Vanderbilt University School of Medicine

Macdonald, Robert L. , M.D., Ph.D.
Chair of the Department of Neurology
Professor of Neurology
Professor of Pharmacology
Gerald M. Fenichel Chair in Neurology
Professor of Molecular Physiology and Biophysics

Lab Url: http://www.mc.vanderbilt.edu/neurology/

Phone Number: (615) 936-2287

Email Address:robert.macdonald@vanderbilt.edu

Macdonald, Robert's picture

Office Address   Mailing Address

6140 MRB III

A 1123A Medical Center North 37232-3375


Research Keywords
neuroscience, pharmacology, physiology, electrophysiolgy, membrane biophysics, epilepsy research, GABAA receptors, Ion channels, Kinase, Membrane, Molecular, medicine, Mutation, Phosphorylation, Polymorphism, Post-translational modification ,Receptor,Transcription,Translation

Research Specialty
Transcription, translation, folding, assembly, trafficking and pharmacological and electrophysiological properties of recombinant/native GABAA receptor channels and of mutant GABAA receptor channels associated with genetic epilepsy syndromes in transfected HEK293T cells and in transgenic mice.

Research Description
Transcription, translation, folding, assembly, trafficking and pharmacological and electrophysiological properties of recombinant/native GABAA receptor channels and of mutant GABAA receptor channels associated with genetic epilepsy syndromes in transfected HEK293T cells and in transgenic mice.

Clinical Interests
We are interested in the basic mechanisms of Epilepsy and of the mechanisms of action of antiepileptic drugs.

Publications
Botzolakis, EJ, Zhao, J, Gurba, KN, Macdonald, RL, Hedera, P. The effect of HSP-causing mutations in SPG3A and NIPA1 on the assembly, trafficking, and interaction between atlastin-1 and NIPA1. Mol Cell Neurosci, 46(1), 122-35, 2011.

Delahanty, RJ, Kang, JQ, Brune, CW, Kistner, EO, Courchesne, E, Cox, NJ, Cook, EH, Macdonald, RL, Sutcliffe, JS. Maternal transmission of a rare GABRB3 signal peptide variant is associated with autism. Mol Psychiatry, 16(1), 86-96, 2011. PMCID:2822691

Ding, L, Feng, HJ, Macdonald, RL, Botzolakis, EJ, Hu, N, Gallagher, MJ. GABA(A) receptor alpha1 subunit mutation A322D associated with autosomal dominant juvenile myoclonic epilepsy reduces the expression and alters the composition of wild type GABA(A) receptors. J Biol Chem, 285(34), 26390-405, 2010.

Feng, HJ, Kao, C, Gallagher, MJ, Jansen, ED, Mahadevan-Jansen, A, Konrad, PE, Macdonald, RL. Alteration of GABAergic neurotransmission by pulsed infrared laser stimulation. J Neurosci Methods, 192(1), 110-4, 2010.

Feng, HJ, Macdonald, RL. Barbiturates require the N terminus and first transmembrane domain of the delta subunit for enhancement of alpha1beta3delta GABAA receptor currents. J Biol Chem, 285(31), 23614-21, 2010.

Kang, JQ, Shen, W, Lee, M, Gallagher, MJ, Macdonald, RL. Slow degradation and aggregation in vitro of mutant GABAA receptor gamma2(Q351X) subunits associated with epilepsy. J Neurosci, 30(41), 13895-905, 2010.

Lo, WY, Lagrange, AH, Hernandez, CC, Harrison, R, Dell, A, Haslam, SM, Sheehan, JH, Macdonald, RL. Glycosylation of {beta}2 subunits regulates GABAA receptor biogenesis and channel gating. J Biol Chem, 285(41), 31348-61, 2010.

Macdonald, RL, Kang, JQ, Gallagher, MJ. Mutations in GABAA receptor subunits associated with genetic epilepsies. J Physiol, 588(Pt 11), 1861-9, 2010.

Tang, X, Hernandez, CC, Macdonald, RL. Modulation of spontaneous and GABA-evoked tonic alpha4beta3delta and alpha4beta3gamma2L GABAA receptor currents by protein kinase A. J Neurophysiol, 103(2), 1007-19, 2010. PMCID:2822691

Botzolakis, EJ, Maheshwari, A, Feng, HJ, Lagrange, AH, Shaver, JH, Kassebaum, NJ, Venkataraman, R, Baudenbacher, F, Macdonald, RL. Achieving synaptically relevant pulses of neurotransmitter using PDMS microfluidics. J Neurosci Methods, 177(2), 294-302, 2009. PMCID:2660329

Feng, HJ, Botzolakis, EJ, Macdonald, RL. Context-dependent modulation of alphabetagamma and alphabetadelta GABA A receptors by penicillin: implications for phasic and tonic inhibition. Neuropharmacology, 56(1), 161-73, 2009. PMCID:2661208

Kang, JQ, Macdonald, RL. Making sense of nonsense GABA(A) receptor mutations associated with genetic epilepsies. Trends Mol Med, 15(9), 430-8, 2009. PMCID:2822691

Kang, JQ, Shen, W, Macdonald, RL. The GABRG2 mutation, Q351X, associated with generalized epilepsy with febrile seizures plus, has both loss of function and dominant-negative suppression. J Neurosci, 29(9), 2845-56, 2009. PMCID:2754234

Kang, JQ, Shen, W, Macdonald, RL. Two molecular pathways (NMD and ERAD) contribute to a genetic epilepsy associated with the GABA(A) receptor GABRA1 PTC mutation, 975delC, S326fs328X. J Neurosci, 29(9), 2833-44, 2009. PMCID:2687144

Macdonald, RL, Kang, JQ. Molecular pathology of genetic epilepsies associated with GABAA receptor subunit mutations. Epilepsy Curr, 9(1), 18-23, 2009. PMCID:2668111

Feng, HJ, Mathews, GC, Kao, C, Macdonald, RL. Alterations of GABA A-receptor function and allosteric modulation during development of status epilepticus. J Neurophysiol, 99(3), 1285-93, 2008.

Lo, WY, Botzolakis, EJ, Tang, X, Macdonald, RL. A conserved Cys-loop receptor aspartate residue in the M3-M4 cytoplasmic loop is required for GABAA receptor assembly. J Biol Chem, 283(44), 29740-52, 2008. PMCID:2573071

Rula, EY, Lagrange, AH, Jacobs, MM, Hu, N, Macdonald, RL, Emeson, RB. Developmental modulation of GABA(A) receptor function by RNA editing. J Neurosci, 28(24), 6196-201, 2008. PMCID:2746000

Zhao, J, Matthies, DS, Botzolakis, EJ, Macdonald, RL, Blakely, RD, Hedera, P. Hereditary spastic paraplegia-associated mutations in the NIPA1 gene and its Caenorhabditis elegans homolog trigger neural degeneration in vitro and in vivo through a gain-of-function mechanism. J Neurosci, 28(51), 13938-51, 2008. PMCID:2660329

Gallagher, MJ, Ding, L, Maheshwari, A, Macdonald, RL. The GABAA receptor alpha1 subunit epilepsy mutation A322D inhibits transmembrane helix formation and causes proteasomal degradation. Proc Natl Acad Sci U S A, 104(32), 12999-3004, 2007. PMCID:1941799

Lagrange, AH, Botzolakis, EJ, Macdonald, RL. Enhanced macroscopic desensitization shapes the response of alpha4 subtype-containing GABAA receptors to synaptic and extrasynaptic GABA. J Physiol, 578(Pt 3), 655-76, 2007. PMCID:2151343

Audenaert, D, Schwartz, E, Claeys, KG, Claes, L, Deprez, L, Suls, A, Van Dyck, T, Lagae, L, Van Broeckhoven, C, Macdonald, RL, De Jonghe, P. A novel GABRG2 mutation associated with febrile seizures. Neurology, 67(4), 687-90, 2006.

Feng, HJ, Kang, JQ, Song, L, Dibbens, L, Mulley, J, Macdonald, RL. Delta subunit susceptibility variants E177A and R220H associated with complex epilepsy alter channel gating and surface expression of alpha4beta2delta GABAA receptors. J Neurosci, 26(5), 1499-506, 2006.

Kang, JQ, Shen, W, Macdonald, RL. Why does fever trigger febrile seizures? GABAA receptor gamma2 subunit mutations associated with idiopathic generalized epilepsies have temperature-dependent trafficking deficiencies. J Neurosci, 26(9), 2590-7, 2006.

Macdonald, RL, Kang, JQ, Gallagher, MJ, Feng, HJ. GABA(A) receptor mutations associated with generalized epilepsies. Adv Pharmacol, 54, 147-69, 2006.

Gallagher, MJ, Shen, W, Song, L, Macdonald, RL. Endoplasmic reticulum retention and associated degradation of a GABAA receptor epilepsy mutation that inserts an aspartate in the M3 transmembrane segment of the alpha1 subunit. J Biol Chem, 280(45), 37995-8004, 2005.

Jones-Davis DM, Song L, Macdonald RL:. Structural determinants of benzodiazepine allosteric regulation of GABAA receptor currents. . J Neurosci, 25, 8056-8065, 2005.

Dibbens LM, Hua-Jun Feng H-J, Richards MC, Harkin LA, Hodgson BL, Scott D, Jenkins M, Petrou S, Sutherland GR, Scheffer IE, Berkovic SF, Macdonald RL, Mulley JC.. GABRD encoding a protein for extra- or peri-synaptic GABAA receptors is a susceptibility locus for generalized epilepsies.. Hum Mol Genet, 13(13), 1315-9, 2004.

Feng H-J, Bianchi MT, Macdonald RL. Pentobarbital differentially modulates 13 and 132L GABAA receptor currents. . Mol Pharm, 66, 988-1003, 2004.

Feng H-J, Macdonald RL:. Proton modulation of 13 GABAA receptor channel gating and desensitization.. Neurophysiol , 92, 1577-1585, 2004.

Feng, Hua-Jun, Bianchi, Matt T., Macdonald, Robert L.. PENTOBARBITAL DIFFERENTIALLY MODULATES {alpha}1{beta}3{delta} AND {alpha}1{beta}3{gamma}2L GABAA RECEPTOR CURRENTS. Mol Pharmacol, , , 2004.

Feng, Hua-Jun, Macdonald, Robert L. Multiple actions of propofol on alphabetagamma and alphabetadelta GABAA receptors. Mol Pharmacol, 66(6), 1517-24, 2004.

Feng, Hua-Jun, Macdonald, Robert L.. PROTON MODULATION OF {alpha}1{beta}3{delta} GABAA RECEPTOR CHANNEL GATING AND DESENSITIZATION. J Neurophysiol, , , 2004.

Gallagher MJ, Song L, Macdonald RL. An autosomal dominant juvenile myoclonic epilepsy GABAA receptor 1 subunit mutation produces asymmetrical, subunit position-dependent reduction of heterozygous receptor currents. . J Neurosci, 24, 5570-5578, 2004.

Gallagher, Martin J, Song, Luyan, Arain, Fazal, Macdonald, Robert L. The juvenile myoclonic epilepsy GABA(A) receptor alpha1 subunit mutation A322D produces asymmetrical, subunit position-dependent reduction of heterozygous receptor currents and alpha1 subunit protein expression. J Neurosci, 24(24), 5570-8, 2004.

Kang, Jingqiong, Macdonald, Robert L. The GABAA receptor gamma2 subunit R43Q mutation linked to childhood absence epilepsy and febrile seizures causes retention of alpha1beta2gamma2S receptors in the endoplasmic reticulum. J Neurosci, 24(40), 8672-7, 2004.

Macdonald, Robert L, Gallagher, Martin J, Feng, Hua-Jun, Kang, Jingqiong. GABA(A) receptor epilepsy mutations. Biochem Pharmacol, 68(8), 1497-506, 2004.

Najm I, Ying Z, Babb T, Crino PB, 4 Macdonald R, Mathern GW, Spreafico R.. Mechanisms of epileptogenicity in cortical dysplasias. Neurology, 62(6 Suppl 3)(23), S9-13, 2004.

Bianchi MT, Macdonald RL. Neurosteroids increase the efficacy of GABAA receptor partial agonists. . J Neurosci, 23, 10934-10943, 2003.

Bianchi, Matt T, Macdonald, Robert L. Neurosteroids shift partial agonist activation of GABA(A) receptor channels from low- to high-efficacy gating patterns. J Neurosci, 23(34), 10934-43, 2003.

Hinkle, David J, Bianchi, Matt T, Macdonald, Robert L. Modifications of a commercial perfusion system for use in ultrafast solution exchange during patch clamp recording. Biotechniques, 35(3), 472-4, 476, 2003.

Hinkle, David J, Macdonald, Robert L. Beta subunit phosphorylation selectively increases fast desensitization and prolongs deactivation of alpha1beta1gamma2L and alpha1beta3gamma2L GABA(A) receptor currents. J Neurosci, 23(37), 11698-710, 2003.

Kim, H, Flanagin, BA, Qin, C, Macdonald, RL, Stitzel, JA. The mouse Chrna4 A529T polymorphism alters the ratio of high to low affinity alpha4beta2 nAChRs. Neuropharmacology, (45), 345-354, 2003.

Kim, Heejeong, Macdonald, Robert L. An N-terminal histidine is the primary determinant of alpha subunit-dependent Cu2+ sensitivity of alphabeta3gamma2L GABA(A) receptors. Mol Pharmacol, 64(5), 1145-52, 2003.

Macdonald, Robert L, Bianch, Matt T, Feng, Huajun. Mutations linked to generalized epilepsy in humans reduce GABA(A) receptor current. Exp Neurol, 184 Suppl 1, S58-67, 2003.

Bianchi MT, Macdonald RL. 1 and 6 subunits specify distinct desensitization, deactivation and neurosteroid modulation of GABAA receptors containing the  subunit, . Neuropharmacology, 43, 492-502, 2002.

Bianchi, M T, Haas, K F, Macdonald, R L. Alpha1 and alpha6 subunits specify distinct desensitization, deactivation and neurosteroid modulation of GABA(A) receptors containing the delta subunit. Neuropharmacology, 43(4), 492-502, 2002.

Bianchi, Matt T, Macdonald, Robert L. Slow phases of GABA(A) receptor desensitization: structural determinants and possible relevance for synaptic function. J Physiol, 544(Pt 1), 3-18, 2002. PMCID:2290568

Bianchi, Matt T, Song, Luyan, Zhang, Helen, Macdonald, Robert L. Two different mechanisms of disinhibition produced by GABAA receptor mutations linked to epilepsy in humans. J Neurosci, 22(13), 5321-7, 2002.

Buhr, Andreas, Bianchi, Matt T, Baur, Roland, Courtet, Philippe, Pignay, Virginie, Boulenger, Jean P, Gallati, Sabina, Hinkle, David J, Macdonald, Robert L, Sigel, Erwin. Functional characterization of the new human GABA(A) receptor mutation beta3(R192H). Hum Genet, 111(2), 154-60, 2002.

Chen, Chunling, Bharucha, Vandana, Chen, Yuan, Westenbroek, Ruth E, Brown, Angus, Malhotra, Jyoti Dhar, Jones, Dorothy, Avery, Christy, Gillespie, Patrick J, Kazen-Gillespie, Kristin A, Kazarinova-Noyes, Katie, Shrager, Peter, Saunders, Thomas L, Macdonald, Robert L, Ransom, Bruce R, Scheuer, Todd, Catterall, William A, Isom, Lori L. Reduced sodium channel density, altered voltage dependence of inactivation, and increased susceptibility to seizures in mice lacking sodium channel beta 2-subunits. Proc Natl Acad Sci U S A, 99(26), 17072-7, 2002. PMCID:139271

Greenfield, L John, Zaman, Shahid H, Sutherland, Margaret L, Lummis, Sarah C R, Niemeyer, Mar?-a-Isabel, Barnard, Eric A, Macdonald, Robert L. Mutation of the GABAA receptor M1 transmembrane proline increases GABA affinity and reduces barbiturate enhancement. Neuropharmacology, 42(4), 502-21, 2002.

Jones, Dorothy M, Esmaeil, Nadia, Maren, Stephen, Macdonald, Robert L. Characterization of pharmacoresistance to benzodiazepines in the rat Li-pilocarpine model of status epilepticus. Epilepsy Res, 50(3), 301-12, 2002.

Wohlfarth, Kai M, Bianchi, Matt T, Macdonald, Robert L. Enhanced neurosteroid potentiation of ternary GABA(A) receptors containing the delta subunit. J Neurosci, 22(5), 1541-9, 2002.

Bianchi MT, Haas K, Macdonald RL. Structural determinants of fast desensitization and desensitization-deactivation coupling in GABAA receptors. . J Neurosci , 21, 1127-1136, 2001.

Bianchi MT, Macdonald RL. Mutation of the 9?Leucine in the GABAA receptor 2L subunit produces an apparent decrease in desensitization by stabilizing open states without altering desensitized states. . Neuropharmacology, 41, 737-734, 2001.

Bianchi, M T, Haas, K F, Macdonald, R L. Structural determinants of fast desensitization and desensitization-deactivation coupling in GABAa receptors. J Neurosci, 21(4), 1127-36, 2001.

Bianchi, M T, Macdonald, R L. Agonist Trapping by GABAA Receptor Channels. J Neurosci, 21(23), 9083-91, 2001.

Bianchi, M T, Macdonald, R L. Mutation of the 9' leucine in the GABA(A) receptor gamma2L subunit produces an apparent decrease in desensitization by stabilizing open states without altering desensitized states. Neuropharmacology, 41(6), 737-44, 2001.

Kelly, K M, Kume, A, Albin, R L, Macdonald, R L. Autoradiography of L-type and N-type calcium channels in aged rat hippocampus, entorhinal cortex, and neocortex. Neurobiol Aging, 22(1), 17-23, 2001.

Nagaya, N, Macdonald, R L. Two gamma2L subunit domains confer low Zn2+ sensitivity to ternary GABA(A) receptors. J Physiol, 532(Pt 1), 17-30, 2001. PMCID:2278531

. Bianchi MT, Macdonald RL: Agonist trapping by GABAA receptor channels. . J Neurosci, 21, 9083-9091, 2001.

Fisher, J L, Hinkle, D J, Macdonald, R L. Loreclezole inhibition of recombinant alpha1beta1gamma2L GABA(A) receptor single channel currents. Neuropharmacology, 39(2), 235-45, 2000.

Neelands, T R, King, A P, Macdonald, R L. Functional expression of L-, N-, P/Q-, and R-type calcium channels in the human NT2-N cell line. J Neurophysiol, 84(6), 2933-44, 2000.

Burgard, E C, Haas, K F, Macdonald, R L. Channel properties determine the transient activation kinetics of recombinant GABA(A) receptors. Brain Res Mol Brain Res, 73(1-2), 28-36, 1999.

Haas, K F, Macdonald, R L. GABAA receptor subunit gamma2 and delta subtypes confer unique kinetic properties on recombinant GABAA receptor currents in mouse fibroblasts. J Physiol, 514 ( Pt 1), 27-45, 1999. PMCID:2269054

Kapur, J, Haas, K F, Macdonald, R L. Physiological properties of GABAA receptors from acutely dissociated rat dentate granule cells. J Neurophysiol, 81(5), 2464-71, 1999.

Kapur, J, Macdonald, R L. Postnatal development of hippocampal dentate granule cell gamma-aminobutyric acidA receptor pharmacological properties. Mol Pharmacol, 55(3), 444-52, 1999.

King, A P, Hall, K E, Macdonald, R L. kappa- and mu-Opioid inhibition of N-type calcium currents is attenuated by 4beta-phorbol 12-myristate 13-acetate and protein kinase C in rat dorsal root ganglion neurons. J Pharmacol Exp Ther, 289(1), 312-20, 1999.

Lowenstein, D H, Bleck, T, Macdonald, R L. It's time to revise the definition of status epilepticus. Epilepsia, 40(1), 120-2, 1999.

MacDonald, R L, Kapur, J. Pharmacological properties of recombinant and hippocampal dentate granule cell GABAA receptors. Adv Neurol, 79, 979-90, 1999.

Macdonald, R L, Kapur, J. Acute cellular alterations in the hippocampus after status epilepticus. Epilepsia, 40 Suppl 1, S9-20; discussion S21-2, 1999.

Neelands TR, Macdonald R. Incorporation of the pi subunit into functional GABAA receptor channels. Mol Pharmacol, 56, 598-610, 1999.

Neelands TR, Zhang J, Macdonald RL. GABAA receptors expressed in undifferentiated human teratocarcinoma NT2 stem cells differ from those expressed by differentiated NT2- N cells. J Neurosci, 19, 7057-7065, 1999.

Neelands, T R, Fisher, J L, Bianchi, M, Macdonald, R L. Spontaneous and gamma-aminobutyric acid (GABA)-activated GABA(A) receptor channels formed by epsilon subunit-containing isoforms. Mol Pharmacol, 55(1), 168-78, 1999.

Tietz EI, Kapur J, Macdonald RL. Functional GABAA receptor heterogeneity of acutely dissociated hippocampal CA1 pyramidal cell. J Neurophysiol , 81, 1575-1586, 1999.

Fisher JL, Macdonald RL. The role of an alpha subtype M2-M3 His in regulating inhibition of GABAA receptor current by zinc and other divalent cations. J Neurosci, 18, 2944-2953, 1998.

Neelands, T R, Greenfield, L J, Zhang, J, Turner, R S, Macdonald, R L. GABAA receptor pharmacology and subtype mRNA expression in human neuronal NT2-N cells. J Neurosci, 18(13), 4993-5007, 1998.

Fisher JL, Macdonald RL. Single channel properties of recombinant GABAA receptors containing gamma2 or delta subtypes expressed with alpha1 and beta3 subtypes in L929 cells. J Physiol (Lond), 505, 283-297, 1997.

Fisher JL, Macdonald Rl. Functional properties of recombinant GABAA receptors composed of single or multiple beta subunit subtypes. Neuropharmacology , 36, 1601-1610, 1997.

Fisher JL, Zhang J, Macdonald RL. The role of alpha1 and alpha6 subtype amino-terminal domains in allosteric regulation of gamma-aminobutyric acidA receptors. Mol Pharmacol , 52, 714-724, 1997.

Greenfield, L J, Sun, F, Neelands, T R, Burgard, E C, Donnelly, J L, MacDonald, R L. Expression of functional GABAA receptors in transfected L929 cells isolated by immunomagnetic bead separation. Neuropharmacology, 36(1), 63-73, 1997.

Kapur J, Macdonald RL. Rapid seizure-induced reduction of benzodiazepine and Zn2+ sensitivity of hippocampal dentate granule cell GABAA receptors. J Neurosci, 17, 7532-7540, 1997.

Macdonald, R L, Greenfield, L J. Mechanisms of action of new antiepileptic drugs. Curr Opin Neurol, 10(2), 121-8, 1997.

Saxena NC, Neelands TR, Macdonald RL. Contrasting actions of lanthanum on different recombinant gamma-aminobutyric acidA receptor isoforms expressed in L929 fibroblasts. Mol Pharmacol, 51, 328-335, 1997.

Wiley, J W, Moises, H C, Gross, R A, MacDonald, R L. Dynorphin A-mediated reduction in multiple calcium currents involves a G(o) alpha-subtype G protein in rat primary afferent neurons. J Neurophysiol, 77(3), 1338-48, 1997.

Burgard, E C, Tietz, E I, Neelands, T R, Macdonald, R L. Properties of recombinant gamma-aminobutyric acid A receptor isoforms containing the alpha 5 subunit subtype. Mol Pharmacol, 50(1), 119-27, 1996.

Donnelly, J L, MacDonald, R L. Loreclezole enhances apparent desensitization of recombinant GABAA receptor currents. Neuropharmacology, 35(9-10), 1233-41, 1996.

Kapur, J, Macdonald, R L. Cyclic AMP-dependent protein kinase enhances hippocampal dentate granule cell GABAA receptor currents. J Neurophysiol, 76(4), 2626-34, 1996.

Kapur, J, Macdonald, R L. Pharmacological properties of gamma-aminobutyric acidA receptors from acutely dissociated rat dentate granule cells. Mol Pharmacol, 50(3), 458-66, 1996.

Kume, A, Greenfield, L J, Macdonald, R L, Albin, R L. Felbamate inhibits [3H]t-butylbicycloorthobenzoate (TBOB) binding and enhances Cl- current at the gamma-aminobutyric AcidA (GABAA) receptor. J Pharmacol Exp Ther, 277(3), 1784-92, 1996.

Lin, Y F, Angelotti, T P, Dudek, E M, Browning, M D, Macdonald, R L. Enhancement of recombinant alpha 1 beta 1 gamma 2L gamma-aminobutyric acidA receptor whole-cell currents by protein kinase C is mediated through phosphorylation of both beta 1 and gamma 2L subunits. Mol Pharmacol, 50(1), 185-95, 1996.

Macdonald, R L. Is there a mechanistic basis for rational polypharmacy?. Epilepsy Res Suppl, 11, 79-93, 1996.

Macdonald, R L, Saxena, N C, Angelotti, T P. Functional expression of recombinant GABAA receptor channels in L929 fibroblasts. Epilepsy Res Suppl, 12, 177-85, 1996.

Saxena, N C, Macdonald, R L. Properties of putative cerebellar gamma-aminobutyric acid A receptor isoforms. Mol Pharmacol, 49(3), 567-79, 1996.

Hall, K E, Browning, M D, Dudek, E M, Macdonald, R L. Enhancement of high threshold calcium currents in rat primary afferent neurons by constitutively active protein kinase C. J Neurosci, 15(9), 6069-76, 1995.

Macdonald, R L. Ethanol, gamma-aminobutyrate type A receptors, and protein kinase C phosphorylation. Proc Natl Acad Sci U S A, 92(9), 3633-5, 1995. PMCID:42016

Macdonald, R L, Kelly, K M. Antiepileptic drug mechanisms of action. Epilepsia, 36 Suppl 2, S2-12, 1995.

Massa, E, Kelly, K M, Yule, D I, MacDonald, R L, Uhler, M D. Comparison of fura-2 imaging and electrophysiological analysis of murine calcium channel alpha 1 subunits coexpressed with novel beta 2 subunit isoforms. Mol Pharmacol, 47(4), 707-16, 1995.

Pennell, P B, Ogaily, M S, Macdonald, R L. Aplastic anemia in a patient receiving felbamate for complex partial seizures. Neurology, 45(3 Pt 1), 456-60, 1995.

Rock, D M, Macdonald, R L. Polyamine regulation of N-methyl-D-aspartate receptor channels. Annu Rev Pharmacol Toxicol, 35, 463-82, 1995.

Lin, Y F, Browning, M D, Dudek, E M, Macdonald, R L. Protein kinase C enhances recombinant bovine alpha 1 beta 1 gamma 2L GABAA receptor whole-cell currents expressed in L929 fibroblasts. Neuron, 13(6), 1421-31, 1994.

Macdonald, R L, Kelly, K M. Mechanisms of action of currently prescribed and newly developed antiepileptic drugs. Epilepsia, 35 Suppl 4, S41-50, 1994.

Macdonald, R L, Olsen, R W. GABAA receptor channels. Annu Rev Neurosci, 17, 569-602, 1994.

Moises, H C, Rusin, K I, Macdonald, R L. mu-Opioid receptor-mediated reduction of neuronal calcium current occurs via a G(o)-type GTP-binding protein. J Neurosci, 14(6), 3842-51, 1994.

Moises, H C, Rusin, K I, Macdonald, R L. Mu- and kappa-opioid receptors selectively reduce the same transient components of high-threshold calcium current in rat dorsal root ganglion sensory neurons. J Neurosci, 14(10), 5903-16, 1994.

Rogers, C J, Twyman, R E, Macdonald, R L. Benzodiazepine and beta-carboline regulation of single GABAA receptor channels of mouse spinal neurones in culture. J Physiol, 475(1), 69-82, 1994. PMCID:1160356

Saxena, N C, Macdonald, R L. Assembly of GABAA receptor subunits: role of the delta subunit. J Neurosci, 14(11 Pt 2), 7077-86, 1994.

Angelotti, T P, Macdonald, R L. Assembly of GABAA receptor subunits: alpha 1 beta 1 and alpha 1 beta 1 gamma 2S subunits produce unique ion channels with dissimilar single-channel properties. J Neurosci, 13(4), 1429-40, 1993.

Angelotti, T P, Uhler, M D, Macdonald, R L. Assembly of GABAA receptor subunits: analysis of transient single-cell expression utilizing a fluorescent substrate/marker gene technique. J Neurosci, 13(4), 1418-28, 1993.

Angelotti, T P, Uhler, M D, Macdonald, R L. Enhancement of recombinant gamma-aminobutyric acid type A receptor currents by chronic activation of cAMP-dependent protein kinase. Mol Pharmacol, 44(6), 1202-10, 1993.

Macdonald, R L, Kelly, K M. Antiepileptic drug mechanisms of action. Epilepsia, 34 Suppl 5, S1-8, 1993.

Rock, D M, Kelly, K M, Macdonald, R L. Gabapentin actions on ligand- and voltage-gated responses in cultured rodent neurons. Epilepsy Res, 16(2), 89-98, 1993.

Wiley, J W, Gross, R A, MacDonald, R L. Agonists for neuropeptide Y receptor subtypes NPY-1 and NPY-2 have opposite actions on rat nodose neuron calcium currents. J Neurophysiol, 70(1), 324-30, 1993.

Angelotti, T P, Tan, F, Chahine, K G, Macdonald, R L. Molecular and electrophysiological characterization of a allelic variant of the rat alpha 6 GABAA receptor subunit. Brain Res Mol Brain Res, 16(1-2), 173-8, 1992.

MacDonald, R L, Twyman, R E. Kinetic properties and regulation of GABAA receptor channels. Ion Channels, 3, 315-43, 1992.

Macdonald, R L, Twyman, R E, Ryan-Jastrow, T, Angelotti, T P. Regulation of GABAA receptor channels by anticonvulsant and convulsant drugs and by phosphorylation. Epilepsy Res Suppl, 9, 265-77, 1992.

Porter, N M, Angelotti, T P, Twyman, R E, MacDonald, R L. Kinetic properties of alpha 1 beta 1 gamma-aminobutyric acidA receptor channels expressed in Chinese hamster ovary cells: regulation by pentobarbital and picrotoxin. Mol Pharmacol, 42(5), 872-81, 1992.

Rock, D M, MacDonald, R L. Spermine and related polyamines produce a voltage-dependent reduction of N-methyl-D-aspartate receptor single-channel conductance. Mol Pharmacol, 42(1), 157-64, 1992.

Rock, D M, Macdonald, R L. The polyamine spermine has multiple actions on N-methyl-D-aspartate receptor single-channel currents in cultured cortical neurons. Mol Pharmacol, 41(1), 83-8, 1992.

Rock, D M, Macdonald, R L. The polyamine diaminodecane (DA-10) produces a voltage-dependent flickery block of single NMDA receptor channels. Neurosci Lett, 144(1-2), 111-5, 1992.

Twyman, R E, Green, R M, MacDonald, R L. Kinetics of open channel block by penicillin of single GABAA receptor channels from mouse spinal cord neurones in culture. J Physiol, 445, 97-127, 1992. PMCID:1179972

Twyman, R E, Macdonald, R L. Neurosteroid regulation of GABAA receptor single-channel kinetic properties of mouse spinal cord neurons in culture. J Physiol, 456, 215-45, 1992. PMCID:1175679

Wiley, J W, Gross, R A, MacDonald, R L. The peptide CGRP increases a high-threshold Ca2+ current in rat nodose neurones via a pertussis toxin-sensitive pathway. J Physiol, 455, 367-81, 1992. PMCID:1175649

De Deyn, P P, Marescau, B, Macdonald, R L. Guanidino compounds that are increased in hyperargininemia inhibit GABA and glycine responses on mouse neurons in cell culture. Epilepsy Res, 8(2), 134-41, 1991.

Kelly, K M, Gross, R A, Macdonald, R L. Tetrahydroaminoacridine (THA) reduces voltage-dependent calcium currents in rat sensory neurons. Neurosci Lett, 132(2), 247-50, 1991.

Rock, D M, McLean, M J, Macdonald, R L, Catterall, W A, Taylor, C P. Ralitoline (CI-946) and CI-953 block sustained repetitive sodium action potentials in cultured mouse spinal cord neurons and displace batrachotoxinin A 20-alpha-benzoate binding in vitro. Epilepsy Res, 8(3), 197-203, 1991.

Twyman, R E, Macdonald, R L. Kinetic properties of the glycine receptor main- and sub-conductance states of mouse spinal cord neurones in culture. J Physiol, 435, 303-31, 1991. PMCID:1181464

De Deyn, P P, Macdonald, R L. Guanidino compounds that are increased in cerebrospinal fluid and brain of uremic patients inhibit GABA and glycine responses on mouse neurons in cell culture. Ann Neurol, 28(5), 627-33, 1990.

De Deyn, P P, Marescau, B, MacDonald, R L. Epilepsy and the GABA-hypothesis a brief review and some examples. Acta Neurol Belg, 90(2), 65-81, 1990.

Gross, R A, Moises, H C, Uhler, M D, Macdonald, R L. Dynorphin A and cAMP-dependent protein kinase independently regulate neuronal calcium currents. Proc Natl Acad Sci U S A, 87(18), 7025-9, 1990. PMCID:54675

Gross, R A, Uhler, M D, Macdonald, R L. The reduction of neuronal calcium currents by ATP-gamma-S is mediated by a G protein and occurs independently of cyclic AMP-dependent protein kinase. Brain Res, 535(2), 214-20, 1990.

Gross, R A, Uhler, M D, Macdonald, R L. The cyclic AMP-dependent protein kinase catalytic subunit selectively enhances calcium currents in rat nodose neurones. J Physiol, 429, 483-96, 1990. PMCID:1181711

Gross, R A, Wiley, J W, Ryan-Jastrow, T, Macdonald, R L. Regulation by GTP and its stable thiol derivatives of calcium current components in rat nodose ganglion neurons. Mol Pharmacol, 37(4), 546-53, 1990.

Kelly, K M, Gross, R A, Macdonald, R L. Valproic acid selectively reduces the low-threshold (T) calcium current in rat nodose neurons. Neurosci Lett, 116(1-2), 233-8, 1990.

Moss, S J, Smart, T G, Porter, N M, Nayeem, N, Devine, J, Stephenson, F A, Macdonald, R L, Barnard, E A. Cloned GABA receptors are maintained in a stable cell line: allosteric and channel properties. Eur J Pharmacol, 189(1), 77-88, 1990.

Porter, N M, Twyman, R E, Uhler, M D, Macdonald, R L. Cyclic AMP-dependent protein kinase decreases GABAA receptor current in mouse spinal neurons. Neuron, 5(6), 789-96, 1990.

Twyman RE, Rogers CJ, Macdonald RL. Intraburst kinetic properties of the GABAA receptor main conductance state of mouse spinal cord neurones in culture. . J Physiology (Lond) , 423, 193-219, 1990.

Twyman, R E, Rogers, C J, Macdonald, R L. Intraburst kinetic properties of the GABAA receptor main conductance state of mouse spinal cord neurones in culture. J Physiol, 423, 193-220, 1990. PMCID:1189753

Wiley, J W, Gross, R A, Lu, Y X, Macdonald, R L. Neuropeptide Y reduces calcium current and inhibits acetylcholine release in nodose neurons via a pertussis toxin-sensitive mechanism. J Neurophysiol, 63(6), 1499-507, 1990.

De Deyn, P P, Macdonald, R L. Effects of antiepileptic drugs on GABA responses and on reduction of GABA responses by PTZ and DMCM on mouse neurons in cell culture. Epilepsia, 30(1), 17-25, 1989.

Gross, R A, MacDonald, R L. Activators of protein kinase C selectively enhance inactivation of a calcium current component of cultured sensory neurons in a pertussis toxin-sensitive manner. J Neurophysiol, 61(6), 1259-69, 1989.

Gross, R A, Macdonald, R L. Cyclic AMP selectively reduces the N-type calcium current component of mouse sensory neurons in culture by enhancing inactivation. J Neurophysiol, 61(1), 97-105, 1989.

Gross, R A, Macdonald, R L, Ryan-Jastrow, T. 2-Chloroadenosine reduces the N calcium current of cultured mouse sensory neurones in a pertussis toxin-sensitive manner. J Physiol, 411, 585-95, 1989. PMCID:1190543

MacDonald, R L, Rogers, C J, Twyman, R E. Barbiturate regulation of kinetic properties of the GABAA receptor channel of mouse spinal neurones in culture. J Physiol, 417, 483-500, 1989. PMCID:1189279

Macdonald RL, Rogers CJ, Twyman RE. Kinetic properties of the GABAA receptor main conductance state of mouse spinal cord neurones in cell culture. . J Physiology (Lond.), 410, 479-499, 1989.

Macdonald RL, Rogers CJ, Twyman RE. Barbiturate regulation of kinetic properties of the main conductance state of GABAA receptor channel of mouse spinal neurones in culture.. J Physiology (Lond., 417, 483-500, 1989.

Macdonald, R L. Antiepileptic drug actions. Epilepsia, 30 Suppl 1, S19-28; discussion S64-8, 1989.

Macdonald, R L, Rogers, C J, Twyman, R E. Kinetic properties of the GABAA receptor main conductance state of mouse spinal cord neurones in culture. J Physiol, 410, 479-99, 1989. PMCID:1190491

Rock, D M, Macdonald, R L, Taylor, C P. Blockade of sustained repetitive action potentials in cultured spinal cord neurons by zonisamide (AD 810, CI 912), a novel anticonvulsant. Epilepsy Res, 3(2), 138-43, 1989.

Twyman, R E, Rogers, C J, Macdonald, R L. Differential regulation of gamma-aminobutyric acid receptor channels by diazepam and phenobarbital. Ann Neurol, 25(3), 213-20, 1989.

Twyman, R E, Rogers, C J, Macdonald, R L. Pentobarbital and picrotoxin have reciprocal actions on single GABAA receptor channels. Neurosci Lett, 96(1), 89-95, 1989.

De Deyn, P P, Macdonald, R L. Effects of non-sedative anxiolytic drugs on responses to GABA and on diazepam-induced enhancement of these responses on mouse neurones in cell culture. Br J Pharmacol, 95(1), 109-20, 1988. PMCID:1854132

De Deyn, P P, Marescau, B, Macdonald, R L. Effects of alpha-keto-delta-guanidinovaleric acid on inhibitory amino acid responses on mouse neurons in cell culture. Brain Res, 449(1-2), 54-60, 1988.

Gross, R A, Macdonald, R L. Barbiturates and nifedipine have different and selective effects on calcium currents of mouse DRG neurons in culture: a possible basis for differing clinical actions. The 1987 S. Weir Mitchell award. Neurology, 38(3), 443-51, 1988.

Gross, R A, Macdonald, R L. Differential actions of pentobarbitone on calcium current components of mouse sensory neurones in culture. J Physiol, 405, 187-203, 1988. PMCID:1190971

Gross, R A, Macdonald, R L. Reduction of the same calcium current component by A and C kinases: differential pertussis toxin sensitivity. Neurosci Lett, 88(1), 50-6, 1988.

Macdonald, R L. Anticonvulsant drug actions on neurons in cell culture. J Neural Transm, 72(3), 173-83, 1988.

Macdonald, R L, Twyman, R E, Rogers, C J, Weddle, M G. Pentobarbital regulation of the kinetic properties of GABA receptor chloride channels. Adv Biochem Psychopharmacol, 45, 61-71, 1988.

McLean, M J, Macdonald, R L. Benzodiazepines, but not beta carbolines, limit high frequency repetitive firing of action potentials of spinal cord neurons in cell culture. J Pharmacol Exp Ther, 244(2), 789-95, 1988.

De Deyn, P P, Macdonald, R L. CGS 9896 and ZK 91296, but not CGS 8216 and RO 15-1788, are pure benzodiazepine receptor antagonists on mouse neurons in culture. J Pharmacol Exp Ther, 242(1), 48-55, 1987.

Grega, D S, Macdonald, R L. Activators of adenylate cyclase and cyclic AMP prolong calcium-dependent action potentials of mouse sensory neurons in culture by reducing a voltage-dependent potassium conductance. J Neurosci, 7(3), 700-7, 1987.

Grega, D S, Werz, M A, Macdonald, R L. Forskolin and phorbol esters reduce the same potassium conductance of mouse neurons in culture. Science, 235(4786), 345-8, 1987.

Gross, R A, Macdonald, R L. Dynorphin A selectively reduces a large transient (N-type) calcium current of mouse dorsal root ganglion neurons in cell culture. Proc Natl Acad Sci U S A, 84(15), 5469-73, 1987. PMCID:298879

Macdonald, R L, McLean, M J. Mechanisms of anticonvulsant drug action. Electroencephalogr Clin Neurophysiol Suppl, 39, 200-8, 1987.

Werz, M A, Grega, D S, MacDonald, R L. Actions of mu, delta and kappa opioid agonists and antagonists on mouse primary afferent neurons in culture. J Pharmacol Exp Ther, 243(1), 258-63, 1987.

Werz, M A, Macdonald, R L. Dual actions of phorbol esters to decrease calcium and potassium conductances of mouse neurons. Neurosci Lett, 78(1), 101-6, 1987.

Werz, M A, Macdonald, R L. Phorbol esters: voltage-dependent effects on calcium-dependent action potentials of mouse central and peripheral neurons in cell culture. J Neurosci, 7(6), 1639-47, 1987.

MacDonald, R L, Skerritt, J H, Werz, M A. Adenosine agonists reduce voltage-dependent calcium conductance of mouse sensory neurones in cell culture. J Physiol, 370, 75-90, 1986. PMCID:1192669

Macdonald, R L, McLean, M J. Anticonvulsant drugs: mechanisms of action. Adv Neurol, 44, 713-36, 1986.

Macdonald, R L, Weddle, M G, Gross, R A. Benzodiazepine, beta-carboline, and barbiturate actions on GABA responses. Adv Biochem Psychopharmacol, 41, 67-78, 1986.

Macdonald, R L, Werz, M A. Dynorphin A decreases voltage-dependent calcium conductance of mouse dorsal root ganglion neurones. J Physiol, 377, 237-49, 1986. PMCID:1182830

McLean, M J, Macdonald, R L. Sodium valproate, but not ethosuximide, produces use- and voltage-dependent limitation of high frequency repetitive firing of action potentials of mouse central neurons in cell culture. J Pharmacol Exp Ther, 237(3), 1001-11, 1986.

McLean, M J, Macdonald, R L. Carbamazepine and 10,11-epoxycarbamazepine produce use- and voltage-dependent limitation of rapidly firing action potentials of mouse central neurons in cell culture. J Pharmacol Exp Ther, 238(2), 727-38, 1986.

Macdonald, R L, McLean, M J, Skerritt, J H. Anticonvulsant drug mechanisms of action. Fed Proc, 44(10), 2634-9, 1985.

McLean, M J, MacDonald, R L. Cellular mechanistic bases for classification of clinically useful anticonvulsant drugs. Ann Acad Med Singapore, 14(1), 128-36, 1985.

Skerritt, J H, Johnston, G A, Chow, S C, MacDonald, R L, Prager, R H, Ward, A D. Differential modulation of gamma-aminobutyric acid receptors by caprolactam derivatives with central nervous system depressant or convulsant activity. Brain Res, 331(2), 225-33, 1985.

Werz, M A, Macdonald, R L. Barbiturates decrease voltage-dependent calcium conductance of mouse neurons in dissociated cell culture. Mol Pharmacol, 28(3), 269-77, 1985.

Werz, M A, Macdonald, R L. Dynorphin and neoendorphin peptides decrease dorsal root ganglion neuron calcium-dependent action potential duration. J Pharmacol Exp Ther, 234(1), 49-56, 1985.

Skerritt, J H, Macdonald, R L. Diazepam enhances the action but not the binding of the GABA analog, THIP. Brain Res, 297(1), 181-6, 1984.

Skerritt, J H, Macdonald, R L. Benzodiazepine receptor ligand actions on GABA responses. Beta-carbolines, purines. Eur J Pharmacol, 101(1-2), 135-41, 1984.

Skerritt, J H, Macdonald, R L. Benzodiazepine receptor ligand actions on GABA responses. Benzodiazepines, CL 218872, zopiclone. Eur J Pharmacol, 101(1-2), 127-34, 1984.

Skerritt, J H, Macdonald, R L. Multiple actions of convulsant barbiturates on mouse neurons in cell culture. J Pharmacol Exp Ther, 230(1), 82-8, 1984.

Skerritt, J H, Werz, M A, McLean, M J, Macdonald, R L. Diazepam and its anomalous p-chloro-derivative Ro 5-4864: comparative effects on mouse neurons in cell culture. Brain Res, 310(1), 99-105, 1984.

Werz, M A, Macdonald, R L. Dynorphin reduces voltage-dependent calcium conductance of mouse dorsal root ganglion neurons. Neuropeptides, 5(1-3), 253-6, 1984.

Werz, M A, Macdonald, R L. Dynorphin reduces calcium-dependent action potential duration by decreasing voltage-dependent calcium conductance. Neurosci Lett, 46(2), 185-90, 1984.

Bergey, G K, MacDonald, R L, Habig, W H, Hardegree, M C, Nelson, P G. Tetanus toxin: convulsant action on mouse spinal cord neurons in culture. J Neurosci, 3(11), 2310-23, 1983.

Macdonald, R L, Pun, R Y, Neale, E A, Nelson, P G. Synaptic interactions between mammalian central neurons in cell culture. I. Reversal potential for excitatory postsynaptic potentials. J Neurophysiol, 49(6), 1428-41, 1983.

McLean, M J, Macdonald, R L. Multiple actions of phenytoin on mouse spinal cord neurons in cell culture. J Pharmacol Exp Ther, 227(3), 779-89, 1983.

Neale, E A, Nelson, P G, Macdonald, R L, Christian, C N, Bowers, L M. Synaptic interactions between mammalian central neurons in cell culture. III. Morphophysiological correlates of quantal synaptic transmission. J Neurophysiol, 49(6), 1459-68, 1983.

Nelson, P G, Marshall, K C, Pun, R Y, Christian, C N, Sheriff, W H, Macdonald, R L, Neale, E A. Synaptic interactions between mammalian central neurons in cell culture. II. Quantal Analysis of EPSPs. J Neurophysiol, 49(6), 1442-58, 1983.

Nowak, L M, Macdonald, R L. Muscarine-sensitive voltage-dependent potassium current in cultured murine spinal cord neurons. Neurosci Lett, 35(1), 85-91, 1983.

Nowak, L M, Macdonald, R L. Ionic mechanism of muscarinic cholinergic depolarization of mouse spinal cord neurons in cell culture. J Neurophysiol, 49(3), 792-803, 1983.

Skerritt, J H, Macdonald, R L. Benzodiazepine Ro 15-1788: electrophysiological evidence for partial agonist activity. Neurosci Lett, 43(2-3), 321-6, 1983.

Werz, M A, MacDonald, R L. Opioid peptides selective for mu- and delta-opiate receptors reduce calcium-dependent action potential duration by increasing potassium conductance. Neurosci Lett, 42(2), 173-8, 1983.

Werz, M A, Macdonald, R L. Opioid peptides with differential affinity for mu and delta receptors decrease sensory neuron calcium-dependent action potentials. J Pharmacol Exp Ther, 227(2), 394-402, 1983.

Frere, R C, Macdonald, R L, Young, A B. GABA binding and bicuculline in spinal cord and cortical membranes from adult rat and from mouse neurons in cell culture. Brain Res, 244(1), 145-53, 1982.

Heyer, E J, Macdonald, R L. Barbiturate reduction of calcium-dependent action potentials: correlation with anesthetic action. Brain Res, 236(1), 157-71, 1982.

Heyer, E J, Macdonald, R L. Calcium- and sodium-dependent action potentials of mouse spinal cord and dorsal root ganglion neurons in cell culture. J Neurophysiol, 47(4), 641-55, 1982.

Heyer, E J, Nowak, L M, Macdonald, R L. Membrane depolarization and prolongation of calcium-dependent action potentials of mouse neurons in cell culture by two convulsants: bicuculline and penicillin. Brain Res, 232(1), 41-56, 1982.

Macdonald, R L, McLean, M J. Cellular bases of barbiturate and phenytoin anticonvulsant drug action. Epilepsia, 23 Suppl 1, S7-18, 1982.

Macdonald, R L, Moonen, G, Neale, E A, Nelson, P G. Cerebellar macroneurons in microexplant cell culture. Postsynaptic amino acid pharmacology. Brain Res, 281(1), 75-88, 1982.

Moonen, G, Neale, E A, Macdonald, R L, Gibbs, W, Nelson, P G. Cerebellar macroneurons in microexplant cell culture. Methodology, basic electrophysiology, and morphology after horseradish peroxidase injection. Brain Res, 281(1), 59-73, 1982.

Neale, E A, Moonen, G, Macdonald, R L, Nelson, P G. Cerebellar macroneurons in microexplant cell culture: ultrastructural morphology. Neuroscience, 7(8), 1879-90, 1982.

Nowak, L M, Macdonald, R L. Substance P: ionic basis for depolarizing responses of mouse spinal cord neurons in cell culture. J Neurosci, 2(8), 1119-28, 1982.

Nowak, L M, Young, A B, Macdonald, R L. GABA and bicuculline actions on mouse spinal cord and cortical neurons in cell culture. Brain Res, 244(1), 155-64, 1982.

Werz, M A, Macdonald, R L. Opiate alkaloids antagonize postsynaptic glycine and GABA responses: correlation with convulsant action. Brain Res, 236(1), 107-19, 1982.

Werz, M A, Macdonald, R L. Opioid peptides decrease calcium-dependent action potential duration of mouse dorsal root ganglion neurons in cell culture. Brain Res, 239(1), 315-21, 1982.

Werz, M A, Macdonald, R L. Heterogeneous sensitivity of cultured dorsal root ganglion neurones to opioid peptides selective for mu- and delta-opiate receptors. Nature, 299(5885), 730-3, 1982.

Heyer, E J, MacDonald, R L, Bergey, G K, Nelson, P G. Calcium-dependent action potentials in mouse spinal cord neurons in cell culture. Brain Res, 220(2), 408-15, 1981.

Heyer, E J, Nowak, L M, Macdonald, R L. Bicuculline: a convulsant with synaptic and nonsynaptic actions. Neurology, 31(11), 1381-90, 1981.

Macdonald, R L, Nowak, L M. Substance P and somatostatin actions on spinal cord neurons in primary dissociated cell culture. Adv Biochem Psychopharmacol, 28, 159-73, 1981.

Macdonald, R L, Young, A B. Pharmacology of GABA-mediated inhibition of spinal cord neurons in vivo and in primary dissociated cell culture. Mol Cell Biochem, 38 Spec No(Pt 1), 147-62, 1981.

Nowak, L M, MacDonald, R L. Substance P decreases a potassium conductance of spinal cord neurons in cell culture. Brain Res, 214(2), 416-23, 1981.

Schulz, D W, Macdonald, R L. Barbiturate enhancement of GABA-mediated inhibition and activation of chloride ion conductance: correlation with anticonvulsant and anesthetic actions. Brain Res, 209(1), 177-88, 1981.

Macdonald, R L, Nowak, L M. Somatostatin actions in primary dissociated cell cultures of mammalian spinal cord neurons. Int J Neurol, 14(2-4), 221-32, 1980.

Neale, E A, MacDonald, R L, Nelson, P G. An inhibitory spinal cord neuron. JAMA, 244(22), 2555, 1980.

Zimmerman, E A, MacDonald, R L, Vale, W, Faden, A I. Symposium on neuropeptides. Trans Am Neurol Assoc, 105, 504-27, 1980.

MacDonald, R L, Barker, J L. Enhancement of GABA-mediated postsynaptic inhibition in cultured mammalian spinal cord neurons: a common mode of anticonvulsant action. Brain Res, 167(2), 323-36, 1979.

Macdonald, R L, Barker, J L. Anticonvulsant and anesthetic barbiturates: different postsynaptic actions in cultured mammalian neurons. Neurology, 29(4), 432-47, 1979.

Macdonald, R L, Bergey, G K. Valproic acid augments GABA-mediated postsynaptic inhibition in cultured mammalian neurons. Brain Res, 170(3), 558-62, 1979.

Barker, J L, Neale, J H, Smith, T G, Macdonald, R L. Opiate peptide modulation of amino acid responses suggests novel form of neuronal communication. Science, 199(4336), 1451-3, 1978.

Bergey, G K, Macdonald, R L. The porphyrin precursor delta aminolevulinic acid diminishes amino acid responses in cultured murine spinal cord neurons. Trans Am Neurol Assoc, 103, 87-91, 1978.

Macdonald, R L, Barker, J L. Different actions of anticonvulsant and anesthetic barbiturates revealed by use of cultured mammalian neurons. Science, 200(4343), 775-7, 1978.

Macdonald, R L, Barker, J L. Specific antagonism of GABA-mediated postsynaptic inhibition in cultured mammalian spinal cord neurons: a common mode of convulsant action. Neurology, 28(4), 325-30, 1978.

Macdonald, R L, Bergey, G K. Valproic acid: effect on GABA-mediated postsynaptic inhibition in cultured mammalian spinal cord neurons. Trans Am Neurol Assoc, 103, 254-6, 1978.

Macdonald, R L, Nelson, P G. Specific-opiate-induced depression of transmitter release from dorsal root ganglion cells in culture. Science, 199(4336), 1449-51, 1978.

Neale, E A, Macdonald, R L, Nelson, P G. Intracellular horseradish peroxidase injection for correlation of light and electron microscopic anatomy with synaptic physiology of cultured mouse spinal cord neurons. Brain Res, 152(2), 265-82, 1978.

Nelson, P G, Neale, E A, Macdonald, R L. Formation and modification of synapses in central nervous system cell cultures. Fed Proc, 37(7), 2010-5, 1978.

Macdonald, R L, Barker, J L. Pentylenetetrazol and penicillin are selective antagonists of GABA-mediated post-synaptic inhibition in cultured mammalian neurones. Nature, 267(5613), 720-1, 1977.

Macdonald, R L, Barker, J L. Phenobarbital enhances GABA-mediated postsynaptic inhibition in cultured mammalian neurons. Trans Am Neurol Assoc, 102, 139-40, 1977.

Cohen, D H, MacDonald, R L. Involvement of the avian hypothalamus in defensively conditioned heart rate change. J Comp Neurol, 167(4), 465-80, 1976.

MacDonald, R L, Hsu, D, Mann, J E, Sperelakis, N. An analysis of the problem of K-+ accumulation in the intercalated disk clefts of cardiac muscle. J Theor Biol, 51(2), 455-73, 1975.

Macdonald, R L, Mann, J E, Sperelakis, N. Derivation of general equations describing tracer diffusion in any two-compartment tissue with application to ionic diffusion in cylindrical muscle bundles. J Theor Biol, 45(1), 107-30, 1974.

Sperelakis, N, Macdonald, R L. Ratio of transverse to longitudinal resistivities of isolated cardiac muscle fiber bundles. J Electrocardiol, 7(4), 301-14, 1974.

Macdonald, R L, Cohen, D H. Heart rate and blood pressure responses to electrical stimulation of the central nervous system in the pigeon (Columba livia). J Comp Neurol, 150(2), 109-36, 1973.

Cohen, D H, Macdonald, R L. Some variables affecting orienting and conditioned heart-rate responses in the pigeon. J Comp Physiol Psychol, 74(1), 123-33, 1971.

Cohen, D H, Schnall, A M, Macdonald, R L, Pitts, L H. Medullary cells of origin of vagal cardioinhibitory fibers in the pigeon. I. Anatomical studies of peripheral vagus nerve and the dorsal motor nucleus. J Comp Neurol, 140(3), 299-320, 1970.

Macdonald, R L, Cohen, D H. Cells of origin of sympathetic pre- and postganglionic cardioacceleratory fibers in the pigeon. J Comp Neurol, 140(3), 343-58, 1970.


Postdoctoral Position Available
Yes

Postdoctoral Position Details
Postdoctoral positions are available to participate in NIH-funded studies of inhibitory  aminobutyric acid (GABA) type A (GABAA) receptors. GABAA receptor channels are the major inhibitory neurotransmitter receptors in the brain. Reduction of GABAA receptor function produces seizures and generalized epilepsy in animals and man and enhancement of GABAA receptor function has been used to treat seizures. At least five forms of human idiopathic generalized epilepsy have been linked to mutations in the 1, 3,  and 2 GABAA receptor subunits. Alterations in receptor subunit translation, stability and assembly and receptor surface membrane trafficking, neurotransmitter activation, regulation of channel opening and closing of the channels (gating) and desensitization of these mutant channels is under active investigation. GABAA receptor channels are studied using acute transfection of mammalian cells with expression vectors containing receptor subunit cDNAs and subsequent biochemistry or whole cell or single channel recording or from transgenic or knock in mice expressing the mutant subunit. Human mutations are made in relevant receptor channel subunits to determine the basic mechanism of these genetic human epilepsies. We study mutant subunit translation, mRNA stability, degradation and assembly and trafficking of the mutant receptors using protein chemistry (biotinylation and Western blotting), flow cytometry and confocal microscopy (imaging of subcellular and surface expression of fluorescent tagged receptor subunits). The candidate is expected to have first-hand experience with some of these methods, and to be prepared to learn new methods. The successful candidate will join a productive multi-disciplinary laboratory using multiple tools experimental approaches from single channel recording to investigation of transgenic animals to understand the structure, function and biology of GABAA receptors and the pathogenesis of genetic epilepsy associated with GABAA receptor subunit mutations. Competitive salary will be offered based on experience and publication record. To apply for the position, send a description of your research interests, CV, and the names of 3 references to: Robert.macdonald@vanderbilt.edu, or mail to: Dr. Robert L. Macdonald, Department of Neurology, Vanderbilt University Medical Center, 6140 Medical Research Building III, 465 21st Ave, Nashville, TN 37232-8552

Updated Date
12/06/2011



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