Vanderbilt University School of Medicine

Lim, Chee C. , Ph.D.
Assistant Professor of Medicine
Assistant Professor of Molecular Physiology and Biophysics

Lab Url: http://www.vanderbilthealth.com/cardiovascular/23004?previewsession=1

Phone Number: 615-936-2510

Email Address:chee.lim@vanderbilt.edu

Lim, Chee's picture

Office Address   Mailing Address

332 Preston Research Building

2220 Pierce Avenue, Room 332 PRB Vanderbilt University Medical Center 37232


Research Keywords
cardiac biology, physiology, transcription, gene regulation

Research Specialty
Cardiac biology and cardiomyocyte mechanics

Research Description
The over-riding research theme in our lab is to understand the mechanisms that regulate cardiac sarcomere turnover. The cardiac sarcomere is a complex and highly ordered ensemble of contractile and regulatory proteins designed to generate force. To maintain functional sarcomeres, precise turnover of proteins is required that balances new protein synthesis and incorporation into the sarcomere with removal and degradation of worn out or damaged proteins. Titin is a giant elastic protein, spanning half a sarcomere, and forms the structural a??backbonea?? of the sarcomere; yet, the mechanisms regulating the turnover of titin are completely unknown. The goal of our lab is to use an integrative molecular biology approach to evaluate a number of key aspects in a model we have proposed for titin turnover.
Our lab employs a wide range of experimental techniques in molecular biology, physiology, and bioinformatics. Experimental model systems used in our lab include neonatal and adult cardiomyocytes, isolated Langendorff-perfused hearts, mouse myocardial infarction, and genetically modified mouse models.

Publications
Safa, RN, Peng, XY, Pentassuglia, L, Lim, CC, Lamparter, M, Silverstein, C, Walker, J, Chen, B, Geisberg, C, Hatzopoulos, AK, Sawyer, DB. Neuregulin-1I? regulation of embryonic endothelial progenitor cell survival. Am J Physiol Heart Circ Physiol, 300(4), H1311-9, 2011.

Kuster, GM, Lancel, S, Zhang, J, Communal, C, Trucillo, MP, Lim, CC, Pfister, O, Weinberg, EO, Cohen, RA, Liao, R, Siwik, DA, Colucci, WS. Redox-mediated reciprocal regulation of SERCA and Na+-Ca2+ exchanger contributes to sarcoplasmic reticulum Ca2+ depletion in cardiac myocytes. Free Radic Biol Med, 48(9), 1182-7, 2010.

Sawyer, DB, Peng, X, Chen, B, Pentassuglia, L, Lim, CC. Mechanisms of anthracycline cardiac injury: can we identify strategies for cardioprotection. Prog Cardiovasc Dis, 53(2), 105-13, 2010.

Jain, M, Jakubowski, A, Cui, L, Shi, J, Su, L, Bauer, M, Guan, J, Lim, CC, Naito, Y, Thompson, JS, Sam, F, Ambrose, C, Parr, M, Crowell, T, Lincecum, JM, Wang, MZ, Hsu, YM, Zheng, TS, Michaelson, JS, Liao, R, Burkly, LC. A novel role for tumor necrosis factor-like weak inducer of apoptosis (TWEAK) in the development of cardiac dysfunction and failure. Circulation, 119(15), 2058-68, 2009.

Lim, CC, Bryan, NS, Jain, M, Garcia-Saura, MF, Fernandez, BO, Sawyer, DB, Handy, DE, Loscalzo, J, Feelisch, M, Liao, R. Glutathione peroxidase deficiency exacerbates ischemia-reperfusion injury in male but not female myocardium: insights into antioxidant compensatory mechanisms. Am J Physiol Heart Circ Physiol, 297(6), H2144-53, 2009.

Perlman, DH, Bauer, SM, Ashrafian, H, Bryan, NS, Garcia-Saura, MF, Lim, CC, Fernandez, BO, Infusini, G, McComb, ME, Costello, CE, Feelisch, M. Mechanistic Insights Into Nitrite-Induced Cardioprotection Using an Integrated Metabonomic/Proteomic Approach. Circ Res, , , 2009.

Lim, CC, Yang, H, Yang, M, Wang, CK, Shi, J, Berg, EA, Pimentel, DR, Gwathmey, JK, Hajjar, RJ, Helmes, M, Costello, CE, Huo, S, Liao, R. A novel mutant cardiac troponin C disrupts molecular motions critical for calcium binding affinity and cardiomyocyte contractility. Biophys J, 94(9), 3577-89, 2008. PMCID:2292379

Zhu, M, Gach, AA, Liu, G, Xu, X, Lim, CC, Zhang, JX, Mao, L, Chuprun, K, Koch, WJ, Liao, R, Koren, G, Blaxall, BC, Mende, U. Enhanced calcium cycling and contractile function in transgenic hearts expressing constitutively active G alpha o* protein. Am J Physiol Heart Circ Physiol, 294(3), H1335-47, 2008.

Chen, B, Peng, X, Pentassuglia, L, Lim, CC, Sawyer, DB. Molecular and cellular mechanisms of anthracycline cardiotoxicity. Cardiovasc Toxicol, 7(2), 114-21, 2007.

Zhao, Y, Lim, CC, Sawyer, DB, Liao, R, Zhang, X. Simultaneous orientation and cellular force measurements in adult cardiac myocytes using three-dimensional polymeric microstructures. Cell Motil Cytoskeleton, 64(9), 718-25, 2007.

Ichinose, F, Buys, ES, Neilan, TG, Furutani, EM, Morgan, JG, Jassal, DS, Graveline, AR, Searles, RJ, Lim, CC, Kaneki, M, Picard, MH, Scherrer-Crosbie, M, Janssens, S, Liao, R, Bloch, KD. Cardiomyocyte-Specific Overexpression of Nitric Oxide Synthase 3 Prevents Myocardial Dysfunction in Murine Models of Septic Shock. Circ Res, , , 2006.

Kuramochi, Y, Guo, X, Sawyer, DB, Lim, CC. Rapid electrical stimulation induces early activation of kinase signal transduction pathways and apoptosis in adult rat ventricular myocytes. Exp Physiol, 91(4), 773-80, 2006.

Yang, M, Lim, CC, Liao, R, Zhang, X. A novel microfluidic impedance assay for monitoring endothelin-induced cardiomyocyte hypertrophy. Biosens Bioelectron, , , 2006.

Yang, M. Lim, C. C. Liao, R. Zhang, X. . Oriented and vectorial patterning of cardiac myocytes using a microfluidic dielectrophoresis chip ? towards engineered cardiac tissue with controlled macroscopic anisotropy.. J Microelectromechanical Systems , 15(6), 1483-1491, 2006.

Zhao Y, Lim CC, Sawyer DB, Liao R, Zhang X. Microchip for subcellular mechanics study in living cells . Sensors and Actuators B: Chemical, 114(2), 1108-1115, 2006.

Lim, CC, Sawyer, DB. Modulation of cardiac function: titin springs into action. J Gen Physiol, 125(3), 249-52, 2005. PMCID:2234017

Peng, X, Chen, B, Lim, CC, Sawyer, DB. The Cardiotoxicology of Anthracycline Chemotherapeutics: TRANSLATING MOLECULAR MECHANISM INTO PREVENTATIVE MEDICINE. Mol Interv, 5(3), 163-71, 2005.

Zhao Y, Lim CC, Sawyer DB, Liao R, Zhang X. Cellular force measurements using single-spaced polymeric microstructure: Isolating cells from base substrate. J. Micromech. Microeng., 15(9), 1649-1656, 2005.

Kuramochi, Y, Lim, CC, Guo, X, Colucci, WS, Liao, R, Sawyer, DB. Myocyte contractile activity modulates norepinephrine cytotoxicity and survival effects of neuregulin-1beta. Am J Physiol Cell Physiol, 286(2), C222-9, 2004.

Lim, CC, Zuppinger, C, Guo, X, Kuster, GM, Helmes, M, Eppenberger, HM, Suter, TM, Liao, R, Sawyer, DB. Anthracyclines induce calpain-dependent titin proteolysis and necrosis in cardiomyocytes. J Biol Chem, 279(9), 8290-9, 2004.

Helmes, M, Lim, CC, Liao, R, Bharti, A, Cui, L, Sawyer, DB. Titin determines the Frank-Starling relation in early diastole. J Gen Physiol, 121(2), 97-110, 2003. PMCID:2217323

Jain, M, Brenner, DA, Cui, L, Lim, CC, Wang, B, Pimentel, DR, Koh, S, Sawyer, DB, Leopold, JA, Handy, DE, Loscalzo, J, Apstein, CS, Liao, R. Glucose-6-phosphate dehydrogenase modulates cytosolic redox status and contractile phenotype in adult cardiomyocytes. Circ Res, 93(2), e9-16, 2003.

Forgione, MA, Cap, A, Liao, R, Moldovan, NI, Eberhardt, RT, Lim, CC, Jones, J, Goldschmidt-Clermont, PJ, Loscalzo, J. Heterozygous cellular glutathione peroxidase deficiency in the mouse: abnormalities in vascular and cardiac function and structure. Circulation, 106(9), 1154-8, 2002.

Jain, M, Lim, CC, Nagata, K, Davis, VM, Milstone, DS, Liao, R, Mortensen, RM. Targeted inactivation of Galpha(i) does not alter cardiac function or beta-adrenergic sensitivity. Am J Physiol Heart Circ Physiol, 280(2), H569-75, 2001.

Lim, CC, Helmes, MH, Sawyer, DB, Jain, M, Liao, R. High-throughput assessment of calcium sensitivity in skinned cardiac myocytes. Am J Physiol Heart Circ Physiol, 281(2), H969-74, 2001.

Lim, CC, Apstein, CS, Colucci, WS, Liao, R. Impaired cell shortening and relengthening with increased pacing frequency are intrinsic to the senescent mouse cardiomyocyte. J Mol Cell Cardiol, 32(11), 2075-82, 2000.

Nagata, K, Communal, C, Lim, CC, Jain, M, Suter, TM, Eberli, FR, Satoh, N, Colucci, WS, Apstein, CS, Liao, R. Altered beta-adrenergic signal transduction in nonfailing hypertrophied myocytes from Dahl salt-sensitive rats. Am J Physiol Heart Circ Physiol, 279(5), H2502-8, 2000.

Saupe, KW, Lim, CC, Ingwall, JS, Apstein, CS, Eberli, FR. Comparison of hearts with 2 types of pressure-overload left ventricular hypertrophy. Hypertension, 35(5), 1167-72, 2000.

Lim, CC, Liao, R, Varma, N, Apstein, CS. Impaired lusitropy-frequency in the aging mouse: role of Ca(2+)-handling proteins and effects of isoproterenol. Am J Physiol, 277(5 Pt 2), H2083-90, 1999.

Shung, KK, Cloutier, G, Lim, CC. The effects of hematocrit, shear rate, and turbulence on ultrasonic Doppler spectrum from blood. IEEE Trans Biomed Eng, 39(5), 462-9, 1992.


Postdoctoral Position Available
No

Postdoctoral Position Details
N/A

Updated Date
09/28/2011



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