Vanderbilt University School of Medicine

Prince, Lawrence S , MD, PhD
Adjunct Associate Professor of Pediatrics

Lab Url: N/A

Phone Number: 322-6220

Email Address:lance.prince@vanderbilt.edu

Prince, Lawrence's picture

Office Address   Mailing Address

9425C MRB IV

9435-A MRB IV 37232-0493


Research Keywords
inflammation, branching morphogenesis, cell-matrix interactions, toll-like receptors, vascular development, transgenic reporters, live cell microscopy,Developmental biology,Immunology,Mouse,Pulmonary,Stem cells,Transcription factor,Vascular Biology

Research Description
Disruption of normal lung development causes disease in many children. Our laboratory studies the molecular mechanisms leading to bronchopulmonary dysplasia (BPD), a common complication of preterm birth. Exposure to inflammation during early stages of lung development leads to BPD. We focus much of our work on how inflammatory signals can interfere with normal lung morphogenesis. One project studies how Toll-Like Receptor signaling inhibits FGF-10 expression in the lung mesenchyme. FGF-10 is a critical growth factor for lung development, and is reduced in patients with BPD in addition to mice exposed in utero to inflammation. We are using many different approaches to understand how inflammatory cytokines and specific signaling pathways can regulate FGF-10 expression. By using the Cre-Lox system in transgenic mice, we are deleting components of inflammatory signaling pathways in specific cell types in mouse lungs to examine how they regulate FGF-10 expression and lung branching morphogenesis. We are also developing several novel transgenic mouse reporter models to determine the fate of specific cell types during normal and abnormal lung development. By using live-cell and live-tissue microscopy, we can image cellular movement, membrane dynamics, and matrix interactions in real time. These approaches are giving us new insights into the cellular and molecular mechanisms occurring during lung development.

Publications
Blackwell, TS, Hipps, AN, Yamamoto, Y, Han, W, Barham, WJ, Ostrowski, MC, Yull, FE, Prince, LS. NF-{kappa}B Signaling in Fetal Lung Macrophages Disrupts Airway Morphogenesis. J Immunol, 187(5), 2740-7, 2011.

Benjamin, JT, Carver, BJ, Plosa, EJ, Yamamoto, Y, Miller, JD, Liu, JH, van der Meer, R, Blackwell, TS, Prince, LS. NF-kappaB activation limits airway branching through inhibition of Sp1-mediated fibroblast growth factor-10 expression. J Immunol, 185(8), 4896-903, 2010.

Miller, JD, Benjamin, JT, Kelly, DR, Frank, DB, Prince, LS. Chorioamnionitis stimulates angiogenesis in saccular stage fetal lungs via CC chemokines. Am J Physiol Lung Cell Mol Physiol, 298(5), L637-45, 2010. PMCID:2867403

Benjamin, JT, Gaston, DC, Halloran, BA, Schnapp, LM, Zent, R, Prince, LS. The role of integrin alpha8beta1 in fetal lung morphogenesis and injury. Dev Biol, 335(2), 407-17, 2009.

Wang, H, Ding, T, Brown, N, Yamamoto, Y, Prince, LS, Reese, J, Paria, BC. Zonula occludens-1 (ZO-1) is involved in morula to blastocyst transformation in the mouse. Dev Biol, 318(1), 112-25, 2008. PMCID:2442465

Benjamin, JT, Smith, RJ, Halloran, BA, Day, TJ, Kelly, DR, Prince, LS. FGF-10 is decreased in bronchopulmonary dysplasia and suppressed by Toll-like receptor activation. Am J Physiol Lung Cell Mol Physiol, 292(2), L550-8, 2007.

Dieperink, HI, Blackwell, TS, Prince, LS. Hyperoxia and apoptosis in developing mouse lung mesenchyme. Pediatr Res, 59(2), 185-90, 2006.

Prince, LS, Dieperink, HI, Okoh, VO, Fierro-Perez, GA, Lallone, RL. Toll-like receptor signaling inhibits structural development of the distal fetal mouse lung. Dev Dyn, 233(2), 553-61, 2005.

Carlo, WA, Prince, LS, St John, EB, Ambalavanan, N. Care of very low birth weight infants with respiratory distress syndrome: an evidence-based review. Minerva Pediatr, 56(4), 373-80, 2004.

Prince, LS, Okoh, VO, Moninger, TO, Matalon, S. Lipopolysaccharide increases alveolar type II cell number in fetal mouse lungs through Toll-like receptor 4 and NF-kappaB. Am J Physiol Lung Cell Mol Physiol, 287(5), L999-1006, 2004.

Prince, LS, Karp, PH, Moninger, TO, Welsh, MJ. KGF alters gene expression in human airway epithelia: potential regulation of the inflammatory response. Physiol Genomics, 6(2), 81-9, 2001.

Prince, LS, Launspach, JL, Geller, DS, Lifton, RP, Pratt, JH, Zabner, J, Welsh, MJ. Absence of amiloride-sensitive sodium absorption in the airway of an infant with pseudohypoaldosteronism. J Pediatr, 135(6), 786-9, 1999.

Prince, LS, Peter, K, Hatton, SR, Zaliauskiene, L, Cotlin, LF, Clancy, JP, Marchase, RB, Collawn, JF. Efficient endocytosis of the cystic fibrosis transmembrane conductance regulator requires a tyrosine-based signal. J Biol Chem, 274(6), 3602-9, 1999.

Prince, LS, Welsh, MJ. Effect of subunit composition and Liddle's syndrome mutations on biosynthesis of ENaC. Am J Physiol, 276(6 Pt 1), C1346-51, 1999.

Cheng, C, Prince, LS, Snyder, PM, Welsh, MJ. Assembly of the epithelial Na+ channel evaluated using sucrose gradient sedimentation analysis. J Biol Chem, 273(35), 22693-700, 1998.

Goulet, CC, Volk, KA, Adams, CM, Prince, LS, Stokes, JB, Snyder, PM. Inhibition of the epithelial Na+ channel by interaction of Nedd4 with a PY motif deleted in Liddle's syndrome. J Biol Chem, 273(45), 30012-7, 1998.

Prince, LS, Welsh, MJ. Cell surface expression and biosynthesis of epithelial Na+ channels. Biochem J, 336 ( Pt 3), 705-10, 1998. PMCID:1219923

Snyder, PM, Cheng, C, Prince, LS, Rogers, JC, Welsh, MJ. Electrophysiological and biochemical evidence that DEG/ENaC cation channels are composed of nine subunits. J Biol Chem, 273(2), 681-4, 1998.

Prince, LS, Workman, RB, Marchase, RB. Rapid endocytosis of the cystic fibrosis transmembrane conductance regulator chloride channel. Proc Natl Acad Sci U S A, 91(11), 5192-6, 1994. PMCID:43958

Prince, LS, Tousson, A, Marchase, RB. Cell surface labeling of CFTR in T84 cells. Am J Physiol, 264(2 Pt 1), C491-8, 1993.

Prince, LS, Miller, SK, Pohost, GM, Elgavish, GA. The longitudinal relaxation time (T1) of the intracellular 23Na NMR signal in the isolated perfused rat heart during hypoxia and reoxygenation. Magn Reson Med, 23(2), 376-82, 1992.


Postdoctoral Position Available
Yes

Postdoctoral Position Details
We currently have postdoctoral positions available in our lab. If interested in our research, please send a c.v. and the names of 3 references to Lance.

Updated Date
11/08/2011



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