Vanderbilt University School of Medicine

Exton, John H. , M.D., Ph.D.
Professor of Molecular Physiology and Biophysics
Professor of Pharmacology

Lab Url: N/A

Phone Number: 615-322-6494

Email Address:john.exton@vanderbilt.edu

Exton, John's picture

Office Address   Mailing Address

831B Light Hall

831B Light Hall 0295


Research Keywords
Phospholipase, Hormones, Growth Factors, Signal Transduction, G Proteins

Research Specialty
Mechanisms of cell signaling

Research Description
Many hormones, neurotransmitters and growth factors produce their physiological actions by stimulating the breakdown of certain phospholipids in their target cells. This leads to an increase in the concentration of Ca2+ ions and changes in lipid messenger molecules that are responsible for the physiological effects observed. We are studying the phospholipase isozymes that are specifically involved in agonist-stimulated phospholipid breakdown and also the G proteins, protein kinases and other factors involved in their regulation. The structural elements required for catalysis and the domains and mechanisms of interaction of the phopholipases and their regulatory proteins are being defined. We are also exploring the molecular mechanisms by which hormones and growth factors stimulate phospholipid breakdown in intact cells, in particular, those mechanisms involving large and small G proteins and protein tyrosine and serine-threonine kinases. We are also examining the roles played by some of the products of phospholipid breakdown (phosphatidic acid and diacylglycerol) in generating physiological responses. The approaches involve the generation of mutant forms of the enzymes and regulatory proteins that are constitutively active or function as dominant negative forms, and the use of cells and animals in which the proteins are selectively depleted. This information is aimed at helping us understand how some of the important physiological reponses to hormones, neurotransmitters and growth factors are generated.


Publications
Couvillon, AD, Exton, JH. Role of heterotrimeric G-proteins in lysophosphatidic acid-mediated neurite retraction by RhoA-dependent and -independent mechanisms in N1E-115 cells. Cell Signal, 18(5), 715-28, 2006.

Henage, LG, Exton, JH, Brown, HA. Kinetic analysis of a mammalian phospholipase D: allosteric modulation by monomeric GTPases, protein kinase C, and polyphosphoinositides. J Biol Chem, 281(6), 3408-17, 2006.

Hu, P, Han, Z, Couvillon, AD, Kaufman, RJ, Exton, JH. Autocrine tumor necrosis factor alpha links endoplasmic reticulum stress to the membrane death receptor pathway through IRE1alpha-mediated NF-kappaB activation and down-regulation of TRAF2 expression. Mol Cell Biol, 26(8), 3071-84, 2006. PMCID:1446932

Buchanan, FG, McReynolds, M, Couvillon, A, Kam, Y, Holla, VR, Dubois, RN, Exton, JH. Requirement of phospholipase D1 activity in H-RasV12-induced transformation. Proc Natl Acad Sci U S A, 102(5), 1638-42, 2005. PMCID:547811

Chen, JS, Exton, JH. Sites on phospholipase D2 phosphorylated by PKCalpha. Biochem Biophys Res Commun, 333(4), 1322-6, 2005.

Hiroyama, M, Exton, JH. Studies of the roles of ADP-ribosylation factors and phospholipase D in phorbol ester-induced membrane ruffling. J Cell Physiol, 202(2), 608-22, 2005.

Hiroyama, M, Exton, JH. Localization and regulation of phospholipase D2 by ARF6. J Cell Biochem, 95(1), 149-64, 2005.

Ho, WT, Xie, Z, Zhao, ZJ, Exton, JH. Tyrosine phosphorylation of phospholipase D1 by v-Src does not per se result in activation. Cell Signal, 17(6), 691-9, 2005.

Hu, T, Exton, JH. A point mutation at phenylalanine 663 abolishes protein kinase C alpha''s ability to translocate to the perinuclear region and activate phospholipase D1. Biochem Biophys Res Commun, 333(3), 750-3, 2005.

Hu, T, Exton, JH. 1-Butanol interferes with phospholipase D1 and protein kinase Calpha association and inhibits phospholipase D1 basal activity. Biochem Biophys Res Commun, 327(4), 1047-51, 2005.

Kook, S, Exton, JH. Identification of interaction sites of protein kinase Calpha on phospholipase D1. Cell Signal, 17(11), 1423-32, 2005.

Chen, JS, Exton, JH. Regulation of phospholipase D2 activity by protein kinase C alpha. J Biol Chem, 279(21), 22076-83, 2004.

Hu, T, Exton, JH. Protein kinase Calpha translocates to the perinuclear region to activate phospholipase D1. J Biol Chem, 279(34), 35702-8, 2004.

Kam, Yoonseok, Exton, John H. Role of phospholipase D in the activation of protein kinase D by lysophosphatidic acid. Biochem Biophys Res Commun, 315(1), 139-43, 2004.

Kam, Yoonseok, Exton, John H. Role of phospholipase D1 in the regulation of mTOR activity by lysophosphatidic acid. FASEB J, 18(2), 311-9, 2004.

Ho, Wan-Ting, Exton, John H, Williger, Ben-Tsion. Arfaptin 1 inhibits ADP-ribosylation factor-dependent matrix metalloproteinase-9 secretion induced by phorbol ester in HT 1080 fibrosarcoma cells. FEBS Lett, 537(1-3), 91-5, 2003.

Hu, Tianhui, Exton, John H. Mechanisms of regulation of phospholipase D1 by protein kinase Calpha. J Biol Chem, 278(4), 2348-55, 2003.

Exton, J H. Phospholipase D-structure, regulation and function. Rev Physiol Biochem Pharmacol, 144, 1-94, 2002.

Exton, John H. Regulation of phospholipase D. FEBS Lett, 531(1), 58-61, 2002.

Kam, Yoonseok, Exton, John H. Dimerization of phospholipase d isozymes. Biochem Biophys Res Commun, 290(1), 375-80, 2002.

Xie, Z., Ho, W.-T., Spellmlan, R., Cai, S. and Exton, J.H. Mechanisms of regulation of phospholipase D1 and D2 by the heterotrimeric G proteins G(13) and G(q). J. Biol. Chem., 277, 11979-11986, 2002.

Xie, Zhi, Ho, Wan-Ting, Exton, John H. Functional implications of post-translational modifications of phospholipases D1 and D2. Biochim Biophys Acta, 1580(1), 9-21, 2002.

Xie, Zhi, Ho, Wan-Ting, Spellman, Rachel, Cai, Songmin, Exton, John H. Mechanisms of regulation of phospholipase D1 and D2 by the heterotrimeric G proteins G13 and Gq. J Biol Chem, 277(14), 11979-86, 2002.

Xie, Zhi, Kim, Ha Kun, Exton, John H. Expression and characterization of rat brain phospholipase D. Methods Enzymol, 345, 255-64, 2002.

Cai, S, Exton, J H. Determination of interaction sites of phospholipase D1 for RhoA. Biochem J, 355(Pt 3), 779-85, 2001. PMCID:1221795

Kam, Y, Exton, J H. Phospholipase D activity is required for actin stress fiber formation in fibroblasts. Mol Cell Biol, 21(12), 4055-66, 2001. PMCID:87067

Kam, Y. and Exton, J.H. Phospholipase D activity as required for actin stress fiber formation in fibroblasts. Mol. Cell. Biol., 21, 4055-4066, 2001.

Shin, O H, Couvillon, A D, Exton, J H. Arfophilin is a common target of both class II and class III ADP-ribosylation factors. Biochemistry, 40(36), 10846-52, 2001.

Shin, O H, Exton, J H. Differential binding of arfaptin 2/POR1 to ADP-ribosylation factors and Rac1. Biochem Biophys Res Commun, 285(5), 1267-73, 2001.

Xie, Z, Ho, W T, Exton, J H. Requirements and effects of palmitoylation of rat PLD1. J Biol Chem, 276(12), 9383-91, 2001.

Xie, Z., Ho, W.-T. and Exton, J.H. Requirements and effects of palmitoylation of rat PLD1. J. Biol. Chem, 276, 9383-9391, 2001.

Buchanan, F G, Elliot, C M, Gibbs, M, Exton, J H. Translocation of the Rac1 guanine nucleotide exchange factor Tiam1 induced by platelet-derived growth factor and lysophosphatidic acid. J Biol Chem, 275(13), 9742-8, 2000.

Buchanan, F.G., Elliott, C.M., Gibbs M. and Exton, J.H. Translocation of the Rac1 guanine nucleotide exchange factor and lysophosphatidic acid. J. Biol. Chem, 275, 9742-9748, 2000.

Exton, J H. Phospholipase D. Ann N Y Acad Sci, 905, 61-8, 2000.

Hess, J A, Buchanan, F G, Ryder, S, Exton, J H. Altered activation of phospholipase D by lysophosphatidic acid and endothelin-1 in mouse embryo fibroblasts lacking phospholipase C-gamma1. Cell Signal, 12(1), 37-45, 2000.

Jin, S, Exton, J H. Activation of RhoA by association of Galpha(13) with Dbl. Biochem Biophys Res Commun, 277(3), 718-21, 2000.

Xie, Z, Ho, W T, Exton, J H. Conserved amino acids at the C-terminus of rat phospholipase D1 are essential for enzymatic activity. Eur J Biochem, 267(24), 7138-46, 2000.

Xie, Z, Ho, W T, Exton, J H. Association of the N- and C-terminal domains of phospholipase D. Contribution of the conserved HKD motifs to the interaction and the requirement of the association for Ser/Thr phosphorylation of the enzyme. J Biol Chem, 275(32), 24962-9, 2000.

Exton, J H. Regulation of phospholipase D. Biochim Biophys Acta, 1439(2), 121-33, 1999.

Fleming, I N, Elliott, C M, Buchanan, F G, Downes, C P, Exton, J H. Ca2+/calmodulin-dependent protein kinase II regulates Tiam1 by reversible protein phosphorylation. J Biol Chem, 274(18), 12753-8, 1999.

Fleming, I.N., Elliott, C.M., Buchanan, F.G. and Exton, J.H. Ca2+calmodulin-dependent protein kinase II regulates Tiam1 by reversible protein phosphorylation. J. Biol. Chem, 274, 12753-12758, 1999.

Shin, O H, Ross, A H, Mihai, I, Exton, J H. Identification of arfophilin, a target protein for GTP-bound class II ADP-ribosylation factors. J Biol Chem, 274(51), 36609-15, 1999.

Williger, B T, Ho, W T, Exton, J H. Phospholipase D mediates matrix metalloproteinase-9 secretion in phorbol ester-stimulated human fibrosarcoma cells. J Biol Chem, 274(2), 735-8, 1999.

Williger, B T, Ostermann, J, Exton, J H. Arfaptin 1, an ARF-binding protein, inhibits phospholipase D and endoplasmic reticulum/Golgi protein transport. FEBS Lett, 443(2), 197-200, 1999.

Williger, B T, Provost, J J, Ho, W T, Milstine, J, Exton, J H. Arfaptin 1 forms a complex with ADP-ribosylation factor and inhibits phospholipase D. FEBS Lett, 454(1-2), 85-9, 1999.

Bae, C D, Min, D S, Fleming, I N, Exton, J H. Determination of interaction sites on the small G protein RhoA for phospholipase D. J Biol Chem, 273(19), 11596-604, 1998.

Bae, C.D., Min, D.S., Fleming, I.N. and Exton, J.H. Determination of interaction sites on the small G protein RhoA for phospholipase D. J. Biol. Chem, 273, 11596-11604, 1998.

Exton, J H. Phospholipase D. Biochim Biophys Acta, 1436(1-2), 105-15, 1998.

Exton, J H. Small GTPases minireview series. J Biol Chem, 273(32), 19923, 1998.

Fleming, I N, Elliott, C M, Exton, J H. Phospholipase C-gamma, protein kinase C and Ca2+/calmodulin-dependent protein kinase II are involved in platelet-derived growth factor-induced phosphorylation of Tiam1. FEBS Lett, 429(3), 229-33, 1998.

Guillemain, I, Exton, J H. Role of rho proteins in agonist regulation of phospholipase D in HL-60 cells. Biochim Biophys Acta, 1405(1-3), 161-70, 1998.

Hess, J A, Ji, Q S, Carpenter, G, Exton, J H. Analysis of platelet-derived growth factor-induced phospholipase D activation in mouse embryo fibroblasts lacking phospholipase C-gamma1. J Biol Chem, 273(32), 20517-24, 1998.

Min, D S, Exton, J H. Phospholipase D is associated in a phorbol ester-dependent manner with protein kinase C-alpha and with a 220-kDa protein which is phosphorylated on serine and threonine. Biochem Biophys Res Commun, 248(3), 533-7, 1998.

Min, D S, Kim, E G, Exton, J H. Involvement of tyrosine phosphorylation and protein kinase C in the activation of phospholipase D by H2O2 in Swiss 3T3 fibroblasts. J Biol Chem, 273(45), 29986-94, 1998.

Min, D S, Park, S K, Exton, J H. Characterization of a rat brain phospholipase D isozyme. J Biol Chem, 273(12), 7044-51, 1998.

Min, D.S., Kim, E.-G. and Exton, J.H. Involvement of tyrosine phosphorylation and protein kinase C in the activation of phospholipase D by H2O2 in Swiss 3T3 fibroblasts. J. Biol. Chem, 273, 29986-29994, 1998.

Min, D.S., Park, S.-K. and Exton, J.H. Characterization of a rat brain phospholipase D isozyme. J. Biol. Chem, 273, 7044-7051, 1998.

Park, S K, Min, D S, Exton, J H. Definition of the protein kinase C interaction site of phospholipase D. Biochem Biophys Res Commun, 244(2), 364-7, 1998.

Plonk, S G, Park, S K, Exton, J H. The alpha-subunit of the heterotrimeric G protein G13 activates a phospholipase D isozyme by a pathway requiring Rho family GTPases. J Biol Chem, 273(9), 4823-6, 1998.

Plonk, S. G., Park, S. -K. and Exton, J. H. The a-subunit of the heterotrimeric G protein G13 activates a phospholipase D isozyme by a pathway requiring Rho-family GTPases. J. Biol. Chem., 273, 4823-4836, 1998.

Tsai, S-C., Adamik, R., Hong, J-X., Moss, J., Vaughan, M., Kanoh, H. and Exton, J.H. Effects of arfaptin 1 on guanine nucleotide-dependent activation of phospholipase D and cholera toxin by ARF. J. Biol. Chem, 273, 20697-20701, 1998.

Xie, Z, Ho, W T, Exton, J H. Association of N- and C-terminal domains of phospholipase D is required for catalytic activity. J Biol Chem, 273(52), 34679-82, 1998.

Xie, Z., Ho, W.-T. and Exton, J.H. Association of N- and C-terminal domains of phospholipase D is required for catalytic activity. J. Biol. Chem., 273, 34679-34682, 1998.

Exton, J H. New developments in phospholipase D. J Biol Chem, 272(25), 15579-82, 1997.

Exton, J H. Phospholipase D: enzymology, mechanisms of regulation, and function. Physiol Rev, 77(2), 303-20, 1997.

Exton, J H. Cell signalling through guanine-nucleotide-binding regulatory proteins (G proteins) and phospholipases. Eur J Biochem, 243(1-2), 10-20, 1997.

Fleming, I N, Elliott, C M, Collard, J G, Exton, J H. Lysophosphatidic acid induces threonine phosphorylation of Tiam1 in Swiss 3T3 fibroblasts via activation of protein kinase C. J Biol Chem, 272(52), 33105-10, 1997.

Fleming, I.N., Elliott, C.M., Collard, J.G. and Exton, J.H. Lysophosphatidic acid induces phosphorylation of Tiam1 in Swiss 3T3 fibroblasts via activation of protein kinase C. J. Biol. Chem, 272, 33105-33110, 1997.

Guillemain, I, Exton, J H. Effects of brefeldin A on phosphatidylcholine phospholipase D and inositolphospholipid metabolism in HL-60 cells. Eur J Biochem, 249(3), 812-9, 1997.

Hess, J A, Ross, A H, Qiu, R G, Symons, M, Exton, J H. Role of Rho family proteins in phospholipase D activation by growth factors. J Biol Chem, 272(3), 1615-20, 1997.

Hess, J.A., Ross, A.H., Qui, R.-G., Symons, M. and Exton, J.H. Role of Rho family proteins in phospholipase D activation by growth factors. J. Biol. Chem., 272, 1615-1620, 1997.

Kanoh, H, Williger, B T, Exton, J H. Arfaptin 1, a putative cytosolic target protein of ADP-ribosylation factor, is recruited to Golgi membranes. J Biol Chem, 272(9), 5421-9, 1997.

Kanoh, H. and Exton, J.H. Arfaptin 1, a putative cytosolic target protein of ADP-ribosylation factor, is recruited to Golgi membranes. J. Biol. Chem., 272, 5421-5429, 1997.

Kanoh, H. and Exton, J.H. Arfaptin 1, a putative cytosolic target protein of ADP-ribosylation factor, is recruited to Golgi membranes. 272, 5421-5429, J. Biol. Chem, 1997.

Park, S K, Provost, J J, Bae, C D, Ho, W T, Exton, J H. Cloning and characterization of phospholipase D from rat brain. J Biol Chem, 272(46), 29263-71, 1997.

Park, S.-K., Provost, J.J., Bae, C.D., Ho, W.-T. and Exton, J.H. Cloning and characterization of phospholipase D from rat brain. J. Biol. Chem, 272, 29263-29271, 1997.

Yeo, E J, Provost, J J, Exton, J H. Dissociation of tyrosine phosphorylation and activation of phosphoinositide phospholipase C induced by the protein kinase C inhibitor Ro-31-8220 in Swiss 3T3 cells treated with platelet-derived growth factor. Biochim Biophys Acta, 1356(3), 308-20, 1997.

Fleming, I N, Elliott, C M, Exton, J H. Differential translocation of rho family GTPases by lysophosphatidic acid, endothelin-1, and platelet-derived growth factor. J Biol Chem, 271(51), 33067-73, 1996.

Fleming, I.N. and Exton, J.H. Differential translocation of Rho-family GTPases by lysophosphatidic acid, endothelin-1 and platelet-derived growth factor. J. Biol. Chem., 271, 33067-33073, 1996.

Malcolm, K C, Elliott, C M, Exton, J H. Evidence for Rho-mediated agonist stimulation of phospholipase D in rat1 fibroblasts. Effects of Clostridium botulinum C3 exoenzyme. J Biol Chem, 271(22), 13135-9, 1996.

Malcolm, K C, Sable, C L, Elliott, C M, Exton, J H. Enhanced phospholipase D activity and altered morphology in RhoA-overexpressing RAT1 fibroblasts. Biochem Biophys Res Commun, 225(2), 514-9, 1996.

Malcolm, K.C., Elliott, C.M. and Exton, J.H. Evidence for Rho-mediated agonist stimulation of phospholipase D in Rat1 fibroblasts. Effects of Clostridium botulinum C3 exoenzyme. J. Biol. Chem, 271, 13135-13139, 1996.

Pete, M J, Exton, J H. Purification of a lysophospholipase from bovine brain that selectively deacylates arachidonoyl-substituted lysophosphatidylcholine. J Biol Chem, 271(30), 18114-21, 1996.

Pete, M J, Wu, D W, Exton, J H. Subcellular fractions of bovine brain degrade phosphatidylcholine by sequential deacylation of the sn-1 and sn-2 positions. Biochim Biophys Acta, 1299(3), 325-32, 1996.

Provost, J J, Fudge, J, Israelit, S, Siddiqi, A R, Exton, J H. Tissue-specific distribution and subcellular distribution of phospholipase D in rat: evidence for distinct RhoA- and ADP-ribosylation factor (ARF)-regulated isoenzymes. Biochem J, 319 ( Pt 1), 285-91, 1996. PMCID:1217766

Venkatakrishnan, G, Exton, J H. Identification of determinants in the alpha-subunit of Gq required for phospholipase C activation. J Biol Chem, 271(9), 5066-72, 1996.

Venkatakrishnan, G. and Exton, J.H. Identification of determinants in the alpha subunit of G(q) required for phospholipase C activation. J. Biol. Chem., 271, 5066-5072, 1996.

Malcolm, K C, Trammell, S E, Exton, J H. Purinergic agonist and G protein stimulation of phospholipase D in rat liver plasma membranes. Independence from phospholipase C activation. Biochim Biophys Acta, 1268(2), 152-8, 1995.

Pete, M J, Exton, J H. Phospholipid interactions affect substrate hydrolysis by bovine brain phospholipase A1. Biochim Biophys Acta, 1256(3), 367-73, 1995.

Siddiqi, A R, Smith, J L, Ross, A H, Qiu, R G, Symons, M, Exton, J H. Regulation of phospholipase D in HL60 cells. Evidence for a cytosolic phospholipase D. J Biol Chem, 270(15), 8466-73, 1995.

Siddiqi, A.R., Smith, J.L., Ross, A.H., Qiu, R.-G., Symons, M. and Exton, J.H. Regulation of phospholipase D in HL60 cells. Evidence for a cytosolic phospholipase D. J. Biol. Chem, 270, 8466-8473, 1995.

Conricode, K M, Smith, J L, Burns, D J, Exton, J H. Phospholipase D activation in fibroblast membranes by the alpha and beta isoforms of protein kinase C. FEBS Lett, 342(2), 149-53, 1994.

Exton, J H. Messenger molecules derived from membrane lipids. Curr Opin Cell Biol, 6(2), 226-9, 1994.

Exton, J H. Phosphoinositide phospholipases and G proteins in hormone action. Annu Rev Physiol, 56, 349-69, 1994.

Malcolm, K.C., Ross, A.H., Qiu, R.-G., Symons, M. and Exton, J.H. Activation of rat liver phospholipase D by the small GTP- binding protein rhoA. J. Biol. Chem., 269, 25951-25954, 1994.

Pete, M J, Ross, A H, Exton, J H. Purification and properties of phospholipase A1 from bovine brain. J Biol Chem, 269(30), 19494-500, 1994.

Pete, M.J., Ross, A.H. and Exton, J.H. Purification and properties of phospholipase A1 from bovine brain. J. Biol. Chem, 269, 19494-19500, 1994.

Yeo, E J, Kazlauskas, A, Exton, J H. Activation of phospholipase C-gamma is necessary for stimulation of phospholipase D by platelet-derived growth factor. J Biol Chem, 269(45), 27823-6, 1994.

Yeo, E.-J., Kazlauskas, A. and Exton, J.H. Activation of phospholipase C-gamma is necessary for stimulation of phospholipase D by platelet-derived growth factor. 27823-27826, 269, J. Biol. Chem., 1994.

Allan, C J, Exton, J H. Quantification of inositol phospholipid breakdown in isolated rat hepatocytes. Biochem J, 290 ( Pt 3), 865-72, 1993. PMCID:1132361

Blank, J L, Shaw, K, Ross, A H, Exton, J H. Purification of a 110-kDa phosphoinositide phospholipase C that is activated by G-protein beta gamma-subunits. J Biol Chem, 268(33), 25184-91, 1993.

Exton, J H. Role of G proteins in activation of phosphoinositide phospholipase C. Adv Second Messenger Phosphoprotein Res, 28, 65-72, 1993.

Ha, K S, Exton, J H. Activation of actin polymerization by phosphatidic acid derived from phosphatidylcholine in IIC9 fibroblasts. J Cell Biol, 123(6 Pt 2), 1789-96, 1993. PMCID:2290890

Ha, K.-S. and Exton, J.H. Differential translocation of protein kinase C isozymes by thrombin and platelet-derived growth factor. J. Biol. Chem, 268, 10534-10539, 1993.

Nakanishi, H, Brewer, K A, Exton, J H. Activation of the zeta isozyme of protein kinase C by phosphatidylinositol 3,4,5-trisphosphate. J Biol Chem, 268(1), 13-6, 1993.

Berstein, G., Blank, J.L., Exton, J.H. and Ross, E.M. Reconstitution of agonist-stimulated phosphatidylinositol 4,5-bisphosphate hydrolysis using purified m1 muscarinic receptor, Gq/11 and phospholipase C-beta 1. J. Biol. Chem, 267, 8081-8088, 1992.

Berstein, G., Blank, J.L., Jhon, D.-Y., Exton, J.H., Rhee, S.G. and Ross, E.M. Phospholipase C-1 is a GTPase activating protein (GAP) for Gq/11, its physiologic regulator. Cell, 70, 411-418, 1992.

Blank, J L, Brattain, K A, Exton, J H. Activation of cytosolic phosphoinositide phospholipase C by G-protein beta gamma subunits. J Biol Chem, 267(32), 23069-75, 1992.

Blank, J.L., Brattain, K.A. and Exton, J.H. Activation of cytosolic phosphoinositide phospholipase C by G-protein beta gamma subunits. J. Biol. Chem, 267, 23069-23075, 1992.

Conricode, K.M. and Exton, J.H. Activation of phospholipase D by protein kinase C. Evidence for a phosphorylation independent mechanism. J. Biol. Chem , 267, 7199-7202, 1992.

Nakanishi, H. and Exton, J.H. Purification and characterization of the zeta isoform of protein kinase C from bovine kidney. J. Biol. Chem, 267, 16347-16354, 1992.

Shaw, K, Exton, J H. Identification in bovine liver plasma membranes of a Gq-activatable phosphoinositide phospholipase C. Biochemistry, 31(27), 6347-54, 1992.

Siddiqui, R A, Exton, J H. Phospholipid base exchange activity in rat liver plasma membranes. Evidence for regulation by G-protein and P2y-purinergic receptor. J Biol Chem, 267(9), 5755-61, 1992.

Siddiqui, R A, Exton, J H. Oleate stimulation of diacylglycerol formation from phosphatidylcholine through effects on phospholipase D and phosphatidate phosphohydrolase. Eur J Biochem, 210(2), 601-7, 1992.

Blank, J L, Ross, A H, Exton, J H. Purification and characterization of two G-proteins that activate the beta 1 isozyme of phosphoinositide-specific phospholipase C. Identification as members of the Gq class. J Biol Chem, 266(27), 18206-16, 1991.

Blank, J.L., Ross, A.H. and Exton, J.H. Purification and characterization of two G-proteins which activate the beta 1 isozyme of phosphoinositide-specific phospholipase C. Identification as members of the G(q) class. J. Biol. Chem., 266, 17206-18216, 1991.

Bocckino, S B, Wilson, P B, Exton, J H. Phosphatidate-dependent protein phosphorylation. Proc Natl Acad Sci U S A, 88(14), 6210-3, 1991. PMCID:52052

Exton, J H. Some thoughts on the mechanism of action of insulin. Diabetes, 40(5), 521-6, 1991.

Exton, J H, Taylor, S J, Augert, G, Bocckino, S B. Cell signalling through phospholipid breakdown. Mol Cell Biochem, 104(1-2), 81-6, 1991.

Stewart, S J, Cunningham, G R, Strupp, J A, House, F S, Kelley, L L, Henderson, G S, Exton, J H, Bocckino, S B. Activation of phospholipase D: a signaling system set in motion by perturbation of the T lymphocyte antigen receptor/CD3 complex. Cell Regul, 2(10), 841-50, 1991. PMCID:361879

Taylor, S J, Chae, H Z, Rhee, S G, Exton, J H. Activation of the beta 1 isozyme of phospholipase C by alpha subunits of the Gq class of G proteins. Nature, 350(6318), 516-8, 1991.

Taylor, S.J., Chae, H.Z., Rhee, S.G. and Exton, J.H. Activation of the Beta 1 isozyme of phospholipase C by purified a subunits of the G(q) class of G proteins. Nature, 350, 516-518, 1991.

Wange, R L, Smrcka, A V, Sternweis, P C, Exton, J H. Photoaffinity labeling of two rat liver plasma membrane proteins with [32P]gamma-azidoanilido GTP in response to vasopressin. Immunologic identification as alpha subunits of the Gq class of G proteins. J Biol Chem, 266(18), 11409-12, 1991.

Bouscarel, B, Augert, G, Taylor, S J, Exton, J H. Alterations in vasopressin and angiotensin II receptors and responses during culture of rat liver cells. Biochim Biophys Acta, 1055(3), 265-72, 1990.

Exton, J H. Signaling through phosphatidylcholine breakdown. J Biol Chem, 265(1), 1-4, 1990.

Exton, J H. Hormonal regulation of phosphatidylcholine breakdown. Adv Second Messenger Phosphoprotein Res, 24, 152-7, 1990.

Georgoussi, Z, Taylor, S J, Bocckino, S B, Exton, J H. Purification of the hepatic vasopressin receptor using a novel affinity column. Biochim Biophys Acta, 1055(1), 69-74, 1990.

Taylor, S J, Smith, J A, Exton, J H. Purification from bovine liver membranes of a guanine nucleotide-dependent activator of phosphoinositide-specific phospholipase C. Immunologic identification as a novel G-protein alpha subunit. J Biol Chem, 265(28), 17150-6, 1990.

Augert, G, Blackmore, P F, Exton, J H. Changes in the concentration and fatty acid composition of phosphoinositides induced by hormones in hepatocytes. J Biol Chem, 264(5), 2574-80, 1989.

Augert, G, Bocckino, S B, Blackmore, P F, Exton, J H. Hormonal stimulation of diacylglycerol formation in hepatocytes. Evidence for phosphatidylcholine breakdown. J Biol Chem, 264(36), 21689-98, 1989.

Augert, G., Blackmore, P.F. and Exton, J.H. Changes in the concentration and fatty acid composition of phosphoinositides induced by hormones in hepatocytes. J. Biol. Chem., 264, 2574-2580, 1989.

Augert, G., Bocckino, S.B., Blackmore, P.F.and Exton, J.H. Hormonal stimulation of diacylglycerol formation in hepatocytes. Evidence for phosphatidylcholine breakdown. J. Biol. Chem., 264, 21689-21698, 1989.

Bocckino, S B, Wilson, P B, Exton, J H. An early elevation of diacylglycerol and phosphatidate in regenerating liver. Biochem Biophys Res Commun, 164(1), 290-4, 1989.

Lynch, C J, Blackmore, P F, Johnson, E H, Wange, R L, Krone, P K, Exton, J H. Guanine nucleotide binding regulatory proteins and adenylate cyclase in livers of streptozotocin- and BB/Wor-diabetic rats. Immunodetection of Gs and Gi with antisera prepared against synthetic peptides. J Clin Invest, 83(6), 2050-62, 1989. PMCID:303930

Stewart, S J, Prpic, V, Johns, J A, Powers, F S, Graber, S E, Forbes, J T, Exton, J H. Bacterial toxins affect early events of T lymphocyte activation. J Clin Invest, 83(1), 234-42, 1989. PMCID:303667

Augert, G, Exton, J H. Insulin and oxytocin effects on phosphoinositide metabolism in adipocytes. J Biol Chem, 263(8), 3600-9, 1988.

Blackmore, P F, Lynch, C J, Uhing, R J, Fitzgerald, T, Bocckino, S B, Exton, J H. Role of guanine nucleotide regulatory proteins and inositol phosphates in the hormone induced mobilization of hepatocyte calcium. Adv Exp Med Biol, 232, 169-82, 1988.

Bouscarel, B, Blackmore, P F, Exton, J H. Characterization of the angiotensin II receptor in primary cultures of rat hepatocytes. Evidence that a single population is coupled to two different responses. J Biol Chem, 263(29), 14913-9, 1988.

Bouscarel, B, Meurer, K, Decker, C, Exton, J H. The role of protein kinase C in the inactivation of hepatic glycogen synthase by calcium-mobilizing agonists. Biochem J, 251(1), 47-53, 1988. PMCID:1148962

Bouscarel, B, Wilson, P B, Blackmore, P F, Lynch, C J, Exton, J H. Agonist-induced down-regulation of the angiotensin II receptor in primary cultures of rat hepatocytes. J Biol Chem, 263(29), 14920-4, 1988.

Exton, J H. Role of phosphoinositides in the regulation of liver function. Hepatology, 8(1), 152-66, 1988.

Exton, J H. Mechanisms of action of calcium-mobilizing agonists: some variations on a young theme. FASEB J, 2(11), 2670-6, 1988.

Lynch, C J, Taylor, S J, Smith, J A, Exton, J H. Formation of the high-affinity agonist state of the alpha 1-adrenergic receptor at cold temperatures does not require a G-protein. FEBS Lett, 229(1), 54-8, 1988.

Bocckino, S B, Wilson, P B, Exton, J H. Ca2+-mobilizing hormones elicit phosphatidylethanol accumulation via phospholipase D activation. FEBS Lett, 225(1-2), 201-4, 1987.

Bocckino, S.B., Blackmore, P.F., Wilson, P.B., and Exton, J.H. Phosphatidate accumulation in hormone-treated hepatocytes via a phospholipase D mechanism. J. Biol. Chem., 262, 15309-15315, 1987.

Exton, J H. Calcium signalling in cells--molecular mechanisms. Kidney Int Suppl, 23, S68-81, 1987.

Irving, H. and Exton, J.H. Phosphatidylcholine breakdown in rat liver plasma membranes. J.Biol.Chem., 262, 3440-3443, 1987.

Lynch, C J, Bocckino, S B, Blackmore, P F, Exton, J H. Calcium-mobilizing hormones and phorbol myristate acetate mediate heterologous desensitization of the hormone-sensitive hepatic Na+/K+ pump. Biochem J, 248(3), 807-13, 1987. PMCID:1148621

Taylor, S J, Exton, J H. Guanine-nucleotide and hormone regulation of polyphosphoinositide phospholipase C activity of rat liver plasma membranes. Bivalent-cation and phospholipid requirements. Biochem J, 248(3), 791-9, 1987. PMCID:1148619


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Postdoctoral Position Details
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Updated Date
04/25/2007



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