Vanderbilt University School of Medicine

Young, Jamey D. , Ph.D.
Associate Professor of Chemical and Biomolecular Engineering
Assistant Professor of Molecular Physiology & Biophysics

Lab Url:

Phone Number: 615-343-4253


Young, Jamey's picture

Office Address   Mailing Address

212B Olin Hall

VU Station B #351604 2400 Highland Ave; 107 Olin Hall 37235-1304

Research Specialty
Metabolic engineering and systems biology

Research Description
The central theme of our research is the application of engineering, biochemistry, and molecular biology to quantitatively analyze and redirect cellular metabolism. In particular, we use an approach called metabolic flux analysis (MFA) to quantify the rates, or "fluxes", of intracellular metabolic pathways in a variety of cell models of relevance to medicine and biotechnology. In addition to tool-oriented research aimed at Enhancing MFA Methodologies, we are actively pursuing research in four specific application areas: Diabetes & Obesity, Cancer Metabolism, Cell Culture Engineering, and Photosynthesis Research. Although diverse in their biological scope, all projects rely on our lab's core expertise to integrate numerous metabolic measurements into a quantitative map of cell metabolism using rigorous mathematical models, with the ultimate goal of deciphering how metabolic networks are natively regulated and how they can be further engineered to achieve a desired outcome.

Buescher, JM, Antoniewicz, MR, Boros, LG, Burgess, SC, Brunengraber, H, Clish, CB, DeBerardinis, RJ, Feron, O, Frezza, C, Ghesquiere, B, Gottlieb, E, Hiller, K, Jones, RG, Kamphorst, JJ, Kibbey, RG, Kimmelman, AC, Locasale, JW, Lunt, SY, Maddocks, OD, Malloy, C, Metallo, CM, Meuillet, EJ, Munger, J, N??h, K, Rabinowitz, JD, Ralser, M, Sauer, U, Stephanopoulos, G, St-Pierre, J, Tennant, DA, Wittmann, C, Vander Heiden, MG, Vazquez, A, Vousden, K, Young, JD, Zamboni, N, Fendt, SM. A roadmap for interpreting (13)C metabolite labeling patterns from cells. Curr Opin Biotechnol, 34, 189-201, 2015.

Adebiyi, AO, Jazmin, LJ, Young, JD. (13)C flux analysis of cyanobacterial metabolism. Photosynth Res, , , 2014.

Egnatchik, RA, Leamy, AK, Jacobson, DA, Shiota, M, Young, JD. ER calcium release promotes mitochondrial dysfunction and hepatic cell lipotoxicity in response to palmitate overload. Mol Metab, 3(5), 544-53, 2014.

Egnatchik, RA, Leamy, AK, Noguchi, Y, Shiota, M, Young, JD. Palmitate-induced activation of mitochondrial metabolism promotes oxidative stress and apoptosis in H4IIEC3 rat hepatocytes. Metabolism, 63(2), 283-95, 2014.

Jazmin, LJ, O''Grady, JP, Ma, F, Allen, DK, Morgan, JA, Young, JD. Isotopically nonstationary MFA (INST-MFA) of autotrophic metabolism. Methods Mol Biol, 1090, 181-210, 2014.

Leamy, AK, Egnatchik, RA, Shiota, M, Ivanova, PT, Myers, DS, Brown, HA, Young, JD. Enhanced synthesis of saturated phospholipids is associated with ER stress and lipotoxicity in palmitate treated hepatic cells. J Lipid Res, 55(7), 1478-1488, 2014.

Ma, F, Jazmin, LJ, Young, JD, Allen, DK. Isotopically nonstationary 13C flux analysis of changes in Arabidopsis thaliana leaf metabolism due to high light acclimation. Proc Natl Acad Sci U S A, 111(47), 16967-72, 2014.

Templeton, N, Lewis, A, Dorai, H, Qian, EA, Campbell, MP, Smith, KD, Lang, SE, Betenbaugh, MJ, Young, JD. The impact of anti-apoptotic gene Bcl-2??? expression on CHO central metabolism. Metab Eng, 25, 92-102, 2014.

Wall, ML, Pound, LD, Trenary, I, O''Brien, RM, Young, JD. Novel Stable Isotope Analyses Demonstrate Significant Rates of Glucose Cycling In Mouse Pancreatic Islets. Diabetes, , , 2014.

Young, JD. (13)C metabolic flux analysis of recombinant expression hosts. Curr Opin Biotechnol, 30, 238-45, 2014.

Young, JD. INCA: a computational platform for isotopically non-stationary metabolic flux analysis. Bioinformatics, 30(9), 1333-5, 2014.

Young, JD, Allen, DK, Morgan, JA. Isotopomer measurement techniques in metabolic flux analysis II: mass spectrometry. Methods Mol Biol, 1083, 85-108, 2014.

Duckwall, CS, Murphy, TA, Young, JD. Mapping cancer cell metabolism with(13)C flux analysis: Recent progress and future challenges. J Carcinog, 12, 13, 2013.

Jazmin, LJ, Young, JD. Isotopically nonstationary 13C metabolic flux analysis. Methods Mol Biol, 985, 367-90, 2013.

Leamy, AK, Egnatchik, RA, Young, JD. Molecular mechanisms and the role of saturated fatty acids in the progression of non-alcoholic fatty liver disease. Prog Lipid Res, 52(1), 165-74, 2013.

Murphy, TA, Young, JD. ETA: robust software for determination of cell specific rates from extracellular time courses. Biotechnol Bioeng, 110(6), 1748-58, 2013.

Templeton, N, Dean, J, Reddy, P, Young, JD. Peak antibody production is associated with increased oxidative metabolism in an industrially relevant fed-batch CHO cell culture. Biotechnol Bioeng, 110(7), 2013-24, 2013.

Young, JD. Metabolic flux rewiring in mammalian cell cultures. Curr Opin Biotechnol, 24(6), 1108-15, 2013.

Murphy, TA, Dang, CV, Young, J. Isotopically nonstationary (13)C flux analysis of Myc-induced metabolic reprogramming in B-cells. Metab Eng, , , 2012.

Strand, DW, Jiang, M, Murphy, TA, Yi, Y, Konvinse, KC, Franco, OE, Wang, Y, Young, JD, Hayward, SW. PPARI? isoforms differentially regulate metabolic networks to mediate mouse prostatic epithelial differentiation. Cell Death Dis, 3, e361, 2012.

Noguchi, Y, Young, JD, Aleman, JO, Hansen, ME, Kelleher, JK, Stephanopoulos, G. Tracking cellular metabolomics in lipoapoptosis- and steatosis-developing liver cells. Mol Biosyst, 7(5), 1409-19, 2011.

Srour, O, Young, JD, Eldar, YC. Fluxomers: a new approach for 13C metabolic flux analysis. BMC Syst Biol, 5, 129, 2011.

Young, JD, Shastri, AA, Stephanopoulos, G, Morgan, JA. Mapping photoautotrophic metabolism with isotopically nonstationary (13)C flux analysis. Metab Eng, 13(6), 656-65, 2011.

Noguchi, Y, Nishikata, N, Shikata, N, Kimura, Y, Aleman, JO, Young, JD, Koyama, N, Kelleher, JK, Takahashi, M, Stephanopoulos, G. Ketogenic essential amino acids modulate lipid synthetic pathways and prevent hepatic steatosis in mice. PLoS One, 5(8), e12057, 2010. PMCID:2919399

Reed, JL, Senger, RS, Antoniewicz, MR, Young, JD. Computational approaches in metabolic engineering. J Biomed Biotechnol, 2010, 207414, 2010. PMCID:3092504

Wanna, G, Young, JA, Swanson, E, Haynes, D. Spontaneous otogenic posterior fossa pneumocephalus. Otolaryngol Head Neck Surg, 143(4), 593-4, 2010.

Noguchi, Y, Young, JD, Aleman, JO, Hansen, ME, Kelleher, JK, Stephanopoulos, G. Effect of anaplerotic fluxes and amino acid availability on hepatic lipoapoptosis. J Biol Chem, 284(48), 33425-36, 2009. PMCID:2785187

Young, JD, Henne, KL, Morgan, JA, Konopka, AE, Ramkrishna, D. Integrating cybernetic modeling with pathway analysis provides a dynamic, systems-level description of metabolic control. Biotechnol Bioeng, 100(3), 542-59, 2008. PMCID:2785187

Young, JD, Walther, JL, Antoniewicz, MR, Yoo, H, Stephanopoulos, G. An elementary metabolite unit (EMU) based method of isotopically nonstationary flux analysis. Biotechnol Bioeng, 99(3), 686-99, 2008. PMCID:2785187

Young, JD, Ramkrishna, D. On the matching and proportional laws of cybernetic models. Biotechnol Prog, 23(1), 83-99, 2007. PMCID:2785187

J. D. Young, K. L. Henne, J. A. Morgan, A. E. Konopka and D. Ramkrishna. . Cybernetic modeling of metabolism: towards a framework for rational design of recombinant organisms.. Chemical Engineering Science , 59, 5041-9, 2004.

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