Vanderbilt University School of Medicine

De Jonge, Niels , PhD
Adjoint Assistant Professor of Molecular Physiology and Biophysics

Lab Url: http://www.mc.vanderbilt.edu/labs/dejongelab/

Phone Number: +49-681-9300-313

Email Address:niels.de.jonge (at) vanderbilt.edu

De Jonge, Niels's picture

Office Address   Mailing Address

INM-Leibniz Institute for new Materials, 66386


Research Keywords
Molecular-level imaging, electron microscopy, biophysics, cell biology, protein function, nanobiotechnology, nanotechnology

Research Specialty
Electron microscopy of cells

Research Description
Molecular-level imaging with new electron microscopy techniques for biomedical research. This research program aims at developing new scanning transmission electron microscopy (STEM) techniques to investigate the molecular interaction underlying cellular function. Electron microscopy presently has its strength in imaging with high spatial resolution. New phenomena may be discovered by improving the capabilities of time-resolved and three-dimensional (3D) imaging under native (liquid) conditions. The program includes three research projects:

Liquid STEM. Research on scanning transmission electron microscopy (STEM) of whole eukaryotic cells in liquid using a flow cell with electron transparent windows. Nanometer resolution was achieved on specific labels. Liquid STEM is also used in correlative light- and electron microscopy, and can be used for in situ experiments in materials science.
See also: http://www.vanderbilt.edu/exploration/stories/liquidstem.html

3D STEM. Research on 3-dimensional aberration corrected STEM of biological samples based on the principles of confocal microscopy. 3D STEM is an alternative for electron tomography with potentially improved capabilities.

Electron microscopy is conducted at the INM-Leibniz Institute in Saarbruecken, Germany, using the JEOL ARM 200 F aberration corrected STEM/TEM.

Publications
Dahmen, T, Baudoin, JP, Lupini, AR, K??bel, C, Slusallek, P, de Jonge, N. Combined Scanning Transmission Electron Microscopy Tilt- and Focal Series. Microsc Microanal, , 1-13, 2014.

Peckys, DB, Dukes, MJ, de Jonge, N. Correlative fluorescence and electron microscopy of quantum dot labeled proteins on whole cells in liquid. Methods Mol Biol, 1117, 527-40, 2014.

Peckys, DB, de Jonge, N. Liquid Scanning Transmission Electron Microscopy: Imaging Protein Complexes in their Native Environment in Whole Eukaryotic Cells. Microsc Microanal, , 1-20, 2014.

Peckys, DB, de Jonge, N. Gold nanoparticle uptake in whole cells in liquid examined by environmental scanning electron microscopy. Microsc Microanal, 20(1), 189-97, 2014.

de Jonge, N. Introduction to Special Issue on Electron Microscopy of Specimens in Liquid. Microsc Microanal, , 1-2, 2014.

Baudoin, JP, Jerome, WG, K??bel, C, de Jonge, N. Whole-Cell Analysis of Low-Density Lipoprotein Uptake by Macrophages Using STEM Tomography. PLoS One, 8(1), e55022, 2013.

Baudoin, JP, Jinschek, JR, Boothroyd, CB, Dunin-Borkowski, RE, de Jonge, N. Chromatic aberration-corrected tilt series transmission electron microscopy of nanoparticles in a whole mount macrophage cell. Microsc Microanal, 19(4), 814-20, 2013.

Kraus, T, de Jonge, N. Dendritic gold nanowire growth observed in liquid with transmission electron microscopy. Langmuir, 29(26), 8427-32, 2013.

Peckys, DB, Baudoin, JP, Eder, M, Werner, U, de Jonge, N. Epidermal growth factor receptor subunit locations determined in hydrated cells with environmental scanning electron microscopy. Sci Rep, 3, 2626, 2013.

Ramachandra, R, Demers, H, de Jonge, N. The influence of the sample thickness on the lateral and axial resolution of aberration-corrected scanning transmission electron microscopy. Microsc Microanal, 19(1), 93-101, 2013.

de Jonge, N. Proceedings of the Conference on In-Situ and Correlative Electron Microscopy (CISCEM), November 6-7, 2012, Saarbrucken, Germany. Advances in Imaging & Electron Physics, 179, 137-202, 2013.

Demers, H, Ramachandra, R, Drouin, D, de Jonge, N. The probe profile and lateral resolution of scanning transmission electron microscopy of thick specimens. Microsc Microanal, 18(3), 582-90, 2012.

Heeres, EC, Oosterkamp, TH, de Jonge, N. Size of the localized electron emission sites on a closed multiwalled carbon nanotube. Phys Rev Lett, 108(3), 036804, 2012.

Ramachandra, R, de Jonge, N. Optimized deconvolution for maximum axial resolution in three-dimensional aberration-corrected scanning transmission electron microscopy. Microsc Microanal, 18(1), 218-28, 2012.

Ring, EA, de Jonge, N. Video-frequency scanning transmission electron microscopy of moving gold nanoparticles in liquid. Micron, 43(11), 1078-84, 2012.

Demers, H, Poirier-Demers, N, Couture, AR, Joly, D, Guilmain, M, de Jonge, N, Drouin, D. Three-dimensional electron microscopy simulation with the CASINO Monte Carlo software. Scanning, 33(3), 135-46, 2011.

Demers, H, Poirier-Demers, N, Couture, ER, Joly, D, Guilman, M, de Jonge, N, Drouin, D. Three-dimensional electron microscopy simulation with the CASINO Monte Carlo software. Scanning, 33, 135-146, 2011.

Dukes, MJ, Ramachandra, R, Baudoin, JP, Gray Jerome, W, de Jonge, N. Three-dimensional locations of gold-labeled proteins in a whole mount eukaryotic cell obtained with 3nm precision using aberration-corrected scanning transmission electron microscopy. J Struct Biol, 174(3), 552-62, 2011.

Heeres, EC, Oosterkamp, TH, de Jonge, N. Making carbon nanotube electron sources of defined lengths and with closed caps. Nanotechnology, 22, 235308-1-8, 2011.

Klein, KL, Anderson, IM, DE Jonge, N. Transmission electron microscopy with a liquid flow cell. J Microsc, 242, 117-123, 2011.

Lupini, AR, de Jonge, N. The three-dimensional point spread function of aberration-corrected scanning transmission electron microscopy. Microsc Microanal, 17(5), 817-26, 2011.

Peckys, DB, Mazur, P, Gould, KL, de Jonge, N. Fully hydrated yeast cells imaged with electron microscopy. Biophys J, 100(10), 2522-9, 2011.

Peckys, DB, de Jonge, N. Visualizing gold nanoparticle uptake in live cells with liquid scanning transmission electron microscopy. Nano Lett, 11(4), 1733-8, 2011.

Ramachandra, R, Demers, H, de Jonge, N. Atomic-resolution scanning transmission electron microscopy through 50-nm-thick silicon nitride membranes. Appl Phys Lett, 98(9), 93109, 2011.

Ramachandra, R, Demers, H, de Jonge, N. Atomic resolution through silicon nitride membranes. Appl. Phys. Lett., 98, 93109-1-3, 2011.

Ring, EA, Peckys, DB, Dukes, MJ, Baudoin, JP, de Jonge, N. Silicon nitride windows for electron microscopy of whole cells. J Microsc, 243(3), 273-83, 2011.

de Jonge, N, Ross, FM. Electron microscopy of specimens in liquid. Nat Nanotechnol, 6(11), 695-704, 2011.

Demers, H, Poirier-Demers, N, Drouin, D, de Jonge, N. Simulating STEM imaging of nanoparticles in micrometers-thick substrates. Microsc Microanal, 16(6), 795-804, 2010.

Dukes, MJ, Peckys, DB, de Jonge, N. Correlative Fluorescence Microscopy and Scanning Transmission Electron Microscopy of Quantum-Dot-Labeled Proteins in Whole Cells in Liquid. ACS Nano, 4, 4110-4116, 2010.

Jarvis, JD, Andrews, HL, Ivanov, B, Stewart, CL, de Jonge, N, Heeres, EC, Kang, WP, Wong, YM, Davidson, JL, Brau, CA. Resonant tunneling and extreme brightness from diamond field emitters and carbon nanotubes. J. Appl. Phys., 108, 94322-1-6, 2010.

Ring, EA, de Jonge, N. Microfluidic System for Transmission Electron Microscopy. Microsc Microanal, 16, 622-629, 2010.

de Jonge, N. Fundamental properties of CNT emitters as an electron source. in: Carbon nanotube and related field emitters: fundamentals and applications, ed. Saito, Y., , , 2010.

de Jonge, N, Bigelow, WC, Veith, GM. Atmospheric pressure scanning transmission electron microscopy. Nano Lett, 10(3), 1028-31, 2010.

de Jonge, N, Poirier-Demers, N, Demers, H, Peckys, DB, Drouin, D. Nanometer-resolution electron microscopy through micrometers-thick water layers. Ultramicroscopy, 110, 1114-1119, 2010.

de Jonge, N, Sougrat, R, Northan, BM, Pennycook, SJ. Three-dimensional scanning transmission electron microscopy of biological specimens. Microsc Microanal, 16(1), 54-63, 2010.

Peckys, DB, Veith, GM, Joy, DC, de Jonge, N. Nanoscale imaging of whole cells using a liquid enclosure and a scanning transmission electron microscope. PLoS One, 4(12), e8214, 2009.

de Jonge, N. Carbon nanotube electron sources for electron microscopes. Advances in Imaging & Electron Physics, 156, 2159-2164, 2009.

de Jonge, N, Peckys, DB, Kremers, GJ, Piston, DW. Electron microscopy of whole cells in liquid with nanometer resolution. Proc Natl Acad Sci U S A, 106, 2159-2164, 2009. PMCID:2650183

Peckys, DB, de Jonge, N, Simpson, ML, McKnight, TE. End-specific strategies of attachment of long double stranded DNA onto gold-coated nanofiber arrays. Nanotechnology, 19, 435301-10, 2008.

Edgcombe, CJ, de Jonge, N. Deduction of work function of carbon nanotube field emitter by use of curved-surface theory. J. Phys. D: Appl. Phys., 40, 4123-4128, 2007.

Heeres, EC, Bakkers, EP, Roest, AL, Kaiser, M, Oosterkamp, TH, de Jonge, N. Electron emission from individual indium arsenide semiconductor nanowires. Nano Lett, 7(2), 536-40, 2007.

Lupini, AR, Rashkeev, SN, Varela, M, Borisevich, AY, Oxley, MP, van Benthem, K, Peng, Y, de Jonge, N, Veith, GM, Chisholm, MF, Pennycook, SJ. Scanning transmission electron microscopy. in: Nanocharacterization, ed. Kirkland, E.J. & Hutchison, , 28-65, 2007.

de Jonge, N, Sougrat, R, Peckys, DB, Lupini, AR, Pennycook, SJ. 3-dimensional aberration corrected scanning transmission electron microscopy for biology. in: Nanotechnology in Biology and Medicine, ed. Vo-Dinh, T, , 13.1-27, 2007.

Doytcheva, M, Kaiser, M, de Jonge, N. In-situ transmission electron microscopy investigation of the structural changes in carbon nanotubes during electron emission at high currents. Nanotechnology, 17, 3226-3233, 2006.

Edgcombe, CJ, de Jonge, N. Preparation of a carbon nanotube field emitter and deduction of its properties from curvature the Fowler-Nordheim plot. J. Phys. Conf. S., 26, 315-318, 2006.

Edgcombe, CJ, de Jonge, N. Properties of a field emitter deduced from curvature of its Fowler-Nordheim plot. J. Vac. Sci. Technol B, 24, 869-873, 2006.

Kaiser, M, Doytcheva, M, Verheijen, M, de Jonge, N. In situ transmission electron microscopy observations of individually selected freestanding carbon nanotubes during field emission. Ultramicroscopy, 106(10), 902-8, 2006.

Milne, WI, Teo, KBK, Mann, M, Bu, IYY, Amaratunga, GAJ, de Jonge, N, Allioux, M, Oostveen, JT, Legagneux, P, Minoux, E, Gangloff, L, Hudanski, L, Schnell, JP, Dieumegard, LD, Peauger, F, Wells, T, El-Gomati, M. Carbon nanotubes as electron sources. Phys. Stat. Sol., 203, 1058-1063, 2006.

de Jonge, N, Allioux, M, Oostveen, JT, Teo, KB, Milne, WI. Optical performance of carbon-nanotube electron sources. Phys Rev Lett, 94(18), 186807, 2005.

de Jonge, N, Allioux, M, Oostveen, JT, Teo, KBK, Milne, WI. Low noise and stable emission from carbon nanotube electron sources. Appl. Phys. Lett., 87, 133118-1-3, 2005.

de Jonge, N, Doytcheva, M, Allioux, M, Kaiser, M, Mentink, SAM, Teo, KBK, Lacerda, RG, Milne, WI. Cap closing of thin carbon nanotubes. Adv. Mater., 17, 451-455, 2005.

De Jonge, N Allioux, M, Doytcheva, M, Kaiser, M, Teo, KBK, Lacerda, RG, Milne, WI. Characterization of the field emission properties of individual thin carbon nanotubes. Appl. Phys. Lett., 85, 1607-1609, 2004.

Doytcheva, M, Kaiser, M, Verheijen, MA, Reyes-Reyes, M, Terrones, M, de Jonge, N. Electron emission from individual nitrogen-doped multi-walled carbon nanotubes. Chem. Phys. Lett., 396, 126-130, 2004.

de Jonge, N. The brightness of carbon nanotube electron emitters. J. Appl. Phys., 95, 673-681, 2004.

de Jonge, N, Allioux, M, Doytcheva, M, Kaiser, M, Teo, KBK, Lacerda, RG, Milne, WI. Field emission from individual thin carbon nanotubes. AIP Conference Proceedings, N723, 485-489, 2004.

de Jonge, N, Bonard, JM. Carbon nanotube electron sources and applications. Philos Transact A Math Phys Eng Sci, 362(1823), 2239-66, 2004.

de Jonge, N, Lamy, Y, Kaiser, M. Controlled mounting of individual multi-walled carbon nanotubes on support tips. Nano Lett., 3, 1621-1624, 2003.

de Jonge, N, van Druten, NJ. Field emission from individual multiwalled carbon nanotubes prepared in an electron microscope. Ultramicroscopy, 95(1-4), 85-91, 2003.

de Jonge, N, Lamy, Y, Schoots, K, Oosterkamp, TH. High brightness electron beam from a multi-walled carbon nanotube. Nature, 420(6914), 393-5, 2002.

Rau, HK, DeJonge, N, Haehnel, W. Combinatorial Synthesis of Four-Helix Bundle Hemoproteins for Tuning of Cofactor Properties. Angew Chem Int Ed Engl, 39(1), 250-253, 2000.

de Jonge, N, Rau, HK, Haehnel, W. Light induced electron transfer in synthetic metalloproteins. Z. Phys. Chem., 213, 175-180, 1999.

Rau, HK, DeJonge, N, Haehnel, W. Modular synthesis of de novo-designed metalloproteins for light-induced electron transfer. Proc Natl Acad Sci U S A, 95(20), 11526-31, 1998. PMCID:21674

van den Brink, HB, et al.. Neutral-pion electroproduction on the proton near threshold. Phys. Rev. Lett., 74, 3561-3564, 1995.

Bobeldijk, I, et al.. High-momentum protons in /sup 208/Pb. Phys. Rev. Lett., 73, 2684-2687, 1994.


Postdoctoral Position Available
No

Postdoctoral Position Details
N/A

Updated Date
09/13/2013



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