Vanderbilt University School of Medicine

Hasty, Alyssa H. , Ph.D.
Professor of Molecular Physiology and Biophysics

Lab Url:

Phone Number: 322-5177 (office) / 322-5972 (lab)


Hasty, Alyssa's picture

Office Address   Mailing Address

813 LH

813 LH 0615

Research Keywords
obesity, adipose tissue macrophages, inflammation, insulin resistance, atherosclerosis,,Diabetes,Mouse,Physiology,Vascular Biology

Research Specialty
Obesity-related increases in adipose tissue macrophages leading to inflammation and insulin resistance.

Research Description
Research description:
The growing worldwide obesity epidemic is frequently linked to hyperlipidemia, inflammation, and insulin resistance leading to increased risk of diabetes and cardiovascular disease. The long-term goal of my laboratory is to determine mechanisms by which obesity increases risk for and pathophysiological consequences of these devastating diseases. Macrophages are part of the innate immune system that infiltrate white adipose tissue in obese rodents and humans, and produce most of the inflammatory cytokines and chemokines secreted from AT. In addition, their presence has been shown to be temporally associated with the development of insulin resistance. My current research focus is to determine mechanisms by which macrophages are attracted to adipose tissue and what happens to them once they arrive. Some of the current projects in my laboratory are:

Macrophage Cell Death: The macrophages in adipose tissue are exposed to high levels of fatty acids that are released from the adipocytes either due to uncontrolled lipolysis or adipocyte cell death. We have evidence in vitro, that exposure of macrophages to saturated fatty acids induced inflammation, ER stress and apoptosis. However, it has not been determined whether ATMs undergo apoptosis. In addition, apoptotic cells are normally phagocytosed by other macrophages in a process called a??efferocytosisa??. It is also now known whether efferocytosis can occur in AT in vivo. We are interested in whether efferocytosis occurs in AT and whether this can contribute to resolution of the inflammation.

Turnover of Adipose Tissue Macrophages: We have growing evidence that even in lean adipose tissue the macrophages undergo apoptotic cell death. We would like to understand how the natural turnover of macrophages contributes to the overall numbers of cells in lean and obese adipose tissue. We hypothesize that this turnover may be impaired in obesity and contribute to the accumulation of macrophages in obese adipose tissue.

Macrophage Emigration: In addition to studying mechanisms by which macrophages enter adipose tissue, we are also performing experiments to determine how their numbers decline during weight loss. This may be via increased turnover or apoptosis as described above. However, an alternative would be that they could emigrate out of adipose tissue. We have a mouse model where leukocyte emigration from tissue to nearby lymph nodes is impaired. We would like to study adipose tissue macrophage emigration in this model.

Bolus, WR, Gutierrez, DA, Kennedy, AJ, Anderson-Baucum, EK, Hasty, AH. CCR2 deficiency leads to increased eosinophils, alternative macrophage activation, and type 2 cytokine expression in adipose tissue. J Leukoc Biol, , , 2015.

Hubler, MJ, Peterson, KR, Hasty, AH. Iron homeostasis: a new job for macrophages in adipose tissue. Trends Endocrinol Metab, 26(2), 101-9, 2015.

Riley, KG, Pasek, RC, Maulis, MF, Dunn, JC, Bolus, WR, Kendall, PL, Hasty, AH, Gannon, M. Macrophages are essential for CTGF-mediated adult ??-cell proliferation after injury. Mol Metab, 4(8), 584-91, 2015.

Wainright, KS, Fleming, NJ, Rowles, JL, Welly, RJ, Zidon, TM, Park, YM, Gaines, TL, Scroggins, RJ, Anderson-Baucum, EK, Hasty, AM, Vieira-Potter, VJ, Padilla, J. Retention of sedentary obese visceral white adipose tissue phenotype with intermittent physical activity despite reduced adiposity. Am J Physiol Regul Integr Comp Physiol, , ajpregu.00042.2015, 2015.

Anderson-Baucum, EK, Major, AS, Hasty, AH. A possible secondary immune response in adipose tissue during weight cycling: The ups and downs of yo-yo dieting. Adipocyte, 3(2), 141-5, 2014.

Buckman, LB, Anderson-Baucum, EK, Hasty, AH, Ellacott, KLj. Regulation of S100B in white adipose tissue by obesity in mice. Adipocyte, 3(3), 215-20, 2014.

Hasty, AH, Gutierrez, DA. What have we really learned about macrophage recruitment to adipose tissue. Endocrinology, 155(1), 12-4, 2014.

Hill, AA, Reid Bolus, W, Hasty, AH. A decade of progress in adipose tissue macrophage biology. Immunol Rev, 262(1), 134-52, 2014.

Reddy, IA, Wasserman, DH, Ayala, JE, Hasty, AH, Abumrad, NN, Galli, A. Striatal Dopamine Homeostasis is Altered in Mice Following Roux-en-Y Gastric Bypass Surgery. ACS Chem Neurosci, , , 2014.

Anderson, EK, Gutierrez, DA, Kennedy, A, Hasty, AH. Weight cycling increases T-cell accumulation in adipose tissue and impairs systemic glucose tolerance. Diabetes, 62(9), 3180-8, 2013.

Buckman, LB, Hasty, AH, Flaherty, DK, Buckman, CT, Thompson, MM, Matlock, BK, Weller, K, Ellacott, KL. Obesity induced by a high-fat diet is associated with increased immune cell entry into the central nervous system. Brain Behav Immun, , , 2013.

Hasty, AH, Yvan-Charvet, L. Liver X Receptor ??-Dependent Iron Handling in M2 Macrophages: The Missing Link Between Cholesterol and Intraplaque Hemorrhage. Circ Res, 113(11), 1182-5, 2013.

Kang, L, Lantier, L, Kennedy, A, Bonner, JS, Mayes, WH, Bracy, DP, Bookbinder, LH, Hasty, AH, Thompson, CB, Wasserman, DH. Hyaluronan accumulates with high-fat feeding and contributes to insulin resistance. Diabetes, 62(6), 1888-96, 2013.

Kennedy, A, Webb, CD, Hill, AA, Gruen, ML, Jackson, LG, Hasty, AH. Loss of CCR5 results in glucose intolerance in diet-induced obese mice. Am J Physiol Endocrinol Metab, 305(7), E897-906, 2013.

Orr, JS, Kennedy, A, Anderson-Baucum, EK, Webb, CD, Fordahl, SC, Erikson, KM, Zhang, Y, Etzerodt, A, Moestrup, SK, Hasty, AH. Obesity Alters Adipose Tissue Macrophage Iron Content and Tissue Iron Distribution. Diabetes, , , 2013.

Orr, JS, Kennedy, AJ, Hasty, AH. Isolation of adipose tissue immune cells. J Vis Exp, (75), , 2013.

Anderson, EK, Hill, AA, Hasty, AH. Stearic Acid Accumulation in Macrophages Induces Toll-Like Receptor 4/2-Independent Inflammation Leading to Endoplasmic Reticulum Stress-Mediated Apoptosis. Arterioscler Thromb Vasc Biol, , , 2012.

Gutierrez, DA, Hasty, AH. Haematopoietic leptin receptor deficiency does not affect macrophage accumulation in adipose tissue or systemic insulin sensitivity. J Endocrinol, 212(3), 343-51, 2012. PMCID:3281060

Hasty, AH, Harrison, DG. Endoplasmic reticulum stress and hypertension - a new paradigm. J Clin Invest, 122(11), 3859-61, 2012.

Kennedy, A, Gruen, ML, Gutierrez, DA, Surmi, BK, Orr, JS, Webb, CD, Hasty, AH. Impact of macrophage inflammatory protein-1I? deficiency on atherosclerotic lesion formation, hepatic steatosis, and adipose tissue expansion. PLoS One, 7(2), e31508, 2012. PMCID:3281060

Orr, JS, Puglisi, MJ, Ellacott, KL, Lumeng, CN, Wasserman, DH, Hasty, AH. Toll-like Receptor 4 Deficiency Promotes the Alternative Activation of Adipose Tissue Macrophages. Diabetes, , , 2012.

Gutierrez, DA, Kennedy, A, Orr, JS, Anderson, EK, Webb, CD, Gerrald, WK, Hasty, AH. Aberrant accumulation of undifferentiated myeloid cells in the adipose tissue of CCR2-deficient mice delays improvements in insulin sensitivity. Diabetes, 60(11), 2820-9, 2011.

Puglisi, MJ, Hasty, AH, Saraswathi, V. The role of adipose tissue in mediating the beneficial effects of dietary fish oil. J Nutr Biochem, 22(2), 101-8, 2011.

Anderson, EK, Gutierrez, DA, Hasty, AH. Adipose tissue recruitment of leukocytes. Curr Opin Lipidol, 21(3), 172-7, 2010.

Guri, AJ, Misyak, SA, Hontecillas, R, Hasty, A, Liu, D, Si, H, Bassaganya-Riera, J. Abscisic acid ameliorates atherosclerosis by suppressing macrophage and CD4(+) T cell recruitment into the aortic wall. J Nutr Biochem, , , 2010.

Kennedy, AJ, Ellacott, KL, King, VL, Hasty, AH. Mouse models of the metabolic syndrome. Dis Model Mech, 3(3-4), 156-66, 2010.

Rudolph, TK, Rudolph, V, Edreira, MM, Cole, MP, Bonacci, G, Schopfer, FJ, Woodcock, SR, Franek, A, Pekarova, M, Khoo, NK, Hasty, AH, Baldus, S, Freeman, BA. Nitro-fatty acids reduce atherosclerosis in apolipoprotein e-deficient mice. Arterioscler Thromb Vasc Biol, 30(5), 938-45, 2010.

Surmi, BK, Webb, CD, Ristau, AC, Hasty, AH. Absence of Macrophage Inflammatory Protein-1{alpha} Does Not Impact Macrophage Accumulation in Adipose Tissue of Diet Induced Obese Mice. Am J Physiol Endocrinol Metab, , , 2010.

Batoy, SM, Borgmann, S, Flick, K, Griffith, J, Jones, JJ, Saraswathi, V, Hasty, AH, Kaiser, P, Wilkins, CL. Lipid and phospholipid profiling of biological samples using MALDI Fourier transform mass spectrometry. Lipids, 44(4), 367-71, 2009. PMCID:2615827

Coenen, KR, Gruen, ML, Lee-Young, RS, Puglisi, MJ, Wasserman, DH, Hasty, AH. Impact of macrophage toll-like receptor 4 deficiency on macrophage infiltration into adipose tissue and the artery wall in mice. Diabetologia, 52(2), 318-28, 2009. PMCID:2735041

Gutierrez, DA, Puglisi, MJ, Hasty, AH. Impact of increased adipose tissue mass on inflammation, insulin resistance, and dyslipidemia. Curr Diab Rep, 9(1), 26-32, 2009. PMCID:2735041

Li, S, Sun, Y, Liang, CP, Thorp, EB, Han, S, Jehle, AW, Saraswathi, V, Pridgen, B, Kanter, JE, Li, R, Welch, CL, Hasty, AH, Bornfeldt, KE, Breslow, JL, Tabas, I, Tall, AR. Defective phagocytosis of apoptotic cells by macrophages in atherosclerotic lesions of ob/ob mice and reversal by a fish oil diet. Circ Res, 105(11), 1072-82, 2009.

Romero-Aleshire, MJ, Diamond-Stanic, MK, Hasty, AH, Hoyer, PB, Brooks, HL. Loss of ovarian function in the VCD mouse-model of menopause leads to insulin resistance and a rapid progression into the metabolic syndrome. Am J Physiol Regul Integr Comp Physiol, 297(3), R587-92, 2009.

Saraswathi, V, Hasty, AH. Inhibition of long-chain acyl coenzyme A synthetases during fatty acid loading induces lipotoxicity in macrophages. Arterioscler Thromb Vasc Biol, 29(11), 1937-43, 2009.

Saraswathi, V, Morrow, JD, Hasty, AH. Dietary fish oil exerts hypolipidemic effects in lean and insulin sensitizing effects in obese LDLR-/- mice. J Nutr, 139(12), 2380-6, 2009.

Surmi, BK, Hasty, AH. The role of chemokines in recruitment of immune cells to the artery wall and adipose tissue. Vascul Pharmacol, 52(1-2), 27-36, 2009.

Atkinson, RD, Coenen, KR, Plummer, MR, Gruen, ML, Hasty, AH. Macrophage-derived apolipoprotein E ameliorates dyslipidemia and atherosclerosis in obese apolipoprotein E-deficient mice. Am J Physiol Endocrinol Metab, 294(2), E284-90, 2008.

Inoue, N, Yahagi, N, Yamamoto, T, Ishikawa, M, Watanabe, K, Matsuzaka, T, Nakagawa, Y, Takeuchi, Y, Kobayashi, K, Takahashi, A, Suzuki, H, Hasty, AH, Toyoshima, H, Yamada, N, Shimano, H. Cyclin-dependent Kinase Inhibitor, p21WAF1/CIP1, Is Involved in Adipocyte Differentiation and Hypertrophy, Linking to Obesity, and Insulin Resistance. J Biol Chem, 283(30), 21220-21229, 2008.

Kato, T, Shimano, H, Yamamoto, T, Ishikawa, M, Kumadaki, S, Matsuzaka, T, Nakagawa, Y, Yahagi, N, Nakakuki, M, Hasty, AH, Takeuchi, Y, Kobayashi, K, Takahashi, A, Yatoh, S, Suzuki, H, Sone, H, Yamada, N. Palmitate impairs and eicosapentaenoate restores insulin secretion through regulation of SREBP-1c in pancreatic islets. Diabetes, , , 2008. PMCID:2518489

Plummer, MR, Hasty, AH. Atherosclerotic lesion formation and triglyceride storage in obese apolipoprotein AI-deficient mice. J Nutr Biochem, , , 2008.

Stafford, JM, Yu, F, Printz, R, Hasty, AH, Swift, LL, Niswender, KD. Central nervous system neuropeptide Y signaling modulates VLDL triglyceride secretion. Diabetes, 57(6), 1482-90, 2008.

Surmi, BK, Atkinson, RD, Gruen, ML, Coenen, KR, Hasty, AH. The role of macrophage leptin receptor in aortic root lesion formation. Am J Physiol Endocrinol Metab, 294(3), E488-95, 2008.

Surmi, BK, Hasty, AH. Macrophage infiltration into adipose tissue: initiation, propagation and remodeling. Future Lipidol, 3(5), 545-556, 2008. PMCID:2575346

Coenen, KR, Gruen, ML, Chait, A, Hasty, AH. Diet-induced increases in adiposity, but not plasma lipids, promote macrophage infiltration into white adipose tissue. Diabetes, 56(3), 564-73, 2007.

Coenen, KR, Gruen, ML, Hasty, AH. Obesity causes very low density lipoprotein clearance defects in low-density lipoprotein receptor-deficient mice. J Nutr Biochem, 18(11), 727-35, 2007.

Coenen, KR, Hasty, AH. Obesity potentiates development of fatty liver and insulin resistance, but not atherosclerosis, in high-fat diet-fed agouti LDLR-deficient mice. Am J Physiol Endocrinol Metab, 293(2), E492-9, 2007.

Gruen, ML, Hao, M, Piston, DW, Hasty, AH. Leptin requires canonical migratory signaling pathways for induction of monocyte and macrophage chemotaxis. Am J Physiol Cell Physiol, 293(5), C1481-8, 2007.

Hao, M, Head, WS, Gunawardana, SC, Hasty, AH, Piston, DW. Direct effect of cholesterol on insulin secretion: a novel mechanism for pancreatic beta-cell dysfunction. Diabetes, 56(9), 2328-38, 2007.

Matsuzaka, T, Shimano, H, Yahagi, N, Kato, T, Atsumi, A, Yamamoto, T, Inoue, N, Ishikawa, M, Okada, S, Ishigaki, N, Iwasaki, H, Iwasaki, Y, Karasawa, T, Kumadaki, S, Matsui, T, Sekiya, M, Ohashi, K, Hasty, AH, Nakagawa, Y, Takahashi, A, Suzuki, H, Yatoh, S, Sone, H, Toyoshima, H, Osuga, JI, Yamada, N. Crucial role of a long-chain fatty acid elongase, Elovl6, in obesity-induced insulin resistance. Nat Med, 13(10), 1193-1202, 2007.

Nuotio-Antar, AM, Hachey, DL, Hasty, AH. Carbenoxolone treatment attenuates symptoms of metabolic syndrome and atherogenesis in obese, hyperlipidemic mice. Am J Physiol Endocrinol Metab, 293(6), E1517-28, 2007.

Saraswathi, V, Gao, L, Morrow, JD, Chait, A, Niswender, KD, Hasty, AH. Fish Oil Increases Cholesterol Storage in White Adipose Tissue with Concomitant Decreases in Inflammation, Hepatic Steatosis, and Atherosclerosis in Mice. J Nutr, 137(7), 1776-1782, 2007.

Braun, NA, Mohler, PJ, Weisgraber, KH, Hasty, AH, Linton, MF, Yancey, PG, Su, YR, Fazio, S, Swift, LL. Intracellular trafficking of recycling apolipoprotein E in Chinese hamster ovary cells. J Lipid Res, 47(6), 1176-86, 2006.

Gruen, ML, Saraswathi, V, Nuotio-Antar, AM, Plummer, MR, Coenen, KR, Hasty, AH. Plasma insulin levels predict atherosclerotic lesion burden in obese hyperlipidemic mice. Atherosclerosis, 186(1), 54-64, 2006.

Hasty, AH, Gruen, ML, Terry, ES, Surmi, BK, Atkinson, RD, Gao, L, Morrow, JD. Effects of vitamin E on oxidative stress and atherosclerosis in an obese hyperlipidemic mouse model. J Nutr Biochem, , , 2006.

Kato, T, Shimano, H, Yamamoto, T, Yokoo, T, Endo, Y, Ishikawa, M, Matsuzaka, T, Nakagawa, Y, Kumadaki, S, Yahagi, N, Takahashi, A, Sone, H, Suzuki, H, Toyoshima, H, Hasty, AH, Takahashi, S, Gomi, H, Izumi, T, Yamada, N. Granuphilin is activated by SREBP-1c and involved in impaired insulin secretion in diabetic mice. Cell Metab, 4(2), 143-54, 2006.

Liang, X, Kanjanabuch, T, Mao, SL, Hao, CM, Tang, YW, Declerck, PJ, Hasty, AH, Wasserman, DH, Fogo, AB, Ma, LJ. Plasminogen activator inhibitor-1 modulates adipocyte differentiation. Am J Physiol Endocrinol Metab, 290(1), E103-E113, 2006.

Nakagawa, Y, Shimano, H, Yoshikawa, T, Ide, T, Tamura, M, Furusawa, M, Yamamoto, T, Inoue, N, Matsuzaka, T, Takahashi, A, Hasty, AH, Suzuki, H, Sone, H, Toyoshima, H, Yahagi, N, Yamada, N. TFE3 transcriptionally activates hepatic IRS-2, participates in insulin signaling and ameliorates diabetes. Nat Med, 12(1), 107-13, 2006.

Nuotio-Antar, AM, Hasty, AH, Kovacs, WJ. Quantitation and cellular localization of 11beta-HSD1 expression in murine thymus. J Steroid Biochem Mol Biol, 99(2-3), 93-9, 2006.

Saraswathi, V, Hasty, AH. The role of lipolysis in mediating the pro-inflammatory effects of very low density lipoproteins in mouse peritoneal macrophages. J Lipid Res, , , 2006.

Wang, Y, Oeser, JK, Yang, C, Sarkar, S, Hackl, SI, Hasty, AH, McGuinness, OP, Paradee, W, Hutton, JC, Powell, DR, O''Brien, RM. Deletion of the gene encoding the ubiquitously expressed glucose-6-phosphatase catalytic subunit-related protein (UGRP)/glucose-6-phosphatase catalytic subunit-beta results in lowered plasma cholesterol and elevated glucagon. J Biol Chem, 281(52), 39982-9, 2006.

Bodary, PF, Gu, S, Shen, Y, Hasty, AH, Buckler, JM, Eitzman, DT. Recombinant leptin promotes atherosclerosis and thrombosis in apolipoprotein E-deficient mice. Arterioscler Thromb Vasc Biol, 25(8), e119-22, 2005.

Gruen, ML, Plummer, MR, Zhang, W, Posey, KA, Linton, MF, Fazio, S, Hasty, AH. Persistence of high density lipoprotein particles in obese mice lacking apolipoprotein A-I. J Lipid Res, 46(9), 2007-14, 2005.

Hasty, AH, Plummer, MR, Weisgraber, KH, Linton, MF, Fazio, S, Swift, LL. The recycling of apolipoprotein E in macrophages: influence of HDL and apolipoprotein A-I. J Lipid Res, 46(7), 1433-9, 2005.

Su, YR, Dove, DE, Major, AS, Hasty, AH, Boone, B, Linton, MF, Fazio, S. Reduced ABCA1-mediated cholesterol efflux and accelerated atherosclerosis in apolipoprotein E-deficient mice lacking macrophage-derived ACAT1. Circulation, 111(18), 2373-81, 2005.

Zhu, MY, Hasty, AH, Harris, C, Linton, MF, Fazio, S, Swift, LL. Physiological relevance of apolipoprotein E recycling: studies in primary mouse hepatocytes. Metabolism, 54(10), 1309-15, 2005.

Matsuzaka, Takashi, Shimano, Hitoshi, Yahagi, Naoya, Amemiya-Kudo, Michiyo, Okazaki, Hiroaki, Tamura, Yoshiaki, Iizuka, Yoko, Ohashi, Ken, Tomita, Sachiko, Sekiya, Motohiro, Hasty, Alyssa, Nakagawa, Yoshimi, Sone, Hirohito, Toyoshima, Hideo, Ishibashi, Shun, Osuga, Jun-Ichi, Yamada, Nobuhiro. Insulin-independent induction of sterol regulatory element-binding protein-1c expression in the livers of streptozotocin-treated mice. Diabetes, 53(3), 560-9, 2004.

Farkas, Monica H, Swift, Larry L, Hasty, Alyssa H, Linton, MacRae F, Fazio, Sergio. The recycling of apolipoprotein E in primary cultures of mouse hepatocytes. Evidence for a physiologic connection to high density lipoprotein metabolism.. J Biol Chem, 278(11), 9412-7, 2003.

Raffa??, Robert L, Hasty, Alyssa H, Wang, Yuwei, Mettler, Shelley E, Sanan, David A, Linton, MacRae F, Fazio, Sergio, Weisgraber, Karl H. Hepatocyte-derived ApoE is more effective than non-hepatocyte-derived ApoE in remnant lipoprotein clearance.. J Biol Chem, 278(13), 11670-5, 2003.

Ru Su, Yan, Ishiguro, Hiroyuki, Major, Amy S, Dove, Dwayne E, Zhang, Wenwu, Hasty, Alyssa H, Babaev, Vladimir R, Linton, MacRae F, Fazio, Sergio. Macrophage apolipoprotein A-I expression protects against atherosclerosis in ApoE-Deficient mice and up-regulates ABC transporters.. Mol Ther, 8(4), 576-83, 2003.

Yoshikawa, Tomohiro, Ide, Tomohiro, Shimano, Hitoshi, Yahagi, Naoya, Amemiya-Kudo, Michiyo, Matsuzaka, Takashi, Yatoh, Shigeru, Kitamine, Tetsuya, Okazaki, Hiroaki, Tamura, Yoshiaki, Sekiya, Motohiro, Takahashi, Akimitsu, Hasty, Alyssa H, Sato, Ryuichiro, Sone, Hirohito, Osuga, Jun-Ichi, Ishibashi, Shun, Yamada, Nobuhiro. Cross-talk between peroxisome proliferator-activated receptor (PPAR) alpha and liver X receptor (LXR) in nutritional regulation of fatty acid metabolism. I. PPARs suppress sterol regulatory element binding protein-1c promoter through inhibition of LXR signaling.. Mol Endocrinol, 17(7), 1240-54, 2003.

Amemiya-Kudo, Michiyo, Shimano, Hitoshi, Hasty, Alyssa H, Yahagi, Naoya, Yoshikawa, Tomohiro, Matsuzaka, Takashi, Okazaki, Hiroaki, Tamura, Yoshiaki, Iizuka, Yoko, Ohashi, Ken, Osuga, Jun-ichi, Harada, Kenji, Gotoda, Takanari, Sato, Ryuichiro, Kimura, Satoshi, Ishibashi, Shun, Yamada, Nobuhiro. Transcriptional activities of nuclear SREBP-1a, -1c, and -2 to different target promoters of lipogenic and cholesterogenic genes.. J Lipid Res, 43(8), 1220-35, 2002.

Fazio, Sergio, Babaev, Vladimir R, Burleigh, Michael E, Major, Amy S, Hasty, Alyssa H, Linton, MacRae F. Physiological expression of macrophage apoE in the artery wall reduces atherosclerosis in severely hyperlipidemic mice.. J Lipid Res, 43(10), 1602-9, 2002.

Matsuzaka, Takashi, Shimano, Hitoshi, Yahagi, Naoya, Amemiya-Kudo, Michiyo, Yoshikawa, Tomohiro, Hasty, Alyssa H, Tamura, Yoshiaki, Osuga, Jun-ichi, Okazaki, Hiroaki, Iizuka, Yoko, Takahashi, Akimitsu, Sone, Hirohito, Gotoda, Takanari, Ishibashi, Shun, Yamada, Nobuhiro. Dual regulation of mouse Delta(5)- and Delta(6)-desaturase gene expression by SREBP-1 and PPARalpha.. J Lipid Res, 43(1), 107-14, 2002.

Matsuzaka, Takashi, Shimano, Hitoshi, Yahagi, Naoya, Yoshikawa, Tomohiro, Amemiya-Kudo, Michiyo, Hasty, Alyssa H, Okazaki, Hiroaki, Tamura, Yoshiaki, Iizuka, Yoko, Ohashi, Ken, Osuga, Jun-Ichi, Takahashi, Akimitsu, Yato, Shigeru, Sone, Hirohito, Ishibashi, Shun, Yamada, Nobuhiro. Cloning and characterization of a mammalian fatty acyl-CoA elongase as a lipogenic enzyme regulated by SREBPs.. J Lipid Res, 43(6), 911-20, 2002.

Yahagi, Naoya, Shimano, Hitoshi, Hasty, Alyssa H, Matsuzaka, Takashi, Ide, Tomohiro, Yoshikawa, Tomohiro, Amemiya-Kudo, Michiyo, Tomita, Sachiko, Okazaki, Hiroaki, Tamura, Yoshiaki, Iizuka, Yoko, Ohashi, Ken, Osuga, Jun-Ichi, Harada, Kenji, Gotoda, Takanari, Nagai, Ryozo, Ishibashi, Shun, Yamada, Nobuhiro. Absence of sterol regulatory element-binding protein-1 (SREBP-1) ameliorates fatty livers but not obesity or insulin resistance in Lep(ob)/Lep(ob) mice.. J Biol Chem, 277(22), 19353-7, 2002.

Hasty, A H, Shimano, H, Osuga, J, Namatame, I, Takahashi, A, Yahagi, N, Perrey, S, Iizuka, Y, Tamura, Y, Amemiya-Kudo, M, Yoshikawa, T, Okazaki, H, Ohashi, K, Harada, K, Matsuzaka, T, Sone, H, Gotoda, T, Nagai, R, Ishibashi, S, Yamada, N. Severe hypercholesterolemia, hypertriglyceridemia, and atherosclerosis in mice lacking both leptin and the low density lipoprotein receptor.. J Biol Chem, 276(40), 37402-8, 2001.

Yoshida, H, Hasty, A H, Major, A S, Ishiguro, H, Su, Y R, Gleaves, L A, Babaev, V R, Linton, M F, Fazio, S. Isoform-specific effects of apolipoprotein E on atherogenesis: gene transduction studies in mice.. Circulation, 104(23), 2820-5, 2001.

Yoshikawa, T, Shimano, H, Amemiya-Kudo, M, Yahagi, N, Hasty, A H, Matsuzaka, T, Okazaki, H, Tamura, Y, Iizuka, Y, Ohashi, K, Osuga, J, Harada, K, Gotoda, T, Kimura, S, Ishibashi, S, Yamada, N. Identification of liver X receptor-retinoid X receptor as an activator of the sterol regulatory element-binding protein 1c gene promoter.. Mol Cell Biol, 21(9), 2991-3000, 2001. PMCID:86928

Amemiya-Kudo, M, Shimano, H, Yoshikawa, T, Yahagi, N, Hasty, A H, Okazaki, H, Tamura, Y, Shionoiri, F, Iizuka, Y, Ohashi, K, Osuga, J, Harada, K, Gotoda, T, Sato, R, Kimura, S, Ishibashi, S, Yamada, N. Promoter analysis of the mouse sterol regulatory element-binding protein-1c gene.. J Biol Chem, 275(40), 31078-85, 2000.

Hasty, A H, Shimano, H, Yahagi, N, Amemiya-Kudo, M, Perrey, S, Yoshikawa, T, Osuga, J, Okazaki, H, Tamura, Y, Iizuka, Y, Shionoiri, F, Ohashi, K, Harada, K, Gotoda, T, Nagai, R, Ishibashi, S, Yamada, N. Sterol regulatory element-binding protein-1 is regulated by glucose at the transcriptional level.. J Biol Chem, 275(40), 31069-77, 2000.

Fazio, S, Linton, M F, Hasty, A H, Swift, L L. Recycling of apolipoprotein E in mouse liver.. J Biol Chem, 274(12), 8247-53, 1999.

Hasty, A H, Linton, M F, Brandt, S J, Babaev, V R, Gleaves, L A, Fazio, S. Retroviral gene therapy in ApoE-deficient mice: ApoE expression in the artery wall reduces early foam cell lesion formation.. Circulation, 99(19), 2571-6, 1999.

Hasty, A H, Linton, M F, Swift, L L, Fazio, S. Determination of the lower threshold of apolipoprotein E resulting in remnant lipoprotein clearance.. J Lipid Res, 40(8), 1529-38, 1999.

Shimano, H, Yahagi, N, Amemiya-Kudo, M, Hasty, A H, Osuga, J, Tamura, Y, Shionoiri, F, Iizuka, Y, Ohashi, K, Harada, K, Gotoda, T, Ishibashi, S, Yamada, N. Sterol regulatory element-binding protein-1 as a key transcription factor for nutritional induction of lipogenic enzyme genes.. J Biol Chem, 274(50), 35832-9, 1999.

Yahagi, N, Shimano, H, Hasty, A H, Amemiya-Kudo, M, Okazaki, H, Tamura, Y, Iizuka, Y, Shionoiri, F, Ohashi, K, Osuga, J, Harada, K, Gotoda, T, Nagai, R, Ishibashi, S, Yamada, N. A crucial role of sterol regulatory element-binding protein-1 in the regulation of lipogenic gene expression by polyunsaturated fatty acids.. J Biol Chem, 274(50), 35840-4, 1999.

Linton, M F, Hasty, A H, Babaev, V R, Fazio, S. Hepatic apo E expression is required for remnant lipoprotein clearance in the absence of the low density lipoprotein receptor.. J Clin Invest, 101(8), 1726-36, 1998. PMCID:508755

Fazio, S, Babaev, V R, Murray, A B, Hasty, A H, Carter, K J, Gleaves, L A, Atkinson, J B, Linton, M F. Increased atherosclerosis in mice reconstituted with apolipoprotein E null macrophages.. Proc Natl Acad Sci U S A, 94(9), 4647-52, 1997. PMCID:20778

Fazio, S, Hasty, A H, Carter, K J, Murray, A B, Price, J O, Linton, M F. Leukocyte low density lipoprotein receptor (LDL-R) does not contribute to LDL clearance in vivo: bone marrow transplantation studies in the mouse.. J Lipid Res, 38(2), 391-400, 1997.

Postdoctoral Position Available

Postdoctoral Position Details
The overarching goal of the Hasty Laboratory is to determine mechanisms by which obesity increases risk of metabolic disease. We are focused primarily on the role that resident and recruited immune cells play in adipose tissue function in the lean and obese states. We are seeking a highly motivated, energetic, creative thinking person to join our team as a postdoctoral fellow. Candidates with experience in general immunological techniques such as flow cytometry and/or familiarity with cellular iron handling will be given special consideration. Please contact Alyssa Hasty (, providing your CV and contact information for individuals who will provide reference letters.

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