Vanderbilt University School of Medicine

Waterman, Michael R. , Ph.D.
Professor of Biochemistry, Emeritus

Lab Url: http://medschool.mc.vanderbilt.edu/watermanlab/

Phone Number: 615-322-3318

Email Address:michael.waterman@vanderbilt.edu

Waterman, Michael's picture

Office Address   Mailing Address

607 Light Hall Vanderbilt University
Nashville, TN 37232-0146

607 Light Hall 0146


Research Keywords
Biochemistry

Research Specialty
Structure, function and regulation of cytochrome P450 enzymes required for lipid biosynthesis and metabolism.

Research Description
Our research focus is on cytochrome P450 (CYP) structure/function. Of particular interest is the P450 required for sterol biosynthesis (CYP51) and the 18 P450 enzymes expressed in the soil bacterium Streptomyces coelicolor.
CYP51
CYP51 (sterol 14a-demethylase) is the most widely expressed member of the CYP (P450) superfamily of genes. It is found in all kingdoms of life, animals, plants, yeast and fungi, lower eukaryotes and a few bacteria including Mycobacterium tuberculosis (MTCYP51). Unlike eukaryotic forms of CYP51, which are membrane bound, the bacterial forms are soluble. Taking advantage of this feature of MTCYP51 we have recently determined the high-resolution crystal structure of MTCYP51 at 2.1 ?.
Sequence alignment of the more than 40 known CYP51 sequences including forms from animals, plants, fungi and bacteria identifies about 40 amino acids that are conserved across more than 400 million years of evolution. We propose that these conserved residues represent the minimal requirement for a sterol 14a-demethylase and have begun a detailed study by mutagenesis in both MTCYP51 and human CYP51 to determine the precise role of each conserved residue in CYP51 structure/function. Combining the biochemical and biophysical properties of these mutants with the known structure of MT CYP51 will permit detailed understanding of why these residues have been conserved over such a long period of evolution
Plant forms of CYP51 have greater substrate specificity than those from other phyla. In fact, certain plant forms (i.e. sorghum) will only use obtusifoliol as substrate. We are using mutagenesis of MTCYP51 and sorghum CYP51 to identify specific residues which influence substrate specificity. Also we will determine the structural basis of azole binding to CYP51. Azole compounds bind directly to the heme iron, inhibiting activity of CYP51 and therefore blocking sterol biosynthesis. Infection by the human pathogen Candida albicans is treated with azole compounds and azole-resistant forms of C. albicans appear which have mutations in the CYP51 gene. We are trying to establish the structural basis for reduced azole binding relative to substrate binding in these mutants
Streptomyces coelicolor P450s
Many bacteria, such as E. coli contain no CYP genes. Others contain one or a very few such genes. However, the actinomycetes Mycobacterium tuberculosis and Streptomyces coelicolor contain 20 and 18 CYP genes, respectively. Being a soil bacterium, Streptomyces has developed two general systems for defense in such a potentially hazardous environment. First, it produces antibiotics which protect it from other organisms which would use Streptomyces as a food source. Strains of Streptomyces produce about 70% of the commercially used antimicrobial compounds for both human and veterinary medicine as well as other important medical compounds such as the antitumor agent, adriamycin. Second, Streptomyces has the capacity to detoxify xenobiotic compounds from its soil environment, thereby protecting itself from mutagens and other poisons. We have begun a long-term project to characterize in detail the structure/function of all 18 CYPs in Streptomyces coelicolor in collaboration with Steve Kelly and David Lamb, University of Wales, Aberystwyth, and Fred Guengerich, Director of the VU Center for Molecular Toxicology. The goals of this project are to develop a rational protocol for design and synthesis of new and potent antibiotics, and to generate modified strains of Streptomyces which can be used for bioremediation of toxic compounds in the environment. The specific aims of this project are: (i) to determine the function of each of the individual S. coelicolor CYPs by disruption of each of the 18 CYP genes and evaluation of resulting phenotypes including ability to kill invading bacteria and identification of changes in profiles of secondary metabolites, (ii) to determine the basis of regulation of CYP expression and activity in S. coelicolor, (iii) to determine the high resolution x-ray structure of substrate-free and substrate-bound forms of the S. coelicolor CYPs, (iv) to establish mutagenesis strategies which will change the activities of the individual CYPs in specific ways, leading to mutant forms of S. coelicolor which will produce novel antibiotics and other medically relevant compounds or will efficiently detoxify mutagens in the environment.

Publications
Gainer, JV, Lipkowitz, MS, Yu, C, Waterman, MR, Dawson, EP, Capdevila, JH, Brown, NJ, , . Association of a CYP4A11 variant and blood pressure in black men. J Am Soc Nephrol, 19(8), 1606-12, 2008. PMCID:2488260

Zhao, B, Lin, X, Lei, L, Lamb, DC, Kelly, SL, Waterman, MR, Cane, DE. Biosynthesis of the sesquiterpene antibiotic albaflavenone in Streptomyces coelicolor A3(2). J Biol Chem, 283(13), 8183-9, 2008. PMCID:2276382

Chun, YJ, Shimada, T, Sanchez-Ponce, R, Martin, MV, Lei, L, Zhao, B, Kelly, SL, Waterman, MR, Lamb, DC, Guengerich, FP. Electron transport pathway for a streptomyces cytochrome P450. Cytochrome P450 105D5-catalyzed fatty acid hydroxylation in streptomyces coelicolor A3(2). J Biol Chem, , , 2007.

Lepesheva, GI, Ott, RD, Hargrove, TY, Kleshchenko, YY, Schuster, I, Nes, WD, Hill, GC, Villalta, F, Waterman, MR. Sterol 14alpha-demethylase as a potential target for antitrypanosomal therapy: enzyme inhibition and parasite cell growth. Chem Biol, 14(11), 1283-93, 2007. PMCID:2324070

Lepesheva, GI, Seliskar, M, Knutson, CG, Stourman, NV, Rozman, D, Waterman, MR. Conformational dynamics in the F/G segment of CYP51 from Mycobacterium tuberculosis monitored by FRET. Arch Biochem Biophys, 464(2), 221-7, 2007.

Lepesheva, GI, Waterman, MR. Sterol 14alpha-demethylase cytochrome P450 (CYP51), a P450 in all biological kingdoms. Biochim Biophys Acta, 1770(3), 467-77, 2007. PMCID:2324071

Zhao, B, Lamb, DC, Lei, L, Kelly, SL, Yuan, H, Hachey, DL, Waterman, MR. Different binding modes of two flaviolin substrate molecules in cytochrome P450 158A1 (CYP158A1) compared to CYP158A2. Biochemistry, 46(30), 8725-33, 2007.

Zhao, B, Waterman, MR. Novel properties of P450s in Streptomyces coelicolor. Drug Metab Rev, 39(2-3), 343-52, 2007.

Arase, M, Waterman, MR, Kagawa, N. Purification and characterization of bovine steroid 21-hydroxylase (P450c21) efficiently expressed in Escherichia coli. Biochem Biophys Res Commun, 344(1), 400-5, 2006.

Chun, YJ, Shimada, T, Waterman, MR, Guengerich, FP. Understanding electron transport systems of Streptomyces cytochrome P450. Biochem Soc Trans, 34(Pt 6), 1183-5, 2006.

Lamb, DC, Guengerich, FP, Kelly, SL, Waterman, MR. Exploiting Streptomyces coelicolor A3(2) P450s as a model for application in drug discovery. Expert Opin Drug Metab Toxicol, 2(1), 27-40, 2006.

Lepesheva, GI, Hargrove, TY, Ott, RD, Nes, WD, Waterman, MR. Biodiversity of CYP51 in trypanosomes. Biochem Soc Trans, 34(Pt 6), 1161-4, 2006.

Lepesheva, GI, Zaitseva, NG, Nes, WD, Zhou, W, Arase, M, Liu, J, Hill, GC, Waterman, MR. CYP51 from Trypanosoma cruzi: a phyla-specific residue in the B'' helix defines substrate preferences of sterol 14alpha-demethylase. J Biol Chem, 281(6), 3577-85, 2006.

Nakagawa, K, Holla, VR, Wei, Y, Wang, WH, Gatica, A, Wei, S, Mei, S, Miller, CM, Cha, DR, Price, E, Zent, R, Pozzi, A, Breyer, MD, Guan, Y, Falck, JR, Waterman, MR, Capdevila, JH. Salt-sensitive hypertension is associated with dysfunctional Cyp4a10 gene and kidney epithelial sodium channel. J Clin Invest, 116(6), 1696-702, 2006. PMCID:1459070

Zhao, B, Waterman, MR, Isin, EM, Sundaramoorthy, M, Podust, LM. Ligand-assisted inhibition in cytochrome P450 158A2 from Streptomyces coelicolor A3(2). Biochemistry, 45(24), 7493-500, 2006.

Zhou, W, Lepesheva, GI, Waterman, MR, Nes, WD. Mechanistic Analysis of a Multiple Product Sterol Methyltransferase Implicated in Ergosterol Biosynthesis in Trypanosoma brucei. J Biol Chem, 281(10), 6290-6, 2006.

Gainer, JV, Bellamine, A, Dawson, EP, Womble, KE, Grant, SW, Wang, Y, Cupples, LA, Guo, CY, Demissie, S, O'Donnell, CJ, Brown, NJ, Waterman, MR, Capdevila, JH. Functional variant of CYP4A11 20-hydroxyeicosatetraenoic acid synthase is associated with essential hypertension. Circulation, 111(1), 63-9, 2005.

Kagawa, N, Senbonmatsu, TA, Satoh, K, Ichihara, K, Yamagata, N, Hatano, O, Saito, T, Nguyen, VQ, Waterman, MR, Price, E, Atkinson, JB, Inagami, T. Tetracycline protects myocardium against ischemic injury. Front Biosci, 10, 608-19, 2005.

Waterman, MR. Professor Howard Mason and oxygen activation. Biochem Biophys Res Commun, 338(1), 7-11, 2005.

Waterman, MR, Lepesheva, GI. Sterol 14 alpha-demethylase, an abundant and essential mixed-function oxidase. Biochem Biophys Res Commun, 338(1), 418-22, 2005.

Zhao, B, Guengerich, FP, Voehler, M, Waterman, MR. Role of active site water molecules and substrate hydroxyl groups in oxygen activation by cytochrome P450 158A2: a new mechanism of proton transfer. J Biol Chem, 280(51), 42188-97, 2005.

Zhao, B, Peter Guengerich, F, Bellamine, A, Lamb, DC, Izumikawa, M, Lei, L, Podust, LM, Sundaramoorthy, M, Kalaitzis, JA, Manmohan Reddy, L, Kelly, SL, Moore, BS, Stec, D, Voehler, M, Falck, JR, Shimada, T, Waterman, MR. Binding of two flaviolin substrate molecules, oxidative coupling, and crystal structure of streptomyces coelicolor A3(2) cytochrome P450 158A2. J Biol Chem, , , 2005.

Bellamine, A, Lepesheva, GI, Waterman, MR. Fluconazole binding and sterol demethylation in three CYP51 isoforms indicate differences in active site topology. J Lipid Res, 45(11), 2000-7, 2004.

Kagawa, N, Hori, H, Waterman, MR, Yoshioka, S. Characterization of stable human aromatase expressed in E. coli. Steroids, 69(4), 235-43, 2004.

Lepesheva, GI, Nes, WD, Zhou, W, Hill, GC, Waterman, MR. CYP51 from Trypanosoma brucei is obtusifoliol-specific. Biochemistry, 43(33), 10789-99, 2004.

Lepesheva, Galina I, Waterman, Michael R. CYP51-the omnipotent P450. Mol Cell Endocrinol, 215(1-2), 165-70, 2004.

Podust, LM, Yermalitskaya, LV, Lepesheva, GI, Podust, VN, Dalmasso, EA, Waterman, MR. Estriol bound and ligand-free structures of sterol 14alpha-demethylase. Structure (Camb), 12(11), 1937-45, 2004.

Podust, Larissa M, Bach, Horacio, Kim, Youngchang, Lamb, David C, Arase, Miharu, Sherman, David H, Kelly, Steven L, Waterman, Michael R. Comparison of the 1.85 A structure of CYP154A1 from Streptomyces coelicolor A3(2) with the closely related CYP154C1 and CYPs from antibiotic biosynthetic pathways. Protein Sci, 13(1), 255-68, 2004. PMCID:2286509

Bellamine, Aouatef, Wang, Yarong, Waterman, Michael R, Gainer, James V, Dawson, Elliot P, Brown, Nancy J, Capdevila, Jorge H. Characterization of the CYP4A11 gene, a second CYP4A gene in humans. Arch Biochem Biophys, 409(1), 221-7, 2003.

Fon Tacer, Klementina, Kalanj-Bognar, Svjetlana, Waterman, Michael R, Rozman, Damjana, . Lanosterol metabolism and sterol regulatory element binding protein (SREBP) expression in male germ cell maturation.. J Steroid Biochem Mol Biol, 85, 429-38, 2003.

Lamb, David C, Ikeda, Haruo, Nelson, David R, Ishikawa, Jun, Skaug, Tove, Jackson, Colin, Omura, Satoshi, Waterman, Michael R, Kelly, Steven L. Cytochrome p450 complement (CYPome) of the avermectin-producer Streptomyces avermitilis and comparison to that of Streptomyces coelicolor A3(2). Biochem Biophys Res Commun, 307(3), 610-9, 2003.

Lepesheva, Galina I, Virus, Cornelia, Waterman, Michael R. Conservation in the CYP51 Family. Role of the B' Helix/BC Loop and Helices F and G in Enzymatic Function. Biochemistry, 42(30), 9091-101, 2003.

Nakagawa, Kiyoshi, Marji, Jackleen S, Schwartzman, Michal L, Waterman, Michael R, Capdevila, Jorge H. Androgen-mediated induction of the kidney arachidonate hydroxylases is associated with the development of hypertension. Am J Physiol Regul Integr Comp Physiol, 284(4), R1055-62, 2003.

Podust, Larissa M, Kim, Youngchang, Arase, Miharu, Neely, Benjamin A, Beck, Brian J, Bach, Horacio, Sherman, David H, Lamb, David C, Kelly, Steven L, Waterman, Michael R. The 1.92-A structure of Streptomyces coelicolor A3(2) CYP154C1. A new monooxygenase that functionalizes macrolide ring systems. J Biol Chem, 278(14), 12214-21, 2003.

Sewer, Marion B, Waterman, Michael R. CAMP-dependent protein kinase enhances CYP17 transcription via MKP-1 activation in H295R human adrenocortical cells. J Biol Chem, 278(10), 8106-11, 2003.

Sewer, Marion B, Waterman, Michael R. ACTH modulation of transcription factors responsible for steroid hydroxylase gene expression in the adrenal cortex. Microsc Res Tech, 61(3), 300-7, 2003.

Halder, Sunil K, Fink, Martina, Waterman, Michael R, Rozman, Damjana. A cAMP-responsive element binding site is essential for sterol regulation of the human lanosterol 14alpha-demethylase gene (CYP51). Mol Endocrinol, 16, 1853-63, 2002.

Lamb, David C, Fowler, Kay, Kieser, Tobias, Manning, Nigel, Podust, Larissa M, Waterman, Michael R, Kelly, Diane E, Kelly, Steven L. Sterol 14alpha-demethylase activity in Streptomyces coelicolor A3(2) is associated with an unusual member of the CYP51 gene family. Biochem J, 364, 555-62, 2002. PMCID:1222601

Lamb, David C, Skaug, Tove, Song, Hong-Lin, Jackson, Colin J, Podust, Larissa M, Waterman, Michael R, Kell, Douglas B, Kelly, Diane E, Kelly, Steven L. The cytochrome P450 complement (CYPome) of Streptomyces coelicolor A3(2). J Biol Chem, 277, 24000-5, 2002.

Sewer, M B, Waterman, M R. Transcriptional complexes at the CYP17 CRS. Endocr Res, 28, 551-8, 2002.

Sewer, M B, Waterman, M R. cAMP-dependent transcription of steroidogenic genes in the human adrenal cortex requires a dual-specificity phosphatase in addition to protein kinase A. J Mol Endocrinol, 29, 163-74, 2002.

Sewer, Marion B, Nguyen, Viet Q, Huang, Ching-Jung, Tucker, Philip W, Kagawa, Norio, Waterman, Michael R. Transcriptional activation of human CYP17 in H295R adrenocortical cells depends on complex formation among p54(nrb)/NonO, protein-associated splicing factor, and SF-1, a complex that also participates in repression of transcription. Endocrinology, 143, 1280-90, 2002.

Sewer, Marion B, Waterman, Michael R. Adrenocorticotropin/cyclic adenosine 3',5'-monophosphate-mediated transcription of the human CYP17 gene in the adrenal cortex is dependent on phosphatase activity. Endocrinology, 143, 1769-77, 2002.

Tom, Moshe, Myers, C R, Waterman, Michael R. Evaluating molar CYP1A level in fish hepatic microsomes by competitive ELISA using recombinant membrane-free CYP1A standard protein. Aquat Toxicol, 59, 101-14, 2002.

Anandatheerthavarada, H K, Amuthan, G, Biswas, G, Robin, M A, Murali, R, Waterman, M R, Avadhani, N G. Evolutionarily divergent electron donor proteins interact with P450MT2 through the same helical domain but different contact points. EMBO J, 20, 2394-403, 2001. PMCID:125462

Bellamine, A, Mangla, A T, Dennis, A L, Nes, W D, Waterman, M R. Structural requirements for substrate recognition of Mycobacterium tuberculosis 14 alpha-demethylase: implications for sterol biosynthesis. J Lipid Res, 42, 128-36, 2001.

Holla, V R, Adas, F, Imig, J D, Zhao, X, Price, E, Olsen, N, Kovacs, W J, Magnuson, M A, Keeney, D S, Breyer, M D, Falck, J R, Waterman, M R, Capdevila, J H. Alterations in the regulation of androgen-sensitive Cyp 4a monooxygenases cause hypertension. Proc Natl Acad Sci U S A, 98, 5211-6, 2001. PMCID:33189

Kusano, K, Kagawa, N, Sakaguchi, M, Omura, T, Waterman, M R. Importance of a proline-rich sequence in the amino-terminal region for correct folding of mitochondrial and soluble microbial p450s. J Biochem (Tokyo), 129, 271-7, 2001.

Kusano, K, Sakaguchi, M, Kagawa, N, Waterman, M R, Omura, T. Microsomal p450s use specific proline-rich sequences for efficient folding, but not for maintenance of the folded structure. J Biochem (Tokyo), 129, 259-69, 2001.

Lepesheva, G I, Podust, L M, Bellamine, A, Waterman, M R. Folding requirements are different between sterol 14alpha-demethylase (CYP51) from Mycobacterium tuberculosis and human or fungal orthologs. J Biol Chem, 276, 28413-20, 2001.

Podust, L M, Poulos, T L, Waterman, M R. Crystal structure of cytochrome P450 14alpha -sterol demethylase (CYP51) from Mycobacterium tuberculosis in complex with azole inhibitors. Proc Natl Acad Sci U S A, 98, 3068-73, 2001. PMCID:30608

Podust, L M, Stojan, J, Poulos, T L, Waterman, M R. Substrate recognition sites in 14alpha-sterol demethylase from comparative analysis of amino acid sequences and X-ray structure of Mycobacterium tuberculosis CYP51. J Inorg Biochem, 87, 227-35, 2001.

Sewer, M B, Waterman, M R. Insights into the transcriptional regulation of steroidogenic enzymes and StAR. Rev Endocr Metab Disord, 2, 269-74, 2001.

Brock, B J, Waterman, M R. The use of random chimeragenesis to study structure/function properties of rat and human P450c17. Arch Biochem Biophys, 373, 401-8, 2000.

Cheng, P Y, Kagawa, N, Takahashi, Y, Waterman, M R. Three zinc finger nuclear proteins, Sp1, Sp3, and a ZBP-89 homologue, bind to the cyclic adenosine monophosphate-responsive sequence of the bovine adrenodoxin gene and regulate transcription. Biochemistry, 39, 4347-57, 2000.

Majdic, G, Parvinen, M, Bellamine, A, Harwood , H J, Ku, W W, Waterman, M R, Rozman, D. Lanosterol 14alpha-demethylase (CYP51), NADPH-cytochrome P450 reductase and squalene synthase in spermatogenesis: late spermatids of the rat express proteins needed to synthesize follicular fluid meiosis activating sterol. J Endocrinol, 166, 463-74, 2000.

Pikuleva, I A, Tesh, K, Waterman, M R, Kim, Y. The tertiary structure of full-length bovine adrenodoxin suggests functional dimers. Arch Biochem Biophys, 373, 44-55, 2000.

Ahlgren, R, Suske, G, Waterman, M R, Lund, J. Role of Sp1 in cAMP-dependent transcriptional regulation of the bovine CYP11A gene. J Biol Chem, 274, 19422-8, 1999.

Bellamine, A, Mangla, A T, Nes, W D, Waterman, M R. Characterization and catalytic properties of the sterol 14alpha-demethylase from Mycobacterium tuberculosis. Proc Natl Acad Sci U S A, 96, 8937-42, 1999. PMCID:17711

Brock, B J, Waterman, M R. Biochemical differences between rat and human cytochrome P450c17 support the different steroidogenic needs of these two species. Biochemistry, 38, 1598-606, 1999.

Jenkins, C M, Waterman, M R. Flavodoxin as a model for the P450-interacting domain of NADPH cytochrome P450 reductase. Drug Metab Rev, 31, 195-203, 1999.

Kagawa, N, Bischof, L J, Cheng, P Y, Anwar, A, Waterman, M R. Biochemical diversity of peptide-hormone-dependent regulation of steroidogenic P450s. Drug Metab Rev, 31, 333-42, 1999.

Keeney, D S, Waterman, M R. Two novel sites of expression of NADPH cytochrome P450 reductase during murine embryogenesis: limb mesenchyme and developing olfactory neuroepithelia. Dev Dyn, 216, 511-7, 1999.

Kusano, K, Waterman, M R, Sakaguchi, M, Omura, T, Kagawa, N. Protein synthesis inhibitors and ethanol selectively enhance heterologous expression of P450s and related proteins in Escherichia coli. Arch Biochem Biophys, 367, 129-36, 1999.

Lamb, D C, Kelly, D E, Waterman, M R, Stromstedt, M, Rozman, D, Kelly, S L. Characteristics of the heterologously expressed human lanosterol 14alpha-demethylase (other names: P45014DM, CYP51, P45051) and inhibition of the purified human and Candida albicans CYP51 with azole antifungal agents. Yeast, 15, 755-63, 1999.

Pikuleva, I A, Cao, C, Waterman, M R. An additional electrostatic interaction between adrenodoxin and P450c27 (CYP27A1) results in tighter binding than between adrenodoxin and p450scc (CYP11A1). J Biol Chem, 274, 2045-52, 1999.

Rozman, D, Fink, M, Fimia, G M, Sassone-Corsi, P, Waterman, M R. Cyclic adenosine 3',5'-monophosphate(cAMP)/cAMP-responsive element modulator (CREM)-dependent regulation of cholesterogenic lanosterol 14alpha-demethylase (CYP51) in spermatids. Mol Endocrinol, 13, 1951-62, 1999.

Tom, M, Waterman, M R, Bocking, D, Dauphin-Villemant, C. Cloning and characterization of the L15 ribosomal protein gene homologue from the crayfish Orconectes limosus. Biochem Biophys Res Commun, 256, 313-6, 1999.

Bischof, L J, Kagawa, N, Moskow, J J, Takahashi, Y, Iwamatsu, A, Buchberg, A M, Waterman, M R. Members of the meis1 and pbx homeodomain protein families cooperatively bind a cAMP-responsive sequence (CRS1) from bovine CYP17. J Biol Chem, 273, 7941-8, 1998.

Bischof, L J, Kagawa, N, Waterman, M R. The bovine CYP17 promoter contains a transcriptional regulatory element cooperatively bound by tale homeodomain proteins. Endocr Res, 24, 489-95, 1998.

Jenkins, C M, Pikuleva, I, Waterman, M R. Expression of eukaryotic cytochromes P450 in E. coli. Methods Mol Biol, 107, 181-93, 1998.

Jenkins, C M, Waterman, M R. NADPH-flavodoxin reductase and flavodoxin from Escherichia coli: characteristics as a soluble microsomal P450 reductase. Biochemistry, 37, 6106-13, 1998.

Keeney, D S, Skinner, C, Travers, J B, Capdevila, J H, Nanney, L B, King, L E, Waterman, M R. Differentiating keratinocytes express a novel cytochrome P450 enzyme, CYP2B19, having arachidonate monooxygenase activity. J Biol Chem, 273, 32071-9, 1998.

Keeney, D S, Skinner, C, Wei, S, Friedberg, T, Waterman, M R. A keratinocyte-specific epoxygenase, CYP2B12, metabolizes arachidonic acid with unusual selectivity, producing a single major epoxyeicosatrienoic acid. J Biol Chem, 273, 9279-84, 1998.

Nonaka, Y, Fujii, T, Kagawa, N, Waterman, M R, Takemori, H, Okamoto, M. Structure/function relationship of CYP11B1 associated with Dahl's salt-resistant rats--expression of rat CYP11B1 and CYP11B2 in Escherichia coli. Eur J Biochem, 258, 869-78, 1998.

Pikuleva, I A, Babiker, A, Waterman, M R, Bjorkhem, I. Activities of recombinant human cytochrome P450c27 (CYP27) which produce intermediates of alternative bile acid biosynthetic pathways. J Biol Chem, 273, 18153-60, 1998.

Rozman, D, Waterman, M R. Lanosterol 14alpha-demethylase (CYP51) and spermatogenesis. Drug Metab Dispos, 26, 1199-201, 1998.

Stromstedt, M, Waterman, M R, Haugen, T B, Tasken K, Parvinen, M, Rozman, D. Elevated expression of lanosterol 14alpha-demethylase (CYP51) and the synthesis of oocyte meiosis-activating sterols in postmeiotic germ cells of male rats. Endocrinology, 139, 2314-21, 1998.

Waterman, M R. Steroidogenesis, StAR and PBR: is there light at the end of the tunnel?. Proc Soc Exp Biol Med, 217, 121-2, 1998.

Bassett, M H, McCarthy, J L, Waterman, M R, Sliter, T J. Sequence and developmental expression of Cyp18, a member of a new cytochrome P450 family from Drosophila. Mol Cell Endocrinol, 131, 39-49, 1997.

Jenkins, C M, Genzor, C G, Fillat, M F, Waterman, M R, Gomez-Moreno, C. Negatively charged anabaena flavodoxin residues (Asp144 and Glu145) are important for reconstitution of cytochrome P450 17alpha-hydroxylase activity. J Biol Chem, 272, 22509-13, 1997.

Jenkins, C M, Pikuleva, I, Kagawa, N, Waterman, M R. Escherichia coli flavodoxin sepharose as an affinity resin for cytochromes P450 and use to identify a putative cytochrome P450c17/3beta-hydroxysteroid dehydrogenase interaction. Arch Biochem Biophys, 347, 93-102, 1997.

Ogo, A, Waterman, M R, Kagawa, N. cAMP-dependent transactivation involving the homeodomain protein Pbx1. Arch Biochem Biophys, 338, 193-200, 1997.

Ogo, A, Waterman, M R, McAllister, J M, Kagawa, N. The homeodomain protein Pbx1 is involved in cAMP-dependent transcription of human CYP17. Arch Biochem Biophys, 348, 226-31, 1997.

Pikuleva, I A, Bjorkhem, I, Waterman, M R. Expression, purification, and enzymatic properties of recombinant human cytochrome P450c27 (CYP27). Arch Biochem Biophys, 343, 123-30, 1997.

Vianello, S, Waterman, M R, Dalla Valle, L, Colombo, L. Developmentally regulated expression and activity of 17alpha-hydroxylase/C-17,20-lyase cytochrome P450 in rat liver. Endocrinology, 138, 3166-74, 1997.

Waterman, M R, Bischof, L J. Cytochromes P450 12: diversity of ACTH (cAMP)-dependent transcription of bovine steroid hydroxylase genes. FASEB J, 11, 419-27, 1997.

Estabrook, R W, Shet, M S, Fisher, C W, Jenkins, C M, Waterman, M R. The interaction of NADPH-P450 reductase with P450: an electrochemical study of the role of the flavin mononucleotide-binding domain. Arch Biochem Biophys, 333, 308-15, 1996.

Nelson, D R, Koymans, L, Kamataki, T, Stegeman, J J, Feyereisen, R, Waxman, D J, Waterman, M R, Gotoh, O, Coon, M J, Estabrook, R W, Gunsalus, I C, Nebert, D W. P450 superfamily: update on new sequences, gene mapping, accession numbers and nomenclature. Pharmacogenetics, 6, 1-42, 1996.

Pikuleva, I A, Bjorkhem, I, Waterman, M R. Studies of distant members of the P450 superfamily (P450scc and P450c27) by random chimeragenesis. Arch Biochem Biophys, 334, 183-92, 1996.

Rozman, D, Stromstedt, M, Tsui, L C, Scherer, S W, Waterman, M R. Structure and mapping of the human lanosterol 14alpha-demethylase gene (CYP51) encoding the cytochrome P450 involved in cholesterol biosynthesis; comparison of exon/intron organization with other mammalian and fungal CYP genes. Genomics, 38, 371-81, 1996.

Rozman, D, Stromstedt, M, Waterman, M R. The three human cytochrome P450 lanosterol 14 alpha-demethylase (CYP51) genes reside on chromosomes 3, 7, and 13: structure of the two retrotransposed pseudogenes, association with a line-1 element, and evolution of the human CYP51 family. Arch Biochem Biophys, 333, 466-74, 1996.

Stromstedt, M, Keeney, D S, Waterman, M R, Paria, B C, Conley, A J, Dey, S K. Preimplantation mouse blastocysts fail to express CYP genes required for estrogen biosynthesis. Mol Reprod Dev, 43, 428-36, 1996.

Stromstedt, M, Rozman, D, Waterman, M R. The ubiquitously expressed human CYP51 encodes lanosterol 14 alpha-demethylase, a cytochrome P450 whose expression is regulated by oxysterols. Arch Biochem Biophys, 329, 73-81, 1996.

Waterman, M R. Introduction: transcription and regulation of activities of cytochromes P450 metabolizing endogenous substrates. FASEB J, 10, 1455, 1996.

Waterman, M R, Bischof, L J. Mechanisms of ACTH(cAMP)-dependent transcription of adrenal steroid hydroxylases. Endocr Res, 22, 615-20, 1996.

Waterman, M R, Keeney, D S. Signal transduction pathways combining peptide hormones and steroidogenesis. Vitam Horm, 52, 129-48, 1996.

Ehrhart-Bornstein, M, Bornstein, S R, Gonzalez-Hernandez, J, Holst, J J, Waterman, M R, Scherbaum, W A. Sympathoadrenal regulation of adrenocortical steroidogenesis. Endocr Res, 21, 13-24, 1995.

Katagiri, M, Kagawa, N, Waterman, M R. The role of cytochrome b5 in the biosynthesis of androgens by human P450c17. Arch Biochem Biophys, 317, 343-7, 1995.

Keeney, D S, Ikeda, Y, Waterman, M R, Parker, K L. Cholesterol side-chain cleavage cytochrome P450 gene expression in the primitive gut of the mouse embryo does not require steroidogenic factor 1. Mol Endocrinol, 9, 1091-8, 1995.

Keeney, D S, Jenkins, C M, Waterman, M R. Developmentally regulated expression of adrenal 17 alpha-hydroxylase cytochrome P450 in the mouse embryo. Endocrinology, 136, 4872-9, 1995.

Ogo, A, Waterman, M R, Kamps, M P, Kagawa, N. Protein kinase A-dependent transactivation by the E2A-Pbx1 fusion protein. J Biol Chem, 270, 25340-3, 1995.

Pikuleva, I A, Mackman, R L, Kagawa, N, Waterman, M R, Ortiz de Montellano, P R. Active-site topology of bovine cholesterol side-chain cleavage cytochrome P450 (P450scc) and evidence for interaction of tyrosine 94 with the side chain of cholesterol. Arch Biochem Biophys, 322, 189-97, 1995.

Stromstedt, M, Waterman, M R. A full-length cDNA encoding mouse adrenodoxin. Biochim Biophys Acta, 1261, 126-8, 1995.

Stromstedt, M, Waterman, M R. Messenger RNAs encoding steroidogenic enzymes are expressed in rodent brain. Brain Res Mol Brain Res, 34, 75-88, 1995.

Venepally, P, Waterman, M R. Two Sp1-binding sites mediate cAMP-induced transcription of the bovine CYP11A gene through the protein kinase A signaling pathway. J Biol Chem, 270, 25402-10, 1995.

Waterman, M R. A rising StAR: an essential role in cholesterol transport. Science, 267, 1780-1, 1995.

Waterman, M R, Jenkins, C M, Pikuleva, I. Genetically engineered bacterial cells and applications. Toxicol Lett, 82-83, 807-13, 1995.

Barnes, H J, Jenkins, C M, Waterman, M R. Baculovirus expression of bovine cytochrome P450c17 in Sf9 cells and comparison with expression in yeast, mammalian cells, and E. coli. Arch Biochem Biophys, 315(2), 489-94, 1994.

Hatano, O, Takayama, K, Imai, T, Waterman, M R, Takakusu, A, Omura, T, Morohashi, K. Sex-dependent expression of a transcription factor, Ad4BP, regulating steroidogenic P-450 genes in the gonads during prenatal and postnatal rat development. Development, 120(10), 2787-97, 1994.

Jenkins, C M, Waterman, M R. Flavodoxin and NADPH-flavodoxin reductase from Escherichia coli support bovine cytochrome P450c17 hydroxylase activities. J Biol Chem, 269(44), 27401-8, 1994.

Kagawa, N, Ogo, A, Takahashi, Y, Iwamatsu, A, Waterman, M R. A cAMP-regulatory sequence (CRS1) of CYP17 is a cellular target for the homeodomain protein Pbx1. J Biol Chem, 269(29), 18716-9, 1994.

Sakai, Y, Yanase, T, Okabe, Y, Hara, T, Waterman, M R, Takayanagi, R, Haji, M, Nawata, H. No mutation in cytochrome P450 side chain cleavage in a patient with congenital lipoid adrenal hyperplasia. J Clin Endocrinol Metab, 79(4), 1198-201, 1994.

Waterman, M R. Biochemical diversity of cAMP-dependent transcription of steroid hydroxylase genes in the adrenal cortex. J Biol Chem, 269(45), 27783-6, 1994.

Waterman, M R. Heterologous expression of mammalian P450 enzymes. Adv Enzymol Relat Areas Mol Biol, 68, 37-66, 1994.

Fevold, H R, Ivanovitch, J D, Zanger, U M, Waterman, M R. The sequence of the 5'-end of the rat CYP17 gene, the transcription initiation site and a comparison with the homologous genes of other species. Mol Cell Endocrinol, 95(1-2), 95-100, 1993.

Imai, T, Globerman, H, Gertner, J M, Kagawa, N, Waterman, M R. Expression and purification of functional human 17 alpha-hydroxylase/17,20-lyase (P450c17) in Escherichia coli. Use of this system for study of a novel form of combined 17 alpha-hydroxylase/17,20-lyase deficiency. J Biol Chem, 268(26), 19681-9, 1993.

Keeney, D S, Waterman, M R. Regulation of steroid hydroxylase gene expression: importance to physiology and disease. Pharmacol Ther, 58(3), 301-17, 1993.

Lauber, M E, Kagawa, N, Waterman, M R, Simpson, E R. cAMP-dependent and tissue-specific expression of genes encoding steroidogenic enzymes in bovine luteal and granulosa cells in primary culture. Mol Cell Endocrinol, 93(2), 227-33, 1993.

Lauber, M E, Picton, H M, Begeot, M, Momoi, K, Waterman, M R, Simpson, E R. Regulation of CYP11A gene expression in bovine ovarian granulosa cells in primary culture by cAMP and phorbol esters is conferred by a common cis-acting element. Mol Cell Endocrinol, 94(2), 235-42, 1993.

Morohashi, K, Zanger, U M, Honda, S, Hara, M, Waterman, M R, Omura, T. Activation of CYP11A and CYP11B gene promoters by the steroidogenic cell-specific transcription factor, Ad4BP. Mol Endocrinol, 7(9), 1196-204, 1993.

Richardson, T H, Hsu, M H, Kronbach, T, Barnes, H J, Chan, G, Waterman, M R, Kemper, B, Johnson, E F. Purification and characterization of recombinant-expressed cytochrome P450 2C3 from Escherichia coli: 2C3 encodes the 6 beta-hydroxylase deficient form of P450 3b. Arch Biochem Biophys, 300(1), 510-6, 1993.

Sagara, Y, Barnes, H J, Waterman, M R. Expression in Escherichia coli of functional cytochrome P450c17 lacking its hydrophobic amino-terminal signal anchor. Arch Biochem Biophys, 304(1), 272-8, 1993.

Sagara, Y, Wada, A, Takata, Y, Waterman, M R, Sekimizu, K, Horiuchi, T. Direct expression of adrenodoxin reductase in Escherichia coli and the functional characterization. Biol Pharm Bull, 16(7), 627-30, 1993.

Swart, P, Swart, A C, Waterman, M R, Estabrook, R W, Mason, J I. Progesterone 16 alpha-hydroxylase activity is catalyzed by human cytochrome P450 17 alpha-hydroxylase. J Clin Endocrinol Metab, 77(1), 98-102, 1993.

Trzeciak, W H, LeHoux, J G, Waterman, M R, Simpson, E R. Dexamethasone inhibits corticotropin-induced accumulation of CYP11A and CYP17 messenger RNAs in bovine adrenocortical cells. Mol Endocrinol, 7(2), 206-13, 1993.

Waterman, M R. Heterologous expression of cytochrome P-450 in Escherichia coli. Biochem Soc Trans, 21(4), 1081-5, 1993.

Ahlgren, R, Yanase, T, Simpson, E R, Winter, J S, Waterman, M R. Compound heterozygous mutations (Arg 239----stop, Pro 342----Thr) in the CYP17 (P45017 alpha) gene lead to ambiguous external genitalia in a male patient with partial combined 17 alpha-hydroxylase/17,20-lyase deficiency. J Clin Endocrinol Metab, 74(3), 667-72, 1992.

Chen, J Y, Waterman, M R. Two promoters in the bovine adrenodoxin gene and the role of associated, unique cAMP-responsive sequences. Biochemistry, 31(8), 2400-7, 1992.

Clark, B J, Waterman, M R. Functional expression of bovine 17 alpha-hydroxylase in COS 1 cells is dependent upon the presence of an amino-terminal signal anchor sequence. J Biol Chem, 267(34), 24568-74, 1992.

Durkee, T J, McLean, M P, Hales, D B, Payne, A H, Waterman, M R, Khan, I, Gibori, G. P450(17 alpha) and P450SCC gene expression and regulation in the rat placenta. Endocrinology, 130(3), 1309-17, 1992.

Fan, Y S, Sasi, R, Lee, C, Winter, J S, Waterman, M R, Lin, C C. Localization of the human CYP17 gene (cytochrome P450(17 alpha)) to 10q24.3 by fluorescence in situ hybridization and simultaneous chromosome banding. Genomics, 14(4), 1110-1, 1992.

Fisher, C W, Caudle, D L, Martin-Wixtrom, C, Quattrochi, L C, Tukey, R H, Waterman, M R, Estabrook, R W. High-level expression of functional human cytochrome P450 1A2 in Escherichia coli. FASEB J, 6(2), 759-64, 1992.

G??se-Behling, H, Ehrhart-Bornstein, M, Bornstein, S R, Waterman, M R, Scherbaum, W A, Adler, G. Regulation of adrenal steroidogenesis by adrenaline: expression of cytochrome P450 genes. J Endocrinol, 135(2), 229-37, 1992.

Imai, T, Yanase, T, Waterman, M R, Simpson, E R, Pratt, J J. Canadian Mennonites and individuals residing in the Friesland region of The Netherlands share the same molecular basis of 17 alpha-hydroxylase deficiency. Hum Genet, 89(1), 95-6, 1992.

Kagawa, N, Waterman, M R. Purification and characterization of a transcription factor which appears to regulate cAMP responsiveness of the human CYP21B gene. J Biol Chem, 267(35), 25213-9, 1992.

Momoi, K, Waterman, M R, Simpson, E R, Zanger, U M. 3',5'-cyclic adenosine monophosphate-dependent transcription of the CYP11A (cholesterol side chain cleavage cytochrome P450) gene involves a DNA response element containing a putative binding site for transcription factor Sp1. Mol Endocrinol, 6(10), 1682-90, 1992.

Palin, M F, Berthiaume, L, Lehoux, J G, Waterman, M R, Sygusch, J. Direct expression of mature bovine adrenodoxin in Escherichia coli. Arch Biochem Biophys, 295(1), 126-31, 1992.

Wada, A, Waterman, M R. Identification by site-directed mutagenesis of two lysine residues in cholesterol side chain cleavage cytochrome P450 that are essential for adrenodoxin binding. J Biol Chem, 267(32), 22877-82, 1992.

Waterman, M R, Keeney, D S. Genes involved in androgen biosynthesis and the male phenotype. Horm Res, 38(5-6), 217-21, 1992.

Yanase, T, Waterman, M R, Zachmann, M, Winter, J S, Simpson, E R, Kagimoto, M. Molecular basis of apparent isolated 17,20-lyase deficiency: compound heterozygous mutations in the C-terminal region (Arg(496)----Cys, Gln(461)----Stop) actually cause combined 17 alpha-hydroxylase/17,20-lyase deficiency. Biochim Biophys Acta, 1139(4), 275-9, 1992.

Zanger, U M, Kagawa, N, Lund, J, Waterman, M R. Distinct biochemical mechanisms for cAMP-dependent transcription of CYP17 and CYP21. FASEB J, 6(2), 719-23, 1992.

Barnes, H J, Arlotto, M P, Waterman, M R. Expression and enzymatic activity of recombinant cytochrome P450 17 alpha-hydroxylase in Escherichia coli. Proc Natl Acad Sci U S A, 88(13), 5597-601, 1991. PMCID:51924

Biason, A, Mantero, F, Scaroni, C, Simpson, E R, Waterman, M R. Deletion within the CYP17 gene together with insertion of foreign DNA is the cause of combined complete 17 alpha-hydroxylase/17,20-lyase deficiency in an Italian patient. Mol Endocrinol, 5(12), 2037-45, 1991.

Clark, B J, Waterman, M R. The hydrophobic amino-terminal sequence of bovine 17 alpha-hydroxylase is required for the expression of a functional hemoprotein in COS 1 cells. J Biol Chem, 266(9), 5898-904, 1991.

Clark, B J, Waterman, M R. Heterologous expression of mammalian P450 in COS cells. Methods Enzymol, 206, 100-8, 1991.

Ehrhart-Bornstein, M, Bornstein, S R, Trzeclak, W H, Usadel, H, G??se-Behling, H, Waterman, M R, Scherbaum, W A. Adrenaline stimulates cholesterol side-chain cleavage cytochrome P450 mRNA accumulation in bovine adrenocortical cells. J Endocrinol, 131(2), R5-8, 1991.

Estabrook, R W, Mason, J I, Simpson, E R, Peterson, J A, Waterman, M R. The heterologous expression of the cytochromes P450: a new approach for the study of enzyme activities and regulation. Adv Enzyme Regul, 31, 365-83, 1991.

Kagawa, N, Waterman, M R. Evidence that an adrenal-specific nuclear protein regulates the cAMP responsiveness of the human CYP21B (P450C21) gene. J Biol Chem, 266(17), 11199-204, 1991.

Lauber, M E, Bengtson, T, Waterman, M R, Simpson, E R. Regulation of CYP11A (P450SCC) and CYP17 (P450(17) alpha) gene expression in bovine luteal cells in primary culture. J Biol Chem, 266(17), 11170-5, 1991.

Lauber, M E, Waterman, M R, Simpson, E R. Expression of genes encoding steroidogenic enzymes in the bovine corpus luteum. J Reprod Fertil Suppl, 43, 57-64, 1991.

Stirling, D, Waterman, M R, Simpson, E R. Expression of mRNA encoding basic fibroblast growth factor (bFGF) in bovine corpora lutea and cultured luteal cells. J Reprod Fertil, 91(1), 1-8, 1991.

Tremblay, A, Waterman, M R, Parker, K L, Lehoux, J G. Regulation of rat adrenal messenger RNA and protein levels for cytochrome P-450s and adrenodoxin by dietary sodium depletion or potassium intake. J Biol Chem, 266(4), 2245-51, 1991.

Wada, A, Mathew, P A, Barnes, H J, Sanders, D, Estabrook, R W, Waterman, M R. Expression of functional bovine cholesterol side chain cleavage cytochrome P450 (P450scc) in Escherichia coli. Arch Biochem Biophys, 290(2), 376-80, 1991.

Yanase, T, Simpson, E R, Waterman, M R. 17 alpha-hydroxylase/17,20-lyase deficiency: from clinical investigation to molecular definition. Endocr Rev, 12(1), 91-108, 1991.

Zanger, U M, Lund, J, Simpson, E R, Waterman, M R. Activation of transcription in cell-free extracts by a novel cAMP-responsive sequence from the bovine CYP17 gene. J Biol Chem, 266(18), 11417-20, 1991.

Ahlgren, R, Simpson, E R, Waterman, M R, Lund, J. Characterization of the promoter/regulatory region of the bovine CYP11A (P-450scc) gene. Basal and cAMP-dependent expression. J Biol Chem, 265(6), 3313-9, 1990.

Kagawa, N, Waterman, M R. cAMP-dependent transcription of the human CYP21B (P-450C21) gene requires a cis-regulatory element distinct from the consensus cAMP-regulatory element. J Biol Chem, 265(19), 11299-305, 1990.

Lorence, M C, Trant, J M, Clark, B J, Khyatt, B, Mason, J I, Estabrook, R W, Waterman, M R. Construction and expression of human/bovine P45017 alpha chimeric proteins: evidence for distinct tertiary structures in the same P450 from two different species. Biochemistry, 29(42), 9819-24, 1990.

Lund, J, Ahlgren, R, Wu, D H, Kagimoto, M, Simpson, E R, Waterman, M R. Transcriptional regulation of the bovine CYP17 (P-450(17)alpha) gene. Identification of two cAMP regulatory regions lacking the consensus cAMP-responsive element (CRE). J Biol Chem, 265(6), 3304-12, 1990.

Mathew, P A, Kagawa, N, Bhasker, C R, Waterman, M R. Deduced amino acid sequence of heme binding region of chicken cholesterol side chain cleavage cytochrome P450. Protein Seq Data Anal, 3(4), 323-5, 1990.

Mathew, P A, Mason, J I, Trant, J M, Sanders, D, Waterman, M R. Amino acid substitutions Phe66----Leu and Ser126----Pro abolish cortisol and aldosterone synthesis by bovine cytochrome P450(11)beta. J Biol Chem, 265(33), 20228-33, 1990.

Mathew, P A, Mason, J I, Trant, J M, Waterman, M R. Incorporation of steroidogenic pathways which produce cortisol and aldosterone from cholesterol into nonsteroidogenic cells. Mol Cell Endocrinol, 73(1), 73-80, 1990.

McAllister, J M, Mason, J I, Byrd, W, Trant, J M, Waterman, M R, Simpson, E R. Proliferating human granulosa-lutein cells in long term monolayer culture: expression of aromatase, cholesterol side-chain cleavage, and 3 beta-hydroxysteroid dehydrogenase. J Clin Endocrinol Metab, 71(1), 26-33, 1990.

Simpson, E R, Lund, J, Ahlgren, R, Waterman, M R. Regulation by cyclic AMP of the genes encoding steroidogenic enzymes: when the light finally shines. Mol Cell Endocrinol, 70(2), C25-8, 1990.

Stirling, D, Magness, R R, Stone, R, Waterman, M R, Simpson, E R. Angiotensin II inhibits luteinizing hormone-stimulated cholesterol side chain cleavage expression and stimulates basic fibroblast growth factor expression in bovine luteal cells in primary culture. J Biol Chem, 265(1), 5-8, 1990.

Waterman, M R, Lund, J, Ahlgren, R, Wu, D, Simpson, E R. Regulation of steroid hydroxylase gene expression and steroid hormone biosynthesis. Biochem Soc Trans, 18(1), 26-8, 1990.

Waterman, M R, Simpson, E R. Steroidogenic capacity in the adrenal cortex and its regulation. Prog Drug Res, 34, 359-81, 1990.

Yanase, T, Sanders, D, Shibata, A, Matsui, N, Simpson, E R, Waterman, M R. Combined 17 alpha-hydroxylase/17,20-lyase deficiency due to a 7-basepair duplication in the N-terminal region of the cytochrome P45017 alpha (CYP17) gene. J Clin Endocrinol Metab, 70(5), 1325-9, 1990.

Bhasker, C R, Adler, B S, Dee, A, John, M E, Kagimoto, M, Zuber, M X, Ahlgren, R, Wang, X D, Simpson, E R, Waterman, M R. Structural characterization of the bovine CYP17 (17 alpha-hydroxylase) gene. Arch Biochem Biophys, 271(2), 479-87, 1989.

Boggaram, V, John, M E, Simpson, E R, Waterman, M R. Effect of ACTH on the stability of mRNAs encoding bovine adrenocortical P-450scc, P-45011 beta, P-45017 alpha, P-450C21 and adrenodoxin. Biochem Biophys Res Commun, 160(3), 1227-32, 1989.

Fevold, H R, Lorence, M C, McCarthy, J L, Trant, J M, Kagimoto, M, Waterman, M R, Mason, J I. Rat P450(17 alpha) from testis: characterization of a full-length cDNA encoding a unique steroid hydroxylase capable of catalyzing both delta 4- and delta 5-steroid-17,20-lyase reactions. Mol Endocrinol, 3(6), 968-75, 1989.

Kagimoto, K, Waterman, M R, Kagimoto, M, Ferreira, P, Simpson, E R, Winter, J S. Identification of a common molecular basis for combined 17 alpha-hydroxylase/17,20-lyase deficiency in two Mennonite families. Hum Genet, 82(3), 285-6, 1989.

Lorence, M C, Trant, J M, Mason, J I, Bhasker, C R, Fujii-Kuriyama, Y, Estabrook, R W, Waterman, M R. Expression of a full-length cDNA encoding bovine adrenal cytochrome P450C21. Arch Biochem Biophys, 273(1), 79-88, 1989.

Lund, J, Mason, J I, Simpson, E R, Waterman, M R. Developmental regulation of P-45017 alpha gene expression in fetal bovine adrenal. Endocr Res, 15(1-2), 17-29, 1989.

McAllister, J M, Kerin, J F, Trant, J M, Estabrook, R W, Mason, J I, Waterman, M R, Simpson, E R. Regulation of cholesterol side-chain cleavage and 17 alpha-hydroxylase/lyase activities in proliferating human theca interna cells in long term monolayer culture. Endocrinology, 125(4), 1959-66, 1989.

Swart, P, Estabrook, R W, Mason, J I, Waterman, M R. Catalytic activity of human and bovine adrenal cytochromes P-450 17 alpha, lyase expressed in Cos 1 cells. Biochem Soc Trans, 17(6), 1025-6, 1989.

Waterman, M R, Mason, J I, Zuber, M X, Lorence, M C, Clark, B J, Trant, J M, Barnes, H J, Simpson, E R, Estabrook, R W. Expression of P-450 enzyme activities in heterologous cells by transfection. Arch Toxicol Suppl, 13, 155-63, 1989.

Waterman, M R, Simpson, E R. Regulation of steroid hydroxylase gene expression is multifactorial in nature. Recent Prog Horm Res, 45, 533-63; discussion 563-6, 1989.

Yanase, T, Kagimoto, M, Suzuki, S, Hashiba, K, Simpson, E R, Waterman, M R. Deletion of a phenylalanine in the N-terminal region of human cytochrome P-450(17 alpha) results in partial combined 17 alpha-hydroxylase/17,20-lyase deficiency. J Biol Chem, 264(30), 18076-82, 1989.

Brownie, A C, Bhasker, C R, Waterman, M R. Levels of adrenodoxin, NADPH-cytochrome P-450 reductase and cytochromes P-45011 beta, P-45021, P-450scc, in adrenal zona fasciculata-reticularis tissue from androgen-treated rats. Mol Cell Endocrinol, 55(1), 15-20, 1988.

Estabrook, R W, Mason, J I, Martin-Wixtrom, C, Zuber, M, Waterman, M R. Some enzymatic vagaries of a bovine adrenal microsomal cytochrome P-450 introduced and expressed in transformed monkey kidney cells. Prog Clin Biol Res, 274, 525-40, 1988.

Kagimoto, K, McCarthy, J L, Waterman, M R, Kagimoto, M. Deduced amino acid sequence of mature chicken testis ferredoxin. Biochem Biophys Res Commun, 155(1), 379-83, 1988.

Kagimoto, M, Kagimoto, K, Simpson, E R, Waterman, M R. Transcription of the bovine adrenodoxin gene produces two species of mRNA of which only one is translated into adrenodoxin. J Biol Chem, 263(18), 8925-8, 1988.

Kagimoto, M, Winter, J S, Kagimoto, K, Simpson, E R, Waterman, M R. Structural characterization of normal and mutant human steroid 17 alpha-hydroxylase genes: molecular basis of one example of combined 17 alpha-hydroxylase/17,20 lyase deficiency. Mol Endocrinol, 2(6), 564-70, 1988.

Lund, J, Faucher, D J, Ford, S P, Porter, J C, Waterman, M R, Mason, J I. Developmental expression of bovine adrenocortical steroid hydroxylases. Regulation of P-450(17 alpha) expression leads to episodic fetal cortisol production. J Biol Chem, 263(31), 16195-201, 1988.

McCarthy, J L, Waterman, M R. Co-induction of 17 alpha-hydroxylase and C-17,20-lyase activities in primary cultures of bovine adrenocortical cells in response to ACTH treatment. J Steroid Biochem, 29(3), 307-12, 1988.

Nishihara, M, Winters, C A, Buzko, E, Waterman, M R, Dufau, M L. Hormonal regulation of rat Leydig cell cytochrome P-45017 alpha mRNA levels and characterization of a partial length rat P-45017 alpha cDNA. Biochem Biophys Res Commun, 154(1), 151-8, 1988.

Rodgers, R J, Waterman, M R, Simpson, E R, Magness, R R. Immunoblot analysis of cholesterol side-chain cleavage cytochrome P-450 and adrenodoxin in corpora lutea of cyclic and late-pregnant sheep. J Reprod Fertil, 83(2), 843-50, 1988.

Simpson, E R, Waterman, M R. Regulation of the synthesis of steroidogenic enzymes in adrenal cortical cells by ACTH. Annu Rev Physiol, 50, 427-40, 1988.

Waterman, M R, Mason, J I, Simpson, E R. Regulation of steroid hydroxylase gene expression. Prog Clin Biol Res, 274, 543-55, 1988.

Yanase, T, Kagimoto, M, Matsui, N, Simpson, E R, Waterman, M R. Combined 17 alpha-hydroxylase/17,20-lyase deficiency due to a stop codon in the N-terminal region of 17 alpha-hydroxylase cytochrome P-450. Mol Cell Endocrinol, 59(3), 249-53, 1988.

Zuber, M X, Mason, J I, Simpson, E R, Waterman, M R. Simultaneous transfection of COS-1 cells with mitochondrial and microsomal steroid hydroxylases: incorporation of a steroidogenic pathway into nonsteroidogenic cells. Proc Natl Acad Sci U S A, 85(3), 699-703, 1988. PMCID:279622

Bhasker, C R, Okamura, T, Simpson, E R, Waterman, M R. Mature bovine adrenodoxin contains a 14-amino-acid COOH-terminal extension originally detected by cDNA sequencing. Eur J Biochem, 164(1), 21-5, 1987.

Bradshaw, K D, Waterman, M R, Couch, R T, Simpson, E R, Zuber, M X. Characterization of complementary deoxyribonucleic acid for human adrenocortical 17 alpha-hydroxylase: a probe for analysis of 17 alpha-hydroxylase deficiency. Mol Endocrinol, 1(5), 348-54, 1987.

John, M E, Simpson, E R, Carr, B R, Magness, R R, Rosenfeld, C R, Waterman, M R, Mason, J I. Ontogeny of adrenal steroid hydroxylases: evidence for cAMP-independent gene expression. Mol Cell Endocrinol, 50(3), 263-8, 1987.

Muerhoff, A S, Williams, D E, Leithauser, M T, Jackson, V E, Waterman, M R, Masters, B S. Regulation of the induction of a cytochrome P-450 prostaglandin omega-hydroxylase by pregnancy in rabbit lung. Proc Natl Acad Sci U S A, 84(22), 7911-4, 1987. PMCID:299445

Okamura, T, Kagimoto, M, Simpson, E R, Waterman, M R. Multiple species of bovine adrenodoxin mRNA. Occurrence of two different mitochondrial precursor sequences associated with the same mature sequence. J Biol Chem, 262(21), 10335-8, 1987.

Rodgers, R J, Mason, J I, Waterman, M R, Simpson, E R. Regulation of the synthesis of 3-hydroxy-3-methylglutaryl coenzyme A reductase in the bovine ovary in vivo and in vitro. Mol Endocrinol, 1(2), 172-80, 1987.

Rodgers, R J, Waterman, M R, Simpson, E R. Levels of messenger ribonucleic acid encoding cholesterol side-chain cleavage cytochrome P-450, 17 alpha-hydroxylase cytochrome P-450, adrenodoxin, and low density lipoprotein receptor in bovine follicles and corpora lutea throughout the ovarian cycle. Mol Endocrinol, 1(3), 274-9, 1987.

Simpson, E R, Mason, J I, John, M E, Zuber, M X, Rodgers, R J, Waterman, M R. Regulation of the biosynthesis of steroidogenic enzymes. J Steroid Biochem, 27(4-6), 801-5, 1987.

Trzeciak, W H, Duda, T, Waterman, M R, Simpson, E R. Effects of epidermal growth factor on the synthesis of the cholesterol side-chain cleavage enzyme complex in rat ovarian granulosa cells in primary culture. Mol Cell Endocrinol, 52(1-2), 43-50, 1987.

Trzeciak, W H, Duda, T, Waterman, M R, Simpson, E R. Tetradecanoyl phorbol acetate suppresses follicle-stimulating hormone-induced synthesis of the cholesterol side-chain cleavage enzyme complex in rat ovarian granulosa cells. J Biol Chem, 262(31), 15246-50, 1987.

Trzeciak, W H, Simpson, E R, Scallen, T J, Vahouny, G V, Waterman, M R. Studies on the synthesis of sterol carrier protein-2 in rat adrenocortical cells in monolayer culture. Regulation by ACTH and dibutyryl cyclic 3',5'-AMP. J Biol Chem, 262(8), 3713-7, 1987.

Trzeciak, W H, Waterman, M R, Simpson, E R, Ojeda, S R. Vasoactive intestinal peptide regulates cholesterol side-chain cleavage cytochrome P-450 (P-450scc) gene expression in granulosa cells from immature rat ovaries. Mol Endocrinol, 1(7), 500-4, 1987.

John, M E, John, M C, Boggaram, V, Simpson, E R, Waterman, M R. Transcriptional regulation of steroid hydroxylase genes by corticotropin. Proc Natl Acad Sci U S A, 83(13), 4715-9, 1986. PMCID:323812

John, M E, Okamura, T, Dee, A, Adler, B, John, M C, White, P C, Simpson, E R, Waterman, M R. Bovine steroid 21-hydroxylase: regulation of biosynthesis. Biochemistry, 25(10), 2846-53, 1986.

John, M E, Simpson, E R, Waterman, M R, Mason, J I. Regulation of cholesterol side-chain cleavage cytochrome P-450 gene expression in adrenal cells in monolayer culture. Mol Cell Endocrinol, 45(2-3), 197-204, 1986.

Matocha, M F, Waterman, M R. Import and processing of P-450SCC and P-450(11) beta precursors by corpus luteal mitochondria: a processing pathway recognizing homologous and heterologous precursors. Arch Biochem Biophys, 250(2), 456-60, 1986.

Rodgers, R J, Rodgers, H F, Hall, P F, Waterman, M R, Simpson, E R. Immunolocalization of cholesterol side-chain-cleavage cytochrome P-450 and 17 alpha-hydroxylase cytochrome P-450 in bovine ovarian follicles. J Reprod Fertil, 78(2), 627-38, 1986.

Rodgers, R J, Rodgers, H F, Waterman, M R, Simpson, E R. Immunolocalization of cholesterol side-chain-cleavage cytochrome P-450 and ultrastructural studies of bovine corpora lutea. J Reprod Fertil, 78(2), 639-52, 1986.

Rodgers, R J, Waterman, M R, Simpson, E R. Cytochromes P-450scc, P-450(17)alpha, adrenodoxin, and reduced nicotinamide adenine dinucleotide phosphate-cytochrome P-450 reductase in bovine follicles and corpora lutea. Changes in specific contents during the ovarian cycle. Endocrinology, 118(4), 1366-74, 1986.

Sasaki, J, Waterman, M R, Cottam, G L. Decreased apolipoprotein A-I and B content in plasma of individuals with sickle cell anemia. Clin Chem, 32(1 Pt 1), 226-7, 1986.

Trzeciak, W H, Waterman, M R, Simpson, E R. Synthesis of the cholesterol side-chain cleavage enzymes in cultured rat ovarian granulosa cells: induction by follicle-stimulating hormone and dibutyryl adenosine 3',5'-monophosphate. Endocrinology, 119(1), 323-30, 1986.

Waterman, M R, Mason, J I, Zuber, M X, John, M E, Rodgers, R J, Simpson, E R. Control of gene expression of adrenal steroid hydroxylases and related enzymes. Endocr Res, 12(4), 393-408, 1986.

Zuber, M X, John, M E, Okamura, T, Simpson, E R, Waterman, M R. Bovine adrenocortical cytochrome P-450(17 alpha). Regulation of gene expression by ACTH and elucidation of primary sequence. J Biol Chem, 261(5), 2475-82, 1986.

Zuber, M X, Simpson, E R, Waterman, M R. Expression of bovine 17 alpha-hydroxylase cytochrome P-450 cDNA in nonsteroidogenic (COS 1) cells. Science, 234(4781), 1258-61, 1986.

Boggaram, V, Horowitz, P, Waterman, M R. Studies on rhodanese synthesis in bovine adrenocortical cells. Biochem Biophys Res Commun, 130(1), 407-11, 1985.

Dee, A, Carlson, G, Smith, C, Masters, B S, Waterman, M R. Regulation of synthesis and activity of bovine adrenocortical NADPH-cytochrome P-450 reductase by ACTH. Biochem Biophys Res Commun, 128(2), 650-6, 1985.

John, M E, John, M C, Simpson, E R, Waterman, M R. Regulation of cytochrome P-45011 beta gene expression by adrenocorticotropin. J Biol Chem, 260(9), 5760-7, 1985.

Marston, S A, Waterman, M R, McCarthy, J L. 3 Beta-hydroxysteroid dehydrogenase-isomerase activity in bovine adrenocortical cells in culture: lack of response to ACTH treatment. J Steroid Biochem, 22(6), 789-94, 1985.

Matocha, M F, Waterman, M R. Synthesis and processing of mitochondrial steroid hydroxylases. In vivo maturation of the precursor forms of cytochrome P-450scc, cytochrome P-450(11)beta, and adrenodoxin. J Biol Chem, 260(22), 12259-65, 1985.

Okamura, T, John, M E, Zuber, M X, Simpson, E R, Waterman, M R. Molecular cloning and amino acid sequence of the precursor form of bovine adrenodoxin: evidence for a previously unidentified COOH-terminal peptide. Proc Natl Acad Sci U S A, 82(17), 5705-9, 1985. PMCID:390620

Sheets, J J, Zuber, M X, McCarthy, J L, Vickery, L E, Waterman, M R. Discriminatory inhibition of adrenocortical 17 alpha-hydroxylase activity by inhibitors of cholesterol side chain cleavage cytochrome P-450. Arch Biochem Biophys, 242(1), 297-305, 1985.

Simpson, E R, Boggaram, V, Funkenstein, B, Waterman, M R. Lipoproteins and the regulation of steroidogenesis. Biochem Soc Trans, 13(1), 55-8, 1985.

Waterman, M R, Simpson, E R. Regulation of the biosynthesis of cytochromes P-450 involved in steroid hormone synthesis. Mol Cell Endocrinol, 39(2), 81-9, 1985.

Zuber, M X, Simpson, E R, Hall, P F, Waterman, M R. Effects of adrenocorticotropin on 17 alpha-hydroxylase activity and cytochrome P-450(17 alpha) synthesis in bovine adrenocortical cells. J Biol Chem, 260(3), 1842-8, 1985.

Zuber, M X, Simpson, E R, Waterman, M R. Regulation of cytochrome P-450(17)alpha activity and synthesis in bovine adrenocortical cells. Ann N Y Acad Sci, 458, 252-61, 1985.

Boggaram, V, Funkenstein, B, Waterman, M R, Simpson, E R. Lipoproteins and the regulation of adrenal steroidogenesis. Endocr Res, 10(3-4), 387-409, 1984.

Boggaram, V, Simpson, E R, Waterman, M R. Induction of synthesis of bovine adrenocortical cytochromes P-450scc, P-45011 beta, P-450C21, and adrenodoxin by prostaglandins E2 and F2 alpha and cholera toxin. Arch Biochem Biophys, 231(2), 271-9, 1984.

Boggaram, V, Zuber, M X, Waterman, M R. Turnover of newly synthesized cytochromes P-450scc and P-45011 beta and adrenodoxin in bovine adrenocortical cells in monolayer culture: effect of adrenocorticotropin. Arch Biochem Biophys, 231(2), 518-23, 1984.

Funkenstein, B, Waterman, M R, Simpson, E R. Induction of synthesis of cholesterol side chain cleavage cytochrome P-450 and adrenodoxin by follicle-stimulating hormone, 8-bromo-cyclic AMP, and low density lipoprotein in cultured bovine granulosa cells. J Biol Chem, 259(13), 8572-7, 1984.

John, M E, John, M C, Ashley, P, MacDonald, R J, Simpson, E R, Waterman, M R. Identification and characterization of cDNA clones specific for cholesterol side-chain cleavage cytochrome P-450. Proc Natl Acad Sci U S A, 81(18), 5628-32, 1984. PMCID:391763

John, M E, Simpson, E R, Boggaram, V, Waterman, M R. Molecular cloning of steroid hydroxylases. Endocr Res, 10(3-4), 319-33, 1984.

Kramer, R E, Rainey, W E, Funkenstein, B, Dee, A, Simpson, E R, Waterman, M R. Induction of synthesis of mitochondrial steroidogenic enzymes of bovine adrenocortical cells by analogs of cyclic AMP. J Biol Chem, 259(2), 707-13, 1984.

Matocha, M F, Waterman, M R. Discriminatory processing of the precursor forms of cytochrome P-450scc and adrenodoxin by adrenocortical and heart mitochondria. J Biol Chem, 259(13), 8672-8, 1984.

Bethlenfalvay, N C, Waterman, M R, Lima, J E, Waldrup, T. Comparative aspects of methemoglobin formation and reduction in opossum (Didelphis virginiana) and human erythrocytes. Comp Biochem Physiol A, 75(4), 635-9, 1983.

Funkenstein, B, McCarthy, J L, Dus, K M, Simpson, E R, Waterman, M R. Effect of adrenocorticotropin on steroid 21-hydroxylase synthesis and activity in cultured bovine adrenocortical cells. Increased synthesis in the absence of increased activity. J Biol Chem, 258(15), 9398-405, 1983.

Funkenstein, B, Waterman, M R, Masters, B S, Simpson, E R. Evidence for the presence of cholesterol side chain cleavage cytochrome P-450 and adrenodoxin in fresh granulosa cells. Effects of follicle-stimulating hormone and cyclic AMP on cholesterol side chain cleavage cytochrome P-450 synthesis and activity. J Biol Chem, 258(16), 10187-91, 1983.

Kramer, R E, McCarthy, J L, Simpson, E R, Waterman, M R. Effects of ACTH on steroidogenesis in bovine adrenocortical cells in primary culture--increased secretion of 17 alpha-hydroxylated steroids associated with a refractoriness in total steroid output. J Steroid Biochem, 18(6), 715-23, 1983.

Kramer, R E, Simpson, E R, Waterman, M R. Induction of 11 beta-hydroxylase by corticotropin in primary cultures of bovine adrenocortical cells. J Biol Chem, 258(5), 3000-5, 1983.

Kramer, R E, Simpson, E R, Waterman, M R. Steroidogenic refractoriness of bovine adrenocortical cells to dibutyryl cyclic AMP. Steroids, 41(2), 207-23, 1983.

McCarthy, J L, Kramer, R E, Funkenstein, B, Simpson, E R, Waterman, M R. Induction of 17 alpha-hydroxylase (cytochrome P-450(17)alpha) activity by adrenocorticotropin in bovine adrenocortical cells maintained in monolayer culture. Arch Biochem Biophys, 222(2), 590-8, 1983.

Ohashi, M, Simpson, E R, Mason, J I, Waterman, M R. Biosynthesis of cholesterol side-chain cleavage cytochrome P-450 in human fetal adrenal cells in culture. Endocrinology, 112(6), 2039-45, 1983.

Sasaki, J, Waterman, M R, Buchanan, G R, Cottam, G L. Plasma and erythrocyte lipids in sickle cell anaemia. Clin Lab Haematol, 5(1), 35-44, 1983.

Sharma, V S, Isaacson, R A, John, M E, Waterman, M R, Chevion, M. Reaction of nitric oxide with heme proteins: studies on metmyoglobin, opossum methemoglobin, and microperoxidase. Biochemistry, 22(16), 3897-902, 1983.

Simpson, E R, Waterman, M R. Regulation by ACTH of steroid hormone biosynthesis in the adrenal cortex. Can J Biochem Cell Biol, 61(7), 692-707, 1983.

Simpson, E R, Waterman, M R. Induction of synthesis of steroidogenic enzymes by corticotropin in bovine adrenocortical cells in culture. Biochem Soc Trans, 11(6), 653-5, 1983.

Waterman, M R, Estabrook, R W. The induction of microsomal electron transport enzymes. Mol Cell Biochem, 53-54(1-2), 267-78, 1983.

Bethlenfalvay, N C, Waterman, M R, Lima, J E, Waldrup, T. Cytosolic and membrane-bound methemoglobin reductases in erythrocytes of the opossum, Didelphis virginiana. Comp Biochem Physiol B, 73(3), 591-4, 1982.

John, M E, Bethlenfalvay, N C, Waterman, M R. Oxidation-reduction properties of the hemoglobin of the opossum, Didelphis virginiana. Comp Biochem Physiol B, 73(3), 585-90, 1982.

Kramer, R E, Anderson, C M, Peterson, J A, Simpson, E R, Waterman, M R. Adrenodoxin biosynthesis by bovine adrenal cells in monolayer culture. Induction by adrenocorticotropin. J Biol Chem, 257(24), 14921-5, 1982.

Kramer, R E, Du Bois, R N, Simpson, E R, Anderson, C M, Kashiwagi, K, Lambeth, J D, Jefcoate, C R, Waterman, M R. Cell-free synthesis of precursor forms of mitochondrial steroid hydroxylase enzymes of the bovine adrenal cortex. Arch Biochem Biophys, 215(2), 478-85, 1982.

Sharma, V S, John, M E, Waterman, M R. Functional studies on hemoglobin opossum. Conclusions drawn regarding the role of the distal histidine. J Biol Chem, 257(20), 11887-92, 1982.

Stone, M J, Willerson, J T, Waterman, M R. Radioimmunoassay of myoglobin. Methods Enzymol, 84, 172-7, 1982.

Waterman, M R. ACTH-mediated induction of synthesis and activity of cytochrome P-450s and related enzymes in cultured bovine adrenocortical cells. Xenobiotica, 12(11), 773-86, 1982.

DuBois, R N, Simpson, E R, Kramer, R E, Waterman, M R. Induction of synthesis of cholesterol side chain cleavage cytochrome P-450 by adrenocorticotropin in cultured bovine adrenocortical cells. J Biol Chem, 256(13), 7000-5, 1981.

DuBois, R N, Simpson, E R, Tuckey, J, Lambeth, J D, Waterman, M R. Evidence for a higher molecular weight precursor of cholesterol side-chain-cleavage cytochrome P-450 and induction of mitochondrial and cytosolic proteins by corticotropin in adult bovine adrenal cells. Proc Natl Acad Sci U S A, 78(2), 1028-32, 1981. PMCID:319939

John, M E, DuBois, R N, Waterman, M R. Altered heme environments in opossum and rabbit methemoglobins. Z Naturforsch [C], 36(11-12), 964-7, 1981.

John, M E, Waterman, M R. Spectral, conformational and chemical properties of opossum methemoglobin. Eur J Biochem, 115(1), 1-6, 1981.

John, M E, Waterman, M R. Structural basis for the conformational states of nitrosyl hemoglobins M Saskatoon and M Milwaukee. Influence of distal histidine residues on proximal histidine-iron bonds. J Biol Chem, 255(10), 4501-6, 1980.

Shibata, K, Cottam, G L, Waterman, M R. Acceleration of the rate of deoxyhemoglobin S polymerization by the erythrocyte membrane. FEBS Lett, 110(1), 107-10, 1980.

Dubois, R N, Waterman, M R. Effect of phenobarbital administration to rats on the level of the in vitro synthesis of cytochrome P-450 directed by total rat liver RNA. Biochem Biophys Res Commun, 90(1), 150-7, 1979.

Horne, M K, Waterman, M R, Simon, L M, Garriott, J C, Foerster, E H. Methemoglobinemia from sniffing butyl nitrite. Ann Intern Med, 91(3), 417-8, 1979.

John, M E, Waterman, M R. Differential effects of pH and inositol hexaphosphate on the spectroscopic properties of the alpha and beta subunits in methemoglobins M Milwaukee and A. Biochim Biophys Acta, 578(2), 269-80, 1979.

John, M E, Waterman, M R. Structural characteristics of nitrosyl hemoglobins and their relation to ESR spectra. FEBS Lett, 106(1), 219-22, 1979.

John, M E, Waterman, M R. Nitric oxide induced conformational changes in opossum hemoglobin. J Biol Chem, 254(23), 11953-7, 1979.

Russo, F M, John, M E, Waterman, M R, Simon, L M, Sheehan, R G. Hemoglobin Hofu (beta 126 Val is replaced by Glu) in a black American. Hemoglobin, 3(1), 83-6, 1979.

Waterman, M R, Cottam, G L, Shibata, K. Inhibitory effect of deoxyhemoglobin A2 on the rate of deoxyhemoglobin S polymerization. J Mol Biol, 129(2), 337-41, 1979.

Stone, M J, Waterman, M R, Poliner, L R, Templeton, G H, Buja, L M, Willerson, J T. Myoglobinemia is an early and quantitative index of acute myocardial infarction. Angiology, 29(5), 386-92, 1978.

Waterman, M R. Spectral characterization of human hemoglobin and its derivatives. Methods Enzymol, 52, 456-63, 1978.

Cottam, G L, Waterman, M R, Thompson, B C. Kinetics of polymerization of deoxyhemoglobin S and mixtures of hemoglobin A and hemoglobin S at high hemoglobin concentrations. Arch Biochem Biophys, 181(1), 61-5, 1977.

Shibata, K, Waterman, M R, Cottam, G L. Alteration of the trate of deoxyhemoglobin S polymerization. Effect of pH and percentage of oxygenation. J Biol Chem, 252(21), 7468-74, 1977.

Stone, M J, Waterman, M R, Harimoto, D, Murray, G, Willson, N, Platt, M R, Blomqvist, G, Willerson, J T. Serum myoglobin level as diagnostic test in patients with acute myocardial infarction. Br Heart J, 39(4), 375-80, 1977. PMCID:483248

Bethlenfalvay, N C, Brown, G L, Waterman, M R. Hemoglobins of the opossum (Didelphis marsupialis). II. Polymorphism; electrophoretic and chromatographic observations. Lab Anim Sci, 26(6 Pt 1), 908-12, 1976.

Cottam, G L, Waterman, M R. Effect of oxygen concentration on trasverse water proton relaxation times in erythrocytes homozygous and heterozygous for hemoglonin S. Arch Biochem Biophys, 177(1), 293-8, 1976.

Waterman, M R. Effect of carbamidomethylation of cysteine residues G11(104)alpha on the properties of hemoglobin A. Biochim Biophys Acta, 439(1), 167-74, 1976.

Waterman, M R, Cottam, G L. Kinetics of the polymerization of hemoglobin S: studies below normal erythrocyte hemoglobin concentration. Biochem Biophys Res Commun, 73(3), 639-45, 1976.

Waterman, M R, Cottam, G L. Molecular aspects of sickle cell disease. Angew Chem Int Ed Engl, 15(12), 749-57, 1976.

Chuang, A H, Waterman, M R, Yamaoka, K, Cottam, L. Effect of pH, carbamylation and other hemoglobins on deoxyhemoglobin S aggregation inside intact erythrocytes as detected by proton relaxation rate measurements. Arch Biochem Biophys, 167(1), 145-50, 1975.

Stone, M J, Willerson, J T, Gomez-Sanchez, C E, Waterman, M R. Radioimmunoassay of myoglobin in human serum. Results in patients with acute myocardial infarction. J Clin Invest, 56(5), 1334-9, 1975. PMCID:301998

Thompson, B C, Waterman, M R, Cottam, G L. Evaluation of the water environments in deoxygenated sickle cells by longitudinal and transverse water proton relaxation rates. Arch Biochem Biophys, 166(1), 193-200, 1975.

Waterman, M R, Yanaoka, K, Chuang, A H, Cottam, G L. Anti-sickling nature of dimethyl adipimidate. Biochem Biophys Res Commun, 63(3), 580-7, 1975.

Cottam, G L, Valentine, K M, Yamaoka, K, Waterman, M R. The gelation of deoxyhemoglobin S in erythrocytes as detected by transverse water proton relazation measurements. Arch Biochem Biophys, 162(2), 487-92, 1974.

Gondko, R, Obrebska, M J, Waterman, M R. Preparation and properties of a hemoglobin containing heme only in gamma subunits. Biochem Biophys Res Commun, 56(2), 444-50, 1974.

Schacter, B A, Waterman, M R. Activity of various metalloporphyrin protein complexes with microsomal heme oxygenase. Life Sci, 14(1), 47-53, 1974.

Waterman, M R. Role of cysteine residues in hemoglobin structure and function: transfer of p-mercuribenzoate from alpha subunits to beta subunits during tetramer formation. Biochim Biophys Acta, 371(1), 159-67, 1974.

Waterman, M R, Stenzel, P. Physical and chemical properties of opossum hemoglobin. A hemoglobin containing glutamine at position 58 (E7) in the alpha subunit. Biochim Biophys Acta, 359(2), 401-10, 1974.

Waterman, M R, Yamaoka, K, Dahm, L, Taylor, J, Cottam, G L. Noncovalent modification of deoxyhemoglobin S solubility and erythrocyte sickling. Proc Natl Acad Sci U S A, 71(6), 2222-5, 1974. PMCID:388423

Yamaoka, K, Cottam, G L, Waterman, M R. Spin-label studies at F9(93)beta of deoxyhemoglobin S aggregation. Biochem Biophys Res Commun, 58(4), 1058-65, 1974.

Yamaoka, K, Waterman, M R. [Letter: Are HbM Sakai and HbM Hyde Park an identical mutation form?]. Rinsho Ketsueki, 15(7), 819, 1974.

Cottam, G L, Waterman, M R. Reversible solubility of deoxyhemoglobin S. Biochem Biophys Res Commun, 54(3), 1157-63, 1973.

Painter, T D, Waterman, M R. Effect of ligands on alkaline denaturation of fetal hemoglobin and isolated gamma subunits. Biochem Biophys Res Commun, 55(3), 812-7, 1973.

Waterman, M R, Ullrich, V, Estabrook, R W. Effect of substrate on the spin state of cytochrome P-450 in hepatic microsomes. Arch Biochem Biophys, 155(2), 355-60, 1973.

Waterman, M R, Mason, H S. Redox properties of liver cytochrome P-450. Arch Biochem Biophys, 150(1), 57-63, 1972.

Waterman, M R, Gondko, R, Yonetani, T. Studies on modified hemoglobins. 1. Preparation and properties of a hemoglobin containing heme only in -chains. Arch Biochem Biophys, 145(2), 448-55, 1971.

Yonetani, T, Iizuka, T, Waterman, M R. Studies on modified hemoglobins. 3. Spin states of ferric hemoglobin, semi-hemoglobin, and isolated subunit chains. J Biol Chem, 246(24), 7683-9, 1971.

Waterman, M R, Mason, H S. The redox potential of liver cytochrome P-450. Biochem Biophys Res Commun, 39(3), 450-4, 1970.

Waterman, M R, Yonetani, T. Studies on modified hemoglobins. I. Properties of hybrid hemoglobins containing manganese protoporphyrin IX. J Biol Chem, 245(21), 5847-52, 1970.

Yonetani, T, Drott, H R, Leigh, J S, Reed, G H, Waterman, M R, Asakura, T. Electromagnetic properties of hemoproteins. 3. Electron paramagnetic resonance characteristics of iron (III) and manganese (II) protoporphyrins IX and their apohemoprotein complexes in high spin states. J Biol Chem, 245(11), 2998-3003, 1970.


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Updated Date
10/21/2008



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