Vanderbilt University School of Medicine

Weitkamp, Jorn-Hendrik , M.D.
Assistant Professor of Pediatrics

Lab Url: http://

Phone Number: (615) 343-9297

Email Address:hendrik.weitkamp@vanderbilt.edu

Weitkamp, Jorn-Hendrik's picture

Office Address   Mailing Address

1035H MRB IV / Light Hall

1125 MRB IV / Light Hall 37232-9566


Research Keywords
Gastrointestinal immune development; necrotizing enterocolitis (NEC); FOXP3; immunology; infectious disease; neonatology

Research Description
Necrotizing entercolitis (NEC) is the most common gastrointestinal emergency in premature infants. Immaturity of gastrointestinal host defenses and deregulated immunological and inflammatory responses are major factors in NEC. FOXP3-positive regulatory T cells (Tregs) are specialized CD4+ T cells that regulate innate and adaptive immune responses. Very little data exist on the ontogeny of Tregs in the murine and human gastrointestinal tract.

We developed a reliable and efficient method for the isolation of intestinal lamina propria mononuclear cells from surgically resected intestinal tissue samples from infants with and without NEC. We are using flow cytometry, cytokine and T cell suppression assays, and molecular techniques to analyze phenotypic and functional properties of these cells.

By studying the development of effector and regulatory T cells in human infants, we will gain knowledge about gastrointestinal immune development, its role in neonatal and adult disease pathogenesis and how it is influenced by environmental factors such as nutrition and bacterial colonization.

Clinical Research Description
We are involved in several single center or multi center clinical research projects that are aimed at prevention, early diagnosis, or treatment of neonatal infectious diseases.

Projects include:
- NICHD Meropenem safety and pharmacokinetic trial
- Astellas Mycafungin trial
- Study to assess the role of vertical MRSA colonization
- Silver-alginate dressings to prevent PICC infections
- Machine learning techniques for earl detection of neonatal sepsis

Clinical Interests
Neonatal infectious diseases and immunology
Necrotizing enterocolitis

Publications
Engelhardt, BG, Jagasia, SM, Crowe, JE, Griffith, ML, Savani, BN, Kassim, AA, Lu, P, Weitkamp, JH, Moore, DJ, Yoder, SM, Rock, MT, Jagasia, M. Predicting posttransplantation diabetes mellitus by regulatory T-cell phenotype: implications for metabolic intervention to modulate alloreactivity. Blood, 119(10), 2417-21, 2012.

Mohankumar, K, Kaza, N, Jagadeeswaran, R, Garzon, SA, Bansal, A, Kurundkar, AR, Namachivayam, K, Remon, JI, Bandepalli, R, Feng, X, Weitkamp, JH, Maheshwari, A. Gut Mucosal Injury in Neonates is marked by Macrophage Infiltration in Contrast to Pleomorphic Infiltrates in Adult: Evidence from an Animal Model. Am J Physiol Gastrointest Liver Physiol, , , 2012.

Plosa, EJ, Esbenshade, JC, Fuller, MP, Weitkamp, JH. Cytomegalovirus infection. Pediatr Rev, 33(4), 156-63; quiz 163, 2012.

Romano-Keeler, J, Weitkamp, JH, Moore, DJ. Regulatory properties of the intestinal microbiome effecting the development and treatment of diabetes. Curr Opin Endocrinol Diabetes Obes, 19(2), 73-80, 2012.

Ullrich, T, Tang, YW, Correa, H, Garzon, SA, Maheshwari, A, Hill, M, Matta, P, Krishnan, MK, Weitkamp, JH. Absence of gastrointestinal pathogens in ileum tissue resected for necrotizing enterocolitis. Pediatr Infect Dis J, 31(4), 413-4, 2012.

Weitkamp, JH, Koyama, T, Rock, MT, Correa, H, Goettel, JA, Matta, P, Oswald-Richter, K, Rosen, MJ, Engelhardt, BG, Moore, DJ, Polk, DB. Necrotising enterocolitis is characterised by disrupted immune regulation and diminished mucosal regulatory (FOXP3)/effector (CD4, CD8) T cell ratios. Gut, , , 2012.

Zhang, C, Sherman, MP, Prince, LS, Bader, D, Weitkamp, JH, Slaughter, JC, McElroy, SJ. Paneth cell ablation in the presence of Klebsiella pneumoniae induces necrotizing enterocolitis (NEC)-like injury in the small intestine of immature mice. Dis Model Mech, , , 2012.

Lytkin, NI, McVoy, L, Weitkamp, JH, Aliferis, CF, Statnikov, A. Expanding the understanding of biases in development of clinical-grade molecular signatures: a case study in acute respiratory viral infections. PLoS One, 6(6), e20662, 2011. PMCID:3105991

McElroy, SJ, Prince, LS, Weitkamp, JH, Reese, J, Slaughter, JC, Polk, DB. Tumor necrosis factor receptor 1-dependent depletion of mucus in immature small intestine: a potential role in neonatal necrotizing enterocolitis. Am J Physiol Gastrointest Liver Physiol, 301(4), G656-66, 2011.

Smith, PB, Cohen-Wolkowiez, M, Castro, LM, Poindexter, B, Bidegain, M, Weitkamp, JH, Schelonka, RL, Ward, RM, Wade, K, Valencia, G, Burchfield, D, Arrieta, A, Bhatt-Mehta, V, Walsh, M, Kantak, A, Rasmussen, M, Sullivan, JE, Finer, N, Brozanski, BS, Sanchez, P, van den Anker, J, Blumer, J, Kearns, GL, Capparelli, EV, Anand, R, Benjamin, DK, , . Population pharmacokinetics of meropenem in plasma and cerebrospinal fluid of infants with suspected or complicated intra-abdominal infections. Pediatr Infect Dis J, 30(10), 844-9, 2011.

Halpern, MD, Weitkamp, JH, Mount Patrick, SK, Dobrenen, HJ, Khailova, L, Correa, H, Dvorak, B. Apical sodium-dependent bile acid transporter upregulation is associated with necrotizing enterocolitis. Am J Physiol Gastrointest Liver Physiol, 299(3), G623-31, 2010. PMCID:2950692

Hill, ML, Baldwin, L, Slaughter, JC, Walsh, WF, Weitkamp, JH. A silver-alginate-coated dressing to reduce peripherally inserted central catheter (PICC) infections in NICU patients: a pilot randomized controlled trial. J Perinatol, 30(7), 469-73, 2010. PMCID:2950692

Statnikov, A, Lytkin, NI, McVoy, L, Weitkamp, JH, Aliferis, CF. Using gene expression profiles from peripheral blood to identify asymptomatic responses to acute respiratory viral infections. BMC Res Notes, 3, 264, 2010. PMCID:2975649

Weitkamp, JH, Rudzinski, E, Koyama, T, Correa, H, Matta, P, Alberty, B, Polk, DB. Ontogeny of FOXP3(+) regulatory T cells in the postnatal human small intestinal and large intestinal lamina propria. Pediatr Dev Pathol, 12(6), 443-9, 2009. PMCID:2844857

Williams, JV, Weitkamp, JH, Blum, DL, LaFleur, BJ, Crowe, JE. The human neonatal B cell response to respiratory syncytial virus uses a biased antibody variable gene repertoire that lacks somatic mutations. Mol Immunol, 47(2-3), 407-14, 2009. PMCID:2788105

Weitkamp, JH, Ozdas, A, Lafleur, B, Potts, AL. Fluconazole prophylaxis for prevention of invasive fungal infections in targeted highest risk preterm infants limits drug exposure. J Perinatol, , , 2008.

Sparks, JR, Recchia, FM, Weitkamp, JH. Endogenous group B streptococcal endophthalmitis in a preterm infant. J Perinatol, 27(6), 392-4, 2007.

Choudhury, SA, Berthaud, V, Weitkamp, JH. Meningitis caused by Salmonella panama in infants. J Natl Med Assoc, 98(2), 219-22, 2006. PMCID:2595037

Weitkamp, JH, Kallewaard, NL, Bowen, AL, Lafleur, BJ, Greenberg, HB, Crowe, JE. VH1-46 is the dominant immunoglobulin heavy chain gene segment in rotavirus-specific memory B cells expressing the intestinal homing receptor alpha4beta7. J Immunol, 174(6), 3454-60, 2005.

Weitkamp, JH, Lafleur, BJ, Crowe, JE. Rotavirus-specific CD5+ B cells in young children exhibit a distinct antibody repertoire compared with CD5- B cells. Hum Immunol, 67(1-2), 33-42, 2005.

Weitkamp, JH, Lafleur, BJ, Greenberg, HB, Crowe, JE. Natural evolution of a human virus-specific antibody gene repertoire by somatic hypermutation requires both hotspot-directed and randomly-directed processes. Hum Immunol, 66(6), 666-76, 2005.

Weitkamp, JH, Spring, MD, Brogan, T, Moses, H, Bloch, KC, Wright, PF. Influenza A virus-associated acute necrotizing encephalopathy in the United States. Pediatr Infect Dis J, 23(3), 259-63, 2004.

Weitkamp, JH, Kallewaard, N, Kusuhara, K, Bures, E, Williams, JV, LaFleur, B, Greenberg, HB, Crowe, JE. Infant and adult human B cell responses to rotavirus share common immunodominant variable gene repertoires. J Immunol, 171(9), 4680-8, 2003.

Weitkamp, JH, Kallewaard, N, Kusuhara, K, Feigelstock, D, Feng, N, Greenberg, HB, Crowe, JE. Generation of recombinant human monoclonal antibodies to rotavirus from single antigen-specific B cells selected with fluorescent virus-like particles. J Immunol Methods, 275(1-2), 223-37, 2003.

Weitkamp, JH, Reinsberg, J, Bartmann, P. Interleukin-8 (IL-8) preferable to IL-6 as a marker for clinical infection. Clin Diagn Lab Immunol, 9(6), 1401, 2002. PMCID:130121

Crowe, JE, Suara, RO, Brock, S, Kallewaard, N, House, F, Weitkamp, JH. Genetic and structural determinants of virus neutralizing antibodies. Immunol Res, 23(2-3), 135-45, 2001.

Weitkamp, JH, Crowe, JE. Blood donor leukocyte reduction filters as a source of human B lymphocytes. Biotechniques, 31(3), 464, 466, 2001.

Weitkamp, JH, St??ber, F, Bartmann, P. Pilot study assessing TNF gene polymorphism as a prognostic marker for disease progression in neonates with sepsis. Infection, 28(2), 92-6, 2000.

Weitkamp, JH, Tang, YW, Haas, DW, Midha, NK, Crowe, JE. Recurrent Achromobacter xylosoxidans bacteremia associated with persistent lymph node infection in a patient with hyper-immunoglobulin M syndrome. Clin Infect Dis, 31(5), 1183-7, 2000.

Weitkamp, JH, Poets, CF, Sievers, R, Musswessels, E, Groneck, P, Thomas, P, Bartmann, P. Candida infection in very low birth-weight infants: outcome and nephrotoxicity of treatment with liposomal amphotericin B (AmBisome). Infection, 26(1), 11-5, 1998.

Weitkamp, JH, P??rez-P??rez, GI, Bode, G, Malfertheiner, P, Blaser, MJ. Identification and characterization of Helicobacter pylori phospholipase C activity. Zentralbl Bakteriol, 280(1-2), 11-27, 1993.


Postdoctoral Position Available
Yes

Postdoctoral Position Details
Motivated candidates with a strong background in immunology, cell biology, or molecular biology are encouraged to apply. We are looking for a dedicated and scientifically curious individual with research experience in flow cytometry, histochemistry, cell culture or signal transduction. Additional training as necessary will be provided.

Updated Date
05/22/2012



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