Vanderbilt University School of Medicine

Chen, Wenbiao , Phd
Associate Professor of Molecular Physiology & Biophysics

Lab Url: N/A

Phone Number: 615-936-8126


Chen, Wenbiao's picture

Office Address   Mailing Address

711 Light Hall

8425A MRB IV 0615

Research Keywords
Zebrafish,,Developmental biology,Diabetes,Endocrinology,Genetics,Genomics,Mutation,Physiology,Signal transduction

Research Specialty
Genetic basis of susceptibility to type 2 diabetes.

Research Description
Pancreatic alpha and beta cells secrete glucagon and insulin respectively to maintain glucose homeostasis. In conditions that impair the signaling of these hormones, alpha and beta cells compensate by increasing their number and augmenting their secretion. Defects in the compensatory responses lead to defects in glucose homeostasis. The molecular mechanisms underlying the compensatory responses remain incompletely understood. The Chen laboratory found that compensatory responses of pancreatic alpha cells and beta cells is conserved in zebrafish, a vertebrate model amenable for live imaging as well as large-scale genetic and chemical screens. We combine genetic, pharmacological, and imaging techniques to identify genes essential for the compensatory responses and to define their roles in diabetes.

Gong, Y, Ma, Y, Sinyuk, M, Loganathan, S, Thompson, RC, Sarkaria, JN, Chen, W, Lathia, JD, Mobley, BC, Clark, SW, Wang, J. Insulin-mediated signaling promotes proliferation and survival of glioblastoma through Akt activation. Neuro Oncol, , , 2015.

Huang, J, Zhong, Z, Wang, M, Chen, X, Tan, Y, Zhang, S, He, W, He, X, Huang, G, Lu, H, Wu, P, Che, Y, Yan, YL, Postlethwait, JH, Chen, W, Wang, H. Circadian modulation of dopamine levels and dopaminergic neuron development contributes to attention deficiency and hyperactive behavior. J Neurosci, 35(6), 2572-87, 2015.

Ju, B, Chen, W, Orr, BA, Spitsbergen, JM, Jia, S, Eden, CJ, Henson, HE, Taylor, MR. Oncogenic KRAS promotes malignant brain tumors in zebrafish. Mol Cancer, 14(1), 18, 2015.

Li, M, Dean, ED, Zhao, L, Nicholson, WE, Powers, AC, Chen, W. Glucagon receptor inactivation leads to ??-cell hyperplasia in zebrafish. J Endocrinol, 227(2), 93-103, 2015.

Li, M, Page-McCaw, P, Chen, W. FGF1 Mediates Overnutrition-Induced Compensatory ??-Cell Differentiation. Diabetes, , , 2015.

Maddison, LA, Joest, KE, Kammeyer, RM, Chen, W. Skeletal muscle insulin resistance in zebrafish induces alterations in ??-cell number and glucose tolerance in an age and diet dependent manner. Am J Physiol Endocrinol Metab, , ajpendo.00441.2014, 2015.

Varshney, GK, Pei, W, LaFave, MC, Idol, J, Xu, L, Gallardo, V, Carrington, B, Bishop, K, Jones, M, Li, M, Harper, U, Huang, SC, Prakash, A, Chen, W, Sood, R, Ledin, J, Burgess, SM. High-throughput gene targeting and phenotyping in zebrafish using CRISPR/Cas9. Genome Res, 25(7), 1030-42, 2015.

Yin, L, Jao, LE, Chen, W. Generation of Targeted Mutations in Zebrafish Using the CRISPR/Cas System. Methods Mol Biol, 1332, 205-17, 2015.

Yin, L, Maddison, LA, Li, M, Kara, N, LaFave, MC, Varshney, GK, Burgess, SM, Patton, JG, Chen, W. Multiplex Conditional Mutagenesis Using Transgenic Expression of Cas9 and sgRNAs. Genetics, 200(2), 431-41, 2015.

Zou, P, Wu, SY, Koteiche, HA, Mishra, S, Levic, DS, Knapik, E, Chen, W, Mchaourab, HS. A conserved role of ??A-crystallin in the development of the zebrafish embryonic lens. Exp Eye Res, 138, 104-113, 2015.

Dirian, L, Galant, S, Coolen, M, Chen, W, Bedu, S, Houart, C, Bally-Cuif, L, Foucher, I. Spatial Regionalization and Heterochrony in the Formation of Adult Pallial Neural Stem Cells. Dev Cell, , , 2014.

Ju, B, Chen, W, Spitsbergen, JM, Lu, J, Vogel, P, Peters, JL, Wang, YD, Orr, BA, Wu, J, Henson, HE, Jia, S, Parupalli, C, Taylor, MR. Activation of Sonic hedgehog signaling in neural progenitor cells promotes glioma development in the zebrafish optic pathway. Oncogenesis, 3, e96, 2014.

Li, M, Maddison, LA, Page-McCaw, P, Chen, W. Overnutrition induces ??-cell differentiation through prolonged activation of ?? cells in zebrafish larvae. Am J Physiol Endocrinol Metab, , , 2014.

Maddison, LA, Li, M, Chen, W. Conditional gene-trap mutagenesis in zebrafish. Methods Mol Biol, 1101, 393-411, 2014.

Jao, LE, Wente, SR, Chen, W. Efficient multiplex biallelic zebrafish genome editing using a CRISPR nuclease system. Proc Natl Acad Sci U S A, , , 2013.

Li, M, Maddison, LA, Crees, Z, Chen, W. Targeted overexpression of CKI-insensitive cyclin-dependent kinase 4 increases functional ??-cell number through enhanced self-replication in zebrafish. Zebrafish, 10(2), 170-6, 2013.

Maddison, LA, Chen, W. Nutrient excess stimulates ??-cell neogenesis in zebrafish. Diabetes, 61(10), 2517-24, 2012.

Ni, TT, Lu, J, Zhu, M, Maddison, LA, Boyd, KL, Huskey, L, Ju, B, Hesselson, D, Zhong, TP, Page-McCaw, PS, Stainier, DY, Chen, W. Conditional control of gene function by an invertible gene trap in zebrafish. Proc Natl Acad Sci U S A, 109(38), 15389-94, 2012.

Lu, J, Maddison, LA, Chen, W. PhiC31 integrase induces efficient site-specific excision in zebrafish. Transgenic Res, 20(1), 183-9, 2011.

Maddison, LA, Lu, J, Chen, W. Generating conditional mutations in zebrafish using gene-trap mutagenesis. Methods Cell Biol, 104, 1-22, 2011.

Zhang, C, Song, Y, Thompson, DA, Madonna, MA, Millhauser, GL, Toro, S, Varga, Z, Westerfield, M, Gamse, J, Chen, W, Cone, RD. Pineal-specific agouti protein regulates teleost background adaptation. Proc Natl Acad Sci U S A, 107(47), 20164-71, 2010.

Boniface, EJ, Lu, J, Victoroff, T, Zhu, M, Chen, W. FlEx-based transgenic reporter lines for visualization of Cre and Flp activity in live zebrafish. Genesis, 47(7), 484-91, 2009.

Ju, B, Spitsbergen, J, Eden, CJ, Taylor, MR, Chen, W. Co-activation of hedgehog and AKT pathways promote tumorigenesis in zebrafish. Mol Cancer, 8, 40, 2009.

Maddison, LA, Lu, J, Victoroff, T, Scott, E, Baier, H, Chen, W. A gain-of-function screen in zebrafish identifies a guanylate cyclase with a role in neuronal degeneration. Mol Genet Genomics, 281(5), 551-63, 2009.

Jao, LE, Maddison, L, Chen, W, Burgess, SM. Using retroviruses as a mutagenesis tool to explore the zebrafish genome. Brief Funct Genomic Proteomic, 7(6), 427-43, 2008.

Wan, L, Almers, W, Chen, W. Two ribeye genes in teleosts: the role of Ribeye in ribbon formation and bipolar cell development. J Neurosci, 25(4), 941-9, 2005.

Chen, W, Casey Corliss, D. Three modules of zebrafish Mind bomb work cooperatively to promote Delta ubiquitination and endocytosis. Dev Biol, 267(2), 361-73, 2004.

Burgess, S, Reim, G, Chen, W, Hopkins, N, Brand, M. The zebrafish spiel-ohne-grenzen (spg) gene encodes the POU domain protein Pou2 related to mammalian Oct4 and is essential for formation of the midbrain and hindbrain, and for pre-gastrula morphogenesis. Development, 129(4), 905-16, 2002.

Chen, W, Burgess, S, Golling, G, Amsterdam, A, Hopkins, N. High-throughput selection of retrovirus producer cell lines leads to markedly improved efficiency of germ line-transmissible insertions in zebra fish. J Virol, 76(5), 2192-8, 2002.

Golling, G, Amsterdam, A, Sun, Z, Antonelli, M, Maldonado, E, Chen, W, Burgess, S, Haldi, M, Artzt, K, Farrington, S, Lin, SY, Nissen, RM, Hopkins, N. Insertional mutagenesis in zebrafish rapidly identifies genes essential for early vertebrate development. Nat Genet, 31(2), 135-40, 2002.

Chen, W, Burgess, S, Hopkins, N. Analysis of the zebrafish smoothened mutant reveals conserved and divergent functions of hedgehog activity. Development, 128(12), 2385-96, 2001.

Amsterdam, A, Burgess, S, Golling, G, Chen, W, Sun, Z, Townsend, K, Farrington, S, Haldi, M, Hopkins, N. A large-scale insertional mutagenesis screen in zebrafish. Genes Dev, 13(20), 2713-24, 1999.

Chen, W, Kelly, MA, Opitz-Araya, X, Thomas, RE, Low, MJ, Cone, RD. Exocrine gland dysfunction in MC5-R-deficient mice: evidence for coordinated regulation of exocrine gland function by melanocortin peptides. Cell, 91(6), 789-98, 1997.

Cone, RD, Lu, D, Koppula, S, Vage, DI, Klungland, H, Boston, B, Chen, W, Orth, DN, Pouton, C, Kesterson, RA. The melanocortin receptors: agonists, antagonists, and the hormonal control of pigmentation. Recent Prog Horm Res, 51, 287-317; discussion 318, 1996.

Hou, X, Li, JJ, Chen, W, Li, SA. Estrogen-induced proto-oncogene and suppressor gene expression in the hamster kidney: significance for estrogen carcinogenesis. Cancer Res, 56(11), 2616-20, 1996.

Chen, W, Shields, TS, Stork, PJ, Cone, RD. A colorimetric assay for measuring activation of Gs- and Gq-coupled signaling pathways. Anal Biochem, 226(2), 349-54, 1995.

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