Vanderbilt University School of Medicine

Corbin, Jackie D. , Ph.D.
Professor of Molecular Physiology and Biophysics, Emeritus

Lab Url: N/A

Phone Number: 615-322-4384

Email Address:jackie.corbin@vanderbilt.edu

Corbin, Jackie's picture

Office Address   Mailing Address

766-B Medical Research Building I

766B RRB 0615


Research Keywords
Cyclic AMP, Cyclic GMP, Protein Kinases, Protein Phosphorylation, Phosphodiesterases

Research Specialty
Mechanisms of cyclic GMP mediation of hormone action

Research Description
The naturally occurring cyclic nucleotide, cyclic GMP, mediates the effects of hormones, neurotransmitters, and other agents on many biological processes. Blood flow, blood pressure, nerve transmission, airway distension, and penile erection are but a few such processes. Our research concerns the mechanisms by which cyclic GMP causes these effects. The focus is on two classes of enzymes known to be receptors for cyclic GMP: (1) cyclic GMP-dependent protein kinases and (2) phosphodiesterases.


Studies include incubation of intact cells (coronary artery strips, aorta cells) with hormones, neurotransmitters, and cyclic nucleotide analogs followed by measurements of the physiological responses. The investigations also include identification and purification of isozymic forms, cloning of their cDNAs, and studies of enzyme and regulatory mechanisms by biochemical and molecular biological methods. Changes in the conformations of these proteins caused by cyclic nucleotide binding and phosphorylation are being measured.


Of particular interest are the mechanisms by which cyclic nucleotides bind to the enzymes and stimulate protein phosphorylation or other processes. Deletion mutagenesis and site-directed mutagenesis are being used to identify important structural elements in the regulatory components of these enzymes. The physiological substrates for cyclic GMP-dependent protein kinases are being identified and autophosphorylation of the protein kinases is being examined.

The negative feedback regulation of cellular cyclic nucleotide levels is being studied. A probable target of negative feedback, a cyclic GMP-binding phosphodiesterase, which was discovered in this laboratory, is being characterized, and the probability that this enzyme is modulated by both allosteric regulation and protein phosphorylation is being examined. A possible role for zinc in the catalytic mechanism of the phosphodiesterase is being studied. This phosphodiesterase, also referred to as PDE5, is the site of action of the new male impotence drug Viagra, which causes penile erection.


Publications
Bessay, EP, Zoraghi, R, Blount, MA, Grimes, KA, Beasley, A, Francis, SH, Corbin, JD. Phosphorylation of phosphodiesterase-5 is promoted by a conformational change induced by sildenafil, vardenafil, or tadalafil. Front Biosci, 12, 1899-910, 2007.

Blount, MA, Zoraghi, R, Ke, H, Bessay, EP, Corbin, JD, Francis, SH. A 46-amino acid segment in phosphodiesterase-5 GAF-B domain provides for high vardenafil potency over sildenafil and tadalafil and is involved in phosphodiesterase-5 dimerization. Mol Pharmacol, 70(5), 1822-31, 2006.

Richie-Jannetta, R, Busch, JL, Higgins, KA, Corbin, JD, Francis, SH. Isolated regulatory domains of cGMP-dependent protein kinase Ialpha and Ibeta retain dimerization and native cGMP-binding properties, and undergo isoform-specific conformational changes. J Biol Chem, , , 2006.

Zoraghi, R, Corbin, JD, Francis, SH. Phosphodiesterase-5 Gln817 Is Critical for cGMP, Vardenafil, or Sildenafil Affinity: ITS ORIENTATION IMPACTS cGMP BUT NOT cAMP AFFINITY. J Biol Chem, 281(9), 5553-8, 2006.

Corbin, JD, Beasley, A, Blount, MA, Francis, SH. High lung PDE5: A strong basis for treating pulmonary hypertension with PDE5 inhibitors. Biochem Biophys Res Commun, 334(3), 930-8, 2005.

Francis, SH, Blount, MA, Zoraghi, R, Corbin, JD. Molecular properties of mammalian proteins that interact with cGMP: protein kinases, cation channels, phosphodiesterases, and multi-drug anion transporters. Front Biosci, 10, 2097-117, 2005.

Francis, SH, Corbin, JD. Sildenafil: efficacy, safety, tolerability and mechanism of action in treating erectile dysfunction. Expert Opin Drug Metab Toxicol, 1(2), 283-93, 2005.

Francis, SH, Corbin, JD. Phosphodiesterase-5 inhibition: the molecular biology of erectile function and dysfunction. Urol Clin North Am, 32(4), 419-29, vi, 2005.

Weeks, JL, Blount, MA, Beasley, A, Zoraghi, R, Thomas, MK, Sekhar, KR, Corbin, JD, Francis, SH. Radiolabeled ligand binding to the catalytic or allosteric sites of PDE5 and PDE11. Methods Mol Biol, 307, 239-62, 2005.

Zoraghi, R, Bessay, EP, Corbin, JD, Francis, SH. Structural and functional features in human PDE5A1 regulatory domain that provide for allosteric cGMP binding, dimerization, and regulation. J Biol Chem, 280(12), 12051-63, 2005.

Blount, MA, Beasley, A, Zoraghi, R, Sekhar, KR, Bessay, EP, Francis, SH, Corbin, JD. Binding of tritiated sildenafil, tadalafil, or vardenafil to the phosphodiesterase-5 catalytic site displays potency, specificity, heterogeneity, and cGMP stimulation. Mol Pharmacol, 66(1), 144-52, 2004.

Corbin, JD. Mechanisms of action of PDE5 inhibition in erectile dysfunction. Int J Impot Res, 16 Suppl 1, S4-7, 2004.

Corbin, JD, Beasley, A, Blount, MA, Francis, SH. Vardenafil: structural basis for higher potency over sildenafil in inhibiting cGMP-specific phosphodiesterase-5 (PDE5). Neurochem Int, 45(6), 859-63, 2004.

Kotera, Jun, Francis, Sharron H, Grimes, Kennard A, Rouse, Alfreda, Blount, Mitsi A, Corbin, Jackie D. Allosteric sites of phosphodiesterase-5 sequester cyclic GMP. Front Biosci, 9, 378-86, 2004.

Weeks, JL, Zoraghi, R, Beasley, A, Sekhar, KR, Francis, SH, Corbin, JD. High biochemical selectivity of tadalafil, sildenafil and vardenafil for human phosphodiesterase 5A1 (PDE5) over PDE11A4 suggests the absence of PDE11A4 cross-reaction in patients. Int J Impot Res, 17(1), 5-9, 2004.

Zhu, CB, Hewlett, WA, Francis, SH, Corbin, JD, Blakely, RD. Stimulation of serotonin transport by the cyclic GMP phosphodiesterase-5 inhibitor sildenafil. Eur J Pharmacol, 504(1-2), 1-6, 2004.

Zoraghi, Roya, Corbin, Jackie D, Francis, Sharron H. Properties and functions of GAF domains in cyclic nucleotide phosphodiesterases and other proteins. Mol Pharmacol, 65(2), 267-78, 2004.

Corbin, Jackie D, Blount, Mitsi A, Weeks, James L, Beasley, Alfreda, Kuhn, Karl P, Ho, Yew S J, Saidi, Layla F, Hurley, James H, Kotera, Jun, Francis, Sharron H. [3H]sildenafil binding to phosphodiesterase-5 is specific, kinetically heterogeneous, and stimulated by cGMP. Mol Pharmacol, 63(6), 1364-72, 2003.

Corbin, Jackie D, Francis, Sharron H. Molecular biology and pharmacology of PDE-5-inhibitor therapy for erectile dysfunction. J Androl, 24(6 Suppl), S38-41, 2003.

Francis, S H, Sekhar, K Raja, Rouse, A B, Grimes, K A, Corbin, J D. Single step isolation of sildenafil from commercially available Viagra tablets. Int J Impot Res, 15(5), 369-72, 2003.

Francis, Sharron H, Corbin, Jackie D. Molecular mechanisms and pharmacokinetics of phosphodiesterase-5 antagonists. Curr Urol Rep, 4(6), 457-65, 2003.

Kotera, Jun, Grimes, Kennard A, Corbin, Jackie D, Francis, Sharron H. cGMP-dependent protein kinase protects cGMP from hydrolysis by phosphodiesterase-5. Biochem J, 372(Pt 2), 419-26, 2003. PMCID:1223414

Richie-Jannetta, Robyn, Francis, Sharron H, Corbin, Jackie D. Dimerization of cGMP-dependent protein kinase Ibeta is mediated by an extensive amino-terminal leucine zipper motif, and dimerization modulates enzyme function. J Biol Chem, 278(50), 50070-9, 2003.

Busch, Jennifer L, Bessay, Emmanuel P, Francis, Sharron H, Corbin, Jackie D. A conserved serine juxtaposed to the pseudosubstrate site of type I cGMP-dependent protein kinase contributes strongly to autoinhibition and lower cGMP affinity. J Biol Chem, 277(37), 34048-54, 2002.

Corbin, J D, Francis, S H. Pharmacology of phosphodiesterase-5 inhibitors. Int J Clin Pract, 56(6), 453-9, 2002.

Corbin, Jackie D, Francis, Sharron H, Webb, David J. Phosphodiesterase type 5 as a pharmacologic target in erectile dysfunction. Urology, 60(2 Suppl 2), 4-11, 2002.

Dzhura, Igor, Wu, Yuejin, Colbran, Roger J, Corbin, Jackie D, Balser, Jeffrey R, Anderson, Mark E. Cytoskeletal disrupting agents prevent calmodulin kinase, IQ domain and voltage-dependent facilitation of L-type Ca2+ channels. J Physiol, 545(Pt 2), 399-406, 2002. PMCID:2290681

Francis, Sharron H, Bessay, Emmanuel P, Kotera, Jun, Grimes, Kennard A, Liu, Li, Thompson, W Joseph, Corbin, Jackie D. Phosphorylation of isolated human phosphodiesterase-5 regulatory domain induces an apparent conformational change and increases cGMP binding affinity. J Biol Chem, 277(49), 47581-7, 2002.

Francis, Sharron H, Poteet-Smith, Celeste, Busch, Jennifer L, Richie-Jannetta, Robyn, Corbin, Jackie D. Mechanisms of autoinhibition in cyclic nucleotide-dependent protein kinases. Front Biosci, 7, d580-92, 2002.

Francis, S H, Turko, I V, Corbin, J D. Cyclic nucleotide phosphodiesterases: relating structure and function. Prog Nucleic Acid Res Mol Biol, 65, 1-52, 2001.

Gopal, V K, Francis, S H, Corbin, J D. Allosteric sites of phosphodiesterase-5 (PDE5). A potential role in negative feedback regulation of cGMP signaling in corpus cavernosum. Eur J Biochem, 268(11), 3304-12, 2001.

Corbin, J D, Turko, I V, Beasley, A, Francis, S H. Phosphorylation of phosphodiesterase-5 by cyclic nucleotide-dependent protein kinase alters its catalytic and allosteric cGMP-binding activities. Eur J Biochem, 267(9), 2760-7, 2000.

Francis, S H, Turko, I V, Grimes, K A, Corbin, J D. Histidine-607 and histidine-643 provide important interactions for metal support of catalysis in phosphodiesterase-5. Biochemistry, 39(31), 9591-6, 2000.

Smith, J A, Reed, R B, Francis, S H, Grimes, K, Corbin, J D. Distinguishing the roles of the two different cGMP-binding sites for modulating phosphorylation of exogenous substrate (heterophosphorylation) and autophosphorylation of cGMP-dependent protein kinase. J Biol Chem, 275(1), 154-8, 2000.

Corbin, J D, Francis, S H. Cyclic GMP phosphodiesterase-5: target of sildenafil. J Biol Chem, 274(20), 13729-32, 1999.

Fink, T L, Francis, S H, Beasley, A, Grimes, K A, Corbin, J D. Expression of an active, monomeric catalytic domain of the cGMP-binding cGMP-specific phosphodiesterase (PDE5). J Biol Chem, 274(49), 34613-20, 1999.

Francis, S H, Corbin, J D. Cyclic nucleotide-dependent protein kinases: intracellular receptors for cAMP and cGMP action. Crit Rev Clin Lab Sci, 36(4), 275-328, 1999.

Turko, I V, Ballard, S A, Francis, S H, Corbin, J D. Inhibition of cyclic GMP-binding cyclic GMP-specific phosphodiesterase (Type 5) by sildenafil and related compounds. Mol Pharmacol, 56(1), 124-30, 1999.

Turko, I V, Francis, S H, Corbin, J D. Studies of the molecular mechanism of discrimination between cGMP and cAMP in the allosteric sites of the cGMP-binding cGMP-specific phosphodiesterase (PDE5). J Biol Chem, 274(41), 29038-41, 1999.

Corbin, J.D., Beasley, A., Turko, I.V., Haik, T.L., Mangum, K.A., Wells, J.N., Francis, S.H., and Sekhar, K.R. (1998) A photoaffinity probe covalently modifies the catalytic site of the cGMP-binding phosphodiesterase (PDE5). Cell Biochem. and Biophys. 29:145-157.

Granovsky, A.E., Natochin, M., McEntaffer, R., Haik, T.L., Francis, S.H., Corbin, J.D., and Artemyev, N.O. (1998) Probing domain functions of chimeric PDE6a?/PDE5 cGMP-phosphodiesterase. J. Biol. Chem. 273:24485-24490.

Loughney, K., Hill, T.R., Florio, V.A., Uher, L., Rosman, G.J., Wolda, S.L., Jones, B.A., Howard, M.L., McAllister-Lucas, L.M., Sonnenberg, W.K., Francis, S.H., Corbin, J.D., Beavo, J.A., and Ferguson, K. (1998) Isolation and characterization of cDNAs encoding PDE5A, a human cGMP-binding, cGMP-specific 3',5'-cyclic nucleotide phosphodiesterase. Gene 216:139-147

Turko, I.V., Francis, S.H., and Corbin, J.D. (1998) The potential roles of conserved amino acids in the catalytic domain of the cGMP-binding cGMP-specific phosphodiesterase (PDE5). J. Biol. Chem., 273:6460-6466.

Chu, D M, Francis, S H, Thomas, J W, Maksymovitch, E A, Fosler, M, Corbin, J D. Activation by autophosphorylation or cGMP binding produces a similar apparent conformational change in cGMP-dependent protein kinase. J Biol Chem, 273(23), 14649-56, 1998.

Corbin, J D, Beasley, A, Turko, I V, Haik, T L, Mangum, K A, Wells, J N, Francis, S H, Sekhar, K R. A photoaffinity probe covalently modifies the catalytic site of the cGMP-binding cGMP-specific phosphodiesterase (PDE-5). Cell Biochem Biophys, 29(1-2), 145-57, 1998.

Francis, S H, Chu, D M, Thomas, M K, Beasley, A, Grimes, K, Busch, J L, Turko, I V, Haik, T L, Corbin, J D. Ligand-induced conformational changes in cyclic nucleotide phosphodiesterases and cyclic nucleotide-dependent protein kinases. Methods, 14(1), 81-92, 1998.

Turko, I V, Francis, S H, Corbin, J D. Binding of cGMP to both allosteric sites of cGMP-binding cGMP-specific phosphodiesterase (PDE5) is required for its phosphorylation. Biochem J, 329 ( Pt 3), 505-10, 1998. PMCID:1219070

Turko, I V, Francis, S H, Corbin, J D. Potential roles of conserved amino acids in the catalytic domain of the cGMP-binding cGMP-specific phosphodiesterase. J Biol Chem, 273(11), 6460-6, 1998.

Turko, I V, Francis, S H, Corbin, J D. Hydropathic analysis and mutagenesis of the catalytic domain of the cGMP-binding cGMP-specific phosphodiesterase (PDE5). cGMP versus cAMP substrate selectivity. Biochemistry, 37(12), 4200-5, 1998.

Wyatt, T A, Naftilan, A J, Francis, S H, Corbin, J D. ANF elicits phosphorylation of the cGMP phosphodiesterase in vascular smooth muscle cells. Am J Physiol, 274(2 Pt 2), H448-55, 1998.

Poteet-Smith, C.E., Shabb, J.B., Francis, S.H., and Corbin, J.D. (1997) Identification of critical determinants for autoinhibition in the pseudosubstrate region of type Ia cAMP-dependent protein kinase. J. Biol. Chem. 272:379-388.

Wyatt, T.A., Naftilan, A.J., Francis, S.H., and Corbin, J.D.(1997) ANF elicits phosphorylation of the cGMP phosphodiesterase (PDE5) in vascular smooth muscle cells. Am. J. Physiol. 273:H448-H455.

Zhao, J. , Trewhella, J., Corbin, J.D., Francis, S.H., Mitchell, R., Brushia, R., and Walsh, D. (1997) Progressive cyclic nucleotide-induced conformational changes in the cGMP-dependent protein kinase studied by small angle x-ray scattering in solution. J. Biol. Chem. 272:31929-31936.

Chu, D M, Corbin, J D, Grimes, K A, Francis, S H. Activation by cyclic GMP binding causes an apparent conformational change in cGMP-dependent protein kinase. J Biol Chem, 272(50), 31922-8, 1997.

Poteet-Smith, C E, Corbin, J D, Francis, S H. The pseudosubstrate sequences alone are not sufficient for potent autoinhibition of cAMP- and cGMP-dependent protein kinases as determined by synthetic peptide analysis. Adv Second Messenger Phosphoprotein Res, 31, 219-35, 1997.

Poteet-Smith, C E, Shabb, J B, Francis, S H, Corbin, J D. Identification of critical determinants for autoinhibition in the pseudosubstrate region of type I alpha cAMP-dependent protein kinase. J Biol Chem, 272(1), 379-88, 1997.

Reed, R B, Sandberg, M, Jahnsen, T, Lohmann, S M, Francis, S H, Corbin, J D. Structural order of the slow and fast intrasubunit cGMP-binding sites of type I alpha cGMP-dependent protein kinase. Adv Second Messenger Phosphoprotein Res, 31, 205-17, 1997.

Francis, S.H. and Corbin, J.D. (1996) Cyclic AMP and cyclic GMP in cell signaling. In: Signal Transduction, C.-H. Heldin and M. Purton, Eds. Chapman and Hall, London, pp. 223-240.

Sekhar, K.R., Grondin, P., Francis, S.H., and Corbin, J.D. (1996) Design and synthesis of xanthines and cGMP analogues as potent inhibitors of PDE5. In: Phosphodiesterase Inhibitors, C. Schudt, G. Dent, and K.F.

Yan, X., Lawrence, D.S., Corbin, J.D., and Francis, S.H. (1996) Distinguishing between a mitogenic and two closely related nonmitogenic protein kinases. J. Am. Chem. Soc. 118:11684-11685

Francis, S H, Smith, J A, Colbran, J L, Grimes, K, Walsh, K A, Kumar, S, Corbin, J D. Arginine 75 in the pseudosubstrate sequence of type Ibeta cGMP-dependent protein kinase is critical for autoinhibition, although autophosphorylated serine 63 is outside this sequence. J Biol Chem, 271(34), 20748-55, 1996.

Reed, R B, Sandberg, M, Jahnsen, T, Lohmann, S M, Francis, S H, Corbin, J D. Fast and slow cyclic nucleotide-dissociation sites in cAMP-dependent protein kinase are transposed in type Ibeta cGMP-dependent protein kinase. J Biol Chem, 271(29), 17570-5, 1996.

Smith, J A, Francis, S H, Walsh, K A, Kumar, S, Corbin, J D. Autophosphorylation of type Ibeta cGMP-dependent protein kinase increases basal catalytic activity and enhances allosteric activation by cGMP or cAMP. J Biol Chem, 271(34), 20756-62, 1996.

Turko, I V, Haik, T L, McAllister-Lucas, L M, Burns, F, Francis, S H, Corbin, J D. Identification of key amino acids in a conserved cGMP-binding site of cGMP-binding phosphodiesterases. A putative NKXnD motif for cGMP binding. J Biol Chem, 271(36), 22240-4, 1996.

McAllister-Lucas, L M, Haik, T L, Colbran, J L, Sonnenburg, W K, Seger, D, Turko, I V, Beavo, J A, Francis, S H, Corbin, J D. An essential aspartic acid at each of two allosteric cGMP-binding sites of a cGMP-specific phosphodiesterase. J Biol Chem, 270(51), 30671-9, 1995.

Francis, S H, Colbran, J L, McAllister-Lucas, L M, Corbin, J D. Zinc interactions and conserved motifs of the cGMP-binding cGMP-specific phosphodiesterase suggest that it is a zinc hydrolase. J Biol Chem, 269(36), 22477-80, 1994.

Francis, S H, Corbin, J D. Structure and function of cyclic nucleotide-dependent protein kinases. Annu Rev Physiol, 56, 237-72, 1994.

Francis, S H, Corbin, J D. Progress in understanding the mechanism and function of cyclic GMP-dependent protein kinase. Adv Pharmacol, 26, 115-70, 1994.

Corbin, J D, Woodall, C C, Colbran, J L, McAllister, L M, Sekhar, K R, Francis, S H. Identifying protein kinases in crude extracts that phosphorylate cyclic GMP-binding cyclic-GMP specific phosphodiesterase. Agents Actions Suppl, 43, 27-33, 1993.

McAllister-Lucas, L M, Sonnenburg, W K, Kadlecek, A, Seger, D, Trong, H L, Colbran, J L, Thomas, M K, Walsh, K A, Francis, S H, Corbin, J D. The structure of a bovine lung cGMP-binding, cGMP-specific phosphodiesterase deduced from a cDNA clone. J Biol Chem, 268(30), 22863-73, 1993.

Smith, J A, Francis, S H, Corbin, J D. Autophosphorylation: a salient feature of protein kinases. Mol Cell Biochem, 127-128, 51-70, 1993.

Colbran, J L, Francis, S H, Leach, A B, Thomas, M K, Jiang, H, McAllister, L M, Corbin, J D. A phenylalanine in peptide substrates provides for selectivity between cGMP- and cAMP-dependent protein kinases. J Biol Chem, 267(14), 9589-94, 1992.

Colbran, J L, Roach, P J, Fiol, C J, Dixon, J E, Andrisani, O M, Corbin, J D. cAMP-dependent protein kinase, but not the cGMP-dependent enzyme, rapidly phosphorylates delta-CREB, and a synthetic delta-CREB peptide. Biochem Cell Biol, 70(10-11), 1277-82, 1992.

Jiang, H, Colbran, J L, Francis, S H, Corbin, J D. Direct evidence for cross-activation of cGMP-dependent protein kinase by cAMP in pig coronary arteries. J Biol Chem, 267(2), 1015-9, 1992.

Jiang, H, Shabb, J B, Corbin, J D. Cross-activation: overriding cAMP/cGMP selectivities of protein kinases in tissues. Biochem Cell Biol, 70(12), 1283-9, 1992.

Sekhar, K R, Hatchett, R J, Shabb, J B, Wolfe, L, Francis, S H, Wells, J N, Jastorff, B, Butt, E, Chakinala, M M, Corbin, J D. Relaxation of pig coronary arteries by new and potent cGMP analogs that selectively activate type I alpha, compared with type I beta, cGMP-dependent protein kinase. Mol Pharmacol, 42(1), 103-8, 1992.

Shabb, J B, Corbin, J D. Cyclic nucleotide-binding domains in proteins having diverse functions. J Biol Chem, 267(9), 5723-6, 1992.

Thomas, M K, Francis, S H, Beebe, S J, Gettys, T W, Corbin, J D. Partial mapping of cyclic nucleotide sites and studies of regulatory mechanisms of phosphodiesterases using cyclic nucleotide analogues. Adv Second Messenger Phosphoprotein Res, 25, 45-53, 1992.

Jiang, H, Corbin, J D. Effect of epinephrine or cAMP on cAMP-bound protein kinase holoenzymes in rat heart. Am J Physiol, 260(3 Pt 2), H722-9, 1991.

Shabb, J B, Buzzeo, B D, Ng, L, Corbin, J D. Mutating protein kinase cAMP-binding sites into cGMP-binding sites. Mechanism of cGMP selectivity. J Biol Chem, 266(36), 24320-6, 1991.

Corbin, J D, Thomas, M K, Wolfe, L, Shabb, J B, Woodford, T A, Francis, S H. New insights into cGMP action. Adv Second Messenger Phosphoprotein Res, 24, 411-8, 1990.

Redmon, J B, Gettys, T W, Sheorain, V S, Corbin, J D, Taylor, I L. Failure of insulin to antagonize cAMP-mediated glycogenolysis in rat ventricular cardiomyocytes. Am J Physiol, 258(5 Pt 1), E871-7, 1990.

Shabb, J B, Ng, L, Corbin, J D. One amino acid change produces a high affinity cGMP-binding site in cAMP-dependent protein kinase. J Biol Chem, 265(27), 16031-4, 1990.

Thomas, M K, Francis, S H, Corbin, J D. Substrate- and kinase-directed regulation of phosphorylation of a cGMP-binding phosphodiesterase by cGMP. J Biol Chem, 265(25), 14971-8, 1990.

Beebe, S J, Segaloff, D L, Burks, D, Beasley-Leach, A, Limbird, L E, Corbin, J D. Evidence that cyclic adenosine 3',5'-monophosphate-dependent protein kinase activation causes pig ovarian granulosa cell differentiation, including increases in two type II subclasses of this kinase. Biol Reprod, 41(2), 295-307, 1989.

Wolfe, L, Corbin, J D. Cyclic nucleotides and disease. Curr Opin Cell Biol, 1(2), 215-9, 1989.

Wolfe, L, Corbin, J D, Francis, S H. Characterization of a novel isozyme of cGMP-dependent protein kinase from bovine aorta. J Biol Chem, 264(13), 7734-41, 1989.

Wolfe, L, Francis, S H, Corbin, J D. Properties of a cGMP-dependent monomeric protein kinase from bovine aorta. J Biol Chem, 264(7), 4157-62, 1989.

Woodford, T A, Correll, L A, McKnight, G S, Corbin, J D. Expression and characterization of mutant forms of the type I regulatory subunit of cAMP-dependent protein kinase. The effect of defective cAMP binding on holoenzyme activation. J Biol Chem, 264(22), 13321-8, 1989.

Corbin, J D, Cobb, C E, Beebe, S J, Granner, D K, Koch, S R, Gettys, T W, Blackmore, P F, Francis, S H, Wells, J N. Mechanism and function of cAMP- and cGMP-dependent protein kinases. Adv Second Messenger Phosphoprotein Res, 21, 75-86, 1988.

Francis, S H, Noblett, B D, Todd, B W, Wells, J N, Corbin, J D. Relaxation of vascular and tracheal smooth muscle by cyclic nucleotide analogs that preferentially activate purified cGMP-dependent protein kinase. Mol Pharmacol, 34(4), 506-17, 1988.

Francis, S H, Woodford, T A, Wolfe, L, Corbin, J D. Types I alpha and I beta isozymes of cGMP-dependent protein kinase: alternative mRNA splicing may produce different inhibitory domains. Second Messengers Phosphoproteins, 12(5-6), 301-10, 1988.

Gettys, T W, Blackmore, P F, Corbin, J D. An assessment of phosphodiesterase activity in situ after treatment of hepatocytes with hormones. Am J Physiol, 254(4 Pt 1), E449-53, 1988.

Gettys, T W, Vine, A J, Simonds, M F, Corbin, J D. Activation of the particulate low Km phosphodiesterase of adipocytes by addition of cAMP-dependent protein kinase. J Biol Chem, 263(21), 10359-63, 1988.

Beebe, S J, Koch, S R, Chu, D T, Corbin, J D, Granner, D K. Regulation of phosphoenolpyruvate carboxykinase gene transcription in H4IIE hepatoma cells: evidence for a primary role of the catalytic subunit of 3',5'-cyclic adenosine monophosphate-dependent protein kinase. Mol Endocrinol, 1(9), 639-47, 1987.

Cobb, C E, Beth, A H, Corbin, J D. Purification and characterization of an inactive form of cAMP-dependent protein kinase containing bound cAMP. J Biol Chem, 262(34), 16566-74, 1987.

Wolfe, L, Francis, S H, Landiss, L R, Corbin, J D. Interconvertible cGMP-free and cGMP-bound forms of cGMP-dependent protein kinase in mammalian tissues. J Biol Chem, 262(35), 16906-13, 1987.


Postdoctoral Position Available
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Postdoctoral Position Details
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Updated Date
04/24/2007



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