Vanderbilt University School of Medicine

Chiang, Chin , Ph.D.
Professor of Cell and Developmental Biology

Lab Url: http://www.mc.vanderbilt.edu/vumcdept/cellbio/chiang

Phone Number: (615) 343-4922

Email Address:chin.chiang@vanderbilt.edu

Chiang, Chin's picture

Office Address   Mailing Address

4110 Medical Research Building 3

4114, MRB3 37232-8240


Research Specialty
Hedgehog signaling in development and cancer

Research Description
Our research focuses on the role Sonic hedgehog signaling plays in the pathogenesis of the two most common types of pediatric cancers, soft tissue sarcomas and brain tumors. We study the tumor cells-of-origin, initiation and propagation. In addition, we are also interested in identifying tumor biomarkers and compounds that inhibit their growth. We use state of the art approaches that include stem cell manipulation, molecular genetics, orthotopic grafting, proteomics, genome-wide RNA sequencing and CHIP.

Another area of interest is to understand how the complex brain circuitry is established from a rather small number of neural stem cells. We study signaling pathways that regulate brain stem cell proliferation and differentiation.

Publications
Bebesh, A.E., Fleming, J.T., Chiang, C., Carter, B.D. and Tyska, M.J.. Expression and localization of myosin-1d in the developing nervous system. Brain Res., 1440, 9, 2012.

Cheng, F.Y., Huang, X., Sarangi, A., Ketova, T., Cooper, M.K., Litingtung, Y. and Chiang, C.. Widespread contribution of Gdf7 lineage to cerebellar cell types and implications for Hedgehog-driven medulloblastoma formation.. Plos One, 7, 35541, 2012.

Huang, X., Dubuc, A.M., Hashizume, R., Berg, J., He, Y., Chiang, C. Cooper, M.K., Northcott, P.A., Yatlor, M.D., Barnes, M.J., Tijan, T., Chen, J., Hackett, C.S., Weiss, W.A., David James, C., Rowitch, D.H., Shuman, M.A., Jan, Y.H. and Jan L.Y. . Voltage-gated potassium channel EAG2 controls mitotic entry and tumor growth in medulloblastoma via regulating cell volume dynamics.. Genes Dev., 26, 1780, 2012.

Ryan K.E. and Chiang, C.. Hedgehog Secretion and Signal Transduction in Vertebrates. J. Biol. Chem., 287, 179015, 2012.

Mason, T.A., Kolobova, E., Liu, J., Roland, J.T., Chiang, C.. Darinaparsin is a multivalent chemotherapeutic which induces incomplete stress response with disruption of microtubules and Shh signaling. Plos One, 6(11), 27699, 2011.

Huang, X, Liu, J, Ketova, T, Fleming, JT, Grover, VK, Cooper, MK, Litingtung, Y, Chiang, C. Transventricular delivery of Sonic hedgehog is essential to cerebellar ventricular zone development. Proc Natl Acad Sci U S A, 107(18), 8422-7, 2010.

Huang, X., Ketova, T., Litingtung, Y. and Chiang, C. Isolation, Enrichment and Maintenance of Medulloblastoma Stem Cells. . J. Vis. Exp., 43, 2086, 2010.

Huang, X., Sarangi, A., Ketova, T., Cooper, M. K., Litingtung, Y. and Chiang, C. . Intracranial Orthotopic Allografting of Medulloblastoma. . J. Vis. Exp., 44, 2153, 2010.

Huang, X, Ketova, T, Fleming, JT, Wang, H, Dey, SK, Litingtung, Y, Chiang, C. Sonic hedgehog signaling regulates a novel epithelial progenitor domain of the hindbrain choroid plexus. Development, , , 2009.

Oh, S, Huang, X, Liu, J, Litingtung, Y, Chiang, C. Shh and Gli3 activities are required for timely generation of motor neuron progenitors. Dev Biol, 331(2), 261-9, 2009. PMCID:2703695

Fogel, JL, Chiang, C, Huang, X, Agarwala, S. Ventral specification and perturbed boundary formation in the mouse midbrain in the absence of Hedgehog signaling. Dev Dyn, 237(5), 1359-72, 2008. PMCID:2586936

Huang, X, Goudy, SL, Ketova, T, Litingtung, Y, Chiang, C. Gli3-deficient mice exhibit cleft palate associated with abnormal tongue development. Dev Dyn, 237(10), 3079-87, 2008.

Li, Y, Gordon, J, Manley, NR, Litingtung, Y, Chiang, C. Bmp4 is required for tracheal formation: A novel mouse model for tracheal agenesis. Dev Biol, , , 2008.

Guerrero, I and Chiang, C.. A conserved mechanism of Hedgehog gradient formation by lipid modifications. Trends in Cell Biology, 17, 1-5, 2007.

Huang, X, Litingtung, Y, Chiang, C. Region-specific requirement for cholesterol modification of sonic hedgehog in patterning the telencephalon and spinal cord. Development, 134(11), 2095-105, 2007.

Huang, X, Litingtung, Y, Chiang, C. Ectopic sonic hedgehog signaling impairs telencephalic dorsal midline development: implication for human holoprosencephaly. Hum Mol Genet, 16(12), 1454-68, 2007. PMCID:1988863

Li, Y, Litingtung, Y, Ten Dijke, P, Chiang, C. Aberrant Bmp signaling and notochord delamination in the pathogenesis of esophageal atresia. Dev Dyn, 236(3), 746-54, 2007.

Zhang, H, Lawson, WE, Polosukhin, VV, Pozzi, A, Blackwell, TS, Litingtung, Y, Chiang, C. Inhibitor of differentiation 1 promotes endothelial survival in a bleomycin model of lung injury in mice. Am J Pathol, 171(4), 1113-26, 2007. PMCID:1988863

Chiang, C. From Oligodactyly to Polydactyly:role of Shh and Gli3 in limb morphogenesis. In Hedgehog-Gli Signaling and Human Disease, Edited by A. Ruiz I Altaba . , , 137-145, 2006.

Li, Y, Zhang, H, Litingtung, Y, Chiang, C. Cholesterol modification restricts the spread of Shh gradient in the limb bud. Proc Natl Acad Sci U S A, 103(17), 6548-53, 2006. PMCID:1458921

Zhang, H., Litingtung, Y. and Chiang, C.. Sonic Hedgehog signaling in viceral organ development. In Shh and Gli signaling in Development. Edited by S. Howie and C. Fisher. , , , 2006.

Oh, Saeock, Huang, Xi, Chiang, Chin. Specific requirements of sonic hedgehog signaling during oligodendrocyte development. Dev Dyn, 234(3), 489-96, 2005.

van Bokhoven, H, Celli, J, van Reeuwijk, J, Rinne, T, Glaudemans, B, van Beusekom, E, Rieu, P, Newbury-Ecob, RA, Chiang, C, Brunner, HG. MYCN haploinsufficiency is associated with reduced brain size and intestinal atresias in Feingold syndrome. Nat Genet, 37(5), 465-7, 2005.

Li, Yina, Zhang, Huimin, Choi, Seung Cheol, Litingtung, Ying, Chiang, Chin. Sonic hedgehog signaling regulates Gli3 processing, mesenchymal proliferation, and differentiation during mouse lung organogenesis. Dev Biol, 270(1), 214-31, 2004.

Powles, N, Marshall, H, Economou, A, Chiang, C, Murakami, A, Dickson, C, Krumlauf, R, Maconochie, M. Regulatory analysis of the mouse Fgf3 gene: control of embryonic expression patterns and dependence upon sonic hedgehog (Shh) signalling. Dev Dyn, 230(1), 44-56, 2004.

Chiang, CW, Kanies, C, Kim, KW, Fang, WB, Parkhurst, C, Xie, M, Henry, T, Yang, E. Protein phosphatase 2A dephosphorylation of phosphoserine 112 plays the gatekeeper role for BAD-mediated apoptosis. Mol Cell Biol, 23(18), 6350-62, 2003. PMCID:193703

Litingtung, Ying, Dahn, Randall D, Li, Yina, Fallon, John F, Chiang, Chin. Shh and Gli3 are dispensable for limb skeleton formation but regulate digit number and identity. Nature, 418(6901), 979-83, 2002.

Liu, W, Li, G, Chien, JS, Raft, S, Zhang, H, Chiang, C, Frenz, DA. Sonic hedgehog regulates otic capsule chondrogenesis and inner ear development in the mouse embryo. Dev Biol, 248(2), 240-50, 2002.

Ohkubo, Y, Chiang, C, Rubenstein, JL. Coordinate regulation and synergistic actions of BMP4, SHH and FGF8 in the rostral prosencephalon regulate morphogenesis of the telencephalic and optic vesicles. Neuroscience, 111(1), 1-17, 2002.

Perriton, CL, Powles, N, Chiang, C, Maconochie, MK, Cohn, MJ. Sonic hedgehog signaling from the urethral epithelium controls external genital development. Dev Biol, 247(1), 26-46, 2002.

Chattopadhyay, A, Chiang, CW, Yang, E. BAD/BCL-[X(L)] heterodimerization leads to bypass of G0/G1 arrest. Oncogene, 20(33), 4507-18, 2001.

Chiang, C, Litingtung, Y, Harris, M P, Simandl, B K, Li, Y, Beachy, P A, Fallon, J F. Manifestation of the limb prepattern: limb development in the absence of sonic hedgehog function. Dev Biol, 236(2), 421-35, 2001.

Chiang, CW, Harris, G, Ellig, C, Masters, SC, Subramanian, R, Shenolikar, S, Wadzinski, BE, Yang, E. Protein phosphatase 2A activates the proapoptotic function of BAD in interleukin- 3-dependent lymphoid cells by a mechanism requiring 14-3-3 dissociation. Blood, 97(5), 1289-97, 2001.

Mo, R, Kim, JH, Zhang, J, Chiang, C, Hui, CC, Kim, PC. Anorectal malformations caused by defects in sonic hedgehog signaling. Am J Pathol, 159(2), 765-74, 2001. PMCID:1850556

Litingtung, Y, Chiang, C. Control of Shh activity and signaling in the neural tube. Dev Dyn, 219(2), 143-54, 2000.

Litingtung, Y, Chiang, C. Specification of ventral neuron types is mediated by an antagonistic interaction between Shh and Gli3. Nat Neurosci, 3(10), 979-85, 2000.

Pak, JH, Huang, FL, Li, J, Balschun, D, Reymann, KG, Chiang, C, Westphal, H, Huang, KP. Involvement of neurogranin in the modulation of calcium/calmodulin-dependent protein kinase II, synaptic plasticity, and spatial learning: a study with knockout mice. Proc Natl Acad Sci U S A, 97(21), 11232-7, 2000. PMCID:17183

Borycki, AG, Brunk, B, Tajbakhsh, S, Buckingham, M, Chiang, C, Emerson, CP. Sonic hedgehog controls epaxial muscle determination through Myf5 activation. Development, 126(18), 4053-63, 1999.

Chiang, C, Swan, RZ, Grachtchouk, M, Bolinger, M, Litingtung, Y, Robertson, EK, Cooper, MK, Gaffield, W, Westphal, H, Beachy, PA, Dlugosz, AA. Essential role for Sonic hedgehog during hair follicle morphogenesis. Dev Biol, 205(1), 1-9, 1999.

Pierani, A, Brenner-Morton, S, Chiang, C, Jessell, TM. A sonic hedgehog-independent, retinoid-activated pathway of neurogenesis in the ventral spinal cord. Cell, 97(7), 903-15, 1999.

Kos, L, Chiang, C, Mahon, KA. Mediolateral patterning of somites: multiple axial signals, including Sonic hedgehog, regulate Nkx-3.1 expression. Mech Dev, 70(1-2), 25-34, 1998.

Litingtung, Y, Lei, L, Westphal, H, Chiang, C. Sonic hedgehog is essential to foregut development. Nat Genet, 20(1), 58-61, 1998.

Knezevic, V., De Santo, R., Schughart, K., Huffstadt, U., Chiang, C., Mahon, K.A. and Mackem, S. Hoxd-12 differentially affects preaxial and postaxial chondrogenic branches in the limb and regulates sonic hedgehog in a positive feedback loop. Development, 124, 4523-4536, 1997.

Chiang C, Litingtung Y, Lee E, Young K, Corden JL, Westphal H and Beachy PA. Cyclopia and defective axial patterning in mice lacking Sonic Hedgehog gene function. Nature, 383, 407-413, 1996.

Yuryev, A, Patturajan, M, Litingtung, Y, Joshi, RV, Gentile, C, Gebara, M, Corden, JL. The C-terminal domain of the largest subunit of RNA polymerase II interacts with a novel set of serine/arginine-rich proteins. Proc Natl Acad Sci U S A, 93(14), 6975-80, 1996. PMCID:38919

Chiang, C., Yong, K.E., and Beachy, P.A.. Controlof drosphila trachael branching by the novel homeodomain gene unplugges, a regulatory target for genes of the bithorax complex.. Development, 121, 3901-3912, 1995.

Fan, C.-M., Porter, J.A., Chiang, C., Chang, D.T., Beachy, P.A., Tessier,-Lavigne, M.. Long range sclerotome induction by Sonic Hedgehog: direct role of the amino-terminal clleavage product and modulation by the cyclic AMP signalling pathway. Cell, 81, 457-465, 1995.

Hynes, M., Porter, J.A., Chiang, C, Chang, D., Tessier-Lavigne, M., Beachy P.A., and Rosenthal, A.. Induction of midbrain dopaminergic neurons product of hedgehog autoproteolysis. Neuron, 15, 35-44, 1995.

Lopez, A., Chang, D.T., Chiang, C., Porter, J., maria, A.R., Simandl, K., Beachy, P.A., and Fallon, J.F.. Limb patterning activity and restricted posterior localization of the amino terminal product of hedgehog autoproteolysis. Current Biology, 5, 791-796, 1995.

Roelink, H., Porter, J., Chiang, C., Tanabe, Y., Chang, D.T., Beachy, P.A., and Jessell, T.M.. Concentration-dependent induction of floor plate and motor neuron differentiation by the amino terminal autoproteolytic fragment of Sonic Hedgehog.. Cell, 81, 445-455, 1995.

Chang, D.T., Lopez, A., von Kessler, D.P., Chiang, C., Simandl, K., Renbin, Z., Seldin, M.F., Fallon, J.F., and Beachy, P.A. Products, genetic linkage, and limb patterning activity of a murine hedgehog gene.. Development, 120, 3339-3353, 1994.

Chiang, C., andBeachy, P.A. Expression of a novel Toll-like gene spans the parasegment boundary and contributes to hedgehog function in the adult eye of Drosophila. Mech. Dev., 47, 225-239, 1994.

Chiang, C.,, Patel, N., Young, K., and Beachy, P.A.. The novel homeodomain gene buttonless specifies differentiation and axonal guidance functions of Drosophila dorsal median cells. Development, 120, 3581-3593, 1994.


Postdoctoral Position Available
Yes

Postdoctoral Position Details
Contact Chin Chiang at chin.chiang@vanderbilt.edu for more details.

Updated Date
08/23/2013



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