Vanderbilt University School of Medicine

Galli, Aurelio , Ph.D.
Professor of Molecular Physiology and Biophysics
Professor of Psychiatry

Lab Url: http://www.mc.vanderbilt.edu/reporter/?ID=3788

Phone Number: (615) 936-3891

Email Address:aurelio.galli@vanderbilt.edu

Galli, Aurelio's picture

Office Address   Mailing Address

7124 MRB III

465 21st Ave South, 7124 MRB III 37013


Research Keywords
Neurotransmitter,Transport,Amphetamine,Cocaine,Insulin,Obesity, Diabetes, Neuroscience,C. elegans

Research Specialty
Trafficking and Biophysics of Neurotransmitter Transporters with emphasis on metabolic regulation of dopamine signaling

Research Description
Food intake is regulated by several neurotransmitters, including dopamine, GABA, norepinephrine, and serotonin, as well as by peptides and amino acids. Dopamine is of special interest because it seems to regulate food intake by modulating the ?reward? circuitry of the brain.
The involvement of dopamine in pathological eating and obesity is not yet well understood, but it is an area of intense interest in our laboratory. Using mouse and rat models, our laboratory investigates how food intake regulates neurotransmitter transporter function. Neurotransmitter transporters are integral membrane proteins responsible for clearing neurotransmitters (e.g., dopamine) from the synaptic cleft, to control the magnitude and duration of synaptic signaling. They include the dopamine (DAT) and norepinephrine (NET) transporters. Substances of abuse such as cocaine and amphetamine, or drugs with clinical relevance such as Ritalin (used for treatment of ADHD), act upon these transporters to alter the concentration of dopamine in the synaptic cleft. The focus of our laboratory is to try to understand the mechanisms of action of these drugs with the intent to improve the pharmacological treatment of specific neurological disorders and substance abuse?and now perhaps also obesity and type 2 diabetes.

Publications
Bowton, E, Saunders, C, Erreger, K, Sakrikar, D, Matthies, HJ, Sen, N, Jessen, T, Colbran, RJ, Caron, MG, Javitch, JA, Blakely, RD, Galli, A. Dysregulation of dopamine transporters via dopamine D2 autoreceptors triggers anomalous dopamine efflux associated with attention-deficit hyperactivity disorder. J Neurosci, 30(17), 6048-57, 2010. PMCID:2882427

Matthies, HJ, Moore, JL, Saunders, C, Matthies, DS, Lapierre, LA, Goldenring, JR, Blakely, RD, Galli, A. Rab11 supports amphetamine-stimulated norepinephrine transporter trafficking. J Neurosci, 30(23), 7863-77, 2010. PMCID:2882427

Robertson, SD, Matthies, HJ, Owens, WA, Sathananthan, V, Christianson, NS, Kennedy, JP, Lindsley, CW, Daws, LC, Galli, A. Insulin reveals Akt signaling as a novel regulator of norepinephrine transporter trafficking and norepinephrine homeostasis. J Neurosci, 30(34), 11305-16, 2010.

Siuta, MA, Robertson, SD, Kocalis, H, Saunders, C, Gresch, PJ, Khatri, V, Shiota, C, Kennedy, JP, Lindsley, CW, Daws, LC, Polley, DB, Veenstra-Vanderweele, J, Stanwood, GD, Magnuson, MA, Niswender, KD, Galli, A. Dysregulation of the norepinephrine transporter sustains cortical hypodopaminergia and schizophrenia-like behaviors in neuronal rictor null mice. PLoS Biol, 8(6), e1000393, 2010. PMCID:2882427

Matthies, HJ, Han, Q, Shields, A, Wright, J, Moore, JL, Winder, DG, Galli, A, Blakely, RD. Subcellular localization of the antidepressant-sensitive norepinephrine transporter. BMC Neurosci, 10, 65, 2009. PMCID:2716352

Robertson, SD, Matthies, HJ, Galli, A. A closer look at amphetamine-induced reverse transport and trafficking of the dopamine and norepinephrine transporters. Mol Neurobiol, 39(2), 73-80, 2009. PMCID:2729543

Binda, F, Dipace, C, Bowton, E, Doughty, SE, Lute, BJ, Fog, JU, Zhang, M, Sen, N, Colbran, RJ, Gnegy, ME, Gether, U, Javitch, JA, Erreger, K, Galli, A. Syntaxin1A Interaction with the Dopamine Transporter Promotes Amphetamine-Induced Dopamine Efflux. Mol Pharmacol, , , 2008. PMCID:2728020

Erreger, K, Grewer, C, Javitch, JA, Galli, A. Currents in response to rapid concentration jumps of amphetamine uncover novel aspects of human dopamine transporter function. J Neurosci, 28(4), 976-89, 2008. PMCID:2459309

Lute, BJ, Khoshbouei, H, Saunders, C, Sen, N, Lin, RZ, Javitch, JA, Galli, A. PI3K signaling supports amphetamine-induced dopamine efflux. Biochem Biophys Res Commun, 372(4), 656-61, 2008. PMCID:2517853

Mazei-Robison, MS, Bowton, E, Holy, M, Schmudermaier, M, Freissmuth, M, Sitte, HH, Galli, A, Blakely, RD. Anomalous dopamine release associated with a human dopamine transporter coding variant. J Neurosci, 28(28), 7040-6, 2008. PMCID:2573963

Dipace, C, Sung, U, Binda, F, Blakely, RD, Galli, A. Amphetamine induces a calcium/calmodulin-dependent protein kinase II-dependent reduction in norepinephrine transporter surface expression linked to changes in syntaxin 1A/transporter complexes. Mol Pharmacol, 71(1), 230-9, 2007.

Wei, Y, Williams, JM, Dipace, C, Sung, U, Javitch, JA, Galli, A, Saunders, C. Dopamine transporter activity mediates amphetamine-induced inhibition of Akt through a Ca2+/calmodulin-dependent kinase II-dependent mechanism. Mol Pharmacol, 71(3), 835-42, 2007.

Williams, JM, Owens, WA, Turner, GH, Saunders, C, Dipace, C, Blakely, RD, France, CP, Gore, JC, Daws, LC, Avison, MJ, Galli, A. Hypoinsulinemia regulates amphetamine-induced reverse transport of dopamine. PLoS Biol, 5(10), 2369-78, 2007. PMCID:2020502

Binda, F, Lute, BJ, Dipace, C, Blakely, RD, Galli, A. The N-terminus of the norepinephrine transporter regulates the magnitude and selectivity of the transporter-associated leak current. Neuropharmacology, 50(3), 354-61, 2006.

Fog, JU, Khoshbouei, H, Holy, M, Owens, WA, Vaegter, CB, Sen, N, Nikandrova, Y, Bowton, E, McMahon, DG, Colbran, RJ, Daws, LC, Sitte, HH, Javitch, JA, Galli, A, Gether, U. Calmodulin kinase II interacts with the dopamine transporter C terminus to regulate amphetamine-induced reverse transport. Neuron, 51(4), 417-29, 2006.

Kahlig, KM, Lute, BJ, Wei, Y, Loland, CJ, Gether, U, Javitch, JA, Galli, A. Regulation of dopamine transporter trafficking by intracellular amphetamine. Mol Pharmacol, 70(2), 542-8, 2006.

Williams, JM, Galli, A. The dopamine transporter: a vigilant border control for psychostimulant action. Handb Exp Pharmacol, (175), 215-32, 2006.

Blakely, Randy D, Defelice, Louis J, Galli, Aurelio. Biogenic amine neurotransmitter transporters: just when you thought you knew them. Physiology (Bethesda), 20(4), 225-31, 2005.

Garcia, B G, Wei, Y, Moron, J A, Lin, R Z, Javitch, J A, Galli, A. Akt is essential for insulin modulation of amphetamine-induced human dopamine transporter cell-surface redistribution. Mol Pharmacol, 68(1), 102-9, 2005.

Kahlig, Kristopher M, Binda, Francesca, Khoshbouei, Habibeh, Blakely, Randy D, McMahon, Douglas G, Javitch, Jonathan A, Galli, Aurelio. Amphetamine induces dopamine efflux through a dopamine transporter channel. Proc Natl Acad Sci U S A, 102(9), 3495-500, 2005. PMCID:549289

Kahlig, Kristopher M, Javitch, Jonathan A, Galli, Aurelio. Amphetamine regulation of dopamine transport. Combined measurements of transporter currents and transporter imaging support the endocytosis of an active carrier. J Biol Chem, 279(10), 8966-75, 2004.

Khoshbouei, Habibeh, Sen, Namita, Guptaroy, Bipasha, Johnson, L ''Aurelle, Lund, David, Gnegy, Margaret E, Galli, Aurelio, Javitch, Jonathan A. N-terminal phosphorylation of the dopamine transporter is required for amphetamine-induced efflux. PLoS Biol, 2(3), E78, 2004. PMCID:368172

Kahlig, KM, Galli, A. Regulation of dopamine transporter function and plasma membrane expression by dopamine, amphetamine, and cocaine. Eur J Pharmacol, 479(1-3), 153-8, 2003.

Khoshbouei, Habibeh, Wang, Hongwei, Lechleiter, James D, Javitch, Jonathan A, Galli, Aurelio. Amphetamine-induced dopamine efflux. A voltage-sensitive and intracellular Na+-dependent mechanism. J Biol Chem, 278(14), 12070-7, 2003.

Sung, U, Apparsundaram, S, Galli, A, Kahlig, KM, Savchenko, V, Schroeter, S, Quick, MW, Blakely, RD. A regulated interaction of syntaxin 1A with the antidepressant-sensitive norepinephrine transporter establishes catecholamine clearance capacity. J Neurosci, 23(5), 1697-709, 2003.

Carvelli, L., Kahlig, K., Moron, J., Ferrer, JV., Lechleiter, JD. Merrill, G., Lafer, E.M., Ballou, LM., Shippenberg, T., Javitch, JA., Lin, RZ and Galli, A. PI 3-Kinase regulation of dopamine transporter activity. J. Neurochem, 81(4), 859-869, 2002.

Saunders, C., Ferrer, J.V., Shi, L., Chen, J., Merrill, G., Lamb, ME., Leeb-Lundberg, L.M.F., Carvelli, L., Javith, J.A., Galli, A. Amphetamine-induced loss of human dopamine transporter activity: an internalization-dependent and cocaine-sensitive mechanism . P.N.A.S., 12(97), 6850-6855, 2000.

Apparsundaram, S, Galli, A, DeFelice, LJ, Hartzell, HC, Blakely, RD. Acute regulation of norepinephrine transport: I. protein kinase C-linked muscarinic receptors influence transport capacity and transporter density in SK-N-SH cells. J Pharmacol Exp Ther, 287(2), 733-43, 1998.

Blakely, RD, Ramamoorthy, S, Schroeter, S, Qian, Y, Apparsundaram, S, Galli, A, DeFelice, LJ. Regulated phosphorylation and trafficking of antidepressant-sensitive serotonin transporter proteins. Biol Psychiatry, 44(3), 169-78, 1998.

DeFelice, LJ, Galli, A. Electrophysiological analysis of transporter function. Adv Pharmacol, 42, 186-90, 1998.

DeFelice, LJ, Galli, A. Fluctuation analysis of norepinephrine and serotonin transporter currents. Methods Enzymol, 296, 578-93, 1998.

Galli, A, Blakely, RD, DeFelice, LJ. Patch-clamp and amperometric recordings from norepinephrine transporters: channel activity and voltage-dependent uptake. Proc Natl Acad Sci U S A, 95(22), 13260-5, 1998. PMCID:23775

Galli, A, Petersen, CI, deBlaquiere, M, Blakely, RD, DeFelice, LJ. Drosophila serotonin transporters have voltage-dependent uptake coupled to a serotonin-gated ion channel. J Neurosci, 17(10), 3401-11, 1997.

Qian, Y, Galli, A, Ramamoorthy, S, Risso, S, DeFelice, LJ, Blakely, RD. Protein kinase C activation regulates human serotonin transporters in HEK-293 cells via altered cell surface expression. J Neurosci, 17(1), 45-57, 1997.

Galli, A, Blakely, RD, DeFelice, LJ. Norepinephrine transporters have channel modes of conduction. Proc Natl Acad Sci U S A, 93(16), 8671-6, 1996. PMCID:38731


Postdoctoral Position Available
Yes

Postdoctoral Position Details
YES

Updated Date
04/22/2012



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