oxygenation of 5S-HETE by native and aspirin-acetylated COX-2. J Lipid Res, 51(3), 575-585, 2010.
Gao, B, Boeglin, WE, Zheng, Y, Schneider, C, Brash, AR. Evidence for an ionic intermediate in the transformation of fatty acid hydroperoxide by a catalase-related allene oxide synthase from the Cyanobacterium Acaryochloris marina. J Biol Chem, 284(33), 22087-98, 2009.
Griesser, M, Boeglin, WE, Suzuki, T, Schneider, C. Convergence of the 5-LOX and COX-2 pathways: heme-catalyzed cleavage of the 5S-HETE-derived di-endoperoxide into aldehyde fragments. J Lipid Res, 50(12), 2455-62, 2009.
Niisuke, K, Boeglin, WE, Murray, JJ, Schneider, C, Brash, AR. Biosynthesis of a linoleic acid allylic epoxide: mechanistic comparison with its chemical synthesis and leukotriene A biosynthesis. J Lipid Res, 50(7), 1448-55, 2009. PMCID:2694342
Schneider, C. An update on products and mechanisms of lipid peroxidation. Mol Nutr Food Res, 53(3), 315-21, 2009.
Schneider, C. Books: Nutrition and Arthritis. Edited by Margaret Rayman and Alison Callaghan. Mol Nutr Food Res, 52(4), 502, 2008.
Schneider, C, Boeglin, WE, Yin, H, Porter, NA, Brash, AR. Intermolecular Peroxyl Radical Reactions during Autoxidation of Hydroxy and Hydroperoxy Arachidonic Acids Generate a Novel Series of Epoxidized Products. Chem Res Toxicol, 21(4), 895-903, 2008.
Schneider, C, Porter, NA, Brash, AR. Routes to 4-hydroxynonenal: Fundamental issues in the mechanisms of lipid peroxidation. J Biol Chem, 283(23), 15539-15543, 2008. PMCID:2414272
Zheng, Y, Boeglin, WE, Schneider, C, Brash, AR. A 49-kDa mini-lipoxygenase from Anabaena sp. PCC 7120 retains catalytically complete functionality. J Biol Chem, 283(8), 5138-47, 2008.
Brash, AR, Yu, Z, Boeglin, WE, Schneider, C. The hepoxilin connection in the epidermis. FEBS J, 274(14), 3494-502, 2007.
Schneider, C. Book news: stress, obesity, and metabolic syndrome. Edited by George P. Chrousos and Constantine Tsigos. Mol Nutr Food Res, 51(12), 1554, 2007.
Schneider, C, Niisuke, K, Boeglin, WE, Voehler, M, Stec, DF, Porter, NA, Brash, AR. Enzymatic synthesis of a bicyclobutane fatty acid by a hemoprotein lipoxygenase fusion protein from the cyanobacterium Anabaena PCC 7120. Proc Natl Acad Sci U S A, 104(48), 18941-5, 2007. PMCID:2141887
Schneider, C, Pratt, DA, Porter, NA, Brash, AR. Control of oxygenation in lipoxygenase and cyclooxygenase catalysis. Chem Biol, 14(5), 473-88, 2007. PMCID:2692746
Schneider, C, Yu, Z, Boeglin, WE, Zheng, Y, Brash, AR. Enantiomeric separation of hydroxy and hydroperoxy eicosanoids by chiral column chromatography. Methods Enzymol, 433, 145-57, 2007.
Yu, Z, Schneider, C, Boeglin, WE, Brash, AR. Epidermal Lipoxygenase Products of the Hepoxilin Pathway Selectively Activate the Nuclear Receptor PPARalpha. Lipids, 42(6), 491-7, 2007.
Furse, KE, Pratt, DA, Schneider, C, Brash, AR, Porter, NA, Lybrand, TP. Molecular Dynamics Simulations of Arachidonic Acid-Derived Pentadienyl Radical
Intermediate Complexes with COX-1 and COX-2: Insights into Oxygenation Regio- and Stereoselectivity. Biochemistry, 45(10), 3206-18, 2006. PMCID:2504531
Schneider, C, Boeglin, WE, Yin, H, Stec, DF, Voehler, M. Convergent oxygenation of arachidonic acid by 5-lipoxygenase and cyclooxygenase-2. J Am Chem Soc, 128(3), 720-1, 2006. PMCID:2532595
Yu, Z, Schneider, C, Boeglin, WE, Brash, AR. Human and mouse eLOX3 have distinct substrate specificities: implications for their linkage with lipoxygenases in skin. Arch Biochem Biophys, 455(2), 188-96, 2006. PMCID:2636205
Chang, MS, Schneider, C, Roberts, RL, Shappell, SB, Haselton, FR, Boeglin, WE, Brash, AR. Detection and subcellular localization of two 15S-lipoxygenases in human cornea. Invest Ophthalmol Vis Sci, 46(3), 849-56, 2005.
Coffa, G, Imber, AN, Maguire, BC, Laxmikanthan, G, Schneider, C, Gaffney, BJ, Brash, AR. On the Relationships of Substrate Orientation, Hydrogen Abstraction, and Product
Stereochemistry in Single and Double Dioxygenations by Soybean Lipoxygenase-1 and Its Ala542Gly Mutant. J Biol Chem, 280(46), 38756-38766, 2005. PMCID:1351262
Coffa, G, Schneider, C, Brash, AR. A comprehensive model of positional and stereo control in lipoxygenases. Biochem Biophys Res Commun, 338(1), 87-92, 2005.
Schneider, C, Boeglin, WE, Yin, H, Ste, DF, Hachey, DL, Porter, NA, Brash, AR. Synthesis of dihydroperoxides of linoleic and linolenic acids and studies on
their transformation to 4-hydroperoxynonenal. Lipids, 40(11), 1155-62, 2005.
Schneider, Claus. Chemistry and biology of vitamin E. Mol Nutr Food Res, 49(1), 7-30, 2005.
Schneider, Claus, Boeglin, William E, Brash, Alan R. Human cyclo-oxygenase-1 and an alternative splice variant: contrasts in expression
of mRNA, protein and catalytic activities. Biochem J, 385(Pt 1), 57-64, 2005. PMCID:1134673
Yu, Zheyong, Schneider, Claus, Boeglin, William E, Brash, Alan R. Mutations associated with a congenital form of ichthyosis (NCIE) inactivate
the epidermal lipoxygenases 12R-LOX and eLOX3. Biochim Biophys Acta, 1686 3, 238-47, 2005.
Schneider, Claus, Boeglin, William E, Brash, Alan R. Identification of two cyclooxygenase active site residues, Leucine 384 and Glycine 526, that control carbon ring cyclization in prostaglandin biosynthesis. J Biol Chem, 279(6), 4404-14, 2004.
Schneider, Claus, Porter, Ned A, Brash, Alan R. Autoxidative transformation of chiral omega6 hydroxy linoleic and arachidonic acids to chiral 4-hydroxy-2E-nonenal. Chem Res Toxicol, 17(7), 937-41, 2004.
Schneider, Claus, Strayhorn, W David, Brantley, Dana M, Nanney, Lillian B, Yull, Fiona E, Brash, Alan R. Upregulation of 8-lipoxygenase in the dermatitis of IkappaB-alpha-deficient mice. J Invest Dermatol, 122(3), 691-8, 2004.
Valmsen, Karin, Boeglin, William E, Jarving, Ivar, Schneider, Claus, Varvas, Kulliki, Brash, Alan R, Samel, Nigulas. Structural and functional comparison of 15S- and 15R-specific cyclooxygenases from the coral Plexaura homomalla. Eur J Biochem, 271(17), 3533-8, 2004.
West, James D, Ji, Chuan, Duncan, Stephen T, Amarnath, Venkataraman, Schneider, Claus, Rizzo, Carmelo J, Brash, Alan R, Marnett, Lawrence J. Induction of Apoptosis in Colorectal Carcinoma Cells Treated with 4-Hydroxy-2-nonenal and Structurally Related Aldehydic Products of Lipid Peroxidation. Chem Res Toxicol, 17(4), 453-62, 2004.
Brash, Alan R., Jisaka, Mitsuo, Boeglin, William E., Schneider, Claus. On the basis for the positional specificity and stereo specificity of lipoxygenases. Int. Congress Series, 1233, 297-305, 2003.
Yu, Zheyong, Schneider, Claus, Boeglin, William E, Marnett, Lawrence J, Brash, Alan R. The lipoxygenase gene ALOXE3 implicated in skin differentiation encodes a hydroperoxide isomerase. Proc Natl Acad Sci U S A, 100(16), 9162-7, 2003. PMCID:170889
Schneider, C., Boeglin, W.E., Brash, A.R. Analysis of cyclooxygenase-substrate interactions using stereospecifically-labeled arachidonic acids. Adv Exp Med Biol, 507, 49-53, 2002.
Schneider, Claus, Boeglin, William E, Prusakiewicz, Jeffery J, Rowlinson, Scott W, Marnett, Lawrence J, Samel, Nigulas, Brash, Alan R. Control of prostaglandin stereochemistry at the 15-carbon by cyclooxygenases-1 and -2. A critical role for serine 530 and valine 349. J Biol Chem, 277(1), 478-85, 2002.
Schneider, Claus, Brash, Alan R. Lipoxygenase-catalyzed formation of R-configuration hydroperoxides. Prostaglandins Other Lipid Mediat, 68-69, 291-301, 2002.
Schneider, Claus, Manier, M Lisa, Hachey, David L, Brash, Alan R. Detection of the 15-acetate of prostaglandin E2 methyl ester as a prominent component of the prostaglandins in the gorgonian coral Plexaura homomalla. Lipids, 37(2), 217-21, 2002.
Kozak, K R, Prusakiewicz, J J, Rowlinson, S W, Schneider, C, Marnett, L J. Amino acid determinants in cyclooxygenase-2 oxygenation of the endocannabinoid 2-arachidonylglycerol. J Biol Chem, 276(32), 30072-7, 2001.
Schneider, C, Keeney, D S, Boeglin, W E, Brash, A R. Detection and cellular localization of 12R-lipoxygenase in human tonsils. Arch Biochem Biophys, 386(2), 268-74, 2001.
Schneider, C, Tallman, K A, Porter, N A, Brash, A R. Two distinct pathways of formation of 4-hydroxynonenal. Mechanisms of nonenzymatic transformation of the 9- and 13-hydroperoxides of linoleic acid to 4-hydroxyalkenals. J Biol Chem, 276(24), 20831-8, 2001.
Shappell, S B, Gupta, R A, Manning, S, Whitehead, R, Boeglin, W E, Schneider, C, Case, T, Price, J, Jack, G S, Wheeler, T M, Matusik, R J, Brash, A R, Dubois, R N. 15S-Hydroxyeicosatetraenoic acid activates peroxisome proliferator-activated receptor gamma and inhibits proliferation in PC3 prostate carcinoma cells. Cancer Res, 61(2), 497-503, 2001.
Tijet, N, Schneider, C, Muller, B L, Brash, A R. Biogenesis of volatile aldehydes from fatty acid hydroperoxides: molecular cloning of a hydroperoxide lyase (CYP74C) with specificity for both the 9- and 13-hydroperoxides of linoleic and linolenic acids. Arch Biochem Biophys, 386(2), 281-9, 2001.
Rowlinson, S W, Crews, B C, Goodwin, D C, Schneider, C, Gierse, J K, Marnett, L J. Spatial requirements for 15-(R)-hydroxy-5Z,8Z,11Z, 13E-eicosatetraenoic acid synthesis within the cyclooxygenase active site of murine COX-2. Why acetylated COX-1 does not synthesize 15-(R)-hete. J Biol Chem, 275(9), 6586-91, 2000.
Schneider, C, Boeglin, W E, Brash, A R. Enantiomeric separation of hydroxy eicosanoids by chiral column chromatography: effect of the alcohol modifier. Anal Biochem, 287(1), 186-9, 2000.
Schneider, C, Boeglin, W E, Lai, S, Cha, J K, Brash, A R. Synthesis and applications of stereospecifically (3)H-labeled arachidonic acids as mechanistic probes for lipoxygenase and cyclooxygenase catalysis. Anal Biochem, 284(1), 125-35, 2000.
Schneider, C, Brash, A R. Stereospecificity of hydrogen abstraction in the conversion of arachidonic acid to 15R-HETE by aspirin-treated cyclooxygenase-2. Implications for the alignment of substrate in the active site. J Biol Chem, 275(7), 4743-6, 2000.
Schneider, C. and Schreier, P. Catalytic properties of allene oxide synthase from flaxseed (Linum usitatissimum L.). Lipids, 33, 191-196, 1998.
Schneider, C., Amberg, A., Feurle, J., Ross, A., Roth, M., Toth, G., and Schreier, P. 2-[4'-Hydroxy-3'-methoxy)-phenoxy]-4-(4"-hydroxy-3"-methoxy-phenyl)-8-hydroxy-6-oxo-3-oxabicyclo[3.3.0]-7-octene: Unusual product of the soybean lipoxygenase-catalyzed oxygenation of curcumin. J. Mol. Catal. B: Enzymatic, 4, 219-227, 1998.
Herderich, M., Richling, E., Roscher, R., Schneider, C., Schwab, W., Humpf, H.-U., and Schreier, P. Application of atmospheric pressure ionization HPLC-MS-MS for the analysis of natural products. Chromatographia, 45, 127-132, 1997.
Schneider, C., Schreier, P., and Herderich, M. Analysis of lipoxygenase-derived fatty acid hydroperoxides by electrospray ionization tandem mass spectrometry. Lipids, 32, 331-336, 1997.
Schneider, C., Schreier, P., and Humpf, H.-U. Exciton-coupled circular dichroism (ECCD) in acyclic hydroxylated dienes: A sensitive method for the direct stereochemical assignment of lipoxygenase products. Chirality, 9, 563-567, 1997.
Schneider, C., Wein, M., Harmsen, D., and Schreier, P. A fatty acid alpha-ketol, a product of the plant lipoxygenase pathway, is oxidized to 3(Z)-dodecendioic acid by a bacterial monooxygenase. Biochem. Biophys. Res. Commun., 232, 364-366, 1997.
NIH-funded positions available for a start date of October 1, 2013. Candidates should have experience in structural analysis of small molecules using HPLC, NMR, LC-MS. A background in lipid or natural product biochemistry would be a big plus. We also welcome candidates that, in addition, are proficient in cell culture and molecular biology.
Our laboratory encourages international students to apply for post-graduate research. In particular, we are interested in recruiting Brazilian Science without Borders fellows. For information please contact the PI.
Vanderbilt University is committed to principles of equal opportunity and affirmative action.
Copyright © 2008 Vanderbilt University School of Medicine
The office of Biomedical Research Education & Training All rights reserved.
For questions or problems concerning this page, please submit a help ticket.