Vanderbilt University School of Medicine

Tyska, Matthew John , Ph.D.
Professor of Cell & Developmental Biology

Lab Url:

Phone Number: (615) 936-5461


Tyska, Matthew's picture

Office Address   Mailing Address

3154 MRB III

3154 MRB III 465 21st Ave South 8240

Research Keywords
epithelial cell biology, biophysics, biochemistry, motor protein, actin cytoskeleton, motility, cell polarity, knockout mouse, cell culture, proteomics, confocal, TIRF, deconvolution, super-resolution, microscopy, live cell imaging, fluorescence, optical tweezers.,Bacteria,Biochemistry,Developmental biology,Enzyme action,Infectious disease,Knockout,Mass spectroscopy,Membrane,Microbiology,Mouse,Mutation,Pathology,Protein Structure,Proteomics ,Bacteria,Biochemistry,Developmental biology,Enzyme action,Infectious disease,Knockout,Mass spectroscopy,Membrane,Microbiology,Mouse,Mutation,Pathology,Protein Structure,Proteomics

Research Description
Research in our laboratory is focused on investigating the architecture, dynamics, and function of the actin cytoskeleton. The context for our studies is the brush border: an array of actin-based protrusions known as microvilli, which extend from the surface of polarized epithelial cells. In the gut, the brush border serves as the sole site of nutrient absorption and a barrier to micro-organisms that reside in the lumenal space. Using an approach that combines biophysics, biochemistry, and cell biology, we are currently studying mechanisms that control microvillar dynamics, morphology, and function. We have also begun to dissect the mechanism of microvillar assembly, which was jumpstarted by our recent elucidation of the entire brush border proteome. A broad long-term goal is to develop our understanding of molecules and pathways that may be perturbed in GI diseases characterized by loss of the brush border (e.g. enteropathogenic E. coli infection, celiac disease, and microvillus inclusion disease).

Crawley, SW, Mooseker, MS, Tyska, MJ. Shaping the intestinal brush border. J Cell Biol, 207(4), 441-51, 2014.

Crawley, SW, Shifrin, DA, Grega-Larson, NE, McConnell, RE, Benesh, AE, Mao, S, Zheng, Y, Zheng, QY, Nam, KT, Millis, BA, Kachar, B, Tyska, MJ. Intestinal brush border assembly driven by protocadherin-based intermicrovillar adhesion. Cell, 157(2), 433-46, 2014.

G??rard, A, Patino-Lopez, G, Beemiller, P, Nambiar, R, Ben-Aissa, K, Liu, Y, Totah, FJ, Tyska, MJ, Shaw, S, Krummel, MF. Detection of rare antigen-presenting cells through T cell-intrinsic meandering motility, mediated by Myo1g. Cell, 158(3), 492-505, 2014.

Knowles, BC, Roland, JT, Krishnan, M, Tyska, MJ, Lapierre, LA, Dickman, PS, Goldenring, JR, Shub, MD. Myosin Vb uncoupling from RAB8A and RAB11A elicits microvillus inclusion disease. J Clin Invest, 124(7), 2947-62, 2014.

Knowles, BC, Tyska, MJ, Goldenring, JR. Apical vesicle trafficking takes center stage in neonatal enteropathies. Gastroenterology, 147(1), 15-7, 2014.

Mazerik, JN, Kraft, LJ, Kenworthy, AK, Tyska, MJ. Motor and tail homology 1 (Th1) domains antagonistically control myosin-1 dynamics. Biophys J, 106(3), 649-58, 2014.

Shifrin, DA, Crawley, SW, Grega-Larson, NE, Tyska, MJ. Dynamics of brush border remodeling induced by enteropathogenic E. coli. Gut Microbes, 5(4), 504-16, 2014.

Hoshino, D, Kirkbride, KC, Costello, K, Clark, ES, Sinha, S, Grega-Larson, N, Tyska, MJ, Weaver, AM. Exosome secretion is enhanced by invadopodia and drives invasive behavior. Cell Rep, 5(5), 1159-68, 2013.

Shifrin, DA, Beckler, MD, Coffey, RJ, Tyska, MJ. Extracellular vesicles: communication, coercion, and conditioning. Mol Biol Cell, 24(9), 1253-9, 2013.

Benesh, AE, Fleming, JT, Chiang, C, Carter, BD, Tyska, MJ. Expression and localization of myosin-1d in the developing nervous system. Brain Res, 1440, 9-22, 2012.

Liu, Y, Belkina, NV, Park, C, Nambiar, R, Loughhead, SM, Patino-Lopez, G, Ben-Aissa, K, Hao, JJ, Kruhlak, MJ, Qi, H, von Andrian, UH, Kehrl, JH, Tyska, MJ, Shaw, S. Constitutively active ezrin increases membrane tension, slows migration, and impedes endothelial transmigration of lymphocytes in vivo in mice. Blood, 119(2), 445-53, 2012.

Mazerik, JN, Tyska, MJ. Myosin-1A targets to microvilli using multiple membrane binding motifs in the tail homology 1 (TH1) domain. J Biol Chem, 287(16), 13104-15, 2012.

Pyrpassopoulos, S, Feeser, EA, Mazerik, JN, Tyska, MJ, Ostap, EM. Membrane-bound myo1c powers asymmetric motility of actin filaments. Curr Biol, 22(18), 1688-92, 2012.

Shifrin, DA, McConnell, RE, Nambiar, R, Higginbotham, JN, Coffey, RJ, Tyska, MJ. Enterocyte microvillus-derived vesicles detoxify bacterial products and regulate epithelial-microbial interactions. Curr Biol, 22(7), 627-31, 2012.

Shifrin, DA, Tyska, MJ. Ready-aim-fire into the lumen: A new role for enterocyte microvilli in gut host defense. Gut Microbes, 3(5), , 2012.

Higginbotham, JN, Demory Beckler, M, Gephart, JD, Franklin, JL, Bogatcheva, G, Kremers, GJ, Piston, DW, Ayers, GD, McConnell, RE, Tyska, MJ, Coffey, RJ. Amphiregulin exosomes increase cancer cell invasion. Curr Biol, 21(9), 779-86, 2011. PMCID:3131266

McConnell, RE, Benesh, AE, Mao, S, Tabb, DL, Tyska, MJ. Proteomic analysis of the enterocyte brush border. Am J Physiol Gastrointest Liver Physiol, , , 2011.

Pyburn, TM, Bensing, BA, Xiong, YQ, Melancon, BJ, Tomasiak, TM, Ward, NJ, Yankovskaya, V, Oliver, KM, Cecchini, G, Sulikowski, GA, Tyska, MJ, Sullam, PM, Iverson, TM. A structural model for binding of the serine-rich repeat adhesin GspB to host carbohydrate receptors. PLoS Pathog, 7(7), e1002112, 2011. PMCID:3131266

Benesh, AE, Nambiar, R, McConnell, RE, Mao, S, Tabb, DL, Tyska, MJ. Differential localization and dynamics of class I myosins in the enterocyte microvillus. Mol Biol Cell, 21(6), 970-8, 2010. PMCID:2836977

McConnell, RE, Tyska, MJ. Leveraging the membrane - cytoskeleton interface with myosin-1. Trends Cell Biol, 20(7), 418-26, 2010. PMCID:2881245

Nambiar, R, McConnell, RE, Tyska, MJ. Myosin motor function: the ins and outs of actin-based membrane protrusions. Cell Mol Life Sci, 67(8), 1239-54, 2010. PMCID:2881245

Tyska, MJ, Nambiar, R. Myosin-1a: A motor for microvillar membrane movement and mechanics. Commun Integr Biol, 3(1), 64-6, 2010. PMCID:2881245

McConnell, RE, Higginbotham, JN, Shifrin, DA, Tabb, DL, Coffey, RJ, Tyska, MJ. The enterocyte microvillus is a vesicle-generating organelle. J Cell Biol, 185(7), 1285-98, 2009. PMCID:2712962

Nambiar, R, McConnell, RE, Tyska, MJ. Control of cell membrane tension by myosin-I. Proc Natl Acad Sci U S A, 106(29), 11972-7, 2009. PMCID:2715533

Wang, J, Qin, R, Ma, Y, Wu, H, Peters, H, Tyska, M, Shaheen, NJ, Chen, X. Differential gene expression in normal esophagus and Barrett''s esophagus. J Gastroenterol, , , 2009.

Yengo, CM, Ananthanarayanan, SK, Brosey, CA, Mao, S, Tyska, MJ. Human deafness mutation E385D disrupts the mechanochemical coupling and subcellular targeting of myosin-1a. Biophys J, 94(2), L5-7, 2008. PMCID:2157235

McConnell, RE, Tyska, MJ. Myosin-1a powers the sliding of apical membrane along microvillar actin bundles. J Cell Biol, 177(4), 671-81, 2007. PMCID:2064212

O''Connell, CB, Tyska, MJ, Mooseker, MS. Myosin at work: motor adaptations for a variety of cellular functions. Biochim Biophys Acta, 1773(5), 615-30, 2007.

Tyska, MJ, Mackey, AT, Huang, JD, Copland, NG, Jenkins, NA, Mooseker, MS. Myosin-1a Is Critical for Normal Brush Border Structure and Composition. Mol Biol Cell, 16, 2443-2457, 2005. PMCID:1087248

Tyska, M J, Mooseker, M S. A role for myosin-1A in the localization of a brush border disaccharidase. J Cell Biol, 165(3), 395-405, 2004. PMCID:2172191

Tyska, M J, Mooseker, M S. Myosin-V motility: these levers were made for walking. Trends Cell Biol, 13(9), 447-51, 2003.

*Post, P L, *Tyska, M J, OConnell, C B, Johung, K, Hayward, A, Mooseker, M S. Myosin-IXb is a single-headed and processive motor. J Biol Chem, 277(14), 11679-83, 2002 *These authors contributed equally to this work..

Tyska, M J, Mooseker, M S. MYO1A (brush border myosin I) dynamics in the brush border of LLC-PK1-CL4 cells. Biophys J, 82(4), 1869-83, 2002. PMCID:1301984

Tyska, M J, Warshaw, D M. The myosin power stroke. Cell Motil Cytoskeleton, 51(1), 1-15, 2002.

Reck-Peterson, S L, Tyska, M J, Novick, P J, Mooseker, M S. The yeast class V myosins, Myo2p and Myo4p, are nonprocessive actin-based motors. J Cell Biol, 153(5), 1121-6, 2001. PMCID:2174330

Palmiter, K A, Tyska, M J, Haeberle, J R, Alpert, N R, Fananapazir, L, Warshaw, D M. R403Q and L908V mutant beta-cardiac myosin from patients with familial hypertrophic cardiomyopathy exhibit enhanced mechanical performance at the single molecule level. J Muscle Res Cell Motil, 21(7), 609-20, 2000.

Tyska, M J, Hayes, E, Giewat, M, Seidman, C E, Seidman, J G, Warshaw, D M. Single-molecule mechanics of R403Q cardiac myosin isolated from the mouse model of familial hypertrophic cardiomyopathy. Circ Res, 86(7), 737-44, 2000.

Warshaw, D M, Guilford, W H, Freyzon, Y, Krementsova, E, Palmiter, K A, Tyska, M J, Baker, J E, Trybus, K M. The light chain binding domain of expressed smooth muscle heavy meromyosin acts as a mechanical lever. J Biol Chem, 275(47), 37167-72, 2000.

Yamashita, H, Tyska, M J, Warshaw, D M, Lowey, S, Trybus, K M. Functional consequences of mutations in the smooth muscle myosin heavy chain at sites implicated in familial hypertrophic cardiomyopathy. J Biol Chem, 275(36), 28045-52, 2000.

Palmiter, K A, Tyska, M J, Dupuis, D E, Alpert, N R, Warshaw, D M. Kinetic differences at the single molecule level account for the functional diversity of rabbit cardiac myosin isoforms. J Physiol, 519 Pt 3, 669-78, 1999. PMCID:2269540

Tyska, M J, Dupuis, D E, Guilford, W H, Patlak, J B, Waller, G S, Trybus, K M, Warshaw, D M, Lowey, S. Two heads of myosin are better than one for generating force and motion. Proc Natl Acad Sci U S A, 96(8), 4402-7, 1999. PMCID:16344

Lauzon, A M, Tyska, M J, Rovner, A S, Freyzon, Y, Warshaw, D M, Trybus, K M. A 7-amino-acid insert in the heavy chain nucleotide binding loop alters the kinetics of smooth muscle myosin in the laser trap. J Muscle Res Cell Motil, 19(8), 825-37, 1998.

Postdoctoral Position Available

Postdoctoral Position Details
A postdoctoral position is available to investigate the function of actin-based motor proteins (myosins) using state-of-the-art cell biological and biophysical approaches. Potential projects include but are not limited to: 1) the mechanical characterization of myosin motors in vitro and in living cells using optical trapping technology, 2) studying the dynamics of the actin cytoskeleton and associated myosins using the latest live cell imaging technologies, and 3) applying advanced proteomic approaches to define the composition of native myosin/cargo complexes and F-actin arrays. Previous experience with cell culture techniques, molecular biology (cloning/mutagenesis), protein biochemistry (expression/purification), and light microscopy is very important. Interested candidates should email a copy of their CV and the names of three references to

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