Vanderbilt University School of Medicine

Matusik, Robert J. , Ph.D.
Professor of Urologic Surgery
Professor of Cancer Biology
William L. Bray Chair in Urology
Professor of Cell and Developmental Biology

Lab Url:

Phone Number: 615-322-2142 or 615-343-1902


Matusik, Robert's picture

Office Address   Mailing Address

A-1302 Medical Center North

A-1302 Medical Center North

Research Keywords
Cancer,Developmental biology,Endocrinology,Gene regulation,Gene therapy,Proteomics,Receptor,Recombination,Transcription, Transcription factor,Transformation,Chromatin,Endocrinology,Fertility,Gene regulation,Gene therapy,Genetics,Genome,Genomics,Mouse,Receptor,Reproduction,Transcription factor

Research Specialty
Androgen Regulation of Gene Expression, Transgenic Mouse Models for Prostate Cancer, and Gene Therapy

Research Description
In North America, one in ten men is diagnosed with prostate cancer. Prostate cancer starts as an androgen dependent disease that progresses to an androgen independent cancer. The androgen independent tumors fail therapy. One of the major impediments to prostate cancer research is that no appropriate animal model system adequately displays the recognized stages of human prostatic disease. Further, no new therapeutic approaches have been developed after current therapy fails. Our laboratory has developed the probasin (PB) gene as a model system for androgen action, to establish new transgenic animal models for prostate cancer, and to test new therapeutic approaches for treatment.

The androgen receptor (AR) can function through similar cis-acting DNA elements similar to the palindromic glucocorticoid response element; however, recent data has shown that distinct AR elements (ARE) exist and function via cooperative interactions of multiple elements in the PB promoter. Further, since the PB promoter directs prostate specific expression in transgenic mice, it has become a model to dissect the key cis-acting DNA elements that control prostate-specific gene expression. Our laboratory has designed new PB promoters that target high levels of transgene expression to the prostate. These new promoters now serve to create new animal models for prostate cancer and to develop gene therapy vectors that will target a therapeutic gene(s) in the treatment of prostate cancer.

The PB promoter has been linked to oncogenes and growth factors to create new transgenic mouse models for prostate cancer. Transgenic mice carrying PB-oncogenes develop various stages of disease including prostatic precursor lesions that advance to an adenocarcinoma and then to high grade metastatic cancer. Gene expression profiles are being analyzed in these animal models to identify the key genes involved in tumor progression. By identifying the genes that are responsible for tumor progression, we can develop new targets for therapeutic intervention. .

Clayton, DB, Stephany, HA, Ching, CB, Rahman, SA, Tanaka, ST, Thomas, JC, Pope, JC, Adams, MC, Brock, JW, Clark, PE, Hayward, SW, Matusik, RJ, Milne, GL. F2-isoprostanes as a biomarker of oxidative stress in the mouse bladder. J Urol, 191(5 Suppl), 1597-601, 2014.

DeGraff, DJ, Grabowska, MM, Case, TC, Yu, X, Herrick, MK, Hayward, WJ, Strand, DW, Cates, JM, Hayward, SW, Gao, N, Walter, MA, Buttyan, R, Yi, Y, Kaestner, KH, Matusik, RJ. FOXA1 deletion in luminal epithelium causes prostatic hyperplasia and alteration of differentiated phenotype. Lab Invest, 94(7), 726-39, 2014.

Grabowska, MM, Degraff, DJ, Yu, X, Jin, RJ, Chen, Z, Borowsky, AD, Matusik, RJ. Mouse models of prostate cancer: picking the best model for the question. Cancer Metastasis Rev, , , 2014.

Grabowska, MM, Elliott, AD, DeGraff, DJ, Anderson, PD, Anumanthan, G, Yamashita, H, Sun, Q, Friedman, DB, Hachey, DL, Yu, X, Sheehan, JH, Ahn, JM, Raj, GV, Piston, DW, Gronostajski, RM, Matusik, RJ. NFI transcription factors interact with FOXA1 to regulate prostate-specific gene expression. Mol Endocrinol, 28(6), 949-64, 2014.

Jin, R, Yi, Y, Yull, FE, Blackwell, TS, Clark, PE, Koyama, T, Smith, JA, Matusik, RJ. NF-??B gene signature predicts prostate cancer progression. Cancer Res, 74(10), 2763-72, 2014.

Xie, Y, Liu, S, Lu, W, Yang, Q, Williams, KD, Binhazim, AA, Carver, BS, Matusik, RJ, Chen, Z. Slug regulates E-cadherin repression via p19Arf in prostate tumorigenesis. Mol Oncol, , , 2014.

DeGraff, DJ, Cates, JM, Mauney, JR, Clark, PE, Matusik, RJ, Adam, RM. When urothelial differentiation pathways go wrong: implications for bladder cancer development and progression. Urol Oncol, 31(6), 802-11, 2013.

Jin, R, Sterling, JA, Edwards, JR, DeGraff, DJ, Lee, C, Park, SI, Matusik, RJ. Activation of NF-kappa B signaling promotes growth of prostate cancer cells in bone. PLoS One, 8(4), e60983, 2013.

Stephany, HA, Strand, DW, Ching, CB, Tanaka, ST, Milne, GL, Cajaiba, MM, Thomas, JC, Pope, JC, Adams, MC, Brock, JW, Hayward, SW, Matusik, RJ, Clayton, DB. Chronic cyclic bladder over distention up-regulates hypoxia dependent pathways. J Urol, 190(4 Suppl), 1603-9, 2013.

Strand, DW, DeGraff, DJ, Jiang, M, Sameni, M, Franco, OE, Love, HD, Hayward, WJ, Lin-Tsai, O, Wang, AY, Cates, JM, Sloane, BF, Matusik, RJ, Hayward, SW. Deficiency in metabolic regulators PPAR?? and PTEN cooperates to drive keratinizing squamous metaplasia in novel models of human tissue regeneration. Am J Pathol, 182(2), 449-59, 2013.

Xiang, Y, Qiu, Q, Jiang, M, Jin, R, Lehmann, BD, Strand, DW, Jovanovic, B, DeGraff, DJ, Zheng, Y, Yousif, DA, Simmons, CQ, Case, TC, Yi, J, Cates, JM, Virostko, J, He, X, Jin, X, Hayward, SW, Matusik, RJ, George, AL, Yi, Y. SPARCL1 suppresses metastasis in prostate cancer. Mol Oncol, 7(6), 1019-30, 2013.

DeGraff, DJ, Clark, PE, Cates, JM, Yamashita, H, Robinson, VL, Yu, X, Smolkin, ME, Chang, SS, Cookson, MS, Herrick, MK, Shariat, SF, Steinberg, GD, Frierson, HF, Wu, XR, Theodorescu, D, Matusik, RJ. Loss of the urothelial differentiation marker FOXA1 is associated with high grade, late stage bladder cancer and increased tumor proliferation. PLoS One, 7(5), e36669, 2012.

Drzewiecki, BA, Anumanthan, G, Penn, HA, Tanaka, ST, Thomas, JC, Adams, MC, Brock, JW, Pope, JC, Matusik, RJ, Hayward, S, Clayton, DB. Modulation of the hypoxic response following partial bladder outlet obstruction. J Urol, 188(4 Suppl), 1549-54, 2012.

Yu, X, Wang, Y, DeGraff, DJ, Wills, ML, Matusik, RJ. Wnt/I?-catenin activation promotes prostate tumor progression in a mouse model. Oncogene, 30(16), 1868-79, 2011.

Jiang, M, Fernandez, S, Jerome, WG, He, Y, Yu, X, Cai, H, Boone, B, Yi, Y, Magnuson, MA, Roy-Burman, P, Matusik, RJ, Shappell, SB, Hayward, SW. Disruption of PPARgamma signaling results in mouse prostatic intraepithelial neoplasia involving active autophagy. Cell Death Differ, 17(3), 469-81, 2010. PMCID:2821953

Nandana, S, Ellwood-Yen, K, Sawyers, C, Wills, M, Weidow, B, Case, T, Vasioukhin, V, Matusik, R. Hepsin cooperates with MYC in the progression of adenocarcinoma in a prostate cancer mouse model. Prostate, 70(6), 591-600, 2010. PMCID:2925264

Zhang, J, Gao, N, DeGraff, DJ, Yu, X, Sun, Q, Case, TC, Kasper, S, Matusik, RJ. Characterization of cis elements of the probasin promoter necessary for prostate-specific gene expression. Prostate, 70(9), 934-51, 2010.

Anumanthan, G, Tanaka, ST, Adams, CM, Thomas, JC, Wills, ML, Adams, MC, Hayward, SW, Matusik, RJ, Bhowmick, NA, Brock, JW, Pope, JC. Bladder stromal loss of transforming growth factor receptor II decreases fibrosis after bladder obstruction. J Urol, 182(4 Suppl), 1775-80, 2009. PMCID:2821953

Li, X, Wang, Y, Sharif-Afshar, AR, Uwamariya, C, Yi, A, Ishii, K, Hayward, SW, Matusik, RJ, Bhowmick, NA. Urothelial transdifferentiation to prostate epithelia is mediated by paracrine TGF-beta signaling. Differentiation, 77(1), 95-102, 2009. PMCID:2733217

Sun, Q, Yu, X, Degraff, DJ, Matusik, RJ. Upstream stimulatory factor 2, a novel FoxA1-interacting protein, is involved in prostate-specific gene expression. Mol Endocrinol, 23(12), 2038-47, 2009. PMCID:2796152

Yu, X, Wang, Y, Jiang, M, Bierie, B, Roy-Burman, P, Shen, MM, Taketo, MM, Wills, M, Matusik, RJ. Activation of beta-Catenin in mouse prostate causes HGPIN and continuous prostate growth after castration. Prostate, 69(3), 249-62, 2009.

Anumanthan, G, Makari, JH, Honea, L, Thomas, JC, Wills, ML, Bhowmick, NA, Adams, MC, Hayward, SW, Matusik, RJ, Brock, JW, Pope, JC. Directed differentiation of bone marrow derived mesenchymal stem cells into bladder urothelium. J Urol, 180(4 Suppl), 1778-83, 2008.

Gupta, A, Wang, Y, Browne, C, Kim, S, Case, T, Paul, M, Wills, ML, Matusik, RJ. Neuroendocrine differentiation in the 12T-10 transgenic prostate mouse model mimics endocrine differentiation of pancreatic beta cells. Prostate, 68(1), 50-60, 2008.

Jin, RJ, Lho, Y, Connelly, L, Wang, Y, Yu, X, Saint Jean, L, Case, TC, Ellwood-Yen, K, Sawyers, CL, Bhowmick, NA, Blackwell, TS, Yull, FE, Matusik, RJ. The nuclear factor-kappaB pathway controls the progression of prostate cancer to androgen-independent growth. Cancer Res, 68(16), 6762-9, 2008.

Jin, RJ, Lho, Y, Wang, Y, Ao, M, Revelo, MP, Hayward, SW, Wills, ML, Logan, SK, Zhang, P, Matusik, RJ. Down-regulation of p57Kip2 induces prostate cancer in the mouse. Cancer Res, 68(10), 3601-8, 2008.

Martinez-Ferrer, M, Iturregui, JM, Uwamariya, C, Starkman, J, Sharif-Afshar, AR, Suzuki, K, Visedsindh, W, Matusik, RJ, Dmochowski, RR, Bhowmick, NA. Role of nicotinic and estrogen signaling during experimental acute and chronic bladder inflammation. Am J Pathol, 172(1), 59-67, 2008. PMCID:2189612

Matusik, RJ, Jin, RJ, Sun, Q, Wang, Y, Yu, X, Gupta, A, Nandana, S, Case, TC, Paul, M, Mirosevich, J, Oottamasathien, S, Thomas, J. Prostate epithelial cell fate. Differentiation, , , 2008.

Placencio, VR, Sharif-Afshar, AR, Li, X, Huang, H, Uwamariya, C, Neilson, EG, Shen, MM, Matusik, RJ, Hayward, SW, Bhowmick, NA. Stromal transforming growth factor-beta signaling mediates prostatic response to androgen ablation by paracrine Wnt activity. Cancer Res, 68(12), 4709-18, 2008.

Thomas, JC, Oottamasathien, S, Makari, JH, Honea, L, Sharif-Afshar, AR, Wang, Y, Adams, C, Wills, ML, Bhowmick, NA, Adams, MC, Brock, JW, Hayward, SW, Matusik, RJ, Pope, JC. Temporal-spatial protein expression in bladder tissue derived from embryonic stem cells. J Urol, 180(4 Suppl), 1784-9, 2008.

Oottamasathien, S, Wang, Y, Williams, K, Franco, OE, Wills, ML, Thomas, JC, Saba, K, Sharif-Afshar, AR, Makari, JH, Bhowmick, NA, Demarco, RT, Hipkens, S, Magnuson, M, Brock, JW, Hayward, SW, Pope, JC, Matusik, RJ. Directed differentiation of embryonic stem cells into bladder tissue. Dev Biol, 304(2), 556-566, 2007. PMCID:1994155

Suzuki, K, Yu, X, Chaurand, P, Araki, Y, Lareyre, JJ, Caprioli, RM, Orgebin-Crist, MC, Matusik, RJ. Epididymis-specific lipocalin promoters. Asian J Androl, 9(4), 515-21, 2007.

Lees, JR, Charbonneau, B, Hayball, JD, Diener, K, Brown, M, Matusik, R, Cohen, MB, Ratliff, TL. T-cell recognition of a prostate specific antigen is not sufficient to induce prostate tissue destruction. Prostate, 66(6), 578-90, 2006.

Lees, JR, Charbonneau, B, Swanson, AK, Jensen, R, Zhang, J, Matusik, R, Ratliff, TL. Deletion is neither sufficient nor necessary for the induction of peripheral tolerance in mature CD8+ T cells. Immunology, 117(2), 248-61, 2006. PMCID:1782220

Mirosevich, J, Gao, N, Gupta, A, Shappell, SB, Jove, R, Matusik, RJ. Expression and role of Foxa proteins in prostate cancer. Prostate, 66(10), 1013-1028, 2006.

Shao, TC, Li, HL, Kasper, S, Matusik, R, Ittmann, M, Cunningham, GR. Comparison of the growth-promoting effects of testosterone and 7-alpha-methyl-19-nor-testosterone (MENT) on the prostate and levator ani muscle of LPB-tag transgenic mice. Prostate, 66(4), 369-76, 2006.

Suzuki, K, Yu, X, Chaurand, P, Araki, Y, Lareyre, JJ, Caprioli, RM, Matusik, RJ, Orgebin-Crist, MC. Epididymis-specific promoter-driven gene targeting: A transcription factor which regulates epididymis-specific gene expression. Mol Cell Endocrinol, 250, 184-189, 2006.

Uchida, K, Masumori, N, Takahashi, A, Itoh, N, Kato, K, Matusik, RJ, Tsukamoto, T. Murine androgen-independent neuroendocrine carcinoma promotes metastasis of human prostate cancer cell line LNCaP. Prostate, 66(5), 536-45, 2006.

Wang, Y, Kasper, S, Yuan, J, Jin, RJ, Zhang, J, Ishii, K, Wills, ML, Hayward, SW, Matusik, RJ. Androgen-dependent prostate epithelial cell selection by targeting ARR(2)PBneo to the LPB-Tag model of prostate cancer. Lab Invest, 86(10), 1074-88, 2006.

Yu, X, Suzuki, K, Wang, Y, Gupta, A, Jin, R, Orgebin-Crist, MC, Matusik, R. The Role of Forkhead Box A2 (Foxa2) to Restrict Androgen Regulated Gene Expression of Lipocalin 5 in the Mouse Epididymis. Mol Endocrinol, 10, 2418-2431, 2006.

Best, CJ, Gillespie, JW, Yi, Y, Chandramouli, GV, Perlmutter, MA, Gathright, Y, Erickson, HS, Georgevich, L, Tangrea, MA, Duray, PH, Gonz?!lez, S, Velasco, A, Linehan, WM, Matusik, RJ, Price, DK, Figg, WD, Emmert-Buck, MR, Chuaqui, RF. Molecular alterations in primary prostate cancer after androgen ablation therapy. Clin Cancer Res, 11(19 Pt 1), 6823-34, 2005. PMCID:1432092

Gao, N, Ishii, K, Mirosevich, J, Kuwajima, S, Oppenheimer, SR, Roberts, RL, Jiang, M, Yu, X, Shappell, SB, Caprioli, RM, Stoffel, M, Hayward, SW, Matusik, RJ. Forkhead box A1 regulates prostate ductal morphogenesis and promotes epithelial cell maturation. Development, 132(15), 3431-43, 2005.

Ishii, K, Shappell, SB, Matusik, RJ, Hayward, SW. Use of tissue recombination to predict phenotypes of transgenic mouse models of prostate carcinoma. Lab Invest, 85, 1086-1103, 2005.

Mirosevich, J, Gao, N, Matusik, RJ. Expression of Foxa transcription factors in the developing and adult murine prostate. Prostate, 62(4), 339-52, 2005.

Yi, Y, Mirosevich, J, Shyr, Y, Matusik, R, George, AL. Coupled analysis of gene expression and chromosomal location. Genomics, 85(3), 401-12, 2005.

Yu, X, Gupta, A, Wang, Y, Suzuki, K, Mirosevich, J, Orgebin-Crist, MC, Matusik, RJ. Foxa1 and foxa2 interact with the androgen receptor to regulate prostate and epididymal genes differentially. Ann N Y Acad Sci, 1061, 77-93, 2005.

Jin, RJ, Wang, Y, Masumori, N, Ishii, K, Tsukamoto, T, Shappell, SB, Hayward, SW, Kasper, S, Matusik, RJ. NE-10 neuroendocrine cancer promotes the LNCaP xenograft growth in castrated mice. Cancer Res, 64(15), 5489-95, 2004.

Kirchhoff, Christiane, Araki, Yoshihiko, Huhtaniemi, Ilpo, Matusik, Robert J, Osterhoff, Caroline, Poutanen, Matti, Samalecos, Annemarie, Sipil??, Petra, Suzuki, Kichiya, Orgebin-Crist, Marie-Claire. Immortalization by large T-antigen of the adult epididymal duct epithelium. Mol Cell Endocrinol, 216(1-2), 83-94, 2004.

Klezovitch, O, Chevillet, J, Mirosevich, J, Roberts, RL, Matusik, RJ, Vasioukhin, V. Hepsin promotes prostate cancer progression and metastasis. Cancer Cell, 6(2), 185-95, 2004.

Masumori, Naoya, Tsuchiya, Karen, Tu, William H, Lee, Charles, Kasper, Susan, Tsukamoto, Taiji, Shappell, Scott B, Matusik, Robert J. An allograft model of androgen independent prostatic neuroendocrine carcinoma derived from a large probasin promoter-T antigen transgenic mouse line. J Urol, 171(1), 439-42, 2004.

Suzuki, K, Lareyre, JJ, S?!nchez, D, Gutierrez, G, Araki, Y, Matusik, RJ, Orgebin-Crist, MC. Molecular evolution of epididymal lipocalin genes localized on mouse chromosome 2. Gene, 339, 49-59, 2004.

Suzuki, Kichiya, Drevet, Joel, Hinton, Barry T, Huhtaniemi, Ilpo, Lareyre, Jean-Jacques, Matusik, Robert J, Pons, Eric, Poutanen, Matti, Sipil??, Petra, Orgebin-Crist, Marie-Claire. Epididymis-specific promoter-driven gene targeting: a new approach to control epididymal function. Mol Cell Endocrinol, 216(1-2), 15-22, 2004.

Zhang, JianFeng, Gao, Nan, Kasper, Susan, Reid, Kimberly, Nelson, Colleen, Matusik, Robert J. An androgen-dependent upstream enhancer is essential for high levels of probasin gene expression. Endocrinology, 145(1), 134-48, 2004.

Ellwood-Yen, K, Graeber, TG, Wongvipat, J, Iruela-Arispe, ML, Zhang, J, Matusik, R, Thomas, GV, Sawyers, CL. Myc-driven murine prostate cancer shares molecular features with human prostate tumors. Cancer Cell, 4(3), 223-38, 2003.

Fouch??court, Sophie, Lareyre, Jean-Jacques, Chaurand, Pierre, DaGue, Beverly B, Suzuki, Kichiya, Ong, David E, Olson, Gary E, Matusik, Robert J, Caprioli, Richard M, Orgebin-Crist, Marie-Claire. Identification, immunolocalization, regulation, and postnatal development of the lipocalin EP17 (epididymal protein of 17 kilodaltons) in the mouse and rat epididymis. Endocrinology, 144, 887-900, 2003.

Gao, Nan, Zhang, Jianfeng, Rao, Mira A., Case, Thomas C., Mirosevich, Janni, Wang, Yongqing, Jin, Renjie, Gupta, Aparna, Rennie, Paul S., Matusik, Robert J. The Role of Hepatocyte Nuclear Factor-3{alpha} (Foxa1) and Androgen Receptor in Transcriptional Regulation of Prostatic Genes. Mol Endocrinol, (17), 1484-1507, 2003.

Shappell SB., Masumori M, Thomas TZ, Case T, Paul M, Kasper S, Matusik RJ. . Transgenic mouse models of prostate carcinoma: Anatomic, Histopathologic, and molecular considerations. . In: Prostate Cancer: Scientific and Clinical Aspects of Bridging the Gap, ed. by PD Abel and E-N Lalani. Imperial College Press (London), , 245-319(Chapter 3), , 2003.

Shappell, Scott B, Olson, Sandra J, Hannah, S Erin, Manning, Suzanne, Roberts, Richard L, Masumori, Naoya, Jisaka, Mitsuo, Boeglin, William E, Vader, Virginia, Dave, Dhiren S, Shook, M Frances, Thomas, Tania Z, Funk, Colin D, Brash, Alan R, Matusik, Robert J. Elevated expression of 12/15-lipoxygenase and cyclooxygenase-2 in a transgenic mouse model of prostate carcinoma. Cancer Res, 63, 2256-67, 2003.

Suzuki, Kichiya, Araki, Yoshihiko, Zhu, Mei-Ying, Lareyre, Jean-Jacques, Matusik, Robert J, Orgebin-Crist, Marie-Claire. The 5'-flanking region of the murine epididymal protein of 17 kilodaltons gene targets transgene expression in the epididymis. Endocrinology, 144, 877-86, 2003.

Tu, William H, Thomas, Tania Z, Masumori, Naoya, Bhowmick, Neil A, Gorska, Agnieszka E, Shyr, Yu, Kasper, Susan, Case, Tom, Roberts, Richard L, Shappell, Scott B, Moses, Harold L, Matusik, Robert J. The loss of TGF-beta signaling promotes prostate cancer metastasis. Neoplasia, 5(3), 267-77, 2003. PMCID:1502411

Wen, Yong, Giri, Dipak, Yan, Duen-Hwa, Spohn, Bill, Zinner, Ralph G, Xia, Weiya, Thompson, Timothy C, Matusik, Robert J, Hung, Mien-Chie. Prostate-specific antitumor activity by probasin promoter-directed p202 expression. Mol Carcinog, 37(3), 130-7, 2003.

Matusik RJ, Masumori M, Thomas TZ, Case T, Paul M, Kasper S, Shappell SB. Transgenic mouse models of prostate cancer. In: Transgenics in Endocrinology, ed. by MM Matzuk, CW Brown, and TR Kumar. The Humana Press Inc (Totowa, NJ) pp 399-423, 2001

Araki, Yoshihiko, Suzuki, Kichiya, Matusik, Robert J, Obinata, Masuo, Orgebin-Crist, Marie-Claire. Immortalized epididymal cell lines from transgenic mice overexpressing temperature-sensitive simian virus 40 large T-antigen gene. J Androl, 23(6), 854-69, 2002.

Fouch??court, Sophie, Chaurand, Pierre, DaGue, Beverly B, Lareyre, Jean-Jacques, Matusik, Robert J, Caprioli, Richard M, Orgebin-Crist, Marie-Claire. Epididymal lipocalin-type prostaglandin D2 synthase: identification using mass spectrometry, messenger RNA localization, and immunodetection in mouse, rat, hamster, and monkey. Biol Reprod, 66(2), 524-33, 2002.

Logg, Christopher R, Logg, Aki, Matusik, Robert J, Bochner, Bernard H, Kasahara, Noriyuki. Tissue-specific transcriptional targeting of a replication-competent retroviral vector. J Virol, 76, 12783-91, 2002. PMCID:136666

Song, Zhigang, Wu, Xiantuo, Powell, William C, Cardiff, Robert D, Cohen, Michael B, Tin, Robert T, Matusik, Robert J, Miller, Gary J, Roy-Burman, Pradip. Fibroblast growth factor 8 isoform B overexpression in prostate epithelium: a new mouse model for prostatic intraepithelial neoplasia. Cancer Res, 62(17), 5096-105, 2002.

Andriani, F, Nan, B, Yu, J, Li, X, Weigel, N L, McPhaul, M J, Kasper, S, Kagawa, S, Fang, B, Matusik, R J, Denner, L, Marcelli, M. Use of the probasin promoter ARR2PB to express Bax in androgen receptor-positive prostate cancer cells. J Natl Cancer Inst, 93(17), 1314-24, 2001.

Buchanan, G, Yang, M, Harris, J M, Nahm, H S, Han, G, Moore, N, Bentel, J M, Matusik, R J, Horsfall, D J, Marshall, V R, Greenberg, N M, Tilley, W D. Mutations at the boundary of the hinge and ligand binding domain of the androgen receptor confer increased transactivation function. Mol Endocrinol, 15(1), 46-56, 2001.

Lareyre, J J, Winfrey, V P, Kasper, S, Ong, D E, Matusik, R J, Olson, G E, Orgebin-Crist, M C. Gene duplication gives rise to a new 17-kilodalton lipocalin that shows epididymal region-specific expression and testicular factor(s) regulation. Endocrinology, 142(3), 1296-308, 2001.

Masumori, N, Thomas, T Z, Chaurand, P, Case, T, Paul, M, Kasper, S, Caprioli, R M, Tsukamoto, T, Shappell, S B, Matusik, R J. A probasin-large T antigen transgenic mouse line develops prostate adenocarcinoma and neuroendocrine carcinoma with metastatic potential. Cancer Res, 61(5), 2239-49, 2001.

Shappell, S B, Gupta, R A, Manning, S, Whitehead, R, Boeglin, W E, Schneider, C, Case, T, Price, J, Jack, G S, Wheeler, T M, Matusik, R J, Brash, A R, Dubois, R N. 15S-Hydroxyeicosatetraenoic acid activates peroxisome proliferator-activated receptor gamma and inhibits proliferation in PC3 prostate carcinoma cells. Cancer Res, 61(2), 497-503, 2001.

Wu, X, Wu, J, Huang, J, Powell, W C, Zhang, J, Matusik, R J, Sangiorgi, F O, Maxson, R E, Sucov, H M, Roy-Burman, P. Generation of a prostate epithelial cell-specific Cre transgenic mouse model for tissue-specific gene ablation. Mech Dev, 101(1-2), 61-9, 2001.

Xie, X, Zhao, X, Liu, Y, Zhang, J, Matusik, R J, Slawin, K M, Spencer, D M. Adenovirus-mediated tissue-targeted expression of a caspase-9-based artificial death switch for the treatment of prostate cancer. Cancer Res, 61(18), 6795-804, 2001.

Kasper, S, Matusik, R J. Rat probasin: structure and function of an outlier lipocalin. Biochim Biophys Acta, 1482(1-2), 249-58, 2000.

Lareyre, J J, Reid, K, Nelson, C, Kasper, S, Rennie, P S, Orgebin-Crist, M C, Matusik, R J. Characterization of an androgen-specific response region within the 5' flanking region of the murine epididymal retinoic acid binding protein gene. Biol Reprod, 63(6), 1881-92, 2000.

Zhang, J, Thomas, T Z, Kasper, S, Matusik, R J. A small composite probasin promoter confers high levels of prostate-specific gene expression through regulation by androgens and glucocorticoids in vitro and in vivo. Endocrinology, 141(12), 4698-710, 2000.

Brash, AR, Jisaka, M, Boeglin, WE, Chang, MS, Keeney, DS, Nanney, LB, Kasper, S, Matusik, RJ, Olson, SJ, Shappell, SB. Investigation of a second 15S-lipoxygenase in humans and its expression in epithelial tissues. Adv Exp Med Biol, 469, 83-9, 1999.

Kasper, S, Rennie, P S, Bruchovsky, N, Lin, L, Cheng, H, Snoek, R, Dahlman-Wright, K, Gustafsson, J A, Shiu, R P, Sheppard, P C, Matusik, R J. Selective activation of the probasin androgen-responsive region by steroid hormones. J Mol Endocrinol, 22(3), 313-25, 1999.

Lareyre, J J, Thomas, T Z, Zheng, W L, Kasper, S, Ong, D E, Orgebin-Crist, M C, Matusik, R J. A 5-kilobase pair promoter fragment of the murine epididymal retinoic acid-binding protein gene drives the tissue-specific, cell-specific, and androgen-regulated expression of a foreign gene in the epididymis of transgenic mice. J Biol Chem, 274(12), 8282-90, 1999.

Bai, G, Kasper, S, Matusik, R J, Rennie, P S, Moshier, J A, Krongrad, A. Androgen regulation of the human ornithine decarboxylase promoter in prostate cancer cells. J Androl, 19(2), 127-35, 1998.

Chan, J, Song, C S, Matusik, R J, Chatterjee, B, Roy, A K. Inhibition of androgen action by dehydroepiandrosterone sulfotransferase transfected in PC-3 prostate cancer cells. Chem Biol Interact, 109(1-3), 267-78, 1998.

Green, JE, Greenberg, NM, Ashendel, CL, Barrett, JC, Boone, C, Getzenberg, RH, Henkin, J, Matusik, R, Janus, TJ, Scher, HI. Workgroup 3: transgenic and reconstitution models of prostate cancer. Prostate, 36(1), 59-63, 1998.

Kasper, S, Sheppard, P C, Yan, Y, Pettigrew, N, Borowsky, A D, Prins, G S, Dodd, J G, Duckworth, M L, Matusik, R J. Development, progression, and androgen-dependence of prostate tumors in probasin-large T antigen transgenic mice: a model for prostate cancer. Lab Invest, 78(3), 319-33, 1998.

Kasper, S, Sheppard, PC, Yan, Y, Pettigrew, N, Borowsky, AD, Prins, GS, Dodd, JG, Duckworth, ML, Matusik, RJ. Development, progression, and androgen-dependence of prostate tumors in probasin-large T antigen transgenic mice: a model for prostate cancer. Lab Invest, 78(6), i-xv, 1998.

Lareyre JJ, Mattei M-G, Kasper S, Newcomer ME, Ong DE, Matusik RJ and Orgebin- Crist M-C. Structure and putative function of a murine epididymal retinoic acid-binding protein (mE-RABP). J. of Reprod. and Fert. Supplement , 53, 59-65, 1998.

Lareyre JJ, Mattei M-G, Kasper S, Ong DE, Matusik RJ and Orgebin-Crist M-C. Genomic organization and chromosomal localization of the murine epididymal retinoic acid binding protein (mE-RABP) gene. Mol. Reprod. and Dev, 50, 387-395, 1998.

Lareyre JJ, Zheng WL, Zhao GQ, Kasper S, Newcomer ME, Matusik RJ, Ong DE and Orgebin-Crist MC. Molecular cloning and hormonal regulation of a murine epididymal retinoic acid-binding protein mRNA. Endocrinol, 139, 2971-2981, 1998.

Lareyre, J J, Matt??i, M G, Kasper, S, Newcomer, M E, Ong, D E, Matusik, R J, Orgebin-Crist, M C. Structure and putative function of a murine epididymal retinoic acid-binding protein (mE-RABP). J Reprod Fertil Suppl, 53, 59-65, 1998.

Lareyre, J J, Matt??i, M G, Kasper, S, Ong, D E, Matusik, R J, Orgebin-Crist, M C. Genomic organization and chromosomal localization of the murine epididymal retinoic acid-binding protein (mE-RABP) gene. Mol Reprod Dev, 50(4), 387-95, 1998.

Lareyre, J J, Zheng, W L, Zhao, G Q, Kasper, S, Newcomer, M E, Matusik, R J, Ong, D E, Orgebin-Crist, M C. Molecular cloning and hormonal regulation of a murine epididymal retinoic acid-binding protein messenger ribonucleic acid. Endocrinology, 139(6), 2971-81, 1998.

Snoek, R, Bruchovsky, N, Kasper, S, Matusik, R J, Gleave, M, Sato, N, Mawji, N R, Rennie, P S. Differential transactivation by the androgen receptor in prostate cancer cells. Prostate, 36(4), 256-63, 1998.

Yan, Y, Sheppard, P C, Kasper, S, Lin, L, Hoare, S, Kapoor, A, Dodd, J G, Duckworth, M L, Matusik, R J. Large fragment of the probasin promoter targets high levels of transgene expression to the prostate of transgenic mice. Prostate, 32(2), 129-39, 1997.

Barrios R, Lebovitz RM, Wiseman AL, Weisoly DL, Matusik RJ, DeMayo F and Lieberman MW. Ras T24 driven by a probasin promoter induces prostatic hyperplasia in transgenic mice. Transgenics, 2, 23-28, 1996.

Leonard MP, Sareen S, Hills K, Sheppard PC and Matusik RJ. Early characterization of a novel metastatic disease model of murine neuroblastoma. J. of Urol, 1565, 696-700, 1996.

Snoek, R, Rennie, P S, Kasper, S, Matusik, R J, Bruchovsky, N. Induction of cell-free, in vitro transcription by recombinant androgen receptor peptides. J Steroid Biochem Mol Biol, 59(3-4), 243-50, 1996.

Dub??, J Y, Chapdelaine, P, Gu??rin, S, Leclerc, S, Rennie, P S, Matusik, R J, Tremblay, R R. Search for androgen response elements in the proximal promoter of the canine prostate arginine esterase gene. J Androl, 16(4), 304-11, 1995.

Greenberg, N M, DeMayo, F, Finegold, M J, Medina, D, Tilley, W D, Aspinall, J O, Cunha, G R, Donjacour, A A, Matusik, R J, Rosen, J M. Prostate cancer in a transgenic mouse. Proc Natl Acad Sci U S A, 92(8), 3439-43, 1995. PMCID:42182

Levy I, Matusik RJ, Dodd J, Gallagher R and Meyer F. Joint workshop on laboratory aspects of proposed national prostate cancer screening trial: Part II. The establishment of a biorepository (Laboratory Centre for Disease Control, Health Canada). Chronic Diseases of Canada, 16, 129-130, 1995.

Dedhar, S, Rennie, P S, Shago, M, Hagesteijn, C Y, Yang, H, Filmus, J, Hawley, R G, Bruchovsky, N, Cheng, H, Matusik, R J. Inhibition of nuclear hormone receptor activity by calreticulin. Nature, 367(6462), 480-3, 1994.

Greenberg NM, DeMayo FJ, Sheppard PC, Barrios R, Lebovitz R, Finegold M, Dodd JG, Duckworth ML, Rosen RJ and Matusik R. The rat probasin gene promoter directs hormonally-and developmentally-regulated expression of a heterologous gene specifically to the prostate in transgenic mice. Molecular Endocrinology, 8, 230-239, 1994.

Kasper S, Rennie PS, Bruchovsky N, Sheppard PC, Cheng H, Lin L, Shin RPC, Snoek R and Matusik RJ. Cooperative binding of androgen receptors to two DNA sequences is required for androgen induction of the probasin gene. J. of Biol. Chem, 269, 31763-31769, 1994.

Kazemi-Esfajani P, Beitel L, Trifiro M, Kaufman M, Rennie PS, Sheppard P, Matusik RJ, and Pinsky L. Substitution of valine 865 by methionine or leucine causes complete or partial androgen insensitivity, respectively with distinct androgen receptor phenotype. Molecular Endocrinology, 7, 37-46, 1993.

Rennie PS, Bruchovsky N, Leco KJ, Sheppard PC, McQueen SA, Cheng H, Snoek R, Hamel A, Block ME, MacDonald BS Nickel BE, Chang C, Liao, S, Cattini PA and Matusik RJ. Characterization of two cis-acting elements involved in the androgen regulation of the probasin gene. Molecular Endocrinology, 7, 23-36, 1993.

Berczi, I, Nagy, E, de Toledo, S M, Matusik, R J, Friesen, H G. Pituitary hormones regulate c-myc and DNA synthesis in lymphoid tissue. J Immunol, 146(7), 2201-6, 1991.

Harris, S E, Harris, M A, Johnson, C M, Bean, M F, Dodd, J G, Matusik, R J, Carr, S A, Crabb, J W. Structural characterization of the rat seminal vesicle secretion II protein and gene. J Biol Chem, 265(17), 9896-903, 1990.

Matuo, Y, Adams, P S, Nishi, N, Yasumitsu, H, Crabb, J W, Matusik, R J, McKeehan, W L. The androgen-dependent rat prostate protein, probasin, is a heparin-binding protein that co-purifies with heparin-binding growth factor-1. In Vitro Cell Dev Biol, 25(6), 581-4, 1989.

Spence, A M, Sheppard, P C, Davie, J R, Matuo, Y, Nishi, N, McKeehan, W L, Dodd, J G, Matusik, R J. Regulation of a bifunctional mRNA results in synthesis of secreted and nuclear probasin. Proc Natl Acad Sci U S A, 86(20), 7843-7, 1989. PMCID:298167

Sweetland, R, Sheppard, P C, Dodd, J G, Matusik, R J. Post-castration rebound of an androgen regulated prostatic gene. Mol Cell Biochem, 84(1), 3-15, 1988.

Matusik, R J, Fleming, W H, Hamel, A, Westenbrink, T G, Hrabarchuk, B, MacDonald, R, Ramsey, E, Gartner, J G, Pettigrew, N M, Johnston, B. Expression of the c-myc proto-oncogene in prostatic tissue. Prog Clin Biol Res, 239, 91-112, 1987.

Dodd, J G, Kreis, C, Sheppard, P C, Hamel, A, Matusik, R J. Effect of androgens on mRNA for a secretory protein of rat dorsolateral prostate and seminal vesicles. Mol Cell Endocrinol, 47(3), 191-200, 1986.

Fleming, W H, Hamel, A, MacDonald, R, Ramsey, E, Pettigrew, N M, Johnston, B, Dodd, J G, Matusik, R J. Expression of the c-myc protooncogene in human prostatic carcinoma and benign prostatic hyperplasia. Cancer Res, 46(3), 1535-8, 1986.

Matusik, R J, Kreis, C, McNicol, P, Sweetland, R, Mullin, C, Fleming, W H, Dodd, J G. Regulation of prostatic genes: role of androgens and zinc in gene expression. Biochem Cell Biol, 64(6), 601-7, 1986.

Fleming, W H, Murphy, P R, Murphy, L J, Hatton, T W, Matusik, R J, Friesen, H G. Human growth hormone induces and maintains c-myc gene expression in Nb2 lymphoma cells. Endocrinology, 117(6), 2547-9, 1985.

Dodd, J G, Sheppard, P C, Matusik, R J. Characterization and cloning of rat dorsal prostate mRNAs. Androgen regulation of two closely related abundant mRNAs. J Biol Chem, 258(17), 10731-7, 1983.

Fleming, W H, Pettigrew, N M, Matusik, R J, Friesen, H G. Thymic origin of the prolactin-dependent Nb2 lymphoma cell line. Cancer Res, 42(8), 3138-41, 1982.

Matusik, R J, Rosen, J M. Prolactin regulation of casein gene expression: possible mediators. Endocrinology, 106(1), 252-9, 1980.

Rosen, J M, Matusik, R J, Richards, D A, Gupta, P, Rodgers, J R. Multihormonal regulation of casein gene expression at the transcriptional and posttransciptional levels in the mammary gland. Recent Prog Horm Res, 36, 157-93, 1980.

Guyette, W A, Matusik, R J, Rosen, J M. Prolactin-mediated transcriptional and post-transcriptional control of casein gene expression. Cell, 17(4), 1013-23, 1979.

Matusik, R J, Rosen, J M. Prolactin induction of casein mRNA in organ culture. A model system for studying peptide hormone regulation of gene expression. J Biol Chem, 253(7), 2343-7, 1978.

Matusik, R J, Hilf, R. Relationship of adenosine 3'',5''-cyclic monophosphate and guanosine 3'',5''-cyclic monophosphate to growth of dimethylbenz(a)anthracene-induced mammary tumors in rats. J Natl Cancer Inst, 56(3), 659-61, 1976.

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