Vanderbilt University School of Medicine

Manning, H. Charles , Ph.D.
Professor of Radiology and Radiological Sciences
Associate Professor of Chemistry
Associate Professor of Neurological Surgery
Ingram Associate Professor of Cancer Research
Associate Professor of Biomedical Engineering

Lab Url:

Phone Number: (615) 322-3793


Manning, H.'s picture

Office Address   Mailing Address

AAA-3115 Medical Center North/VUIIS

1161 21st Avenue South MCN AA-1105 37232-2310

Research Keywords
Molecular imaging, angiogenesis, imaging probe discovery/development, high-throughput screening (HTS),Apoptosis,Cancer,Cell cycle,Molecular medicine,Neuroscience

Research Specialty
Molecular imaging, chemistry, small-molecule discovery, therapeutic efficacy screening, clinical trial modeling, imaging probe development, imaging validation

Research Description
Our multi-disciplinary research program involves the development, validation, and translation of non-invasive molecular imaging metrics to assess disease progression and response to therapy. Projects within our program emphasize molecular imaging of cancer, and notably imaging-based assessment of response to anti-cancer pharmaceuticals in colorectal and breast cancer. We are actively engaged in advancing novel methods to image molecular events in both humans and animal models that include receptor expression, proliferation, angiogenesis, and apoptosis. Among these methods include validation of imaging probes for novel applications, as well as high-throughput small-molecule discovery to develop novel imaging compounds.

Cheung, YY, Nickels, ML, Tang, D, Buck, JR, Manning, HC. Facile synthesis of SSR180575 and discovery of 7-chloro-N,N,5-trimethyl-4-oxo-3(6-[(18)F]fluoropyridin-2-yl)-3,5-dihydro-4H-pyridazino[4,5-b]indole-1-acetamide, a potent pyridazinoindole ligand for PET imaging of TSPO in cancer. Bioorg Med Chem Lett, 24(18), 4466-71, 2014.

Han, W, Zaynagetdinov, R, Yull, FE, Polosukhin, VV, Gleaves, LA, Tanjore, H, Young, LR, Peterson, TE, Manning, HC, Prince, LS, Blackwell, TS. Molecular Imaging of Folate Receptor Beta Positive Macrophages During Acute Lung Inflammation. Am J Respir Cell Mol Biol, , , 2014.

Hight, MR, Cheung, YY, Nickels, ML, Dawson, ES, Zhao, P, Saleh, S, Buck, JR, Tang, D, Washington, MK, Coffey, RJ, Manning, HC. A Peptide-based positron emission tomography probe for in vivo detection of caspase activity in apoptotic cells. Clin Cancer Res, 20(8), 2126-35, 2014.

McKinley, ET, Zhao, P, Coffey, RJ, Washington, MK, Manning, HC. 3''-Deoxy-3''-[18F]-Fluorothymidine PET imaging reflects PI3K-mTOR-mediated pro-survival response to targeted therapy in colorectal cancer. PLoS One, 9(9), e108193, 2014.

Perrone, MG, Malerba, P, Uddin, MJ, Vitale, P, Panella, A, Crews, BC, Daniel, CK, Ghebreselasie, K, Nickels, M, Tantawy, MN, Manning, HC, Marnett, LJ, Scilimati, A. PET radiotracer [(18)F]-P6 selectively targeting COX-1 as a novel biomarker in ovarian cancer: Preliminary investigation. Eur J Med Chem, 80C, 562-568, 2014.

Powell, AE, Vlacich, G, Zhao, ZY, McKinley, ET, Washington, MK, Manning, HC, Coffey, RJ. Inducible Loss of One Apc Allele in Lrig1-Expressing Progenitor Cells Results in Multiple Distal Colonic Tumors with Features of Familial Adenomatous Polyposis. Am J Physiol Gastrointest Liver Physiol, , , 2014.

Tang, D, Nickels, ML, Tantawy, MN, Buck, JR, Manning, HC. Preclinical Imaging Evaluation of Novel TSPO-PET Ligand 2-(5,7-Diethyl-2-(4-(2-[(18)F]fluoroethoxy)phenyl)pyrazolo[1,5-a]pyrimidin-3-yl)-N,N-diethylacetamide ([ (18)F]VUIIS1008) in Glioma. Mol Imaging Biol, , , 2014.

Uddin, MI, Buck, JR, Schulte, ML, Tang, D, Saleh, SA, Cheung, YY, Harp, J, Manning, HC. Microwave-assisted, one-pot reaction of 7-azaindoles and aldehydes: a facile route to novel di-7-azaindolylmethanes. Tetrahedron Lett, 55(1), , 2014.

Whisenant, JG, McIntyre, JO, Peterson, TE, Kang, H, S??nchez, V, Manning, HC, Arteaga, CL, Yankeelov, TE. Utility of [(18)???F]FLT-PET to Assess Treatment Response in Trastuzumab-Resistant and Trastuzumab-Sensitive HER2-Overexpressing Human Breast Cancer Xenografts. Mol Imaging Biol, , , 2014.

Katsha, A, Soutto, M, Sehdev, V, Peng, D, Washington, MK, Piazuelo, MB, Tantawy, MN, Manning, HC, Lu, P, Shyr, Y, Ecsedy, J, Belkhiri, A, El-Rifai, W. Aurora Kinase A Promotes Inflammation and Tumorigenesis in Mice and Human Gastric Neoplasia. Gastroenterology, , , 2013.

McKinley, ET, Ayers, GD, Smith, RA, Saleh, SA, Zhao, P, Washington, MK, Coffey, RJ, Manning, HC. Limits of [18F]-FLT PET as a biomarker of proliferation in oncology. PLoS One, 8(3), e58938, 2013.

McKinley, ET, Liu, H, McDonald, WH, Luo, W, Zhao, P, Coffey, RJ, Hanks, SK, Manning, HC. Global phosphotyrosine proteomics identifies PKC?? as a marker of responsiveness to Src inhibition in colorectal cancer. PLoS One, 8(11), e80207, 2013.

McKinley, ET, Smith, RA, Zhao, P, Fu, A, Saleh, SA, Uddin, MI, Washington, MK, Coffey, RJ, Manning, HC. 3''-Deoxy-3''-18F-fluorothymidine PET predicts response to (V600E)BRAF-targeted therapy in preclinical models of colorectal cancer. J Nucl Med, 54(3), 424-30, 2013.

Tang, D, McKinley, ET, Hight, MR, Uddin, MI, Harp, JM, Fu, A, Nickels, ML, Buck, JR, Manning, HC. Synthesis and Structure-Activity Relationships of 5,6,7-Substituted Pyrazolopyrimidines: Discovery of a Novel TSPO PET Ligand for Cancer Imaging. J Med Chem, 56(8), 3429-33, 2013.

Thompson, MM, Manning, HC, Ellacott, KL. Translocator protein 18 kDa (TSPO) is regulated in white and brown adipose tissue by obesity. PLoS One, 8(11), e79980, 2013.

Walsh, AJ, Cook, RS, Manning, HC, Hicks, DJ, Lafontant, A, Arteaga, CL, Skala, MC. Optical metabolic imaging identifies glycolytic levels, subtypes, and early-treatment response in breast cancer. Cancer Res, 73(20), 6164-74, 2013.

Xie, J, Wang, C, Virostko, J, Manning, HC, Pham, W, Bauer, J, Gore, JC. A novel reporter system for molecular imaging and high-throughput screening of anticancer drugs. Chembiochem, 14(12), 1494-503, 2013.

Buck, JR, Saleh, S, Uddin, MI, Manning, HC. Rapid, Microwave-Assisted Organic Synthesis of Selective (V600E)BRAF Inhibitors for Preclinical Cancer Research. Tetrahedron Lett, 53(32), 4161-4165, 2012.

McKinley, ET, Smith, RA, Tanksley, JP, Washington, MK, Walker, R, Coffey, RJ, Manning, HC. [(18)F]FLT-PET to predict pharmacodynamic and clinical response to cetuximab therapy in M??n??trier''s disease. Ann Nucl Med, 26(9), 757-63, 2012.

Myers, MV, Manning, HC, Coffey, RJ, Liebler, DC. Protein expression signatures for inhibition of epidermal growth factor receptor-mediated signaling. Mol Cell Proteomics, 11(2), M111.015222, 2012. PMCID:3277773

Tang, D, Hight, MR, McKinley, ET, Fu, A, Buck, JR, Smith, RA, Tantawy, MN, Peterson, TE, Colvin, DC, Ansari, MS, Nickels, M, Manning, HC. Quantitative Preclinical Imaging of TSPO Expression in Glioma Using N,N-Diethyl-2-(2-(4-(2-18F-Fluoroethoxy)Phenyl)-5,7-Dimethylpyrazolo[1,5-a]Pyrimidin-3-yl)Acetamide. J Nucl Med, 53(2), 287-94, 2012.

Wyatt, SK, Manning, HC, Bai, M, Ehtesham, M, Mapara, KY, Thompson, RC, Bornhop, DJ. Preclinical molecular imaging of the translocator protein (TSPO) in a metastases model based on breast cancer xenografts propagated in the murine brain. Curr Mol Med, 12(4), 458-66, 2012.

Xu, J, Li, K, Smith, RA, Waterton, JC, Zhao, P, Chen, H, Does, MD, Manning, HC, Gore, JC. Characterizing tumor response to chemotherapy at various length scales using temporal diffusion spectroscopy. PLoS One, 7(7), e41714, 2012.

Yankeelov, TE, Peterson, TE, Abramson, RG, Garcia-Izquierdo, D, Arlinghaus, LR, Li, X, Atuegwu, NC, Catana, C, Manning, HC, Fayad, ZA, Gore, JC. Simultaneous PET-MRI in oncology: a solution looking for a problem. Magn Reson Imaging, 30(9), 1342-56, 2012.

Buck, JR, McKinley, ET, Hight, MR, Fu, A, Tang, D, Smith, RA, Tantawy, MN, Peterson, TE, Colvin, D, Ansari, MS, Baldwin, RM, Zhao, P, Guleryuz, S, Manning, HC. Quantitative, Preclinical PET of Translocator Protein Expression in Glioma Using 18F-N-Fluoroacetyl-N-(2,5-Dimethoxybenzyl)-2-Phenoxyaniline. J Nucl Med, 52(1), 107-14, 2011.

Fox, EM, Miller, TW, Balko, JM, Kuba, MG, S?!nchez, V, Smith, RA, Liu, S, Gonz?!lez-Angulo, AM, Mills, GB, Ye, F, Shyr, Y, Manning, HC, Buck, E, Arteaga, CL. A kinome-wide screen identifies the insulin/IGF-I receptor pathway as a mechanism of escape from hormone dependence in breast cancer. Cancer Res, 71(21), 6773-84, 2011. PMCID:3206206

Garrett, JT, Olivares, MG, Rinehart, C, Granja-Ingram, ND, S?!nchez, V, Chakrabarty, A, Dave, B, Cook, RS, Pao, W, McKinely, E, Manning, HC, Chang, J, Arteaga, CL. Transcriptional and posttranslational up-regulation of HER3 (ErbB3) compensates for inhibition of the HER2 tyrosine kinase. Proc Natl Acad Sci U S A, 108(12), 5021-6, 2011. PMCID:3064360

Gore, JC, Manning, HC, Quarles, CC, Waddell, KW, Yankeelov, TE. Magnetic resonance in the era of molecular imaging of cancer. Magn Reson Imaging, 29(5), 587-600, 2011. PMCID:3206206

Guo, N, Xie, J, Manning, HC, Deane, NG, Ansari, MS, Coffey, RJ, Gore, J, Price, RR, Baldwin, RM, McIntyre, JO. A novel in vitro assay to assess phosphorylation of 3''-[(18)F]fluoro-3''-deoxythymidine. Mol Imaging Biol, 13(2), 257-64, 2011. PMCID:2913893

Hight, MR, Nolting, DD, McKinley, ET, Lander, AD, Wyatt, SK, Gonyea, M, Zhao, P, Manning, HC. Multispectral fluorescence imaging to assess pH in biological specimens. J Biomed Opt, 16(1), 016007, 2011. PMCID:3041815

McKinley, ET, Bugaj, JE, Zhao, P, Guleryuz, S, Mantis, C, Gokhale, PC, Wild, R, Manning, HC. 18FDG-PET predicts pharmacodynamic response to OSI-906, a dual IGF-1R/IR inhibitor, in preclinical mouse models of lung cancer. Clin Cancer Res, 17(10), 3332-40, 2011. PMCID:3122480

Miller, TW, Balko, JM, Fox, EM, Ghazoui, Z, Dunbier, A, Anderson, H, Dowsett, M, Jiang, A, Smith, RA, Maira, SM, Manning, HC, Gonz?!lez-Angulo, AM, Mills, GB, Higham, C, Chanthaphaychith, S, Kuba, MG, Miller, WR, Shyr, Y, Arteaga, CL. ERI?-dependent E2F transcription can mediate resistance to estrogen deprivation in human breast cancer. Cancer Discov, 1(4), 338-351, 2011. PMCID:3204388

Smith, RA, Guleryuz, S, Manning, HC. Molecular imaging metrics to evaluate response to preclinical therapeutic regimens. Front Biosci, 16, 393-410, 2011.

Ayers, GD, McKinley, ET, Zhao, P, Fritz, JM, Metry, RE, Deal, BC, Adlerz, KM, Coffey, RJ, Manning, HC. Volume of preclinical xenograft tumors is more accurately assessed by ultrasound imaging than manual caliper measurements. J Ultrasound Med, 29(6), 891-901, 2010.

Tang, D, Buck, JR, Hight, MR, Manning, HC. Microwave-assisted Organic Synthesis of a High-affinity Pyrazolo-pyrimidinyl TSPO Ligand. Tetrahedron Lett, 51(35), 4595-4598, 2010.

Manning, HC, Shay, SD, Mericle, RA. Multispectral molecular imaging of capillary endothelium to facilitate preoperative endovascular brain mapping. J Neurosurg, , , 2009.

Miller, TW, Forbes, JT, Shah, C, Wyatt, SK, Manning, HC, Olivares, MG, Sanchez, V, Dugger, TC, de Matos Granja, N, Narasanna, A, Cook, RS, Kennedy, JP, Lindsley, CW, Arteaga, CL. Inhibition of mammalian target of rapamycin is required for optimal antitumor effect of HER2 inhibitors against HER2-overexpressing cancer cells. Clin Cancer Res, 15(23), 7266-76, 2009.

Peterson, TE, Manning, HC. Molecular imaging: 18F-FDG PET and a whole lot more. J Nucl Med Technol, 37(3), 151-61, 2009.

Sexton, M, Woodruff, G, Cudaback, E, Kreitzer, FR, Xu, C, Lin, YH, M??ller, T, Bai, M, Manning, HC, Bornhop, D, Stella, N. Binding of NIR-conPK and NIR-6T to Astrocytomas and Microglial Cells: Evidence for a Protein Related to TSPO. PLoS One, 4(12), e8271, 2009. PMCID:2792720

Shah, C, Miller, TW, Wyatt, SK, McKinley, ET, Olivares, MG, Sanchez, V, Nolting, DD, Buck, JR, Zhao, P, Ansari, MS, Baldwin, RM, Gore, JC, Schiff, R, Arteaga, CL, Manning, HC. Imaging biomarkers predict response to anti-HER2 (ErbB2) therapy in preclinical models of breast cancer. Clin Cancer Res, 15(14), 4712-21, 2009.

Virostko, J, Xie, J, Hallahan, DE, Arteaga, CL, Gore, JC, Manning, HC. A Molecular Imaging Paradigm to Rapidly Profile Response to Angiogenesis-directed Therapy in Small Animals. Mol Imaging Biol, , , 2009. PMCID:2677126

Wyatt, SK, Manning, HC, Bai, M, Bailey, SN, Gallant, P, Ma, G, McIntosh, L, Bornhop, DJ. Molecular Imaging of the Translocator Protein (TSPO) in a Pre-Clinical Model of Breast Cancer. Mol Imaging Biol, , , 2009.

Yankeelov, TE, Avison, MJ, Damon, BM, Manning, HC, Peterson, TE, Gore, JC. Frontiers of Biomedical Imaging Science 2009: workshop report and research opportunities. Cancer Res, 69(20), 7902-4, 2009.

H. Charles Manning. Optical imaging shines in vivo. Journal of Nuclear Medicine, 49, 23N-29N, 2008.

Manning, HC, Lander, A, McKinley, E, Mutic, NJ. Accelerating the Development of Novel Molecular Imaging Probes: A Role for High-Throughput Screening. J Nucl Med, , , 2008. PMCID:2576283

Manning, HC, Merchant, NB, Foutch, AC, Virostko, JM, Wyatt, SK, Shah, C, McKinley, ET, Xie, J, Mutic, NJ, Washington, MK, Lafleur, B, Tantawy, MN, Peterson, TE, Ansari, MS, Baldwin, RM, Rothenberg, ML, Bornhop, DJ, Gore, JC, Coffey, RJ. Molecular Imaging of Therapeutic Response to Epidermal Growth Factor Receptor Blockade in Colorectal Cancer. Clin Cancer Res, 14(22), 7413-7422, 2008. PMCID:2657180

Deane, NG, Manning, HC, Foutch, AC, Washington, MK, Aronow, BA, Bornhop, DJ, Coffey, RJ. Targeted imaging of colonic tumors in smad3-/- mice discriminates cancer and inflammation. Mol Cancer Res, 5(4), 341-9, 2007.

Manning, HC, Smith, SM, Sexton, M, Haviland, S, Bai, M, Cederquist, K, Stella, N, Bornhop, DJ. A peripheral benzodiazepine receptor targeted agent for in vitro imaging and screening. Bioconjug Chem, 17(3), 735-40, 2006.

Manning, HC., Bai, M., Anderson, BM., Lisiak, R., Samuelson, L., Bornhop, D.J. Expeditious synthesis of P-Protected macrocycles in-route to lanthanide chelate metal complexes. Tetrahedron Letters, 46, 4707-4710, 2005.

Smentek, L, Andes Hess, B, Cross, JP, Charles Manning, H, Bornhop, DJ. Density-functional theory structures of 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid complexes for ions across the lanthanide series. J Chem Phys, 123(24), 244302, 2005.

Manning, HC, Goebel, T, Thompson, RC, Price, RR, Lee, H, Bornhop, DJ. Targeted molecular imaging agents for cellular-scale bimodal imaging. Bioconjug Chem, 15(6), 1488-95, 2004.

Manning, HC., Skwierawska, A., Marx, JN., et al. Rapid removal of benzyloxycarbonyl groups from 1,4,7,10-tetraazacyclododecane derivatives by catalytic transfer hydrogenation. Synthetic Communications, 33(3), 457-461, 2003.

Marcu, L., Vernier, T., Manning, H.C., Salemi, S., Li, A., Craft, C., Gundersen, M., Bornhop, D. Fluorescence microscopy studies of a peripheral-benzodiazepine-receptor-targeted molecular probe for brain tumor imaging.. Proceedings of SPIE-The International Society for Optical Engineering, 5141, 64-69, 2003.

Manning, HC, Goebel, T, Marx, JN, Bornhop, DJ. Facile, efficient conjugation of a trifunctional lanthanide chelate to a peripheral benzodiazepine receptor ligand. Org Lett, 4(7), 1075-8, 2002.

Griffin, JMM., Skwierawska, AM., Manning, HC., Marx, JN., Bornhop, DJ. Simple, high-yielding synthesis of trifunctional fluorescent lanthanide chelates. Tetrahedron Letters, 42, 3823-3825, 2001.

Postdoctoral Position Available

Postdoctoral Position Details
Accepting applications for trainees interested in imaging science. Contact H. Charles Manning, Ph.D. for more details. (

Updated Date

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