Vanderbilt University School of Medicine

Sebzda, Eric , PhD
Assistant Professor of Pathology, Microbiology and Immunology

Lab Url: https://my.vanderbilt.edu/ericsebzda/

Phone Number: 615 343-6050

Email Address:eric.sebzda@vanderbilt.edu

Sebzda, Eric's picture

Office Address   Mailing Address

A5221 MCN

A5211 MCN 37232-2363


Research Keywords
lymphocytes, T cells, autoimmunity, cell migration, chemokine receptors, chemokines, ,Cell cycle,Gene regulation,Immunology,Infectious disease,Knockout,Microbiology,Molecular medicine,Mouse,Proteomics,Signal transduction,Transcription factor

Research Specialty
We investigate the molecular mechanisms that underpin immunological diseases including inefficient responses to pathogens, unwarranted inflammation, and autoimmunity.

Research Description
A unique aspect of the immune system is its ability to transport cells great distances in a short period of time. These trafficking mechanisms dictate the efficiency of an immune response, both in terms of pathogen clearance and susceptibility to autoimmune diseases. The Sebzda laboratory studies B and T cell trafficking during immune responses. Specially, we are interested in determining how cell activation releases these lymphocytes from a homeostatic migration pattern and targets these cells to sites of inflammation. We are currently using a series of genetically modified animal models to analyze early regulatory events in cellular trafficking. The ultimate goal of this research is to identify potential therapeutic targets that enhance/inhibit lymphocyte trafficking to specific tissues and thus aid in pathogen clearance or limit autoimmune reactions, respectively.

Publications
Bertozzi, CC, Schmaier, AA, Mericko, P, Hess, PR, Zou, Z, Chen, M, Chen, CY, Xu, B, Lu, MM, Zhou, D, Sebzda, E, Santore, MT, Merianos, DJ, Stadtfeld, M, Flake, AW, Graf, T, Skoda, R, Maltzman, JS, Koretzky, GA, Kahn, ML. Platelets regulate lymphatic vascular development through CLEC-2-SLP-76 signaling. Blood, 116(4), 661-70, 2010.

Hoek, KL, Gordy, LE, Collins, PL, Parekh, VV, Aune, TM, Joyce, S, Thomas, JW, Van Kaer, L, Sebzda, E. Follicular B Cell Trafficking within the Spleen Actively Restricts Humoral Immune Responses. Immunity, , , 2010.

Sebzda, E, Zou, Z, Lee, JS, Wang, T, Kahn, ML. Transcription factor KLF2 regulates the migration of naive T cells by restricting chemokine receptor expression patterns. Nat Immunol, 9(3), 292-300, 2008.

Abtahian, F, Bezman, N, Clemens, R, Sebzda, E, Cheng, L, Shattil, SJ, Kahn, ML, Koretzky, GA. Evidence for the requirement of ITAM domains but not SLP-76/Gads interaction for integrin signaling in hematopoietic cells. Mol Cell Biol, 26(18), 6936-49, 2006. PMCID:1592869

Chen, H, Zou, Z, Sarratt, KL, Zhou, D, Zhang, M, Sebzda, E, Hammer, DA, Kahn, ML. In vivo beta1 integrin function requires phosphorylation-independent regulation by cytoplasmic tyrosines. Genes Dev, 20(8), 927-32, 2006. PMCID:1472300

Lee, JS, Yu, Q, Shin, JT, Sebzda, E, Bertozzi, C, Chen, M, Mericko, P, Stadtfeld, M, Zhou, D, Cheng, L, Graf, T, MacRae, CA, Lepore, JJ, Lo, CW, Kahn, ML. Klf2 is an essential regulator of vascular hemodynamic forces in vivo. Dev Cell, 11(6), 845-57, 2006.

Sebzda, E, Hibbard, C, Sweeney, S, Abtahian, F, Bezman, N, Clemens, G, Maltzman, JS, Cheng, L, Liu, F, Turner, M, Tybulewicz, V, Koretzky, GA, Kahn, ML. Syk and Slp-76 mutant mice reveal a cell-autonomous hematopoietic cell contribution to vascular development. Dev Cell, 11(3), 349-61, 2006.

Abtahian, F, Guerriero, A, Sebzda, E, Lu, MM, Zhou, R, Mocsai, A, Myers, EE, Huang, B, Jackson, DG, Ferrari, VA, Tybulewicz, V, Lowell, CA, Lepore, JJ, Koretzky, GA, Kahn, ML. Regulation of blood and lymphatic vascular separation by signaling proteins SLP-76 and Syk. Science, 299(5604), 247-51, 2003.

Sebzda, E, Bracke, M, Tugal, T, Hogg, N, Cantrell, DA. Rap1A positively regulates T cells via integrin activation rather than inhibiting lymphocyte signaling. Nat Immunol, 3(3), 251-8, 2002.

Ohteki, T, Hessel, A, Bachmann, MF, Zakarian, A, Sebzda, E, Tsao, MS, McKall-Faienza, K, Odermatt, B, Ohashi, PS. Identification of a cross-reactive self ligand in virus-mediated autoimmunity. Eur J Immunol, 29(9), 2886-96, 1999.

Sebzda, E, Mariathasan, S, Ohteki, T, Jones, R, Bachmann, MF, Ohashi, PS. Selection of the T cell repertoire. Annu Rev Immunol, 17, 829-74, 1999.

Sebzda, E, Choi, M, Fung-Leung, WP, Mak, TW, Ohashi, PS. Peptide-induced positive selection of TCR transgenic thymocytes in a coreceptor-independent manner. Immunity, 6(5), 643-53, 1997.

Bachmann, MF, Sebzda, E, Kundig, TM, Shahinian, A, Speiser, DE, Mak, TW, Ohashi, PS. T cell responses are governed by avidity and co-stimulatory thresholds. Eur J Immunol, 26(9), 2017-22, 1996.

Kundig, TM, Shahinian, A, Kawai, K, Mittr??cker, HW, Sebzda, E, Bachmann, MF, Mak, TW, Ohashi, PS. Duration of TCR stimulation determines costimulatory requirement of T cells. Immunity, 5(1), 41-52, 1996.

Sebzda, E, Kundig, TM, Thomson, CT, Aoki, K, Mak, SY, Mayer, JP, Zamborelli, T, Nathenson, SG, Ohashi, PS. Mature T cell reactivity altered by peptide agonist that induces positive selection. J Exp Med, 183(3), 1093-104, 1996. PMCID:2192317

Speiser, DE, Sebzda, E, Ohteki, T, Bachmann, MF, Pfeffer, K, Mak, TW, Ohashi, PS. Tumor necrosis factor receptor p55 mediates deletion of peripheral cytotoxic T lymphocytes in vivo. Eur J Immunol, 26(12), 3055-60, 1996.

Sebzda, E, Wallace, VA, Mayer, J, Yeung, RS, Mak, TW, Ohashi, PS. Positive and negative thymocyte selection induced by different concentrations of a single peptide. Science, 263(5153), 1615-8, 1994.


Postdoctoral Position Available
Yes

Postdoctoral Position Details
Two postdoctoral positions are currently available in the Department of Microbiology and Immunology, at Vanderbilt University to study a unique aspect of chemokine receptor regulation in lymphocytes (Nature Immunology (2008) 9, 292-300). Studies are focused on addressing how the transcription factor, Kruppel-like factor 2 (Klf2), represses inflammatory chemokine receptor transcription while maintaining expression of homeostatic migratory receptors. As well, the laboratory is studying how aberrant naive T cell trafficking affects the immune system, both in terms of pathogen clearance and susceptibility to autoimmune disorders.

Applicants should possess a PhD or MD/PhD in Microbiology, Genetics, Immunology or a related field. Candidates applying for the Molecular Immunology position should be proficient in molecular biology techniques including protein purification, Westerns, gel-shift assays, transcription reporter assays, and RT-PCR. Candidates applying for the Cellular Immunology position should be adept at cell culture techniques, flow cytometry, and analysis of primary mouse lymphocytes including proliferation, cytokine, and cytolytic assays. Experience working with transgenic and knockout mice is an asset for both positions.

The successful candidates will gain experience in a number of cutting edge molecular and cellular biological techniques. Importantly, both projects have great potential to become independent research projects that would further ambitious, motivated postdoctoral careers.

Please send a curriculum vita, names of three references, and a letter describing research accomplishments and career objectives to:

Dr. Eric Sebzda
Vanderbilt University
Department of Microbiology and Immunology
1161 21st Ave. S
MCN A5211
Nashville, TN 37232

Updated Date
02/20/2013



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